Rorug02G0431700

Anaphase-promoting complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009515
Physical Location & Seq
Forward (+)
55334979 .. 55335362
384 bp
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UTR
Exon/CDS
Intron
Rorug02G0431700.1

Sequence Viewer

Length: 384 bp
ATGGTAGCTGGAGGTGGTCGACCTTTGTCTGGATTGGTTTCTGCAGAACATGCCAAGTTAATGGCATGTATAATGGCAATTGATTTCCTACTGCAGCAGGGCTTTATGCCGGTCATGATACTGGAAACTGATGCGCTGATGGTGCAACGCCAATTGACTTCTCAAGCGGGGATTAACACATCGATTCGTAGTCGATTGTATAAAGATTTGGCTGTGGTTTTGGCTGCAAACACAGACATTTCAGTCACCTATAAGGGTAGGAATGCTAATATGGCAGCTCATATCCTTGCTGCCCATGCGTGCAATGAACCTCAAGAGTTCTTCTTTTTTTCTACACCTTCTTTTCTTCAAGCTGCTATTGCAGCTGATGCTTGCTTGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko04657 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04657 map04914 map05166
Pfam Domains
Protein Families
ANAPC5

Protein Analysis

127

Amino Acids

13.64

Weight (kDa)

6.24

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 4 - 100 4.4e-11 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
AccI GTMKAC 1 cut(s) 19
AciI CCGC 1 cut(s) 167
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 350
AluBI AGCT 4 cut(s) 8, 278, 353, 365
AluI AGCT 4 cut(s) 8, 278, 353, 365
ApeKI GCWGC 6 cut(s) 94, 224, 275, 290, 353, 362
AspLEI GCGC 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 238
BbvI GCAGC 6 cut(s) 106, 211, 277, 287, 340, 374
BccI CCATC 1 cut(s) 133
BfmI CTRYAG 2 cut(s) 42, 92
BisI GCNGC 6 cut(s) 95, 225, 276, 291, 354, 363
BlsI GCNGC 6 cut(s) 96, 226, 277, 292, 355, 364
BmsI GCATC 2 cut(s) 121, 358
BoxI GACNNNNGTC 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 30
BpuEI CTTGAG 2 cut(s) 147, 297
Bsa29I ATCGAT 1 cut(s) 182
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 109
Bse1I ACTGG 1 cut(s) 126
Bse3DI GCAATG 1 cut(s) 310
BseCI ATCGAT 1 cut(s) 182
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMI GCAATG 1 cut(s) 310
BseNI ACTGG 1 cut(s) 126
BseXI GCAGC 6 cut(s) 106, 211, 277, 287, 340, 374
BshVI ATCGAT 1 cut(s) 182
BsiSI CCGG 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 29
BsmI GAATGC 1 cut(s) 268
BspACI CCGC 1 cut(s) 167
BspDI ATCGAT 1 cut(s) 182
BspHI TCATGA 1 cut(s) 114
BspMAI CTGCAG 2 cut(s) 46, 96
BsrDI GCAATG 1 cut(s) 310
BsrFI RCCGGY 1 cut(s) 109
BsrI ACTGG 1 cut(s) 126
BssAI RCCGGY 1 cut(s) 109
BstAPI GCANNNNNTGC 2 cut(s) 50, 368
BstC8I GCNNGC 2 cut(s) 301, 373
BstHHI GCGC 1 cut(s) 136
BstMWI GCNNNNNNNGC 7 cut(s) 50, 142, 272, 296, 359, 362, 368
BstNSI RCATGY 2 cut(s) 53, 69
BstPAI GACNNNNGTC 1 cut(s) 25
BstSFI CTRYAG 2 cut(s) 42, 92
BstV1I GCAGC 6 cut(s) 106, 211, 277, 287, 340, 374
BstXI CCANNNNNNTGG 1 cut(s) 61
Bsu15I ATCGAT 1 cut(s) 182
BsuTUI ATCGAT 1 cut(s) 182
Cac8I GCNNGC 2 cut(s) 301, 373
