Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0017201

Physical Location & Seq

Genomic Coordinates Reverse (-)

4228692 .. 4230686

Gene Structure 1995 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1062000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1995 bp

ATGAGCGCCCCAGCTCAAGCTTTTACCAAAGCCAAGCACGTCGTCAGTGCCGCCGCCGCTGCAGCCACTCGATCCGTCGCCAAAACTAAGAATTTTCATGGCCACCTCATCACATCAGGCTCAGTCTCGCCGCAAGTCCGCTCAAATCTTGCAAAGTCTTACGCTTTATGCAGTCACGTCCCGTACGCGCGCAAACTGTTCGACGAATTGCCTGACCGGAGCTCATTTTTGTACACTTCGCTGATGCGGATGTATGTATGCAATGGCTTACCCTATGAGGCACTAGACGTGTTTGCGCAGATGGTGGCCTTAGGGAAGTGTTGCCCAGATAGGTTTACTTATCCGGTTGTGATTAAGGCTTGTGGTGATTTGTCCTTGATTGATGTTGGTGTTGTCGTTCACGGAAGAACGGTAGTTTGTGGGTTCAATTTAGATGGGTTTGTTCAGAACTCTTTGGTGGCAATGTATATGAGTTGTGGGGAGACGGAATTGGCAAAAAGGGTTTTTGATGCAATGCGAGAACGAAGTGTGGTGTCTTGGAATACCATGATCAGTGGGTCCTTTCGGAATGGGTGTGCCAAGGAAGCTTTGGGTGTTTTTTATTGGATGATGAATGTAGGTGTGGAGCCGGATTGCGCCACTGTGGTTTCTGTGTTGCCGGCTGTTGGTTCCTTGAAGGAGATAGTGTTGGGAAGACAAGTTCATGCCTTTGTAGAAGAGAAGGGTTTGGGGAAAATGATAAAGCTGAGAAATGCATTGGTGGATATGTATGTGAAGTGTGGTAGCATGAATGAGGCACGATTAGTTTTCGATAGCATGGGTGAGAGAGACGTCGTGACTTGGACGAGTATGATCAATGGATACATTTTGAATGGTGATGCAAGAAGTGCATTGGCACTTTGTTGGCTGATGCAGCGGGAAGGTGTAAAACCCAATTTCGTAACTATAGCTTCTCTTCTTTCAGCCTGTGGGAGCTTGCATCTATCAAAGCATGGACGGTGCTTGCACGCATGGGCTATTAGGCAAAAGCTTGAATCTGATGTTATGGTGGAGACCGCTTTAATTGACATGTACTCAAAATGCAATTGTGTTCATTGCAGCTTTCAAGTGTTTGCAAAGACTTCAAAAAAGAGAACAGTTCCCTGGAATGCAATTATCTCCGGCTGCATTCATAATCGGCTTGGTAGAGCAGCGATCGAACATTTCAAACAGATGCTCATGGAAGCAGTACAACCCAACGAAGCAACCTTGAACAGTCTGCTTCCTGCATATACCATTCTCGTTGATTTGCATCAAGCAATGAATATACATGGTTATGTAGTACGATCTGGGTTTCTTTCAAGTATTGAAGTTGCTACTGGTTTGATTGATTCATATTCGAAGTGTGGAAGTTTAGCGTATGCTCACCAAATTTTTAATGAAATCCCAGAAAAGGAGAGAGATATTATCCTGTGGAGTGTGATAATAGCTGGTTATGGAATGCACGGCCATGGCGAGGTTGCAGTTTCACTTTTCTATCATATGGTTCGATCTGGGGTGAGGCCAAATGATGTCACTTTTACCTCTGTTTTGCATGCTTGCAGCCATGCCGGTTTGGTGGACGAGGGCTTGGGGTTGTTCAGGTTCATGCTTGAAGACCCAAAAGTGAGTCCTCAAACTGATCACTACACGTGCATTGTGGATCTTCTTGGTCGCGCTGGTCGGTTGGCAGAAGCTTATGATCTGATTAGATCAATGCCGTTTCAGCCTAACCATGCCATTTGGGGTGCACTACTCGGTGCCTGTGTGATACATGAGAACGTTGAGCTGGGAGAGTTGGCCGTTAAGTGGCTTATTGAGCTCGAGCCAGAAAATACAGGAAACTACGTGTTGATGGCCAAAATCTATGCTGCAGTGGGAAGGTGGAAAGATGCAGAAAATGTGAGACATATGATGAATGAAAGCGGGTTACGAAAAACACCCGCTCACAGTCTGGTTGAGGTCAGAAATATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cofilin_ADF PPR PPR_2

