Rw6G017700

Protein of unknown function (DUF677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017725
Physical Location & Seq
Forward (+)
35021943 .. 35023444
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017700.1

Sequence Viewer

Length: 1098 bp
ATGTGGGCAAAATTTAAAGCTTCAAAGATTAAAGGTATGTGGAAGGAAGCTATAAGAAATGTTCGAAAAAGCTTTAATCTAAACGAGGAGTACCGGAGTGCATTTAGAACCCAGTCCTATGCAGACTTCTTCAATAAAGCTCAACTGATTGTGAGCCAGCCATCATGTTCTTCTAATCACGACCATGATCAGAAATCCTCAGAAATCCTCCTAGAACCTGGTCAAGAATCCATCACCGGTATTCTTGAATCCGCAATTCTTTCCCACTTACCCGAATTGAAAGGCCTCATGCTCGAGTACTTTGAAATAAGTGCTGAGGCATCAAAAATCTGCACCCACCTCTTGAAAAGCATCCACCATATTCGGTCTAACTACCACTTCATTGAACAAGCACTTGACAAAGTTGAGGACTATTCTCCTGAAAAAATAAAATCGATTATTTTGGAGCTACACTTGTTCATTGTCCAAAACCATGGTAACCCATTTTCAAATCCGAATAACCATGACTTCAAGCTCATCCATGACAAGTACTCTTCATTTTTACAGCACCTAAAGTCAATGAGAAAAAGGGTGGCAAGGAAACTTAAGCTTATCGAGTATTTGAAGAAGGCTTCTGGGATTTTCCTAACAGTAGCTTGTAGTTTGGTTGCTATAACTGCTGTTATTCTATCAGCTCATGCTTTTGGTGCCTTGCTCATGGGACCAGCCCTTTTCAACTTCCCATTTAAGCGTTTGAAGAAAAAGCTCGGTAGTTACATTCCATTTTTAAGGAGTAGGGTTCTTACCAAAGCTGGAGAACAACTTGATGTGGCAGCTAAGGGGACTTATATATTAAATAGGGATTTCGACATGATGAGTCGACTGGTTTCAAGGCTTCATGATGAAGTTGAACACAACAAGATGATGATACACTTCTGCTTGGAGAGAAGACAGGAAAAGTATTCTTTGCAAGTTGTCAAGGAGCTTAAGAAGAGTAATGGCGGGTTTAGGAAGCAAGTGGAGGAGCTCCAAGAGCACGTATATCTGTGTCTTGTAACTATCAATAGAGCTAGAACTATGGTGACAAAAGAGATGACCAAATCTTGTATGAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

365

Amino Acids

42.22

Weight (kDa)

9.59

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 55 - 355 5.8e-26 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017725)

