Rroxscaffold_7G00210190

Protein unc-50 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0014148
Physical Location & Seq
Forward (+)
60435154 .. 60440429
5276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00210190.1

Sequence Viewer

Length: 762 bp
ATGTTGCCGACGACTTCGAGAGGTCGGTCGTCGTCCTCTACATCTCGGACGACCAATCCCCTCTTCATTCAGTACCTCCGCCGCATAATCAAGTGGCAGCAAATGGATATTGAGTATACATTTTGGCAAATGCTTCATCTATGCACCTCACCAAAAGTTGTCTATCAGCACACTAAGTATCACAAGCAAACTAAGAACCAATGGGCACGTGATGATCCTGCTTTTGTTGTGATCTGCAGCCTTCTACTTGCAGTTGCAACCCTGGCTTATTGTGCTGCGTATGATCATAGTGCTGCACATGCCGTTTTTGTAGTTATTTCAGTTTTGCTTTTCCATTTTTTGTTCATTGGGATGCTTCTGGCTACGTTTTGCTGGTTCCTGACTAATTCTTACCTTCGGGAAGAGGCTCCAAATAGTCATGTTGTTGAGCAGCGTGTTGAATGGCTCTACGCATTTGATGTGCACTGCAACTCTTTCTTCCCGATGTTTGTTATGCTCTACGTGCTCCATTATTTTCTGTCACCTCTTTTGGTAGCTCATGGTTTTATCCCTGTATTGCTATCAAATTTGCTATTCATGGTGGCTTCTTCATACTATCATTATCTCAACTTCTTAGGTTATGATGTACTGCCATTCCTGGAAAGGACCACTTTCTTCCTGTACCCAATTAGTATTGTCATAGTCCTCTCTCCTATCTTGATTTTGAGTGGCTTCAATCCTTCAAGATATTTCATGAACATGTATTTCAGTCATTGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UNC-50

Protein Analysis

253

Amino Acids

29.72

Weight (kDa)

