Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0014988

Physical Location & Seq

Genomic Coordinates Reverse (-)

31564766 .. 31566877

Gene Structure 2112 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1246400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2112 bp

ATGTCTCCTGCTCCGACGATCCTCTCTGTCTTCCTGGTCATCACTTGGACCATTTTTCTGATCACAACATTCCCAACTTTCGGCGGCGCCACAGAGTTCGTCTTCAACACCAACTTCAACTCCAGCAGCAACCTCAGCCTCTACGGCAGCGCCACCATCCAATCCTCCATCCTCAGCCTCACCAATGACTCCACCACCTTCTCACTGGGACGCGCTTTCTACTCTCACAAAATCCCCACCAAATACGCCGACTCTTCAGAAGCCCTTAATTTCTCCACCTCTTTTATTTTCTCCCTTGCTCCCGTCAAAAACCTCCTTCCCGGCCACGGTTTTGCCTTCCTCTTCTCACCCTTCTCTGGCTTAAGCGGTGCAAGCTCCTCGCAGCACTTGGGTTTTTTCAACTTCTCCAACAACGGCGACCCCAACAACAACATCTTCGGTGTCGAGTTTGATGTCTTCGAAAACCAAGAGTTCCAGGATCCTGATGACAACCATGTTGGTTTGGACATCAACTCCCTTACGTCTAATGCTTCTAGCACCGCAGGGTATTGGAGTGGCGGACTTGATCAAGATTTCAACGAGTTGAAGCTCAACAATGGAATTAACTATCAAGTGTGGATTGATTTTATGGGCTCGAGAATTAACGTGACCATGGCTCGTGCCGGCATGGCAAAGCCGCGGAGGCCGCTGATAAGTGAGGTGGTGGATCTGTCCCGGGTGCTTTTGGATGAAATGTACGTGGGGTTTTGCGCGGCAACTGGAGCATTGGTTGAGAGCCATAGGATATTGGCTTGGAGCTTTAGCAATTCTAATTTCTCCATCAGTGACGCTTTGGTCACTCAGAATTTGCCTTCCTTTGTGGTGTCAGAGAACAAGTCTGTTTTCGGAGTAAAAGGGTTTGTTGTGGGAGTTAGTGTTGGCGGGTTTTTGGTTGCTTGGTGCGTGGTTTTGGTGTGTTTCATTTTGGTGAGGAGGAAGAGGAGAAAGCGAATGAAAGGAGAGGAGGATGTAGAGAAAGTTGAAGATTGGGAATTGGAGTACTGGCCTCACCGGATTGATTACGAAGAGATACATACCGCGACAGAGGGATTTTCGGAGAAGAAGGTGATTGGGATTGGAGGGCTTGGGAAGGTGTATAAAGGGGTACTAGGAGGAGCAGAAGTAGCAGTGAAAAGAATTCTGCTTCAAAGTGAACGTGGGTTGAAAGAGTTCGTGGCTGAGGTTTCGAGCTTGGGAAGATTGAAGCATAGGAATTTGGTAGGACTGAGAGGTTGGTGCAAGAGAGAAAAGGGCAGTCTAATCTTGGTTTATGATTACATGGAAAATGGTAGCTTGGATAAGAGGATATTTGAGTGCAATGAGAGAAACTTGTTGAGTTGGGAAGAGAGAATTAAGGTGTTGAAAGATGTTGCTAGTGGGATTTTGTATTTGCACGAGGGTTGGGAAGCTAAGGTTTTGCATAGAGATATCAAATCAAGCAATGTGCTACTTGATAAGGATATGAATGCTAGGTTAGGAGATTTCGGATTGGCCCGAATGCACCTACACGGGGAAATGACGGGCACAACACAAGTGGTTGGGACGGCAGGGTACATGGCACCCGAAGTGGTTCGAACTGGAAGAGCCTCAGCTGAGGTTGATGTGTTTGGTTTTGGGATATTAGTACTAGAGGTGGTGTGTGGGCGGAGACCGATAGAGGATGGGAAGCTGGGGTTGGTTGATTGGTGTTGGAGGCTAATGGAGAAGGGAAGGTTGAACGTAGCACTTGATGATCGGCTCAAGGCAAAGGGCGGATACAACGTGGAGGACGTCGAGAGATTGCTTCATTTGGGATTGTTGTGTGCATATCCTGAAGCTCATGAGAGGCCAACAATGAGGCAAGTTATGAAGGTGCTTGAAGGAGCAAGTGACGATGATGGCATTGATGGCAATCTGTCCCAAGAGGAAGGAATGCATGTAAATTTGCTGCACAAGATTAGAACAACTCCGATGTGGTCGACTTATCGGTGGAACATGAGAGGGCAGGGACACCCTACATTTGAAGACATGAAAAATTCTTTATCTTCTACATCATCTTTGTCTGGATCAGATATCATTAGAGTTGGTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Lectin_legB Pkinase

Protein Analysis

704

Amino Acids

78.18

Weight (kDa)

6.06

Isoelectric Point (pI)

35.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	31 - 272	3.2e-65	Legume lectin domain
Pkinase	PF00069	364 - 569	7.8e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	367 - 631	3.3e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014988)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28350
fragaria_vesca	FvH4_6g26300
malus_domestica	MD09G1246400.v1.1 MD17G1239200.v1.1 MD17G1239300.v1.1
prunus_persica	Prupe.3G131900_v2.0.a1
pyrus_communis	pycom09g16380
rosa_chinensis	RchiOBHm_Chr2g0126741
rosa_laevigata	RLG00000018918
rosa_roxburghii	Rroxscaffold_2G00117000
rosa_rugosa	Rorug02G0264500
rosa_samantha	Rh2AG321600 Rh2BG329700 Rh2CG309400 Rh2DG349000
rosa_wichuraiana	Rw2G026060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	833
AatII	GACGTC	1 cut(s)	1812
AccB1I	GGYRCC	2 cut(s)	86, 1597
AccI	GTMKAC	1 cut(s)	1997
AccII	CGCG	4 cut(s)	213, 679, 752, 1079
AclWI	GGATC	5 cut(s)	13, 473, 486, 714, 2092
AcoI	YGGCCR	1 cut(s)	322
AcsI	RAATTY	5 cut(s)	844, 1176, 1252, 1960, 2053
AcuI	CTGAAG	2 cut(s)	240, 1872
AcyI	GRCGYC	2 cut(s)	87, 1809
AfaI	GTAC	5 cut(s)	737, 1040, 1146, 1592, 1665
AfiI	CCNNNNNNNGG	4 cut(s)	80, 326, 356, 1549
AflII	CTTAAG	1 cut(s)	361
AjnI	CCWGG	2 cut(s)	33, 474
AjuI	GAANNNNNNNTTGG	2 cut(s)	1697, 1729
AluBI	AGCT	9 cut(s)	375, 589, 798, 1230, 1332, 1448, 1631, 1708, 1856
AluI	AGCT	9 cut(s)	375, 589, 798, 1230, 1332, 1448, 1631, 1708, 1856
Alw26I	GTCTC	2 cut(s)	9, 1681
AlwI	GGATC	5 cut(s)	13, 473, 486, 714, 2092
Ama87I	CYCGRG	2 cut(s)	634, 714
AoxI	GGCC	5 cut(s)	322, 683, 1043, 1530, 1865
ApeKI	GCWGC	4 cut(s)	126, 147, 382, 1966
ApoI	RAATTY	5 cut(s)	844, 1176, 1252, 1960, 2053
Asp700I	GAANNNNTTC	2 cut(s)	1208, 1608
