Rmu_sc0022221.1_g000007

Protein of unknown function (DUF677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0022221.1
OG0017725
Physical Location & Seq
Forward (+)
18155 .. 19687
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0022221.1_g000007.1.cds

Sequence Viewer

Length: 1092 bp
atgtgggcaaaatttaaagcttcaaagattaaagggaaggaagctataagaaatgttcgaaaaagctttaatctaaacgaggagtaccggagtgcatttagaaccaagtcctatgcagacttcttcaataaagctcaactgattgtgagccagccatcatgttcttctaatcacgaccatgatcagaaatcctcagaaatcctcctagaacctggtcaagaatcaatcaccggtattcttgaatccgcaattctttcccacttaccagaattgaaaggcctcatgctcgagtactttgaaataagtgctgaggcatcaaaaatctgcacccacctcttgaaaagcatccaccatattcggtctaactaccacttcattgaacaagcacttgacaaagttgaggactattctcctgaaaaaataaaatcgattattttggagctacacttgttcattgtccaaaaccatggtaacccattttcaaatccgaataaccatgacttcaagctcatccatgacaagtactcttcatttttacagcacctaaagtcaatgagaaaaagggtggcaaggaaacttaagcttatcgagtatttcaagaaggcttctgggattttcctaacagtagcttgtagtttggttgctataactgctgttattctatcagctcatgcttttggtgccttgctcatgggaccagcccttttcaacttcccatttaagcgtttgaagaaaaagctcggtagttacattccatttttaaggagtagggttcttaccaaagctggagaacaacttgatgtggcagctaaggggacttatatattaaatagggatttcgacatgatgagtcgactggttgcaaggcttcatgatgaagttgaacacaacaagatgatgatacacttctgcttggagagaagacaggaaaagtattctttgcaagttgtcaaggagcttaagaagagtaatggcgggtttaggaagcaagtggaggagctccaagagcacgtatatctgtgtcttgtaactatcaatagagctagaactatggtgacaaaagagatgaccaaatcttgtatgagaaactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

363

Amino Acids

41.92

Weight (kDa)

9.62

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017725)

