MD10G1057300.v1.1

DNA-directed RNA polymerases I, II, and III subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015909
Physical Location & Seq
Forward (+)
7802198 .. 7804024
1827 bp
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UTR
Exon/CDS
Intron
MD10G1057300.v1.1.491

Sequence Viewer

Length: 216 bp
ATGATCATCCCAGTGCGCTGTTTCACCTGCGGCAAGGTTATCGGAAACAAATGGGATACGTATCTCGACCTGCTGCAGGCCGATTACACAGAAGGAGACGCACTAGATGCATTGGGTCTGGTCCGCTACTGCTGCCGGCGTATGCTTATGACTCACGTTGATCTTATCGAGAAGCTTCTCAACTACAATACTTTGGAGAAGTCTGACGCCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05016 ko05169 map00230 map00240 map01100 map03020 map04623 map05016 map05169
Pfam Domains
Protein Families
RNA_pol_N

Protein Analysis

72

Amino Acids

8.13

Weight (kDa)

5.11

Isoelectric Point (pI)

22.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_N PF01194 2 - 59 3.1e-32 RNA polymerases N / 8 kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015909)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11475
fragaria_vesca FvH4_4g32930 FvH4_4g32930
malus_domestica MD05G1050000.v1.1 MD10G1057300.v1.1
prunus_persica Prupe.1G250300_v2.0.a1 Prupe.1G250300_v2.0.a1
pyrus_communis pycom05g04190 pycom10g04420
rosa_chinensis RchiOBHm_Chr5g0051151
rosa_multiflora Rmu_sc0000446.1_g000014
rosa_roxburghii Rroxscaffold_1G00029300
rosa_rugosa Rorug05G0266000
rosa_wichuraiana Rw0G023650 Rw5G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 35
Acc36I ACCTGC 2 cut(s) 35, 78
AciI CCGC 2 cut(s) 30, 124
AcyI GRCGYC 1 cut(s) 207
AfiI CCNNNNNNNGG 1 cut(s) 76
AluBI AGCT 1 cut(s) 175
AluI AGCT 1 cut(s) 175
Alw26I GTCTC 1 cut(s) 90
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 2 cut(s) 73, 132
AspLEI GCGC 1 cut(s) 18
AspS9I GGNCC 1 cut(s) 121
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 1 cut(s) 121
BbvI GCAGC 2 cut(s) 60, 119
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciVI GTATCC 1 cut(s) 49
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 104
BfmI CTRYAG 1 cut(s) 74
BfuAI ACCTGC 2 cut(s) 35, 78
BfuI GTATCC 1 cut(s) 49
BisI GCNGC 3 cut(s) 31, 74, 133
BlsI GCNGC 3 cut(s) 32, 75, 134
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 121
BmrI ACTGGG 1 cut(s) 5
BmsI GCATC 1 cut(s) 97
BmuI ACTGGG 1 cut(s) 5
BsaAI YACGTR 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 207
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 1 cut(s) 135
Bse1I ACTGG 2 cut(s) 11, 210
Bse8I GATNNNNATC 1 cut(s) 60
BseGI GGATG 1 cut(s) 6
BseJI GATNNNNATC 1 cut(s) 60
BseLI CCNNNNNNNGG 1 cut(s) 76
BseNI ACTGG 2 cut(s) 11, 210
BseXI GCAGC 2 cut(s) 60, 119
BshFI GGCC 1 cut(s) 80
BsiSI CCGG 1 cut(s) 136
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 90
BsmBI CGTCTC 1 cut(s) 90
BsnI GGCC 1 cut(s) 80
Bsp143I GATC 2 cut(s) 3, 160
BspACI CCGC 2 cut(s) 30, 124
BspANI GGCC 1 cut(s) 80
BspMAI CTGCAG 1 cut(s) 78
BspMI ACCTGC 2 cut(s) 35, 78
BsrFI RCCGGY 1 cut(s) 135
BsrI ACTGG 2 cut(s) 11, 210
BssAI RCCGGY 1 cut(s) 135
BssMI GATC 2 cut(s) 3, 160
BssNI GRCGYC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 107
BstBAI YACGTR 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 78, 137
