Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007715

Physical Location & Seq

Genomic Coordinates Reverse (-)

23183506 .. 23188057

Gene Structure 4552 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1145500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2403 bp

ATGAGAGGCCTGGATTGCATATGGCGCTTCCCCAACGCCCACTCTCGCCGCCATTTATGTCCCCTGATCTCTCCTCCTCCGTCTTCACCGCCGCCGAGAAAAACCGTCCTCCTATTCTCTCCCACATCGAACACCCAAACCAGAACAATCGACACCAAAAATGCCAGTGCTGGGTACACGTCAAACACAAGAACTAGTAAACCCATAAAAAACGGCAGAGCTCTCACTCCCAGACCTCCTCTCTCCCTCCCTCAAACCCAACAAACCCAGCAACAAAACGATGACAATGACAACGCAGAAGAACAAGAAGAAGAAGAGGAAGATGAAGATGAAAGCAGAGCACAAGGCCGTGGCCGCGGGAACTGGACTATAGTGCTTTCGTCGAGCTTGGTGGGTCTGCTCACCGGAATCGGCGTCGTTCTCTTCAATTACTCTGTAAATGAGATCCGTGATGTGTTCTGGGGTGGGATGCCTCAACGGGGAGCTTCATGGCTGAGAGAGGAGTACGTGGGAGATACGTGGAAGCAGGTGATTCTGGTTCCGGCTTCTGGGGGTTTGATCGTTGCCATATTAAACTTTCTTCGAGATGCTCTCGAGGATGGTTCACAAGATAGCAATGGCAATGAGCAAGGTGGAGAAAAAGGGGAGAATTTATTAGTGAAAATGGGAATGCCTGTTTCGGTTTTTGATGGTCTCAAGGCTGCATCGCGTCCTTTTCTCAAGGCAATTGCTGCTTGTGTTACACTTGGGACTGGGAATTCGTTAGGTCCCGAAGGTCCTAGCGTTGAGATTGGAACTTCTATAGCCAAGGGTGTCGGTGCTGTGTTTGATAAAAGTTCGCAAAGAAGGCTTTCTCTTGTGGCTGCTGGATCAGCTGCTGGAATTGCTTCGGGGTTTAATGCTGCTGTTTCGGGTTGTTTCTTTGCTGTAGAGTCAGTCTTATGGCCATCACCATCTGATTCCTCATCTATTTCGCTTGTGAATACCACTTCTATGGTCATACTTAGTGCTGTGATAGCTTCTGTAGTTTCAGAAGTTGGTCTAGGTTCTGAACCAGCCTTTAAGGTCCCATACTATGACTTCCGCTCACCTACTGAACTTCCGCTATATCTATTGCTGGGTGTCCTATGTGGCTTGGTTTCGTTAACCTTATCAAAGTGCACATCGTACATGTTGGTATTTGTTGACAATCTTCACAAGACCATTGGCCTACCAAAATTTTTGTTCCCTATCATGGGTGGGTTAGCAGTCGGACTTATGGCATTGGCATATCCTGAAATTCTTTATCGGGGTTTCGAGAATGTTGACATTTTGCTGGAGTCTCAACCACTTGAGAAATGCCTCTCAGCTGATCTGTTACTTCAGCTGGTAGTGGTCAAGATAGGTGCAACCTCATTGTCTCGAGCCTCTGGATTAGTTGGAGGCTTCTATGCTCCATCACTTTTTATTGGTGCAGCAACAGGAATGGCATACGGGAAATTCATCAGTTCTGCAGTTGCTCAGTCCAGTCCTATGCTTCATCTTTCCGTCTTGGAAGTAGCATCACCACAAGCATACGGCCTGGTTGGAATGGCTGCTACTCTGGCAGGAGTCTGTCAAGTACCTCTGACGTCGGTTCTGCTGCTTTTTGAATTGACACAAGATTATCGTATTGTACTACCGCTACTTGGAGCTGTAGGGTTATCTTCGTGGATTACATCTGGACAGACAAGGAGAAAAGATATCTTGAAGACTAAGAAACTAAAACAGGGAAAGTCTAGTGGCAATCAACAACCTGAAGTACCTTCCCCTAGTGCAAGTGGGCTTTCTTCTAGTAATGCTTTCACATCTAATGCAAGCGACCTTTGTGAATTAGAGAGTTCACTCTGTATAGATGGTTCTGATATTAATACTGAGAACCTAGAGAAGAGGATATCTGTATCCGAGGCCATGAGGACAAGATATGTGACCATTTTAATGGACACGTTGCTTACAGAAGCAGTGACTCTCATGCTTGCAGAAAAGCAGCCCTGTGCCATGATTGTCGATGATGATCATATTCTAATTGGTTTACTGACACTTAGAGACATTCAAGAGTTCAGCAATAAATATGCAAAATCAAGACGACAAAGACCCAAGGAGCTTATAGTATCTGAAATGTGTTCTTCAGACGGGGAAGTATGCCGAGTACCATGGACGGCTACGCCTGCTATGACTCTACTTTCAGCTCAGAACATTATGAATAAGTATGGAATGAATCAAATTCCAGTTGTTACACAGCATGTTCAAGATCACAGAGGGCACTTAGTTGGCCTTCTAGACAGAGAATGTATCACTCTCACGTGCAGCTTTAGCAACCAGAGAATCCCTTTGCAAGTGACCAATACCGGATGCACTGATTTGTTGATAAAACAGAATACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0016020

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

801

Amino Acids

85.96

Weight (kDa)

5.81

Isoelectric Point (pI)

45.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	239 - 566	1.3e-71	Voltage gated chloride channel
CBS	PF00571	640 - 692	2.6e-07	CBS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007715)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35440 AT4G35440
fragaria_vesca	FvH4_2g17250 FvH4_2g17250 FvH4_2g17250 FvH4_2g17250 FvH4_2g17250
malus_domestica	MD10G1145500.v1.1
prunus_persica	Prupe.8G183800_v2.0.a1 Prupe.8G183800_v2.0.a1 Prupe.8G183800_v2.0.a1
pyrus_communis	pycom10g12560
rosa_chinensis	RchiOBHm_Chr6g0281741
rosa_laevigata	RLG00000012939
rosa_multiflora	Rmu_sc0008470.1_g000002 Rmu_ssc0000126.1_g000061 Rmu_ssc0000126.1_g000062
rosa_roxburghii	Rroxscaffold_7G00186860
rosa_rugosa	Rorug06G0143500
rosa_samantha	Rh6AG255900 Rh6BG259000 Rh6CG257900 Rh6DG250000
rosa_wichuraiana	Rw6G022180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	517
AatII	GACGTC	1 cut(s)	1613
Acc36I	ACCTGC	1 cut(s)	517
AccBSI	CCGCTC	1 cut(s)	1086
AccII	CGCG	2 cut(s)	357, 709
AciI	CCGC	8 cut(s)	49, 89, 92, 355, 357, 1084, 1103, 1661
AclWI	GGATC	2 cut(s)	439, 877
AcoI	YGGCCR	2 cut(s)	352, 944
AcsI	RAATTY	6 cut(s)	649, 757, 1217, 1278, 1478, 2241
