pycom17g02980
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0020136
Physical Location & Seq
Forward (+)
1965487 .. 1967389
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g02980.1

Sequence Viewer

Length: 495 bp
ATGCGAATTCTGATGGTGGGTCTCGATGCAGCCGGTAAGACCACCATCTTGTACAAGCTCAAGCTCGGAGAAATCGTCACCACCATCCCCACCATCGGATTTAATGTGGAGACAGTTGAATACAAGAACATCAGCTTCACCGTTTGGGATGTCGGGGGTCAAGACAAAATCCGACCATTGTGGAGGCACTACTTCCAGAACACACAAGGTCTTATCTTTGTGGTTGATAGCAATGACAGGGATCGAGTTGTTGAGGCAAGGGATGAATTACACAGGATGTTGAATGAGGATGAACTAAGGGATGCTGTTCTGCTTGTATTTGCCAACAAACAAGATCGATCTAATGCAATGAATGCCGCCGAGATAACTGATAAGCTTGGTCTTCATTCTCTCCGTCAACGTCACTGGTACATCCAGAGTACTTGTGCAACCTCTGGAGAGGGTTTGTACGAGGGGTTGGAGTGGCTTTCCAACAACATTGCTAGCAAGGCATGA

Protein Analysis

165

Amino Acids

18.71

Weight (kDa)

5.8

Isoelectric Point (pI)

33.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 1 - 160 1.7e-77 ADP-ribosylation factor family
Roc PF08477 2 - 112 6.2e-14 Ras of Complex, Roc, domain of DAPkinase
SRPRB PF09439 2 - 126 8.5e-13 Signal recognition particle receptor beta subunit
Ras PF00071 2 - 159 9.5e-12 Ras family
Gtr1_RagA PF04670 2 - 124 1.1e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 28 - 94 7.4e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020136)

