MD09G1148300.v1.1

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009515
Physical Location & Seq
Forward (+)
11661752 .. 11668547
6796 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1148300.v1.1.491

Sequence Viewer

Length: 2763 bp
ATGGCGGGTATTGTCAAACCGCCGGGGGCTTTCGCGGTGACGCCGCACAAAGTCTCCGTCTGCATACTGCTCCAGATCTACGCGCCGCCCTCTCAAATCTCAGTCCCGTTCCCCTTCTCCACCGTCAGCCAGCACAACCGCCTCGGCCTGTTCTTGCTCTCCCTCACTAAGTCTTATGATGATATGTTCGAGCCGAAATTGGATGAACTTATACACCAATTGAGGGGAATTGGGGGATTATTGAACTATTGGTTAACCGATCATCTGACCAGTAGGTTGTCGGCTCTGTCCTCGCCGGATGATTTGTTTAACTTCTTTAGTGATATGCGAGGGATCCTTGGGGGCCCCGAAGCAGGTGTTTTGGAAGATGACCAAGTTATTCTGGATCCAAATAGCAACTTGGGGATGTTTCTCCGACGTTGCATACTTGCGTTTAATCTTTTATCCTTTGAGGGTGCGTGTCATCTTTTAACAAGTATAGGAATGTACTGTAAAGAAGCCATCACAAGTTGTCCTCCATATGAGTCACCTCACTTAGATGATTCAAGTAATGATTTAGAAACACCTCCGGAATATGAAAACATGGAACTGGAGAATCTTGTTTTTGAAAAAGTCACCGAAGAAATCGAAGCAAGAGAAAGAGCTGGTGGAAGAGTTTCTTTTCATCTTCATGCACCCAAAGCACTTGTTGGATTGGTTGAAGATATTGAAGTCCCTGGTGATCCAGAATTCAAACATGGTGGCAATAGAGAAGATTGTCATTATGCACATCCTACAAGCAGTACATTCAGTGATCTTGATCCCAACGGTGGGATATTCCTACGGACAAACTGGCAGATACAGGGATTTCTACAGGAGCAAGCTGATGCACTTGAAAAGCAGGGGAGCTCTTTCTCTTTAAATGCTTTTGAGCTCATGCTTAGGCAGCTTCAAAAACTGGCACCTGAGCTACATCGTGTTCACTTCTTGCGTTACTTGAATGGTCTGTATCATGATGATTTTTTTGCTGCCTTGGAGAATGTTCACCGCTATTTTGATTACAGCTCAGGGATTGAAGGATTTGATTTCGTTCCTCCTGCATCCGGTTGCAATAGCTTAGGAAGATATGAAATTGCCTTGCTTTGTTTAGGAATGATGCATTTCCATTTTGGGCACCCAAAGCAAGCTTTGGAGGTTTTGACCGAGGCAGTACATATTTCTCAACAGCAAAGTAACGATACATGTCTTGCTTACACCTTAGCGGCTATCTGCAATTTGTTGTCCGAAACTGGCATATCTAGTACAACTGGAATACTGGGCTCATCATATTCACCTTTGACCAGAATAGGCATTTCACTGTCTGTCCAGCAACAACTATTTGTTCTCTTGAGAGGGTCTCTGAAGAGAGCAGAAAATTTGAAGTTGAAGCGTTTAGTGGCATCCAATCATCTTGCGATGGCTAAATTTGATTTAACGCATGTGCAAAGGCCTCTAGTTTCATTTGGACCCAAGGCTTCAATGAAGCTTAGAACAAACCCAGTTACTGTTTGCAAGGAACTTAGATTGAGTTCTCAATTGATTAGTGAGTTTGGATTCGAAACTTCTTCAATGACAACTGATGGTGCTTTTAGTACTGCATGGCTTAAGAATTTACAGAAGCCAATGGATTCACAAGTCTTGTCTCAAGAAAGTGGAACAGGAAGCAATAATGCTTTCCAGTTCTGTGCACAACCAAGTTCAGTCCCTGCATCTGTGTTGCAGTTAATAGGCTCTTCATACTTACTTCGTGCCACTGCATGGGAAATATATGGCAGCTCCTCTCTTGCCAGATTTAATGCACTTGTTCATGCAACTTGTTTTCCTGATGTTTCAAGTTCATCTGACACAGCATTAGCATATCTGAAGCTCATCCAACATCTAGCCGTTTATAAAGGATACAAAGAGGCTTTTGCTGCTCTTAAAATAGCTGCAGAGAAGTTTTTATCCATCTCAAAATCAAGAATTTTGTTATTGAAACTTCAGCTGCTCCATGAGCGTGCTTTACATAGAGGACATTTGAAGTTTGCCCAACAAGTATGTGATGAGCTTGGAGTCCTGGCATCATCTGTCAATGGCGTGGATATGGAACTGAAGACGGAAGCAAGTCTCAGAAAAGCTCGTACATTGCTTGCCGCAAATCAGTTCAGTGAGGCGGCAGCTGTAGCACACTCACTCTTCTGCATGTGTTACAAATTCAATATGCAAGTTGAAAATGCCAGTGTGCTTCTTTTGCTAGCTGAAATCCACAAGAAATCGGGTAATGCTGTTTTAGGTCTTCCGTATGCATTGGCAAGCCTCTCATTTTGCCAGTCGTTTAATCTGGATCTGCTTAAAGCATCGGCCACACTCACACTAGCTGAGTTGTGGCTCTCACTTGGATCAAGTCATGCAAAAAGGGCCCTGAGCCTTGTACATGGGGCTTTTCCTATGATTCTTGGTCAAGGAGGTTTGGAGCTCCGTGCTCGGGCCTTCATTGTAGAAGCAAAATGCTATCTATCCGATCCAAGCTTTTCCATTTCTGAAAGCTCCGATGATGTGCTGGATCCGCTGAGACAAGCCTCGGACGAGCTTCAATTGCTAGAGTATCACGAATTGGCGGCCGAAGCTTTCTATTTAATGGCTATGGTGTTCGACAAACTGAAAAGAGTAGAGGACAGAGAAGACGCTGCGGCTTCGTTTAAGCAACACACTTTGGCTCTTGAGAATCCGCAACATGAGGAAGATCCTCTTATCAACATGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko04657 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04657 map04914 map05166
Pfam Domains
Protein Families
ANAPC5

Protein Analysis

921

Amino Acids

101.61

Weight (kDa)

5.72

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC5 PF12862 321 - 422 4.6e-20 Anaphase-promoting complex subunit 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1908
AarI CACCTGC 1 cut(s) 344
Acc36I ACCTGC 1 cut(s) 344
AccB1I GGYRCC 2 cut(s) 940, 1152
AccB7I CCANNNNNTGG 1 cut(s) 1776
AccII CGCG 2 cut(s) 35, 83
AccIII TCCGGA 1 cut(s) 568
AcoI YGGCCR 2 cut(s) 2358, 2616
AcsI RAATTY 6 cut(s) 728, 1393, 1442, 1627, 1980, 2210
AcuI CTGAAG 4 cut(s) 1400, 1901, 1982, 2129
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 7 cut(s) 488, 784, 1191, 1282, 1612, 2140, 2430
AfiI CCNNNNNNNGG 7 cut(s) 223, 353, 809, 810, 1082, 1776, 2482
AflII CTTAAG 1 cut(s) 1622
AflIII ACRYGT 2 cut(s) 1220, 2754
AjnI CCWGG 2 cut(s) 715, 2073
Alw21I GWGCWC 5 cut(s) 890, 915, 1708, 2475, 2482
Alw26I GTCTC 5 cut(s) 58, 1380, 1665, 2129, 2563
Alw44I GTGCAC 1 cut(s) 1704
AlwNI CAGNNNCTG 2 cut(s) 1523, 1724
Ama87I CYCGRG 1 cut(s) 2481
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 7 cut(s) 145, 343, 1466, 2358, 2415, 2484, 2616
ApaI GGGCCC 2 cut(s) 347, 2419
ApaLI GTGCAC 1 cut(s) 1704
ApeKI GCWGC 8 cut(s) 925, 1007, 1791, 1931, 1946, 2002, 2174, 2684
ApoI RAATTY 6 cut(s) 728, 1393, 1442, 1627, 1980, 2210
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
Asp700I GAANNNNTTC 1 cut(s) 655
AspLEI GCGC 1 cut(s) 85
