MD09G1188100.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015666
Physical Location & Seq
Forward (+)
16432508 .. 16436779
4272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1188100.v1.1.491

Sequence Viewer

Length: 2283 bp
ATGGCACTTTTCAGACAATCATCAGCGGCCGGGGACCCCGGCTCCACCGTGGTCGCCATTGACAGAGACAAGAACAGCCAATTGGCAGTCAAATGGGCAGTTGATAATCTTTTGAGCAATAGGACCTCTCATTGCATCCTCATTCACGTTCGAAACCGCAGCTTGCATCCTCAGGATGTTGGTTTAGTTTCCAAAGATGGTCGCCCGCCAACTGAAGACGAGCTGCAACAGTTCTTTCTTCCCTACCGAGGATTCTGCGCACGAAAAGGGATTGTAGCCAAGGAAGTGATCATCCACGACATCGATGTTCCAAGTGCGCTCATTGATTATATTGTCAACAACTCCATATGCAATATTGTTGTCGGCGCTTCCAGTCGCAATGCTATAACAAGAAAATTTAAGGATTCAGATGTGCCTACTTGCTTATTCAAATCTGTACCAGAAACCTGTGCGGTATATGTCATATCGAAAGGAAGAATTCAGACTTCAGGGTCGGCAAGCAGACCTCAAACACCGGGGAGTAATGCAGTTACACCGAGAAGTAGGGCAGTTACACCAAGAAGTAGCGCAGTTACTCCCAGGAAAAGTTCTCTACAGGCACAGCTTTTCAACCATACTCATGGCGTGTCGGACTTCGAAGATGTCATCAGAAAACATTTTAGTGACGGGAGCTCAAACAGTGCTAGCTCTGACAGAGTATCTTTAGATAAGAGCACCGAGTTTAAGCAGTTAATGCCACCAACGGCGTATAGGCCTGGCAGCACAACAAATTCACCAACACAGTCAGTTGCAAGCTTCACGTCAGAAACTTCATCTCATCGGAATTCTGCATCAGGTTGCAGTGATCTCTCAGGGCCATTTAGTTTCCAATCAAATGGTTCAGATAATGAAGGTTCTGGTAACTCTTCCAACTCGCAAACCCCATCTGGCTTGGATGCCGAAATGAGGAGATTAAGGGTCGAACTGAAGCAAACCATGGACATGTATACTTCAGCTTGCAAAGAAGCTACTACAGCTAAACAGAATGCGTGGGAGATGCAAAAATGGAGGACGTCAGAAGAGCGCAAGTTGGAGGAAGCCAAGCTAGCGGAAGAGGCTGCGCTGGCAATGGCAGATATGGAAAGGCAGAAGAGCAAAGCTGCAACGGAAGCAGCACGGATGGCACAAAGGCTAGCAGATCTGGAAACACAGAAGAGAAAAATTGCAGAAAGGAAAGCCAAGCAGGAAGCTGAAGAGAGCAGGAGAGCTATAGATGCTTTGGTGAACAATCAAGTTCGGTACAGAAGATACACCATTGAAGAGATTGAAATTGCAACAGATTACTTTAACAATTCAAACAAGATTGGAGAAGGTGGATATGGACCAGTTTATAGAGCGATTCTTGATCACACTCCTGTTGCCATCAAGGTCTTGAGGCCGGACATATCACAGGGGCAGAGGCAATTCCAACAGGAGGTTGAGGTTTTGAGCTGTATTAGGCATCCACACATGGTTATCCTAGTAGGCGCCTGCCCCGAGTATGGATGCCTTGTTTATGAGTACATGGAAAATGGAAGCTTAGAAGACCGCCTCTTCTGCAAGAACAATACTCCTCCAATCTCATGGGCAATTCGTTTCAAAATATCTGCTGAAATTGCCACTGCCCTCCTTTTCCTTCACCAAACAAAACCCGAGCCTCTGGTGCATCGTGACCTCAAGCCAGGAAACATTCTCGTAAACCGAAACTACGTAAGTAAGATTGCGGATGTAGGCTTGGCTAGGCTAGTTCCACCATCTATAGCTGATAGCGTCACTCAATACCGGATGACAGCAGCAGCTGGTACATTTTGTTACATTGATCCGGAGTATCAACAAACAGGAATGTTGGGTGTGAAATCGGACATATATTCTTTAGGAGTAATGCTATTGCAGATCATTACCGCAAGGCCTGCAATGGGTTTATCCCACCAAGTTGAGAAGGCTATCGAGAAAGGTACATTTGCTGAGATGCTTGATCCTGCAGTGACAAATTGGCCAGTTGAAGAGGCTTTATCATTTGCAAAACTAGCTTTGAAGTGTTGTGAGCTGAGGAAGAAAGATAGACCAGATCTCAGTACAGTCATCTTGCCTGAGCTGAACCGGCTGAGAGATCTCGGGATGAAGAATGAAGCGACTGAAGTTTCTTGTGGACATTATTCATACACGGGGTCACTTCCACCGATGGCGTCAACATCAACTCAGAGTCAGGATGGAATGAGAGAAAATGCTAATGTCGAAATGGAAATTCAACGTAGATCAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0009507 GO:0009536 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Usp

Protein Analysis

761

Amino Acids

84.08

Weight (kDa)

8.36

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 17 - 131 4.5e-08 Universal stress protein family
Pkinase PF00069 443 - 698 1.4e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 445 - 700 1.1e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015666)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g34160 FvH4_6g34160
malus_domestica MD09G1188100.v1.1
prunus_persica Prupe.3G045000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0144481
rosa_laevigata RLG00000020084
rosa_multiflora Rmu_sc0005789.1_g000009 Rmu_sc0005789.1_g000011 Rmu_sc0007240.1_g000006
rosa_roxburghii Rroxscaffold_2G00101260
rosa_rugosa Rorug02G0386900
rosa_samantha Rh2AG439300 Rh2BG449500 Rh2CG426300 Rh2DG459300
rosa_wichuraiana Rw2G035940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 490
AatII GACGTC 1 cut(s) 1055
Acc16I TGCGCA 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 1505
AccI GTMKAC 1 cut(s) 986
AccIII TCCGGA 1 cut(s) 1840
AciI CCGC 8 cut(s) 26, 157, 206, 452, 1088, 1567, 1742, 1920
AclWI GGATC 2 cut(s) 1832, 1988
AcoI YGGCCR 2 cut(s) 27, 2012
AcsI RAATTY 5 cut(s) 395, 477, 769, 823, 2262
AcuI CTGAAG 6 cut(s) 234, 471, 975, 986, 1251, 2175
AcyI GRCGYC 3 cut(s) 1052, 1506, 2204
AfaI GTAC 6 cut(s) 438, 1280, 1541, 1822, 1975, 2095
AfiI CCNNNNNNNGG 5 cut(s) 248, 945, 1453, 1520, 1934
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 9 cut(s) 430, 610, 1298, 1307, 1335, 1618, 2021, 2053, 2267
AjiI CACGTC 1 cut(s) 801
AjnI CCWGG 3 cut(s) 578, 754, 1699
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
Alw21I GWGCWC 2 cut(s) 674, 716
Alw26I GTCTC 1 cut(s) 60
AlwI GGATC 2 cut(s) 1832, 1988
