Rh4AG406000
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0020136
Physical Location & Seq
Forward (+)
70754960 .. 70758542
3583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG406000.1

Sequence Viewer

Length: 402 bp
ATGGGGCTCACGTTCACGAAGCTTTTCAGCCGGCTTTTTGCCAAGAAAGAGATGCGAATTCTGATGGTCGGTCTCGATGCCGCTGGTAAGACCACCATTCTCTACAAGCTCAAGCTCGGTGAGATCGTCACAACAATTCCTACTATTGGATTTAATGTTGAGACCGTGGAATACAAGAACATCAGCTTCACAGTCTGGGATGTCGGGGGTCAGGACAAGATCCGACCTTTGTGGAGGCACTACTTCCAGAACACACAGGGTCTTATCTTTGTTGTGGACAGCAATGACAGAGACCGTGTTGTTGAGGCAAGGGATGAATTGCACAGGATGTTGAATGAGGTTTGTTCTTATTTTGCAAGAGCACTTTTACAGACATGCTTTAACTTTTTATTCTTGCAGTAA

Protein Analysis

133

Amino Acids

15.48

Weight (kDa)

8.97

Isoelectric Point (pI)

23.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 5 - 113 1.3e-52 ADP-ribosylation factor family
SRPRB PF09439 16 - 105 2.6e-10 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 109 6.4e-13 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 116 2.5e-09 Ras family
Gtr1_RagA PF04670 19 - 118 2.7e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 44 - 111 3.2e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020136)

Species Orthologous Gene IDs
malus_domestica MD09G1030400.v1.1
prunus_persica Prupe.3G287000_v2.0.a1
pyrus_communis pycom17g02980
rosa_multiflora Rmu_sc0036527.1_g000002
rosa_samantha Rh4AG406000 Rh4BG417000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 81
AclWI GGATC 1 cut(s) 214
AcsI RAATTY 1 cut(s) 57
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 1 cut(s) 334
AluBI AGCT 4 cut(s) 22, 109, 115, 186
AluI AGCT 4 cut(s) 22, 109, 115, 186
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 3 cut(s) 77, 155, 285
AlwI GGATC 1 cut(s) 214
ApoI RAATTY 1 cut(s) 57
Asp700I GAANNNNTTC 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 131
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 364
BccI CCATC 1 cut(s) 58
BcoDI GTCTC 3 cut(s) 77, 155, 285
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
BmsI GCATC 2 cut(s) 42, 67
BpuEI CTTGAG 1 cut(s) 95
BsaI GGTCTC 3 cut(s) 77, 155, 285
BsaJI CCNNGG 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 289
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 3 cut(s) 205, 319, 333
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMI GCAATG 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 3 cut(s) 77, 155, 285
Bso31I GGTCTC 3 cut(s) 77, 155, 285
Bsp1286I GDGCHC 2 cut(s) 9, 364
Bsp143I GATC 2 cut(s) 123, 219
BspACI CCGC 1 cut(s) 81
BspPI GGATC 1 cut(s) 214
BspTNI GGTCTC 3 cut(s) 77, 155, 285
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 30
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 2 cut(s) 123, 219
Bst4CI ACNGT 3 cut(s) 166, 193, 296
BstC8I GCNNGC 1 cut(s) 32
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 3 cut(s) 205, 319, 333
BstKTI GATC 2 cut(s) 126, 222
BstMAI GTCTC 3 cut(s) 77, 155, 285
BstMBI GATC 2 cut(s) 123, 219
BstNSI RCATGY 1 cut(s) 378
BstX2I RGATCY 1 cut(s) 219
BstYI RGATCY 1 cut(s) 219
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 3 cut(s) 205, 319, 333
Cac8I GCNNGC 1 cut(s) 32
Cfr10I RCCGGY 1 cut(s) 30
CviAII CATG 1 cut(s) 375
CviJI RGCY 7 cut(s) 7, 22, 30, 34, 109, 115, 186
CviKI_1 RGCY 7 cut(s) 7, 22, 30, 34, 109, 115, 186
DpnI GATC 2 cut(s) 125, 221
DpnII GATC 2 cut(s) 123, 219
Eco24I GRGCYC 1 cut(s) 9
Eco31I GGTCTC 3 cut(s) 77, 155, 285
EcoRI GAATTC 1 cut(s) 57
EcoT38I GRGCYC 1 cut(s) 9
FaeI CATG 1 cut(s) 378
FaiI YATR 1 cut(s) 376
FatI CATG 1 cut(s) 374
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 3 cut(s) 212, 326, 340
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 1 cut(s) 81
GluI GCNGC 1 cut(s) 81
HapII CCGG 1 cut(s) 31
Hin1II CATG 1 cut(s) 378
HindIII AAGCTT 1 cut(s) 20
HpaII CCGG 1 cut(s) 31
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 2 cut(s) 15, 277
Hpy188I TCNGA 2 cut(s) 63, 224
Hpy188III TCNNGA 4 cut(s) 16, 74, 212, 247
Hpy8I GTNNAC 2 cut(s) 15, 277
HpyCH4III ACNGT 3 cut(s) 166, 193, 296
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 3 cut(s) 322, 356, 397
HpySE526I ACGT 1 cut(s) 11
Hsp92II CATG 1 cut(s) 378
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Kzo9I GATC 2 cut(s) 123, 219
LpnPI CCDG 7 cut(s) 44, 69, 181, 197, 242, 260, 310
LweI GCATC 2 cut(s) 42, 67
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 1 cut(s) 127
MalI GATC 2 cut(s) 125, 221
MboI GATC 2 cut(s) 123, 219
MflI RGATCY 1 cut(s) 219
MhlI GDGCHC 2 cut(s) 9, 364
MluCI AATT 3 cut(s) 57, 135, 317
MmeI TCCRAC 1 cut(s) 247
MnlI CCTC 3 cut(s) 228, 298, 331
MroNI GCCGGC 1 cut(s) 30
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 2 cut(s) 153, 381
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 1 cut(s) 31
NaeI GCCGGC 1 cut(s) 32
NdeII GATC 2 cut(s) 123, 219
NgoMIV GCCGGC 1 cut(s) 30
NlaIII CATG 1 cut(s) 378
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 378
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdiI GCCGGC 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 23
PkrI GCNGC 1 cut(s) 82
PsuI RGATCY 1 cut(s) 219
SaqAI TTAA 2 cut(s) 153, 381
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 2 cut(s) 123, 219
SduI GDGCHC 2 cut(s) 9, 364
SetI ASST 7 cut(s) 14, 24, 111, 117, 188, 229, 342
SfaNI GCATC 2 cut(s) 42, 67
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 3 cut(s) 57, 135, 317
SsiI CCGC 1 cut(s) 81
TaaI ACNGT 3 cut(s) 166, 193, 296
TaiI ACGT 1 cut(s) 14
TaqI TCGA 1 cut(s) 75
TaqII GACCGA 1 cut(s) 59
TasI AATT 3 cut(s) 57, 135, 317
TauI GCSGC 1 cut(s) 83
Tru1I TTAA 2 cut(s) 153, 381
Tru9I TTAA 2 cut(s) 153, 381
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 330
XapI RAATTY 1 cut(s) 57
XceI RCATGY 1 cut(s) 378
XmnI GAANNNNTTC 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.