Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0015135

Physical Location & Seq

Genomic Coordinates Reverse (-)

22857942 .. 22864982

Gene Structure 7041 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1191800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGTCAAGACCAAAACCCACCTCCAGATTCATCCTCTTCACCCTCACCCTCCTCCTCCTATTCCATTTCCCAACCCCCATCAAATCCTCCGAACCCCAAAACCCCGCCGCCGACGAAACCGAAGACGGCGAACTGGAAGAGCTCCTGGCATTGGACGAACAAGTAGAACAAGAAGAAGACCAAGACGGTGGGCCCACCACAAGGTCCTCGGAGGCCGAGCTTTTATCCAAAGCACAGAGGATTGTTCTGGAGCTTAATCATGACAATACAAAGAGGGTGATTGAGAACAATGAGTATGTTCTGGTTCTCGGGTACGCTCCCTGGTGTTCAAGGAGTGCTGAGCTGATGCCTCAGTTTGCTGAGGCTGCCACTGCGCTGAAGGAATTGGGGAGCCCTCTTTTAATGGCCAAGCTTGATGCCGAGCGGCACGCGAAAACCGCGTCGTCTCTCGAGATCAAAGGGTTCCCTACTCTGCTTCTGTTTGTCAATGGCACCTCCCAAGCGTACACTGGTGGATTTTCTGCGGAAGAAATAGTGACATGGGCGAGGAAGAAAACCGGTGAACCTGTTATTAGGATAAGCTCGGTGACAGAGGCAGCAGAATTTCTCAAGAAGTCCGACATATTTGTTCTTGGTGTGTTTGAAAATTTTGAGGGGCCCGAGCACAAAGAATTTGTAAAAGCAGCGGCAGCTGACAGTGCCATCCAGTTCGTAGAAGTAAGCAACATTGAGGTTGCTAATGTTCTCTTTCAAAATGTAAAACCAACTAATGGTTTCCTTGGAATTGTGAAAAGTGAGCCAGAAAGATACACTGCATATGAAGGGACCTTTAGAGTGGATGAAATATTGCAGTTTCTGGACTATAACAAGTTTCCATTAGTTAACAGACTTACTGAAGCAAATTCTGCAAAAGTGTACTCCAGTCCTATTCAACTTCAGGTTCATGTTTTTGCAGACGCGGATGACTTCAAGAAACTTCTTGAGCCCCTTCAAGATGTTGCTAGACAGTTCAAGTCAAAGATACTGTTCATATATATAGACATTACAGATGAAAATCTTGCTAAGCCTTACTTAACAGTATATGGGCTTGAAGAATCAAACAGTGCTGTGGTCACTGCTTTTGATATTAGAGGGAACTCTAAATATCTATTAGAATCAGATCCAACGCCAAGCAATCTTGAAGAGTTCTGCTCAGGCCTATTACATGGTACTGTATCTCCGCACTTCAAATCGCAACCAATACCTGATAACAAGAATGTAACCGTCCAGAGCATTGTAGGAAAGACATTTGATGACTTGGTTTTAAATAGTCACAAGAATGTGGTACTAGAGGTATACACACCATGGTGCATCACCTGTGAGACCACTGCCAAGCATGTAGAGAAGTTGGCTAAGCACTTTAAAAGTGTGGATAATCTACTCTTCGCAAGAATAGATGCGTCTGCAAATGAACATCCAAAACTGCAAGTAAGCGACTATCCAACACTCTTGCTGTACACAGCCAACGATAAAGGCAATCCGATCAAACTTTCTACAAAATCGAGCTTGAAGGACGTGGCTACAACTATCAACAAACATTTGAAAGCCAAGGATAACGCCGCTAAAGATGAATTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003756 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006457 GO:0006950 GO:0008150 GO:0009987 GO:0012505 GO:0016853 GO:0016860 GO:0016864 GO:0033554 GO:0034976 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051716 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

Thioredoxin Thioredoxin_6

Protein Analysis

539

Amino Acids

59.92

Weight (kDa)

5.13

Isoelectric Point (pI)

