Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010358

Physical Location & Seq

Genomic Coordinates Reverse (-)

656604 .. 665562

Gene Structure 8959 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1003500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2787 bp

ATGTCTCTCAACATGAAAACCCTAACCCAAGCCTTCGCCAAGACCGCTGCAGCCATCGAAAAGACCGTGCAGACTACGGTCCAGGAAGTCGCCGGACCCAAGCCTCTCCAGGACTACGAGCTCTTCGATCAGATCGGGTCAGCCGGACCGGGCCTGGTCTGGAAGCTCTACTCGGCCAAGGCGGCTCGCGATTCCACGCGCGCGCACCAGTACCCCACCGTCTGCGTCTGGGTTTTGGATAAGAAGGCTCTGTCGGAGGCGCGCCTGCGCGCCGGATTGACCAAGGCGGCGGAAGATGGGTTTTTGGAGATTATTCGGGCTGATGCGGCGAGGCTGGTGAGGCTGAGGCATCCCGGGGTGGTGCACGTGGTGCAGGCGCTGGATGAGAACAAGAATGCCATGGCAATGGTGACGGAGCCATTGTTTGCGTCTGTGGCAAATGCGCTTGGGAATGTGGAGAATGTGGCTAAGGTGCCCAAGGAGCTTAAAGGAATGGAAATGGGTATATTGGAGGTGAAGCATGGTTTGCTCCAGTTAGCAGAATCCTTAGACTTTCTCCATAACAATGCGCGTCTCATTCATCGGGCTATATCACCTGAGAATGTCTTTATTACATCAAGTGGAGCTTGGAAGCTTGGTGGATTTGGTTTTGCAATCTCAACAGATCAAGCCTCAGGAAATATGACTAATGTTCAGGCATTCCATTATGCTGAATATGATGTTGAGGACTCTGTGCTGCCCATTCAGCCACCACTGAATTACACTGCTCCTGAAATTGCTAAGAGCAAAGCATCTTCAGTTGGATGTTCTTCTGATATTTTCAGTTTTGGTTGTCTTGCCTACCACTTGATTGCTCACAAGCCCTTGCTCGACTGCCACAACAATGTCAAGATGTACATGAATACCTTAAGTTACTTATCTAGTGAAGCCTTTTCCTCTATTCCTTCGGAGTTAGTTCCTGACTTGCAAAGGATGATTTCTACAAATGAGGCTTTCAGGCCAACAGCAATTGAATTTACAGGTTCTCCATTTTTCCGGGATGACACTAGGTTGCGTGCTCTTCGCTTTCTCGACCACATGCTTGAAAGAGATAACATGCAGAAGTCTGAATTCCTGAAAGCATTATCTGATATGTGGAAAGATTTCGATTCTCGAGTATTGCGATATAAGGTACTACCGCCGCTTTGTGCAGAACTTAGGAATTTGGTTATGCAACCAATGATTCTGCCCATGGTTCTCACGATTGCAGAGTCCCAGGATAAGAATGACTTTGAGTTATCAACATTGCCAGCCCTTGTTCCTGTCCTGAGCACCGCTGTGGGAGACACATTGTTGTTGCTTTTGAAGCATGCTGAACTTATAATCAACAAGACTATGCAAGATTACTTGATATCACACGTCCTGCCCATGATTGTTCGAGCATATGGTGATGCCGATGCTCGCATACAGGAGGAGGTTTTGAGAAAGTCTTCTTTCCTTGCTAATAAAATTGATGTTCAGCTAGTGAAACAAGCTATTCTGCCTCGTGTTCATGGCTTAGCTCTTAAGACAACTGTTGCCGCGGTGAGAGTCAATGCTTTGCTTTGTCTAGGAGATTTGATTCCTACACTCGATAAGAGTGCTATTTTGGATATTCTGCAAACAATTCAGCGTTGTACTGCAGTTGACCGTTCTGCTCCTACCCTTATGTGTACTCTTGGGGTGTCAAACTCAATTCTTAAGCAGCATGGGGTAGAATTTGTTGCAGAGCATGTCCTCCCACTGCTGATACCTCTCCTTACTGCCCAGCAATTAAATGTTCAGCAGTTTGCCAAATACATGCTCTTTGTCAAGGATATTCTGAGGAAGATAGAGGAAAAACGAGGAGTTACTGTGACGGATTCTGGAATTCCAGAGGTAAAACCATCTCCATCTGCCAATGGGCTCCACTCCCAAGTCCCAAGCAAAATTAGCGGAAATGTTGCCACCGCTGCAAACAGCAGTCCGGCATGGGATGAAGATTGGGGTCCCATCAGAAAGCAGCCCTCCAATTCCCTCCAAAATTCTACAAATTCCATTACCTCCACTTATCCCACTCTGGGTAATGAACCAATTCAAGTAACTTCTTCACAGCCAAATTCCTTGTTGAGAACTGCAGTATCTAGTCAGCAAACACCCGTGTCATGTTCCCCAGTTGATATAGAATGGCCTCCTCGAGCATCTTCTGGAGTTAACCCTGTTGCTGATGCTGAGAAGCAATTAAATGCAGGAACCTCATCCAGTTCCGGTTTTGATGACATAGATCCTTTTGCTAACTGGCCCCCACGGCCTAGTGGCCAAGTAAGTGGAACGGGCTCTTCCAACAATGGTACGATAGAGTCTCCCAGAAACAAATATGGTCCTAGTTCACTGTCCAGTACATCAAACAGTATGAACCTCTACAACAATAGCAATGACAGTTGGGCCTTTGGTACTGGAAGTTCTGTTGAGCAAATAGGACTTAATCAAGGCAATGCATCGAGTAGTGGTGGTTTGGGTAGCTCAGGTTTTAACCCCCAGAGTTCTATTGGATTTATGAAACAGAATCAACCGATAGCAGCTTCAAATGCTTACACAGATAAAAAATCAGCTGATCTTGGGTCCATATTTGCTTCTGGAAACAATGGGCAGACAGCACTTAGGCTTGCTCCTCCCCCATCAACTGCTGTAGGTCGAGGAAGAGGTAGAGGGAAGGGTGCTAGTTCAGCGTCACGCTCCAGCCATGCCAAATCGGCAACTGAACAGCCACCCCTTTTGGATCTGCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002090 GO:0002092 GO:0003674 GO:0005102 GO:0005488 GO:0005515 GO:0006810 GO:0006897 GO:0006898 GO:0007034 GO:0007041 GO:0007154 GO:0007165 GO:0007166 GO:0007267 GO:0008150 GO:0008152 GO:0008333 GO:0009893 GO:0009966 GO:0009968 GO:0009987 GO:0010604 GO:0010646 GO:0010648 GO:0016055 GO:0016192 GO:0019222 GO:0023051 GO:0023052 GO:0023057 GO:0030100 GO:0030111 GO:0030178 GO:0031323 GO:0031325 GO:0031623 GO:0032879 GO:0043112 GO:0043170 GO:0044237 GO:0044260 GO:0045807 GO:0046907 GO:0048259 GO:0048260 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060070 GO:0060255 GO:0060627 GO:0060828 GO:0065007 GO:0071704 GO:0090090 GO:0098657 GO:0198738 GO:1905114 GO:2000286 GO:2000369 GO:2000370