AcuI	CTGAAG	3 cut(s)	1346, 1797, 2130
AcvI	CACGTG	1 cut(s)	2322
AcyI	GRCGYC	2 cut(s)	414, 1610
AfaI	GTAC	7 cut(s)	176, 506, 1169, 1602, 1656, 1782, 2169
AfiI	CCNNNNNNNGG	6 cut(s)	171, 479, 548, 1234, 1235, 1667
AflIII	ACRYGT	3 cut(s)	177, 1170, 1962
AgsI	TTSAA	5 cut(s)	427, 1631, 1729, 2072, 2267
AhlI	ACTAGT	1 cut(s)	194
AjiI	CACGTC	1 cut(s)	180
AjnI	CCWGG	2 cut(s)	9, 1560
Alw21I	GWGCWC	3 cut(s)	223, 343, 1163
Alw26I	GTCTC	4 cut(s)	698, 1326, 1404, 2058
Alw44I	GTGCAC	1 cut(s)	1159
AlwI	GGATC	2 cut(s)	439, 877
AlwNI	CAGNNNCTG	1 cut(s)	878
Ama87I	CYCGRG	2 cut(s)	593, 1401
AoxI	GGCC	8 cut(s)	7, 346, 352, 944, 1207, 1558, 1926, 2290
ApaLI	GTGCAC	1 cut(s)	1159
ApoI	RAATTY	6 cut(s)	649, 757, 1217, 1278, 1478, 2241
ArsI	GACNNNNNNTTYG	2 cut(s)	742, 774
AseI	ATTAAT	1 cut(s)	1887
Asp700I	GAANNNNTTC	2 cut(s)	850, 886
AspLEI	GCGC	1 cut(s)	27
AspS9I	GGNCC	3 cut(s)	767, 776, 1066
AsuHPI	GGTGA	6 cut(s)	78, 394, 541, 942, 1080, 1536
AvaI	CYCGRG	2 cut(s)	593, 1401
AvaII	GGWCC	3 cut(s)	767, 776, 1066
BaeGI	GKGCMC	2 cut(s)	1163, 2283
BalI	TGGCCA	1 cut(s)	946
BanII	GRGCYC	1 cut(s)	223
BbrPI	CACGTG	1 cut(s)	2322
BbsI	GAAGAC	2 cut(s)	75, 1736
Bbv12I	GWGCWC	3 cut(s)	223, 343, 1163
BccI	CCATC	6 cut(s)	593, 683, 955, 961, 1444, 1868
BceAI	ACGGC	4 cut(s)	229, 333, 1573, 2193
BciT130I	CCWGG	2 cut(s)	11, 1562
BciVI	GTATCC	1 cut(s)	1930
BclI	TGATCA	1 cut(s)	2032
BcoDI	GTCTC	4 cut(s)	698, 1326, 1404, 2058
BcuI	ACTAGT	1 cut(s)	194
BfaI	CTAG	8 cut(s)	195, 780, 1043, 1758, 1791, 1812, 1901, 2297
BfmI	CTRYAG	6 cut(s)	369, 801, 927, 1023, 1491, 1674
BfoI	RGCGCY	1 cut(s)	28
BfuAI	ACCTGC	1 cut(s)	517
BfuI	GTATCC	1 cut(s)	1930
Bme1390I	CCNGG	2 cut(s)	11, 1562
Bme18I	GGWCC	3 cut(s)	767, 776, 1066
BmeT110I	CYCGRG	2 cut(s)	593, 1401
BmgBI	CACGTC	1 cut(s)	180
BmgT120I	GGNCC	3 cut(s)	767, 776, 1066
BmiI	GGNNCC	3 cut(s)	540, 769, 1068
BmrFI	CCNGG	2 cut(s)	11, 1562
BmrI	ACTGGG	1 cut(s)	762
BmsI	GCATC	5 cut(s)	459, 577, 713, 1550, 2360
BmuI	ACTGGG	1 cut(s)	762
BpiI	GAAGAC	2 cut(s)	75, 1736