AspLEI	GCGC	4 cut(s)	89, 152, 215, 752
AspS9I	GGNCC	2 cut(s)	48, 1531
AsuC2I	CCSGG	3 cut(s)	321, 715, 716
AsuHPI	GGTGA	5 cut(s)	172, 339, 979, 1040, 1117
AsuII	TTCGAA	2 cut(s)	459, 1612
AvaI	CYCGRG	2 cut(s)	634, 714
AvaII	GGWCC	1 cut(s)	48
BaeGI	GKGCMC	1 cut(s)	1565
BamHI	GGATCC	1 cut(s)	478
BanI	GGYRCC	2 cut(s)	86, 1597
BanII	GRGCYC	1 cut(s)	635
BauI	CACGAG	2 cut(s)	657, 1433
BbsI	GAAGAC	4 cut(s)	22, 94, 448, 2049
BbvCI	CCTCAGC	5 cut(s)	134, 173, 1218, 1627, 1632
BbvI	GCAGC	4 cut(s)	138, 159, 394, 1953
BccI	CCATC	6 cut(s)	164, 176, 827, 1694, 1910, 1919
BceAI	ACGGC	3 cut(s)	160, 430, 1599
BcgI	CGANNNNNNTGC	2 cut(s)	360, 394
BciT130I	CCWGG	2 cut(s)	35, 476
BciVI	GTATCC	1 cut(s)	1787
BclI	TGATCA	2 cut(s)	60, 565
BcnI	CCSGG	3 cut(s)	321, 715, 716
BcoDI	GTCTC	2 cut(s)	9, 1681
BfaI	CTAG	5 cut(s)	534, 1148, 1413, 1509, 1667
BfoI	RGCGCY	2 cut(s)	90, 153
BfrI	CTTAAG	1 cut(s)	361
BfuI	GTATCC	1 cut(s)	1787
BglI	GCCNNNNNGGC	3 cut(s)	144, 668, 682
BisI	GCNGC	8 cut(s)	85, 127, 148, 383, 677, 686, 753, 1967
BlsI	GCNGC	8 cut(s)	86, 128, 149, 384, 678, 687, 754, 1968
BmcAI	AGTACT	2 cut(s)	1040, 1665
Bme1390I	CCNGG	5 cut(s)	35, 321, 476, 715, 716
Bme18I	GGWCC	1 cut(s)	48
BmeT110I	CYCGRG	2 cut(s)	634, 714
BmgT120I	GGNCC	2 cut(s)	48, 1531
BmiI	GGNNCC	3 cut(s)	88, 480, 1599
BmrFI	CCNGG	5 cut(s)	35, 321, 476, 715, 716
BmrI	ACTGGG	1 cut(s)	215
BmuI	ACTGGG	1 cut(s)	215
BpiI	GAAGAC	4 cut(s)	22, 94, 448, 2049
BpmI	CTGGAG	2 cut(s)	106, 780
Bpu10I	CCTNAGC	6 cut(s)	134, 173, 1218, 1449, 1627, 1632
Bpu14I	TTCGAA	2 cut(s)	459, 1612
BpuEI	CTTGAG	1 cut(s)	1763
BpuMI	CCSGG	3 cut(s)	321, 715, 716
BsaAI	YACGTR	1 cut(s)	739
BsaBI	GATNNNNATC	1 cut(s)	1929
BsaHI	GRCGYC	2 cut(s)	87, 1809
BsaI	GGTCTC	1 cut(s)	1681
BsaJI	CCNNGG	4 cut(s)	325, 651, 677, 714
BsaWI	WCCGGW	1 cut(s)	1050
Bsc4I	CCNNNNNNNGG	4 cut(s)	80, 326, 356, 1549
Bse118I	RCCGGY	1 cut(s)	662
Bse1I	ACTGG	4 cut(s)	210, 763, 1046, 1621
Bse3DI	GCAATG	2 cut(s)	1363, 1486
Bse8I	GATNNNNATC	1 cut(s)	1929
BseBI	CCWGG	2 cut(s)	35, 476
BseDI	CCNNGG	4 cut(s)	325, 651, 677, 714
BseGI	GGATG	5 cut(s)	156, 168, 733, 1012, 1705
BseJI	GATNNNNATC	1 cut(s)	1929
