MD10G1051800.v1.1

Copper-transporting ATPase PAA1, chloroplastic-like isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0019595
Physical Location & Seq
Forward (+)
6877268 .. 6880996
3729 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1051800.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGAGTCCACATTGTCCGCAGCGACGCCGACAACGGCAGCTCTCCTCGCCGTCTCCAAATCCCTCAACCGCCGACTGGAAAGGAGGACCTTTCTCAGTCAACGCCTCCCGAGTCGTCTTCTTGCTCGCAGGCACCTCACTTCGGTTCACACCAGCTACTACGGCTTTCAGGCCCCCAAACCTGATCCTTTCTCTTCCTCTTCTCTGAGAAGTGTCTGCGTCGCTGGCCCGCTTTTACGGCGCCGTTTAGAGTGCGTCGGTAGCTCCGCTTCTTCTTTTGCCTCCGGCGGTGGAAATGGTGGCTTTGGCGGAGAGAGTGGCGGCAGCGGCGGTGACGGCGAGGGAGGTTCTGGCGGCGGCGATGCCAAGTCCAAGGTTGCTGCTACTGGAGGCTATGAGGTTTCGGCGCTCTCCACTGATGTCATTGTTCTCGATGTTGGAGGAATGACATGTGGAGGATGTGCAGCCAGTGTGAAGCGAATTCTAGAAAATCAACCACAGGTATCGTCTGCGACTGTAAACCTCACAACTGAGACGGCAATTGTGTGGCCTGTGTCCGAAGCGAAGGCTACACCAAACTGGCAAAAGCAGTTAGGAGAGACACTTGCAAACCAATTGACCAGTTGCGGTTTCAAGTCTAACCTTCGCGTGGCAGGTCAGGGAGCTACGGAAGGAGATATATCACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

230

Amino Acids

23.38

Weight (kDa)

