RchiOBHm_Chr2g0144481

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015666
Physical Location & Seq
Reverse (-)
62082195 .. 62083727
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51442

Sequence Viewer

Length: 1356 bp
ATGTTATATGTGTTACAGTCTGGCTTGGATGGTGAGATGATGAGATTAAGGCAGGAATTGAAGCAAACAATGGACATGTACACTTCAGCTTGCAAAGAAGCTTTAGCATCTAAGCAGAAGGCATGGGAGCTCCAAAAATGGAAAACAGCGAAAGAGCGCAAGTTAGAGGAAGCCAAGCTTGCTGAAGAGGCTGCTGTGGCCTTAGCAGAGAAGGAGAAGCAGAAAAGCAAGGCTGCAATAGATGCAGCGCGGATGGCACATAGGCTAGCAGAGATGGAGACCCAGAAGAGGAAAATTGCAGAAATGAGAGCCAAGGAGGAGGCTAAAGAGAGAAGGAAAGCAATGGAGGCTCTGGCACAGAACAAAGCTATGTATAGGAGATACTCCATTGAAGAGATTGAAGTTGCCACAGATTACTTTAACATTTCAAACAAGGTTGGGGAAGGTGGATATGGACCTGTTTATAAAGGCCTGCTTAACCACACCCCAGTTGCCATCAAGGTCTTGAGGCCAGACATTTCACAGGGGAAGAGGCAATTCCAACAAGAGGTTGAGGTTCTAAGCTGCATTAGACATCCACACATGGTTCTCTTAGTAGGTACCTGCCCTGAGTATGGATGCCTTGTATATGAGTACATGGAAAACGGAAGCTTAGAAGACCGGCTCTTCCAGAAGGACAACACGCCTCCAATCCCATGGACTGTCCGTTTCAAAATAGCTGCAGAAATTGCCACTGGCCTTCTTTTTCTTCACCAGACAAAACCAGAACCCATTGTGCACCGTGATCTCAAGCCGGCAAACATTCTCCTAGACCGGAACTGTGTGAGCAAGATTGCTGATGTAGGCTTATCCAGGCTAGTCCCGGCATCTGTGGCTGATAGTGTCACTCAATACCGCCTGACAGCAGCAGCCGGTACATTTTGCTACATTGACCCGGAGTATCAGAAAACAGGAATGTTGGGTGTGAAATCAGACATATATTCCTTGGGAATTATGCTGTTACAGATTCTTACATCAAAGCCTGCAATGGCATTGTCCCACCAGGTTGAGGAGTCCATTGAGAAAGGTACATTCGCGGAGTTGCTTGATCCTGCAGTGCCAGACTGGCCTGTTGAAGAGGCTTTGTCATTAGCAAAACTGGCTTTGAAGTGCTGTGAGCTCAGGAGGAGAGATAGACCGGATCTTGGTTCAGTCATCTTGCCTGAGCTGGACCGGCTAAGAGAGTTTGGGAATCAGAAGGAAGCCAATAACTATGCCATCGTTCCCTATCGAGGGCATTCACACGCATCACTTCCACCAGTACCAGTAGGGAGATCAACCACAAATGCCAGTCAGGTTAGTTTCTCTGCAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0009507 GO:0009536 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Usp

Protein Analysis

451

Amino Acids

50.68

Weight (kDa)