33.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calsequestrin	PF01216	82 - 358	6.1e-06	Calsequestrin
Thioredoxin	PF00085	83 - 183	6e-16	Thioredoxin
Thioredoxin_6	PF13848	214 - 398	1e-15	Thioredoxin-like domain
Thioredoxin	PF00085	423 - 521	2.7e-13	Thioredoxin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015135)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16110
fragaria_vesca	FvH4_6g31670
malus_domestica	MD09G1209900.v1.1 MD17G1191800.v1.1
prunus_persica	Prupe.3G067900_v2.0.a1
pyrus_communis	pycom17g19880
rosa_chinensis	RchiOBHm_Chr2g0138751
rosa_laevigata	RLG00000019758
rosa_multiflora	Rmu_sc0006243.1_g000002
rosa_roxburghii	Rroxscaffold_2G00105970
rosa_rugosa	Rorug02G0352600
rosa_samantha	Rh2AG402200 Rh2BG405400 Rh2CG388400 Rh2DG422400
rosa_wichuraiana	Rw2G032700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	491
AccB7I	CCANNNNNTGG	1 cut(s)	770
AccBSI	CCGCTC	1 cut(s)	424
AccI	GTMKAC	1 cut(s)	1335
AccII	CGCG	3 cut(s)	431, 440, 959
AciI	CCGC	9 cut(s)	105, 108, 424, 438, 524, 686, 959, 1220, 1599
AclWI	GGATC	1 cut(s)	1154
AcoI	YGGCCR	1 cut(s)	405
AcsI	RAATTY	4 cut(s)	602, 646, 671, 901
AcuI	CTGAAG	3 cut(s)	398, 915, 920
AfaI	GTAC	6 cut(s)	314, 506, 917, 1210, 1326, 1496
AfiI	CCNNNNNNNGG	3 cut(s)	151, 201, 770
AgeI	ACCGGT	1 cut(s)	557
AjiI	CACGTC	1 cut(s)	1555
AjnI	CCWGG	2 cut(s)	144, 320
AleI	CACNNNNGTG	1 cut(s)	1345
AluBI	AGCT	8 cut(s)	142, 220, 253, 343, 412, 582, 692, 1545
AluI	AGCT	8 cut(s)	142, 220, 253, 343, 412, 582, 692, 1545
Alw21I	GWGCWC	2 cut(s)	144, 666
Alw26I	GTCTC	2 cut(s)	450, 1355
AlwI	GGATC	1 cut(s)	1154
AlwNI	CAGNNNCTG	1 cut(s)	856
Ama87I	CYCGRG	3 cut(s)	308, 449, 659
AoxI	GGCC	5 cut(s)	191, 213, 405, 656, 1195
ApaI	GGGCCC	2 cut(s)	195, 660
ApeKI	GCWGC	4 cut(s)	365, 596, 683, 689
ApoI	RAATTY	4 cut(s)	602, 646, 671, 901
ArsI	GACNNNNNNTTYG	2 cut(s)	425, 457
AsiGI	ACCGGT	1 cut(s)	557
AspLEI	GCGC	1 cut(s)	376
AspS9I	GGNCC	6 cut(s)	191, 192, 204, 656, 657, 825
AsuHPI	GGTGA	6 cut(s)	31, 37, 289, 572, 598, 1345
AvaI	CYCGRG	3 cut(s)	308, 449, 659
AvaII	GGWCC	2 cut(s)	204, 825
BaeGI	GKGCMC	2 cut(s)	195, 660
BalI	TGGCCA	1 cut(s)	407
BanI	GGYRCC	1 cut(s)	491
BanII	GRGCYC	5 cut(s)	144, 195, 395, 660, 987
BbsI	GAAGAC	2 cut(s)	129, 183