Species Orthologous Gene IDs
malus_domestica MD10G1026400.v1.1
prunus_persica Prupe.8G032500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0275241
rosa_laevigata RLG00000013483
rosa_multiflora Rmu_sc0022221.1_g000007 Rmu_ssc0000041.1_g000027
rosa_roxburghii Rroxscaffold_7G00193520
rosa_rugosa Rorug06G0090500
rosa_samantha Rh6AG203800 Rh6CG209300 Rh6DG199300
rosa_wichuraiana Rw6G017700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 686
AccI GTMKAC 1 cut(s) 859
AciI CCGC 2 cut(s) 252, 981
AcsI RAATTY 1 cut(s) 11
AfaI GTAC 3 cut(s) 92, 299, 530
AflII CTTAAG 2 cut(s) 584, 965
AgeI ACCGGT 1 cut(s) 236
AjnI CCWGG 1 cut(s) 217
Alw21I GWGCWC 2 cut(s) 1008, 1017
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 1 cut(s) 283
ApeKI GCWGC 1 cut(s) 812
ApoI RAATTY 1 cut(s) 11
AsiGI ACCGGT 1 cut(s) 236
AspS9I GGNCC 1 cut(s) 701
AsuHPI GGTGA 2 cut(s) 226, 1072
AsuII TTCGAA 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 1 cut(s) 701
BanI GGYRCC 1 cut(s) 686
BanII GRGCYC 1 cut(s) 1008
BbsI GAAGAC 1 cut(s) 934
Bbv12I GWGCWC 2 cut(s) 1008, 1017
BbvCI CCTCAGC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 824
BccI CCATC 2 cut(s) 169, 239
BciT130I CCWGG 1 cut(s) 219
BclI TGATCA 1 cut(s) 187
BfaI CTAG 2 cut(s) 212, 1050
BfrI CTTAAG 2 cut(s) 584, 965
BisI GCNGC 1 cut(s) 813
BlsI GCNGC 1 cut(s) 814
BmcAI AGTACT 2 cut(s) 299, 530
Bme1390I CCNGG 1 cut(s) 219
Bme18I GGWCC 1 cut(s) 701
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 701
BmiI GGNNCC 2 cut(s) 688, 702
BmrFI CCNGG 1 cut(s) 219
BmrI ACTGGG 1 cut(s) 106
BmsI GCATC 2 cut(s) 329, 360
BmuI ACTGGG 1 cut(s) 106
BpiI GAAGAC 1 cut(s) 934
BpmI CTGGAG 1 cut(s) 813
Bpu10I CCTNAGC 2 cut(s) 315, 816
Bpu14I TTCGAA 1 cut(s) 64
Bsa29I ATCGAT 1 cut(s) 434
BsaAI YACGTR 1 cut(s) 1018
BsaJI CCNNGG 1 cut(s) 472
BsaWI WCCGGW 2 cut(s) 93, 236
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 2 cut(s) 112, 867
BseBI CCWGG 1 cut(s) 219
BseCI ATCGAT 1 cut(s) 434
BseDI CCNNGG 1 cut(s) 472
BseGI GGATG 2 cut(s) 351, 516
BseMII CTCAG 2 cut(s) 213, 306
BseNI ACTGG 2 cut(s) 112, 867
BseRI GAGGAG 2 cut(s) 101, 1016
BseXI GCAGC 1 cut(s) 824
BsgI GTGCAG 1 cut(s) 316
BshFI GGCC 1 cut(s) 285
BshNI GGYRCC 1 cut(s) 686
BshTI ACCGGT 1 cut(s) 236
BshVI ATCGAT 1 cut(s) 434
BsiHKAI GWGCWC 2 cut(s) 1008, 1017
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 2 cut(s) 94, 237
BslFI GGGAC 2 cut(s) 714, 835
BsmFI GGGAC 2 cut(s) 714, 835
BsnI GGCC 1 cut(s) 285
BsoBI CYCGRG 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 64
Bsp1286I GDGCHC 2 cut(s) 1008, 1017
Bsp143I GATC 1 cut(s) 187
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 2 cut(s) 252, 981
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 2 cut(s) 212, 307
BspDI ATCGAT 1 cut(s) 434
BspHI TCATGA 1 cut(s) 877
BspLI GGNNCC 2 cut(s) 688, 702
BspT104I TTCGAA 1 cut(s) 64