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 1 cut(s) 6
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 2 cut(s) 6, 163
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 2 cut(s) 3, 160
BstMWI GCNNNNNNNGC 2 cut(s) 107, 132
BstSFI CTRYAG 1 cut(s) 74
BstSNI TACGTA 1 cut(s) 60
BstV1I GCAGC 2 cut(s) 60, 119
BsuI GTATCC 1 cut(s) 49
BsuRI GGCC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 6
BtsIMutI CAGTG 1 cut(s) 18
BveI ACCTGC 2 cut(s) 35, 78
Cac8I GCNNGC 2 cut(s) 78, 137
CfoI GCGC 1 cut(s) 18
Cfr10I RCCGGY 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 121
CseI GACGC 1 cut(s) 107
CviJI RGCY 2 cut(s) 80, 175
CviKI_1 RGCY 2 cut(s) 80, 175
DpnI GATC 2 cut(s) 5, 162
DpnII GATC 2 cut(s) 3, 160
Eco105I TACGTA 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 121
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoT22I ATGCAT 1 cut(s) 112
Esp3I CGTCTC 1 cut(s) 90
FaiI YATR 2 cut(s) 143, 149
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 31, 74, 133
Fsp4HI GCNGC 3 cut(s) 31, 74, 133
FspBI CTAG 1 cut(s) 104
GlaI GCGC 1 cut(s) 17
GluI GCNGC 3 cut(s) 31, 74, 133
HaeIII GGCC 1 cut(s) 80
HapII CCGG 1 cut(s) 136
HgaI GACGC 1 cut(s) 107
HhaI GCGC 1 cut(s) 18
Hin1I GRCGYC 1 cut(s) 207
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 1 cut(s) 151
HpaII CCGG 1 cut(s) 136
HphI GGTGA 1 cut(s) 16
Hpy188I TCNGA 2 cut(s) 44, 205
Hpy188III TCNNGA 2 cut(s) 65, 169
HpyAV CCTTC 1 cut(s) 86
HpyCH4IV ACGT 2 cut(s) 59, 156
HpyCH4V TGCA 2 cut(s) 76, 110
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 132
HpySE526I ACGT 2 cut(s) 59, 156
Hsp92I GRCGYC 1 cut(s) 207
HspAI GCGC 1 cut(s) 16
KroI GCCGGC 1 cut(s) 135
KroNI GCCGGC 1 cut(s) 137
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 160
LpnPI CCDG 6 cut(s) 24, 40, 62, 83, 104, 149
Lsp1109I GCAGC 2 cut(s) 60, 119
LweI GCATC 1 cut(s) 97
MaeI CTAG 1 cut(s) 104
MaeII ACGT 2 cut(s) 59, 156
MalI GATC 2 cut(s) 5, 162
MboI GATC 2 cut(s) 3, 160
MlyI GAGTC 1 cut(s) 145
Mph1103I ATGCAT 1 cut(s) 112
MroNI GCCGGC 1 cut(s) 135
MslI CAYNNNNRTG 1 cut(s) 11
MspI CCGG 1 cut(s) 136
MwoI GCNNNNNNNGC 2 cut(s) 107, 132
NaeI GCCGGC 1 cut(s) 137
NdeII GATC 2 cut(s) 3, 160
NgoMIV GCCGGC 1 cut(s) 135
NsiI ATGCAT 1 cut(s) 112
PaqCI CACCTGC 1 cut(s) 35
PdiI GCCGGC 1 cut(s) 137
PkrI GCNGC 3 cut(s) 32, 75, 134
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
Ppu21I YACGTR 1 cut(s) 60
PspPI GGNCC 1 cut(s) 121
PstI CTGCAG 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 11
SatI GCNGC 3 cut(s) 31, 74, 133
Sau3AI GATC 2 cut(s) 3, 160
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 1 cut(s) 145
SetI ASST 6 cut(s) 29, 39, 62, 72, 159, 177
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 74
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 11
SnaBI TACGTA 1 cut(s) 60
SsiI CCGC 2 cut(s) 30, 124
SspMI CTAG 1 cut(s) 104
TaiI ACGT 2 cut(s) 62, 159
TaqI TCGA 2 cut(s) 66, 168
TauI GCSGC 1 cut(s) 33
TscAI CASTG 1 cut(s) 18
TseI GCWGC 2 cut(s) 73, 132
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 74
XspI CTAG 1 cut(s) 104
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.