9.1

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 143 - 212 2.6e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019595)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33520 AT4G33520
malus_domestica MD05G1044800.v1.1 MD10G1051800.v1.1
pyrus_communis pycom10g03660
rosa_chinensis RchiOBHm_Chr6g0271811
rosa_samantha Rh6CG181000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 644
AccB1I GGYRCC 2 cut(s) 132, 240
AccII CGCG 1 cut(s) 648
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 1 cut(s) 480
AcyI GRCGYC 2 cut(s) 26, 241
AfiI CCNNNNNNNGG 3 cut(s) 76, 142, 649
AflIII ACRYGT 1 cut(s) 449
AgsI TTSAA 1 cut(s) 634
AluBI AGCT 4 cut(s) 41, 156, 264, 665
AluI AGCT 4 cut(s) 41, 156, 264, 665
Alw26I GTCTC 3 cut(s) 58, 527, 593
AlwI GGATC 1 cut(s) 179
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 3 cut(s) 171, 226, 548
ApeKI GCWGC 5 cut(s) 20, 38, 324, 380, 464
ApoI RAATTY 1 cut(s) 480
AspLEI GCGC 2 cut(s) 243, 409
AspS9I GGNCC 3 cut(s) 87, 172, 227
AsuHPI GGTGA 2 cut(s) 344, 675
AvaI CYCGRG 1 cut(s) 109
AvaII GGWCC 1 cut(s) 87
BanI GGYRCC 2 cut(s) 132, 240
BarI GAAGNNNNNNTAC 2 cut(s) 253, 285
BbsI GAAGAC 1 cut(s) 110
BbvI GCAGC 5 cut(s) 32, 50, 336, 367, 476
BceAI ACGGC 7 cut(s) 35, 51, 178, 228, 254, 352, 552
BcoDI GTCTC 3 cut(s) 58, 527, 593
BfaI CTAG 1 cut(s) 485
BfoI RGCGCY 2 cut(s) 244, 410
BfuAI ACCTGC 1 cut(s) 644
BisI GCNGC 9 cut(s) 21, 39, 322, 325, 328, 355, 358, 381, 465
BlsI GCNGC 9 cut(s) 22, 40, 323, 326, 329, 356, 359, 382, 466
Bme18I GGWCC 1 cut(s) 87
BmeT110I CYCGRG 1 cut(s) 109
BmgT120I GGNCC 3 cut(s) 87, 172, 227
BmiI GGNNCC 3 cut(s) 134, 174, 242
BmsI GCATC 1 cut(s) 352
BpiI GAAGAC 1 cut(s) 110
BpmI CTGGAG 1 cut(s) 408
BsaHI GRCGYC 2 cut(s) 26, 241
BsaJI CCNNGG 1 cut(s) 372
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 142, 649
Bse1I ACTGG 5 cut(s) 81, 391, 468, 584, 621
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 1 cut(s) 464
BseLI CCNNNNNNNGG 3 cut(s) 76, 142, 649
BseMII CTCAG 3 cut(s) 109, 197, 522
BseNI ACTGG 5 cut(s) 81, 391, 468, 584, 621
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 5 cut(s) 32, 50, 336, 367, 476
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 648
BshFI GGCC 3 cut(s) 173, 228, 550
BshNI GGYRCC 2 cut(s) 132, 240
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 1 cut(s) 285
BslI CCNNNNNNNGG 3 cut(s) 76, 142, 649
BsmAI GTCTC 3 cut(s) 58, 527, 593
BsmBI CGTCTC 2 cut(s) 58, 527
BsnI GGCC 3 cut(s) 173, 228, 550
BsoBI CYCGRG 1 cut(s) 109
Bsp143I GATC 1 cut(s) 184
BspANI GGCC 3 cut(s) 173, 228, 550
BspCNI CTCAG 3 cut(s) 108, 198, 523
BspFNI CGCG 1 cut(s) 648
BspLI GGNNCC 3 cut(s) 134, 174, 242
BspMI ACCTGC 1 cut(s) 644
BspPI GGATC 1 cut(s) 179
BspT107I GGYRCC 2 cut(s) 132, 240
BsrI ACTGG 5 cut(s) 81, 391, 468, 584, 621
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 1 cut(s) 184
BssNI GRCGYC 2 cut(s) 26, 241
BssT1I CCWWGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 517
Bst6I CTCTTC 2 cut(s) 199, 205
BstACI GRCGYC 2 cut(s) 26, 241
BstC8I GCNNGC 4 cut(s) 127, 131, 226, 230
BstDEI CTNAG 3 cut(s) 95, 206, 531
BstF5I GGATG 1 cut(s) 464
BstFNI CGCG 1 cut(s) 648
BstH2I RGCGCY 2 cut(s) 244, 410
BstHHI GCGC 2 cut(s) 243, 409
BstKTI GATC 1 cut(s) 187
BstMAI GTCTC 3 cut(s) 58, 527, 593
BstMBI GATC 1 cut(s) 184
BstMWI GCNNNNNNNGC 7 cut(s) 47, 162, 225, 238, 261, 327, 336
BstNSI RCATGY 1 cut(s) 453
BstUI CGCG 1 cut(s) 648
BstV1I GCAGC 5 cut(s) 32, 50, 336, 367, 476
BstV2I GAAGAC 1 cut(s) 110
BsuRI GGCC 3 cut(s) 173, 228, 550
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 1 cut(s) 464
BtsIMutI CAGTG 2 cut(s) 414, 475
BveI ACCTGC 1 cut(s) 644
Cac8I GCNNGC 4 cut(s) 127, 131, 226, 230
CfoI GCGC 2 cut(s) 243, 409
Cfr13I GGNCC 3 cut(s) 87, 172, 227
CseI GACGC 3 cut(s) 34, 208, 244
