Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0012222

Physical Location & Seq

Genomic Coordinates Reverse (-)

1975816 .. 1979866

Gene Structure 4051 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1027600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGGTCAAGCACCCTGCGGCCCCGGTGAAGCATAAGCATTACAGGCCTGCTGGAAAGAAAAAGGAAGGAAATGCCGCGAGGTACGTCACCAGGTCACAAGCTGTCAAGCAGCTTCAAGTCAGCCTTCCCGTCTTCAGGAAATTGTGTATTCTAAAGGGTGTTTTCCCTCGGGAGCCGAAGAAAAAGGTGAAAGGAAATCACCATACTTATTATCACTTGAAGGATGTTTCTTTCATTCAGCATGAACCGCTACTCGAGCGGTTGAGAGAGATCAGGGCATACGAAAGGAAAGTAAAGAAAGCCGATGCGAAGAAGAACAAGGAGCGCGCTGCTCTTCTGAAAGAGAGAAGACCCACTTACAAATTAGATAGAATTATTTTGCAGAGGTATCCGAAATTTGTTGACGCACTTAGAGATATAGACGACTGCCTCACAATGGTTCACCTCTTTGCAGCCTTACCTGCTATTGAGGATAGAATTGATGTGAAACGCATTCATAACTGTCGGAGGTTGAGTCATGAATGGCAAGCTTATATATCTCGGTCTCATAAATTGCGTAAAGTCTTTGTATCTGTTAAAGGCATATATTATCAGGCCGAAGTTAAGGGGCAAAAAGTCACATGGTTGACCCCTCACCCTTTGCAGCAAGTTTTGACTGATGATATCGACTTCAATATCATGCTTAACTTTTTGGAATTCTATGAGGCTCTTCTTGCCTTTGTGAACTGTCATTTATATCGTTCCATAGATGTGAAGTACCCACCCATTCTTGATTCTCGATTGGAAGCTTTAGCAGCAGAACTTTATGCACTTGCAAGATACCTCGATGCAAATTCTCGGTCCGCTGTATTGGATCCTCAAGCTTCTAGTTTGTCTGAATCTGGGAGTGGTGAGAACAAGAAGATTGAGACTTCGGTTGAAGAGTCTGAACTAAGACTTGCCCAACTTCAACATCAACTGCCTTCGAATGAACCAGGTGCATTAATGCACCTTGTTAAAGATGTTTCTAGCGAGGATGAAGAGGATAATGATACAAAGGAGTGTAAGCAACTCTTCAAGGATATGAAATTTTTCTTGAACCGTGAGGTTTACAGAGAGTCATTGCTTTTCGTCATTCCTGCTTTTGGTGGCACTGTTTCTTGGGAAGGTGATGGGGCTCCATTTGAGGAAGCTGATGACAGTATTACCCATCAGATTGTTGACAGGCCAATGCATGGTCGTCAGACCCTTGCCAGGGAATACGTTCAACCACAATGGGTTTATGACTGTATAAATGCTCGGATCATTTTGCCTACTGGGGGTTATTTGGTGGGAAGGGATCCTCCCCCACACTTGTCACCATTTGTTGACAATGAGGCAGAAGGTTATGTTCCGGATTATGCTGAGACCATAAAAAAATGGAAGTCTGCTGGTGAAGTCCTCCCCCTGCCAGGAGTGGGACAAGAAGATTTGGAAGATCCTCAGAATTTACTGGCTGAAGGTGTCATTGACCGAGCAAAAGCAAAAGAAGCTGTGGAGAGAAAGAAGAAGATGATGGCTCTTGAGAAGCAGTATCATGACGAATTGAATATGGAGCTTCAGGGCATCCAACATCCATCATCTACCTCGAATGTAGATAATCAGAACTCTGCCAAGGACACCACCGAGGAGGTGGAAGAGTTTGATCCTGATACTGATCGTGACAATATGTCTAAAATGTTAATGTCCCGTAAGAAGAGGGGCGTGCTTGAAGCTATGGAGAAAGGCAAAAAACGAAAGAAGGACCACGTCGATGTCATCAGGGAACGGAAGAGAAAACTTAAAGAATCCCAAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000463 GO:0000470 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0010468 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0022613 GO:0030684 GO:0030686 GO:0031323 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0070545 GO:0071704 GO:0071840 GO:0080090 GO:0090069 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904 GO:2000232

