Rroxscaffold_6G00421860

NADH ubiquinone oxidoreductase, 20 Kd subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013283
Physical Location & Seq
Forward (+)
42895339 .. 42905437
10099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00421860.1

Sequence Viewer

Length: 561 bp
ATGAGTGAATCCATTGATTCCTTATTGAACGGCAGCGAGAAAGTCGGTAAGTTCCAGCCCCGGAGGGATAATATCAACCACTTCAATACCGATTCTTACAATACTGTTCGGGGAGTTGATAAGCTAATTCCTGTAGATGTCTATTTGCCGGACTGTCCACCTAAACCGAAGGCGATTATAGATGCTATAACAAAACTTCGTAAGAAAATCTCTCGACAAATCTATGAAGATCGAATTCGATCTCAACGGGGGAATAGTTCGAAAGCACCGTGTAAAGCGACAAAAGAACTTGTCTCAACCTCGCCGAACGGTCTGAGACACCAATCTCCCGTGCCCTTCAACTCCTCAACTAGCGAAGCTTCTTCCACCGAACTTCCCCACCAGCGTGCTTCTTCCACCACGAGCTGCAACAAGTTGTACCATCGGTACAGAAACCAGATCGCCGCCATCGAAACCCCCGCTACCACGGATCGATCTCCACGGGTCGACCAGCCACCACGAGATGAAGCTTCCGATTTGGTTGTCTTCCACCAAGTCGATGAGAGCAGCTGCTTTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Oxidored_q6

Protein Analysis

186

Amino Acids

20.88

Weight (kDa)

9.23

Isoelectric Point (pI)