Protein Analysis

665

Amino Acids

73.25

Weight (kDa)

8.14

Isoelectric Point (pI)

38.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	74 - 121	5.2e-06	PPR repeat family
PPR_2	PF13041	176 - 218	3.8e-08	PPR repeat family
PPR_2	PF13041	276 - 323	3.4e-09	PPR repeat family
PPR_3	PF13812	276 - 324	8.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	379 - 423	5.7e-07	PPR repeat family
PPR_2	PF13041	481 - 528	1.2e-10	PPR repeat family
E_motif	PF20431	598 - 660	1e-19	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0017201)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G39350
fragaria_vesca	FvH4_6g47350
malus_domestica	MD09G1062000.v1.1
prunus_persica	Prupe.3G258100_v2.0.a1
pyrus_communis	pycom111g05230
rosa_chinensis	RchiOBHm_Chr2g0166381
rosa_laevigata	RLG00000021616
rosa_multiflora	Rmu_sc0003016.1_g000010
rosa_roxburghii	Rroxscaffold_2G00084920
rosa_rugosa	Rorug02G0522600
rosa_samantha	Rh2BG600800 Rh2CG571500
rosa_wichuraiana	Rw2G049070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	834
Acc16I	TGCGCA	1 cut(s)	297
AccB1I	GGYRCC	1 cut(s)	1778
AccBSI	CCGCTC	2 cut(s)	141, 1964
AccII	CGCG	3 cut(s)	188, 190, 1695
AclI	AACGTT	1 cut(s)	1800
AclWI	GGATC	2 cut(s)	66, 1689
AcoI	YGGCCR	4 cut(s)	100, 1486, 1818, 1875
AcsI	RAATTY	2 cut(s)	91, 1410
AcvI	CACGTG	1 cut(s)	1671
AcyI	GRCGYC	1 cut(s)	831
AfaI	GTAC	5 cut(s)	185, 233, 1073, 1230, 1323
AfiI	CCNNNNNNNGG	4 cut(s)	665, 1432, 1495, 1827
AflIII	ACRYGT	4 cut(s)	288, 1068, 1668, 1866
AjiI	CACGTC	3 cut(s)	40, 178, 289
AjnI	CCWGG	1 cut(s)	1142
AloI	GAACNNNNNNTCC	2 cut(s)	684, 716
Alw21I	GWGCWC	3 cut(s)	224, 1771, 1842
Alw26I	GTCTC	5 cut(s)	130, 476, 822, 1046, 1918
Alw44I	GTGCAC	1 cut(s)	1767
AlwI	GGATC	2 cut(s)	66, 1689
Ama87I	CYCGRG	1 cut(s)	1841
AoxI	GGCC	6 cut(s)	100, 306, 1486, 1541, 1818, 1875
ApaLI	GTGCAC	1 cut(s)	1767
ApeKI	GCWGC	8 cut(s)	59, 62, 913, 1098, 1164, 1190, 1581, 1889
ApoI	RAATTY	2 cut(s)	91, 1410
AspLEI	GCGC	6 cut(s)	8, 190, 192, 298, 638, 1697
AspS9I	GGNCC	1 cut(s)	558
AsuHPI	GGTGA	5 cut(s)	377, 833, 887, 1397, 1549
AsuII	TTCGAA	1 cut(s)	1379
AvaI	CYCGRG	1 cut(s)	1841
AvaII	GGWCC	1 cut(s)	558
AxyI	CCTNAGG	1 cut(s)	310
BaeGI	GKGCMC	1 cut(s)	1771
BalI	TGGCCA	2 cut(s)	102, 1877
BanI	GGYRCC	1 cut(s)	1778