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRCT_2 Pescadillo_N

Protein Analysis

606

Amino Acids

69.8

Weight (kDa)

8.79

Isoelectric Point (pI)

47.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pescadillo_N	PF06732	19 - 280	1.3e-110	Pescadillo N-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14520
fragaria_vesca	FvH4_3g26080 FvH4_3g26080 FvH4_3g26080
malus_domestica	MD09G1027600.v1.1 MD17G1027600.v1.1
prunus_persica	Prupe.3G291100_v2.0.a1
pyrus_communis	pycom111g02170 pycom17g02260
rosa_chinensis	RchiOBHm_Chr5g0047391
rosa_laevigata	RLG00000034492
rosa_multiflora	Rmu_sc0000580.1_g000062 Rmu_sc0003441.1_g000056
rosa_roxburghii	Rroxscaffold_1G00034010
rosa_rugosa	Rorug05G0236100
rosa_samantha	Rh5AG314900 Rh5CG350800 Rh5DG334700
rosa_wichuraiana	Rw5G029430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	469
AccB7I	CCANNNNNTGG	1 cut(s)	1214
AccBSI	CCGCTC	1 cut(s)	259
AccII	CGCG	2 cut(s)	77, 327
AccIII	TCCGGA	1 cut(s)	1372
AciI	CCGC	5 cut(s)	17, 75, 248, 259, 843
AclWI	GGATC	7 cut(s)	848, 861, 1289, 1313, 1326, 1451, 1658
AcsI	RAATTY	5 cut(s)	395, 695, 832, 1067, 1465
AcuI	CTGAAG	3 cut(s)	118, 1497, 1562
AfaI	GTAC	2 cut(s)	83, 758
AfiI	CCNNNNNNNGG	9 cut(s)	135, 436, 1124, 1214, 1233, 1234, 1298, 1430, 1436
AhdI	GACNNNNNGTC	1 cut(s)	1687
AjiI	CACGTC	1 cut(s)	1768
AjnI	CCWGG	4 cut(s)	89, 973, 1232, 1429
AluBI	AGCT	9 cut(s)	101, 112, 530, 788, 863, 1172, 1511, 1576, 1733
AluI	AGCT	9 cut(s)	101, 112, 530, 788, 863, 1172, 1511, 1576, 1733
Alw26I	GTCTC	3 cut(s)	549, 902, 1379
AlwI	GGATC	7 cut(s)	848, 861, 1289, 1313, 1326, 1451, 1658
Ama87I	CYCGRG	2 cut(s)	168, 254
Aor13HI	TCCGGA	1 cut(s)	1372
AoxI	GGCC	4 cut(s)	18, 44, 594, 1205
ApeKI	GCWGC	5 cut(s)	109, 329, 452, 643, 794
ApoI	RAATTY	5 cut(s)	395, 695, 832, 1067, 1465
ArsI	GACNNNNNNTTYG	2 cut(s)	824, 856
AseI	ATTAAT	1 cut(s)	983
Asp700I	GAANNNNTTC	2 cut(s)	1070, 1242
AspLEI	GCGC	2 cut(s)	327, 329
AspS9I	GGNCC	3 cut(s)	19, 840, 1762
AsuC2I	CCSGG	1 cut(s)	23
AsuII	TTCGAA	1 cut(s)	965
AvaI	CYCGRG	2 cut(s)	168, 254
AvaII	GGWCC	2 cut(s)	840, 1762
BamHI	GGATCC	2 cut(s)	853, 1318
BanII	GRGCYC	1 cut(s)	1159
BbsI	GAAGAC	2 cut(s)	124, 355