AspS9I GGNCC 6 cut(s) 343, 344, 1484, 2415, 2416, 2484
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 6 cut(s) 49, 519, 607, 731, 1016, 1302
AsuII TTCGAA 1 cut(s) 1575
AsuNHI GCTAGC 1 cut(s) 2251
AvaI CYCGRG 1 cut(s) 2481
AvaII GGWCC 1 cut(s) 1484
BaeGI GKGCMC 4 cut(s) 347, 1155, 1708, 2419
BamHI GGATCC 3 cut(s) 333, 385, 2560
BanI GGYRCC 2 cut(s) 940, 1152
BanII GRGCYC 6 cut(s) 347, 890, 915, 1301, 2419, 2475
BbsI GAAGAC 3 cut(s) 2117, 2285, 2685
Bbv12I GWGCWC 5 cut(s) 890, 915, 1708, 2475, 2482
BbvI GCAGC 8 cut(s) 937, 994, 1803, 1918, 1933, 1989, 2186, 2671
BccI CCATC 4 cut(s) 509, 1429, 1592, 1973
BceAI ACGGC 1 cut(s) 1886
BciT130I CCWGG 2 cut(s) 717, 2075
BciVI GTATCC 1 cut(s) 1907
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 5 cut(s) 58, 1380, 1665, 2129, 2563
BfaI CTAG 6 cut(s) 1278, 1472, 1898, 2252, 2372, 2597
BfmI CTRYAG 3 cut(s) 851, 1947, 2178
BfrI CTTAAG 1 cut(s) 1622
BfuAI ACCTGC 1 cut(s) 344
BfuI GTATCC 1 cut(s) 1907
BglII AGATCT 1 cut(s) 75
BmcAI AGTACT 1 cut(s) 1612
Bme1390I CCNGG 3 cut(s) 24, 717, 2075
Bme18I GGWCC 1 cut(s) 1484
BmeT110I CYCGRG 1 cut(s) 2481
BmgT120I GGNCC 6 cut(s) 343, 344, 1484, 2415, 2416, 2484
BmrFI CCNGG 3 cut(s) 24, 717, 2075
BmrI ACTGGG 2 cut(s) 1304, 1511
BmsI GCATC 7 cut(s) 856, 1088, 1125, 1427, 1736, 2087, 2363
BmtI GCTAGC 1 cut(s) 2255
BmuI ACTGGG 2 cut(s) 1304, 1511
BpiI GAAGAC 3 cut(s) 2117, 2285, 2685
BplI GAGNNNNNCTC 2 cut(s) 1360, 1392
BpmI CTGGAG 2 cut(s) 56, 611
Bpu10I CCTNAGC 6 cut(s) 920, 945, 1045, 1096, 1237, 2420
Bpu14I TTCGAA 1 cut(s) 1575
BpuEI CTTGAG 3 cut(s) 1387, 1647, 2738
BpuMI CCSGG 1 cut(s) 24
BsaBI GATNNNNATC 1 cut(s) 798
BsaHI GRCGYC 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 1380
BsaJI CCNNGG 8 cut(s) 23, 142, 337, 715, 1011, 1182, 1488, 2577
BsaWI WCCGGW 2 cut(s) 568, 1082
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 7 cut(s) 223, 353, 809, 810, 1082, 1776, 2482
Bse3DI GCAATG 1 cut(s) 2141
Bse8I GATNNNNATC 1 cut(s) 798
BseAI TCCGGA 1 cut(s) 568
BseBI CCWGG 2 cut(s) 717, 2075
BseDI CCNNGG 8 cut(s) 23, 142, 337, 715, 1011, 1182, 1488, 2577
BseGI GGATG 7 cut(s) 208, 304, 411, 769, 1079, 1418, 1887
BseJI GATNNNNATC 1 cut(s) 798
BseLI CCNNNNNNNGG 7 cut(s) 223, 353, 809, 810, 1082, 1776, 2482
BseMI GCAATG 1 cut(s) 2141