BanII	GRGCYC	2 cut(s)	224, 1842
BbrPI	CACGTG	1 cut(s)	1671
BbsI	GAAGAC	2 cut(s)	700, 1641
Bbv12I	GWGCWC	3 cut(s)	224, 1771, 1842
BbvI	GCAGC	8 cut(s)	46, 74, 925, 1110, 1151, 1202, 1593, 1876
BccI	CCATC	3 cut(s)	295, 428, 1867
BceAI	ACGGC	3 cut(s)	1501, 1723, 1805
BcgI	CGANNNNNNTGC	2 cut(s)	181, 215
BciT130I	CCWGG	1 cut(s)	1144
BciVI	GTATCC	1 cut(s)	854
BclI	TGATCA	3 cut(s)	549, 852, 1660
BcoDI	GTCTC	5 cut(s)	130, 476, 822, 1046, 1918
BfaI	CTAG	1 cut(s)	284
BfmI	CTRYAG	3 cut(s)	60, 945, 1890
BfoI	RGCGCY	1 cut(s)	9
BfuI	GTATCC	1 cut(s)	854
Bme1390I	CCNGG	1 cut(s)	1144
Bme18I	GGWCC	1 cut(s)	558
BmeT110I	CYCGRG	1 cut(s)	1841
BmgBI	CACGTC	3 cut(s)	40, 178, 289
BmgT120I	GGNCC	1 cut(s)	558
BmiI	GGNNCC	4 cut(s)	559, 627, 670, 1780
BmrFI	CCNGG	1 cut(s)	1144
BmsI	GCATC	8 cut(s)	234, 499, 868, 900, 988, 1203, 1300, 1900
BpiI	GAAGAC	2 cut(s)	700, 1641
Bpu14I	TTCGAA	1 cut(s)	1379
BsaAI	YACGTR	2 cut(s)	1671, 1867
BsaBI	GATNNNNATC	1 cut(s)	1290
BsaHI	GRCGYC	1 cut(s)	831
BsaI	GGTCTC	1 cut(s)	1046
BsaJI	CCNNGG	3 cut(s)	579, 1142, 1489
BsaWI	WCCGGW	2 cut(s)	216, 343
BsaXI	ACNNNNNCTCC	2 cut(s)	671, 701
Bsc4I	CCNNNNNNNGG	4 cut(s)	665, 1432, 1495, 1827
Bse118I	RCCGGY	2 cut(s)	658, 1589
Bse1I	ACTGG	1 cut(s)	1363
Bse21I	CCTNAGG	1 cut(s)	310
Bse3DI	GCAATG	5 cut(s)	268, 468, 519, 1093, 1305
Bse8I	GATNNNNATC	1 cut(s)	1290
BseBI	CCWGG	1 cut(s)	1144
BseDI	CCNNGG	3 cut(s)	579, 1142, 1489
BseGI	GGATG	2 cut(s)	255, 612
BseJI	GATNNNNATC	1 cut(s)	1290
BseLI	CCNNNNNNNGG	4 cut(s)	665, 1432, 1495, 1827
BseMI	GCAATG	5 cut(s)	268, 468, 519, 1093, 1305
BseMII	CTCAG	2 cut(s)	135, 737
BseNI	ACTGG	1 cut(s)	1363
BsePI	GCGCGC	1 cut(s)	188
BseSI	GKGCMC	1 cut(s)	1771
BseXI	GCAGC	8 cut(s)	46, 74, 925, 1110, 1151, 1202, 1593, 1876
BseYI	CCCAGC	2 cut(s)	10, 1807
Bsh1236I	CGCG	3 cut(s)	188, 190, 1695
Bsh1285I	CGRYCG	1 cut(s)	1197
BshFI	GGCC	6 cut(s)	102, 308, 1488, 1543, 1820, 1877
BshNI	GGYRCC	1 cut(s)	1778
BsiEI	CGRYCG	1 cut(s)	1197
BsiHKAI	GWGCWC	3 cut(s)	224, 1771, 1842
BsiHKCI	CYCGRG	1 cut(s)	1841
BsiSI	CCGG	6 cut(s)	217, 344, 629, 659, 1161, 1590
BsiWI	CGTACG	1 cut(s)	183
BslFI	GGGAC	1 cut(s)	164
BslI	CCNNNNNNNGG	4 cut(s)	665, 1432, 1495, 1827
BsmAI	GTCTC	5 cut(s)	130, 476, 822, 1046, 1918
BsmBI	CGTCTC	2 cut(s)	476, 822