Bbv12I	GWGCWC	2 cut(s)	144, 666
BbvCI	CCTCAGC	1 cut(s)	360
BbvI	GCAGC	4 cut(s)	352, 608, 695, 701
BccI	CCATC	2 cut(s)	86, 710
BceAI	ACGGC	1 cut(s)	142
BciT130I	CCWGG	2 cut(s)	146, 322
BcoDI	GTCTC	2 cut(s)	450, 1355
BfaI	CTAG	2 cut(s)	1002, 1328
BisI	GCNGC	8 cut(s)	108, 366, 425, 597, 684, 687, 690, 1599
BlpI	GCTNAGC	3 cut(s)	339, 1062, 1392
BlsI	GCNGC	8 cut(s)	109, 367, 426, 598, 685, 688, 691, 1600
Bme1390I	CCNGG	2 cut(s)	146, 322
Bme18I	GGWCC	2 cut(s)	204, 825
BmeT110I	CYCGRG	3 cut(s)	308, 449, 659
BmgBI	CACGTC	1 cut(s)	1555
BmgT120I	GGNCC	6 cut(s)	191, 192, 204, 656, 657, 825
BmiI	GGNNCC	7 cut(s)	193, 392, 464, 493, 657, 658, 826
BmrFI	CCNGG	2 cut(s)	146, 322
BmsI	GCATC	4 cut(s)	336, 406, 1359, 1426
BpiI	GAAGAC	2 cut(s)	129, 183
BplI	GAGNNNNNCTC	2 cut(s)	1175, 1207
BpmI	CTGGAG	3 cut(s)	7, 269, 904
Bpu10I	CCTNAGC	2 cut(s)	360, 1192
Bpu1102I	GCTNAGC	3 cut(s)	339, 1062, 1392
BpuEI	CTTGAG	2 cut(s)	593, 1001
BsaBI	GATNNNNATC	1 cut(s)	1053
BsaI	GGTCTC	1 cut(s)	1355
BsaJI	CCNNGG	5 cut(s)	207, 320, 778, 1343, 1587
BsaWI	WCCGGW	1 cut(s)	557
BsaXI	ACNNNNNCTCC	2 cut(s)	1201, 1231
Bsc4I	CCNNNNNNNGG	3 cut(s)	151, 201, 770
Bse118I	RCCGGY	1 cut(s)	557
Bse1I	ACTGG	4 cut(s)	138, 514, 706, 921
Bse8I	GATNNNNATC	1 cut(s)	1053
BseBI	CCWGG	2 cut(s)	146, 322
BseDI	CCNNGG	5 cut(s)	207, 320, 778, 1343, 1587
BseGI	GGATG	5 cut(s)	30, 702, 844, 967, 1453
BseJI	GATNNNNATC	1 cut(s)	1053
BseLI	CCNNNNNNNGG	3 cut(s)	151, 201, 770
BseMII	CTCAG	4 cut(s)	330, 351, 365, 1206
BseNI	ACTGG	4 cut(s)	138, 514, 706, 921
BseRI	GAGGAG	2 cut(s)	41, 44
BseSI	GKGCMC	2 cut(s)	195, 660
BseXI	GCAGC	4 cut(s)	352, 608, 695, 701
Bsh1236I	CGCG	3 cut(s)	431, 440, 959
BshFI	GGCC	5 cut(s)	193, 215, 407, 658, 1197
BshNI	GGYRCC	1 cut(s)	491
BshTI	ACCGGT	1 cut(s)	557
BsiHKAI	GWGCWC	2 cut(s)	144, 666
BsiHKCI	CYCGRG	3 cut(s)	308, 449, 659
BsiSI	CCGG	1 cut(s)	558
BslFI	GGGAC	1 cut(s)	838
BslI	CCNNNNNNNGG	3 cut(s)	151, 201, 770
BsmAI	GTCTC	2 cut(s)	450, 1355
BsmBI	CGTCTC	1 cut(s)	450
BsmFI	GGGAC	1 cut(s)	838
BsnI	GGCC	5 cut(s)	193, 215, 407, 658, 1197
Bso31I	GGTCTC	1 cut(s)	1355
BsoBI	CYCGRG	3 cut(s)	308, 449, 659
Bsp120I	GGGCCC	2 cut(s)	191, 656
Bsp1286I	GDGCHC	6 cut(s)	144, 195, 395, 660, 666, 987
Bsp1407I	TGTACA	1 cut(s)	1494