BplI	GAGNNNNNCTC	2 cut(s)	576, 608
BpmI	CTGGAG	1 cut(s)	1337
BpuEI	CTTGAG	3 cut(s)	680, 704, 1352
BsaAI	YACGTR	3 cut(s)	508, 519, 2322
BsaBI	GATNNNNATC	1 cut(s)	2031
BsaHI	GRCGYC	2 cut(s)	414, 1610
BsaI	GGTCTC	1 cut(s)	698
BsaJI	CCNNGG	6 cut(s)	349, 355, 807, 1923, 2115, 2171
BsaWI	WCCGGW	2 cut(s)	404, 2366
Bsc4I	CCNNNNNNNGG	6 cut(s)	171, 479, 548, 1234, 1235, 1667
Bse1I	ACTGG	5 cut(s)	165, 368, 757, 1506, 2246
Bse3DI	GCAATG	2 cut(s)	622, 628
Bse8I	GATNNNNATC	1 cut(s)	2031
BseBI	CCWGG	2 cut(s)	11, 1562
BseDI	CCNNGG	6 cut(s)	349, 355, 807, 1923, 2115, 2171
BseGI	GGATG	3 cut(s)	474, 604, 2375
BseJI	GATNNNNATC	1 cut(s)	2031
BseLI	CCNNNNNNNGG	6 cut(s)	171, 479, 548, 1234, 1235, 1667
BseMI	GCAATG	2 cut(s)	622, 628
BseMII	CTCAG	5 cut(s)	485, 1359, 1514, 1884, 2222
BseNI	ACTGG	5 cut(s)	165, 368, 757, 1506, 2246
BseRI	GAGGAG	4 cut(s)	63, 66, 228, 515
BseSI	GKGCMC	2 cut(s)	1163, 2283
BseYI	CCCAGC	3 cut(s)	170, 267, 1117
BsgI	GTGCAG	2 cut(s)	1473, 2344
Bsh1236I	CGCG	2 cut(s)	357, 709
BshFI	GGCC	8 cut(s)	9, 348, 354, 946, 1209, 1560, 1928, 2292
BsiHKAI	GWGCWC	3 cut(s)	223, 343, 1163
BsiHKCI	CYCGRG	2 cut(s)	593, 1401
BsiSI	CCGG	3 cut(s)	405, 542, 2367
BslFI	GGGAC	4 cut(s)	45, 753, 763, 1052
BslI	CCNNNNNNNGG	6 cut(s)	171, 479, 548, 1234, 1235, 1667
BsmAI	GTCTC	4 cut(s)	698, 1326, 1404, 2058
BsmFI	GGGAC	4 cut(s)	45, 753, 763, 1052
BsmI	GAATGC	1 cut(s)	675
BsnI	GGCC	8 cut(s)	9, 348, 354, 946, 1209, 1560, 1928, 2292
Bso31I	GGTCTC	1 cut(s)	698
BsoBI	CYCGRG	2 cut(s)	593, 1401
Bsp1286I	GDGCHC	4 cut(s)	223, 343, 1163, 2283
Bsp143I	GATC	7 cut(s)	66, 444, 558, 869, 1351, 2032, 2269
Bsp19I	CCATGG	1 cut(s)	2171
BspACI	CCGC	8 cut(s)	49, 89, 92, 355, 357, 1084, 1103, 1661
BspANI	GGCC	8 cut(s)	9, 348, 354, 946, 1209, 1560, 1928, 2292
BspCNI	CTCAG	5 cut(s)	486, 1358, 1513, 1885, 2221
BspFNI	CGCG	2 cut(s)	357, 709
BspLI	GGNNCC	3 cut(s)	540, 769, 1068
BspMAI	CTGCAG	1 cut(s)	1495
BspMI	ACCTGC	1 cut(s)	517
BspPI	GGATC	2 cut(s)	439, 877
BspTNI	GGTCTC	1 cut(s)	698
BsrBI	CCGCTC	1 cut(s)	1086
BsrDI	GCAATG	2 cut(s)	622, 628
