Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0015135

Physical Location & Seq

Genomic Coordinates Reverse (-)

20217930 .. 20225067

Gene Structure 7138 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1209900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGTCCAGATCAAAACCCACCTCCAGATTCATCATTGTTGTCCTCACTCTCATCCTCACCCTCCTACTCCATTTCCCGACCCCCATCATATCCTCTGAACCCCAAAACCTCACCGCAGACGAAACCGAAGATGGCGAACTTGAAGAGCTCCTGGCGCCCACCACAAGGTCCTCGGAGGCCGAGCTTTTATCCAATGCACAAAGGATTGTTCTGGAGCTTAATCATGACAATACCAAGAGGGTTATTGAGAACAATGAGTTTGTTCTGGTTCTTGGTTATACTCCTTGGTGTTCCAGGAGTGCTGAGCTGATGCCTCAGTTTGCTGAGGCTGCCACTGCGCTGAAGGAATTGGGGAGCCCCCTTTTGATGGCGAAGATTGATGCCGAGCGGCACGCGAAAACTGCCTCGTCTTTGGAGATCAAAGGGTTCCCTACTCTGCTTCTGTTTGTCAATGGCACCTCCCAAGTGTACACTGGTGGATTTTCTGCGGAAGAAATAGTGATATGGGCGAGGAAGAAAACTGGTGAACCTGTTATTAGGATAAGCTCGGTGACAGAGGCTGAAGAATTTCTCAAGAAGTCTGACATATTTGTTGTTGGTTTGTTTGAAAAGTTTGAGGGGCCTAACCACAAAGAGTTTGTAAATGCAGCGACAGCTGACAATGCAATCCAGTTTGTAGAAGCAAGCAACATCGAGGTTGCTAACGTTCTCTTTCCAAATATGAAACCAACTAGCTTTTTCCTTGGAATTGTGAAAAGTGAGCCAGAAAGATGCACTGCATATGAGGGGACCTTTAGAGCGGATGAAATACTGCAGTTTCTTGACCATAACAAGTTTCCGTTAGTTAACAGACTTACTGAAGCGAATTCTGCAAAAGTGTACTCCAGCCCTATTCAAATTCAGGTTCATGTTTTTGCAGACGCAGATGACTTCAAGAAACTTCTTGAGCCTCTTCAAGATGTTGCTAGACAGTTCAAGTCAAAGATACTGTTTATATTAATAGACATTACGGACGAAAACCTTGCTAAGCCTTACCTAACGGTATTTGGGCTTGAAGAATCAAACAGCACTGTGGTCATTGCTTTTGATATCAGAGGGAACTCTAAATATCTGTTGGAATCAGATCCAACACAAAGCAATCTTGAAGAGTTCTGCTCAGGGCTTCTACAGGGTACTGTATCTCCACACTTCAAATCGCAACTAATACCTGATAATAAGAATGAAACGGTCCTGAGCGTTGTAGGAAAGACATTGGATGACTTGGTTTTAAATAGTCACAAGAATGTTGTACTAGAGACCACGGCTAAGCATGTACAAAAGTTGGCTAAGCACTTTAAAAGTTTGGATAATCTACTCTTTGCAAGAATAGATGCGTCTGCGAATGAACATCCGAGACTGCAAGCAAGCGACTACCCAACGCTCTTGTTATACACAGCCATCGATAAAGCCAACCCGATCAAACTTTCTACAAAATCGAGTTTGAAGGACGTGGCCGCAACCATCAACAAACATTTGAAAGCCAAAGATAACGCCGCTAAAGGTGAGTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003756 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006457 GO:0006950 GO:0008150 GO:0009987 GO:0012505 GO:0016853 GO:0016860 GO:0016864 GO:0033554 GO:0034976 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051716 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Thioredoxin Thioredoxin_6

Protein Analysis

517

Amino Acids

57.29

Weight (kDa)

5.46

Isoelectric Point (pI)