CviAII CATG 1 cut(s) 450
DdeI CTNAG 3 cut(s) 95, 206, 531
DinI GGCGCC 1 cut(s) 242
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
Eam1104I CTCTTC 2 cut(s) 199, 205
EarI CTCTTC 2 cut(s) 199, 205
EciI GGCGGA 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 109
EcoO109I RGGNCCY 2 cut(s) 87, 172
EcoRI GAATTC 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 372
EgeI GGCGCC 1 cut(s) 242
EheI GGCGCC 1 cut(s) 242
ErhI CCWWGG 1 cut(s) 372
Esp3I CGTCTC 2 cut(s) 58, 527
FaeI CATG 1 cut(s) 453
FaiI YATR 4 cut(s) 396, 451, 680, 688
FatI CATG 1 cut(s) 449
FauI CCCGC 1 cut(s) 237
Fnu4HI GCNGC 9 cut(s) 21, 39, 322, 325, 328, 355, 358, 381, 465
FokI GGATG 1 cut(s) 471
Fsp4HI GCNGC 9 cut(s) 21, 39, 322, 325, 328, 355, 358, 381, 465
FspBI CTAG 1 cut(s) 485
GlaI GCGC 2 cut(s) 242, 408
GluI GCNGC 9 cut(s) 21, 39, 322, 325, 328, 355, 358, 381, 465
GsuI CTGGAG 1 cut(s) 408
HaeII RGCGCY 2 cut(s) 244, 410
HaeIII GGCC 3 cut(s) 173, 228, 550
HapII CCGG 1 cut(s) 285
HgaI GACGC 3 cut(s) 34, 208, 244
HhaI GCGC 2 cut(s) 243, 409
Hin1I GRCGYC 2 cut(s) 26, 241
Hin1II CATG 1 cut(s) 453
Hin6I GCGC 2 cut(s) 241, 407
HinP1I GCGC 2 cut(s) 241, 407
HincII GTYRAC 1 cut(s) 101
HindII GTYRAC 1 cut(s) 101
HinfI GANTC 2 cut(s) 5, 112
HpaII CCGG 1 cut(s) 285
HphI GGTGA 2 cut(s) 344, 675
Hpy166II GTNNAC 4 cut(s) 9, 101, 148, 520
Hpy188I TCNGA 2 cut(s) 207, 559
Hpy188III TCNNGA 3 cut(s) 109, 431, 485
Hpy8I GTNNAC 4 cut(s) 9, 101, 148, 520
Hpy99I CGWCG 3 cut(s) 28, 224, 260
HpyAV CCTTC 3 cut(s) 559, 653, 665
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4V TGCA 2 cut(s) 464, 608
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 162, 225, 238, 261, 327, 336
HpyF3I CTNAG 3 cut(s) 95, 206, 531
Hsp92I GRCGYC 2 cut(s) 26, 241
Hsp92II CATG 1 cut(s) 453
HspAI GCGC 2 cut(s) 241, 407
KasI GGCGCC 1 cut(s) 240
Kzo9I GATC 1 cut(s) 184
LmnI GCTCC 2 cut(s) 269, 662
Lsp1109I GCAGC 5 cut(s) 32, 50, 336, 367, 476
LweI GCATC 1 cut(s) 352
MaeI CTAG 1 cut(s) 485
MaeIII GTNAC 1 cut(s) 332
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MboII GAAGA 4 cut(s) 110, 186, 192, 264
MfeI CAATTG 2 cut(s) 540, 614
MluCI AATT 3 cut(s) 480, 540, 614
Mly113I GGCGCC 1 cut(s) 241
MlyI GAGTC 2 cut(s) 14, 121
MmeI TCCRAC 1 cut(s) 418
MspA1I CMGCKG 1 cut(s) 327
MspI CCGG 1 cut(s) 285
MunI CAATTG 2 cut(s) 540, 614
MvnI CGCG 1 cut(s) 648
MwoI GCNNNNNNNGC 7 cut(s) 47, 162, 225, 238, 261, 327, 336
NarI GGCGCC 1 cut(s) 241
NdeII GATC 1 cut(s) 184
NlaIII CATG 1 cut(s) 453
NlaIV GGNNCC 3 cut(s) 134, 174, 242
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 1 cut(s) 453
PciI ACATGT 1 cut(s) 449
PkrI GCNGC 9 cut(s) 22, 40, 323, 326, 329, 356, 359, 382, 466
PleI GAGTC 2 cut(s) 13, 120
PluTI GGCGCC 1 cut(s) 244
PpsI GAGTC 2 cut(s) 13, 120
PpuMI RGGWCCY 1 cut(s) 87
PscI ACATGT 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 87
PspN4I GGNNCC 3 cut(s) 134, 174, 242
PspPI GGNCC 3 cut(s) 87, 172, 227
PspPPI RGGWCCY 1 cut(s) 87
SatI GCNGC 9 cut(s) 21, 39, 322, 325, 328, 355, 358, 381, 465
Sau3AI GATC 1 cut(s) 184
Sau96I GGNCC 3 cut(s) 87, 172, 227
SchI GAGTC 2 cut(s) 14, 121
SfaNI GCATC 1 cut(s) 352
SfoI GGCGCC 1 cut(s) 242
SinI GGWCC 1 cut(s) 87
Sse9I AATT 3 cut(s) 480, 540, 614
SspDI GGCGCC 1 cut(s) 240
SspMI CTAG 1 cut(s) 485
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 517
TaqI TCGA 1 cut(s) 432
TasI AATT 3 cut(s) 480, 540, 614
TauI GCSGC 4 cut(s) 324, 330, 357, 360
TscAI CASTG 2 cut(s) 421, 475
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 5 cut(s) 20, 38, 324, 380, 464
Tsp45I GTSAC 1 cut(s) 332
TspGWI ACGGA 1 cut(s) 683
TspRI CASTG 2 cut(s) 421, 475
VpaK11BI GGWCC 1 cut(s) 87
XapI RAATTY 1 cut(s) 480
XbaI TCTAGA 1 cut(s) 484
XceI RCATGY 1 cut(s) 453
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.