AlwNI CAGNNNCTG 1 cut(s) 1817
Ama87I CYCGRG 3 cut(s) 1514, 1670, 2132
Aor13HI TCCGGA 1 cut(s) 1840
AoxI GGCC 6 cut(s) 27, 752, 854, 1415, 1925, 2012
ApeKI GCWGC 8 cut(s) 159, 223, 759, 1097, 1139, 1151, 1811, 1814
ApoI RAATTY 5 cut(s) 395, 477, 769, 823, 2262
AspLEI GCGC 7 cut(s) 260, 319, 368, 569, 1065, 1102, 1508
AspS9I GGNCC 4 cut(s) 34, 123, 854, 1361
AsuC2I CCSGG 3 cut(s) 31, 39, 516
AsuHPI GGTGA 3 cut(s) 765, 1273, 1649
AsuII TTCGAA 2 cut(s) 151, 636
AsuNHI GCTAGC 3 cut(s) 683, 1084, 1171
AvaI CYCGRG 3 cut(s) 1514, 1670, 2132
AvaII GGWCC 3 cut(s) 34, 123, 1361
AxyI CCTNAGG 1 cut(s) 171
BalI TGGCCA 1 cut(s) 2014
BanI GGYRCC 1 cut(s) 1505
BanII GRGCYC 1 cut(s) 674
BbsI GAAGAC 2 cut(s) 222, 1569
Bbv12I GWGCWC 2 cut(s) 674, 716
BbvCI CCTCAGC 1 cut(s) 2066
BbvI GCAGC 8 cut(s) 171, 210, 771, 1084, 1126, 1163, 1823, 1826
BccI CCATC 7 cut(s) 191, 931, 1153, 1409, 1780, 2194, 2222
BceAI ACGGC 1 cut(s) 759
BcgI CGANNNNNNTGC 2 cut(s) 237, 271
BciT130I CCWGG 3 cut(s) 580, 756, 1701
BclI TGATCA 2 cut(s) 288, 1384
BcnI CCSGG 3 cut(s) 31, 39, 516
BcoDI GTCTC 1 cut(s) 60
BfaI CTAG 7 cut(s) 684, 1085, 1172, 1499, 1758, 1763, 2045
BfmI CTRYAG 5 cut(s) 593, 1011, 1248, 1776, 1998
BfoI RGCGCY 2 cut(s) 369, 1509
BglII AGATCT 3 cut(s) 1177, 2086, 2128
BisI GCNGC 9 cut(s) 27, 160, 224, 760, 1098, 1140, 1152, 1812, 1815
BlsI GCNGC 9 cut(s) 28, 161, 225, 761, 1099, 1141, 1153, 1813, 1816
Bme1390I CCNGG 6 cut(s) 31, 39, 516, 580, 756, 1701
Bme18I GGWCC 3 cut(s) 34, 123, 1361
BmeT110I CYCGRG 3 cut(s) 1514, 1670, 2132
BmgBI CACGTC 1 cut(s) 801
BmgT120I GGNCC 4 cut(s) 34, 123, 854, 1361
BmiI GGNNCC 4 cut(s) 35, 36, 43, 1507
BmrFI CCNGG 6 cut(s) 31, 39, 516, 580, 756, 1701
BmtI GCTAGC 3 cut(s) 687, 1088, 1175
BpiI GAAGAC 2 cut(s) 222, 1569
Bpu10I CCTNAGC 2 cut(s) 2066, 2109
Bpu14I TTCGAA 2 cut(s) 151, 636
BpuEI CTTGAG 2 cut(s) 1432, 1679
BpuMI CCSGG 3 cut(s) 31, 39, 516
Bsa29I ATCGAT 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 1729
BsaHI GRCGYC 3 cut(s) 1052, 1506, 2204
BsaJI CCNNGG 8 cut(s) 30, 37, 48, 247, 279, 515, 578, 975
BsaWI WCCGGW 2 cut(s) 1800, 1840
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 5 cut(s) 248, 945, 1453, 1520, 1934