8.25

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 141 - 394 2.6e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 141 - 397 4.6e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015666)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g34160 FvH4_6g34160
malus_domestica MD09G1188100.v1.1
prunus_persica Prupe.3G045000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0144481
rosa_laevigata RLG00000020084
rosa_multiflora Rmu_sc0005789.1_g000009 Rmu_sc0005789.1_g000011 Rmu_sc0007240.1_g000006
rosa_roxburghii Rroxscaffold_2G00101260
rosa_rugosa Rorug02G0386900
rosa_samantha Rh2AG439300 Rh2BG449500 Rh2CG426300 Rh2DG459300
rosa_wichuraiana Rw2G035940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 465
Acc36I ACCTGC 1 cut(s) 611
Acc65I GGTACC 1 cut(s) 599
AccB1I GGYRCC 1 cut(s) 599
AccII CGCG 2 cut(s) 250, 1076
AciI CCGC 3 cut(s) 250, 895, 1076
AclWI GGATC 2 cut(s) 1082, 1188
AcuI CTGAAG 2 cut(s) 69, 204
AfaI GTAC 6 cut(s) 80, 601, 635, 916, 1069, 1302
AfiI CCNNNNNNNGG 7 cut(s) 288, 547, 614, 1048, 1184, 1271, 1272
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 7 cut(s) 61, 392, 401, 429, 712, 1115, 1147
AjnI CCWGG 2 cut(s) 851, 1041
Alw21I GWGCWC 3 cut(s) 132, 780, 1161
Alw26I GTCTC 1 cut(s) 272
Alw44I GTGCAC 1 cut(s) 776
AlwI GGATC 2 cut(s) 1082, 1188
AoxI GGCC 5 cut(s) 198, 469, 509, 736, 1106
ApaLI GTGCAC 1 cut(s) 776
ApeKI GCWGC 7 cut(s) 191, 233, 245, 564, 719, 905, 908
Asp718I GGTACC 1 cut(s) 599
AspLEI GCGC 2 cut(s) 159, 250
AspS9I GGNCC 2 cut(s) 455, 1210
AsuC2I CCSGG 2 cut(s) 863, 935
AsuHPI GGTGA 2 cut(s) 44, 743
AsuNHI GCTAGC 1 cut(s) 265
AvaII GGWCC 2 cut(s) 455, 1210
BaeGI GKGCMC 1 cut(s) 780
BanI GGYRCC 1 cut(s) 599
BanII GRGCYC 2 cut(s) 132, 1161
BbsI GAAGAC 1 cut(s) 663
Bbv12I GWGCWC 3 cut(s) 132, 780, 1161
BbvI GCAGC 7 cut(s) 178, 220, 257, 551, 706, 917, 920
BccI CCATC 5 cut(s) 23, 247, 268, 503, 1265
BciT130I CCWGG 2 cut(s) 853, 1043
BcnI CCSGG 2 cut(s) 863, 935
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 3 cut(s) 266, 809, 857
BfmI CTRYAG 3 cut(s) 720, 1092, 1347
BfuAI ACCTGC 1 cut(s) 611
BglI GCCNNNNNGGC 1 cut(s) 1105
BisI GCNGC 7 cut(s) 192, 234, 246, 565, 720, 906, 909
BlsI GCNGC 7 cut(s) 193, 235, 247, 566, 721, 907, 910
Bme1390I CCNGG 4 cut(s) 853, 863, 935, 1043
Bme18I GGWCC 2 cut(s) 455, 1210
BmgT120I GGNCC 2 cut(s) 455, 1210
BmiI GGNNCC 1 cut(s) 601
BmrFI CCNGG 4 cut(s) 853, 863, 935, 1043
BmrI ACTGGG 1 cut(s) 482
BmsI GCATC 5 cut(s) 116, 232, 608, 875, 1295
BmtI GCTAGC 1 cut(s) 269
BmuI ACTGGG 1 cut(s) 482
BpiI GAAGAC 1 cut(s) 663
Bpu10I CCTNAGC 3 cut(s) 202, 1160, 1203
BpuEI CTTGAG 2 cut(s) 526, 773
BpuMI CCSGG 2 cut(s) 863, 935
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 3 cut(s) 312, 695, 984
BsaWI WCCGGW 2 cut(s) 813, 1177
Bsc4I CCNNNNNNNGG 7 cut(s) 288, 547, 614, 1048, 1184, 1271, 1272
Bse118I RCCGGY 4 cut(s) 660, 793, 911, 1212