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

Pkinase

Protein Analysis

929

Amino Acids

100.21

Weight (kDa)

7.02

Isoelectric Point (pI)

44.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	105 - 345	4.4e-19	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	107 - 335	2.7e-10	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010358)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G22870 AT1G22870 AT1G22870 AT1G22870
fragaria_vesca	FvH4_2g00710
malus_domestica	MD05G1002800.v1.1 MD10G1003500.v1.1
prunus_persica	Prupe.8G006100_v2.0.a1
pyrus_communis	pycom05g00260 pycom10g00300
rosa_chinensis	RchiOBHm_Chr6g0251091
rosa_laevigata	RLG00000015485
rosa_multiflora	Rmu_sc0002219.1_g000007 Rmu_sc0024842.1_g000001
rosa_roxburghii	Rroxscaffold_7G00217050
rosa_rugosa	Rorug05G0500000
rosa_samantha	Rh6AG008200 Rh6BG002900 Rh6CG005700 Rh6DG005900
rosa_wichuraiana	Rw6G000910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1361
AccB1I	GGYRCC	1 cut(s)	472
AccII	CGCG	8 cut(s)	189, 199, 201, 203, 262, 270, 571, 1562
AclWI	GGATC	2 cut(s)	2276, 2784
AcoI	YGGCCR	2 cut(s)	174, 2314
AcsI	RAATTY	8 cut(s)	1013, 1109, 1201, 1736, 1887, 2041, 2050, 2116
AcuI	CTGAAG	1 cut(s)	780
AcvI	CACGTG	1 cut(s)	367
AdeI	CACNNNGTG	1 cut(s)	370
AfaI	GTAC	8 cut(s)	212, 896, 1173, 1657, 1693, 2350, 2398, 2452
AfiI	CCNNNNNNNGG	1 cut(s)	2078
AflII	CTTAAG	3 cut(s)	907, 1544, 1718
AflIII	ACRYGT	1 cut(s)	1396
AgsI	TTSAA	5 cut(s)	1013, 1085, 1345, 2096, 2583
AjiI	CACGTC	1 cut(s)	1399
AjnI	CCWGG	4 cut(s)	81, 108, 153, 1254
AleI	CACNNNNGTG	1 cut(s)	1316
Alw21I	GWGCWC	4 cut(s)	123, 366, 1060, 1313
Alw26I	GTCTC	4 cut(s)	9, 578, 1317, 2364
Alw44I	GTGCAC	1 cut(s)	362
AlwI	GGATC	2 cut(s)	2276, 2784
AlwNI	CAGNNNCTG	1 cut(s)	379
Ama87I	CYCGRG	3 cut(s)	353, 1152, 2193
AoxI	GGCC	8 cut(s)	151, 174, 998, 2186, 2297, 2306, 2314, 2442
ApaLI	GTGCAC	1 cut(s)	362
ApeKI	GCWGC	7 cut(s)	47, 50, 736, 1723, 1970, 2020, 2576
ApoI	RAATTY	8 cut(s)	1013, 1109, 1201, 1736, 1887, 2041, 2050, 2116
AscI	GGCGCGCC	1 cut(s)	260
Asp700I	GAANNNNTTC	3 cut(s)	1142, 1468, 2091
AspS9I	GGNCC	9 cut(s)	79, 95, 146, 151, 2006, 2298, 2378, 2442, 2619
AsuC2I	CCSGG	4 cut(s)	150, 354, 355, 1037
AsuHPI	GGTGA	6 cut(s)	349, 421, 526, 585, 1439, 1576
AvaI	CYCGRG	3 cut(s)	353, 1152, 2193
AvaII	GGWCC	6 cut(s)	79, 95, 146, 2006, 2378, 2619
AxyI	CCTNAGG	1 cut(s)	673
BaeGI	GKGCMC	2 cut(s)	366, 477
BaeI	ACNNNNGTAYC	2 cut(s)	2121, 2154
BalI	TGGCCA	1 cut(s)	2316
BanI	GGYRCC	1 cut(s)	472
BanII	GRGCYC	3 cut(s)	123, 1926, 2336
BauI	CACGAG	1 cut(s)	1524
BbrPI	CACGTG	1 cut(s)	367
BbsI	GAAGAC	1 cut(s)	1461
Bbv12I	GWGCWC	4 cut(s)	123, 366, 1060, 1313
BbvCI	CCTCAGC	1 cut(s)	344
BbvI	GCAGC	7 cut(s)	34, 62, 723, 1735, 1957, 2032, 2588
