RLG00000000332

NDH shuttles electrons from NAD(P)H plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013283
Physical Location & Seq
Forward (+)
613984 .. 614664
681 bp
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UTR
Exon/CDS
Intron
RLM00000000332

Sequence Viewer

Length: 681 bp
ATGAATTCCATTATTGAATTTCCTTTACTTGATCGAACAACCCAAAATTCAGTTATTTCAACTACATCAAGTGATCTTTCAAATTGGTCAAGACTCTCCAGTTTATGGCCGCTTCTCTATGGTACCAGTTGTTGCTTCATTGAATTTGCTTCATTAATAGGCTCACGATTCGACTTTGATCGTTATGGACTGGTGCCACGATCTAGTCCGAGACAGGCGGACCTCATTTTAACAGCAGGCACTGTAACAATGAAAATGGCCCCTTCTTTAGTGAGATTATATGAGCAAATGCCTGAACCAAAATATGTTATTGCTATGGGAGCATGTACAATTACAGGAGGGATGTTCAGTACCGATTCTTACAGTACTGTTCGGGGAGTTGATAAGCTAATTCCTGTAGATGTCTATTTGCCGGGCTGTCCACCTAAACCGGAGGCGGTTATAGATGCTATAACAAAACTTCGTAAGAAAATCTCTCGAGAAATCTATGAAGATCGAATTCGATCTCAACAGGGGAATCGGTATTTTACTACCAGTCACAAGTTTCGTCTTGGACGTAGTACTCATACCGGAAATTATGATCAAGGATTGCTCTATGAACCACCCTCGACTTCAGAGATCCCTCCTGAAATATTTTTCAAATATAAAAGTTCAGTATCTTCCCACGAATTAGTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Oxidored_q4 Oxidored_q6

Protein Analysis

227

Amino Acids

25.42

Weight (kDa)