Species Orthologous Gene IDs
malus_domestica MD09G1030400.v1.1
prunus_persica Prupe.3G287000_v2.0.a1
pyrus_communis pycom17g02980
rosa_multiflora Rmu_sc0036527.1_g000002
rosa_samantha Rh4AG406000 Rh4BG417000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 357
AclWI GGATC 1 cut(s) 249
AcsI RAATTY 1 cut(s) 6
AfaI GTAC 4 cut(s) 53, 410, 421, 449
AfiI CCNNNNNNNGG 1 cut(s) 95
AgsI TTSAA 2 cut(s) 119, 283
AluBI AGCT 4 cut(s) 58, 64, 135, 376
AluI AGCT 4 cut(s) 58, 64, 135, 376
Alw26I GTCTC 2 cut(s) 26, 104
AlwI GGATC 1 cut(s) 249
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 6
AsuHPI GGTGA 2 cut(s) 70, 130
AsuNHI GCTAGC 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 374
BbvI GCAGC 1 cut(s) 41
BccI CCATC 4 cut(s) 7, 53, 92, 101
BcoDI GTCTC 2 cut(s) 26, 104
BfaI CTAG 1 cut(s) 483
BisI GCNGC 2 cut(s) 30, 357
BlsI GCNGC 2 cut(s) 31, 358
BmcAI AGTACT 1 cut(s) 421
BmsI GCATC 2 cut(s) 16, 292
BmtI GCTAGC 1 cut(s) 486
BpiI GAAGAC 1 cut(s) 374
BpmI CTGGAG 1 cut(s) 456
BpuEI CTTGAG 1 cut(s) 44
Bsa29I ATCGAT 1 cut(s) 337
BsaI GGTCTC 1 cut(s) 26
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 410
Bse3DI GCAATG 3 cut(s) 238, 354, 477
BseCI ATCGAT 1 cut(s) 337
BseGI GGATG 7 cut(s) 84, 154, 268, 282, 295, 307, 411
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMI GCAATG 3 cut(s) 238, 354, 477
BseNI ACTGG 1 cut(s) 410
BseXI GCAGC 1 cut(s) 41
BshVI ATCGAT 1 cut(s) 337
BsiSI CCGG 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 2 cut(s) 26, 104
BsmI GAATGC 1 cut(s) 358
Bso31I GGTCTC 1 cut(s) 26
Bsp1407I TGTACA 1 cut(s) 51
Bsp143I GATC 3 cut(s) 241, 334, 338
BspACI CCGC 1 cut(s) 357
BspDI ATCGAT 1 cut(s) 337
BspOI GCTAGC 1 cut(s) 486
BspPI GGATC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 26
BsrDI GCAATG 3 cut(s) 238, 354, 477
BsrFI RCCGGY 1 cut(s) 32
BsrGI TGTACA 1 cut(s) 51
BsrI ACTGG 1 cut(s) 410
BssAI RCCGGY 1 cut(s) 32
BssMI GATC 3 cut(s) 241, 334, 338
Bst4CI ACNGT 2 cut(s) 115, 142
BstAPI GCANNNNNTGC 1 cut(s) 353
BstAUI TGTACA 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 484
BstDEI CTNAG 1 cut(s) 296
BstF5I GGATG 7 cut(s) 84, 154, 268, 282, 295, 307, 411
BstKTI GATC 3 cut(s) 244, 337, 341
BstMAI GTCTC 2 cut(s) 26, 104
BstMBI GATC 3 cut(s) 241, 334, 338
BstMWI GCNNNNNNNGC 2 cut(s) 353, 488
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 1 cut(s) 374
Bsu15I ATCGAT 1 cut(s) 337
BsuTUI ATCGAT 1 cut(s) 337
BtsCI GGATG 7 cut(s) 84, 154, 268, 282, 295, 307, 411
BtsIMutI CAGTG 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 32
ClaI ATCGAT 1 cut(s) 337
Csp6I GTAC 4 cut(s) 52, 409, 420, 448
CviAII CATG 1 cut(s) 492
CviJI RGCY 6 cut(s) 32, 58, 64, 135, 376, 466
CviKI_1 RGCY 6 cut(s) 32, 58, 64, 135, 376, 466
CviQI GTAC 4 cut(s) 52, 409, 420, 448
DdeI CTNAG 1 cut(s) 296
DpnI GATC 3 cut(s) 243, 336, 340
DpnII GATC 3 cut(s) 241, 334, 338
Eco31I GGTCTC 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 6
FaeI CATG 1 cut(s) 495
FaiI YATR 1 cut(s) 493
FatI CATG 1 cut(s) 491
Fnu4HI GCNGC 2 cut(s) 30, 357
FokI GGATG 7 cut(s) 71, 161, 275, 289, 302, 314, 398
Fsp4HI GCNGC 2 cut(s) 30, 357
FspBI CTAG 1 cut(s) 483
GluI GCNGC 2 cut(s) 30, 357
GsuI CTGGAG 1 cut(s) 456
HapII CCGG 1 cut(s) 33
Hin1II CATG 1 cut(s) 495
HincII GTYRAC 1 cut(s) 398
HindII GTYRAC 1 cut(s) 398
HindIII AAGCTT 1 cut(s) 374
HpaII CCGG 1 cut(s) 33
HphI GGTGA 2 cut(s) 70, 130
Hpy166II GTNNAC 1 cut(s) 398
Hpy188I TCNGA 4 cut(s) 12, 68, 98, 173
Hpy188III TCNNGA 5 cut(s) 23, 161, 196, 415, 435
Hpy8I GTNNAC 1 cut(s) 398
HpyCH4III ACNGT 2 cut(s) 115, 142
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 3 cut(s) 29, 347, 428
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 488
HpyF3I CTNAG 1 cut(s) 296
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 1 cut(s) 495
Kzo9I GATC 3 cut(s) 241, 334, 338
LpnPI CCDG 7 cut(s) 46, 209, 223, 259, 391, 420, 428
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 2 cut(s) 16, 292
MaeI CTAG 1 cut(s) 483
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 2 cut(s) 76, 401
MalI GATC 3 cut(s) 243, 336, 340
MboI GATC 3 cut(s) 241, 334, 338
MboII GAAGA 1 cut(s) 374
MluCI AATT 2 cut(s) 6, 266
MmeI TCCRAC 3 cut(s) 196, 438, 495
MnlI CCTC 6 cut(s) 177, 247, 280, 433, 442, 445
MseI TTAA 1 cut(s) 102
MspI CCGG 1 cut(s) 33
Mva1269I GAATGC 1 cut(s) 358
MwoI GCNNNNNNNGC 2 cut(s) 353, 488
NdeII GATC 3 cut(s) 241, 334, 338
NheI GCTAGC 1 cut(s) 482
NlaIII CATG 1 cut(s) 495
NmeAIII GCCGAG 1 cut(s) 385
NmuCI GTSAC 2 cut(s) 76, 401
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 1 cut(s) 358
PkrI GCNGC 2 cut(s) 31, 358
RsaI GTAC 4 cut(s) 53, 410, 421, 449
RsaNI GTAC 4 cut(s) 52, 409, 420, 448
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 2 cut(s) 30, 357
Sau3AI GATC 3 cut(s) 241, 334, 338
ScaI AGTACT 1 cut(s) 421
SetI ASST 7 cut(s) 60, 66, 137, 211, 378, 403, 434
SfaNI GCATC 2 cut(s) 16, 292
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 2 cut(s) 6, 266
SsiI CCGC 1 cut(s) 357
SspMI CTAG 1 cut(s) 483
TaaI ACNGT 2 cut(s) 115, 142
TaiI ACGT 1 cut(s) 403
TaqI TCGA 3 cut(s) 24, 244, 337
TasI AATT 2 cut(s) 6, 266
TatI WGTACW 2 cut(s) 51, 419
TauI GCSGC 1 cut(s) 359
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 410
TseFI GTSAC 2 cut(s) 76, 401
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 2 cut(s) 76, 401
TspDTI ATGAA 4 cut(s) 279, 306, 365, 374
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 1 cut(s) 410
XapI RAATTY 1 cut(s) 6
XspI CTAG 1 cut(s) 483
ZrmI AGTACT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.