Rh2CG388400

Protein disulfide isomerase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015135
Physical Location & Seq
Forward (+)
52948684 .. 52955129
6446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG388400.1

Sequence Viewer

Length: 1611 bp
ATGTTTAGACCAAAACCCACATCAAGATTCATCCTTTTAGCCCTCACCCTTCTCCTACTAGTCAATTTCCTAGCTTCAATCACAGCCTCTGAACCCACCACTTCTGAAAGTGAAGATAATGAATTGGAGGAGTTACTAGCATTAGATGATGAAGTGGAACAAGAAGAAGAAGAGCAAGGAGGGACTCCTAATGTGAGGTCTTCAGAGGCAGAGCTGTTAACCAAAGCCCAGAGGATTGTTCTTGAGCTTAACCATGACAATACCAAAAGGGTGATTGACAAGAATGAGTTTGTTCTGGTTCTTGGGTATGCTCCTTGGTGTGCCAGAAGTGCTGAGCTTATGCCTCAATTTGCTGAGGCTGCAACTTCACTCAGTGAATTGGGGAGTCCTCTTGTGATGGCTAAGCTTGATGCAGAGCGGTATCCCAAAACGGCGTCGCTGCTTGAGATCAAAGGGTTCCCTACTCTGCTTCTGTTTGTCAATGGCACCTCTCAAGTGTACACCGGTGGATTTTCTGCGGAAGAAATAGTGATATGGGCAAGGAAGAAGACTGGTGAGCCTGTTATTAGGATAAGCTCGGTGCCAGCGGCGGAACAATTTCTCAAGAAGCATCATATATTTGTTGTTGGTTTGTTTGAAAAATTTGAGGGGCCTTACTATAAAGAATTTGTAAAAGCAGCAACAGCTGACAATGCAATCCAGTTTGTAGAAGTAAGCGACATCAATGTTGCCAATGTTCTCTTTCCAAATATAAAACCAGCTAATCTTTTCCTTGGAATTGTGAAATCTGAGGCTGAAAGATACACTGCGTATGAAGGGACATTTGAGATGGAGCAAATATTGAAGTTCTTGGACTATAACAAGTTTCCATTAGTTAACAAACTGACTGAATCAAATTCTGCCACAGTTTACTCCAGCCCTCTTAAACTTCAGGTCATTGTCTTTGCAGAAGAAGATGAATTCAAGAAGCTTCTGGAGCCTCTTCAGGATATTGCTCGACAATTCAAGTCAGAGATAATGTTTATATATATAGACATCACAGATGAAAACCTTGCCAAGCCTTATTTAACGTTATTTGGGCTTGAAGAAGCAGAGAGCACTGTGGTGGCTGCTTTTGATGTCCGAGTCAACTCTAAATATCTATTAGAGTCAAATCCAACACCAAGCAATATAGAGGAGTTCTGCTCAGGGCTTCTGCATGGCACTTTGTCTCCATACTTCAAATCACAAGCAATACCTAATAACGCCAATGAAACCGTCCATGTTGCTGTTGGAAAGACATTTGATGACTTGGTTTTGAATAACCACAAGAATGTTCTACTAGAGGTGTTCACACCATGGTGCGTCACATGTGAAACCACTAGCAAGCGCGTCGAGAAGTTGGCTAAGCATTTCAAAAATTTGGATAGTTTATTCTTCGCAAAGATAGATGCTTCTGCAAATGAACATCCAAAACTGAAAGTAGATGACTATCCAACACTCTTATTTTATGCAGCCAATAATAAAGAGAACCCGATCAAGCTTTCTACGAAAACCAGCTTGAAGGACCTGGCCACATCAATCAACAAACATGTGAAAGCCAAAGATGAAGTTGCTAAAGATGAACTATAG

Protein Analysis

536

Amino Acids

60.15

Weight (kDa)