Bse1I ACTGG 7 cut(s) 488, 739, 1109, 1143, 1298, 1304, 1329
Bse3DI GCAATG 2 cut(s) 348, 1032
BseBI CCWGG 2 cut(s) 853, 1043
BseDI CCNNGG 3 cut(s) 312, 695, 984
BseGI GGATG 4 cut(s) 34, 258, 574, 623
BseLI CCNNNNNNNGG 7 cut(s) 288, 547, 614, 1048, 1184, 1271, 1272
BseMI GCAATG 2 cut(s) 348, 1032
BseMII CTCAG 3 cut(s) 600, 1174, 1194
BseNI ACTGG 7 cut(s) 488, 739, 1109, 1143, 1298, 1304, 1329
BseRI GAGGAG 3 cut(s) 332, 1064, 1180
BseSI GKGCMC 1 cut(s) 780
BseXI GCAGC 7 cut(s) 178, 220, 257, 551, 706, 917, 920
Bsh1236I CGCG 2 cut(s) 250, 1076
BshFI GGCC 5 cut(s) 200, 471, 511, 738, 1108
BshNI GGYRCC 1 cut(s) 599
BsiHKAI GWGCWC 3 cut(s) 132, 780, 1161
BsiSI CCGG 8 cut(s) 661, 794, 814, 863, 912, 935, 1178, 1213
BslFI GGGAC 2 cut(s) 845, 1021
BslI CCNNNNNNNGG 7 cut(s) 288, 547, 614, 1048, 1184, 1271, 1272
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 2 cut(s) 845, 1021
BsmI GAATGC 1 cut(s) 1276
BsnI GGCC 5 cut(s) 200, 471, 511, 738, 1108
Bso31I GGTCTC 1 cut(s) 272
Bsp1286I GDGCHC 3 cut(s) 132, 780, 1161
Bsp1407I TGTACA 1 cut(s) 78
Bsp143I GATC 4 cut(s) 784, 1087, 1180, 1313
Bsp19I CCATGG 1 cut(s) 695
BspACI CCGC 3 cut(s) 250, 895, 1076
BspANI GGCC 5 cut(s) 200, 471, 511, 738, 1108
BspCNI CTCAG 3 cut(s) 601, 1173, 1195
BspFNI CGCG 2 cut(s) 250, 1076
BspLI GGNNCC 1 cut(s) 601
BspMAI CTGCAG 3 cut(s) 724, 1096, 1351
BspMI ACCTGC 1 cut(s) 611
BspOI GCTAGC 1 cut(s) 269
BspPI GGATC 2 cut(s) 1082, 1188
BspQI GCTCTTC 1 cut(s) 671
BspT107I GGYRCC 1 cut(s) 599
BspTNI GGTCTC 1 cut(s) 272
BsrDI GCAATG 2 cut(s) 348, 1032
BsrFI RCCGGY 4 cut(s) 660, 793, 911, 1212
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 7 cut(s) 488, 739, 1109, 1143, 1298, 1304, 1329
BssAI RCCGGY 4 cut(s) 660, 793, 911, 1212
BssECI CCNNGG 3 cut(s) 312, 695, 984
BssMI GATC 4 cut(s) 784, 1087, 1180, 1313
BssT1I CCWWGG 3 cut(s) 312, 695, 984
Bst2UI CCWGG 2 cut(s) 853, 1043
Bst4CI ACNGT 4 cut(s) 18, 703, 782, 821
Bst6I CTCTTC 6 cut(s) 180, 281, 387, 524, 671, 1110
BstAPI GCANNNNNTGC 2 cut(s) 242, 728
BstAUI TGTACA 1 cut(s) 78
BstC8I GCNNGC 6 cut(s) 91, 180, 267, 473, 795, 1023
BstDEI CTNAG 9 cut(s) 111, 202, 560, 592, 609, 652, 1160, 1203, 1217
BstDSI CCRYGG 1 cut(s) 695
BstF5I GGATG 4 cut(s) 34, 258, 574, 623
BstFNI CGCG 2 cut(s) 250, 1076
BstHHI GCGC 2 cut(s) 159, 250
BstKTI GATC 4 cut(s) 787, 1090, 1183, 1316
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 4 cut(s) 784, 1087, 1180, 1313
BstNI CCWGG 2 cut(s) 853, 1043
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 4 cut(s) 851, 861, 933, 1041
BstSFI CTRYAG 3 cut(s) 720, 1092, 1347
BstSLI GKGCMC 1 cut(s) 780
BstUI CGCG 2 cut(s) 250, 1076
BstV1I GCAGC 7 cut(s) 178, 220, 257, 551, 706, 917, 920
BstV2I GAAGAC 1 cut(s) 663
BstX2I RGATCY 1 cut(s) 1180
BstXI CCANNNNNNTGG 1 cut(s) 696
BstYI RGATCY 1 cut(s) 1180
BsuRI GGCC 5 cut(s) 200, 471, 511, 738, 1108