BsrI	ACTGG	5 cut(s)	165, 368, 757, 1506, 2246
BssECI	CCNNGG	6 cut(s)	349, 355, 807, 1923, 2115, 2171
BssMI	GATC	7 cut(s)	66, 444, 558, 869, 1351, 2032, 2269
BssNI	GRCGYC	2 cut(s)	414, 1610
BssT1I	CCWWGG	3 cut(s)	807, 2115, 2171
Bst2UI	CCWGG	2 cut(s)	11, 1562
Bst4CI	ACNGT	1 cut(s)	106
Bst6I	CTCTTC	3 cut(s)	309, 428, 1901
BstACI	GRCGYC	2 cut(s)	414, 1610
BstAPI	GCANNNNNTGC	1 cut(s)	731
BstBAI	YACGTR	3 cut(s)	508, 519, 2322
BstC8I	GCNNGC	3 cut(s)	1837, 1995, 2187
BstDEI	CTNAG	9 cut(s)	494, 1004, 1345, 1500, 1734, 1893, 2060, 2208, 2284
BstDSI	CCRYGG	3 cut(s)	349, 355, 2171
BstF5I	GGATG	3 cut(s)	474, 604, 2375
BstFNI	CGCG	2 cut(s)	357, 709
BstH2I	RGCGCY	1 cut(s)	28
BstHHI	GCGC	1 cut(s)	27
BstKTI	GATC	7 cut(s)	69, 447, 561, 872, 1354, 2035, 2272
BstMAI	GTCTC	4 cut(s)	698, 1326, 1404, 2058
BstMBI	GATC	7 cut(s)	66, 444, 558, 869, 1351, 2032, 2269
BstNI	CCWGG	2 cut(s)	11, 1562
BstNSI	RCATGY	2 cut(s)	1174, 2264
BstSCI	CCNGG	2 cut(s)	9, 1560
BstSFI	CTRYAG	6 cut(s)	369, 801, 927, 1023, 1491, 1674
BstSLI	GKGCMC	2 cut(s)	1163, 2283
BstUI	CGCG	2 cut(s)	357, 709
BstV2I	GAAGAC	2 cut(s)	75, 1736
BstX2I	RGATCY	1 cut(s)	444
BstXI	CCANNNNNNTGG	2 cut(s)	994, 1957
BstYI	RGATCY	1 cut(s)	444
BsuI	GTATCC	1 cut(s)	1930
BsuRI	GGCC	8 cut(s)	9, 348, 354, 946, 1209, 1560, 1928, 2292
BtgI	CCRYGG	3 cut(s)	349, 355, 2171
BtgZI	GCGATG	1 cut(s)	690
BtrI	CACGTC	1 cut(s)	180
BtsCI	GGATG	3 cut(s)	474, 604, 2375
BtsI	GCAGTG	1 cut(s)	1986
BtsIMutI	CAGTG	3 cut(s)	172, 1986, 2373
BveI	ACCTGC	1 cut(s)	517
Cac8I	GCNNGC	3 cut(s)	1837, 1995, 2187
CaiI	CAGNNNCTG	1 cut(s)	878
CfoI	GCGC	1 cut(s)	27
Cfr13I	GGNCC	3 cut(s)	767, 776, 1066
Cfr42I	CCGCGG	1 cut(s)	358
CseI	GACGC	2 cut(s)	403, 698
Csp6I	GTAC	7 cut(s)	175, 505, 1168, 1601, 1655, 1781, 2168
CviAII	CATG	8 cut(s)	489, 1171, 1234, 1930, 1990, 2017, 2172, 2261
CviQI	GTAC	7 cut(s)	175, 505, 1168, 1601, 1655, 1781, 2168
DdeI	CTNAG	9 cut(s)	494, 1004, 1345, 1500, 1734, 1893, 2060, 2208, 2284
DpnI	GATC	7 cut(s)	68, 446, 560, 871, 1353, 2034, 2271