34.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calsequestrin	PF01216	70 - 350	2.5e-08	Calsequestrin
Thioredoxin	PF00085	71 - 169	1.2e-14	Thioredoxin
Thioredoxin_6	PF13848	206 - 386	5.6e-14	Thioredoxin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015135)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16110
fragaria_vesca	FvH4_6g31670
malus_domestica	MD09G1209900.v1.1 MD17G1191800.v1.1
prunus_persica	Prupe.3G067900_v2.0.a1
pyrus_communis	pycom17g19880
rosa_chinensis	RchiOBHm_Chr2g0138751
rosa_laevigata	RLG00000019758
rosa_multiflora	Rmu_sc0006243.1_g000002
rosa_roxburghii	Rroxscaffold_2G00105970
rosa_rugosa	Rorug02G0352600
rosa_samantha	Rh2AG402200 Rh2BG405400 Rh2CG388400 Rh2DG422400
rosa_wichuraiana	Rw2G032700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	154, 455
AccBSI	CCGCTC	2 cut(s)	388, 800
AccII	CGCG	1 cut(s)	395
AciI	CCGC	6 cut(s)	114, 388, 488, 800, 1494, 1533
AclI	AACGTT	1 cut(s)	705
AclWI	GGATC	1 cut(s)	1118
AcoI	YGGCCR	1 cut(s)	1491
AcsI	RAATTY	3 cut(s)	566, 865, 897
AcuI	CTGAAG	3 cut(s)	362, 582, 879
AcyI	GRCGYC	1 cut(s)	155
AfaI	GTAC	5 cut(s)	470, 881, 1174, 1290, 1314
AfiI	CCNNNNNNNGG	2 cut(s)	165, 367
AjiI	CACGTC	1 cut(s)	1489
AjnI	CCWGG	2 cut(s)	150, 293
AluBI	AGCT	7 cut(s)	148, 184, 217, 307, 546, 656, 735
AluI	AGCT	7 cut(s)	148, 184, 217, 307, 546, 656, 735
Alw21I	GWGCWC	1 cut(s)	150
Alw26I	GTCTC	2 cut(s)	1289, 1387
AlwI	GGATC	1 cut(s)	1118
AlwNI	CAGNNNCTG	1 cut(s)	560
AoxI	GGCC	3 cut(s)	177, 620, 1491
ApeKI	GCWGC	2 cut(s)	329, 647
ApoI	RAATTY	3 cut(s)	566, 865, 897
AseI	ATTAAT	1 cut(s)	998
Asp700I	GAANNNNTTC	1 cut(s)	567
AspLEI	GCGC	2 cut(s)	157, 340
AspS9I	GGNCC	4 cut(s)	168, 620, 789, 1228
AsuHPI	GGTGA	4 cut(s)	49, 103, 536, 562
AvaII	GGWCC	3 cut(s)	168, 789, 1228
BanI	GGYRCC	2 cut(s)	154, 455
BanII	GRGCYC	2 cut(s)	150, 359
Bbv12I	GWGCWC	1 cut(s)	150
BbvCI	CCTCAGC	1 cut(s)	324
BbvI	GCAGC	2 cut(s)	316, 659
BccI	CCATC	5 cut(s)	92, 125, 361, 1445, 1508
BceAI	ACGGC	1 cut(s)	1317
BcgI	CGANNNNNNTGC	2 cut(s)	1004, 1038
BciT130I	CCWGG	2 cut(s)	152, 295
BcoDI	GTCTC	2 cut(s)	1289, 1387
BfaI	CTAG	3 cut(s)	732, 966, 1292
BfmI	CTRYAG	2 cut(s)	812, 1166
BfoI	RGCGCY	1 cut(s)	158
BisI	GCNGC	5 cut(s)	330, 389, 648, 1494, 1533
BlpI	GCTNAGC	4 cut(s)	303, 1026, 1305, 1326
BlsI	GCNGC	5 cut(s)	331, 390, 649, 1495, 1534
Bme1390I	CCNGG	2 cut(s)	152, 295
Bme18I	GGWCC	3 cut(s)	168, 789, 1228