Bsp143I	GATC	3 cut(s)	453, 1159, 1521
Bsp1720I	GCTNAGC	3 cut(s)	339, 1062, 1392
Bsp19I	CCATGG	1 cut(s)	1343
BspACI	CCGC	9 cut(s)	105, 108, 424, 438, 524, 686, 959, 1220, 1599
BspANI	GGCC	5 cut(s)	193, 215, 407, 658, 1197
BspCNI	CTCAG	4 cut(s)	331, 352, 364, 1205
BspFNI	CGCG	3 cut(s)	431, 440, 959
BspHI	TCATGA	1 cut(s)	259
BspLI	GGNNCC	7 cut(s)	193, 392, 464, 493, 657, 658, 826
BspPI	GGATC	1 cut(s)	1154
BspQI	GCTCTTC	1 cut(s)	132
BspT107I	GGYRCC	1 cut(s)	491
BspTNI	GGTCTC	1 cut(s)	1355
BsrBI	CCGCTC	1 cut(s)	424
BsrFI	RCCGGY	1 cut(s)	557
BsrGI	TGTACA	1 cut(s)	1494
BsrI	ACTGG	4 cut(s)	138, 514, 706, 921
BssAI	RCCGGY	1 cut(s)	557
BssECI	CCNNGG	5 cut(s)	207, 320, 778, 1343, 1587
BssMI	GATC	3 cut(s)	453, 1159, 1521
BssNAI	GTATAC	1 cut(s)	1336
BssT1I	CCWWGG	3 cut(s)	778, 1343, 1587
Bst1107I	GTATAC	1 cut(s)	1336
Bst2UI	CCWGG	2 cut(s)	146, 322
Bst4CI	ACNGT	8 cut(s)	188, 698, 1008, 1026, 1078, 1103, 1213, 1264
Bst6I	CTCTTC	4 cut(s)	41, 132, 1176, 1427
BstAPI	GCANNNNNTGC	1 cut(s)	905
BstAUI	TGTACA	1 cut(s)	1494
BstC8I	GCNNGC	1 cut(s)	429
BstDEI	CTNAG	6 cut(s)	339, 351, 360, 1062, 1192, 1392
BstDSI	CCRYGG	1 cut(s)	1343
BstF5I	GGATG	5 cut(s)	30, 702, 844, 967, 1453
BstFNI	CGCG	3 cut(s)	431, 440, 959
BstHHI	GCGC	1 cut(s)	376
BstKTI	GATC	3 cut(s)	456, 1162, 1524
BstMAI	GTCTC	2 cut(s)	450, 1355
BstMBI	GATC	3 cut(s)	453, 1159, 1521
BstMWI	GCNNNNNNNGC	6 cut(s)	365, 371, 437, 689, 698, 905
BstNI	CCWGG	2 cut(s)	146, 322
BstNSI	RCATGY	1 cut(s)	1379
BstSCI	CCNGG	2 cut(s)	144, 320
BstSLI	GKGCMC	2 cut(s)	195, 660
BstUI	CGCG	3 cut(s)	431, 440, 959
BstV1I	GCAGC	4 cut(s)	352, 608, 695, 701
BstV2I	GAAGAC	2 cut(s)	129, 183
BstX2I	RGATCY	1 cut(s)	1159
BstXI	CCANNNNNNTGG	1 cut(s)	188
BstYI	RGATCY	1 cut(s)	1159
BstZ17I	GTATAC	1 cut(s)	1336
BsuRI	GGCC	5 cut(s)	193, 215, 407, 658, 1197
BtgI	CCRYGG	1 cut(s)	1343
BtrI	CACGTC	1 cut(s)	1555
BtsCI	GGATG	5 cut(s)	30, 702, 844, 967, 1453
BtsI	GCAGTG	4 cut(s)	369, 810, 1113, 1365
BtsIMutI	CAGTG	7 cut(s)	369, 507, 703, 810, 1108, 1113, 1365
Cac8I	GCNNGC	1 cut(s)	429
CaiI	CAGNNNCTG	1 cut(s)	856
CciI	TCATGA	1 cut(s)	259
CfoI	GCGC	1 cut(s)	376
Cfr10I	RCCGGY	1 cut(s)	557
Cfr13I	GGNCC	6 cut(s)	191, 192, 204, 656, 657, 825
CseI	GACGC	3 cut(s)	429, 965, 1428