Bse118I RCCGGY 1 cut(s) 2118
Bse1I ACTGG 3 cut(s) 372, 1364, 2015
Bse21I CCTNAGG 1 cut(s) 171
Bse3DI GCAATG 4 cut(s) 130, 385, 1113, 1938
BseAI TCCGGA 1 cut(s) 1840
BseBI CCWGG 3 cut(s) 580, 756, 1701
BseCI ATCGAT 1 cut(s) 303
BseDI CCNNGG 8 cut(s) 30, 37, 48, 247, 279, 515, 578, 975
BseLI CCNNNNNNNGG 5 cut(s) 248, 945, 1453, 1520, 1934
BseMI GCAATG 4 cut(s) 130, 385, 1113, 1938
BseMII CTCAG 8 cut(s) 185, 864, 1974, 2057, 2100, 2104, 2114, 2231
BseNI ACTGG 3 cut(s) 372, 1364, 2015
BseRI GAGGAG 2 cut(s) 961, 1581
BseX3I CGGCCG 1 cut(s) 27
BseXI GCAGC 8 cut(s) 171, 210, 771, 1084, 1126, 1163, 1823, 1826
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 6 cut(s) 29, 754, 856, 1417, 1927, 2014
BshNI GGYRCC 1 cut(s) 1505
BshVI ATCGAT 1 cut(s) 303
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 2 cut(s) 674, 716
BsiHKCI CYCGRG 3 cut(s) 1514, 1670, 2132
BsiSI CCGG 7 cut(s) 30, 39, 515, 1418, 1801, 1841, 2119
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 5 cut(s) 248, 945, 1453, 1520, 1934
BsmAI GTCTC 1 cut(s) 60
BsmFI GGGAC 1 cut(s) 47
BsmI GAATGC 1 cut(s) 1030
BsnI GGCC 6 cut(s) 29, 754, 856, 1417, 1927, 2014
BsoBI CYCGRG 3 cut(s) 1514, 1670, 2132
Bsp119I TTCGAA 2 cut(s) 151, 636
Bsp1286I GDGCHC 2 cut(s) 674, 716
Bsp13I TCCGGA 1 cut(s) 1840
Bsp19I CCATGG 1 cut(s) 975
BspACI CCGC 8 cut(s) 26, 157, 206, 452, 1088, 1567, 1742, 1920
BspANI GGCC 6 cut(s) 29, 754, 856, 1417, 1927, 2014
BspCNI CTCAG 8 cut(s) 184, 863, 1975, 2058, 2101, 2103, 2115, 2230
BspDI ATCGAT 1 cut(s) 303
BspEI TCCGGA 1 cut(s) 1840
BspLI GGNNCC 4 cut(s) 35, 36, 43, 1507
BspMAI CTGCAG 1 cut(s) 2002
BspOI GCTAGC 3 cut(s) 687, 1088, 1175
BspPI GGATC 2 cut(s) 1832, 1988
BspQI GCTCTTC 2 cut(s) 1053, 1124
BspT104I TTCGAA 2 cut(s) 151, 636
BspT107I GGYRCC 1 cut(s) 1505
BsrDI GCAATG 4 cut(s) 130, 385, 1113, 1938
BsrFI RCCGGY 1 cut(s) 2118
BsrI ACTGG 3 cut(s) 372, 1364, 2015
BssAI RCCGGY 1 cut(s) 2118
BssECI CCNNGG 8 cut(s) 30, 37, 48, 247, 279, 515, 578, 975
BssNAI GTATAC 1 cut(s) 987
BssNI GRCGYC 3 cut(s) 1052, 1506, 2204
BssT1I CCWWGG 2 cut(s) 279, 975
Bst1107I GTATAC 1 cut(s) 987
Bst2UI CCWGG 3 cut(s) 580, 756, 1701
Bst4CI ACNGT 5 cut(s) 49, 231, 680, 783, 2098