Species Orthologous Gene IDs
malus_domestica MD10G1026400.v1.1
prunus_persica Prupe.8G032500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0275241
rosa_laevigata RLG00000013483
rosa_multiflora Rmu_sc0022221.1_g000007 Rmu_ssc0000041.1_g000027
rosa_roxburghii Rroxscaffold_7G00193520
rosa_rugosa Rorug06G0090500
rosa_samantha Rh6AG203800 Rh6CG209300 Rh6DG199300
rosa_wichuraiana Rw6G017700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 680
AccI GTMKAC 1 cut(s) 853
AciI CCGC 2 cut(s) 246, 975
AcsI RAATTY 1 cut(s) 11
AfaI GTAC 3 cut(s) 86, 293, 524
AflII CTTAAG 2 cut(s) 578, 959
AgeI ACCGGT 1 cut(s) 230
AjnI CCWGG 1 cut(s) 211
Alw21I GWGCWC 2 cut(s) 1002, 1011
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 1 cut(s) 277
ApeKI GCWGC 1 cut(s) 806
ApoI RAATTY 1 cut(s) 11
AsiGI ACCGGT 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 695
AsuHPI GGTGA 2 cut(s) 220, 1066
AsuII TTCGAA 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 1 cut(s) 695
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 1002
BbsI GAAGAC 1 cut(s) 928
Bbv12I GWGCWC 2 cut(s) 1002, 1011
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 1 cut(s) 818
BccI CCATC 1 cut(s) 163
BciT130I CCWGG 1 cut(s) 213
BclI TGATCA 1 cut(s) 181
BfaI CTAG 2 cut(s) 206, 1044
BfrI CTTAAG 2 cut(s) 578, 959
BisI GCNGC 1 cut(s) 807
BlsI GCNGC 1 cut(s) 808
BmcAI AGTACT 2 cut(s) 293, 524
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 1 cut(s) 695
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 695
BmiI GGNNCC 2 cut(s) 682, 696
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 2 cut(s) 323, 354
BpiI GAAGAC 1 cut(s) 928
BpmI CTGGAG 1 cut(s) 807
Bpu10I CCTNAGC 2 cut(s) 309, 810
Bpu14I TTCGAA 1 cut(s) 58
Bsa29I ATCGAT 1 cut(s) 428
BsaAI YACGTR 1 cut(s) 1012
BsaJI CCNNGG 1 cut(s) 466
BsaWI WCCGGW 2 cut(s) 87, 230
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 1 cut(s) 861
BseBI CCWGG 1 cut(s) 213
BseCI ATCGAT 1 cut(s) 428
BseDI CCNNGG 1 cut(s) 466
BseGI GGATG 2 cut(s) 345, 510
BseMII CTCAG 2 cut(s) 207, 300
BseNI ACTGG 1 cut(s) 861
BseRI GAGGAG 2 cut(s) 95, 1010
BseXI GCAGC 1 cut(s) 818
BsgI GTGCAG 1 cut(s) 310
BshFI GGCC 1 cut(s) 279
BshNI GGYRCC 1 cut(s) 680
BshTI ACCGGT 1 cut(s) 230
BshVI ATCGAT 1 cut(s) 428
BsiHKAI GWGCWC 2 cut(s) 1002, 1011
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 2 cut(s) 88, 231
BslFI GGGAC 2 cut(s) 708, 829
BsmFI GGGAC 2 cut(s) 708, 829
BsnI GGCC 1 cut(s) 279
BsoBI CYCGRG 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 58
Bsp1286I GDGCHC 2 cut(s) 1002, 1011
Bsp143I GATC 1 cut(s) 181
Bsp19I CCATGG 1 cut(s) 466
BspACI CCGC 2 cut(s) 246, 975
BspANI GGCC 1 cut(s) 279
BspCNI CTCAG 2 cut(s) 206, 301
BspDI ATCGAT 1 cut(s) 428
BspHI TCATGA 1 cut(s) 871
BspLI GGNNCC 2 cut(s) 682, 696
BspT104I TTCGAA 1 cut(s) 58
BspT107I GGYRCC 1 cut(s) 680
BspTI CTTAAG 2 cut(s) 578, 959
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 1 cut(s) 861
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 466
BssMI GATC 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 466
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 625
Bst6I CTCTTC 2 cut(s) 532, 959
BstAFI CTTAAG 2 cut(s) 578, 959
BstBAI YACGTR 1 cut(s) 1012
BstBI TTCGAA 1 cut(s) 58
BstC8I GCNNGC 1 cut(s) 152
BstDEI CTNAG 3 cut(s) 193, 309, 810
BstDSI CCRYGG 1 cut(s) 466
BstEII GGTNACC 1 cut(s) 470
BstF5I GGATG 2 cut(s) 345, 510
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstMWI GCNNNNNNNGC 3 cut(s) 650, 680, 1006
BstNI CCWGG 1 cut(s) 213
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 211
BstV1I GCAGC 1 cut(s) 818
BstV2I GAAGAC 1 cut(s) 928
BstXI CCANNNNNNTGG 1 cut(s) 467
Bsu15I ATCGAT 1 cut(s) 428
BsuRI GGCC 1 cut(s) 279
BsuTUI ATCGAT 1 cut(s) 428
BtgI CCRYGG 1 cut(s) 466
BtsCI GGATG 2 cut(s) 345, 510
Cac8I GCNNGC 1 cut(s) 152
CciI TCATGA 1 cut(s) 871
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 695
ClaI ATCGAT 1 cut(s) 428
CsiI ACCWGGT 1 cut(s) 211
Csp6I GTAC 3 cut(s) 85, 292, 523
CspAI ACCGGT 1 cut(s) 230
CviQI GTAC 3 cut(s) 85, 292, 523
DdeI CTNAG 3 cut(s) 193, 309, 810
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
DraI TTTAAA 1 cut(s) 16
Eam1104I CTCTTC 2 cut(s) 532, 959
EarI CTCTTC 2 cut(s) 532, 959
Ecl136II GAGCTC 1 cut(s) 1000