Csp6I	GTAC	6 cut(s)	313, 505, 916, 1209, 1325, 1495
CspAI	ACCGGT	1 cut(s)	557
CviAII	CATG	6 cut(s)	260, 540, 944, 1205, 1344, 1376
CviQI	GTAC	6 cut(s)	313, 505, 916, 1209, 1325, 1495
DdeI	CTNAG	6 cut(s)	339, 351, 360, 1062, 1192, 1392
DpnI	GATC	3 cut(s)	455, 1161, 1523
DpnII	GATC	3 cut(s)	453, 1159, 1521
DraI	TTTAAA	2 cut(s)	1305, 1402
EaeI	YGGCCR	1 cut(s)	405
Eam1104I	CTCTTC	4 cut(s)	41, 132, 1176, 1427
EarI	CTCTTC	4 cut(s)	41, 132, 1176, 1427
Ecl136II	GAGCTC	1 cut(s)	142
Eco130I	CCWWGG	3 cut(s)	778, 1343, 1587
Eco147I	AGGCCT	1 cut(s)	1197
Eco24I	GRGCYC	5 cut(s)	144, 195, 395, 660, 987
Eco31I	GGTCTC	1 cut(s)	1355
Eco47I	GGWCC	2 cut(s)	204, 825
Eco53kI	GAGCTC	1 cut(s)	142
Eco57I	CTGAAG	3 cut(s)	398, 915, 920
Eco88I	CYCGRG	3 cut(s)	308, 449, 659
EcoICRI	GAGCTC	1 cut(s)	142
EcoO109I	RGGNCCY	3 cut(s)	204, 656, 825
EcoRII	CCWGG	2 cut(s)	144, 320
EcoT14I	CCWWGG	3 cut(s)	778, 1343, 1587
EcoT38I	GRGCYC	5 cut(s)	144, 195, 395, 660, 987
ErhI	CCWWGG	3 cut(s)	778, 1343, 1587
Esp3I	CGTCTC	1 cut(s)	450
FaeI	CATG	6 cut(s)	263, 543, 947, 1208, 1347, 1379
FalI	AAGNNNNNCTT	2 cut(s)	1055, 1087
FaqI	GGGAC	1 cut(s)	838
FatI	CATG	6 cut(s)	259, 539, 943, 1204, 1343, 1375
FauI	CCCGC	1 cut(s)	112
FauNDI	CATATG	1 cut(s)	817
FblI	GTMKAC	1 cut(s)	1335
Fnu4HI	GCNGC	8 cut(s)	108, 366, 425, 597, 684, 687, 690, 1599
FokI	GGATG	5 cut(s)	17, 689, 851, 974, 1440
FriOI	GRGCYC	5 cut(s)	144, 195, 395, 660, 987
Fsp4HI	GCNGC	8 cut(s)	108, 366, 425, 597, 684, 687, 690, 1599
FspBI	CTAG	2 cut(s)	1002, 1328
GlaI	GCGC	1 cut(s)	375
GluI	GCNGC	8 cut(s)	108, 366, 425, 597, 684, 687, 690, 1599
GsuI	CTGGAG	3 cut(s)	7, 269, 904
HaeIII	GGCC	5 cut(s)	193, 215, 407, 658, 1197
HapII	CCGG	1 cut(s)	558
HgaI	GACGC	3 cut(s)	429, 965, 1428
HhaI	GCGC	1 cut(s)	376
Hin1II	CATG	6 cut(s)	263, 543, 947, 1208, 1347, 1379
Hin6I	GCGC	1 cut(s)	374
HinP1I	GCGC	1 cut(s)	374
HincII	GTYRAC	1 cut(s)	883
HindII	GTYRAC	1 cut(s)	883
HindIII	AAGCTT	1 cut(s)	410
HinfI	GANTC	3 cut(s)	27, 1094, 1154
HpaI	GTTAAC	1 cut(s)	883
HpaII	CCGG	1 cut(s)	558
HphI	GGTGA	6 cut(s)	31, 37, 289, 572, 598, 1345
Hpy166II	GTNNAC	6 cut(s)	507, 563, 883, 916, 1336, 1497
Hpy188I	TCNGA	5 cut(s)	91, 211, 619, 1159, 1521
Hpy8I	GTNNAC	6 cut(s)	507, 563, 883, 916, 1336, 1497
Hpy99I	CGWCG	2 cut(s)	116, 445