BsmFI	GGGAC	1 cut(s)	164
BsmI	GAATGC	3 cut(s)	1153, 1167, 1485
BsnI	GGCC	6 cut(s)	102, 308, 1488, 1543, 1820, 1877
Bso31I	GGTCTC	1 cut(s)	1046
BsoBI	CYCGRG	1 cut(s)	1841
Bsp119I	TTCGAA	1 cut(s)	1379
Bsp1286I	GDGCHC	3 cut(s)	224, 1771, 1842
Bsp1407I	TGTACA	1 cut(s)	231
Bsp19I	CCATGG	1 cut(s)	1489
BspANI	GGCC	6 cut(s)	102, 308, 1488, 1543, 1820, 1877
BspCNI	CTCAG	2 cut(s)	134, 738
BspFNI	CGCG	3 cut(s)	188, 190, 1695
BspLI	GGNNCC	4 cut(s)	559, 627, 670, 1780
BspMAI	CTGCAG	2 cut(s)	64, 1894
BspPI	GGATC	2 cut(s)	66, 1689
BspT104I	TTCGAA	1 cut(s)	1379
BspT107I	GGYRCC	1 cut(s)	1778
BspTNI	GGTCTC	1 cut(s)	1046
BsrBI	CCGCTC	2 cut(s)	141, 1964
BsrDI	GCAATG	5 cut(s)	268, 468, 519, 1093, 1305
BsrFI	RCCGGY	2 cut(s)	658, 1589
BsrGI	TGTACA	1 cut(s)	231
BsrI	ACTGG	1 cut(s)	1363
BssAI	RCCGGY	2 cut(s)	658, 1589
BssECI	CCNNGG	3 cut(s)	579, 1142, 1489
BssHII	GCGCGC	1 cut(s)	188
BssNI	GRCGYC	1 cut(s)	831
BssT1I	CCWWGG	2 cut(s)	579, 1489
Bst2UI	CCWGG	1 cut(s)	1144
Bst4CI	ACNGT	7 cut(s)	198, 412, 643, 999, 1138, 1256, 1970
Bst6I	CTCTTC	2 cut(s)	711, 960
BstACI	GRCGYC	1 cut(s)	831
BstAPI	GCANNNNNTGC	1 cut(s)	887
BstAUI	TGTACA	1 cut(s)	231
BstBAI	YACGTR	2 cut(s)	1671, 1867
BstBI	TTCGAA	1 cut(s)	1379
BstC8I	GCNNGC	7 cut(s)	190, 660, 977, 1004, 1008, 1575, 1579
BstDEI	CTNAG	4 cut(s)	87, 121, 310, 746
BstDSI	CCRYGG	1 cut(s)	1489
BstF5I	GGATG	2 cut(s)	255, 612
BstFNI	CGCG	3 cut(s)	188, 190, 1695
BstH2I	RGCGCY	1 cut(s)	9
BstHHI	GCGC	6 cut(s)	8, 190, 192, 298, 638, 1697
BstMAI	GTCTC	5 cut(s)	130, 476, 822, 1046, 1918
BstMCI	CGRYCG	1 cut(s)	1197
BstMWI	GCNNNNNNNGC	8 cut(s)	56, 59, 62, 584, 887, 913, 1744, 1837
BstNI	CCWGG	1 cut(s)	1144
BstNSI	RCATGY	2 cut(s)	1072, 1577
BstSCI	CCNGG	1 cut(s)	1142
BstSFI	CTRYAG	3 cut(s)	60, 945, 1890
BstSLI	GKGCMC	1 cut(s)	1771
BstUI	CGCG	3 cut(s)	188, 190, 1695
BstV1I	GCAGC	8 cut(s)	46, 74, 925, 1110, 1151, 1202, 1593, 1876
BstV2I	GAAGAC	2 cut(s)	700, 1641
BstX2I	RGATCY	1 cut(s)	1681
BstYI	RGATCY	1 cut(s)	1681
Bsu36I	CCTNAGG	1 cut(s)	310
BsuI	GTATCC	1 cut(s)	854
BsuRI	GGCC	6 cut(s)	102, 308, 1488, 1543, 1820, 1877
BtgI	CCRYGG	1 cut(s)	1489
BtrI	CACGTC	3 cut(s)	40, 178, 289
BtsCI	GGATG	2 cut(s)	255, 612
BtsI	GCAGTG	1 cut(s)	1899
BtsIMutI	CAGTG	4 cut(s)	52, 559, 639, 1899