BccI	CCATC	6 cut(s)	62, 290, 1912, 1918, 2018, 2683
BceAI	ACGGC	1 cut(s)	2321
BcgI	CGANNNNNNTGC	2 cut(s)	1601, 1635
BciT130I	CCWGG	4 cut(s)	83, 110, 155, 1256
BcnI	CCSGG	4 cut(s)	150, 354, 355, 1037
BcoDI	GTCTC	4 cut(s)	9, 578, 1317, 2364
BfaI	CTAG	8 cut(s)	921, 1047, 1502, 1589, 2142, 2310, 2382, 2718
BfmI	CTRYAG	4 cut(s)	48, 1659, 2133, 2685
BfoI	RGCGCY	1 cut(s)	380
BfrI	CTTAAG	3 cut(s)	907, 1544, 1718
BglI	GCCNNNNNGGC	3 cut(s)	182, 2305, 2750
BlpI	GCTNAGC	1 cut(s)	1537
Bme1390I	CCNGG	8 cut(s)	83, 110, 150, 155, 354, 355, 1037, 1256
Bme18I	GGWCC	6 cut(s)	79, 95, 146, 2006, 2378, 2619
BmeT110I	CYCGRG	3 cut(s)	353, 1152, 2193
BmgBI	CACGTC	1 cut(s)	1399
BmgT120I	GGNCC	9 cut(s)	79, 95, 146, 151, 2006, 2298, 2378, 2442, 2619
BmiI	GGNNCC	9 cut(s)	97, 417, 474, 1925, 2007, 2008, 2251, 2300, 2620
BmrFI	CCNGG	8 cut(s)	83, 110, 150, 155, 354, 355, 1037, 1256
BmrI	ACTGGG	1 cut(s)	2165
BmsI	GCATC	8 cut(s)	313, 358, 800, 1420, 1426, 2207, 2215, 2504
BmuI	ACTGGG	1 cut(s)	2165
BoxI	GACNNNNGTC	1 cut(s)	77
BpiI	GAAGAC	1 cut(s)	1461
BpmI	CTGGAG	4 cut(s)	92, 515, 2226, 2719
Bpu10I	CCTNAGC	4 cut(s)	344, 468, 1307, 2521
Bpu1102I	GCTNAGC	1 cut(s)	1537
BpuMI	CCSGG	4 cut(s)	150, 354, 355, 1037
BsaAI	YACGTR	1 cut(s)	367
BsaWI	WCCGGW	1 cut(s)	2264
BsaXI	ACNNNNNCTCC	6 cut(s)	407, 437, 1009, 1039, 1857, 1887
Bsc4I	CCNNNNNNNGG	1 cut(s)	2078
Bse1I	ACTGG	7 cut(s)	208, 532, 2171, 2259, 2300, 2394, 2458
Bse21I	CCTNAGG	1 cut(s)	673
Bse3DI	GCAATG	4 cut(s)	411, 1283, 2437, 2497
BseBI	CCWGG	4 cut(s)	83, 110, 155, 1256
BseGI	GGATG	7 cut(s)	349, 388, 809, 978, 1045, 1999, 2255
BseLI	CCNNNNNNNGG	1 cut(s)	2078
BseMI	GCAATG	4 cut(s)	411, 1283, 2437, 2497
BseMII	CTCAG	7 cut(s)	335, 588, 687, 1298, 1832, 2220, 2535
BseNI	ACTGG	7 cut(s)	208, 532, 2171, 2259, 2300, 2394, 2458
BsePI	GCGCGC	4 cut(s)	199, 201, 260, 268
BseRI	GAGGAG	4 cut(s)	1466, 1878, 2181, 2658
BseSI	GKGCMC	2 cut(s)	366, 477
BseXI	GCAGC	7 cut(s)	34, 62, 723, 1735, 1957, 2032, 2588
BseYI	CCCAGC	1 cut(s)	1785
BsgI	GTGCAG	3 cut(s)	89, 392, 1209
Bsh1236I	CGCG	8 cut(s)	189, 199, 201, 203, 262, 270, 571, 1562
BshFI	GGCC	8 cut(s)	153, 176, 1000, 2188, 2299, 2308, 2316, 2444
BshNI	GGYRCC	1 cut(s)	472
BsiHKAI	GWGCWC	4 cut(s)	123, 366, 1060, 1313
BsiHKCI	CYCGRG	3 cut(s)	353, 1152, 2193
BsiSI	CCGG	8 cut(s)	93, 144, 149, 273, 354, 1036, 1985, 2265
BslFI	GGGAC	3 cut(s)	1237, 1922, 1992
BslI	CCNNNNNNNGG	1 cut(s)	2078
BsmAI	GTCTC	4 cut(s)	9, 578, 1317, 2364
BsmBI	CGTCTC	1 cut(s)	578
BsmFI	GGGAC	3 cut(s)	1237, 1922, 1992
BsmI	GAATGC	2 cut(s)	400, 698
BsnI	GGCC	8 cut(s)	153, 176, 1000, 2188, 2299, 2308, 2316, 2444
BsoBI	CYCGRG	3 cut(s)	353, 1152, 2193
Bsp1286I	GDGCHC	7 cut(s)	123, 366, 477, 1060, 1313, 1926, 2336