8.55

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UNC-50 PF05216 25 - 249 3.2e-85 UNC-50 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15240
fragaria_vesca FvH4_2g05170
malus_domestica MD05G1089700.v1.1 MD10G1096900.v1.1
prunus_persica Prupe.8G135000_v2.0.a1 Prupe.8G135000_v2.0.a1
pyrus_communis pycom05g08880 pycom10g08300
rosa_chinensis RchiOBHm_Chr6g0255081
rosa_laevigata RLG00000014853
rosa_multiflora Rmu_sc0004590.1_g000003
rosa_roxburghii Rroxscaffold_7G00210190
rosa_rugosa Rorug05G0557000 Rorug05G0557100
rosa_samantha Rh6BG066600 Rh6CG066200
rosa_wichuraiana Rw6G006490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 116
AciI CCGC 2 cut(s) 79, 82
AclWI GGATC 1 cut(s) 209
AcsI RAATTY 1 cut(s) 565
AcvI CACGTG 1 cut(s) 209
AfaI GTAC 3 cut(s) 74, 627, 662
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 3 cut(s) 440, 715, 723
AjnI CCWGG 2 cut(s) 261, 636
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
AluBI AGCT 1 cut(s) 536
AluI AGCT 1 cut(s) 536
Alw21I GWGCWC 2 cut(s) 465, 507
Alw44I GTGCAC 1 cut(s) 461
AlwI GGATC 1 cut(s) 209
ApaLI GTGCAC 1 cut(s) 461
ApeKI GCWGC 5 cut(s) 97, 237, 275, 293, 430
ApoI RAATTY 1 cut(s) 565
AspS9I GGNCC 1 cut(s) 645
AsuHPI GGTGA 2 cut(s) 141, 513
AvaII GGWCC 1 cut(s) 645
BaeGI GKGCMC 2 cut(s) 208, 465
BbrPI CACGTG 1 cut(s) 209
Bbv12I GWGCWC 2 cut(s) 465, 507
BbvI GCAGC 5 cut(s) 109, 249, 262, 280, 442
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 2 cut(s) 263, 638
BclI TGATCA 1 cut(s) 283
BfmI CTRYAG 1 cut(s) 235
BisI GCNGC 6 cut(s) 82, 98, 238, 276, 294, 431
BlsI GCNGC 6 cut(s) 83, 99, 239, 277, 295, 432
Bme1390I CCNGG 2 cut(s) 263, 638
Bme18I GGWCC 1 cut(s) 645
BmgT120I GGNCC 1 cut(s) 645
BmiI GGNNCC 2 cut(s) 377, 408
BmrFI CCNGG 2 cut(s) 263, 638
BmsI GCATC 1 cut(s) 342
BsaAI YACGTR 2 cut(s) 209, 502
BsaJI CCNNGG 1 cut(s) 261
BseBI CCWGG 2 cut(s) 263, 638
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 1 cut(s) 357
BseSI GKGCMC 2 cut(s) 208, 465
BseXI GCAGC 5 cut(s) 109, 249, 262, 280, 442
BsgI GTGCAG 1 cut(s) 279
Bsh1285I CGRYCG 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 29
BsiHKAI GWGCWC 2 cut(s) 465, 507
Bsp1286I GDGCHC 3 cut(s) 208, 465, 507
Bsp143I GATC 3 cut(s) 214, 231, 283
BspACI CCGC 2 cut(s) 79, 82
BspHI TCATGA 1 cut(s) 732
BspLI GGNNCC 2 cut(s) 377, 408
BspMAI CTGCAG 1 cut(s) 239
BspPI GGATC 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 3 cut(s) 214, 231, 283
BssNAI GTATAC 1 cut(s) 117
Bst1107I GTATAC 1 cut(s) 117
Bst2UI CCWGG 2 cut(s) 263, 638
Bst6I CTCTTC 2 cut(s) 68, 396
BstBAI YACGTR 2 cut(s) 209, 502
BstDEI CTNAG 3 cut(s) 174, 192, 613
BstF5I GGATG 1 cut(s) 357
BstKTI GATC 3 cut(s) 217, 234, 286
BstMBI GATC 3 cut(s) 214, 231, 283
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 4 cut(s) 263, 272, 299, 502
BstNI CCWGG 2 cut(s) 263, 638
BstNSI RCATGY 2 cut(s) 302, 742
BstSCI CCNGG 2 cut(s) 261, 636
BstSFI CTRYAG 1 cut(s) 235
BstSLI GKGCMC 2 cut(s) 208, 465
BstV1I GCAGC 5 cut(s) 109, 249, 262, 280, 442
BstZ17I GTATAC 1 cut(s) 117
BtsCI GGATG 1 cut(s) 357
BtsI GCAGTG 1 cut(s) 463
BtsIMutI CAGTG 1 cut(s) 463
CciI TCATGA 1 cut(s) 732
Cfr13I GGNCC 1 cut(s) 645
Csp6I GTAC 3 cut(s) 73, 626, 661
CviAII CATG 6 cut(s) 299, 419, 539, 577, 733, 739
CviJI RGCY 8 cut(s) 240, 266, 362, 407, 445, 536, 584, 711
CviKI_1 RGCY 8 cut(s) 240, 266, 362, 407, 445, 536, 584, 711
CviQI GTAC 3 cut(s) 73, 626, 661
DdeI CTNAG 3 cut(s) 174, 192, 613