BbvI	GCAGC	5 cut(s)	121, 316, 464, 655, 806
BccI	CCATC	4 cut(s)	1145, 1197, 1528, 1603
BciT130I	CCWGG	4 cut(s)	91, 975, 1234, 1431
BciVI	GTATCC	1 cut(s)	399
BcnI	CCSGG	1 cut(s)	23
BcoDI	GTCTC	3 cut(s)	549, 902, 1379
BfaI	CTAG	2 cut(s)	867, 1008
BfuAI	ACCTGC	1 cut(s)	469
BfuI	GTATCC	1 cut(s)	399
BisI	GCNGC	7 cut(s)	18, 75, 110, 330, 453, 644, 795
BlsI	GCNGC	7 cut(s)	19, 76, 111, 331, 454, 645, 796
Bme1390I	CCNGG	5 cut(s)	23, 91, 975, 1234, 1431
Bme18I	GGWCC	2 cut(s)	840, 1762
BmeRI	GACNNNNNGTC	1 cut(s)	1687
BmeT110I	CYCGRG	2 cut(s)	168, 254
BmgBI	CACGTC	1 cut(s)	1768
BmgT120I	GGNCC	3 cut(s)	19, 840, 1762
BmiI	GGNNCC	5 cut(s)	21, 174, 855, 1158, 1320
BmrFI	CCNGG	5 cut(s)	23, 91, 975, 1234, 1431
BmrI	ACTGGG	1 cut(s)	1305
BmsI	GCATC	3 cut(s)	295, 817, 1593
BmuI	ACTGGG	1 cut(s)	1305
BpiI	GAAGAC	2 cut(s)	124, 355
Bpu14I	TTCGAA	1 cut(s)	965
BpuEI	CTTGAG	2 cut(s)	843, 1562
BpuMI	CCSGG	1 cut(s)	23
BsaBI	GATNNNNATC	1 cut(s)	1674
BsaI	GGTCTC	2 cut(s)	549, 1379
BsaJI	CCNNGG	5 cut(s)	21, 167, 1233, 1632, 1644
BsaWI	WCCGGW	1 cut(s)	1372
BsaXI	ACNNNNNCTCC	2 cut(s)	499, 529
Bsc4I	CCNNNNNNNGG	9 cut(s)	135, 436, 1124, 1214, 1233, 1234, 1298, 1430, 1436
Bse1I	ACTGG	2 cut(s)	1300, 1476
Bse3DI	GCAATG	1 cut(s)	1100
Bse8I	GATNNNNATC	1 cut(s)	1674
BseAI	TCCGGA	1 cut(s)	1372
BseBI	CCWGG	4 cut(s)	91, 975, 1234, 1431
BseDI	CCNNGG	5 cut(s)	21, 167, 1233, 1632, 1644
BseGI	GGATG	4 cut(s)	229, 1021, 1584, 1591
BseJI	GATNNNNATC	1 cut(s)	1674
BseLI	CCNNNNNNNGG	9 cut(s)	135, 436, 1124, 1214, 1233, 1234, 1298, 1430, 1436
BseMI	GCAATG	1 cut(s)	1100
BseMII	CTCAG	2 cut(s)	1374, 1475
BseNI	ACTGG	2 cut(s)	1300, 1476
BsePI	GCGCGC	1 cut(s)	325
BseRI	GAGGAG	1 cut(s)	1661
BseXI	GCAGC	5 cut(s)	121, 316, 464, 655, 806
Bsh1236I	CGCG	2 cut(s)	77, 327
BshFI	GGCC	4 cut(s)	20, 46, 596, 1207
BsiHKCI	CYCGRG	2 cut(s)	168, 254
BsiSI	CCGG	2 cut(s)	23, 1373
BslFI	GGGAC	2 cut(s)	1452, 1690
BslI	CCNNNNNNNGG	9 cut(s)	135, 436, 1124, 1214, 1233, 1234, 1298, 1430, 1436
BsmAI	GTCTC	3 cut(s)	549, 902, 1379
BsmFI	GGGAC	2 cut(s)	1452, 1690
BsmI	GAATGC	1 cut(s)	492
BsnI	GGCC	4 cut(s)	20, 46, 596, 1207
Bso31I	GGTCTC	2 cut(s)	549, 1379
BsoBI	CYCGRG	2 cut(s)	168, 254
Bsp119I	TTCGAA	1 cut(s)	965