Bst6I CTCTTC 9 cut(s) 910, 1053, 1086, 1124, 1187, 1227, 1293, 1577, 2016
BstACI GRCGYC 3 cut(s) 1052, 1506, 2204
BstAPI GCANNNNNTGC 2 cut(s) 733, 1928
BstBAI YACGTR 1 cut(s) 1729
BstBI TTCGAA 2 cut(s) 151, 636
BstDEI CTNAG 9 cut(s) 171, 850, 1558, 1983, 2066, 2090, 2109, 2123, 2217
BstDSI CCRYGG 2 cut(s) 48, 975
BstH2I RGCGCY 2 cut(s) 369, 1509
BstHHI GCGC 7 cut(s) 260, 319, 368, 569, 1065, 1102, 1508
BstMAI GTCTC 1 cut(s) 60
BstMCI CGRYCG 1 cut(s) 30
BstNI CCWGG 3 cut(s) 580, 756, 1701
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 6 cut(s) 29, 37, 514, 578, 754, 1699
BstSFI CTRYAG 5 cut(s) 593, 1011, 1248, 1776, 1998
BstSNI TACGTA 1 cut(s) 1729
BstV1I GCAGC 8 cut(s) 171, 210, 771, 1084, 1126, 1163, 1823, 1826
BstV2I GAAGAC 2 cut(s) 222, 1569
BstX2I RGATCY 3 cut(s) 1177, 2086, 2128
BstXI CCANNNNNNTGG 3 cut(s) 620, 875, 1602
BstYI RGATCY 3 cut(s) 1177, 2086, 2128
BstZ17I GTATAC 1 cut(s) 987
BstZI CGGCCG 1 cut(s) 27
Bsu15I ATCGAT 1 cut(s) 303
Bsu36I CCTNAGG 1 cut(s) 171
BsuRI GGCC 6 cut(s) 29, 754, 856, 1417, 1927, 2014
BsuTUI ATCGAT 1 cut(s) 303
BtgI CCRYGG 2 cut(s) 48, 975
BtrI CACGTC 1 cut(s) 801
BtsI GCAGTG 3 cut(s) 847, 1638, 2007
BtsIMutI CAGTG 4 cut(s) 685, 847, 1638, 2007
CaiI CAGNNNCTG 1 cut(s) 1817
CfoI GCGC 7 cut(s) 260, 319, 368, 569, 1065, 1102, 1508
Cfr10I RCCGGY 1 cut(s) 2118
Cfr13I GGNCC 4 cut(s) 34, 123, 854, 1361
ClaI ATCGAT 1 cut(s) 303
CseI GACGC 2 cut(s) 1777, 2193
Csp6I GTAC 6 cut(s) 437, 1279, 1540, 1821, 1974, 2094
CviAII CATG 6 cut(s) 620, 976, 982, 1489, 1543, 1602
CviQI GTAC 6 cut(s) 437, 1279, 1540, 1821, 1974, 2094
DdeI CTNAG 9 cut(s) 171, 850, 1558, 1983, 2066, 2090, 2109, 2123, 2217
DinI GGCGCC 1 cut(s) 1507
DrdI GACNNNNNNGTC 1 cut(s) 490
DseDI GACNNNNNNGTC 1 cut(s) 490
EaeI YGGCCR 2 cut(s) 27, 2012
EagI CGGCCG 1 cut(s) 27
Eam1104I CTCTTC 9 cut(s) 910, 1053, 1086, 1124, 1187, 1227, 1293, 1577, 2016
EarI CTCTTC 9 cut(s) 910, 1053, 1086, 1124, 1187, 1227, 1293, 1577, 2016
Ecl136II GAGCTC 1 cut(s) 672
EclXI CGGCCG 1 cut(s) 27
Eco105I TACGTA 1 cut(s) 1729
Eco130I CCWWGG 2 cut(s) 279, 975
Eco147I AGGCCT 2 cut(s) 754, 1927
Eco24I GRGCYC 1 cut(s) 674
Eco47I GGWCC 3 cut(s) 34, 123, 1361