BspT107I GGYRCC 1 cut(s) 686
BspTI CTTAAG 2 cut(s) 584, 965
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 2 cut(s) 112, 867
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 472
BssMI GATC 1 cut(s) 187
BssT1I CCWWGG 1 cut(s) 472
Bst2UI CCWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 631
Bst6I CTCTTC 2 cut(s) 538, 965
BstAFI CTTAAG 2 cut(s) 584, 965
BstBAI YACGTR 1 cut(s) 1018
BstBI TTCGAA 1 cut(s) 64
BstC8I GCNNGC 1 cut(s) 158
BstDEI CTNAG 3 cut(s) 199, 315, 816
BstDSI CCRYGG 1 cut(s) 472
BstEII GGTNACC 1 cut(s) 476
BstF5I GGATG 2 cut(s) 351, 516
BstKTI GATC 1 cut(s) 190
BstMBI GATC 1 cut(s) 187
BstMWI GCNNNNNNNGC 3 cut(s) 656, 686, 1012
BstNI CCWGG 1 cut(s) 219
BstPI GGTNACC 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 217
BstV1I GCAGC 1 cut(s) 824
BstV2I GAAGAC 1 cut(s) 934
BstXI CCANNNNNNTGG 1 cut(s) 473
Bsu15I ATCGAT 1 cut(s) 434
BsuRI GGCC 1 cut(s) 285
BsuTUI ATCGAT 1 cut(s) 434
BtgI CCRYGG 1 cut(s) 472
BtsCI GGATG 2 cut(s) 351, 516
Cac8I GCNNGC 1 cut(s) 158
CciI TCATGA 1 cut(s) 877
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 701
ClaI ATCGAT 1 cut(s) 434
CsiI ACCWGGT 1 cut(s) 217
Csp6I GTAC 3 cut(s) 91, 298, 529
CspAI ACCGGT 1 cut(s) 236
CviQI GTAC 3 cut(s) 91, 298, 529
DdeI CTNAG 3 cut(s) 199, 315, 816
DpnI GATC 1 cut(s) 189
DpnII GATC 1 cut(s) 187
DraI TTTAAA 1 cut(s) 16
Eam1104I CTCTTC 2 cut(s) 538, 965
EarI CTCTTC 2 cut(s) 538, 965
Ecl136II GAGCTC 1 cut(s) 1006
Eco130I CCWWGG 1 cut(s) 472
Eco147I AGGCCT 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 1008
Eco47I GGWCC 1 cut(s) 701
Eco53kI GAGCTC 1 cut(s) 1006
Eco88I CYCGRG 1 cut(s) 293
Eco91I GGTNACC 1 cut(s) 476
EcoICRI GAGCTC 1 cut(s) 1006
EcoO65I GGTNACC 1 cut(s) 476
EcoRII CCWGG 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 1008
ErhI CCWWGG 1 cut(s) 472
FaqI GGGAC 2 cut(s) 714, 835
FauI CCCGC 1 cut(s) 974
FbaI TGATCA 1 cut(s) 187
FblI GTMKAC 1 cut(s) 859
Fnu4HI GCNGC 1 cut(s) 813
FokI GGATG 2 cut(s) 338, 503
FriOI GRGCYC 1 cut(s) 1008
Fsp4HI GCNGC 1 cut(s) 813
FspBI CTAG 2 cut(s) 212, 1050
GluI GCNGC 1 cut(s) 813
GsuI CTGGAG 1 cut(s) 813
HaeIII GGCC 1 cut(s) 285
HapII CCGG 2 cut(s) 94, 237
HincII GTYRAC 1 cut(s) 860
HindII GTYRAC 1 cut(s) 860
HindIII AAGCTT 3 cut(s) 18, 70, 587
HinfI GANTC 3 cut(s) 227, 248, 856
HpaII CCGG 2 cut(s) 94, 237
HphI GGTGA 2 cut(s) 226, 1072
Hpy166II GTNNAC 1 cut(s) 860
Hpy188I TCNGA 3 cut(s) 192, 202, 495
Hpy188III TCNNGA 6 cut(s) 179, 224, 245, 343, 419, 878
Hpy8I GTNNAC 1 cut(s) 860
HpyAV CCTTC 2 cut(s) 37, 601
HpyCH4III ACNGT 1 cut(s) 631
HpyCH4IV ACGT 1 cut(s) 1017
HpyCH4V TGCA 4 cut(s) 101, 122, 333, 949
HpyF10VI GCNNNNNNNGC 3 cut(s) 656, 686, 1012
HpyF3I CTNAG 3 cut(s) 199, 315, 816
HpySE526I ACGT 1 cut(s) 1017
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 1 cut(s) 187
LmnI GCTCC 4 cut(s) 445, 961, 1003, 1011