Bsp1407I	TGTACA	1 cut(s)	894
Bsp143I	GATC	6 cut(s)	127, 132, 664, 2281, 2611, 2776
Bsp1720I	GCTNAGC	1 cut(s)	1537
Bsp19I	CCATGG	2 cut(s)	399, 1230
Bsp68I	TCGCGA	1 cut(s)	189
BspANI	GGCC	8 cut(s)	153, 176, 1000, 2188, 2299, 2308, 2316, 2444
BspCNI	CTCAG	7 cut(s)	336, 589, 686, 1299, 1833, 2221, 2534
BspFNI	CGCG	8 cut(s)	189, 199, 201, 203, 262, 270, 571, 1562
BspLI	GGNNCC	9 cut(s)	97, 417, 474, 1925, 2007, 2008, 2251, 2300, 2620
BspMAI	CTGCAG	3 cut(s)	52, 1663, 2137
BspPI	GGATC	2 cut(s)	2276, 2784
BspQI	GCTCTTC	3 cut(s)	128, 1065, 2341
BspT107I	GGYRCC	1 cut(s)	472
BspTI	CTTAAG	3 cut(s)	907, 1544, 1718
BsrDI	GCAATG	4 cut(s)	411, 1283, 2437, 2497
BsrGI	TGTACA	1 cut(s)	894
BsrI	ACTGG	7 cut(s)	208, 532, 2171, 2259, 2300, 2394, 2458
BssHII	GCGCGC	4 cut(s)	199, 201, 260, 268
BssMI	GATC	6 cut(s)	127, 132, 664, 2281, 2611, 2776
BssSI	CACGAG	1 cut(s)	1524
BssT1I	CCWWGG	5 cut(s)	177, 282, 399, 477, 1230
Bst2BI	CACGAG	1 cut(s)	1524
Bst2UI	CCWGG	4 cut(s)	83, 110, 155, 1256
Bst4CI	ACNGT	9 cut(s)	67, 79, 220, 1555, 1670, 1873, 2391, 2408, 2438
Bst6I	CTCTTC	4 cut(s)	128, 1065, 2341, 2692
BstAFI	CTTAAG	3 cut(s)	907, 1544, 1718
BstAPI	GCANNNNNTGC	2 cut(s)	370, 526
BstAUI	TGTACA	1 cut(s)	894
BstBAI	YACGTR	1 cut(s)	367
BstDSI	CCRYGG	4 cut(s)	399, 1230, 1560, 2303
BstF5I	GGATG	7 cut(s)	349, 388, 809, 978, 1045, 1999, 2255
BstFNI	CGCG	8 cut(s)	189, 199, 201, 203, 262, 270, 571, 1562
BstH2I	RGCGCY	1 cut(s)	380
BstKTI	GATC	6 cut(s)	130, 135, 667, 2284, 2614, 2779
BstMAI	GTCTC	4 cut(s)	9, 578, 1317, 2364
BstMBI	GATC	6 cut(s)	127, 132, 664, 2281, 2611, 2776
BstNI	CCWGG	4 cut(s)	83, 110, 155, 1256
BstNSI	RCATGY	5 cut(s)	1081, 1099, 1352, 1754, 1822
BstPAI	GACNNNNGTC	1 cut(s)	77
BstSCI	CCNGG	8 cut(s)	81, 108, 148, 153, 352, 353, 1035, 1254
BstSFI	CTRYAG	4 cut(s)	48, 1659, 2133, 2685
BstSLI	GKGCMC	2 cut(s)	366, 477
BstUI	CGCG	8 cut(s)	189, 199, 201, 203, 262, 270, 571, 1562
BstV1I	GCAGC	7 cut(s)	34, 62, 723, 1735, 1957, 2032, 2588
BstV2I	GAAGAC	1 cut(s)	1461
BstX2I	RGATCY	2 cut(s)	2281, 2776
BstXI	CCANNNNNNTGG	2 cut(s)	406, 2324
BstYI	RGATCY	2 cut(s)	2281, 2776
Bsu36I	CCTNAGG	1 cut(s)	673
BsuRI	GGCC	8 cut(s)	153, 176, 1000, 2188, 2299, 2308, 2316, 2444
BtgI	CCRYGG	4 cut(s)	399, 1230, 1560, 2303
BtrI	CACGTC	1 cut(s)	1399
BtsCI	GGATG	7 cut(s)	349, 388, 809, 978, 1045, 1999, 2255
BtsI	GCAGTG	2 cut(s)	762, 1760
BtsIMutI	CAGTG	4 cut(s)	752, 762, 1760, 2387
BtuMI	TCGCGA	1 cut(s)	189
CaiI	CAGNNNCTG	1 cut(s)	379
Cfr13I	GGNCC	9 cut(s)	79, 95, 146, 151, 2006, 2298, 2378, 2442, 2619
Cfr42I	CCGCGG	1 cut(s)	1563
Cfr9I	CCCGGG	1 cut(s)	353
CpoI	CGGWCCG	1 cut(s)	146
CseI	GACGC	4 cut(s)	214, 417, 560, 2715
Csp6I	GTAC	8 cut(s)	211, 895, 1172, 1656, 1692, 2349, 2397, 2451
CspCI	CAANNNNNGTGG	1 cut(s)	2754