CciI TCATGA 1 cut(s) 114
CfoI GCGC 1 cut(s) 136
Cfr10I RCCGGY 1 cut(s) 109
ClaI ATCGAT 1 cut(s) 182
CviAII CATG 4 cut(s) 50, 66, 115, 296
CviJI RGCY 7 cut(s) 8, 102, 212, 224, 278, 353, 365
CviKI_1 RGCY 7 cut(s) 8, 102, 212, 224, 278, 353, 365
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
FaeI CATG 4 cut(s) 53, 69, 118, 299
FatI CATG 4 cut(s) 49, 65, 114, 295
FauI CCCGC 1 cut(s) 160
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 6 cut(s) 95, 225, 276, 291, 354, 363
Fsp4HI GCNGC 6 cut(s) 95, 225, 276, 291, 354, 363
GlaI GCGC 1 cut(s) 135
GluI GCNGC 6 cut(s) 95, 225, 276, 291, 354, 363
GsuI CTGGAG 1 cut(s) 30
HapII CCGG 1 cut(s) 110
HhaI GCGC 1 cut(s) 136
Hin1II CATG 4 cut(s) 53, 69, 118, 299
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HincII GTYRAC 1 cut(s) 20
HindII GTYRAC 1 cut(s) 20
HinfI GANTC 1 cut(s) 184
HpaII CCGG 1 cut(s) 110
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 1 cut(s) 20
Hpy188III TCNNGA 3 cut(s) 30, 115, 314
Hpy8I GTNNAC 1 cut(s) 20
HpyAV CCTTC 1 cut(s) 348
HpyCH4V TGCA 6 cut(s) 44, 94, 145, 227, 303, 362
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 142, 272, 296, 359, 362, 368
Hsp92II CATG 4 cut(s) 53, 69, 118, 299
HspAI GCGC 1 cut(s) 134
LpnPI CCDG 4 cut(s) 15, 83, 107, 123
Lsp1109I GCAGC 6 cut(s) 106, 211, 277, 287, 340, 374
LweI GCATC 2 cut(s) 121, 358
MaeIII GTNAC 1 cut(s) 244
MboII GAAGA 2 cut(s) 313, 338
MfeI CAATTG 2 cut(s) 78, 152
MluCI AATT 2 cut(s) 78, 152
MnlI CCTC 2 cut(s) 5, 321
MseI TTAA 2 cut(s) 59, 174
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 110
MunI CAATTG 2 cut(s) 78, 152
Mva1269I GAATGC 1 cut(s) 268
MwoI GCNNNNNNNGC 7 cut(s) 50, 142, 272, 296, 359, 362, 368
NlaIII CATG 4 cut(s) 53, 69, 118, 299
NmuCI GTSAC 1 cut(s) 244
NspI RCATGY 2 cut(s) 53, 69
PagI TCATGA 1 cut(s) 114
PctI GAATGC 1 cut(s) 268
PfeI GAWTC 1 cut(s) 184
PkrI GCNGC 6 cut(s) 96, 226, 277, 292, 355, 364
PshAI GACNNNNGTC 1 cut(s) 25
PstI CTGCAG 2 cut(s) 46, 96
PvuII CAGCTG 1 cut(s) 365
SalI GTCGAC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 59, 174
SatI GCNGC 6 cut(s) 95, 225, 276, 291, 354, 363
SetI ASST 9 cut(s) 10, 16, 25, 251, 280, 313, 340, 355, 367
SfaNI GCATC 2 cut(s) 121, 358
SfcI CTRYAG 2 cut(s) 42, 92
SmlI CTYRAG 2 cut(s) 162, 312
SmoI CTYRAG 2 cut(s) 162, 312
Sse9I AATT 2 cut(s) 78, 152
SsiI CCGC 1 cut(s) 167
TaqI TCGA 3 cut(s) 19, 182, 193
TasI AATT 2 cut(s) 78, 152
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 2 cut(s) 59, 174
Tru9I TTAA 2 cut(s) 59, 174
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 6 cut(s) 94, 224, 275, 290, 353, 362
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 1 cut(s) 321
XceI RCATGY 2 cut(s) 53, 69
XmiI GTMKAC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.