Eco52I CGGCCG 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 672
Eco57I CTGAAG 6 cut(s) 234, 471, 975, 986, 1251, 2175
Eco81I CCTNAGG 1 cut(s) 171
Eco88I CYCGRG 3 cut(s) 1514, 1670, 2132
EcoICRI GAGCTC 1 cut(s) 672
EcoO109I RGGNCCY 2 cut(s) 34, 123
EcoRI GAATTC 2 cut(s) 477, 823
EcoRII CCWGG 3 cut(s) 578, 754, 1699
EcoT14I CCWWGG 2 cut(s) 279, 975
EcoT38I GRGCYC 1 cut(s) 674
EgeI GGCGCC 1 cut(s) 1507
EheI GGCGCC 1 cut(s) 1507
ErhI CCWWGG 2 cut(s) 279, 975
FaeI CATG 6 cut(s) 623, 979, 985, 1492, 1546, 1605
FaqI GGGAC 1 cut(s) 47
FatI CATG 6 cut(s) 619, 975, 981, 1488, 1542, 1601
FauI CCCGC 1 cut(s) 213
FauNDI CATATG 1 cut(s) 347
FbaI TGATCA 2 cut(s) 288, 1384
FblI GTMKAC 1 cut(s) 986
Fnu4HI GCNGC 9 cut(s) 27, 160, 224, 760, 1098, 1140, 1152, 1812, 1815
FriOI GRGCYC 1 cut(s) 674
Fsp4HI GCNGC 9 cut(s) 27, 160, 224, 760, 1098, 1140, 1152, 1812, 1815
FspBI CTAG 7 cut(s) 684, 1085, 1172, 1499, 1758, 1763, 2045
FspI TGCGCA 1 cut(s) 259
GlaI GCGC 7 cut(s) 259, 318, 367, 568, 1064, 1101, 1507
GluI GCNGC 9 cut(s) 27, 160, 224, 760, 1098, 1140, 1152, 1812, 1815
HaeII RGCGCY 2 cut(s) 369, 1509
HaeIII GGCC 6 cut(s) 29, 754, 856, 1417, 1927, 2014
HapII CCGG 7 cut(s) 30, 39, 515, 1418, 1801, 1841, 2119
HgaI GACGC 2 cut(s) 1777, 2193
HhaI GCGC 7 cut(s) 260, 319, 368, 569, 1065, 1102, 1508
Hin1I GRCGYC 3 cut(s) 1052, 1506, 2204
Hin1II CATG 6 cut(s) 623, 979, 985, 1492, 1546, 1605
Hin6I GCGC 7 cut(s) 258, 317, 366, 567, 1063, 1100, 1506
HinP1I GCGC 7 cut(s) 258, 317, 366, 567, 1063, 1100, 1506
HincII GTYRAC 2 cut(s) 337, 2208
HindII GTYRAC 2 cut(s) 337, 2208
HindIII AAGCTT 2 cut(s) 793, 1555
HinfI GANTC 4 cut(s) 252, 404, 1378, 2221
HpaII CCGG 7 cut(s) 30, 39, 515, 1418, 1801, 1841, 2119
HphI GGTGA 3 cut(s) 765, 1273, 1649
Hpy166II GTNNAC 6 cut(s) 337, 987, 1264, 1717, 2168, 2208
Hpy188III TCNNGA 9 cut(s) 173, 1181, 1382, 1411, 1688, 1841, 1966, 2134, 2225
Hpy8I GTNNAC 6 cut(s) 337, 987, 1264, 1717, 2168, 2208
HpyAV CCTTC 4 cut(s) 884, 1343, 1664, 1951
HpyCH4III ACNGT 5 cut(s) 49, 231, 680, 783, 2098
HpyCH4IV ACGT 5 cut(s) 147, 800, 1052, 1728, 2269
HpyF3I CTNAG 9 cut(s) 171, 850, 1558, 1983, 2066, 2090, 2109, 2123, 2217