HpyAV	CCTTC	4 cut(s)	373, 815, 998, 1543
HpyCH4III	ACNGT	8 cut(s)	188, 698, 1008, 1026, 1078, 1103, 1213, 1264
HpyCH4IV	ACGT	1 cut(s)	1554
HpyCH4V	TGCA	7 cut(s)	815, 850, 908, 953, 1350, 1445, 1465
HpyF10VI	GCNNNNNNNGC	6 cut(s)	365, 371, 437, 689, 698, 905
HpyF3I	CTNAG	6 cut(s)	339, 351, 360, 1062, 1192, 1392
HpySE526I	ACGT	1 cut(s)	1554
Hsp92II	CATG	6 cut(s)	263, 543, 947, 1208, 1347, 1379
HspAI	GCGC	1 cut(s)	374
KspAI	GTTAAC	1 cut(s)	883
Kzo9I	GATC	3 cut(s)	453, 1159, 1521
LguI	GCTCTTC	1 cut(s)	132
LmnI	GCTCC	4 cut(s)	147, 250, 322, 390
Lsp1109I	GCAGC	4 cut(s)	352, 608, 695, 701
LweI	GCATC	4 cut(s)	336, 406, 1359, 1426
MaeI	CTAG	2 cut(s)	1002, 1328
MaeII	ACGT	1 cut(s)	1554
MaeIII	GTNAC	5 cut(s)	535, 586, 1111, 1258, 1310
MalI	GATC	3 cut(s)	455, 1161, 1523
MbiI	CCGCTC	1 cut(s)	424
MboI	GATC	3 cut(s)	453, 1159, 1521
MflI	RGATCY	1 cut(s)	1159
MhlI	GDGCHC	6 cut(s)	144, 195, 395, 660, 666, 987
MlsI	TGGCCA	1 cut(s)	407
MluCI	AATT	7 cut(s)	383, 602, 646, 671, 783, 901, 1610
MluNI	TGGCCA	1 cut(s)	407
MmeI	TCCRAC	3 cut(s)	642, 1187, 1505
Mox20I	TGGCCA	1 cut(s)	407
MscI	TGGCCA	1 cut(s)	407
MseI	TTAA	6 cut(s)	255, 401, 882, 1073, 1304, 1401
MslI	CAYNNNNRTG	2 cut(s)	1317, 1345
Msp20I	TGGCCA	1 cut(s)	407
MspA1I	CMGCKG	2 cut(s)	686, 692
MspI	CCGG	1 cut(s)	558
MspR9I	CCNGG	2 cut(s)	146, 322
MvaI	CCWGG	2 cut(s)	146, 322
MvnI	CGCG	3 cut(s)	431, 440, 959
MwoI	GCNNNNNNNGC	6 cut(s)	365, 371, 437, 689, 698, 905
NcoI	CCATGG	1 cut(s)	1343
NdeI	CATATG	1 cut(s)	817
NdeII	GATC	3 cut(s)	453, 1159, 1521
NlaIII	CATG	6 cut(s)	263, 543, 947, 1208, 1347, 1379
NlaIV	GGNNCC	7 cut(s)	193, 392, 464, 493, 657, 658, 826
NmeAIII	GCCGAG	2 cut(s)	241, 445
NmuCI	GTSAC	4 cut(s)	535, 586, 1111, 1310
NspI	RCATGY	1 cut(s)	1379
OliI	CACNNNNGTG	1 cut(s)	1345
PaeR7I	CTCGAG	1 cut(s)	449
PagI	TCATGA	1 cut(s)	259
PceI	AGGCCT	1 cut(s)	1197
PciSI	GCTCTTC	1 cut(s)	132
PfeI	GAWTC	3 cut(s)	27, 1094, 1154
PflMI	CCANNNNNTGG	1 cut(s)	770
PinAI	ACCGGT	1 cut(s)	557
PkrI	GCNGC	8 cut(s)	109, 367, 426, 598, 685, 688, 691, 1600
PpuMI	RGGWCCY	2 cut(s)	204, 825
Psp124BI	GAGCTC	1 cut(s)	144
Psp5II	RGGWCCY	2 cut(s)	204, 825
Psp6I	CCWGG	2 cut(s)	144, 320
PspGI	CCWGG	2 cut(s)	144, 320
PspN4I	GGNNCC	7 cut(s)	193, 392, 464, 493, 657, 658, 826