Bsp1286I	GDGCHC	1 cut(s)	1159
Bsp13I	TCCGGA	1 cut(s)	1372
Bsp143I	GATC	7 cut(s)	270, 853, 1281, 1318, 1456, 1663, 1675
BspACI	CCGC	5 cut(s)	17, 75, 248, 259, 843
BspANI	GGCC	4 cut(s)	20, 46, 596, 1207
BspCNI	CTCAG	2 cut(s)	1375, 1474
BspEI	TCCGGA	1 cut(s)	1372
BspFNI	CGCG	2 cut(s)	77, 327
BspHI	TCATGA	2 cut(s)	517, 1555
BspLI	GGNNCC	5 cut(s)	21, 174, 855, 1158, 1320
BspMI	ACCTGC	1 cut(s)	469
BspPI	GGATC	7 cut(s)	848, 861, 1289, 1313, 1326, 1451, 1658
BspQI	GCTCTTC	2 cut(s)	339, 714
BspT104I	TTCGAA	1 cut(s)	965
BspTNI	GGTCTC	2 cut(s)	549, 1379
BsrBI	CCGCTC	1 cut(s)	259
BsrDI	GCAATG	1 cut(s)	1100
BsrI	ACTGG	2 cut(s)	1300, 1476
BssECI	CCNNGG	5 cut(s)	21, 167, 1233, 1632, 1644
BssHII	GCGCGC	1 cut(s)	325
BssMI	GATC	7 cut(s)	270, 853, 1281, 1318, 1456, 1663, 1675
BssT1I	CCWWGG	1 cut(s)	1632
Bst2UI	CCWGG	4 cut(s)	91, 975, 1234, 1431
Bst4CI	ACNGT	6 cut(s)	503, 728, 1082, 1135, 1181, 1268
Bst6I	CTCTTC	8 cut(s)	339, 714, 915, 1014, 1058, 1650, 1709, 1784
BstBI	TTCGAA	1 cut(s)	965
BstC8I	GCNNGC	4 cut(s)	48, 327, 528, 1724
BstDEI	CTNAG	4 cut(s)	410, 932, 1383, 1461
BstF5I	GGATG	4 cut(s)	229, 1021, 1584, 1591
BstFNI	CGCG	2 cut(s)	77, 327
BstHHI	GCGC	2 cut(s)	327, 329
BstKTI	GATC	7 cut(s)	273, 856, 1284, 1321, 1459, 1666, 1678
BstMAI	GTCTC	3 cut(s)	549, 902, 1379
BstMBI	GATC	7 cut(s)	270, 853, 1281, 1318, 1456, 1663, 1675
BstMWI	GCNNNNNNNGC	7 cut(s)	43, 247, 256, 461, 713, 794, 1508
BstNI	CCWGG	4 cut(s)	91, 975, 1234, 1431
BstSCI	CCNGG	5 cut(s)	21, 89, 973, 1232, 1429
BstUI	CGCG	2 cut(s)	77, 327
BstV1I	GCAGC	5 cut(s)	121, 316, 464, 655, 806
BstV2I	GAAGAC	2 cut(s)	124, 355
BstX2I	RGATCY	3 cut(s)	853, 1318, 1456
BstYI	RGATCY	3 cut(s)	853, 1318, 1456
BsuI	GTATCC	1 cut(s)	399
BsuRI	GGCC	4 cut(s)	20, 46, 596, 1207
BtrI	CACGTC	1 cut(s)	1768
BtsCI	GGATG	4 cut(s)	229, 1021, 1584, 1591
BtsIMutI	CAGTG	1 cut(s)	1131
BveI	ACCTGC	1 cut(s)	469
Cac8I	GCNNGC	4 cut(s)	48, 327, 528, 1724
CciI	TCATGA	2 cut(s)	517, 1555
CfoI	GCGC	2 cut(s)	327, 329
Cfr13I	GGNCC	3 cut(s)	19, 840, 1762
CpoI	CGGWCCG	1 cut(s)	840
CseI	GACGC	1 cut(s)	413
CsiI	ACCWGGT	2 cut(s)	89, 973
Csp6I	GTAC	2 cut(s)	82, 757
CspI	CGGWCCG	1 cut(s)	840
CviAII	CATG	6 cut(s)	242, 518, 621, 679, 1214, 1556