HpySE526I ACGT 5 cut(s) 147, 800, 1052, 1728, 2269
Hsp92I GRCGYC 3 cut(s) 1052, 1506, 2204
Hsp92II CATG 6 cut(s) 623, 979, 985, 1492, 1546, 1605
HspAI GCGC 7 cut(s) 258, 317, 366, 567, 1063, 1100, 1506
KasI GGCGCC 1 cut(s) 1505
KflI GGGWCCC 1 cut(s) 34
Kpn2I TCCGGA 1 cut(s) 1840
Ksp22I TGATCA 2 cut(s) 288, 1384
LguI GCTCTTC 2 cut(s) 1053, 1124
LmnI GCTCC 2 cut(s) 47, 669
Lsp1109I GCAGC 8 cut(s) 171, 210, 771, 1084, 1126, 1163, 1823, 1826
MaeI CTAG 7 cut(s) 684, 1085, 1172, 1499, 1758, 1763, 2045
MaeII ACGT 5 cut(s) 147, 800, 1052, 1728, 2269
MfeI CAATTG 1 cut(s) 80
MflI RGATCY 3 cut(s) 1177, 2086, 2128
MhlI GDGCHC 2 cut(s) 674, 716
MlsI TGGCCA 1 cut(s) 2014
MluNI TGGCCA 1 cut(s) 2014
Mly113I GGCGCC 1 cut(s) 1506
MlyI GAGTC 1 cut(s) 2230
MmeI TCCRAC 4 cut(s) 609, 933, 1050, 1471
Mox20I TGGCCA 1 cut(s) 2014
MroI TCCGGA 1 cut(s) 1840
MscI TGGCCA 1 cut(s) 2014
MseI TTAA 5 cut(s) 399, 723, 731, 953, 1326
MslI CAYNNNNRTG 3 cut(s) 618, 660, 980
Msp20I TGGCCA 1 cut(s) 2014
MspA1I CMGCKG 2 cut(s) 26, 1817
MspI CCGG 7 cut(s) 30, 39, 515, 1418, 1801, 1841, 2119
MspR9I CCNGG 6 cut(s) 31, 39, 516, 580, 756, 1701
MunI CAATTG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 1030
MvaI CCWGG 3 cut(s) 580, 756, 1701
NarI GGCGCC 1 cut(s) 1506
NciI CCSGG 3 cut(s) 31, 39, 516
NcoI CCATGG 1 cut(s) 975
NdeI CATATG 1 cut(s) 347
NheI GCTAGC 3 cut(s) 683, 1084, 1171
NlaIII CATG 6 cut(s) 623, 979, 985, 1492, 1546, 1605
NlaIV GGNNCC 4 cut(s) 35, 36, 43, 1507
NmuCI GTSAC 5 cut(s) 662, 1688, 1789, 2002, 2187
NsbI TGCGCA 1 cut(s) 259
NspI RCATGY 1 cut(s) 985
NspV TTCGAA 2 cut(s) 151, 636
PceI AGGCCT 2 cut(s) 754, 1927
PciI ACATGT 1 cut(s) 981
PciSI GCTCTTC 2 cut(s) 1053, 1124
PctI GAATGC 1 cut(s) 1030
PfeI GAWTC 3 cut(s) 252, 404, 1378
PkrI GCNGC 9 cut(s) 28, 161, 225, 761, 1099, 1141, 1153, 1813, 1816
PleI GAGTC 1 cut(s) 2229
PluTI GGCGCC 1 cut(s) 1509
PpsI GAGTC 1 cut(s) 2229
Ppu21I YACGTR 1 cut(s) 1729
PpuMI RGGWCCY 2 cut(s) 34, 123
PscI ACATGT 1 cut(s) 981
Psp124BI GAGCTC 1 cut(s) 674
Psp5II RGGWCCY 2 cut(s) 34, 123
Psp6I CCWGG 3 cut(s) 578, 754, 1699
PspGI CCWGG 3 cut(s) 578, 754, 1699
PspN4I GGNNCC 4 cut(s) 35, 36, 43, 1507
PspPI GGNCC 4 cut(s) 34, 123, 854, 1361