Lsp1109I GCAGC 1 cut(s) 824
LweI GCATC 2 cut(s) 329, 360
MabI ACCWGGT 1 cut(s) 217
MaeI CTAG 2 cut(s) 212, 1050
MaeII ACGT 1 cut(s) 1017
MaeIII GTNAC 4 cut(s) 476, 752, 1033, 1060
MalI GATC 1 cut(s) 189
MboI GATC 1 cut(s) 187
MboII GAAGA 7 cut(s) 121, 162, 525, 616, 748, 939, 982
MhlI GDGCHC 2 cut(s) 1008, 1017
MluCI AATT 3 cut(s) 11, 255, 275
MlyI GAGTC 1 cut(s) 865
MnlI CCTC 8 cut(s) 79, 208, 218, 296, 310, 350, 400, 994
MseI TTAA 8 cut(s) 15, 30, 75, 585, 726, 767, 833, 966
MslI CAYNNNNRTG 1 cut(s) 183
MspCI CTTAAG 2 cut(s) 584, 965
MspI CCGG 2 cut(s) 94, 237
MspR9I CCNGG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 219
MwoI GCNNNNNNNGC 3 cut(s) 656, 686, 1012
NcoI CCATGG 1 cut(s) 472
NdeII GATC 1 cut(s) 187
NlaIV GGNNCC 2 cut(s) 688, 702
NmuCI GTSAC 1 cut(s) 1060
NspV TTCGAA 1 cut(s) 64
PaeR7I CTCGAG 1 cut(s) 293
PagI TCATGA 1 cut(s) 877
PceI AGGCCT 1 cut(s) 285
PfeI GAWTC 2 cut(s) 227, 248
PinAI ACCGGT 1 cut(s) 236
PkrI GCNGC 1 cut(s) 814
PleI GAGTC 1 cut(s) 864
PpsI GAGTC 1 cut(s) 864
Ppu21I YACGTR 1 cut(s) 1018
Psp124BI GAGCTC 1 cut(s) 1008
Psp6I CCWGG 1 cut(s) 217
PspEI GGTNACC 1 cut(s) 476
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 688, 702
PspPI GGNCC 1 cut(s) 701
PspXI VCTCGAGB 1 cut(s) 293
RsaI GTAC 3 cut(s) 92, 299, 530
RsaNI GTAC 3 cut(s) 91, 298, 529
RseI CAYNNNNRTG 1 cut(s) 183
SacI GAGCTC 1 cut(s) 1008
SalI GTCGAC 1 cut(s) 858
SaqAI TTAA 8 cut(s) 15, 30, 75, 585, 726, 767, 833, 966
SatI GCNGC 1 cut(s) 813
Sau3AI GATC 1 cut(s) 187
Sau96I GGNCC 1 cut(s) 701
ScaI AGTACT 2 cut(s) 299, 530
SchI GAGTC 1 cut(s) 865
ScrFI CCNGG 1 cut(s) 219
SduI GDGCHC 2 cut(s) 1008, 1017
SexAI ACCWGGT 1 cut(s) 217
SfaNI GCATC 2 cut(s) 329, 360
Sfr274I CTCGAG 1 cut(s) 293
SfuI TTCGAA 1 cut(s) 64
SinI GGWCC 1 cut(s) 701
SlaI CTCGAG 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 3 cut(s) 293, 584, 965
SmoI CTYRAG 3 cut(s) 293, 584, 965
Sse9I AATT 3 cut(s) 11, 255, 275
SseBI AGGCCT 1 cut(s) 285
SsiI CCGC 2 cut(s) 252, 981
SspMI CTAG 2 cut(s) 212, 1050
SstI GAGCTC 1 cut(s) 1008
StuI AGGCCT 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 217
StyI CCWWGG 1 cut(s) 472
TaaI ACNGT 1 cut(s) 631
TaiI ACGT 1 cut(s) 1020
TaqI TCGA 6 cut(s) 64, 294, 434, 594, 846, 859
TaqII GACCGA 1 cut(s) 354
TasI AATT 3 cut(s) 11, 255, 275
TatI WGTACW 2 cut(s) 297, 528
TfiI GAWTC 2 cut(s) 227, 248
Tru1I TTAA 8 cut(s) 15, 30, 75, 585, 726, 767, 833, 966
Tru9I TTAA 8 cut(s) 15, 30, 75, 585, 726, 767, 833, 966
TseFI GTSAC 1 cut(s) 1060
TseI GCWGC 1 cut(s) 812
Tsp45I GTSAC 1 cut(s) 1060
TspDTI ATGAA 5 cut(s) 370, 448, 525, 866, 897
Vha464I CTTAAG 2 cut(s) 584, 965
VpaK11BI GGWCC 1 cut(s) 701
XapI RAATTY 1 cut(s) 11
XhoI CTCGAG 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 859
XspI CTAG 2 cut(s) 212, 1050
ZrmI AGTACT 2 cut(s) 299, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.