Eco130I CCWWGG 1 cut(s) 466
Eco147I AGGCCT 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 1002
Eco47I GGWCC 1 cut(s) 695
Eco53kI GAGCTC 1 cut(s) 1000
Eco88I CYCGRG 1 cut(s) 287
Eco91I GGTNACC 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 1000
EcoO65I GGTNACC 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 1002
ErhI CCWWGG 1 cut(s) 466
FaqI GGGAC 2 cut(s) 708, 829
FauI CCCGC 1 cut(s) 968
FbaI TGATCA 1 cut(s) 181
FblI GTMKAC 1 cut(s) 853
Fnu4HI GCNGC 1 cut(s) 807
FokI GGATG 2 cut(s) 332, 497
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 1 cut(s) 807
FspBI CTAG 2 cut(s) 206, 1044
GluI GCNGC 1 cut(s) 807
GsuI CTGGAG 1 cut(s) 807
HaeIII GGCC 1 cut(s) 279
HapII CCGG 2 cut(s) 88, 231
HincII GTYRAC 1 cut(s) 854
HindII GTYRAC 1 cut(s) 854
HindIII AAGCTT 3 cut(s) 18, 64, 581
HinfI GANTC 3 cut(s) 221, 242, 850
HpaII CCGG 2 cut(s) 88, 231
HphI GGTGA 2 cut(s) 220, 1066
Hpy166II GTNNAC 1 cut(s) 854
Hpy188I TCNGA 3 cut(s) 186, 196, 489
Hpy188III TCNNGA 7 cut(s) 173, 218, 239, 337, 413, 598, 872
Hpy8I GTNNAC 1 cut(s) 854
HpyAV CCTTC 2 cut(s) 31, 595
HpyCH4III ACNGT 1 cut(s) 625
HpyCH4IV ACGT 1 cut(s) 1011
HpyCH4V TGCA 5 cut(s) 95, 116, 327, 863, 943
HpyF10VI GCNNNNNNNGC 3 cut(s) 650, 680, 1006
HpyF3I CTNAG 3 cut(s) 193, 309, 810
HpySE526I ACGT 1 cut(s) 1011
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 4 cut(s) 439, 955, 997, 1005
Lsp1109I GCAGC 1 cut(s) 818
LweI GCATC 2 cut(s) 323, 354
MabI ACCWGGT 1 cut(s) 211
MaeI CTAG 2 cut(s) 206, 1044
MaeII ACGT 1 cut(s) 1011
MaeIII GTNAC 4 cut(s) 470, 746, 1027, 1054
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 6 cut(s) 115, 156, 519, 742, 933, 976
MhlI GDGCHC 2 cut(s) 1002, 1011
MluCI AATT 3 cut(s) 11, 249, 269
MlyI GAGTC 1 cut(s) 859
MnlI CCTC 8 cut(s) 73, 202, 212, 290, 304, 344, 394, 988
MseI TTAA 8 cut(s) 15, 30, 69, 579, 720, 761, 827, 960
MslI CAYNNNNRTG 1 cut(s) 177
MspCI CTTAAG 2 cut(s) 578, 959
MspI CCGG 2 cut(s) 88, 231
MspR9I CCNGG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 213
MwoI GCNNNNNNNGC 3 cut(s) 650, 680, 1006
NcoI CCATGG 1 cut(s) 466
NdeII GATC 1 cut(s) 181
NlaIV GGNNCC 2 cut(s) 682, 696
NmuCI GTSAC 1 cut(s) 1054
NspV TTCGAA 1 cut(s) 58
PaeR7I CTCGAG 1 cut(s) 287
PagI TCATGA 1 cut(s) 871
PceI AGGCCT 1 cut(s) 279
PfeI GAWTC 2 cut(s) 221, 242
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 1 cut(s) 808
PleI GAGTC 1 cut(s) 858
PpsI GAGTC 1 cut(s) 858
Ppu21I YACGTR 1 cut(s) 1012
Psp124BI GAGCTC 1 cut(s) 1002
Psp6I CCWGG 1 cut(s) 211
PspEI GGTNACC 1 cut(s) 470
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 2 cut(s) 682, 696
PspPI GGNCC 1 cut(s) 695
PspXI VCTCGAGB 1 cut(s) 287
RsaI GTAC 3 cut(s) 86, 293, 524
RsaNI GTAC 3 cut(s) 85, 292, 523
RseI CAYNNNNRTG 1 cut(s) 177
SacI GAGCTC 1 cut(s) 1002
SalI GTCGAC 1 cut(s) 852
SaqAI TTAA 8 cut(s) 15, 30, 69, 579, 720, 761, 827, 960
SatI GCNGC 1 cut(s) 807
Sau3AI GATC 1 cut(s) 181
Sau96I GGNCC 1 cut(s) 695
ScaI AGTACT 2 cut(s) 293, 524
SchI GAGTC 1 cut(s) 859
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 2 cut(s) 1002, 1011
SexAI ACCWGGT 1 cut(s) 211
SfaNI GCATC 2 cut(s) 323, 354
Sfr274I CTCGAG 1 cut(s) 287
SfuI TTCGAA 1 cut(s) 58
SinI GGWCC 1 cut(s) 695
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 177
SmlI CTYRAG 3 cut(s) 287, 578, 959
SmoI CTYRAG 3 cut(s) 287, 578, 959
Sse9I AATT 3 cut(s) 11, 249, 269
SseBI AGGCCT 1 cut(s) 279
SsiI CCGC 2 cut(s) 246, 975
SspMI CTAG 2 cut(s) 206, 1044
SstI GAGCTC 1 cut(s) 1002
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 1 cut(s) 625
TaiI ACGT 1 cut(s) 1014
TaqI TCGA 6 cut(s) 58, 288, 428, 588, 840, 853
TaqII GACCGA 1 cut(s) 348
TasI AATT 3 cut(s) 11, 249, 269
TatI WGTACW 2 cut(s) 291, 522
TfiI GAWTC 2 cut(s) 221, 242
Tru1I TTAA 8 cut(s) 15, 30, 69, 579, 720, 761, 827, 960
Tru9I TTAA 8 cut(s) 15, 30, 69, 579, 720, 761, 827, 960
TseFI GTSAC 1 cut(s) 1054
TseI GCWGC 1 cut(s) 806
Tsp45I GTSAC 1 cut(s) 1054
TspDTI ATGAA 5 cut(s) 364, 442, 519, 860, 891
Vha464I CTTAAG 2 cut(s) 578, 959
VpaK11BI GGWCC 1 cut(s) 695
XapI RAATTY 1 cut(s) 11
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 853
XspI CTAG 2 cut(s) 206, 1044
ZrmI AGTACT 2 cut(s) 293, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.