MD10G1026400.v1.1

Protein of unknown function (DUF677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0017725
Physical Location & Seq
Forward (+)
3259033 .. 3261208
2176 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1026400.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGTGGGCAAAACTTAGAGCTTCTTCAAATATTGCAAAGGGGAAAGAAGTGATAAGGCAAGTTCGTAAAAGCTTCAATCTAAACGAGGAGTACCTAAGTGCATTTAGAACCAAATCCTATGCAGACTTCTTCAACAAAGCTCAGTTGCTTGTAAACGAGTCATCATCATCTCCTTTGAATTACAACCATGATCACAAATTGACAGAAGTCCTCCTAGAACCTGGTCAAGAAGCCATACCAGATATCCTTGACTCGGCAATTCTTTCGAAGGAACCTAAATTGAAGGGCCTTATGCTCAACTACTTTGACATAAGTGCTGAAGCCTCAAAAATATGCAGCCACCTCTTAAAAAGCATCAACCATGTCCAATCCAGTTACCACTTTGTTCAACAAGAACTTGATAAATTTGAAGAATACTCACCGGACAAAATCAAATCCATTATTTCGGAGCTAAACTTGCTCATACTTCAAAACAACATTAACCCTTTTTCAAACCCTAATAACCATGACTTTGAGCTCATTCATGAAAAGTACTCGTCGGTTTTGCACCACCTAAAGTCGATGAGGAAAAAGGTGTCGCGGAAGATTAAGCGCATTAAATGTTTCAAAAAGGCCACTGGGATTTGCATAACAGCAGCTTGTAGTTTGATTGCTATAACCGCTATTGTTTTAGCAGCACATACTCTCACTGCCTTGCTCATGGGGCCAGCTCTCATCAGCTTCCCATTTAAAAGCTTCAAGAAAAAACCGCGTAGCATTCCATTTTTGAGAAGTAGGATTCTTACCAAAGTTGGAGAACAACTTGATGTGGCAGCCAAAGGGATTTATATTTTGAATAGGGATTTCGACACGATGAGCCGACTTGTGGCAAGGCTTCATGATGAGGTTGAACACAACAAGGCGATGATCCGGTTCTGCTTGGAGAGGAGAGAGGATAAGTTTTCTTTGCAGGTTGTGAAGGAGCTTAAGAAAACTGATGTTGGATTCAGGAAGCAAGTGGAAGAGCTTCAAGAGCATGTGTATTTGTGTCTTGTAACAATCAATCGAGCTAGAGCTTTGGTGGTCAAGGAGATGACAAAATCTTGTGCAGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

366

Amino Acids

41.82

Weight (kDa)

9.47

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 56 - 356 6e-26 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017725)