Cac8I	GCNNGC	7 cut(s)	190, 660, 977, 1004, 1008, 1575, 1579
CfoI	GCGC	6 cut(s)	8, 190, 192, 298, 638, 1697
Cfr10I	RCCGGY	2 cut(s)	658, 1589
Cfr13I	GGNCC	1 cut(s)	558
Csp6I	GTAC	5 cut(s)	184, 232, 1072, 1229, 1322
CviQI	GTAC	5 cut(s)	184, 232, 1072, 1229, 1322
DdeI	CTNAG	4 cut(s)	87, 121, 310, 746
EaeI	YGGCCR	4 cut(s)	100, 1486, 1818, 1875
Eam1104I	CTCTTC	2 cut(s)	711, 960
EarI	CTCTTC	2 cut(s)	711, 960
Ecl136II	GAGCTC	2 cut(s)	222, 1840
Eco130I	CCWWGG	2 cut(s)	579, 1489
Eco24I	GRGCYC	2 cut(s)	224, 1842
Eco31I	GGTCTC	1 cut(s)	1046
Eco47I	GGWCC	1 cut(s)	558
Eco53kI	GAGCTC	2 cut(s)	222, 1840
Eco72I	CACGTG	1 cut(s)	1671
Eco81I	CCTNAGG	1 cut(s)	310
Eco88I	CYCGRG	1 cut(s)	1841
EcoICRI	GAGCTC	2 cut(s)	222, 1840
EcoO109I	RGGNCCY	1 cut(s)	558
EcoRII	CCWGG	1 cut(s)	1142
EcoT14I	CCWWGG	2 cut(s)	579, 1489
EcoT22I	ATGCAT	1 cut(s)	757
EcoT38I	GRGCYC	2 cut(s)	224, 1842
ErhI	CCWWGG	2 cut(s)	579, 1489
Esp3I	CGTCTC	2 cut(s)	476, 822
FaqI	GGGAC	1 cut(s)	164
FauI	CCCGC	3 cut(s)	909, 1937, 1969
FauNDI	CATATG	2 cut(s)	1521, 1929
FbaI	TGATCA	3 cut(s)	549, 852, 1660
FokI	GGATG	2 cut(s)	262, 619
FriOI	GRGCYC	2 cut(s)	224, 1842
FspBI	CTAG	1 cut(s)	284
FspI	TGCGCA	1 cut(s)	297
GlaI	GCGC	6 cut(s)	7, 189, 191, 297, 637, 1696
GsaI	CCCAGC	2 cut(s)	14, 1811
HaeII	RGCGCY	1 cut(s)	9
HaeIII	GGCC	6 cut(s)	102, 308, 1488, 1543, 1820, 1877
HapII	CCGG	6 cut(s)	217, 344, 629, 659, 1161, 1590
HhaI	GCGC	6 cut(s)	8, 190, 192, 298, 638, 1697
Hin1I	GRCGYC	1 cut(s)	831
Hin6I	GCGC	6 cut(s)	6, 188, 190, 296, 636, 1695
HinP1I	GCGC	6 cut(s)	6, 188, 190, 296, 636, 1695
HindIII	AAGCTT	4 cut(s)	18, 585, 1028, 1713
HinfI	GANTC	3 cut(s)	1034, 1370, 1648
HpaII	CCGG	6 cut(s)	217, 344, 629, 659, 1161, 1590
HphI	GGTGA	5 cut(s)	377, 833, 887, 1397, 1549
Hpy166II	GTNNAC	5 cut(s)	234, 336, 400, 1600, 1769
Hpy188I	TCNGA	5 cut(s)	447, 567, 1039, 1725, 1985
Hpy188III	TCNNGA	1 cut(s)	835
Hpy8I	GTNNAC	5 cut(s)	234, 336, 400, 1600, 1769
Hpy99I	CGWCG	4 cut(s)	44, 80, 206, 836
HpyAV	CCTTC	4 cut(s)	670, 715, 914, 1893
HpyCH4III	ACNGT	7 cut(s)	198, 412, 643, 999, 1138, 1256, 1970
HpyCH4IV	ACGT	7 cut(s)	39, 177, 288, 831, 1670, 1800, 1866
HpyF10VI	GCNNNNNNNGC	8 cut(s)	56, 59, 62, 584, 887, 913, 1744, 1837
HpyF3I	CTNAG	4 cut(s)	87, 121, 310, 746