BmgBI	CACGTC	1 cut(s)	1489
BmgT120I	GGNCC	4 cut(s)	168, 620, 789, 1228
BmiI	GGNNCC	6 cut(s)	156, 356, 428, 457, 621, 790
BmrFI	CCNGG	2 cut(s)	152, 295
BmsI	GCATC	4 cut(s)	300, 370, 761, 1360
BplI	GAGNNNNNCTC	2 cut(s)	1139, 1171
BpmI	CTGGAG	3 cut(s)	7, 233, 868
Bpu10I	CCTNAGC	3 cut(s)	324, 1156, 1232
Bpu1102I	GCTNAGC	4 cut(s)	303, 1026, 1305, 1326
BpuEI	CTTGAG	2 cut(s)	557, 965
Bsa29I	ATCGAT	1 cut(s)	1440
BsaHI	GRCGYC	1 cut(s)	155
BsaI	GGTCTC	1 cut(s)	1289
BsaJI	CCNNGG	4 cut(s)	171, 284, 742, 1299
BsaXI	ACNNNNNCTCC	2 cut(s)	1165, 1195
Bsc4I	CCNNNNNNNGG	2 cut(s)	165, 367
Bse1I	ACTGG	3 cut(s)	478, 526, 670
Bse3DI	GCAATG	1 cut(s)	1077
BseBI	CCWGG	2 cut(s)	152, 295
BseCI	ATCGAT	1 cut(s)	1440
BseDI	CCNNGG	4 cut(s)	171, 284, 742, 1299
BseGI	GGATG	4 cut(s)	51, 808, 1261, 1387
BseLI	CCNNNNNNNGG	2 cut(s)	165, 367
BseMI	GCAATG	1 cut(s)	1077
BseMII	CTCAG	5 cut(s)	294, 315, 329, 1170, 1223
BseNI	ACTGG	3 cut(s)	478, 526, 670
BseXI	GCAGC	2 cut(s)	316, 659
Bsh1236I	CGCG	1 cut(s)	395
BshFI	GGCC	3 cut(s)	179, 622, 1493
BshNI	GGYRCC	2 cut(s)	154, 455
BshVI	ATCGAT	1 cut(s)	1440
BsiHKAI	GWGCWC	1 cut(s)	150
BslFI	GGGAC	1 cut(s)	802
BslI	CCNNNNNNNGG	2 cut(s)	165, 367
BsmAI	GTCTC	2 cut(s)	1289, 1387
BsmFI	GGGAC	1 cut(s)	802
BsnI	GGCC	3 cut(s)	179, 622, 1493
Bso31I	GGTCTC	1 cut(s)	1289
Bsp1286I	GDGCHC	2 cut(s)	150, 359
Bsp1407I	TGTACA	2 cut(s)	468, 1312
Bsp143I	GATC	4 cut(s)	8, 417, 1123, 1455
Bsp1720I	GCTNAGC	4 cut(s)	303, 1026, 1305, 1326
BspACI	CCGC	6 cut(s)	114, 388, 488, 800, 1494, 1533
BspANI	GGCC	3 cut(s)	179, 622, 1493
BspCNI	CTCAG	5 cut(s)	295, 316, 328, 1169, 1224
BspDI	ATCGAT	1 cut(s)	1440
BspFNI	CGCG	1 cut(s)	395
BspHI	TCATGA	1 cut(s)	223
BspLI	GGNNCC	6 cut(s)	156, 356, 428, 457, 621, 790
BspMAI	CTGCAG	1 cut(s)	816
BspPI	GGATC	1 cut(s)	1118
BspQI	GCTCTTC	1 cut(s)	138
BspT107I	GGYRCC	2 cut(s)	154, 455
BspTNI	GGTCTC	1 cut(s)	1289
BsrBI	CCGCTC	2 cut(s)	388, 800
BsrDI	GCAATG	1 cut(s)	1077
BsrGI	TGTACA	2 cut(s)	468, 1312
BsrI	ACTGG	3 cut(s)	478, 526, 670
BssECI	CCNNGG	4 cut(s)	171, 284, 742, 1299
BssMI	GATC	4 cut(s)	8, 417, 1123, 1455
BssNI	GRCGYC	1 cut(s)	155
BssT1I	CCWWGG	2 cut(s)	284, 742
Bst2UI	CCWGG	2 cut(s)	152, 295
Bst4CI	ACNGT	6 cut(s)	972, 990, 1042, 1072, 1177, 1228
Bst6I	CTCTTC	3 cut(s)	138, 957, 1140
BstACI	GRCGYC	1 cut(s)	155
BstAUI	TGTACA	2 cut(s)	468, 1312
BstC8I	GCNNGC	4 cut(s)	393, 685, 1401, 1405