Species Orthologous Gene IDs
malus_domestica MD10G1026400.v1.1
prunus_persica Prupe.8G032500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0275241
rosa_laevigata RLG00000013483
rosa_multiflora Rmu_sc0022221.1_g000007 Rmu_ssc0000041.1_g000027
rosa_roxburghii Rroxscaffold_7G00193520
rosa_rugosa Rorug06G0090500
rosa_samantha Rh6AG203800 Rh6CG209300 Rh6DG199300
rosa_wichuraiana Rw6G017700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 940
AccII CGCG 2 cut(s) 580, 751
AciI CCGC 3 cut(s) 580, 660, 749
AclWI GGATC 1 cut(s) 901
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 1 cut(s) 339
AfaI GTAC 2 cut(s) 92, 533
AfiI CCNNNNNNNGG 2 cut(s) 253, 865
AflII CTTAAG 1 cut(s) 965
AjnI CCWGG 1 cut(s) 220
Alw21I GWGCWC 1 cut(s) 519
AlwI GGATC 1 cut(s) 901
AoxI GGCC 3 cut(s) 286, 612, 704
ApeKI GCWGC 4 cut(s) 336, 635, 674, 812
ApoI RAATTY 1 cut(s) 404
Asp700I GAANNNNTTC 1 cut(s) 1005
AspLEI GCGC 1 cut(s) 594
AspS9I GGNCC 2 cut(s) 286, 704
AsuHPI GGTGA 1 cut(s) 411
AsuII TTCGAA 1 cut(s) 266
BanII GRGCYC 1 cut(s) 519
Bbv12I GWGCWC 1 cut(s) 519
BbvI GCAGC 4 cut(s) 348, 647, 686, 824
BcgI CGANNNNNNTGC 2 cut(s) 246, 280
BciT130I CCWGG 1 cut(s) 222
BclI TGATCA 1 cut(s) 190
BfaI CTAG 2 cut(s) 215, 1050
BfrI CTTAAG 1 cut(s) 965
BfuAI ACCTGC 1 cut(s) 940
BisI GCNGC 4 cut(s) 337, 636, 675, 813
BlsI GCNGC 4 cut(s) 338, 637, 676, 814
BmcAI AGTACT 1 cut(s) 533
Bme1390I CCNGG 1 cut(s) 222
BmgT120I GGNCC 2 cut(s) 286, 704
BmiI GGNNCC 2 cut(s) 273, 705
BmrFI CCNGG 1 cut(s) 222
BmrI ACTGGG 1 cut(s) 627
BmsI GCATC 1 cut(s) 363
BmuI ACTGGG 1 cut(s) 627
BoxI GACNNNNGTC 1 cut(s) 206
Bpu14I TTCGAA 1 cut(s) 266
BsaWI WCCGGW 2 cut(s) 421, 909
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 865
Bse1I ACTGG 2 cut(s) 372, 622
BseBI CCWGG 1 cut(s) 222
BseLI CCNNNNNNNGG 2 cut(s) 253, 865
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 2 cut(s) 372, 622
BseRI GAGGAG 2 cut(s) 101, 940
BseXI GCAGC 4 cut(s) 348, 647, 686, 824
Bsh1236I CGCG 2 cut(s) 580, 751
BshFI GGCC 3 cut(s) 288, 614, 706
BsiHKAI GWGCWC 1 cut(s) 519
BsiSI CCGG 2 cut(s) 422, 910
BslI CCNNNNNNNGG 2 cut(s) 253, 865
BsmI GAATGC 1 cut(s) 756
BsnI GGCC 3 cut(s) 288, 614, 706
Bsp119I TTCGAA 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 519
Bsp143I GATC 2 cut(s) 190, 906
BspACI CCGC 3 cut(s) 580, 660, 749
BspANI GGCC 3 cut(s) 288, 614, 706
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 2 cut(s) 580, 751
BspHI TCATGA 2 cut(s) 523, 877
BspLI GGNNCC 2 cut(s) 273, 705
BspMI ACCTGC 1 cut(s) 940
BspPI GGATC 1 cut(s) 901
BspQI GCTCTTC 1 cut(s) 996
BspT104I TTCGAA 1 cut(s) 266
BspTI CTTAAG 1 cut(s) 965
BsrI ACTGG 2 cut(s) 372, 622
BssMI GATC 2 cut(s) 190, 906
Bst2UI CCWGG 1 cut(s) 222
Bst6I CTCTTC 1 cut(s) 996
BstAFI CTTAAG 1 cut(s) 965
BstBI TTCGAA 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 708
BstDEI CTNAG 3 cut(s) 14, 95, 141
BstFNI CGCG 2 cut(s) 580, 751
BstHHI GCGC 1 cut(s) 594
BstKTI GATC 2 cut(s) 193, 909
BstMBI GATC 2 cut(s) 190, 906
BstMWI GCNNNNNNNGC 4 cut(s) 457, 659, 703, 1012
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 1019
BstPAI GACNNNNGTC 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 220
BstUI CGCG 2 cut(s) 580, 751
BstV1I GCAGC 4 cut(s) 348, 647, 686, 824
BsuRI GGCC 3 cut(s) 288, 614, 706
BtgZI GCGATG 1 cut(s) 917
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 2 cut(s) 615, 687
BveI ACCTGC 1 cut(s) 940
Cac8I GCNNGC 1 cut(s) 708
CciI TCATGA 2 cut(s) 523, 877
CfoI GCGC 1 cut(s) 594
Cfr13I GGNCC 2 cut(s) 286, 704
CsiI ACCWGGT 1 cut(s) 220
Csp6I GTAC 2 cut(s) 91, 532
CviAII CATG 7 cut(s) 188, 362, 506, 524, 700, 878, 1016
CviQI GTAC 2 cut(s) 91, 532
DdeI CTNAG 3 cut(s) 14, 95, 141
DpnI GATC 2 cut(s) 192, 908
DpnII GATC 2 cut(s) 190, 906
DraI TTTAAA 1 cut(s) 730
Eam1104I CTCTTC 1 cut(s) 996
EarI CTCTTC 1 cut(s) 996
Ecl136II GAGCTC 1 cut(s) 517
Eco24I GRGCYC 1 cut(s) 519
Eco32I GATATC 1 cut(s) 244
Eco53kI GAGCTC 1 cut(s) 517
Eco57I CTGAAG 1 cut(s) 339
EcoICRI GAGCTC 1 cut(s) 517
EcoO109I RGGNCCY 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 220
