MD09G1196100.v1.1

NADH ubiquinone oxidoreductase, 20 Kd subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0013283
Physical Location & Seq
Forward (+)
17865283 .. 17865744
462 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1196100.v1.1.491

Sequence Viewer

Length: 462 bp
ATGAAAATGGCTCCTTCTTTAGTAAGATTATATGAACAAATGCCTGAGCCAAAATATGTTATTGCTATGGGAGCATGCACAATTACAGAGGGGATGTTCAGTACCAATTCTTATAGTACTGTTCGGGGAGTTGATAAGTTAATTCCTGAGGATGTCTATTTGCCAGGCTGTCCACCTAAACCGGAGGCCATTATAGATGCTATAACAAAACTTTGTAAGAAAATTTCTCGAGAAATCTATGAAGATCGAATTAGATCTCAACAGGAAAATCAGTGTTTTACTACTACCCATAAGTTTCGTGTTAGACGCAGTACTCATACTGGAAATTATGATCAGGGATTACTCTATACTGGAAATTATGATCAGGGATTACTCTATCAACCACCGTCTACTTCAAAGATCCTTCTTGAAATATTTTTCAAATATAAAAGTTCAATAACTTTCTCTGAATTAGTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Oxidored_q6

Protein Analysis

154

Amino Acids

17.54

Weight (kDa)