8.62

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q6 PF01058 44 - 154 6.6e-29 NADH ubiquinone oxidoreductase, 20 Kd subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 122
AccB1I GGYRCC 2 cut(s) 122, 193
AccB7I CCANNNNNTGG 1 cut(s) 105
AciI CCGC 3 cut(s) 110, 218, 437
AclWI GGATC 1 cut(s) 613
AcoI YGGCCR 1 cut(s) 107
AcsI RAATTY 5 cut(s) 4, 17, 46, 143, 498
AcuI CTGAAG 1 cut(s) 597
AfaI GTAC 5 cut(s) 124, 328, 352, 367, 562
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 5 cut(s) 17, 60, 81, 143, 640
AluBI AGCT 1 cut(s) 388
AluI AGCT 1 cut(s) 388
Alw26I GTCTC 1 cut(s) 205
AlwI GGATC 1 cut(s) 613
AlwNI CAGNNNCTG 1 cut(s) 242
Ama87I CYCGRG 1 cut(s) 477
AoxI GGCC 2 cut(s) 107, 258
ApoI RAATTY 5 cut(s) 4, 17, 46, 143, 498
AseI ATTAAT 1 cut(s) 155
Asp718I GGTACC 1 cut(s) 122
AspS9I GGNCC 2 cut(s) 220, 259
AsuC2I CCSGG 1 cut(s) 414
AvaI CYCGRG 1 cut(s) 477
AvaII GGWCC 1 cut(s) 220
BanI GGYRCC 2 cut(s) 122, 193
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 1 cut(s) 414
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 1 cut(s) 204
BfmI CTRYAG 1 cut(s) 396
BisI GCNGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 111
BmcAI AGTACT 2 cut(s) 367, 562
Bme1390I CCNGG 1 cut(s) 414
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 477
BmgT120I GGNCC 2 cut(s) 220, 259
BmiI GGNNCC 3 cut(s) 124, 195, 261
BmrFI CCNGG 1 cut(s) 414
BmsI GCATC 1 cut(s) 436
BpmI CTGGAG 1 cut(s) 82
BpuMI CCSGG 1 cut(s) 414
BsaWI WCCGGW 2 cut(s) 430, 569
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 4 cut(s) 99, 126, 195, 534
BseGI GGATG 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 105
BseNI ACTGG 4 cut(s) 99, 126, 195, 534
BshFI GGCC 2 cut(s) 109, 260
BshNI GGYRCC 2 cut(s) 122, 193
BsiHKCI CYCGRG 1 cut(s) 477
BsiSI CCGG 3 cut(s) 413, 431, 570
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 1 cut(s) 205
BsnI GGCC 2 cut(s) 109, 260
BsoBI CYCGRG 1 cut(s) 477
Bsp1407I TGTACA 1 cut(s) 326
Bsp143I GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
BspACI CCGC 3 cut(s) 110, 218, 437
BspANI GGCC 2 cut(s) 109, 260
BspLI GGNNCC 3 cut(s) 124, 195, 261
BspPI GGATC 1 cut(s) 613
BspT107I GGYRCC 2 cut(s) 122, 193
BsrGI TGTACA 1 cut(s) 326
BsrI ACTGG 4 cut(s) 99, 126, 195, 534
BssMI GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
Bst4CI ACNGT 3 cut(s) 244, 365, 370
BstAUI TGTACA 1 cut(s) 326
BstC8I GCNNGC 1 cut(s) 238
BstF5I GGATG 1 cut(s) 348
BstKTI GATC 8 cut(s) 34, 76, 181, 203, 496, 506, 583, 621
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstNSI RCATGY 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 412
BstSFI CTRYAG 1 cut(s) 396
BstX2I RGATCY 1 cut(s) 618
BstYI RGATCY 1 cut(s) 618
BsuRI GGCC 2 cut(s) 109, 260
BtsCI GGATG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 238
CaiI CAGNNNCTG 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 220, 259
Csp6I GTAC 5 cut(s) 123, 327, 351, 366, 561
CviAII CATG 1 cut(s) 324
CviJI RGCY 5 cut(s) 109, 162, 260, 388, 417
CviKI_1 RGCY 5 cut(s) 109, 162, 260, 388, 417
CviQI GTAC 5 cut(s) 123, 327, 351, 366, 561
DpnI GATC 8 cut(s) 33, 75, 180, 202, 495, 505, 582, 620
DpnII GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
EaeI YGGCCR 1 cut(s) 107
EciI GGCGGA 1 cut(s) 233
Eco47I GGWCC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 477
EcoRI GAATTC 2 cut(s) 4, 498
FaeI CATG 1 cut(s) 327
FatI CATG 1 cut(s) 323
FbaI TGATCA 1 cut(s) 580
Fnu4HI GCNGC 1 cut(s) 110
FokI GGATG 1 cut(s) 355
Fsp4HI GCNGC 1 cut(s) 110
FspBI CTAG 1 cut(s) 204
GluI GCNGC 1 cut(s) 110
GsuI CTGGAG 1 cut(s) 82
HaeIII GGCC 2 cut(s) 109, 260
HapII CCGG 3 cut(s) 413, 431, 570
Hin1II CATG 1 cut(s) 327
HinfI GANTC 4 cut(s) 93, 168, 356, 517
HpaII CCGG 3 cut(s) 413, 431, 570
Hpy166II GTNNAC 1 cut(s) 422
Hpy188I TCNGA 2 cut(s) 210, 616
Hpy188III TCNNGA 5 cut(s) 90, 165, 477, 479, 626
Hpy8I GTNNAC 1 cut(s) 422
HpyAV CCTTC 1 cut(s) 273
HpyCH4III ACNGT 3 cut(s) 244, 365, 370
HpyCH4IV ACGT 1 cut(s) 556
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpySE526I ACGT 1 cut(s) 556
Hsp92II CATG 1 cut(s) 327
KpnI GGTACC 1 cut(s) 126
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
LmnI GCTCC 1 cut(s) 320
LweI GCATC 1 cut(s) 436
MaeI CTAG 1 cut(s) 204
MaeII ACGT 1 cut(s) 556
MaeIII GTNAC 2 cut(s) 244, 536
MalI GATC 8 cut(s) 33, 75, 180, 202, 495, 505, 582, 620
MboI GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
MboII GAAGA 2 cut(s) 503, 651
MflI RGATCY 1 cut(s) 618
MlyI GAGTC 1 cut(s) 87
MnlI CCTC 5 cut(s) 233, 332, 427, 616, 633
MseI TTAA 2 cut(s) 155, 230
MspI CCGG 3 cut(s) 413, 431, 570
MspR9I CCNGG 1 cut(s) 414
MwoI GCNNNNNNNGC 1 cut(s) 320
NciI CCSGG 1 cut(s) 414
NdeII GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
NlaIII CATG 1 cut(s) 327
NlaIV GGNNCC 3 cut(s) 124, 195, 261
NmuCI GTSAC 1 cut(s) 536
NspI RCATGY 1 cut(s) 327
PaeR7I CTCGAG 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 553
PfeI GAWTC 3 cut(s) 168, 356, 517
PflMI CCANNNNNTGG 1 cut(s) 105
PkrI GCNGC 1 cut(s) 111
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 155
PspN4I GGNNCC 3 cut(s) 124, 195, 261
PspPI GGNCC 2 cut(s) 220, 259
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 1 cut(s) 618
RsaI GTAC 5 cut(s) 124, 328, 352, 367, 562
RsaNI GTAC 5 cut(s) 123, 327, 351, 366, 561
SaqAI TTAA 2 cut(s) 155, 230
SatI GCNGC 1 cut(s) 110
Sau3AI GATC 8 cut(s) 31, 73, 178, 200, 493, 503, 580, 618
Sau96I GGNCC 2 cut(s) 220, 259
ScaI AGTACT 2 cut(s) 367, 562
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 414
SetI ASST 4 cut(s) 225, 390, 427, 559
SfaNI GCATC 1 cut(s) 436
SfcI CTRYAG 1 cut(s) 396
Sfr274I CTCGAG 1 cut(s) 477
SinI GGWCC 1 cut(s) 220
SlaI CTCGAG 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 477
SmoI CTYRAG 1 cut(s) 477
SsiI CCGC 3 cut(s) 110, 218, 437
SspI AATATT 1 cut(s) 633
SspMI CTAG 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 412
TaaI ACNGT 3 cut(s) 244, 365, 370
TaiI ACGT 1 cut(s) 559
TaqI TCGA 6 cut(s) 34, 171, 478, 496, 502, 608
TatI WGTACW 3 cut(s) 326, 365, 560
TauI GCSGC 1 cut(s) 112
TfiI GAWTC 3 cut(s) 168, 356, 517
Tru1I TTAA 2 cut(s) 155, 230
Tru9I TTAA 2 cut(s) 155, 230
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 536
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 6 cut(s) 17, 127, 141, 266, 504, 612
TspRI CASTG 1 cut(s) 247
Van91I CCANNNNNTGG 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 220
VspI ATTAAT 1 cut(s) 155
XapI RAATTY 5 cut(s) 4, 17, 46, 143, 498
XceI RCATGY 1 cut(s) 327
XhoI CTCGAG 1 cut(s) 477
XspI CTAG 1 cut(s) 204
ZrmI AGTACT 2 cut(s) 367, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.