BseMII CTCAG 7 cut(s) 114, 936, 1059, 2140, 2367, 2411, 2558
BseRI GAGGAG 1 cut(s) 1786
BseSI GKGCMC 4 cut(s) 347, 1155, 1708, 2419
BseX3I CGGCCG 1 cut(s) 2616
BseXI GCAGC 8 cut(s) 937, 994, 1803, 1918, 1933, 1989, 2186, 2671
Bsh1236I CGCG 2 cut(s) 35, 83
Bsh1285I CGRYCG 1 cut(s) 2619
BshFI GGCC 7 cut(s) 147, 345, 1468, 2360, 2417, 2486, 2618
BshNI GGYRCC 2 cut(s) 940, 1152
BsiEI CGRYCG 1 cut(s) 2619
BsiHKAI GWGCWC 5 cut(s) 890, 915, 1708, 2475, 2482
BsiHKCI CYCGRG 1 cut(s) 2481
BsiSI CCGG 4 cut(s) 23, 296, 569, 1083
BslFI GGGAC 3 cut(s) 89, 698, 1706
BslI CCNNNNNNNGG 7 cut(s) 223, 353, 809, 810, 1082, 1776, 2482
BsmAI GTCTC 5 cut(s) 58, 1380, 1665, 2129, 2563
BsmFI GGGAC 3 cut(s) 89, 698, 1706
BsnI GGCC 7 cut(s) 147, 345, 1468, 2360, 2417, 2486, 2618
Bso31I GGTCTC 1 cut(s) 1380
BsoBI CYCGRG 1 cut(s) 2481
Bsp119I TTCGAA 1 cut(s) 1575
Bsp120I GGGCCC 2 cut(s) 343, 2415
Bsp1286I GDGCHC 9 cut(s) 347, 890, 915, 1155, 1301, 1708, 2419, 2475, 2482
Bsp13I TCCGGA 1 cut(s) 568
Bsp1407I TGTACA 1 cut(s) 2428
BspANI GGCC 7 cut(s) 147, 345, 1468, 2360, 2417, 2486, 2618
BspCNI CTCAG 7 cut(s) 113, 937, 1058, 2139, 2368, 2412, 2559
BspEI TCCGGA 1 cut(s) 568
BspFNI CGCG 2 cut(s) 35, 83
BspHI TCATGA 1 cut(s) 991
BspMAI CTGCAG 1 cut(s) 1951
BspMI ACCTGC 1 cut(s) 344
BspOI GCTAGC 1 cut(s) 2255
BspQI GCTCTTC 1 cut(s) 1756
BspT104I TTCGAA 1 cut(s) 1575
BspT107I GGYRCC 2 cut(s) 940, 1152
BspTI CTTAAG 1 cut(s) 1622
BspTNI GGTCTC 1 cut(s) 1380
BsrDI GCAATG 1 cut(s) 2141
BsrGI TGTACA 1 cut(s) 2428
BssECI CCNNGG 8 cut(s) 23, 142, 337, 715, 1011, 1182, 1488, 2577
BssNI GRCGYC 1 cut(s) 41
BssT1I CCWWGG 3 cut(s) 337, 1011, 1488
Bst2UI CCWGG 2 cut(s) 717, 2075
Bst4CI ACNGT 5 cut(s) 124, 491, 809, 1338, 1525
Bst6I CTCTTC 4 cut(s) 646, 1376, 1756, 2198
BstACI GRCGYC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 1622
BstAUI TGTACA 1 cut(s) 2428
BstBI TTCGAA 1 cut(s) 1575
BstC8I GCNNGC 7 cut(s) 131, 861, 1164, 2016, 2148, 2253, 2311
BstF5I GGATG 7 cut(s) 208, 304, 411, 769, 1079, 1418, 1887
BstFNI CGCG 2 cut(s) 35, 83
BstHHI GCGC 1 cut(s) 85
BstMAI GTCTC 5 cut(s) 58, 1380, 1665, 2129, 2563
BstMCI CGRYCG 1 cut(s) 2619
BstNI CCWGG 2 cut(s) 717, 2075
BstNSI RCATGY 4 cut(s) 1224, 1460, 2203, 2758
BstSCI CCNGG 3 cut(s) 22, 715, 2073
BstSFI CTRYAG 3 cut(s) 851, 1947, 2178