8.62

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q6 PF01058 2 - 71 6.6e-19 NADH ubiquinone oxidoreductase, 20 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 389
AclWI GGATC 1 cut(s) 394
AcsI RAATTY 1 cut(s) 222
AfaI GTAC 3 cut(s) 103, 118, 313
AgsI TTSAA 4 cut(s) 396, 410, 421, 435
AjnI CCWGG 1 cut(s) 163
AlwI GGATC 1 cut(s) 394
Ama87I CYCGRG 1 cut(s) 228
AoxI GGCC 1 cut(s) 186
ApoI RAATTY 1 cut(s) 222
AvaI CYCGRG 1 cut(s) 228
AxyI CCTNAGG 1 cut(s) 147
BciT130I CCWGG 1 cut(s) 165
BclI TGATCA 2 cut(s) 331, 361
BglII AGATCT 1 cut(s) 254
BmcAI AGTACT 2 cut(s) 118, 313
Bme1390I CCNGG 1 cut(s) 165
BmeT110I CYCGRG 1 cut(s) 228
BmiI GGNNCC 1 cut(s) 12
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 181
Bse1I ACTGG 2 cut(s) 325, 355
Bse21I CCTNAGG 1 cut(s) 147
BseBI CCWGG 1 cut(s) 165
BseGI GGATG 2 cut(s) 99, 157
BseMII CTCAG 2 cut(s) 36, 138
BseNI ACTGG 2 cut(s) 325, 355
BshFI GGCC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 228
BsiSI CCGG 1 cut(s) 182
BsnI GGCC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 228
Bsp143I GATC 5 cut(s) 244, 254, 331, 361, 399
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 2 cut(s) 37, 139
BspLI GGNNCC 1 cut(s) 12
BspPI GGATC 1 cut(s) 394
BsrI ACTGG 2 cut(s) 325, 355
BssMI GATC 5 cut(s) 244, 254, 331, 361, 399
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 121, 387
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 2 cut(s) 45, 147
BstF5I GGATG 2 cut(s) 99, 157
BstKTI GATC 5 cut(s) 247, 257, 334, 364, 402
BstMBI GATC 5 cut(s) 244, 254, 331, 361, 399
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 1 cut(s) 78
BstSCI CCNGG 1 cut(s) 163
BstX2I RGATCY 2 cut(s) 254, 399
BstYI RGATCY 2 cut(s) 254, 399
Bsu36I CCTNAGG 1 cut(s) 147
BsuRI GGCC 1 cut(s) 188
BtsCI GGATG 2 cut(s) 99, 157
BtsIMutI CAGTG 1 cut(s) 278
Cac8I GCNNGC 1 cut(s) 76
CseI GACGC 1 cut(s) 315
Csp6I GTAC 3 cut(s) 102, 117, 312
CviAII CATG 1 cut(s) 75
CviJI RGCY 4 cut(s) 11, 49, 168, 188
CviKI_1 RGCY 4 cut(s) 11, 49, 168, 188
CviQI GTAC 3 cut(s) 102, 117, 312
DdeI CTNAG 2 cut(s) 45, 147
DpnI GATC 5 cut(s) 246, 256, 333, 363, 401
DpnII GATC 5 cut(s) 244, 254, 331, 361, 399
Eco81I CCTNAGG 1 cut(s) 147
Eco88I CYCGRG 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 163
FaeI CATG 1 cut(s) 78
FatI CATG 1 cut(s) 74
FbaI TGATCA 2 cut(s) 331, 361
FblI GTMKAC 1 cut(s) 389
FokI GGATG 2 cut(s) 106, 164
HaeIII GGCC 1 cut(s) 188
HapII CCGG 1 cut(s) 182
HgaI GACGC 1 cut(s) 315
Hin1II CATG 1 cut(s) 78
HpaII CCGG 1 cut(s) 182
Hpy166II GTNNAC 2 cut(s) 173, 390
Hpy188I TCNGA 1 cut(s) 448
Hpy188III TCNNGA 4 cut(s) 146, 228, 230, 407
Hpy8I GTNNAC 2 cut(s) 173, 390
HpyAV CCTTC 2 cut(s) 24, 413
HpyCH4III ACNGT 2 cut(s) 121, 387
HpyCH4V TGCA 1 cut(s) 78
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 2 cut(s) 45, 147
Hsp92II CATG 1 cut(s) 78
Ksp22I TGATCA 2 cut(s) 331, 361
Kzo9I GATC 5 cut(s) 244, 254, 331, 361, 399
LmnI GCTCC 2 cut(s) 16, 71
LweI GCATC 1 cut(s) 187
MalI GATC 5 cut(s) 246, 256, 333, 363, 401
MboI GATC 5 cut(s) 244, 254, 331, 361, 399
MboII GAAGA 1 cut(s) 254
MflI RGATCY 2 cut(s) 254, 399
MluCI AATT 9 cut(s) 81, 106, 141, 222, 249, 325, 355, 449, 457
MnlI CCTC 3 cut(s) 82, 142, 178
MseI TTAA 1 cut(s) 140
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 1 cut(s) 71
NdeII GATC 5 cut(s) 244, 254, 331, 361, 399
NlaIII CATG 1 cut(s) 78
NlaIV GGNNCC 1 cut(s) 12
NspI RCATGY 1 cut(s) 78
PaeI GCATGC 1 cut(s) 78
PaeR7I CTCGAG 1 cut(s) 228
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 12
PsuI RGATCY 2 cut(s) 254, 399
RsaI GTAC 3 cut(s) 103, 118, 313
RsaNI GTAC 3 cut(s) 102, 117, 312
SaqAI TTAA 1 cut(s) 140
Sau3AI GATC 5 cut(s) 244, 254, 331, 361, 399
ScaI AGTACT 2 cut(s) 118, 313
ScrFI CCNGG 1 cut(s) 165
SetI ASST 1 cut(s) 178
SfaNI GCATC 1 cut(s) 187
Sfr274I CTCGAG 1 cut(s) 228
SlaI CTCGAG 1 cut(s) 228
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
SphI GCATGC 1 cut(s) 78
Sse9I AATT 9 cut(s) 81, 106, 141, 222, 249, 325, 355, 449, 457
SspI AATATT 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 163
TaaI ACNGT 2 cut(s) 121, 387
TaqI TCGA 2 cut(s) 229, 247
TasI AATT 9 cut(s) 81, 106, 141, 222, 249, 325, 355, 449, 457
TatI WGTACW 2 cut(s) 116, 311
Tru1I TTAA 1 cut(s) 140
Tru9I TTAA 1 cut(s) 140
TscAI CASTG 1 cut(s) 278
TspDTI ATGAA 3 cut(s) 17, 48, 255
TspRI CASTG 1 cut(s) 278
XapI RAATTY 1 cut(s) 222
XceI RCATGY 1 cut(s) 78
XhoI CTCGAG 1 cut(s) 228
XmiI GTMKAC 1 cut(s) 389
ZrmI AGTACT 2 cut(s) 118, 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.