56.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q6 PF01058 32 - 66 8.3e-07 NADH ubiquinone oxidoreductase, 20 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 486
AciI CCGC 2 cut(s) 444, 459
AclWI GGATC 1 cut(s) 477
AcsI RAATTY 1 cut(s) 234
AfaI GTAC 2 cut(s) 419, 428
AgsI TTSAA 3 cut(s) 28, 85, 340
AleI CACNNNNGTG 1 cut(s) 384
AluBI AGCT 5 cut(s) 124, 359, 405, 509, 549
AluI AGCT 5 cut(s) 124, 359, 405, 509, 549
Alw26I GTCTC 2 cut(s) 298, 310
AlwI GGATC 1 cut(s) 477
ApeKI GCWGC 4 cut(s) 33, 405, 546, 549
ApoI RAATTY 1 cut(s) 234
AsuC2I CCSGG 1 cut(s) 61
AsuII TTCGAA 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 336
BauI CACGAG 2 cut(s) 400, 498
BbsI GAAGAC 1 cut(s) 517
BbvI GCAGC 3 cut(s) 45, 392, 536
BccI CCATC 2 cut(s) 429, 455
BceAI ACGGC 1 cut(s) 46
BcnI CCSGG 1 cut(s) 61
BcoDI GTCTC 2 cut(s) 298, 310
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 132
BisI GCNGC 5 cut(s) 34, 406, 444, 547, 550
BlsI GCNGC 5 cut(s) 35, 407, 445, 548, 551
Bme1390I CCNGG 1 cut(s) 61
BmrFI CCNGG 1 cut(s) 61
BmsI GCATC 1 cut(s) 172
BpiI GAAGAC 1 cut(s) 517
Bpu14I TTCGAA 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 61
Bsa29I ATCGAT 1 cut(s) 472
BsaJI CCNNGG 3 cut(s) 59, 465, 479
BseCI ATCGAT 1 cut(s) 472
BseDI CCNNGG 3 cut(s) 59, 465, 479
BseMII CTCAG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 334
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 3 cut(s) 45, 392, 536
BshVI ATCGAT 1 cut(s) 472
BsiSI CCGG 2 cut(s) 61, 149
BsmAI GTCTC 2 cut(s) 298, 310
Bsp119I TTCGAA 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 5 cut(s) 229, 239, 438, 469, 473
BspACI CCGC 2 cut(s) 444, 459
BspCNI CTCAG 1 cut(s) 306
BspDI ATCGAT 1 cut(s) 472
BspPI GGATC 1 cut(s) 477
BspT104I TTCGAA 1 cut(s) 260
BssECI CCNNGG 3 cut(s) 59, 465, 479
BssMI GATC 5 cut(s) 229, 239, 438, 469, 473
BssSI CACGAG 2 cut(s) 400, 498
Bst2BI CACGAG 2 cut(s) 400, 498
Bst4CI ACNGT 4 cut(s) 106, 155, 270, 311
BstBI TTCGAA 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 1 cut(s) 314
BstDSI CCRYGG 2 cut(s) 465, 479
BstKTI GATC 5 cut(s) 232, 242, 441, 472, 476
BstMAI GTCTC 2 cut(s) 298, 310
BstMBI GATC 5 cut(s) 229, 239, 438, 469, 473
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 1 cut(s) 132
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 3 cut(s) 45, 392, 536
BstV2I GAAGAC 1 cut(s) 517
Bsu15I ATCGAT 1 cut(s) 472
BsuTUI ATCGAT 1 cut(s) 472
BtgI CCRYGG 2 cut(s) 465, 479
Cac8I GCNNGC 1 cut(s) 387
ClaI ATCGAT 1 cut(s) 472
Csp6I GTAC 2 cut(s) 418, 427
CviJI RGCY 7 cut(s) 58, 124, 359, 405, 493, 509, 549
CviKI_1 RGCY 7 cut(s) 58, 124, 359, 405, 493, 509, 549
CviQI GTAC 2 cut(s) 418, 427
DdeI CTNAG 1 cut(s) 314
DpnI GATC 5 cut(s) 231, 241, 440, 471, 475
DpnII GATC 5 cut(s) 229, 239, 438, 469, 473
EcoRI GAATTC 1 cut(s) 234
FaiI YATR 3 cut(s) 179, 188, 225
FauI CCCGC 1 cut(s) 466
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 5 cut(s) 34, 406, 444, 547, 550
Fsp4HI GCNGC 5 cut(s) 34, 406, 444, 547, 550
FspBI CTAG 1 cut(s) 351
GluI GCNGC 5 cut(s) 34, 406, 444, 547, 550
HapII CCGG 2 cut(s) 61, 149
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HindIII AAGCTT 2 cut(s) 357, 507
HinfI GANTC 3 cut(s) 8, 17, 92
HpaII CCGG 2 cut(s) 61, 149
Hpy166II GTNNAC 2 cut(s) 158, 487
Hpy188I TCNGA 2 cut(s) 315, 514
Hpy188III TCNNGA 1 cut(s) 213
Hpy8I GTNNAC 2 cut(s) 158, 487
HpyAV CCTTC 2 cut(s) 163, 346
HpyCH4III ACNGT 4 cut(s) 106, 155, 270, 311
HpyCH4V TGCA 1 cut(s) 408
HpyF3I CTNAG 1 cut(s) 314
Kzo9I GATC 5 cut(s) 229, 239, 438, 469, 473
LpnPI CCDG 7 cut(s) 68, 74, 144, 162, 395, 449, 503
Lsp1109I GCAGC 3 cut(s) 45, 392, 536
LweI GCATC 1 cut(s) 172
MaeI CTAG 1 cut(s) 351
MalI GATC 5 cut(s) 231, 241, 440, 471, 475
MboI GATC 5 cut(s) 229, 239, 438, 469, 473
MboII GAAGA 4 cut(s) 239, 354, 384, 517
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 2 cut(s) 126, 234
MnlI CCTC 3 cut(s) 57, 310, 355
MslI CAYNNNNRTG 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 549
MspI CCGG 2 cut(s) 61, 149
MspR9I CCNGG 1 cut(s) 61
NciI CCSGG 1 cut(s) 61
NdeII GATC 5 cut(s) 229, 239, 438, 469, 473
NspV TTCGAA 1 cut(s) 260
OliI CACNNNNGTG 1 cut(s) 384
PcsI WCGNNNNNNNCGW 2 cut(s) 266, 447
PfeI GAWTC 3 cut(s) 8, 17, 92
PkrI GCNGC 5 cut(s) 35, 407, 445, 548, 551
PvuII CAGCTG 1 cut(s) 549
RsaI GTAC 2 cut(s) 419, 428
RsaNI GTAC 2 cut(s) 418, 427
RseI CAYNNNNRTG 1 cut(s) 384
SalI GTCGAC 1 cut(s) 485
SatI GCNGC 5 cut(s) 34, 406, 444, 547, 550
Sau3AI GATC 5 cut(s) 229, 239, 438, 469, 473
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 1 cut(s) 336
SetI ASST 7 cut(s) 126, 163, 302, 361, 407, 511, 551
SfaNI GCATC 1 cut(s) 172
SfcI CTRYAG 1 cut(s) 132
SfuI TTCGAA 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 384
Sse9I AATT 2 cut(s) 126, 234
SsiI CCGC 2 cut(s) 444, 459
SspMI CTAG 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 59
TaaI ACNGT 4 cut(s) 106, 155, 270, 311
TaqI TCGA 9 cut(s) 214, 232, 238, 260, 450, 472, 486, 537, 556
TasI AATT 2 cut(s) 126, 234
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 3 cut(s) 8, 17, 92
TseI GCWGC 4 cut(s) 33, 405, 546, 549
TspDTI ATGAA 2 cut(s) 240, 519
TspGWI ACGGA 1 cut(s) 482
XapI RAATTY 1 cut(s) 234
XmiI GTMKAC 1 cut(s) 486
XspI CTAG 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.