MD17G1239200.v1.1

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014988
Physical Location & Seq
Reverse (-)
28860030 .. 28860522
493 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1239200.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGGGGTTGGGAAGCTAAGGTTTTGCATAGAGATATCAAAGCAAGCAATGTGCTACTTTGATAAGGATATGAATGCTAGGTTAGGGGATTTCGGATTGGTCCGAATGCACCAACATGGGGAAATGACTGGCACGACACAAGTGGTTGGGACGGCAGGGTACATGGCGCCAGAAGTGGTTCGAACGGGAAGAGCCTCAGCTGAGATTGATATAGGCAGGGAGCCGGGGTTGGTTGTTTCGGTATGGAGACTAATGGAGAGGGGAAAGTTGAACTTTGCACTTGATGAGCGGCTCAAGGCGAAGGGCGGATACAGCGTGGAGGAAGTTGAGAAGTTGCTTCATTTGGGTTTGTTGTGTGCATATCCAGAAGCTAATGGGAGGCCAACAATTACGCAAGTTATGAAGGTGCTTGAAGGAGCAAACGACGATGAATGGCATTGA

Protein Analysis

147

Amino Acids

16.21

Weight (kDa)

6.09

Isoelectric Point (pI)

20.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 20 - 71 6.8e-08 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014988)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28350
fragaria_vesca FvH4_6g26300
malus_domestica MD09G1246400.v1.1 MD17G1239200.v1.1 MD17G1239300.v1.1
prunus_persica Prupe.3G131900_v2.0.a1
pyrus_communis pycom09g16380
rosa_chinensis RchiOBHm_Chr2g0126741
rosa_laevigata RLG00000018918
rosa_roxburghii Rroxscaffold_2G00117000
rosa_rugosa Rorug02G0264500
rosa_samantha Rh2AG321600 Rh2BG329700 Rh2CG309400 Rh2DG349000
rosa_wichuraiana Rw2G026060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 166
AccBSI CCGCTC 1 cut(s) 289
AciI CCGC 2 cut(s) 289, 306
AcyI GRCGYC 1 cut(s) 167
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 1 cut(s) 118
AgsI TTSAA 2 cut(s) 271, 413
AluBI AGCT 3 cut(s) 16, 200, 371
AluI AGCT 3 cut(s) 16, 200, 371
Alw26I GTCTC 1 cut(s) 241
AoxI GGCC 1 cut(s) 380
Asp700I GAANNNNTTC 1 cut(s) 177
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 1 cut(s) 100
AsuC2I CCSGG 1 cut(s) 225
AsuII TTCGAA 1 cut(s) 181
AvaII GGWCC 1 cut(s) 100
BanI GGYRCC 1 cut(s) 166
BarI GAAGNNNNNNTAC 2 cut(s) 293, 325
BbvCI CCTCAGC 1 cut(s) 196
BceAI ACGGC 1 cut(s) 168
BciVI GTATCC 1 cut(s) 302
BcnI CCSGG 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 241
BfaI CTAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 170
BfuI GTATCC 1 cut(s) 302
BisI GCNGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 291
Bme1390I CCNGG 1 cut(s) 225
Bme18I GGWCC 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 2 cut(s) 168, 222
BmrFI CCNGG 1 cut(s) 225
Bpu10I CCTNAGC 2 cut(s) 17, 196
Bpu14I TTCGAA 1 cut(s) 181
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 224
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 54
BseDI CCNNGG 1 cut(s) 224
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 2 cut(s) 192, 210
BseNI ACTGG 1 cut(s) 133
BshFI GGCC 1 cut(s) 382
BshNI GGYRCC 1 cut(s) 166
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 163
BsmI GAATGC 2 cut(s) 79, 111
BsnI GGCC 1 cut(s) 382
Bsp119I TTCGAA 1 cut(s) 181
BspACI CCGC 2 cut(s) 289, 306
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 2 cut(s) 193, 209
BspLI GGNNCC 2 cut(s) 168, 222
BspQI GCTCTTC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 181
BspT107I GGYRCC 1 cut(s) 166
BsrBI CCGCTC 1 cut(s) 289
BsrDI GCAATG 1 cut(s) 54
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 224
BssNI GRCGYC 1 cut(s) 167
Bst6I CTCTTC 1 cut(s) 184