BseLI	CCNNNNNNNGG	4 cut(s)	80, 326, 356, 1549
BseMI	GCAATG	2 cut(s)	1363, 1486
BseMII	CTCAG	7 cut(s)	148, 187, 854, 1209, 1256, 1623, 1641
BseNI	ACTGG	4 cut(s)	210, 763, 1046, 1621
BseRI	GAGGAG	5 cut(s)	367, 985, 994, 1016, 1167
BseSI	GKGCMC	1 cut(s)	1565
BseXI	GCAGC	4 cut(s)	138, 159, 394, 1953
BseYI	CCCAGC	1 cut(s)	1708
BsgI	GTGCAG	1 cut(s)	1952
Bsh1236I	CGCG	4 cut(s)	213, 679, 752, 1079
BshFI	GGCC	5 cut(s)	324, 685, 1045, 1532, 1867
BshNI	GGYRCC	2 cut(s)	86, 1597
BsiHKCI	CYCGRG	2 cut(s)	634, 714
BsiSI	CCGG	4 cut(s)	321, 663, 715, 1051
BslFI	GGGAC	5 cut(s)	222, 697, 1594, 1921, 2040
BslI	CCNNNNNNNGG	4 cut(s)	80, 326, 356, 1549
BsmAI	GTCTC	2 cut(s)	9, 1681
BsmFI	GGGAC	5 cut(s)	222, 697, 1594, 1921, 2040
BsmI	GAATGC	3 cut(s)	1510, 1542, 1956
BsnI	GGCC	5 cut(s)	324, 685, 1045, 1532, 1867
Bso31I	GGTCTC	1 cut(s)	1681
BsoBI	CYCGRG	2 cut(s)	634, 714
Bsp119I	TTCGAA	2 cut(s)	459, 1612
Bsp1286I	GDGCHC	2 cut(s)	635, 1565
Bsp143I	GATC	7 cut(s)	18, 60, 478, 565, 706, 1771, 2084
Bsp19I	CCATGG	1 cut(s)	651
BspANI	GGCC	5 cut(s)	324, 685, 1045, 1532, 1867
BspCNI	CTCAG	7 cut(s)	147, 186, 853, 1210, 1257, 1624, 1640
BspFNI	CGCG	4 cut(s)	213, 679, 752, 1079
BspHI	TCATGA	1 cut(s)	1858
BspLI	GGNNCC	3 cut(s)	88, 480, 1599
BspPI	GGATC	5 cut(s)	13, 473, 486, 714, 2092
BspQI	GCTCTTC	1 cut(s)	1615
BspT104I	TTCGAA	2 cut(s)	459, 1612
BspT107I	GGYRCC	2 cut(s)	86, 1597
BspTI	CTTAAG	1 cut(s)	361
BspTNI	GGTCTC	1 cut(s)	1681
BsrDI	GCAATG	2 cut(s)	1363, 1486
BsrFI	RCCGGY	1 cut(s)	662
BsrI	ACTGG	4 cut(s)	210, 763, 1046, 1621
BssAI	RCCGGY	1 cut(s)	662
BssECI	CCNNGG	4 cut(s)	325, 651, 677, 714
BssMI	GATC	7 cut(s)	18, 60, 478, 565, 706, 1771, 2084
BssNI	GRCGYC	2 cut(s)	87, 1809
BssSI	CACGAG	2 cut(s)	657, 1433
BssT1I	CCWWGG	1 cut(s)	651
Bst2BI	CACGAG	2 cut(s)	657, 1433
Bst2UI	CCWGG	2 cut(s)	35, 476
Bst4CI	ACNGT	1 cut(s)	329
Bst6I	CTCTTC	6 cut(s)	259, 347, 971, 1059, 1377, 1615
BstACI	GRCGYC	2 cut(s)	87, 1809
BstAFI	CTTAAG	1 cut(s)	361
BstBAI	YACGTR	1 cut(s)	739
BstBI	TTCGAA	2 cut(s)	459, 1612
BstC8I	GCNNGC	2 cut(s)	373, 664
BstDEI	CTNAG	8 cut(s)	134, 173, 840, 1218, 1265, 1449, 1627, 1632