CspI	CGGWCCG	1 cut(s)	146
CviQI	GTAC	8 cut(s)	211, 895, 1172, 1656, 1692, 2349, 2397, 2451
DpnI	GATC	6 cut(s)	129, 134, 666, 2283, 2613, 2778
DpnII	GATC	6 cut(s)	127, 132, 664, 2281, 2611, 2776
DraIII	CACNNNGTG	1 cut(s)	370
EaeI	YGGCCR	2 cut(s)	174, 2314
Eam1104I	CTCTTC	4 cut(s)	128, 1065, 2341, 2692
EarI	CTCTTC	4 cut(s)	128, 1065, 2341, 2692
EciI	GGCGGA	1 cut(s)	305
Ecl136II	GAGCTC	1 cut(s)	121
Eco130I	CCWWGG	5 cut(s)	177, 282, 399, 477, 1230
Eco24I	GRGCYC	3 cut(s)	123, 1926, 2336
Eco32I	GATATC	1 cut(s)	1392
Eco47I	GGWCC	6 cut(s)	79, 95, 146, 2006, 2378, 2619
Eco53kI	GAGCTC	1 cut(s)	121
Eco57I	CTGAAG	1 cut(s)	780
Eco72I	CACGTG	1 cut(s)	367
Eco81I	CCTNAGG	1 cut(s)	673
Eco88I	CYCGRG	3 cut(s)	353, 1152, 2193
EcoICRI	GAGCTC	1 cut(s)	121
EcoO109I	RGGNCCY	1 cut(s)	2006
EcoRI	GAATTC	2 cut(s)	1109, 1887
EcoRII	CCWGG	4 cut(s)	81, 108, 153, 1254
EcoRV	GATATC	1 cut(s)	1392
EcoT14I	CCWWGG	5 cut(s)	177, 282, 399, 477, 1230
EcoT22I	ATGCAT	1 cut(s)	2497
EcoT38I	GRGCYC	3 cut(s)	123, 1926, 2336
ErhI	CCWWGG	5 cut(s)	177, 282, 399, 477, 1230
Esp3I	CGTCTC	1 cut(s)	578
FalI	AAGNNNNNCTT	4 cut(s)	610, 642, 1253, 1285
FaqI	GGGAC	3 cut(s)	1237, 1922, 1992
FauNDI	CATATG	1 cut(s)	1423
FokI	GGATG	7 cut(s)	336, 395, 816, 985, 1052, 2006, 2242
FriOI	GRGCYC	3 cut(s)	123, 1926, 2336
FspBI	CTAG	8 cut(s)	921, 1047, 1502, 1589, 2142, 2310, 2382, 2718
GsaI	CCCAGC	1 cut(s)	1789
GsuI	CTGGAG	4 cut(s)	92, 515, 2226, 2719
HaeII	RGCGCY	1 cut(s)	380
HaeIII	GGCC	8 cut(s)	153, 176, 1000, 2188, 2299, 2308, 2316, 2444
HapII	CCGG	8 cut(s)	93, 144, 149, 273, 354, 1036, 1985, 2265
HgaI	GACGC	4 cut(s)	214, 417, 560, 2715
HincII	GTYRAC	2 cut(s)	1666, 2212
HindII	GTYRAC	2 cut(s)	1666, 2212
HindIII	AAGCTT	1 cut(s)	632
HpaI	GTTAAC	1 cut(s)	2212
HpaII	CCGG	8 cut(s)	93, 144, 149, 273, 354, 1036, 1985, 2265
HphI	GGTGA	6 cut(s)	349, 421, 526, 585, 1439, 1576
Hpy166II	GTNNAC	5 cut(s)	364, 1666, 1692, 2212, 2387
Hpy188I	TCNGA	8 cut(s)	132, 256, 814, 949, 1108, 1129, 1842, 2015
Hpy8I	GTNNAC	5 cut(s)	364, 1666, 1692, 2212, 2387
HpyAV	CCTTC	4 cut(s)	43, 238, 954, 2704
HpyCH4III	ACNGT	9 cut(s)	67, 79, 220, 1555, 1670, 1873, 2391, 2408, 2438
HpyCH4IV	ACGT	2 cut(s)	366, 1398
HpySE526I	ACGT	2 cut(s)	366, 1398
KflI	GGGWCCC	1 cut(s)	2006
KspAI	GTTAAC	1 cut(s)	2212
KspI	CCGCGG	1 cut(s)	1563
Kzo9I	GATC	6 cut(s)	127, 132, 664, 2281, 2611, 2776
LguI	GCTCTTC	3 cut(s)	128, 1065, 2341
LmnI	GCTCC	9 cut(s)	415, 481, 534, 623, 772, 1681, 1929, 2671, 2738
Lsp1109I	GCAGC	7 cut(s)	34, 62, 723, 1735, 1957, 2032, 2588
LweI	GCATC	8 cut(s)	313, 358, 800, 1420, 1426, 2207, 2215, 2504
MaeI	CTAG	8 cut(s)	921, 1047, 1502, 1589, 2142, 2310, 2382, 2718
MaeII	ACGT	2 cut(s)	366, 1398
MaeIII	GTNAC	6 cut(s)	409, 911, 1867, 1873, 2098, 2727
