MD09G1030400.v1.1
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0020136
Physical Location & Seq
Forward (+)
1859410 .. 1862067
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1030400.v1.1.491

Sequence Viewer

Length: 498 bp
ATGCGAATTCTGATGGTGGGTCTCGATGCAGCCGGTAAGACCACCATCTTGTACAAACTCAAGCTTGGAGAAATCGTCACCACCATCCCCACCATTGGATTTAATGTGGAGACAGTGGAATACAAGAACATCAGCTTCACCGTCTGGGATGTCGGGGGTCAAGACAAGATCCGTCCATTGTGGAGGCACTACTTCCAGAACACACAAGGTCTTATCTTTGTGGTTGATAGCAATGATAGGGATCGAGTTGTTGAGGCAAGGGATGAATTGCACAGGATGTTGAATGAGGAGGATGAACTAAGGGATGCTGTTCTGCTTGTATTTGCCAACAAACAAGATCTTCCTAATGCAATGAATGCCGCCGAGATAACTGATAAGCTTGGTCTTCATTCTCTCCGTCAACGTCACTGGTACATCCAGAGCACTTGTGCAACCTCTGGAGAGGGTCTGTACGAGGGGTTGGAATGGCTTTCCAACAACATTGCTAGCAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko05110 ko05134 map04072 map04144 map05110 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

166

Amino Acids

18.81

Weight (kDa)

5.37

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 1 - 161 6.8e-77 ADP-ribosylation factor family
Roc PF08477 2 - 113 9.1e-14 Ras of Complex, Roc, domain of DAPkinase
SRPRB PF09439 2 - 127 2.9e-13 Signal recognition particle receptor beta subunit
Ras PF00071 2 - 160 2.6e-12 Ras family
Gtr1_RagA PF04670 2 - 125 2.2e-10 Gtr1/RagA G protein conserved region
G-alpha PF00503 28 - 94 8.6e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020136)