BstDEI	CTNAG	8 cut(s)	303, 315, 324, 1026, 1156, 1232, 1305, 1326
BstDSI	CCRYGG	1 cut(s)	1299
BstF5I	GGATG	4 cut(s)	51, 808, 1261, 1387
BstFNI	CGCG	1 cut(s)	395
BstH2I	RGCGCY	1 cut(s)	158
BstHHI	GCGC	2 cut(s)	157, 340
BstKTI	GATC	4 cut(s)	11, 420, 1126, 1458
BstMAI	GTCTC	2 cut(s)	1289, 1387
BstMBI	GATC	4 cut(s)	8, 417, 1123, 1455
BstMWI	GCNNNNNNNGC	7 cut(s)	154, 329, 335, 401, 653, 662, 869
BstNI	CCWGG	2 cut(s)	152, 295
BstNSI	RCATGY	1 cut(s)	1313
BstSCI	CCNGG	2 cut(s)	150, 293
BstSFI	CTRYAG	2 cut(s)	812, 1166
BstUI	CGCG	1 cut(s)	395
BstV1I	GCAGC	2 cut(s)	316, 659
BstX2I	RGATCY	1 cut(s)	1123
BstYI	RGATCY	1 cut(s)	1123
Bsu15I	ATCGAT	1 cut(s)	1440
BsuRI	GGCC	3 cut(s)	179, 622, 1493
BsuTUI	ATCGAT	1 cut(s)	1440
BtgI	CCRYGG	1 cut(s)	1299
BtrI	CACGTC	1 cut(s)	1489
BtsCI	GGATG	4 cut(s)	51, 808, 1261, 1387
BtsI	GCAGTG	2 cut(s)	333, 774
BtsIMutI	CAGTG	4 cut(s)	333, 471, 774, 1068
Cac8I	GCNNGC	4 cut(s)	393, 685, 1401, 1405
CaiI	CAGNNNCTG	1 cut(s)	560
CciI	TCATGA	1 cut(s)	223
CfoI	GCGC	2 cut(s)	157, 340
Cfr13I	GGNCC	4 cut(s)	168, 620, 789, 1228
ClaI	ATCGAT	1 cut(s)	1440
CseI	GACGC	2 cut(s)	929, 1362
Csp6I	GTAC	5 cut(s)	469, 880, 1173, 1289, 1313
CviAII	CATG	3 cut(s)	224, 908, 1310
CviQI	GTAC	5 cut(s)	469, 880, 1173, 1289, 1313
DdeI	CTNAG	8 cut(s)	303, 315, 324, 1026, 1156, 1232, 1305, 1326
DinI	GGCGCC	1 cut(s)	156
DpnI	GATC	4 cut(s)	10, 419, 1125, 1457
DpnII	GATC	4 cut(s)	8, 417, 1123, 1455
DraI	TTTAAA	2 cut(s)	1269, 1336
EaeI	YGGCCR	1 cut(s)	1491
Eam1104I	CTCTTC	3 cut(s)	138, 957, 1140
EarI	CTCTTC	3 cut(s)	138, 957, 1140
Ecl136II	GAGCTC	1 cut(s)	148
Eco130I	CCWWGG	2 cut(s)	284, 742
Eco24I	GRGCYC	2 cut(s)	150, 359
Eco31I	GGTCTC	1 cut(s)	1289
Eco32I	GATATC	1 cut(s)	1090
Eco47I	GGWCC	3 cut(s)	168, 789, 1228
Eco53kI	GAGCTC	1 cut(s)	148
Eco57I	CTGAAG	3 cut(s)	362, 582, 879
EcoICRI	GAGCTC	1 cut(s)	148
EcoO109I	RGGNCCY	3 cut(s)	168, 620, 789
EcoRI	GAATTC	1 cut(s)	865
EcoRII	CCWGG	2 cut(s)	150, 293
EcoRV	GATATC	1 cut(s)	1090
EcoT14I	CCWWGG	2 cut(s)	284, 742
EcoT38I	GRGCYC	2 cut(s)	150, 359
EgeI	GGCGCC	1 cut(s)	156
EheI	GGCGCC	1 cut(s)	156
ErhI	CCWWGG	2 cut(s)	284, 742
FaeI	CATG	3 cut(s)	227, 911, 1313
FaqI	GGGAC	1 cut(s)	802
FatI	CATG	3 cut(s)	223, 907, 1309
FauNDI	CATATG	1 cut(s)	781
Fnu4HI	GCNGC	5 cut(s)	330, 389, 648, 1494, 1533
FokI	GGATG	4 cut(s)	38, 815, 1268, 1374
FriOI	GRGCYC	2 cut(s)	150, 359
Fsp4HI	GCNGC	5 cut(s)	330, 389, 648, 1494, 1533