BstACI GRCGYC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 3 cut(s) 17, 196, 201
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 1 cut(s) 169
BstMAI GTCTC 1 cut(s) 241
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 223
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 1 cut(s) 382
Cac8I GCNNGC 1 cut(s) 45
CfoI GCGC 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 100
Csp6I GTAC 1 cut(s) 160
CviAII CATG 2 cut(s) 116, 163
CviJI RGCY 7 cut(s) 16, 194, 200, 223, 292, 371, 382
CviKI_1 RGCY 7 cut(s) 16, 194, 200, 223, 292, 371, 382
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 3 cut(s) 17, 196, 201
DinI GGCGCC 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
EciI GGCGGA 1 cut(s) 321
Eco32I GATATC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 100
EcoRV GATATC 1 cut(s) 36
EgeI GGCGCC 1 cut(s) 168
EheI GGCGCC 1 cut(s) 168
FaeI CATG 2 cut(s) 119, 166
FaiI YATR 8 cut(s) 29, 71, 117, 164, 212, 244, 361, 401
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 1 cut(s) 163
FatI CATG 2 cut(s) 115, 162
Fnu4HI GCNGC 1 cut(s) 290
Fsp4HI GCNGC 1 cut(s) 290
FspBI CTAG 1 cut(s) 78
GlaI GCGC 1 cut(s) 168
GluI GCNGC 1 cut(s) 290
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 1 cut(s) 382
HapII CCGG 1 cut(s) 224
HhaI GCGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 167
Hin1II CATG 2 cut(s) 119, 166
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HpaII CCGG 1 cut(s) 224
Hpy188I TCNGA 2 cut(s) 95, 104
Hpy188III TCNNGA 1 cut(s) 365
Hpy99I CGWCG 1 cut(s) 428
HpyAV CCTTC 3 cut(s) 295, 397, 407
HpyCH4V TGCA 4 cut(s) 27, 109, 278, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpyF3I CTNAG 3 cut(s) 17, 196, 201
Hsp92I GRCGYC 1 cut(s) 167
Hsp92II CATG 2 cut(s) 119, 166
HspAI GCGC 1 cut(s) 167
KasI GGCGCC 1 cut(s) 166
LguI GCTCTTC 1 cut(s) 184
LmnI GCTCC 2 cut(s) 220, 416
LpnPI CCDG 6 cut(s) 114, 141, 183, 202, 237, 378
MaeI CTAG 1 cut(s) 78
MbiI CCGCTC 1 cut(s) 289
MboII GAAGA 1 cut(s) 201
MluCI AATT 1 cut(s) 387
Mly113I GGCGCC 1 cut(s) 167
MnlI CCTC 4 cut(s) 205, 252, 313, 372
MroXI GAANNNNTTC 1 cut(s) 177
MslI CAYNNNNRTG 1 cut(s) 114
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 1 cut(s) 224
MspR9I CCNGG 1 cut(s) 225
Mva1269I GAATGC 2 cut(s) 79, 111
MwoI GCNNNNNNNGC 1 cut(s) 312
NarI GGCGCC 1 cut(s) 167
NciI CCSGG 1 cut(s) 225
NlaIII CATG 2 cut(s) 119, 166
NlaIV GGNNCC 2 cut(s) 168, 222
NspV TTCGAA 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 184
PctI GAATGC 2 cut(s) 79, 111
PdmI GAANNNNTTC 1 cut(s) 177
PkrI GCNGC 1 cut(s) 291
PluTI GGCGCC 1 cut(s) 170
PspN4I GGNNCC 2 cut(s) 168, 222
PspPI GGNCC 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
RseI CAYNNNNRTG 1 cut(s) 114
SapI GCTCTTC 1 cut(s) 184
SatI GCNGC 1 cut(s) 290
Sau96I GGNCC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 225
SetI ASST 6 cut(s) 18, 23, 83, 202, 373, 408
SfoI GGCGCC 1 cut(s) 168
SfuI TTCGAA 1 cut(s) 181
SinI GGWCC 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 1 cut(s) 387
SsiI CCGC 2 cut(s) 289, 306
SspDI GGCGCC 1 cut(s) 166
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 223
TaqI TCGA 1 cut(s) 181
TasI AATT 1 cut(s) 387
TauI GCSGC 1 cut(s) 292
TspDTI ATGAA 3 cut(s) 86, 329, 416
VpaK11BI GGWCC 1 cut(s) 100
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.