MD10G1096900.v1.1

Protein unc-50 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014148
Physical Location & Seq
Forward (+)
15450062 .. 15467548
17487 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1096900.v1.1.491

Sequence Viewer

Length: 765 bp
ATGTTGCCGACAACGTCGAGAGGGCGGTCGTCGTCCTCTTCATCTCGGACGAACCCCATGCCCATTCAGTACCTTCGGAGAATAATCAAGTGGCAACAAATGGATATTGAATATACTTTTTGGCAAATGCTTCACCTATGCACCTCACCGAAAGTTGTCTATCAGCACACTAAATATCACAAGCAAACTAAGAATCAATGGGCACGCGATGATCCTGCTTTTGTTGTAATCTGCAGCGTTCTACTTGCAGTTGCAACTCTGGCTTATTGTGCCGCGTACGACCATAGTGCTGCACATGCTGTTTTTGTAGTTATTTCCGTTTTGTTTTTTCATTTTTTGTTCATCGGGATGCTTCTGGCTACATTTTGCTGGTTTTTGACTAATTCTTACCTCCGTGAAGAGGCTCCGAATAGCCATGTTGTTGAGCAGCATGTTGAATGGCTGTACGCGTTTGATGTGCACTGCAACTCTTTCTTCCCGATGTTTGTTCTGCTTTATGTGATCCATTATTTTCTATCACCAATTTTGGTAGCTCATGGCTTCCTTCCTGTATTGCTATCAAATTTGATATTCATGGTGGCCACTTCATACTATCATTATCTCAACTTTTTAGGTTATGATGTGCTGCCCTTTCTGGAGAGGACCACTTTCTTCCTGTATCCAATCAGTGTTGTCATTGTCCTCTCTCCGATCTTGATTTTGAGTGGCTTCAGTCCTTCAAGATACTTTATGAACATGTACTTTAGTCAACGAGTATGGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UNC-50

Protein Analysis

255

Amino Acids

29.77

Weight (kDa)

8.55

Isoelectric Point (pI)