FspBI	CTAG	3 cut(s)	732, 966, 1292
GlaI	GCGC	2 cut(s)	156, 339
GluI	GCNGC	5 cut(s)	330, 389, 648, 1494, 1533
GsuI	CTGGAG	3 cut(s)	7, 233, 868
HaeII	RGCGCY	1 cut(s)	158
HaeIII	GGCC	3 cut(s)	179, 622, 1493
HgaI	GACGC	2 cut(s)	929, 1362
HhaI	GCGC	2 cut(s)	157, 340
Hin1I	GRCGYC	1 cut(s)	155
Hin1II	CATG	3 cut(s)	227, 911, 1313
Hin6I	GCGC	2 cut(s)	155, 338
HinP1I	GCGC	2 cut(s)	155, 338
HincII	GTYRAC	1 cut(s)	847
HindII	GTYRAC	1 cut(s)	847
HinfI	GANTC	3 cut(s)	27, 1058, 1118
HpaI	GTTAAC	1 cut(s)	847
HphI	GGTGA	4 cut(s)	49, 103, 536, 562
Hpy166II	GTNNAC	5 cut(s)	469, 471, 527, 847, 880
Hpy188I	TCNGA	6 cut(s)	97, 175, 583, 1094, 1123, 1392
Hpy8I	GTNNAC	5 cut(s)	469, 471, 527, 847, 880
HpyAV	CCTTC	2 cut(s)	337, 1477
HpyCH4III	ACNGT	6 cut(s)	972, 990, 1042, 1072, 1177, 1228
HpyCH4IV	ACGT	2 cut(s)	705, 1488
HpyF10VI	GCNNNNNNNGC	7 cut(s)	154, 329, 335, 401, 653, 662, 869
HpyF3I	CTNAG	8 cut(s)	303, 315, 324, 1026, 1156, 1232, 1305, 1326
HpySE526I	ACGT	2 cut(s)	705, 1488
Hsp92I	GRCGYC	1 cut(s)	155
Hsp92II	CATG	3 cut(s)	227, 911, 1313
HspAI	GCGC	2 cut(s)	155, 338
KasI	GGCGCC	1 cut(s)	154
KspAI	GTTAAC	1 cut(s)	847
Kzo9I	GATC	4 cut(s)	8, 417, 1123, 1455
LguI	GCTCTTC	1 cut(s)	138
LmnI	GCTCC	3 cut(s)	153, 214, 354
Lsp1109I	GCAGC	2 cut(s)	316, 659
LweI	GCATC	4 cut(s)	300, 370, 761, 1360
MaeI	CTAG	3 cut(s)	732, 966, 1292
MaeII	ACGT	2 cut(s)	705, 1488
MaeIII	GTNAC	2 cut(s)	550, 1274
MalI	GATC	4 cut(s)	10, 419, 1125, 1457
MbiI	CCGCTC	2 cut(s)	388, 800
MboI	GATC	4 cut(s)	8, 417, 1123, 1455
MboII	GAAGA	9 cut(s)	140, 155, 385, 503, 526, 575, 944, 1067, 1157
MflI	RGATCY	1 cut(s)	1123
MhlI	GDGCHC	2 cut(s)	150, 359
MluCI	AATT	5 cut(s)	347, 566, 747, 865, 897
Mly113I	GGCGCC	1 cut(s)	155
MmeI	TCCRAC	2 cut(s)	1095, 1151
MroXI	GAANNNNTTC	1 cut(s)	567
MseI	TTAA	5 cut(s)	219, 846, 998, 1268, 1335
MslI	CAYNNNNRTG	1 cut(s)	1281
MspA1I	CMGCKG	1 cut(s)	656
MspR9I	CCNGG	2 cut(s)	152, 295
MvaI	CCWGG	2 cut(s)	152, 295
MvnI	CGCG	1 cut(s)	395
MwoI	GCNNNNNNNGC	7 cut(s)	154, 329, 335, 401, 653, 662, 869
NarI	GGCGCC	1 cut(s)	155
NdeI	CATATG	1 cut(s)	781
NdeII	GATC	4 cut(s)	8, 417, 1123, 1455
NlaIII	CATG	3 cut(s)	227, 911, 1313
NlaIV	GGNNCC	6 cut(s)	156, 356, 428, 457, 621, 790
NmeAIII	GCCGAG	2 cut(s)	205, 409
NmuCI	GTSAC	2 cut(s)	550, 1274
NspI	RCATGY	1 cut(s)	1313
PagI	TCATGA	1 cut(s)	223
PciSI	GCTCTTC	1 cut(s)	138
PdmI	GAANNNNTTC	1 cut(s)	567