BstSLI GKGCMC 4 cut(s) 347, 1155, 1708, 2419
BstUI CGCG 2 cut(s) 35, 83
BstV1I GCAGC 8 cut(s) 937, 994, 1803, 1918, 1933, 1989, 2186, 2671
BstV2I GAAGAC 3 cut(s) 2117, 2285, 2685
BstX2I RGATCY 6 cut(s) 75, 333, 385, 2341, 2560, 2740
BstYI RGATCY 6 cut(s) 75, 333, 385, 2341, 2560, 2740
BstZI CGGCCG 1 cut(s) 2616
BsuI GTATCC 1 cut(s) 1907
BsuRI GGCC 7 cut(s) 147, 345, 1468, 2360, 2417, 2486, 2618
BtgZI GCGATG 1 cut(s) 1448
BtsCI GGATG 7 cut(s) 208, 304, 411, 769, 1079, 1418, 1887
BtsI GCAGTG 1 cut(s) 1770
BtsIMutI CAGTG 5 cut(s) 796, 1334, 1770, 2170, 2242
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 7 cut(s) 131, 861, 1164, 2016, 2148, 2253, 2311
CaiI CAGNNNCTG 2 cut(s) 1523, 1724
CciI TCATGA 1 cut(s) 991
CfoI GCGC 1 cut(s) 85
Cfr13I GGNCC 6 cut(s) 343, 344, 1484, 2415, 2416, 2484
CseI GACGC 2 cut(s) 49, 2690
Csp6I GTAC 7 cut(s) 487, 783, 1190, 1281, 1611, 2139, 2429
CspCI CAANNNNNGTGG 2 cut(s) 721, 756
CviQI GTAC 7 cut(s) 487, 783, 1190, 1281, 1611, 2139, 2429
DraI TTTAAA 1 cut(s) 900
EaeI YGGCCR 2 cut(s) 2358, 2616
EagI CGGCCG 1 cut(s) 2616
Eam1104I CTCTTC 4 cut(s) 646, 1376, 1756, 2198
EarI CTCTTC 4 cut(s) 646, 1376, 1756, 2198
Ecl136II GAGCTC 3 cut(s) 888, 913, 2473
EclXI CGGCCG 1 cut(s) 2616
Eco130I CCWWGG 3 cut(s) 337, 1011, 1488
Eco147I AGGCCT 1 cut(s) 1468
Eco24I GRGCYC 6 cut(s) 347, 890, 915, 1301, 2419, 2475
Eco31I GGTCTC 1 cut(s) 1380
Eco47I GGWCC 1 cut(s) 1484
Eco52I CGGCCG 1 cut(s) 2616
Eco53kI GAGCTC 3 cut(s) 888, 913, 2473
Eco57I CTGAAG 4 cut(s) 1400, 1901, 1982, 2129
Eco88I CYCGRG 1 cut(s) 2481
EcoICRI GAGCTC 3 cut(s) 888, 913, 2473
EcoO109I RGGNCCY 4 cut(s) 343, 344, 2415, 2416
EcoRI GAATTC 1 cut(s) 728
EcoRII CCWGG 2 cut(s) 715, 2073
EcoT14I CCWWGG 3 cut(s) 337, 1011, 1488
EcoT22I ATGCAT 2 cut(s) 1140, 2305
EcoT38I GRGCYC 6 cut(s) 347, 890, 915, 1301, 2419, 2475
ErhI CCWWGG 3 cut(s) 337, 1011, 1488
FalI AAGNNNNNCTT 4 cut(s) 643, 675, 2730, 2762
FaqI GGGAC 3 cut(s) 89, 698, 1706
FauNDI CATATG 1 cut(s) 520
FokI GGATG 7 cut(s) 215, 311, 418, 756, 1066, 1405, 1874
FriOI GRGCYC 6 cut(s) 347, 890, 915, 1301, 2419, 2475
FspBI CTAG 6 cut(s) 1278, 1472, 1898, 2252, 2372, 2597
GlaI GCGC 1 cut(s) 84
GsuI CTGGAG 2 cut(s) 56, 611
HaeIII GGCC 7 cut(s) 147, 345, 1468, 2360, 2417, 2486, 2618
HapII CCGG 4 cut(s) 23, 296, 569, 1083
HgaI GACGC 2 cut(s) 49, 2690