DpnII	GATC	7 cut(s)	66, 444, 558, 869, 1351, 2032, 2269
EaeI	YGGCCR	2 cut(s)	352, 944
Eam1104I	CTCTTC	3 cut(s)	309, 428, 1901
EarI	CTCTTC	3 cut(s)	309, 428, 1901
Ecl136II	GAGCTC	1 cut(s)	221
Eco130I	CCWWGG	3 cut(s)	807, 2115, 2171
Eco147I	AGGCCT	1 cut(s)	9
Eco24I	GRGCYC	1 cut(s)	223
Eco31I	GGTCTC	1 cut(s)	698
Eco32I	GATATC	2 cut(s)	1723, 1914
Eco47I	GGWCC	3 cut(s)	767, 776, 1066
Eco53kI	GAGCTC	1 cut(s)	221
Eco57I	CTGAAG	3 cut(s)	1346, 1797, 2130
Eco72I	CACGTG	1 cut(s)	2322
Eco88I	CYCGRG	2 cut(s)	593, 1401
EcoICRI	GAGCTC	1 cut(s)	221
EcoO109I	RGGNCCY	3 cut(s)	767, 776, 1066
EcoRI	GAATTC	1 cut(s)	757
EcoRII	CCWGG	2 cut(s)	9, 1560
EcoRV	GATATC	2 cut(s)	1723, 1914
EcoT14I	CCWWGG	3 cut(s)	807, 2115, 2171
EcoT38I	GRGCYC	1 cut(s)	223
ErhI	CCWWGG	3 cut(s)	807, 2115, 2171
FaeI	CATG	8 cut(s)	492, 1174, 1237, 1933, 1993, 2020, 2175, 2264
FaqI	GGGAC	4 cut(s)	45, 753, 763, 1052
FatI	CATG	8 cut(s)	488, 1170, 1233, 1929, 1989, 2016, 2171, 2260
FauI	CCCGC	1 cut(s)	350
FauNDI	CATATG	1 cut(s)	20
FbaI	TGATCA	1 cut(s)	2032
FokI	GGATG	3 cut(s)	481, 611, 2382
FriOI	GRGCYC	1 cut(s)	223
FspBI	CTAG	8 cut(s)	195, 780, 1043, 1758, 1791, 1812, 1901, 2297
GlaI	GCGC	1 cut(s)	26
GsaI	CCCAGC	3 cut(s)	174, 271, 1121
GsuI	CTGGAG	1 cut(s)	1337
HaeII	RGCGCY	1 cut(s)	28
HaeIII	GGCC	8 cut(s)	9, 348, 354, 946, 1209, 1560, 1928, 2292
HapII	CCGG	3 cut(s)	405, 542, 2367
HgaI	GACGC	2 cut(s)	403, 698
HhaI	GCGC	1 cut(s)	27
Hin1I	GRCGYC	2 cut(s)	414, 1610
Hin1II	CATG	8 cut(s)	492, 1174, 1237, 1933, 1993, 2020, 2175, 2264
Hin6I	GCGC	1 cut(s)	25
HinP1I	GCGC	1 cut(s)	25
HincII	GTYRAC	3 cut(s)	1146, 1186, 1306
HindII	GTYRAC	3 cut(s)	1146, 1186, 1306
HpaI	GTTAAC	1 cut(s)	1146
HpaII	CCGG	3 cut(s)	405, 542, 2367
HphI	GGTGA	6 cut(s)	78, 394, 541, 942, 1080, 1536
Hpy166II	GTNNAC	9 cut(s)	177, 200, 605, 1146, 1161, 1186, 1306, 1862, 2051
Hpy8I	GTNNAC	9 cut(s)	177, 200, 605, 1146, 1161, 1186, 1306, 1862, 2051
Hpy99I	CGWCG	3 cut(s)	385, 419, 1615
HpyAV	CCTTC	4 cut(s)	767, 840, 1794, 2303
HpyCH4III	ACNGT	1 cut(s)	106