PfeI	GAWTC	3 cut(s)	27, 1058, 1118
PfoI	TCCNGGA	1 cut(s)	293
PkrI	GCNGC	5 cut(s)	331, 390, 649, 1495, 1534
PluTI	GGCGCC	1 cut(s)	158
PpuMI	RGGWCCY	2 cut(s)	168, 789
PshBI	ATTAAT	1 cut(s)	998
Psp124BI	GAGCTC	1 cut(s)	150
Psp1406I	AACGTT	1 cut(s)	705
Psp5II	RGGWCCY	2 cut(s)	168, 789
Psp6I	CCWGG	2 cut(s)	150, 293
PspGI	CCWGG	2 cut(s)	150, 293
PspN4I	GGNNCC	6 cut(s)	156, 356, 428, 457, 621, 790
PspPI	GGNCC	4 cut(s)	168, 620, 789, 1228
PspPPI	RGGWCCY	2 cut(s)	168, 789
PstI	CTGCAG	1 cut(s)	816
PstNI	CAGNNNCTG	1 cut(s)	560
PsuI	RGATCY	1 cut(s)	1123
PvuII	CAGCTG	1 cut(s)	656
RsaI	GTAC	5 cut(s)	470, 881, 1174, 1290, 1314
RsaNI	GTAC	5 cut(s)	469, 880, 1173, 1289, 1313
RseI	CAYNNNNRTG	1 cut(s)	1281
SacI	GAGCTC	1 cut(s)	150
SapI	GCTCTTC	1 cut(s)	138
SaqAI	TTAA	5 cut(s)	219, 846, 998, 1268, 1335
SatI	GCNGC	5 cut(s)	330, 389, 648, 1494, 1533
Sau3AI	GATC	4 cut(s)	8, 417, 1123, 1455
Sau96I	GGNCC	4 cut(s)	168, 620, 789, 1228
ScrFI	CCNGG	2 cut(s)	152, 295
SduI	GDGCHC	2 cut(s)	150, 359
SfaNI	GCATC	4 cut(s)	300, 370, 761, 1360
SfcI	CTRYAG	2 cut(s)	812, 1166
SfoI	GGCGCC	1 cut(s)	156
SinI	GGWCC	3 cut(s)	168, 789, 1228
SmiMI	CAYNNNNRTG	1 cut(s)	1281
SmlI	CTYRAG	2 cut(s)	572, 944
SmoI	CTYRAG	2 cut(s)	572, 944
Sse9I	AATT	5 cut(s)	347, 566, 747, 865, 897
SsiI	CCGC	6 cut(s)	114, 388, 488, 800, 1494, 1533
SspDI	GGCGCC	1 cut(s)	154
SspMI	CTAG	3 cut(s)	732, 966, 1292
SstI	GAGCTC	1 cut(s)	150
StyD4I	CCNGG	2 cut(s)	150, 293
StyI	CCWWGG	2 cut(s)	284, 742
TaaI	ACNGT	6 cut(s)	972, 990, 1042, 1072, 1177, 1228
TaiI	ACGT	2 cut(s)	708, 1491
TaqI	TCGA	3 cut(s)	693, 1440, 1475
TasI	AATT	5 cut(s)	347, 566, 747, 865, 897
TatI	WGTACW	4 cut(s)	468, 879, 1288, 1312
TauI	GCSGC	3 cut(s)	391, 1496, 1535
TfiI	GAWTC	3 cut(s)	27, 1058, 1118
Tru1I	TTAA	5 cut(s)	219, 846, 998, 1268, 1335
Tru9I	TTAA	5 cut(s)	219, 846, 998, 1268, 1335
TscAI	CASTG	4 cut(s)	340, 478, 781, 1075
TseFI	GTSAC	2 cut(s)	550, 1274
TseI	GCWGC	2 cut(s)	329, 647
Tsp45I	GTSAC	2 cut(s)	550, 1274
TspDTI	ATGAA	6 cut(s)	19, 737, 819, 896, 1236, 1398
TspGWI	ACGGA	2 cut(s)	828, 1025
TspRI	CASTG	4 cut(s)	340, 478, 781, 1075
VpaK11BI	GGWCC	3 cut(s)	168, 789, 1228
VspI	ATTAAT	1 cut(s)	998
XapI	RAATTY	3 cut(s)	566, 865, 897
XceI	RCATGY	1 cut(s)	1313
XcmI	CCANNNNNNNNNTGG	1 cut(s)	470
XmnI	GAANNNNTTC	1 cut(s)	567
XspI	CTAG	3 cut(s)	732, 966, 1292

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.