52.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UNC-50 PF05216 24 - 248 3.9e-84 UNC-50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15240
fragaria_vesca FvH4_2g05170
malus_domestica MD05G1089700.v1.1 MD10G1096900.v1.1
prunus_persica Prupe.8G135000_v2.0.a1 Prupe.8G135000_v2.0.a1
pyrus_communis pycom05g08880 pycom10g08300
rosa_chinensis RchiOBHm_Chr6g0255081
rosa_laevigata RLG00000014853
rosa_multiflora Rmu_sc0004590.1_g000003
rosa_roxburghii Rroxscaffold_7G00210190
rosa_rugosa Rorug05G0557000 Rorug05G0557100
rosa_samantha Rh6BG066600 Rh6CG066200
rosa_wichuraiana Rw6G006490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 207, 275, 449
AciI CCGC 2 cut(s) 25, 273
AclWI GGATC 2 cut(s) 206, 496
AcoI YGGCCR 1 cut(s) 579
AcsI RAATTY 1 cut(s) 562
AcuI CTGAAG 1 cut(s) 694
AfaI GTAC 4 cut(s) 71, 278, 446, 740
AfiI CCNNNNNNNGG 1 cut(s) 400
AflIII ACRYGT 2 cut(s) 447, 735
AgsI TTSAA 3 cut(s) 110, 437, 720
AluBI AGCT 1 cut(s) 533
AluI AGCT 1 cut(s) 533
Alw21I GWGCWC 1 cut(s) 462
Alw44I GTGCAC 1 cut(s) 458
AlwI GGATC 2 cut(s) 206, 496
AoxI GGCC 1 cut(s) 579
ApaLI GTGCAC 1 cut(s) 458
ApeKI GCWGC 4 cut(s) 234, 290, 427, 625
ApoI RAATTY 1 cut(s) 562
AspS9I GGNCC 1 cut(s) 642
AsuHPI GGTGA 3 cut(s) 125, 138, 510
AvaII GGWCC 1 cut(s) 642
BaeGI GKGCMC 2 cut(s) 205, 462
BalI TGGCCA 1 cut(s) 581
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 4 cut(s) 246, 277, 439, 612
BcgI CGANNNNNNTGC 6 cut(s) 40, 74, 197, 231, 269, 303
BciVI GTATCC 1 cut(s) 669
BfmI CTRYAG 1 cut(s) 232
BfuI GTATCC 1 cut(s) 669
BisI GCNGC 5 cut(s) 235, 273, 291, 428, 626
BlsI GCNGC 5 cut(s) 236, 274, 292, 429, 627
Bme18I GGWCC 1 cut(s) 642
BmgT120I GGNCC 1 cut(s) 642
BmiI GGNNCC 1 cut(s) 405
BmsI GCATC 1 cut(s) 339
BpmI CTGGAG 1 cut(s) 656
Bsc4I CCNNNNNNNGG 1 cut(s) 400
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 1 cut(s) 400
BseSI GKGCMC 2 cut(s) 205, 462
BseXI GCAGC 4 cut(s) 246, 277, 439, 612
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 3 cut(s) 207, 275, 449
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 581
BsiEI CGRYCG 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 462
BsiWI CGTACG 1 cut(s) 276
BslI CCNNNNNNNGG 1 cut(s) 400
BsnI GGCC 1 cut(s) 581
Bsp1286I GDGCHC 2 cut(s) 205, 462
Bsp143I GATC 3 cut(s) 211, 501, 690
BspACI CCGC 2 cut(s) 25, 273
BspANI GGCC 1 cut(s) 581
BspFNI CGCG 3 cut(s) 207, 275, 449
BspLI GGNNCC 1 cut(s) 405
BspMAI CTGCAG 1 cut(s) 236
BspPI GGATC 2 cut(s) 206, 496
BssMI GATC 3 cut(s) 211, 501, 690
Bst6I CTCTTC 2 cut(s) 43, 393
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 354
BstFNI CGCG 3 cut(s) 207, 275, 449
BstKTI GATC 3 cut(s) 214, 504, 693
BstMBI GATC 3 cut(s) 211, 501, 690
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 3 cut(s) 260, 269, 296
BstNSI RCATGY 3 cut(s) 299, 434, 739
BstSFI CTRYAG 1 cut(s) 232
BstSLI GKGCMC 2 cut(s) 205, 462
BstUI CGCG 3 cut(s) 207, 275, 449
BstV1I GCAGC 4 cut(s) 246, 277, 439, 612
BsuI GTATCC 1 cut(s) 669
BsuRI GGCC 1 cut(s) 581
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 354
BtsI GCAGTG 1 cut(s) 460
BtsIMutI CAGTG 2 cut(s) 460, 673
Cac8I GCNNGC 1 cut(s) 205
Cfr13I GGNCC 1 cut(s) 642
Csp6I GTAC 4 cut(s) 70, 277, 445, 739
CviAII CATG 7 cut(s) 58, 296, 416, 431, 536, 574, 736
CviJI RGCY 9 cut(s) 263, 359, 404, 414, 442, 533, 540, 581, 708
CviKI_1 RGCY 9 cut(s) 263, 359, 404, 414, 442, 533, 540, 581, 708
CviQI GTAC 4 cut(s) 70, 277, 445, 739