5.02

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 81 - 179 1.1e-14 Thioredoxin
Thioredoxin_6 PF13848 212 - 396 1.5e-16 Thioredoxin-like domain
Thioredoxin PF00085 421 - 521 9.4e-13 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16110
fragaria_vesca FvH4_6g31670
malus_domestica MD09G1209900.v1.1 MD17G1191800.v1.1
prunus_persica Prupe.3G067900_v2.0.a1
pyrus_communis pycom17g19880
rosa_chinensis RchiOBHm_Chr2g0138751
rosa_laevigata RLG00000019758
rosa_multiflora Rmu_sc0006243.1_g000002
rosa_roxburghii Rroxscaffold_2G00105970
rosa_rugosa Rorug02G0352600
rosa_samantha Rh2AG402200 Rh2BG405400 Rh2CG388400 Rh2DG422400
rosa_wichuraiana Rw2G032700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 485, 580
AccBSI CCGCTC 1 cut(s) 418
AccII CGCG 1 cut(s) 1371
AciI CCGC 4 cut(s) 418, 518, 587, 590
AclI AACGTT 1 cut(s) 1070
AcoI YGGCCR 1 cut(s) 1551
AcsI RAATTY 5 cut(s) 641, 665, 895, 959, 1399
AcuI CTGAAG 3 cut(s) 186, 914, 968
AcyI GRCGYC 1 cut(s) 434
AdeI CACNNNGTG 1 cut(s) 374
AfaI GTAC 1 cut(s) 500
AflIII ACRYGT 2 cut(s) 1349, 1570
AgeI ACCGGT 1 cut(s) 503
AhlI ACTAGT 1 cut(s) 58
AjnI CCWGG 1 cut(s) 1548
AleI CACNNNNGTG 2 cut(s) 1103, 1339
Alw21I GWGCWC 1 cut(s) 1100
Alw26I GTCTC 1 cut(s) 1215
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 2 cut(s) 650, 1551
ApeKI GCWGC 5 cut(s) 359, 439, 677, 1109, 1493
ApoI RAATTY 5 cut(s) 641, 665, 895, 959, 1399
AsiGI ACCGGT 1 cut(s) 503
Asp700I GAANNNNTTC 1 cut(s) 597
AspLEI GCGC 1 cut(s) 1371
AspS9I GGNCC 2 cut(s) 650, 1546
AsuHPI GGTGA 3 cut(s) 37, 283, 566
AvaII GGWCC 1 cut(s) 1546
BalI TGGCCA 1 cut(s) 1553
BanI GGYRCC 2 cut(s) 485, 580
BbsI GAAGAC 2 cut(s) 192, 554
Bbv12I GWGCWC 1 cut(s) 1100
BbvCI CCTCAGC 1 cut(s) 354
BbvI GCAGC 5 cut(s) 346, 426, 689, 1096, 1505
BccI CCATC 2 cut(s) 391, 823
BceAI ACGGC 1 cut(s) 447
BcgI CGANNNNNNTGC 2 cut(s) 1354, 1388
BciT130I CCWGG 1 cut(s) 1550
BciVI GTATCC 1 cut(s) 432
BcoDI GTCTC 1 cut(s) 1215
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 5 cut(s) 59, 71, 137, 1322, 1362
BfmI CTRYAG 1 cut(s) 1607
BfuI GTATCC 1 cut(s) 432
BisI GCNGC 6 cut(s) 360, 440, 588, 678, 1110, 1494
BlpI GCTNAGC 3 cut(s) 333, 402, 1386
BlsI GCNGC 6 cut(s) 361, 441, 589, 679, 1111, 1495
Bme1390I CCNGG 1 cut(s) 1550
Bme18I GGWCC 1 cut(s) 1546
BmgT120I GGNCC 2 cut(s) 650, 1546
BmiI GGNNCC 5 cut(s) 458, 487, 582, 651, 978
BmrFI CCNGG 1 cut(s) 1550
BmsI GCATC 3 cut(s) 400, 619, 1420
BpiI GAAGAC 2 cut(s) 192, 554
BplI GAGNNNNNCTC 2 cut(s) 1169, 1201
BpmI CTGGAG 2 cut(s) 898, 995
Bpu10I CCTNAGC 2 cut(s) 354, 1186
Bpu1102I GCTNAGC 3 cut(s) 333, 402, 1386
BpuEI CTTGAG 4 cut(s) 263, 464, 477, 587
BsaBI GATNNNNATC 1 cut(s) 1470
BsaHI GRCGYC 1 cut(s) 434
BsaJI CCNNGG 3 cut(s) 314, 772, 1337
BsaWI WCCGGW 1 cut(s) 503