BstDSI	CCRYGG	3 cut(s)	325, 651, 677
BstF5I	GGATG	5 cut(s)	156, 168, 733, 1012, 1705
BstFNI	CGCG	4 cut(s)	213, 679, 752, 1079
BstH2I	RGCGCY	2 cut(s)	90, 153
BstHHI	GCGC	4 cut(s)	89, 152, 215, 752
BstKTI	GATC	7 cut(s)	21, 63, 481, 568, 709, 1774, 2087
BstMAI	GTCTC	2 cut(s)	9, 1681
BstMBI	GATC	7 cut(s)	18, 60, 478, 565, 706, 1771, 2084
BstMWI	GCNNNNNNNGC	9 cut(s)	135, 144, 372, 668, 682, 685, 761, 1163, 1926
BstNI	CCWGG	2 cut(s)	35, 476
BstNSI	RCATGY	1 cut(s)	1958
BstSCI	CCNGG	5 cut(s)	33, 319, 474, 713, 714
BstSLI	GKGCMC	1 cut(s)	1565
BstUI	CGCG	4 cut(s)	213, 679, 752, 1079
BstV1I	GCAGC	4 cut(s)	138, 159, 394, 1953
BstV2I	GAAGAC	4 cut(s)	22, 94, 448, 2049
BstX2I	RGATCY	2 cut(s)	478, 706
BstYI	RGATCY	2 cut(s)	478, 706
BsuI	GTATCC	1 cut(s)	1787
BsuRI	GGCC	5 cut(s)	324, 685, 1045, 1532, 1867
BtgI	CCRYGG	3 cut(s)	325, 651, 677
BtsCI	GGATG	5 cut(s)	156, 168, 733, 1012, 1705
BtsI	GCAGTG	1 cut(s)	1173
BtsIMutI	CAGTG	3 cut(s)	203, 829, 1173
Cac8I	GCNNGC	2 cut(s)	373, 664
CciI	TCATGA	1 cut(s)	1858
CfoI	GCGC	4 cut(s)	89, 152, 215, 752
Cfr10I	RCCGGY	1 cut(s)	662
Cfr13I	GGNCC	2 cut(s)	48, 1531
Cfr42I	CCGCGG	1 cut(s)	680
Cfr9I	CCCGGG	1 cut(s)	714
CseI	GACGC	2 cut(s)	219, 836
Csp6I	GTAC	5 cut(s)	736, 1039, 1145, 1591, 1664
CspCI	CAANNNNNGTGG	2 cut(s)	1554, 1589
CviAII	CATG	9 cut(s)	494, 652, 667, 1318, 1594, 1859, 1955, 2014, 2047
CviQI	GTAC	5 cut(s)	736, 1039, 1145, 1591, 1664
DdeI	CTNAG	8 cut(s)	134, 173, 840, 1218, 1265, 1449, 1627, 1632
DinI	GGCGCC	1 cut(s)	88
DpnI	GATC	7 cut(s)	20, 62, 480, 567, 708, 1773, 2086
DpnII	GATC	7 cut(s)	18, 60, 478, 565, 706, 1771, 2084
DrdI	GACNNNNNNGTC	1 cut(s)	833
DseDI	GACNNNNNNGTC	1 cut(s)	833
EaeI	YGGCCR	1 cut(s)	322
Eam1104I	CTCTTC	6 cut(s)	259, 347, 971, 1059, 1377, 1615
EarI	CTCTTC	6 cut(s)	259, 347, 971, 1059, 1377, 1615
EciI	GGCGGA	3 cut(s)	573, 1699, 1806
Eco130I	CCWWGG	1 cut(s)	651
Eco24I	GRGCYC	1 cut(s)	635
Eco31I	GGTCTC	1 cut(s)	1681
Eco32I	GATATC	2 cut(s)	1468, 2092
Eco47I	GGWCC	1 cut(s)	48
Eco57I	CTGAAG	2 cut(s)	240, 1872
Eco88I	CYCGRG	2 cut(s)	634, 714
EcoRI	GAATTC	1 cut(s)	1176
EcoRII	CCWGG	2 cut(s)	33, 474
EcoRV	GATATC	2 cut(s)	1468, 2092