Species Orthologous Gene IDs
malus_domestica MD09G1030400.v1.1
prunus_persica Prupe.3G287000_v2.0.a1
pyrus_communis pycom17g02980
rosa_multiflora Rmu_sc0036527.1_g000002
rosa_samantha Rh4AG406000 Rh4BG417000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 360
AclWI GGATC 2 cut(s) 163, 249
AcsI RAATTY 1 cut(s) 6
AfaI GTAC 3 cut(s) 53, 413, 452
AfiI CCNNNNNNNGG 1 cut(s) 95
AgsI TTSAA 1 cut(s) 283
AluBI AGCT 3 cut(s) 64, 135, 379
AluI AGCT 3 cut(s) 64, 135, 379
Alw21I GWGCWC 1 cut(s) 425
Alw26I GTCTC 2 cut(s) 26, 104
AlwI GGATC 2 cut(s) 163, 249
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 6
AsuHPI GGTGA 2 cut(s) 70, 130
AsuNHI GCTAGC 1 cut(s) 485
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 425
BbvI GCAGC 1 cut(s) 41
BccI CCATC 3 cut(s) 7, 53, 92
BcoDI GTCTC 2 cut(s) 26, 104
BfaI CTAG 1 cut(s) 486
BglII AGATCT 1 cut(s) 337
BisI GCNGC 2 cut(s) 30, 360
BlsI GCNGC 2 cut(s) 31, 361
BmsI GCATC 2 cut(s) 16, 295
BmtI GCTAGC 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 44
BsaBI GATNNNNATC 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 26
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 3 cut(s) 238, 357, 480
Bse8I GATNNNNATC 1 cut(s) 240
BseGI GGATG 7 cut(s) 84, 154, 268, 282, 298, 310, 414
BseJI GATNNNNATC 1 cut(s) 240
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMI GCAATG 3 cut(s) 238, 357, 480
BseNI ACTGG 1 cut(s) 413
BseRI GAGGAG 1 cut(s) 302
BseXI GCAGC 1 cut(s) 41
BsiHKAI GWGCWC 1 cut(s) 425
BsiSI CCGG 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 2 cut(s) 26, 104
BsmI GAATGC 1 cut(s) 361
Bso31I GGTCTC 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 51
Bsp143I GATC 3 cut(s) 168, 241, 337
BspACI CCGC 1 cut(s) 360
BspOI GCTAGC 1 cut(s) 489
BspPI GGATC 2 cut(s) 163, 249
BspTNI GGTCTC 1 cut(s) 26
BsrDI GCAATG 3 cut(s) 238, 357, 480
BsrFI RCCGGY 1 cut(s) 32
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 32
BssMI GATC 3 cut(s) 168, 241, 337
Bst4CI ACNGT 2 cut(s) 115, 142
BstAPI GCANNNNNTGC 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 487
BstDEI CTNAG 1 cut(s) 299
BstF5I GGATG 7 cut(s) 84, 154, 268, 282, 298, 310, 414
BstKTI GATC 3 cut(s) 171, 244, 340
BstMAI GTCTC 2 cut(s) 26, 104
BstMBI GATC 3 cut(s) 168, 241, 337
BstMWI GCNNNNNNNGC 2 cut(s) 356, 491
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 2 cut(s) 168, 337
BstYI RGATCY 2 cut(s) 168, 337
BtsCI GGATG 7 cut(s) 84, 154, 268, 282, 298, 310, 414
BtsIMutI CAGTG 2 cut(s) 120, 406
Cac8I GCNNGC 1 cut(s) 487
Cfr10I RCCGGY 1 cut(s) 32
Csp6I GTAC 3 cut(s) 52, 412, 451
CviAII CATG 1 cut(s) 495
CviJI RGCY 5 cut(s) 32, 64, 135, 379, 469
CviKI_1 RGCY 5 cut(s) 32, 64, 135, 379, 469
CviQI GTAC 3 cut(s) 52, 412, 451
DdeI CTNAG 1 cut(s) 299
DpnI GATC 3 cut(s) 170, 243, 339
DpnII GATC 3 cut(s) 168, 241, 337
Eco31I GGTCTC 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 6
FaeI CATG 1 cut(s) 498
FaiI YATR 1 cut(s) 496
FatI CATG 1 cut(s) 494
Fnu4HI GCNGC 2 cut(s) 30, 360
FokI GGATG 7 cut(s) 71, 161, 275, 289, 305, 317, 401
Fsp4HI GCNGC 2 cut(s) 30, 360
FspBI CTAG 1 cut(s) 486
GluI GCNGC 2 cut(s) 30, 360
GsuI CTGGAG 1 cut(s) 459
HapII CCGG 1 cut(s) 33
Hin1II CATG 1 cut(s) 498
HincII GTYRAC 1 cut(s) 401
HindII GTYRAC 1 cut(s) 401
HindIII AAGCTT 2 cut(s) 62, 377
HpaII CCGG 1 cut(s) 33
HphI GGTGA 2 cut(s) 70, 130
Hpy166II GTNNAC 1 cut(s) 401
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 5 cut(s) 23, 161, 196, 418, 438
Hpy8I GTNNAC 1 cut(s) 401
HpyCH4III ACNGT 2 cut(s) 115, 142
HpyCH4IV ACGT 1 cut(s) 403
HpyCH4V TGCA 4 cut(s) 29, 271, 350, 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 356, 491
HpyF3I CTNAG 1 cut(s) 299
HpySE526I ACGT 1 cut(s) 403
Hsp92II CATG 1 cut(s) 498
Kzo9I GATC 3 cut(s) 168, 241, 337
LpnPI CCDG 7 cut(s) 46, 130, 209, 259, 394, 423, 431
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 2 cut(s) 16, 295
MaeI CTAG 1 cut(s) 486
MaeII ACGT 1 cut(s) 403
MaeIII GTNAC 2 cut(s) 76, 404
MalI GATC 3 cut(s) 170, 243, 339
MboI GATC 3 cut(s) 168, 241, 337
MboII GAAGA 2 cut(s) 332, 377
MflI RGATCY 2 cut(s) 168, 337
MhlI GDGCHC 1 cut(s) 425
MluCI AATT 2 cut(s) 6, 266
MmeI TCCRAC 2 cut(s) 441, 498
MnlI CCTC 7 cut(s) 177, 247, 280, 283, 436, 445, 448
MseI TTAA 1 cut(s) 102
MspI CCGG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 361
MwoI GCNNNNNNNGC 2 cut(s) 356, 491
NdeII GATC 3 cut(s) 168, 241, 337
NheI GCTAGC 1 cut(s) 485
NlaIII CATG 1 cut(s) 498
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 2 cut(s) 76, 404
PctI GAATGC 1 cut(s) 361
PkrI GCNGC 2 cut(s) 31, 361
PsuI RGATCY 2 cut(s) 168, 337
RsaI GTAC 3 cut(s) 53, 413, 452
RsaNI GTAC 3 cut(s) 52, 412, 451
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 2 cut(s) 30, 360
Sau3AI GATC 3 cut(s) 168, 241, 337
SduI GDGCHC 1 cut(s) 425
SetI ASST 6 cut(s) 66, 137, 211, 381, 406, 437
SfaNI GCATC 2 cut(s) 16, 295
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 2 cut(s) 6, 266
SsiI CCGC 1 cut(s) 360
SspMI CTAG 1 cut(s) 486
TaaI ACNGT 2 cut(s) 115, 142
TaiI ACGT 1 cut(s) 406
TaqI TCGA 2 cut(s) 24, 244
TasI AATT 2 cut(s) 6, 266
TatI WGTACW 1 cut(s) 51
TauI GCSGC 1 cut(s) 362
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 2 cut(s) 120, 413
TseFI GTSAC 2 cut(s) 76, 404
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 2 cut(s) 76, 404
TspDTI ATGAA 4 cut(s) 279, 309, 368, 377
TspGWI ACGGA 2 cut(s) 161, 386
TspRI CASTG 2 cut(s) 120, 413
XapI RAATTY 1 cut(s) 6
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.