PspPPI RGGWCCY 2 cut(s) 34, 123
PstI CTGCAG 1 cut(s) 2002
PstNI CAGNNNCTG 1 cut(s) 1817
PsuI RGATCY 3 cut(s) 1177, 2086, 2128
PvuII CAGCTG 1 cut(s) 1817
RsaI GTAC 6 cut(s) 438, 1280, 1541, 1822, 1975, 2095
RsaNI GTAC 6 cut(s) 437, 1279, 1540, 1821, 1974, 2094
RseI CAYNNNNRTG 3 cut(s) 618, 660, 980
SacI GAGCTC 1 cut(s) 674
SapI GCTCTTC 2 cut(s) 1053, 1124
SaqAI TTAA 5 cut(s) 399, 723, 731, 953, 1326
SatI GCNGC 9 cut(s) 27, 160, 224, 760, 1098, 1140, 1152, 1812, 1815
Sau96I GGNCC 4 cut(s) 34, 123, 854, 1361
SchI GAGTC 1 cut(s) 2230
ScrFI CCNGG 6 cut(s) 31, 39, 516, 580, 756, 1701
SduI GDGCHC 2 cut(s) 674, 716
SfcI CTRYAG 5 cut(s) 593, 1011, 1248, 1776, 1998
SfoI GGCGCC 1 cut(s) 1507
SfuI TTCGAA 2 cut(s) 151, 636
SinI GGWCC 3 cut(s) 34, 123, 1361
SmiMI CAYNNNNRTG 3 cut(s) 618, 660, 980
SmlI CTYRAG 2 cut(s) 1411, 1694
SmoI CTYRAG 2 cut(s) 1411, 1694
SnaBI TACGTA 1 cut(s) 1729
SseBI AGGCCT 2 cut(s) 754, 1927
SsiI CCGC 8 cut(s) 26, 157, 206, 452, 1088, 1567, 1742, 1920
SspDI GGCGCC 1 cut(s) 1505
SspI AATATT 1 cut(s) 355
SspMI CTAG 7 cut(s) 684, 1085, 1172, 1499, 1758, 1763, 2045
SstI GAGCTC 1 cut(s) 674
StuI AGGCCT 2 cut(s) 754, 1927
StyD4I CCNGG 6 cut(s) 29, 37, 514, 578, 754, 1699
StyI CCWWGG 2 cut(s) 279, 975
TaaI ACNGT 5 cut(s) 49, 231, 680, 783, 2098
TaiI ACGT 5 cut(s) 150, 803, 1055, 1731, 2272
TaqI TCGA 7 cut(s) 151, 303, 467, 636, 960, 1965, 2253
TatI WGTACW 2 cut(s) 1539, 2093
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 3 cut(s) 252, 404, 1378
Tru1I TTAA 5 cut(s) 399, 723, 731, 953, 1326
Tru9I TTAA 5 cut(s) 399, 723, 731, 953, 1326
TscAI CASTG 4 cut(s) 685, 847, 1645, 2007
TseFI GTSAC 5 cut(s) 662, 1688, 1789, 2002, 2187
TseI GCWGC 8 cut(s) 159, 223, 759, 1097, 1139, 1151, 1811, 1814
Tsp45I GTSAC 5 cut(s) 662, 1688, 1789, 2002, 2187
TspDTI ATGAA 5 cut(s) 801, 903, 2153, 2160, 2166
TspGWI ACGGA 2 cut(s) 1160, 1171
TspRI CASTG 4 cut(s) 685, 847, 1645, 2007
VpaK11BI GGWCC 3 cut(s) 34, 123, 1361
XapI RAATTY 5 cut(s) 395, 477, 769, 823, 2262
XceI RCATGY 1 cut(s) 985
XmiI GTMKAC 1 cut(s) 986
XspI CTAG 7 cut(s) 684, 1085, 1172, 1499, 1758, 1763, 2045
ZraI GACGTC 1 cut(s) 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.