CviQI	GTAC	2 cut(s)	82, 757
DdeI	CTNAG	4 cut(s)	410, 932, 1383, 1461
DpnI	GATC	7 cut(s)	272, 855, 1283, 1320, 1458, 1665, 1677
DpnII	GATC	7 cut(s)	270, 853, 1281, 1318, 1456, 1663, 1675
DriI	GACNNNNNGTC	1 cut(s)	1687
Eam1104I	CTCTTC	8 cut(s)	339, 714, 915, 1014, 1058, 1650, 1709, 1784
Eam1105I	GACNNNNNGTC	1 cut(s)	1687
EarI	CTCTTC	8 cut(s)	339, 714, 915, 1014, 1058, 1650, 1709, 1784
Eco130I	CCWWGG	1 cut(s)	1632
Eco147I	AGGCCT	1 cut(s)	46
Eco24I	GRGCYC	1 cut(s)	1159
Eco31I	GGTCTC	2 cut(s)	549, 1379
Eco32I	GATATC	1 cut(s)	664
Eco47I	GGWCC	2 cut(s)	840, 1762
Eco57I	CTGAAG	3 cut(s)	118, 1497, 1562
Eco88I	CYCGRG	2 cut(s)	168, 254
EcoRI	GAATTC	1 cut(s)	695
EcoRII	CCWGG	4 cut(s)	89, 973, 1232, 1429
EcoRV	GATATC	1 cut(s)	664
EcoT14I	CCWWGG	1 cut(s)	1632
EcoT22I	ATGCAT	1 cut(s)	1215
EcoT38I	GRGCYC	1 cut(s)	1159
ErhI	CCWWGG	1 cut(s)	1632
FaeI	CATG	6 cut(s)	245, 521, 624, 682, 1217, 1559
FalI	AAGNNNNNCTT	6 cut(s)	108, 140, 340, 372, 1037, 1069
FaqI	GGGAC	2 cut(s)	1452, 1690
FatI	CATG	6 cut(s)	241, 517, 620, 678, 1213, 1555
Fnu4HI	GCNGC	7 cut(s)	18, 75, 110, 330, 453, 644, 795
FokI	GGATG	4 cut(s)	236, 1028, 1571, 1578
FriOI	GRGCYC	1 cut(s)	1159
Fsp4HI	GCNGC	7 cut(s)	18, 75, 110, 330, 453, 644, 795
FspBI	CTAG	2 cut(s)	867, 1008
GlaI	GCGC	2 cut(s)	326, 328
GluI	GCNGC	7 cut(s)	18, 75, 110, 330, 453, 644, 795
HaeIII	GGCC	4 cut(s)	20, 46, 596, 1207
HapII	CCGG	2 cut(s)	23, 1373
HgaI	GACGC	1 cut(s)	413
HhaI	GCGC	2 cut(s)	327, 329
Hin1II	CATG	6 cut(s)	245, 521, 624, 682, 1217, 1559
Hin6I	GCGC	2 cut(s)	325, 327
HinP1I	GCGC	2 cut(s)	325, 327
HincII	GTYRAC	4 cut(s)	403, 627, 1201, 1348
HindII	GTYRAC	4 cut(s)	403, 627, 1201, 1348
HindIII	AAGCTT	3 cut(s)	528, 786, 861
HinfI	GANTC	6 cut(s)	514, 773, 878, 923, 1097, 1805
HpaII	CCGG	2 cut(s)	23, 1373
Hpy166II	GTNNAC	7 cut(s)	403, 442, 627, 724, 1090, 1201, 1348
Hpy8I	GTNNAC	7 cut(s)	403, 442, 627, 724, 1090, 1201, 1348
Hpy99I	CGWCG	1 cut(s)	1772
HpyAV	CCTTC	9 cut(s)	59, 134, 214, 972, 1139, 1308, 1355, 1472, 1753
HpyCH4III	ACNGT	6 cut(s)	503, 728, 1082, 1135, 1181, 1268
HpyCH4IV	ACGT	3 cut(s)	84, 1242, 1767
HpyCH4V	TGCA	9 cut(s)	382, 452, 643, 809, 815, 830, 980, 988, 1213