HpySE526I	ACGT	7 cut(s)	39, 177, 288, 831, 1670, 1800, 1866
Hsp92I	GRCGYC	1 cut(s)	831
HspAI	GCGC	6 cut(s)	6, 188, 190, 296, 636, 1695
KroI	GCCGGC	1 cut(s)	658
KroNI	GCCGGC	1 cut(s)	660
Ksp22I	TGATCA	3 cut(s)	549, 852, 1660
LmnI	GCTCC	3 cut(s)	219, 625, 972
Lsp1109I	GCAGC	8 cut(s)	46, 74, 925, 1110, 1151, 1202, 1593, 1876
LweI	GCATC	8 cut(s)	234, 499, 868, 900, 988, 1203, 1300, 1900
MaeI	CTAG	1 cut(s)	284
MaeII	ACGT	7 cut(s)	39, 177, 288, 831, 1670, 1800, 1866
MaeIII	GTNAC	5 cut(s)	173, 835, 940, 1552, 1947
MbiI	CCGCTC	2 cut(s)	141, 1964
MboII	GAAGA	6 cut(s)	417, 705, 728, 947, 1646, 1676
MfeI	CAATTG	1 cut(s)	1084
MflI	RGATCY	1 cut(s)	1681
MhlI	GDGCHC	3 cut(s)	224, 1771, 1842
MlsI	TGGCCA	2 cut(s)	102, 1877
MluCI	AATT	9 cut(s)	91, 206, 427, 488, 934, 1062, 1084, 1152, 1410
MluNI	TGGCCA	2 cut(s)	102, 1877
MlyI	GAGTC	1 cut(s)	1657
MnlI	CCTC	9 cut(s)	116, 271, 787, 1489, 1533, 1573, 1597, 1662, 1972
Mox20I	TGGCCA	2 cut(s)	102, 1877
Mph1103I	ATGCAT	1 cut(s)	757
MroNI	GCCGGC	1 cut(s)	658
MscI	TGGCCA	2 cut(s)	102, 1877
MseI	TTAA	4 cut(s)	354, 1061, 1416, 1824
MslI	CAYNNNNRTG	2 cut(s)	1314, 1488
Msp20I	TGGCCA	2 cut(s)	102, 1877
MspA1I	CMGCKG	2 cut(s)	59, 916
MspI	CCGG	6 cut(s)	217, 344, 629, 659, 1161, 1590
MspR9I	CCNGG	1 cut(s)	1144
MunI	CAATTG	1 cut(s)	1084
Mva1269I	GAATGC	3 cut(s)	1153, 1167, 1485
MvaI	CCWGG	1 cut(s)	1144
MvnI	CGCG	3 cut(s)	188, 190, 1695
MwoI	GCNNNNNNNGC	8 cut(s)	56, 59, 62, 584, 887, 913, 1744, 1837
NaeI	GCCGGC	1 cut(s)	660
NcoI	CCATGG	1 cut(s)	1489
NdeI	CATATG	2 cut(s)	1521, 1929
NgoMIV	GCCGGC	1 cut(s)	658
NlaIV	GGNNCC	4 cut(s)	559, 627, 670, 1780
NmuCI	GTSAC	3 cut(s)	173, 835, 1552
NsbI	TGCGCA	1 cut(s)	297
NsiI	ATGCAT	1 cut(s)	757
NspI	RCATGY	2 cut(s)	1072, 1577
NspV	TTCGAA	1 cut(s)	1379
PaeI	GCATGC	1 cut(s)	1577
PaeR7I	CTCGAG	1 cut(s)	1841
PauI	GCGCGC	1 cut(s)	188
PciI	ACATGT	1 cut(s)	1068
PcsI	WCGNNNNNNNCGW	1 cut(s)	1491
PctI	GAATGC	3 cut(s)	1153, 1167, 1485
PdiI	GCCGGC	1 cut(s)	660
PfeI	GAWTC	2 cut(s)	1034, 1370
Pfl23II	CGTACG	1 cut(s)	183
Ple19I	CGATCG	1 cut(s)	1197
PleI	GAGTC	1 cut(s)	1656
PmaCI	CACGTG	1 cut(s)	1671
PmlI	CACGTG	1 cut(s)	1671
PpsI	GAGTC	1 cut(s)	1656
Ppu21I	YACGTR	2 cut(s)	1671, 1867
PpuMI	RGGWCCY	1 cut(s)	558
PscI	ACATGT	1 cut(s)	1068
Psp124BI	GAGCTC	2 cut(s)	224, 1842