BtgI CCRYGG 1 cut(s) 695
BtsCI GGATG 4 cut(s) 34, 258, 574, 623
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 2 cut(s) 732, 1101
BveI ACCTGC 1 cut(s) 611
Cac8I GCNNGC 6 cut(s) 91, 180, 267, 473, 795, 1023
CfoI GCGC 2 cut(s) 159, 250
Cfr10I RCCGGY 4 cut(s) 660, 793, 911, 1212
Cfr13I GGNCC 2 cut(s) 455, 1210
CsiI ACCWGGT 1 cut(s) 1041
Csp6I GTAC 6 cut(s) 79, 600, 634, 915, 1068, 1301
CviAII CATG 5 cut(s) 76, 123, 583, 637, 696
CviQI GTAC 6 cut(s) 79, 600, 634, 915, 1068, 1301
DdeI CTNAG 9 cut(s) 111, 202, 560, 592, 609, 652, 1160, 1203, 1217
DpnI GATC 4 cut(s) 786, 1089, 1182, 1315
DpnII GATC 4 cut(s) 784, 1087, 1180, 1313
Eam1104I CTCTTC 6 cut(s) 180, 281, 387, 524, 671, 1110
EarI CTCTTC 6 cut(s) 180, 281, 387, 524, 671, 1110
Ecl136II GAGCTC 2 cut(s) 130, 1159
Eco130I CCWWGG 3 cut(s) 312, 695, 984
Eco147I AGGCCT 1 cut(s) 471
Eco24I GRGCYC 2 cut(s) 132, 1161
Eco31I GGTCTC 1 cut(s) 272
Eco47I GGWCC 2 cut(s) 455, 1210
Eco53kI GAGCTC 2 cut(s) 130, 1159
Eco57I CTGAAG 2 cut(s) 69, 204
EcoICRI GAGCTC 2 cut(s) 130, 1159
EcoRII CCWGG 2 cut(s) 851, 1041
EcoT14I CCWWGG 3 cut(s) 312, 695, 984
EcoT38I GRGCYC 2 cut(s) 132, 1161
ErhI CCWWGG 3 cut(s) 312, 695, 984
FaeI CATG 5 cut(s) 79, 126, 586, 640, 699
FalI AAGNNNNNCTT 4 cut(s) 162, 194, 459, 491
FaqI GGGAC 2 cut(s) 845, 1021
FatI CATG 5 cut(s) 75, 122, 582, 636, 695
Fnu4HI GCNGC 7 cut(s) 192, 234, 246, 565, 720, 906, 909
FokI GGATG 4 cut(s) 41, 265, 561, 630
FriOI GRGCYC 2 cut(s) 132, 1161
Fsp4HI GCNGC 7 cut(s) 192, 234, 246, 565, 720, 906, 909
FspBI CTAG 3 cut(s) 266, 809, 857
GlaI GCGC 2 cut(s) 158, 249
GluI GCNGC 7 cut(s) 192, 234, 246, 565, 720, 906, 909
HaeIII GGCC 5 cut(s) 200, 471, 511, 738, 1108
HapII CCGG 8 cut(s) 661, 794, 814, 863, 912, 935, 1178, 1213
HhaI GCGC 2 cut(s) 159, 250
Hin1II CATG 5 cut(s) 79, 126, 586, 640, 699
Hin6I GCGC 2 cut(s) 157, 248
HinP1I GCGC 2 cut(s) 157, 248
HindIII AAGCTT 3 cut(s) 99, 176, 649
HinfI GANTC 3 cut(s) 1006, 1052, 1231
HpaII CCGG 8 cut(s) 661, 794, 814, 863, 912, 935, 1178, 1213
HphI GGTGA 2 cut(s) 44, 743
Hpy166II GTNNAC 2 cut(s) 81, 778
Hpy188I TCNGA 3 cut(s) 945, 973, 1236
Hpy188III TCNNGA 3 cut(s) 505, 670, 1162
Hpy8I GTNNAC 2 cut(s) 81, 778
HpyAV CCTTC 7 cut(s) 112, 205, 327, 437, 667, 749, 1231
HpyCH4III ACNGT 4 cut(s) 18, 703, 782, 821
HpyF3I CTNAG 9 cut(s) 111, 202, 560, 592, 609, 652, 1160, 1203, 1217
Hsp92II CATG 5 cut(s) 79, 126, 586, 640, 699
HspAI GCGC 2 cut(s) 157, 248
KpnI GGTACC 1 cut(s) 603
KroI GCCGGC 1 cut(s) 793
KroNI GCCGGC 1 cut(s) 795
Kzo9I GATC 4 cut(s) 784, 1087, 1180, 1313
LguI GCTCTTC 1 cut(s) 671
LmnI GCTCC 2 cut(s) 127, 135
Lsp1109I GCAGC 7 cut(s) 178, 220, 257, 551, 706, 917, 920
LweI GCATC 5 cut(s) 116, 232, 608, 875, 1295
MabI ACCWGGT 1 cut(s) 1041
MaeI CTAG 3 cut(s) 266, 809, 857
MaeIII GTNAC 3 cut(s) 12, 883, 999
MalI GATC 4 cut(s) 786, 1089, 1182, 1315