DpnI GATC 3 cut(s) 216, 233, 285
DpnII GATC 3 cut(s) 214, 231, 283
Eam1104I CTCTTC 2 cut(s) 68, 396
EarI CTCTTC 2 cut(s) 68, 396
EciI GGCGGA 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 645
Eco72I CACGTG 1 cut(s) 209
EcoRII CCWGG 2 cut(s) 261, 636
FaeI CATG 6 cut(s) 302, 422, 542, 580, 736, 742
FalI AAGNNNNNCTT 2 cut(s) 634, 666
FatI CATG 6 cut(s) 298, 418, 538, 576, 732, 738
FbaI TGATCA 1 cut(s) 283
FblI GTMKAC 1 cut(s) 116
Fnu4HI GCNGC 6 cut(s) 82, 98, 238, 276, 294, 431
FokI GGATG 1 cut(s) 364
Fsp4HI GCNGC 6 cut(s) 82, 98, 238, 276, 294, 431
GluI GCNGC 6 cut(s) 82, 98, 238, 276, 294, 431
Hin1II CATG 6 cut(s) 302, 422, 542, 580, 736, 742
HphI GGTGA 2 cut(s) 141, 513
Hpy166II GTNNAC 2 cut(s) 117, 463
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 7 cut(s) 18, 379, 398, 481, 697, 723, 733
Hpy8I GTNNAC 2 cut(s) 117, 463
Hpy99I CGWCG 2 cut(s) 13, 34
HpyAV CCTTC 3 cut(s) 251, 404, 729
HpyCH4IV ACGT 3 cut(s) 208, 365, 501
HpyCH4V TGCA 7 cut(s) 144, 237, 251, 257, 296, 463, 468
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 272, 299, 502
HpyF3I CTNAG 3 cut(s) 174, 192, 613
HpySE526I ACGT 3 cut(s) 208, 365, 501
Hsp92II CATG 6 cut(s) 302, 422, 542, 580, 736, 742
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 3 cut(s) 214, 231, 283
LmnI GCTCC 2 cut(s) 412, 510
Lsp1109I GCAGC 5 cut(s) 109, 249, 262, 280, 442
LweI GCATC 1 cut(s) 342
MaeII ACGT 3 cut(s) 208, 365, 501
MaeIII GTNAC 1 cut(s) 519
MalI GATC 3 cut(s) 216, 233, 285
MboI GATC 3 cut(s) 214, 231, 283
MboII GAAGA 5 cut(s) 55, 413, 469, 579, 646
MhlI GDGCHC 3 cut(s) 208, 465, 507
MluCI AATT 3 cut(s) 385, 565, 666
MnlI CCTC 8 cut(s) 14, 46, 71, 86, 157, 397, 534, 695
MslI CAYNNNNRTG 2 cut(s) 350, 737
MspR9I CCNGG 2 cut(s) 263, 638
MvaI CCWGG 2 cut(s) 263, 638
MwoI GCNNNNNNNGC 4 cut(s) 263, 272, 299, 502
NdeII GATC 3 cut(s) 214, 231, 283
NlaIII CATG 6 cut(s) 302, 422, 542, 580, 736, 742
NlaIV GGNNCC 2 cut(s) 377, 408
NmuCI GTSAC 1 cut(s) 519
NspI RCATGY 2 cut(s) 302, 742
PagI TCATGA 1 cut(s) 732
PciI ACATGT 1 cut(s) 738
PfoI TCCNGGA 1 cut(s) 636
PkrI GCNGC 6 cut(s) 83, 99, 239, 277, 295, 432
PmaCI CACGTG 1 cut(s) 209
PmlI CACGTG 1 cut(s) 209
Ppu21I YACGTR 2 cut(s) 209, 502
PscI ACATGT 1 cut(s) 738
Psp6I CCWGG 2 cut(s) 261, 636
PspCI CACGTG 1 cut(s) 209
PspGI CCWGG 2 cut(s) 261, 636
PspN4I GGNNCC 2 cut(s) 377, 408
PspPI GGNCC 1 cut(s) 645
PstI CTGCAG 1 cut(s) 239
RsaI GTAC 3 cut(s) 74, 627, 662
RsaNI GTAC 3 cut(s) 73, 626, 661
RseI CAYNNNNRTG 2 cut(s) 350, 737
SatI GCNGC 6 cut(s) 82, 98, 238, 276, 294, 431
Sau3AI GATC 3 cut(s) 214, 231, 283
Sau96I GGNCC 1 cut(s) 645
ScrFI CCNGG 2 cut(s) 263, 638
SduI GDGCHC 3 cut(s) 208, 465, 507
SfaNI GCATC 1 cut(s) 342
SfcI CTRYAG 1 cut(s) 235
SinI GGWCC 1 cut(s) 645
SmiMI CAYNNNNRTG 2 cut(s) 350, 737
Sse9I AATT 3 cut(s) 385, 565, 666
SsiI CCGC 2 cut(s) 79, 82
StyD4I CCNGG 2 cut(s) 261, 636
TaiI ACGT 3 cut(s) 211, 368, 504
TaqI TCGA 1 cut(s) 17
TaqII GACCGA 1 cut(s) 15
TasI AATT 3 cut(s) 385, 565, 666
TatI WGTACW 1 cut(s) 625
TauI GCSGC 1 cut(s) 84
TscAI CASTG 1 cut(s) 470
TseFI GTSAC 1 cut(s) 519
TseI GCWGC 5 cut(s) 97, 237, 275, 293, 430
Tsp45I GTSAC 1 cut(s) 519
TspDTI ATGAA 7 cut(s) 55, 125, 334, 565, 579, 721, 749
TspRI CASTG 1 cut(s) 470
VneI GTGCAC 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 645
XapI RAATTY 1 cut(s) 565
XceI RCATGY 2 cut(s) 302, 742
XmiI GTMKAC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.