HhaI GCGC 1 cut(s) 85
Hin1I GRCGYC 1 cut(s) 41
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HindIII AAGCTT 4 cut(s) 1164, 1502, 2524, 2622
HinfI GANTC 8 cut(s) 524, 542, 595, 1572, 1646, 2070, 2449, 2722
HpaI GTTAAC 1 cut(s) 255
HpaII CCGG 4 cut(s) 23, 296, 569, 1083
HphI GGTGA 6 cut(s) 49, 519, 607, 731, 1016, 1302
Hpy166II GTNNAC 4 cut(s) 255, 961, 1024, 1706
Hpy8I GTNNAC 4 cut(s) 255, 961, 1024, 1706
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 3 cut(s) 124, 1049, 2497
HpyCH4III ACNGT 5 cut(s) 124, 491, 809, 1338, 1525
HpyCH4IV ACGT 1 cut(s) 418
HpySE526I ACGT 1 cut(s) 418
Hsp92I GRCGYC 1 cut(s) 41
HspAI GCGC 1 cut(s) 83
Kpn2I TCCGGA 1 cut(s) 568
KspAI GTTAAC 1 cut(s) 255
LguI GCTCTTC 1 cut(s) 1756
LmnI GCTCC 8 cut(s) 75, 856, 885, 1799, 2010, 2470, 2478, 2549
Lsp1109I GCAGC 8 cut(s) 937, 994, 1803, 1918, 1933, 1989, 2186, 2671
LweI GCATC 7 cut(s) 856, 1088, 1125, 1427, 1736, 2087, 2363
MaeI CTAG 6 cut(s) 1278, 1472, 1898, 2252, 2372, 2597
MaeII ACGT 1 cut(s) 418
MaeIII GTNAC 7 cut(s) 37, 525, 613, 971, 1211, 1519, 2204
MfeI CAATTG 3 cut(s) 218, 1553, 2591
MflI RGATCY 6 cut(s) 75, 333, 385, 2341, 2560, 2740
MhlI GDGCHC 9 cut(s) 347, 890, 915, 1155, 1301, 1708, 2419, 2475, 2482
MlyI GAGTC 2 cut(s) 533, 2079
MmeI TCCRAC 3 cut(s) 439, 670, 1915
Mph1103I ATGCAT 2 cut(s) 1140, 2305
MroI TCCGGA 1 cut(s) 568
MroXI GAANNNNTTC 1 cut(s) 655
MslI CAYNNNNRTG 3 cut(s) 537, 669, 2013
MspA1I CMGCKG 3 cut(s) 2002, 2177, 2566
MspCI CTTAAG 1 cut(s) 1622
MspI CCGG 4 cut(s) 23, 296, 569, 1083
MspR9I CCNGG 3 cut(s) 24, 717, 2075
MunI CAATTG 3 cut(s) 218, 1553, 2591
MvaI CCWGG 2 cut(s) 717, 2075
MvnI CGCG 2 cut(s) 35, 83
NciI CCSGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 520
NheI GCTAGC 1 cut(s) 2251
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 3 cut(s) 37, 525, 613
NsiI ATGCAT 2 cut(s) 1140, 2305
NspI RCATGY 4 cut(s) 1224, 1460, 2203, 2758
NspV TTCGAA 1 cut(s) 1575
PagI TCATGA 1 cut(s) 991
PaqCI CACCTGC 1 cut(s) 344
PceI AGGCCT 1 cut(s) 1468
PciI ACATGT 2 cut(s) 1220, 2754
PciSI GCTCTTC 1 cut(s) 1756
PdmI GAANNNNTTC 1 cut(s) 655
PfeI GAWTC 6 cut(s) 542, 595, 1572, 1646, 2449, 2722
PflMI CCANNNNNTGG 1 cut(s) 1776
PleI GAGTC 2 cut(s) 532, 2078
PpsI GAGTC 2 cut(s) 532, 2078
PscI ACATGT 2 cut(s) 1220, 2754