BsaXI ACNNNNNCTCC 2 cut(s) 1195, 1225
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 2 cut(s) 556, 700
Bse8I GATNNNNATC 1 cut(s) 1470
BseBI CCWGG 1 cut(s) 1550
BseDI CCNNGG 3 cut(s) 314, 772, 1337
BseGI GGATG 2 cut(s) 30, 1447
BseJI GATNNNNATC 1 cut(s) 1470
BseMII CTCAG 5 cut(s) 324, 345, 385, 780, 1200
BseNI ACTGG 2 cut(s) 556, 700
BseRI GAGGAG 2 cut(s) 143, 1190
BseXI GCAGC 5 cut(s) 346, 426, 689, 1096, 1505
Bsh1236I CGCG 1 cut(s) 1371
BshFI GGCC 2 cut(s) 652, 1553
BshNI GGYRCC 2 cut(s) 485, 580
BshTI ACCGGT 1 cut(s) 503
BsiHKAI GWGCWC 1 cut(s) 1100
BsiSI CCGG 1 cut(s) 504
BslFI GGGAC 2 cut(s) 196, 832
BsmAI GTCTC 1 cut(s) 1215
BsmFI GGGAC 2 cut(s) 196, 832
BsnI GGCC 2 cut(s) 652, 1553
Bsp1286I GDGCHC 1 cut(s) 1100
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 2 cut(s) 447, 1515
Bsp1720I GCTNAGC 3 cut(s) 333, 402, 1386
Bsp19I CCATGG 1 cut(s) 1337
BspACI CCGC 4 cut(s) 418, 518, 587, 590
BspANI GGCC 2 cut(s) 652, 1553
BspCNI CTCAG 5 cut(s) 325, 346, 384, 781, 1199
BspFNI CGCG 1 cut(s) 1371
BspLI GGNNCC 5 cut(s) 458, 487, 582, 651, 978
BspQI GCTCTTC 1 cut(s) 165
BspT107I GGYRCC 2 cut(s) 485, 580
BsrBI CCGCTC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 503
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 2 cut(s) 556, 700
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 3 cut(s) 314, 772, 1337
BssMI GATC 2 cut(s) 447, 1515
BssNI GRCGYC 1 cut(s) 434
BssT1I CCWWGG 3 cut(s) 314, 772, 1337
Bst2UI CCWGG 1 cut(s) 1550
Bst4CI ACNGT 3 cut(s) 907, 1102, 1258
Bst6I CTCTTC 2 cut(s) 165, 987
BstACI GRCGYC 1 cut(s) 434
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 585, 1367
BstDEI CTNAG 7 cut(s) 333, 354, 371, 402, 789, 1186, 1386
BstDSI CCRYGG 1 cut(s) 1337
BstF5I GGATG 2 cut(s) 30, 1447
BstFNI CGCG 1 cut(s) 1371
BstHHI GCGC 1 cut(s) 1371
BstKTI GATC 2 cut(s) 450, 1518
BstMAI GTCTC 1 cut(s) 1215
BstMBI GATC 2 cut(s) 447, 1515
BstMWI GCNNNNNNNGC 5 cut(s) 329, 359, 683, 692, 976
BstNI CCWGG 1 cut(s) 1550
BstNSI RCATGY 2 cut(s) 1353, 1574
BstSCI CCNGG 1 cut(s) 1548
BstSFI CTRYAG 1 cut(s) 1607
BstUI CGCG 1 cut(s) 1371
BstV1I GCAGC 5 cut(s) 346, 426, 689, 1096, 1505
BstV2I GAAGAC 2 cut(s) 192, 554
BsuI GTATCC 1 cut(s) 432
BsuRI GGCC 2 cut(s) 652, 1553
BtgI CCRYGG 1 cut(s) 1337
BtsCI GGATG 2 cut(s) 30, 1447
BtsI GCAGTG 1 cut(s) 804
BtsIMutI CAGTG 3 cut(s) 379, 804, 1098
Cac8I GCNNGC 2 cut(s) 585, 1367
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 1 cut(s) 1371
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 2 cut(s) 650, 1546
CseI GACGC 3 cut(s) 423, 1333, 1360
Csp6I GTAC 1 cut(s) 499
CspAI ACCGGT 1 cut(s) 503
CviAII CATG 6 cut(s) 254, 1199, 1262, 1338, 1350, 1571
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 7 cut(s) 333, 354, 371, 402, 789, 1186, 1386