Psp1406I	AACGTT	1 cut(s)	1800
Psp5II	RGGWCCY	1 cut(s)	558
Psp6I	CCWGG	1 cut(s)	1142
PspCI	CACGTG	1 cut(s)	1671
PspFI	CCCAGC	2 cut(s)	10, 1807
PspGI	CCWGG	1 cut(s)	1142
PspLI	CGTACG	1 cut(s)	183
PspN4I	GGNNCC	4 cut(s)	559, 627, 670, 1780
PspPI	GGNCC	1 cut(s)	558
PspPPI	RGGWCCY	1 cut(s)	558
PspXI	VCTCGAGB	1 cut(s)	1841
PstI	CTGCAG	2 cut(s)	64, 1894
PsuI	RGATCY	1 cut(s)	1681
PteI	GCGCGC	1 cut(s)	188
PvuI	CGATCG	1 cut(s)	1197
RsaI	GTAC	5 cut(s)	185, 233, 1073, 1230, 1323
RsaNI	GTAC	5 cut(s)	184, 232, 1072, 1229, 1322
RseI	CAYNNNNRTG	2 cut(s)	1314, 1488
SacI	GAGCTC	2 cut(s)	224, 1842
SaqAI	TTAA	4 cut(s)	354, 1061, 1416, 1824
Sau96I	GGNCC	1 cut(s)	558
SchI	GAGTC	1 cut(s)	1657
ScrFI	CCNGG	1 cut(s)	1144
SduI	GDGCHC	3 cut(s)	224, 1771, 1842
SfaNI	GCATC	8 cut(s)	234, 499, 868, 900, 988, 1203, 1300, 1900
SfcI	CTRYAG	3 cut(s)	60, 945, 1890
Sfr274I	CTCGAG	1 cut(s)	1841
SfuI	TTCGAA	1 cut(s)	1379
SinI	GGWCC	1 cut(s)	558
SlaI	CTCGAG	1 cut(s)	1841
SmiMI	CAYNNNNRTG	2 cut(s)	1314, 1488
SmlI	CTYRAG	2 cut(s)	15, 1841
SmoI	CTYRAG	2 cut(s)	15, 1841
SphI	GCATGC	1 cut(s)	1577
Sse9I	AATT	9 cut(s)	91, 206, 427, 488, 934, 1062, 1084, 1152, 1410
SspMI	CTAG	1 cut(s)	284
SstI	GAGCTC	2 cut(s)	224, 1842
StyD4I	CCNGG	1 cut(s)	1142
StyI	CCWWGG	2 cut(s)	579, 1489
TaaI	ACNGT	7 cut(s)	198, 412, 643, 999, 1138, 1256, 1970
TaiI	ACGT	7 cut(s)	42, 180, 291, 834, 1673, 1803, 1869
TaqI	TCGA	7 cut(s)	70, 201, 810, 1197, 1379, 1528, 1842
TasI	AATT	9 cut(s)	91, 206, 427, 488, 934, 1062, 1084, 1152, 1410
TatI	WGTACW	3 cut(s)	231, 1071, 1228
TauI	GCSGC	4 cut(s)	53, 56, 59, 133
TfiI	GAWTC	2 cut(s)	1034, 1370
Tru1I	TTAA	4 cut(s)	354, 1061, 1416, 1824
Tru9I	TTAA	4 cut(s)	354, 1061, 1416, 1824
TscAI	CASTG	4 cut(s)	52, 559, 646, 1899
TseFI	GTSAC	3 cut(s)	173, 835, 1552
TseI	GCWGC	8 cut(s)	59, 62, 913, 1098, 1164, 1190, 1581, 1889
Tsp45I	GTSAC	3 cut(s)	173, 835, 1552
TspGWI	ACGGA	3 cut(s)	64, 417, 500
TspRI	CASTG	4 cut(s)	52, 559, 646, 1899
VneI	GTGCAC	1 cut(s)	1767
VpaK11BI	GGWCC	1 cut(s)	558
XapI	RAATTY	2 cut(s)	91, 1410
XceI	RCATGY	2 cut(s)	1072, 1577
XcmI	CCANNNNNNNNNTGG	1 cut(s)	586
XhoI	CTCGAG	1 cut(s)	1841
XspI	CTAG	1 cut(s)	284
ZraI	GACGTC	1 cut(s)	832
Zsp2I	ATGCAT	1 cut(s)	757

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.