EcoT14I	CCWWGG	1 cut(s)	651
EcoT22I	ATGCAT	1 cut(s)	1956
EcoT38I	GRGCYC	1 cut(s)	635
EgeI	GGCGCC	1 cut(s)	88
EheI	GGCGCC	1 cut(s)	88
ErhI	CCWWGG	1 cut(s)	651
FaeI	CATG	9 cut(s)	497, 655, 670, 1321, 1597, 1862, 1958, 2017, 2050
FaqI	GGGAC	5 cut(s)	222, 697, 1594, 1921, 2040
FatI	CATG	9 cut(s)	493, 651, 666, 1317, 1593, 1858, 1954, 2013, 2046
FauI	CCCGC	1 cut(s)	914
FbaI	TGATCA	2 cut(s)	60, 565
FblI	GTMKAC	1 cut(s)	1997
Fnu4HI	GCNGC	8 cut(s)	85, 127, 148, 383, 677, 686, 753, 1967
FokI	GGATG	5 cut(s)	143, 155, 740, 1019, 1712
FriOI	GRGCYC	1 cut(s)	635
Fsp4HI	GCNGC	8 cut(s)	85, 127, 148, 383, 677, 686, 753, 1967
FspBI	CTAG	5 cut(s)	534, 1148, 1413, 1509, 1667
GlaI	GCGC	4 cut(s)	88, 151, 214, 751
GluI	GCNGC	8 cut(s)	85, 127, 148, 383, 677, 686, 753, 1967
GsaI	CCCAGC	1 cut(s)	1712
GsuI	CTGGAG	2 cut(s)	106, 780
HaeII	RGCGCY	2 cut(s)	90, 153
HaeIII	GGCC	5 cut(s)	324, 685, 1045, 1532, 1867
HapII	CCGG	4 cut(s)	321, 663, 715, 1051
HgaI	GACGC	2 cut(s)	219, 836
HhaI	GCGC	4 cut(s)	89, 152, 215, 752
Hin1I	GRCGYC	2 cut(s)	87, 1809
Hin1II	CATG	9 cut(s)	497, 655, 670, 1321, 1597, 1862, 1958, 2017, 2050
Hin6I	GCGC	4 cut(s)	87, 150, 213, 750
HinP1I	GCGC	4 cut(s)	87, 150, 213, 750
HincII	GTYRAC	1 cut(s)	1998
HindII	GTYRAC	1 cut(s)	1998
HinfI	GANTC	2 cut(s)	188, 251
HpaII	CCGG	4 cut(s)	321, 663, 715, 1051
HphI	GGTGA	5 cut(s)	172, 339, 979, 1040, 1117
Hpy166II	GTNNAC	2 cut(s)	1193, 1998
Hpy188III	TCNNGA	7 cut(s)	482, 569, 636, 1813, 1850, 1859, 2082
Hpy8I	GTNNAC	2 cut(s)	1193, 1998
Hpy99I	CGWCG	2 cut(s)	19, 1814
HpyCH4III	ACNGT	1 cut(s)	329
HpyCH4IV	ACGT	7 cut(s)	521, 645, 738, 1195, 1758, 1800, 1809
HpyCH4V	TGCA	9 cut(s)	371, 1278, 1356, 1432, 1459, 1540, 1844, 1954, 1969
HpyF10VI	GCNNNNNNNGC	9 cut(s)	135, 144, 372, 668, 682, 685, 761, 1163, 1926
HpyF3I	CTNAG	8 cut(s)	134, 173, 840, 1218, 1265, 1449, 1627, 1632
HpySE526I	ACGT	7 cut(s)	521, 645, 738, 1195, 1758, 1800, 1809
Hsp92I	GRCGYC	2 cut(s)	87, 1809
Hsp92II	CATG	9 cut(s)	497, 655, 670, 1321, 1597, 1862, 1958, 2017, 2050
HspAI	GCGC	4 cut(s)	87, 150, 213, 750
KasI	GGCGCC	1 cut(s)	86
KroI	GCCGGC	1 cut(s)	662
KroNI	GCCGGC	1 cut(s)	664