DpnI GATC 2 cut(s) 449, 1517
DpnII GATC 2 cut(s) 447, 1515
DraIII CACNNNGTG 1 cut(s) 374
EaeI YGGCCR 1 cut(s) 1551
Eam1104I CTCTTC 2 cut(s) 165, 987
EarI CTCTTC 2 cut(s) 165, 987
EciI GGCGGA 1 cut(s) 605
Eco130I CCWWGG 3 cut(s) 314, 772, 1337
Eco47I GGWCC 1 cut(s) 1546
Eco57I CTGAAG 3 cut(s) 186, 914, 968
EcoO109I RGGNCCY 2 cut(s) 650, 1546
EcoRI GAATTC 1 cut(s) 959
EcoRII CCWGG 1 cut(s) 1548
EcoT14I CCWWGG 3 cut(s) 314, 772, 1337
ErhI CCWWGG 3 cut(s) 314, 772, 1337
FaeI CATG 6 cut(s) 257, 1202, 1265, 1341, 1353, 1574
FaqI GGGAC 2 cut(s) 196, 832
FatI CATG 6 cut(s) 253, 1198, 1261, 1337, 1349, 1570
Fnu4HI GCNGC 6 cut(s) 360, 440, 588, 678, 1110, 1494
FokI GGATG 2 cut(s) 17, 1434
Fsp4HI GCNGC 6 cut(s) 360, 440, 588, 678, 1110, 1494
FspBI CTAG 5 cut(s) 59, 71, 137, 1322, 1362
GlaI GCGC 1 cut(s) 1370
GluI GCNGC 6 cut(s) 360, 440, 588, 678, 1110, 1494
GsuI CTGGAG 2 cut(s) 898, 995
HaeIII GGCC 2 cut(s) 652, 1553
HapII CCGG 1 cut(s) 504
HgaI GACGC 3 cut(s) 423, 1333, 1360
HhaI GCGC 1 cut(s) 1371
Hin1I GRCGYC 1 cut(s) 434
Hin1II CATG 6 cut(s) 257, 1202, 1265, 1341, 1353, 1574
Hin6I GCGC 1 cut(s) 1369
HinP1I GCGC 1 cut(s) 1369
HincII GTYRAC 3 cut(s) 219, 877, 1129
HindII GTYRAC 3 cut(s) 219, 877, 1129
HindIII AAGCTT 3 cut(s) 404, 968, 1520
HinfI GANTC 6 cut(s) 27, 184, 385, 890, 1125, 1148
HpaI GTTAAC 2 cut(s) 219, 877
HpaII CCGG 1 cut(s) 504
HphI GGTGA 3 cut(s) 37, 283, 566
Hpy166II GTNNAC 7 cut(s) 219, 499, 501, 877, 910, 1129, 1332
Hpy188I TCNGA 6 cut(s) 91, 106, 205, 790, 1012, 1124
Hpy188III TCNNGA 7 cut(s) 24, 242, 604, 964, 974, 986, 1375
Hpy8I GTNNAC 7 cut(s) 219, 499, 501, 877, 910, 1129, 1332
Hpy99I CGWCG 2 cut(s) 439, 1376
HpyAV CCTTC 3 cut(s) 59, 809, 1537
HpyCH4III ACNGT 3 cut(s) 907, 1102, 1258
HpyCH4IV ACGT 1 cut(s) 1070
HpyCH4V TGCA 7 cut(s) 362, 413, 695, 947, 1198, 1439, 1493
HpyF10VI GCNNNNNNNGC 5 cut(s) 329, 359, 683, 692, 976
HpyF3I CTNAG 7 cut(s) 333, 354, 371, 402, 789, 1186, 1386
HpySE526I ACGT 1 cut(s) 1070
Hsp92I GRCGYC 1 cut(s) 434
Hsp92II CATG 6 cut(s) 257, 1202, 1265, 1341, 1353, 1574
HspAI GCGC 1 cut(s) 1369
KspAI GTTAAC 2 cut(s) 219, 877
Kzo9I GATC 2 cut(s) 447, 1515
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 3 cut(s) 316, 832, 976
Lsp1109I GCAGC 5 cut(s) 346, 426, 689, 1096, 1505
LweI GCATC 3 cut(s) 400, 619, 1420
MaeI CTAG 5 cut(s) 59, 71, 137, 1322, 1362
MaeII ACGT 1 cut(s) 1070
MaeIII GTNAC 2 cut(s) 132, 1345
MalI GATC 2 cut(s) 449, 1517
MbiI CCGCTC 1 cut(s) 418
MboI GATC 2 cut(s) 447, 1515
MhlI GDGCHC 1 cut(s) 1100
MlsI TGGCCA 1 cut(s) 1553
MluNI TGGCCA 1 cut(s) 1553
MlyI GAGTC 4 cut(s) 178, 394, 1134, 1157
MmeI TCCRAC 3 cut(s) 1181, 1252, 1499
Mox20I TGGCCA 1 cut(s) 1553
MroXI GAANNNNTTC 1 cut(s) 597
MscI TGGCCA 1 cut(s) 1553