DdeI CTNAG 1 cut(s) 189
DpnI GATC 3 cut(s) 213, 503, 692
DpnII GATC 3 cut(s) 211, 501, 690
EaeI YGGCCR 1 cut(s) 579
Eam1104I CTCTTC 2 cut(s) 43, 393
EarI CTCTTC 2 cut(s) 43, 393
Eco47I GGWCC 1 cut(s) 642
Eco57I CTGAAG 1 cut(s) 694
FaeI CATG 7 cut(s) 61, 299, 419, 434, 539, 577, 739
FatI CATG 7 cut(s) 57, 295, 415, 430, 535, 573, 735
Fnu4HI GCNGC 5 cut(s) 235, 273, 291, 428, 626
FokI GGATG 1 cut(s) 361
Fsp4HI GCNGC 5 cut(s) 235, 273, 291, 428, 626
GluI GCNGC 5 cut(s) 235, 273, 291, 428, 626
GsuI CTGGAG 1 cut(s) 656
HaeIII GGCC 1 cut(s) 581
Hin1II CATG 7 cut(s) 61, 299, 419, 434, 539, 577, 739
HincII GTYRAC 1 cut(s) 749
HindII GTYRAC 1 cut(s) 749
HinfI GANTC 1 cut(s) 193
HphI GGTGA 3 cut(s) 125, 138, 510
Hpy166II GTNNAC 2 cut(s) 460, 749
Hpy188I TCNGA 4 cut(s) 48, 78, 408, 690
Hpy188III TCNNGA 6 cut(s) 18, 346, 478, 635, 694, 720
Hpy8I GTNNAC 2 cut(s) 460, 749
Hpy99I CGWCG 2 cut(s) 19, 34
HpyAV CCTTC 3 cut(s) 83, 554, 726
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 7 cut(s) 141, 234, 248, 254, 293, 460, 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 269, 296
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 7 cut(s) 61, 299, 419, 434, 539, 577, 739
Kzo9I GATC 3 cut(s) 211, 501, 690
LmnI GCTCC 1 cut(s) 409
LpnPI CCDG 7 cut(s) 228, 245, 341, 355, 561, 620, 668
Lsp1109I GCAGC 4 cut(s) 246, 277, 439, 612
LweI GCATC 1 cut(s) 339
MaeII ACGT 1 cut(s) 14
MalI GATC 3 cut(s) 213, 503, 692
MboI GATC 3 cut(s) 211, 501, 690
MboII GAAGA 4 cut(s) 30, 410, 466, 643
MhlI GDGCHC 2 cut(s) 205, 462
MlsI TGGCCA 1 cut(s) 581
MluCI AATT 3 cut(s) 382, 522, 562
MluI ACGCGT 1 cut(s) 447
MluNI TGGCCA 1 cut(s) 581
MnlI CCTC 7 cut(s) 14, 46, 154, 394, 401, 633, 692
Mox20I TGGCCA 1 cut(s) 581
MscI TGGCCA 1 cut(s) 581
MseI TTAA 1 cut(s) 763
MslI CAYNNNNRTG 1 cut(s) 347
Msp20I TGGCCA 1 cut(s) 581
MvnI CGCG 3 cut(s) 207, 275, 449
MwoI GCNNNNNNNGC 3 cut(s) 260, 269, 296
NdeII GATC 3 cut(s) 211, 501, 690
NlaIII CATG 7 cut(s) 61, 299, 419, 434, 539, 577, 739
NlaIV GGNNCC 1 cut(s) 405
NspI RCATGY 3 cut(s) 299, 434, 739
PciI ACATGT 1 cut(s) 735
PfeI GAWTC 1 cut(s) 193
Pfl23II CGTACG 1 cut(s) 276
PflFI GACNNNGTC 1 cut(s) 13
PkrI GCNGC 5 cut(s) 236, 274, 292, 429, 627
PscI ACATGT 1 cut(s) 735
PspLI CGTACG 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 1 cut(s) 642
PstI CTGCAG 1 cut(s) 236
PsyI GACNNNGTC 1 cut(s) 13
RsaI GTAC 4 cut(s) 71, 278, 446, 740
RsaNI GTAC 4 cut(s) 70, 277, 445, 739
RseI CAYNNNNRTG 1 cut(s) 347
SaqAI TTAA 1 cut(s) 763
SatI GCNGC 5 cut(s) 235, 273, 291, 428, 626
Sau3AI GATC 3 cut(s) 211, 501, 690
Sau96I GGNCC 1 cut(s) 642
SduI GDGCHC 2 cut(s) 205, 462
SetI ASST 7 cut(s) 17, 75, 138, 146, 393, 535, 616
SfaNI GCATC 1 cut(s) 339
SfcI CTRYAG 1 cut(s) 232
SinI GGWCC 1 cut(s) 642
SmiMI CAYNNNNRTG 1 cut(s) 347
Sse9I AATT 3 cut(s) 382, 522, 562
SsiI CCGC 2 cut(s) 25, 273
TaiI ACGT 1 cut(s) 17
TaqI TCGA 1 cut(s) 17
TasI AATT 3 cut(s) 382, 522, 562
TatI WGTACW 1 cut(s) 738
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 1 cut(s) 763
Tru9I TTAA 1 cut(s) 763
TscAI CASTG 2 cut(s) 467, 673
TseI GCWGC 4 cut(s) 234, 290, 427, 625
TspDTI ATGAA 6 cut(s) 30, 320, 331, 562, 576, 746
TspGWI ACGGA 2 cut(s) 307, 383
TspRI CASTG 2 cut(s) 467, 673
Tth111I GACNNNGTC 1 cut(s) 13
VneI GTGCAC 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 642
XapI RAATTY 1 cut(s) 562
XceI RCATGY 3 cut(s) 299, 434, 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.