Ksp22I	TGATCA	2 cut(s)	60, 565
KspI	CCGCGG	1 cut(s)	680
Kzo9I	GATC	7 cut(s)	18, 60, 478, 565, 706, 1771, 2084
LguI	GCTCTTC	1 cut(s)	1615
LmnI	GCTCC	7 cut(s)	16, 304, 380, 761, 795, 1154, 1901
Lsp1109I	GCAGC	4 cut(s)	138, 159, 394, 1953
MaeI	CTAG	5 cut(s)	534, 1148, 1413, 1509, 1667
MaeII	ACGT	7 cut(s)	521, 645, 738, 1195, 1758, 1800, 1809
MaeIII	GTNAC	4 cut(s)	646, 824, 835, 1907
MalI	GATC	7 cut(s)	20, 62, 480, 567, 708, 1773, 2086
MboI	GATC	7 cut(s)	18, 60, 478, 565, 706, 1771, 2084
MflI	RGATCY	2 cut(s)	478, 706
MhlI	GDGCHC	2 cut(s)	635, 1565
Mly113I	GGCGCC	1 cut(s)	87
MlyI	GAGTC	2 cut(s)	182, 245
MmeI	TCCRAC	3 cut(s)	38, 432, 1709
Mph1103I	ATGCAT	1 cut(s)	1956
MroNI	GCCGGC	1 cut(s)	662
MroXI	GAANNNNTTC	2 cut(s)	1208, 1608
MseI	TTAA	5 cut(s)	267, 362, 603, 642, 1392
MslI	CAYNNNNRTG	1 cut(s)	965
MspA1I	CMGCKG	3 cut(s)	679, 688, 1631
MspCI	CTTAAG	1 cut(s)	361
MspI	CCGG	4 cut(s)	321, 663, 715, 1051
MspR9I	CCNGG	5 cut(s)	35, 321, 476, 715, 716
Mva1269I	GAATGC	3 cut(s)	1510, 1542, 1956
MvaI	CCWGG	2 cut(s)	35, 476
MvnI	CGCG	4 cut(s)	213, 679, 752, 1079
MwoI	GCNNNNNNNGC	9 cut(s)	135, 144, 372, 668, 682, 685, 761, 1163, 1926
NaeI	GCCGGC	1 cut(s)	664
NarI	GGCGCC	1 cut(s)	87
NciI	CCSGG	3 cut(s)	321, 715, 716
NcoI	CCATGG	1 cut(s)	651
NdeII	GATC	7 cut(s)	18, 60, 478, 565, 706, 1771, 2084
NgoMIV	GCCGGC	1 cut(s)	662
NlaIII	CATG	9 cut(s)	497, 655, 670, 1321, 1597, 1862, 1958, 2017, 2050
NlaIV	GGNNCC	3 cut(s)	88, 480, 1599
NmuCI	GTSAC	4 cut(s)	646, 824, 835, 1907
NsiI	ATGCAT	1 cut(s)	1956
NspI	RCATGY	1 cut(s)	1958
NspV	TTCGAA	2 cut(s)	459, 1612
PaeR7I	CTCGAG	1 cut(s)	634
PagI	TCATGA	1 cut(s)	1858
PciSI	GCTCTTC	1 cut(s)	1615
PcsI	WCGNNNNNNNCGW	1 cut(s)	1806
PctI	GAATGC	3 cut(s)	1510, 1542, 1956
PdiI	GCCGGC	1 cut(s)	664
PdmI	GAANNNNTTC	2 cut(s)	1208, 1608
PfoI	TCCNGGA	1 cut(s)	474
PkrI	GCNGC	8 cut(s)	86, 128, 149, 384, 678, 687, 754, 1968
PleI	GAGTC	2 cut(s)	182, 245
PluTI	GGCGCC	1 cut(s)	90
PpsI	GAGTC	2 cut(s)	182, 245
Ppu21I	YACGTR	1 cut(s)	739
Psp6I	CCWGG	2 cut(s)	33, 474
PspFI	CCCAGC	1 cut(s)	1708
PspGI	CCWGG	2 cut(s)	33, 474
PspN4I	GGNNCC	3 cut(s)	88, 480, 1599
PspPI	GGNCC	2 cut(s)	48, 1531