HpyCH4IV	ACGT	6 cut(s)	179, 507, 518, 1610, 1964, 2321
HpyF3I	CTNAG	9 cut(s)	494, 1004, 1345, 1500, 1734, 1893, 2060, 2208, 2284
HpySE526I	ACGT	6 cut(s)	179, 507, 518, 1610, 1964, 2321
Hsp92I	GRCGYC	2 cut(s)	414, 1610
Hsp92II	CATG	8 cut(s)	492, 1174, 1237, 1933, 1993, 2020, 2175, 2264
HspAI	GCGC	1 cut(s)	25
Ksp22I	TGATCA	1 cut(s)	2032
KspAI	GTTAAC	1 cut(s)	1146
KspI	CCGCGG	1 cut(s)	358
Kzo9I	GATC	7 cut(s)	66, 444, 558, 869, 1351, 2032, 2269
LmnI	GCTCC	4 cut(s)	482, 1438, 1670, 2119
LweI	GCATC	5 cut(s)	459, 577, 713, 1550, 2360
MaeI	CTAG	8 cut(s)	195, 780, 1043, 1758, 1791, 1812, 1901, 2297
MaeII	ACGT	6 cut(s)	179, 507, 518, 1610, 1964, 2321
MaeIII	GTNAC	6 cut(s)	739, 1356, 1945, 1981, 2251, 2356
MalI	GATC	7 cut(s)	68, 446, 560, 871, 1353, 2034, 2271
MbiI	CCGCTC	1 cut(s)	1086
MboI	GATC	7 cut(s)	66, 444, 558, 869, 1351, 2032, 2269
MfeI	CAATTG	1 cut(s)	726
MflI	RGATCY	1 cut(s)	444
MhlI	GDGCHC	4 cut(s)	223, 343, 1163, 2283
MlsI	TGGCCA	1 cut(s)	946
MluNI	TGGCCA	1 cut(s)	946
MlyI	GAGTC	5 cut(s)	941, 1328, 1599, 1978, 2188
MmeI	TCCRAC	3 cut(s)	1231, 1399, 1546
Mox20I	TGGCCA	1 cut(s)	946
MroXI	GAANNNNTTC	2 cut(s)	850, 886
MscI	TGGCCA	1 cut(s)	946
MseI	TTAA	6 cut(s)	572, 897, 1062, 1145, 1887, 1955
MslI	CAYNNNNRTG	2 cut(s)	992, 1955
Msp20I	TGGCCA	1 cut(s)	946
MspA1I	CMGCKG	4 cut(s)	357, 875, 1349, 1366
MspI	CCGG	3 cut(s)	405, 542, 2367
MspR9I	CCNGG	2 cut(s)	11, 1562
MunI	CAATTG	1 cut(s)	726
Mva1269I	GAATGC	1 cut(s)	675
MvaI	CCWGG	2 cut(s)	11, 1562
MvnI	CGCG	2 cut(s)	357, 709
NcoI	CCATGG	1 cut(s)	2171
NdeI	CATATG	1 cut(s)	20
NdeII	GATC	7 cut(s)	66, 444, 558, 869, 1351, 2032, 2269
NlaIII	CATG	8 cut(s)	492, 1174, 1237, 1933, 1993, 2020, 2175, 2264
NlaIV	GGNNCC	3 cut(s)	540, 769, 1068
NmeAIII	GCCGAG	2 cut(s)	120, 2189
NmuCI	GTSAC	3 cut(s)	1945, 1981, 2356
NspI	RCATGY	2 cut(s)	1174, 2264
PaeR7I	CTCGAG	2 cut(s)	593, 1401
PaqCI	CACCTGC	1 cut(s)	517
PceI	AGGCCT	1 cut(s)	9
PciI	ACATGT	1 cut(s)	1170
PctI	GAATGC	1 cut(s)	675
PdmI	GAANNNNTTC	2 cut(s)	850, 886
PfeI	GAWTC	5 cut(s)	408, 532, 959, 2236, 2343