HpyF10VI	GCNNNNNNNGC	7 cut(s)	43, 247, 256, 461, 713, 794, 1508
HpyF3I	CTNAG	4 cut(s)	410, 932, 1383, 1461
HpySE526I	ACGT	3 cut(s)	84, 1242, 1767
Hsp92II	CATG	6 cut(s)	245, 521, 624, 682, 1217, 1559
HspAI	GCGC	2 cut(s)	325, 327
Kpn2I	TCCGGA	1 cut(s)	1372
Kzo9I	GATC	7 cut(s)	270, 853, 1281, 1318, 1456, 1663, 1675
LguI	GCTCTTC	2 cut(s)	339, 714
LmnI	GCTCC	4 cut(s)	172, 322, 1162, 1573
Lsp1109I	GCAGC	5 cut(s)	121, 316, 464, 655, 806
LweI	GCATC	3 cut(s)	295, 817, 1593
MabI	ACCWGGT	2 cut(s)	89, 973
MaeI	CTAG	2 cut(s)	867, 1008
MaeII	ACGT	3 cut(s)	84, 1242, 1767
MaeIII	GTNAC	5 cut(s)	85, 93, 616, 1335, 1679
MalI	GATC	7 cut(s)	272, 855, 1283, 1320, 1458, 1665, 1677
MbiI	CCGCTC	1 cut(s)	259
MboI	GATC	7 cut(s)	270, 853, 1281, 1318, 1456, 1663, 1675
MflI	RGATCY	3 cut(s)	853, 1318, 1456
MhlI	GDGCHC	1 cut(s)	1159
MlyI	GAGTC	3 cut(s)	523, 932, 1106
MmeI	TCCRAC	2 cut(s)	485, 1612
Mph1103I	ATGCAT	1 cut(s)	1215
MroI	TCCGGA	1 cut(s)	1372
MroXI	GAANNNNTTC	2 cut(s)	1070, 1242
MseI	TTAA	7 cut(s)	576, 603, 684, 983, 996, 1700, 1800
MslI	CAYNNNNRTG	2 cut(s)	749, 1770
MspA1I	CMGCKG	1 cut(s)	845
MspI	CCGG	2 cut(s)	23, 1373
MspR9I	CCNGG	5 cut(s)	23, 91, 975, 1234, 1431
Mva1269I	GAATGC	1 cut(s)	492
MvaI	CCWGG	4 cut(s)	91, 975, 1234, 1431
MvnI	CGCG	2 cut(s)	77, 327
MwoI	GCNNNNNNNGC	7 cut(s)	43, 247, 256, 461, 713, 794, 1508
NciI	CCSGG	1 cut(s)	23
NdeII	GATC	7 cut(s)	270, 853, 1281, 1318, 1456, 1663, 1675
NlaIII	CATG	6 cut(s)	245, 521, 624, 682, 1217, 1559
NlaIV	GGNNCC	5 cut(s)	21, 174, 855, 1158, 1320
NmuCI	GTSAC	5 cut(s)	85, 93, 616, 1335, 1679
NsiI	ATGCAT	1 cut(s)	1215
NspV	TTCGAA	1 cut(s)	965
PaeR7I	CTCGAG	1 cut(s)	254
PagI	TCATGA	2 cut(s)	517, 1555
PauI	GCGCGC	1 cut(s)	325
PceI	AGGCCT	1 cut(s)	46
PciSI	GCTCTTC	2 cut(s)	339, 714
PctI	GAATGC	1 cut(s)	492
PdmI	GAANNNNTTC	2 cut(s)	1070, 1242
PfeI	GAWTC	3 cut(s)	773, 878, 1805
PflFI	GACNNNGTC	1 cut(s)	1766
PflMI	CCANNNNNTGG	1 cut(s)	1214
PkrI	GCNGC	7 cut(s)	19, 76, 111, 331, 454, 645, 796
PleI	GAGTC	3 cut(s)	522, 931, 1105
PpsI	GAGTC	3 cut(s)	522, 931, 1105
PshBI	ATTAAT	1 cut(s)	983
Psp6I	CCWGG	4 cut(s)	89, 973, 1232, 1429
PspGI	CCWGG	4 cut(s)	89, 973, 1232, 1429
PspN4I	GGNNCC	5 cut(s)	21, 174, 855, 1158, 1320