MalI	GATC	6 cut(s)	129, 134, 666, 2283, 2613, 2778
MauBI	CGCGCGCG	1 cut(s)	199
MboI	GATC	6 cut(s)	127, 132, 664, 2281, 2611, 2776
MfeI	CAATTG	1 cut(s)	1008
MflI	RGATCY	2 cut(s)	2281, 2776
MhlI	GDGCHC	7 cut(s)	123, 366, 477, 1060, 1313, 1926, 2336
MlsI	TGGCCA	1 cut(s)	2316
MluNI	TGGCCA	1 cut(s)	2316
MlyI	GAGTC	4 cut(s)	722, 1259, 1578, 2366
MmeI	TCCRAC	3 cut(s)	234, 781, 2364
Mox20I	TGGCCA	1 cut(s)	2316
Mph1103I	ATGCAT	1 cut(s)	2497
MroXI	GAANNNNTTC	3 cut(s)	1142, 1468, 2091
MscI	TGGCCA	1 cut(s)	2316
MseI	TTAA	9 cut(s)	486, 908, 1545, 1719, 1793, 2211, 2240, 2481, 2529
MslI	CAYNNNNRTG	4 cut(s)	404, 564, 882, 1316
Msp20I	TGGCCA	1 cut(s)	2316
MspA1I	CMGCKG	5 cut(s)	47, 1316, 1562, 1970, 2609
MspCI	CTTAAG	3 cut(s)	907, 1544, 1718
MspI	CCGG	8 cut(s)	93, 144, 149, 273, 354, 1036, 1985, 2265
MspR9I	CCNGG	8 cut(s)	83, 110, 150, 155, 354, 355, 1037, 1256
MunI	CAATTG	1 cut(s)	1008
Mva1269I	GAATGC	2 cut(s)	400, 698
MvaI	CCWGG	4 cut(s)	83, 110, 155, 1256
MvnI	CGCG	8 cut(s)	189, 199, 201, 203, 262, 270, 571, 1562
NciI	CCSGG	4 cut(s)	150, 354, 355, 1037
NcoI	CCATGG	2 cut(s)	399, 1230
NdeI	CATATG	1 cut(s)	1423
NdeII	GATC	6 cut(s)	127, 132, 664, 2281, 2611, 2776
NlaIV	GGNNCC	9 cut(s)	97, 417, 474, 1925, 2007, 2008, 2251, 2300, 2620
NmeAIII	GCCGAG	1 cut(s)	152
NmuCI	GTSAC	3 cut(s)	409, 1873, 2727
NruI	TCGCGA	1 cut(s)	189
NsiI	ATGCAT	1 cut(s)	2497
NspI	RCATGY	5 cut(s)	1081, 1099, 1352, 1754, 1822
OliI	CACNNNNGTG	1 cut(s)	1316
PaeI	GCATGC	1 cut(s)	1352
PaeR7I	CTCGAG	2 cut(s)	1152, 2193
PalAI	GGCGCGCC	1 cut(s)	260
PauI	GCGCGC	4 cut(s)	199, 201, 260, 268
PciSI	GCTCTTC	3 cut(s)	128, 1065, 2341
PcsI	WCGNNNNNNNCGW	3 cut(s)	63, 123, 1159
PctI	GAATGC	2 cut(s)	400, 698
PdmI	GAANNNNTTC	3 cut(s)	1142, 1468, 2091
PfeI	GAWTC	7 cut(s)	191, 542, 1148, 1222, 1600, 1880, 2563
PfoI	TCCNGGA	3 cut(s)	81, 108, 1035
PleI	GAGTC	4 cut(s)	722, 1258, 1577, 2365
PmaCI	CACGTG	1 cut(s)	367
PmlI	CACGTG	1 cut(s)	367
PpsI	GAGTC	4 cut(s)	722, 1258, 1577, 2365
Ppu21I	YACGTR	1 cut(s)	367
PpuMI	RGGWCCY	1 cut(s)	2006
PshAI	GACNNNNGTC	1 cut(s)	77
PsiI	TTATAA	1 cut(s)	1361
Psp124BI	GAGCTC	1 cut(s)	123
Psp5II	RGGWCCY	1 cut(s)	2006
Psp6I	CCWGG	4 cut(s)	81, 108, 153, 1254
PspCI	CACGTG	1 cut(s)	367
PspFI	CCCAGC	1 cut(s)	1785
PspGI	CCWGG	4 cut(s)	81, 108, 153, 1254
PspN4I	GGNNCC	9 cut(s)	97, 417, 474, 1925, 2007, 2008, 2251, 2300, 2620
PspPI	GGNCC	9 cut(s)	79, 95, 146, 151, 2006, 2298, 2378, 2442, 2619
PspPPI	RGGWCCY	1 cut(s)	2006
PspXI	VCTCGAGB	1 cut(s)	2193
PsrI	GAACNNNNNNTAC	2 cut(s)	2443, 2475
PstI	CTGCAG	3 cut(s)	52, 1663, 2137
PstNI	CAGNNNCTG	1 cut(s)	379
PsuI	RGATCY	2 cut(s)	2281, 2776
PteI	GCGCGC	4 cut(s)	199, 201, 260, 268
PvuII	CAGCTG	1 cut(s)	2609
RruI	TCGCGA	1 cut(s)	189