EcoRV GATATC 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 519
FaeI CATG 7 cut(s) 191, 365, 509, 527, 703, 881, 1019
FatI CATG 7 cut(s) 187, 361, 505, 523, 699, 877, 1015
FbaI TGATCA 1 cut(s) 190
Fnu4HI GCNGC 4 cut(s) 337, 636, 675, 813
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 4 cut(s) 337, 636, 675, 813
FspBI CTAG 2 cut(s) 215, 1050
GlaI GCGC 1 cut(s) 593
GluI GCNGC 4 cut(s) 337, 636, 675, 813
HaeIII GGCC 3 cut(s) 288, 614, 706
HapII CCGG 2 cut(s) 422, 910
HhaI GCGC 1 cut(s) 594
Hin1II CATG 7 cut(s) 191, 365, 509, 527, 703, 881, 1019
Hin6I GCGC 1 cut(s) 592
HinP1I GCGC 1 cut(s) 592
HindIII AAGCTT 2 cut(s) 70, 733
HinfI GANTC 4 cut(s) 158, 251, 778, 984
HpaII CCGG 2 cut(s) 422, 910
HphI GGTGA 1 cut(s) 411
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 1 cut(s) 448
Hpy188III TCNNGA 6 cut(s) 227, 524, 739, 878, 988, 1010
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 1 cut(s) 541
HpyAV CCTTC 3 cut(s) 262, 277, 952
HpyCH4V TGCA 8 cut(s) 35, 101, 122, 336, 547, 627, 949, 1088
HpyF10VI GCNNNNNNNGC 4 cut(s) 457, 659, 703, 1012
HpyF3I CTNAG 3 cut(s) 14, 95, 141
Hsp92II CATG 7 cut(s) 191, 365, 509, 527, 703, 881, 1019
HspAI GCGC 1 cut(s) 592
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 2 cut(s) 190, 906
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 2 cut(s) 448, 961
Lsp1109I GCAGC 4 cut(s) 348, 647, 686, 824
LweI GCATC 1 cut(s) 363
MabI ACCWGGT 1 cut(s) 220
MaeI CTAG 2 cut(s) 215, 1050
MaeIII GTNAC 2 cut(s) 374, 1033
MalI GATC 2 cut(s) 192, 908
MboI GATC 2 cut(s) 190, 906
MboII GAAGA 5 cut(s) 15, 121, 422, 595, 1013
MhlI GDGCHC 1 cut(s) 519
MluCI AATT 6 cut(s) 178, 197, 258, 278, 404, 1093
MlyI GAGTC 2 cut(s) 167, 245
MmeI TCCRAC 2 cut(s) 772, 961
MnlI CCTC 8 cut(s) 79, 221, 334, 353, 558, 877, 918, 925
MroXI GAANNNNTTC 1 cut(s) 1005
MseI TTAA 6 cut(s) 347, 480, 588, 597, 729, 966
MspCI CTTAAG 1 cut(s) 965
MspI CCGG 2 cut(s) 422, 910
MspR9I CCNGG 1 cut(s) 222
Mva1269I GAATGC 1 cut(s) 756
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 2 cut(s) 580, 751
MwoI GCNNNNNNNGC 4 cut(s) 457, 659, 703, 1012
NdeII GATC 2 cut(s) 190, 906
NlaIII CATG 7 cut(s) 191, 365, 509, 527, 703, 881, 1019
NlaIV GGNNCC 2 cut(s) 273, 705
NmeAIII GCCGAG 1 cut(s) 233
NspI RCATGY 1 cut(s) 1019
NspV TTCGAA 1 cut(s) 266
PagI TCATGA 2 cut(s) 523, 877
PciSI GCTCTTC 1 cut(s) 996
PctI GAATGC 1 cut(s) 756
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 2 cut(s) 778, 984
PkrI GCNGC 4 cut(s) 338, 637, 676, 814
PleI GAGTC 2 cut(s) 166, 245
PpsI GAGTC 2 cut(s) 166, 245
PshAI GACNNNNGTC 1 cut(s) 206
Psp124BI GAGCTC 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 2 cut(s) 273, 705
PspPI GGNCC 2 cut(s) 286, 704
RsaI GTAC 2 cut(s) 92, 533
RsaNI GTAC 2 cut(s) 91, 532
SacI GAGCTC 1 cut(s) 519
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 6 cut(s) 347, 480, 588, 597, 729, 966
SatI GCNGC 4 cut(s) 337, 636, 675, 813
Sau3AI GATC 2 cut(s) 190, 906
Sau96I GGNCC 2 cut(s) 286, 704
ScaI AGTACT 1 cut(s) 533
SchI GAGTC 2 cut(s) 167, 245
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 1 cut(s) 519
SexAI ACCWGGT 1 cut(s) 220
SfaNI GCATC 1 cut(s) 363
SfuI TTCGAA 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 965
SmoI CTYRAG 1 cut(s) 965
Sse9I AATT 6 cut(s) 178, 197, 258, 278, 404, 1093
SsiI CCGC 3 cut(s) 580, 660, 749
SspI AATATT 1 cut(s) 31
SspMI CTAG 2 cut(s) 215, 1050
SstI GAGCTC 1 cut(s) 519
StyD4I CCNGG 1 cut(s) 220
TaqI TCGA 4 cut(s) 266, 560, 846, 1045
TasI AATT 6 cut(s) 178, 197, 258, 278, 404, 1093
TatI WGTACW 1 cut(s) 531
TfiI GAWTC 2 cut(s) 778, 984
Tru1I TTAA 6 cut(s) 347, 480, 588, 597, 729, 966
Tru9I TTAA 6 cut(s) 347, 480, 588, 597, 729, 966
TscAI CASTG 2 cut(s) 622, 694
TseI GCWGC 4 cut(s) 336, 635, 674, 812
TspDTI ATGAA 3 cut(s) 512, 540, 866
TspRI CASTG 2 cut(s) 622, 694
Vha464I CTTAAG 1 cut(s) 965
XapI RAATTY 1 cut(s) 404
XceI RCATGY 1 cut(s) 1019
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 2 cut(s) 215, 1050
ZrmI AGTACT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.