PspPI	GGNCC	3 cut(s)	19, 840, 1762
PspXI	VCTCGAGB	1 cut(s)	254
PsuI	RGATCY	3 cut(s)	853, 1318, 1456
PsyI	GACNNNGTC	1 cut(s)	1766
PteI	GCGCGC	1 cut(s)	325
RsaI	GTAC	2 cut(s)	83, 758
RsaNI	GTAC	2 cut(s)	82, 757
RseI	CAYNNNNRTG	2 cut(s)	749, 1770
Rsr2I	CGGWCCG	1 cut(s)	840
RsrII	CGGWCCG	1 cut(s)	840
SapI	GCTCTTC	2 cut(s)	339, 714
SaqAI	TTAA	7 cut(s)	576, 603, 684, 983, 996, 1700, 1800
SatI	GCNGC	7 cut(s)	18, 75, 110, 330, 453, 644, 795
Sau3AI	GATC	7 cut(s)	270, 853, 1281, 1318, 1456, 1663, 1675
Sau96I	GGNCC	3 cut(s)	19, 840, 1762
SchI	GAGTC	3 cut(s)	523, 932, 1106
ScrFI	CCNGG	5 cut(s)	23, 91, 975, 1234, 1431
SduI	GDGCHC	1 cut(s)	1159
SexAI	ACCWGGT	2 cut(s)	89, 973
SfaNI	GCATC	3 cut(s)	295, 817, 1593
Sfr274I	CTCGAG	1 cut(s)	254
SfuI	TTCGAA	1 cut(s)	965
SinI	GGWCC	2 cut(s)	840, 1762
SlaI	CTCGAG	1 cut(s)	254
SmiMI	CAYNNNNRTG	2 cut(s)	749, 1770
SmlI	CTYRAG	3 cut(s)	254, 858, 1541
SmoI	CTYRAG	3 cut(s)	254, 858, 1541
SseBI	AGGCCT	1 cut(s)	46
SsiI	CCGC	5 cut(s)	17, 75, 248, 259, 843
SspMI	CTAG	2 cut(s)	867, 1008
StuI	AGGCCT	1 cut(s)	46
StyD4I	CCNGG	5 cut(s)	21, 89, 973, 1232, 1429
StyI	CCWWGG	1 cut(s)	1632
TaaI	ACNGT	6 cut(s)	503, 728, 1082, 1135, 1181, 1268
TaiI	ACGT	3 cut(s)	87, 1245, 1770
TaqI	TCGA	7 cut(s)	255, 666, 778, 825, 965, 1607, 1770
TaqII	GACCGA	3 cut(s)	531, 828, 1506
TauI	GCSGC	2 cut(s)	20, 77
TfiI	GAWTC	3 cut(s)	773, 878, 1805
Tru1I	TTAA	7 cut(s)	576, 603, 684, 983, 996, 1700, 1800
Tru9I	TTAA	7 cut(s)	576, 603, 684, 983, 996, 1700, 1800
TscAI	CASTG	1 cut(s)	1138
TseFI	GTSAC	5 cut(s)	85, 93, 616, 1335, 1679
TseI	GCWGC	5 cut(s)	109, 329, 452, 643, 794
Tsp45I	GTSAC	5 cut(s)	85, 93, 616, 1335, 1679
TspDTI	ATGAA	7 cut(s)	223, 258, 485, 534, 984, 1032, 1079
TspGWI	ACGGA	1 cut(s)	1801
TspRI	CASTG	1 cut(s)	1138
Tth111I	GACNNNGTC	1 cut(s)	1766
Van91I	CCANNNNNTGG	1 cut(s)	1214
VpaK11BI	GGWCC	2 cut(s)	840, 1762
VspI	ATTAAT	1 cut(s)	983
XapI	RAATTY	5 cut(s)	395, 695, 832, 1067, 1465
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1648
XhoI	CTCGAG	1 cut(s)	254
XmnI	GAANNNNTTC	2 cut(s)	1070, 1242
XspI	CTAG	2 cut(s)	867, 1008
Zsp2I	ATGCAT	1 cut(s)	1215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.