PspOMI	GGGCCC	2 cut(s)	191, 656
PspPI	GGNCC	6 cut(s)	191, 192, 204, 656, 657, 825
PspPPI	RGGWCCY	2 cut(s)	204, 825
PstNI	CAGNNNCTG	1 cut(s)	856
PsuI	RGATCY	1 cut(s)	1159
PvuII	CAGCTG	1 cut(s)	692
RsaI	GTAC	6 cut(s)	314, 506, 917, 1210, 1326, 1496
RsaNI	GTAC	6 cut(s)	313, 505, 916, 1209, 1325, 1495
RseI	CAYNNNNRTG	2 cut(s)	1317, 1345
SacI	GAGCTC	1 cut(s)	144
SapI	GCTCTTC	1 cut(s)	132
SaqAI	TTAA	6 cut(s)	255, 401, 882, 1073, 1304, 1401
SatI	GCNGC	8 cut(s)	108, 366, 425, 597, 684, 687, 690, 1599
Sau3AI	GATC	3 cut(s)	453, 1159, 1521
Sau96I	GGNCC	6 cut(s)	191, 192, 204, 656, 657, 825
ScrFI	CCNGG	2 cut(s)	146, 322
SduI	GDGCHC	6 cut(s)	144, 195, 395, 660, 666, 987
SfaNI	GCATC	4 cut(s)	336, 406, 1359, 1426
Sfr274I	CTCGAG	1 cut(s)	449
SinI	GGWCC	2 cut(s)	204, 825
SlaI	CTCGAG	1 cut(s)	449
SmiMI	CAYNNNNRTG	2 cut(s)	1317, 1345
SmlI	CTYRAG	3 cut(s)	449, 608, 980
SmoI	CTYRAG	3 cut(s)	449, 608, 980
Sse9I	AATT	7 cut(s)	383, 602, 646, 671, 783, 901, 1610
SseBI	AGGCCT	1 cut(s)	1197
SsiI	CCGC	9 cut(s)	105, 108, 424, 438, 524, 686, 959, 1220, 1599
SspI	AATATT	1 cut(s)	846
SspMI	CTAG	2 cut(s)	1002, 1328
SstI	GAGCTC	1 cut(s)	144
StuI	AGGCCT	1 cut(s)	1197
StyD4I	CCNGG	2 cut(s)	144, 320
StyI	CCWWGG	3 cut(s)	778, 1343, 1587
TaaI	ACNGT	8 cut(s)	188, 698, 1008, 1026, 1078, 1103, 1213, 1264
TaiI	ACGT	1 cut(s)	1557
TaqI	TCGA	2 cut(s)	450, 1541
TasI	AATT	7 cut(s)	383, 602, 646, 671, 783, 901, 1610
TatI	WGTACW	2 cut(s)	915, 1494
TauI	GCSGC	4 cut(s)	110, 427, 689, 1601
TfiI	GAWTC	3 cut(s)	27, 1094, 1154
Tru1I	TTAA	6 cut(s)	255, 401, 882, 1073, 1304, 1401
Tru9I	TTAA	6 cut(s)	255, 401, 882, 1073, 1304, 1401
TscAI	CASTG	7 cut(s)	376, 514, 703, 817, 1108, 1120, 1372
TseFI	GTSAC	4 cut(s)	535, 586, 1111, 1310
TseI	GCWGC	4 cut(s)	365, 596, 683, 689
Tsp45I	GTSAC	4 cut(s)	535, 586, 1111, 1310
TspDTI	ATGAA	7 cut(s)	19, 834, 855, 932, 1018, 1065, 1464
TspRI	CASTG	7 cut(s)	376, 514, 703, 817, 1108, 1120, 1372
Van91I	CCANNNNNTGG	1 cut(s)	770
VpaK11BI	GGWCC	2 cut(s)	204, 825
XapI	RAATTY	4 cut(s)	602, 646, 671, 901
XceI	RCATGY	1 cut(s)	1379
XcmI	CCANNNNNNNNNTGG	1 cut(s)	506
XhoI	CTCGAG	1 cut(s)	449
XmiI	GTMKAC	1 cut(s)	1335
XspI	CTAG	2 cut(s)	1002, 1328

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.