RsaI	GTAC	8 cut(s)	212, 896, 1173, 1657, 1693, 2350, 2398, 2452
RsaNI	GTAC	8 cut(s)	211, 895, 1172, 1656, 1692, 2349, 2397, 2451
RseI	CAYNNNNRTG	4 cut(s)	404, 564, 882, 1316
Rsr2I	CGGWCCG	1 cut(s)	146
RsrII	CGGWCCG	1 cut(s)	146
SacI	GAGCTC	1 cut(s)	123
SacII	CCGCGG	1 cut(s)	1563
SapI	GCTCTTC	3 cut(s)	128, 1065, 2341
SaqAI	TTAA	9 cut(s)	486, 908, 1545, 1719, 1793, 2211, 2240, 2481, 2529
Sau3AI	GATC	6 cut(s)	127, 132, 664, 2281, 2611, 2776
Sau96I	GGNCC	9 cut(s)	79, 95, 146, 151, 2006, 2298, 2378, 2442, 2619
SchI	GAGTC	4 cut(s)	722, 1259, 1578, 2366
ScrFI	CCNGG	8 cut(s)	83, 110, 150, 155, 354, 355, 1037, 1256
SduI	GDGCHC	7 cut(s)	123, 366, 477, 1060, 1313, 1926, 2336
SfaNI	GCATC	8 cut(s)	313, 358, 800, 1420, 1426, 2207, 2215, 2504
SfcI	CTRYAG	4 cut(s)	48, 1659, 2133, 2685
SfiI	GGCCNNNNNGGCC	1 cut(s)	2305
Sfr274I	CTCGAG	2 cut(s)	1152, 2193
Sfr303I	CCGCGG	1 cut(s)	1563
SgrBI	CCGCGG	1 cut(s)	1563
SgsI	GGCGCGCC	1 cut(s)	260
SinI	GGWCC	6 cut(s)	79, 95, 146, 2006, 2378, 2619
SlaI	CTCGAG	2 cut(s)	1152, 2193
SmaI	CCCGGG	1 cut(s)	355
SmiMI	CAYNNNNRTG	4 cut(s)	404, 564, 882, 1316
SmlI	CTYRAG	5 cut(s)	907, 1152, 1544, 1718, 2193
SmoI	CTYRAG	5 cut(s)	907, 1152, 1544, 1718, 2193
SphI	GCATGC	1 cut(s)	1352
SspMI	CTAG	8 cut(s)	921, 1047, 1502, 1589, 2142, 2310, 2382, 2718
SstI	GAGCTC	1 cut(s)	123
StyD4I	CCNGG	8 cut(s)	81, 108, 148, 153, 352, 353, 1035, 1254
StyI	CCWWGG	5 cut(s)	177, 282, 399, 477, 1230
TaaI	ACNGT	9 cut(s)	67, 79, 220, 1555, 1670, 1873, 2391, 2408, 2438
TaiI	ACGT	2 cut(s)	369, 1401
TatI	WGTACW	4 cut(s)	894, 1655, 1691, 2396
TauI	GCSGC	5 cut(s)	185, 290, 329, 1183, 1562
TfiI	GAWTC	7 cut(s)	191, 542, 1148, 1222, 1600, 1880, 2563
Tru1I	TTAA	9 cut(s)	486, 908, 1545, 1719, 1793, 2211, 2240, 2481, 2529
Tru9I	TTAA	9 cut(s)	486, 908, 1545, 1719, 1793, 2211, 2240, 2481, 2529
TscAI	CASTG	4 cut(s)	759, 769, 1767, 2394
TseFI	GTSAC	3 cut(s)	409, 1873, 2727
TseI	GCWGC	7 cut(s)	47, 50, 736, 1723, 1970, 2020, 2576
Tsp45I	GTSAC	3 cut(s)	409, 1873, 2727
TspDTI	ATGAA	8 cut(s)	29, 569, 914, 1520, 2010, 2100, 2426, 2570
TspGWI	ACGGA	2 cut(s)	428, 1892
TspMI	CCCGGG	1 cut(s)	353
TspRI	CASTG	4 cut(s)	759, 769, 1767, 2394
Vha464I	CTTAAG	3 cut(s)	907, 1544, 1718
VneI	GTGCAC	1 cut(s)	362
VpaK11BI	GGWCC	6 cut(s)	79, 95, 146, 2006, 2378, 2619
XapI	RAATTY	8 cut(s)	1013, 1109, 1201, 1736, 1887, 2041, 2050, 2116
XceI	RCATGY	5 cut(s)	1081, 1099, 1352, 1754, 1822
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2543
XhoI	CTCGAG	2 cut(s)	1152, 2193
XmaI	CCCGGG	1 cut(s)	353
XmnI	GAANNNNTTC	3 cut(s)	1142, 1468, 2091
XspI	CTAG	8 cut(s)	921, 1047, 1502, 1589, 2142, 2310, 2382, 2718
Zsp2I	ATGCAT	1 cut(s)	2497

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.