PsiI TTATAA 1 cut(s) 1908
Psp124BI GAGCTC 3 cut(s) 890, 915, 2475
Psp6I CCWGG 2 cut(s) 715, 2073
PspGI CCWGG 2 cut(s) 715, 2073
PspOMI GGGCCC 2 cut(s) 343, 2415
PspPI GGNCC 6 cut(s) 343, 344, 1484, 2415, 2416, 2484
PstI CTGCAG 1 cut(s) 1951
PstNI CAGNNNCTG 2 cut(s) 1523, 1724
PsuI RGATCY 6 cut(s) 75, 333, 385, 2341, 2560, 2740
PvuII CAGCTG 2 cut(s) 2002, 2177
RsaI GTAC 7 cut(s) 488, 784, 1191, 1282, 1612, 2140, 2430
RsaNI GTAC 7 cut(s) 487, 783, 1190, 1281, 1611, 2139, 2429
RseI CAYNNNNRTG 3 cut(s) 537, 669, 2013
SacI GAGCTC 3 cut(s) 890, 915, 2475
SapI GCTCTTC 1 cut(s) 1756
Sau96I GGNCC 6 cut(s) 343, 344, 1484, 2415, 2416, 2484
ScaI AGTACT 1 cut(s) 1612
SchI GAGTC 2 cut(s) 533, 2079
ScrFI CCNGG 3 cut(s) 24, 717, 2075
SduI GDGCHC 9 cut(s) 347, 890, 915, 1155, 1301, 1708, 2419, 2475, 2482
SfaNI GCATC 7 cut(s) 856, 1088, 1125, 1427, 1736, 2087, 2363
SfcI CTRYAG 3 cut(s) 851, 1947, 2178
SfuI TTCGAA 1 cut(s) 1575
SinI GGWCC 1 cut(s) 1484
SmiMI CAYNNNNRTG 3 cut(s) 537, 669, 2013
SmlI CTYRAG 4 cut(s) 1366, 1622, 1662, 2717
SmoI CTYRAG 4 cut(s) 1366, 1622, 1662, 2717
SseBI AGGCCT 1 cut(s) 1468
SspMI CTAG 6 cut(s) 1278, 1472, 1898, 2252, 2372, 2597
SstI GAGCTC 3 cut(s) 890, 915, 2475
StuI AGGCCT 1 cut(s) 1468
StyD4I CCNGG 3 cut(s) 22, 715, 2073
StyI CCWWGG 3 cut(s) 337, 1011, 1488
TaaI ACNGT 5 cut(s) 124, 491, 809, 1338, 1525
TaiI ACGT 1 cut(s) 421
TaqI TCGA 4 cut(s) 189, 627, 1575, 2649
TaqII GACCGA 1 cut(s) 1196
TatI WGTACW 6 cut(s) 486, 782, 1189, 1280, 1610, 2428
TauI GCSGC 7 cut(s) 46, 88, 1244, 2153, 2174, 2618, 2690
TfiI GAWTC 6 cut(s) 542, 595, 1572, 1646, 2449, 2722
TscAI CASTG 5 cut(s) 796, 1341, 1777, 2170, 2242
TseFI GTSAC 3 cut(s) 37, 525, 613
TseI GCWGC 8 cut(s) 925, 1007, 1791, 1931, 1946, 2002, 2174, 2684
Tsp45I GTSAC 3 cut(s) 37, 525, 613
TspGWI ACGGA 5 cut(s) 46, 838, 2129, 2286, 2465
TspRI CASTG 5 cut(s) 796, 1341, 1777, 2170, 2242
Van91I CCANNNNNTGG 1 cut(s) 1776
Vha464I CTTAAG 1 cut(s) 1622
VneI GTGCAC 1 cut(s) 1704
VpaK11BI GGWCC 1 cut(s) 1484
XapI RAATTY 6 cut(s) 728, 1393, 1442, 1627, 1980, 2210
XceI RCATGY 4 cut(s) 1224, 1460, 2203, 2758
XmnI GAANNNNTTC 1 cut(s) 655
XspI CTAG 6 cut(s) 1278, 1472, 1898, 2252, 2372, 2597
ZrmI AGTACT 1 cut(s) 1612
Zsp2I ATGCAT 2 cut(s) 1140, 2305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.