MboI GATC 4 cut(s) 784, 1087, 1180, 1313
MboII GAAGA 8 cut(s) 197, 298, 404, 541, 658, 668, 740, 1127
MflI RGATCY 1 cut(s) 1180
MhlI GDGCHC 3 cut(s) 132, 780, 1161
MluCI AATT 5 cut(s) 56, 294, 536, 726, 990
MlyI GAGTC 1 cut(s) 1061
MmeI TCCRAC 1 cut(s) 565
MroNI GCCGGC 1 cut(s) 793
MseI TTAA 3 cut(s) 47, 420, 477
MspI CCGG 8 cut(s) 661, 794, 814, 863, 912, 935, 1178, 1213
MspR9I CCNGG 4 cut(s) 853, 863, 935, 1043
Mva1269I GAATGC 1 cut(s) 1276
MvaI CCWGG 2 cut(s) 853, 1043
MvnI CGCG 2 cut(s) 250, 1076
NaeI GCCGGC 1 cut(s) 795
NciI CCSGG 2 cut(s) 863, 935
NcoI CCATGG 1 cut(s) 695
NdeII GATC 4 cut(s) 784, 1087, 1180, 1313
NgoMIV GCCGGC 1 cut(s) 793
NheI GCTAGC 1 cut(s) 265
NlaIII CATG 5 cut(s) 79, 126, 586, 640, 699
NlaIV GGNNCC 1 cut(s) 601
NmuCI GTSAC 1 cut(s) 883
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 471
PciI ACATGT 1 cut(s) 75
PciSI GCTCTTC 1 cut(s) 671
PctI GAATGC 1 cut(s) 1276
PdiI GCCGGC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 1006, 1231
PkrI GCNGC 7 cut(s) 193, 235, 247, 566, 721, 907, 910
PleI GAGTC 1 cut(s) 1060
PpsI GAGTC 1 cut(s) 1060
PscI ACATGT 1 cut(s) 75
PsiI TTATAA 1 cut(s) 465
Psp124BI GAGCTC 2 cut(s) 132, 1161
Psp6I CCWGG 2 cut(s) 851, 1041
PspGI CCWGG 2 cut(s) 851, 1041
PspN4I GGNNCC 1 cut(s) 601
PspPI GGNCC 2 cut(s) 455, 1210
PstI CTGCAG 3 cut(s) 724, 1096, 1351
PsuI RGATCY 1 cut(s) 1180
RsaI GTAC 6 cut(s) 80, 601, 635, 916, 1069, 1302
RsaNI GTAC 6 cut(s) 79, 600, 634, 915, 1068, 1301
SacI GAGCTC 2 cut(s) 132, 1161
SapI GCTCTTC 1 cut(s) 671
SaqAI TTAA 3 cut(s) 47, 420, 477
SatI GCNGC 7 cut(s) 192, 234, 246, 565, 720, 906, 909
Sau3AI GATC 4 cut(s) 784, 1087, 1180, 1313
Sau96I GGNCC 2 cut(s) 455, 1210
SchI GAGTC 1 cut(s) 1061
ScrFI CCNGG 4 cut(s) 853, 863, 935, 1043
SduI GDGCHC 3 cut(s) 132, 780, 1161
SexAI ACCWGGT 1 cut(s) 1041
SfaNI GCATC 5 cut(s) 116, 232, 608, 875, 1295
SfcI CTRYAG 3 cut(s) 720, 1092, 1347
SinI GGWCC 2 cut(s) 455, 1210
SmlI CTYRAG 2 cut(s) 505, 788
SmoI CTYRAG 2 cut(s) 505, 788
Sse9I AATT 5 cut(s) 56, 294, 536, 726, 990
SseBI AGGCCT 1 cut(s) 471
SsiI CCGC 3 cut(s) 250, 895, 1076
SspMI CTAG 3 cut(s) 266, 809, 857
SstI GAGCTC 2 cut(s) 132, 1161
StuI AGGCCT 1 cut(s) 471
StyD4I CCNGG 4 cut(s) 851, 861, 933, 1041
StyI CCWWGG 3 cut(s) 312, 695, 984
TaaI ACNGT 4 cut(s) 18, 703, 782, 821
TaqI TCGA 1 cut(s) 1270
TasI AATT 5 cut(s) 56, 294, 536, 726, 990
TatI WGTACW 2 cut(s) 78, 633
TfiI GAWTC 2 cut(s) 1006, 1231
Tru1I TTAA 3 cut(s) 47, 420, 477
Tru9I TTAA 3 cut(s) 47, 420, 477
TscAI CASTG 2 cut(s) 739, 1101
TseFI GTSAC 1 cut(s) 883
TseI GCWGC 7 cut(s) 191, 233, 245, 564, 719, 905, 908
Tsp45I GTSAC 1 cut(s) 883
TspGWI ACGGA 2 cut(s) 660, 695
TspRI CASTG 2 cut(s) 739, 1101
VneI GTGCAC 1 cut(s) 776
VpaK11BI GGWCC 2 cut(s) 455, 1210
XceI RCATGY 1 cut(s) 79
XspI CTAG 3 cut(s) 266, 809, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.