PsuI	RGATCY	2 cut(s)	478, 706
PvuII	CAGCTG	1 cut(s)	1631
RsaI	GTAC	5 cut(s)	737, 1040, 1146, 1592, 1665
RsaNI	GTAC	5 cut(s)	736, 1039, 1145, 1591, 1664
RseI	CAYNNNNRTG	1 cut(s)	965
SacII	CCGCGG	1 cut(s)	680
SalI	GTCGAC	1 cut(s)	1996
SapI	GCTCTTC	1 cut(s)	1615
SaqAI	TTAA	5 cut(s)	267, 362, 603, 642, 1392
SatI	GCNGC	8 cut(s)	85, 127, 148, 383, 677, 686, 753, 1967
Sau3AI	GATC	7 cut(s)	18, 60, 478, 565, 706, 1771, 2084
Sau96I	GGNCC	2 cut(s)	48, 1531
ScaI	AGTACT	2 cut(s)	1040, 1665
SchI	GAGTC	2 cut(s)	182, 245
ScrFI	CCNGG	5 cut(s)	35, 321, 476, 715, 716
SduI	GDGCHC	2 cut(s)	635, 1565
SfoI	GGCGCC	1 cut(s)	88
Sfr274I	CTCGAG	1 cut(s)	634
Sfr303I	CCGCGG	1 cut(s)	680
SfuI	TTCGAA	2 cut(s)	459, 1612
SgrBI	CCGCGG	1 cut(s)	680
SinI	GGWCC	1 cut(s)	48
SlaI	CTCGAG	1 cut(s)	634
SmaI	CCCGGG	1 cut(s)	716
SmiMI	CAYNNNNRTG	1 cut(s)	965
SmlI	CTYRAG	3 cut(s)	361, 634, 1778
SmoI	CTYRAG	3 cut(s)	361, 634, 1778
SspDI	GGCGCC	1 cut(s)	86
SspMI	CTAG	5 cut(s)	534, 1148, 1413, 1509, 1667
StyD4I	CCNGG	5 cut(s)	33, 319, 474, 713, 714
StyI	CCWWGG	1 cut(s)	651
TaaI	ACNGT	1 cut(s)	329
TaiI	ACGT	7 cut(s)	524, 648, 741, 1198, 1761, 1803, 1812
TaqI	TCGA	7 cut(s)	444, 459, 635, 1226, 1612, 1812, 1997
TaqII	GACCGA	1 cut(s)	1705
TatI	WGTACW	2 cut(s)	1038, 1663
TauI	GCSGC	4 cut(s)	87, 679, 688, 755
Tru1I	TTAA	5 cut(s)	267, 362, 603, 642, 1392
Tru9I	TTAA	5 cut(s)	267, 362, 603, 642, 1392
TscAI	CASTG	3 cut(s)	210, 829, 1173
TseFI	GTSAC	4 cut(s)	646, 824, 835, 1907
TseI	GCWGC	4 cut(s)	126, 147, 382, 1966
Tsp45I	GTSAC	4 cut(s)	646, 824, 835, 1907
TspDTI	ATGAA	7 cut(s)	744, 949, 1007, 1517, 1814, 1901, 2063
TspMI	CCCGGG	1 cut(s)	714
TspRI	CASTG	3 cut(s)	210, 829, 1173
Vha464I	CTTAAG	1 cut(s)	361
VpaK11BI	GGWCC	1 cut(s)	48
XapI	RAATTY	5 cut(s)	844, 1176, 1252, 1960, 2053
XceI	RCATGY	1 cut(s)	1958
XcmI	CCANNNNNNNNNTGG	1 cut(s)	202
XhoI	CTCGAG	1 cut(s)	634
XmaI	CCCGGG	1 cut(s)	714
XmiI	GTMKAC	1 cut(s)	1997
XmnI	GAANNNNTTC	2 cut(s)	1208, 1608
XspI	CTAG	5 cut(s)	534, 1148, 1413, 1509, 1667
ZraI	GACGTC	1 cut(s)	1810
ZrmI	AGTACT	2 cut(s)	1040, 1665
Zsp2I	ATGCAT	1 cut(s)	1956

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.