MseI TTAA 5 cut(s) 218, 249, 876, 924, 1067
MslI CAYNNNNRTG 3 cut(s) 1103, 1311, 1339
Msp20I TGGCCA 1 cut(s) 1553
MspA1I CMGCKG 2 cut(s) 587, 686
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 1 cut(s) 1550
MvaI CCWGG 1 cut(s) 1550
MvnI CGCG 1 cut(s) 1371
MwoI GCNNNNNNNGC 5 cut(s) 329, 359, 683, 692, 976
NcoI CCATGG 1 cut(s) 1337
NdeII GATC 2 cut(s) 447, 1515
NlaIII CATG 6 cut(s) 257, 1202, 1265, 1341, 1353, 1574
NlaIV GGNNCC 5 cut(s) 458, 487, 582, 651, 978
NmuCI GTSAC 1 cut(s) 1345
NspI RCATGY 2 cut(s) 1353, 1574
OliI CACNNNNGTG 2 cut(s) 1103, 1339
PciI ACATGT 2 cut(s) 1349, 1570
PciSI GCTCTTC 1 cut(s) 165
PdmI GAANNNNTTC 1 cut(s) 597
PfeI GAWTC 2 cut(s) 27, 890
PinAI ACCGGT 1 cut(s) 503
PkrI GCNGC 6 cut(s) 361, 441, 589, 679, 1111, 1495
PleI GAGTC 4 cut(s) 178, 393, 1133, 1156
PpsI GAGTC 4 cut(s) 178, 393, 1133, 1156
PpuMI RGGWCCY 1 cut(s) 1546
PscI ACATGT 2 cut(s) 1349, 1570
Psp1406I AACGTT 1 cut(s) 1070
Psp5II RGGWCCY 1 cut(s) 1546
Psp6I CCWGG 1 cut(s) 1548
PspGI CCWGG 1 cut(s) 1548
PspN4I GGNNCC 5 cut(s) 458, 487, 582, 651, 978
PspPI GGNCC 2 cut(s) 650, 1546
PspPPI RGGWCCY 1 cut(s) 1546
PstNI CAGNNNCTG 1 cut(s) 89
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
RseI CAYNNNNRTG 3 cut(s) 1103, 1311, 1339
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 5 cut(s) 218, 249, 876, 924, 1067
SatI GCNGC 6 cut(s) 360, 440, 588, 678, 1110, 1494
Sau3AI GATC 2 cut(s) 447, 1515
Sau96I GGNCC 2 cut(s) 650, 1546
SchI GAGTC 4 cut(s) 178, 394, 1134, 1157
ScrFI CCNGG 1 cut(s) 1550
SduI GDGCHC 1 cut(s) 1100
SfaNI GCATC 3 cut(s) 400, 619, 1420
SfcI CTRYAG 1 cut(s) 1607
SgrAI CRCCGGYG 1 cut(s) 503
SinI GGWCC 1 cut(s) 1546
SmiMI CAYNNNNRTG 3 cut(s) 1103, 1311, 1339
SmlI CTYRAG 4 cut(s) 242, 443, 492, 602
SmoI CTYRAG 4 cut(s) 242, 443, 492, 602
SpeI ACTAGT 1 cut(s) 58
SsiI CCGC 4 cut(s) 418, 518, 587, 590
SspI AATATT 1 cut(s) 840
SspMI CTAG 5 cut(s) 59, 71, 137, 1322, 1362
StyD4I CCNGG 1 cut(s) 1548
StyI CCWWGG 3 cut(s) 314, 772, 1337
TaaI ACNGT 3 cut(s) 907, 1102, 1258
TaiI ACGT 1 cut(s) 1073
TaqI TCGA 2 cut(s) 997, 1374
TatI WGTACW 1 cut(s) 498
TauI GCSGC 1 cut(s) 590
TfiI GAWTC 2 cut(s) 27, 890
Tru1I TTAA 5 cut(s) 218, 249, 876, 924, 1067
Tru9I TTAA 5 cut(s) 218, 249, 876, 924, 1067
TscAI CASTG 3 cut(s) 379, 811, 1105
TseFI GTSAC 1 cut(s) 1345
TseI GCWGC 5 cut(s) 359, 439, 677, 1109, 1493
Tsp45I GTSAC 1 cut(s) 1345
TspDTI ATGAA 9 cut(s) 19, 135, 165, 828, 972, 1059, 1266, 1458, 1602
TspRI CASTG 3 cut(s) 379, 811, 1105
VpaK11BI GGWCC 1 cut(s) 1546
XapI RAATTY 5 cut(s) 641, 665, 895, 959, 1399
XceI RCATGY 2 cut(s) 1353, 1574
XcmI CCANNNNNNNNNTGG 1 cut(s) 1268
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 5 cut(s) 59, 71, 137, 1322, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.