PleI	GAGTC	5 cut(s)	940, 1327, 1598, 1978, 2188
PmaCI	CACGTG	1 cut(s)	2322
PmlI	CACGTG	1 cut(s)	2322
PpsI	GAGTC	5 cut(s)	940, 1327, 1598, 1978, 2188
Ppu21I	YACGTR	3 cut(s)	508, 519, 2322
PpuMI	RGGWCCY	3 cut(s)	767, 776, 1066
PscI	ACATGT	1 cut(s)	1170
PshBI	ATTAAT	1 cut(s)	1887
Psp124BI	GAGCTC	1 cut(s)	223
Psp5II	RGGWCCY	3 cut(s)	767, 776, 1066
Psp6I	CCWGG	2 cut(s)	9, 1560
PspCI	CACGTG	1 cut(s)	2322
PspFI	CCCAGC	3 cut(s)	170, 267, 1117
PspGI	CCWGG	2 cut(s)	9, 1560
PspN4I	GGNNCC	3 cut(s)	540, 769, 1068
PspPI	GGNCC	3 cut(s)	767, 776, 1066
PspPPI	RGGWCCY	3 cut(s)	767, 776, 1066
PsrI	GAACNNNNNNTAC	2 cut(s)	1861, 1893
PstI	CTGCAG	1 cut(s)	1495
PstNI	CAGNNNCTG	1 cut(s)	878
PsuI	RGATCY	1 cut(s)	444
PvuII	CAGCTG	3 cut(s)	875, 1349, 1366
RsaI	GTAC	7 cut(s)	176, 506, 1169, 1602, 1656, 1782, 2169
RsaNI	GTAC	7 cut(s)	175, 505, 1168, 1601, 1655, 1781, 2168
RseI	CAYNNNNRTG	2 cut(s)	992, 1955
SacI	GAGCTC	1 cut(s)	223
SacII	CCGCGG	1 cut(s)	358
SaqAI	TTAA	6 cut(s)	572, 897, 1062, 1145, 1887, 1955
Sau3AI	GATC	7 cut(s)	66, 444, 558, 869, 1351, 2032, 2269
Sau96I	GGNCC	3 cut(s)	767, 776, 1066
SchI	GAGTC	5 cut(s)	941, 1328, 1599, 1978, 2188
ScrFI	CCNGG	2 cut(s)	11, 1562
SduI	GDGCHC	4 cut(s)	223, 343, 1163, 2283
SfaNI	GCATC	5 cut(s)	459, 577, 713, 1550, 2360
SfcI	CTRYAG	6 cut(s)	369, 801, 927, 1023, 1491, 1674
Sfr274I	CTCGAG	2 cut(s)	593, 1401
Sfr303I	CCGCGG	1 cut(s)	358
SgrBI	CCGCGG	1 cut(s)	358
SinI	GGWCC	3 cut(s)	767, 776, 1066
SlaI	CTCGAG	2 cut(s)	593, 1401
SmiMI	CAYNNNNRTG	2 cut(s)	992, 1955
SmlI	CTYRAG	5 cut(s)	593, 695, 719, 1331, 1401
SmoI	CTYRAG	5 cut(s)	593, 695, 719, 1331, 1401
SpeI	ACTAGT	1 cut(s)	194
SseBI	AGGCCT	1 cut(s)	9
SsiI	CCGC	8 cut(s)	49, 89, 92, 355, 357, 1084, 1103, 1661
SspMI	CTAG	8 cut(s)	195, 780, 1043, 1758, 1791, 1812, 1901, 2297
SstI	GAGCTC	1 cut(s)	223
StuI	AGGCCT	1 cut(s)	9
StyD4I	CCNGG	2 cut(s)	9, 1560
StyI	CCWWGG	3 cut(s)	807, 2115, 2171
TaaI	ACNGT	1 cut(s)	106
TaiI	ACGT	6 cut(s)	182, 510, 521, 1613, 1967, 2324
TaqI	TCGA	8 cut(s)	128, 150, 383, 583, 594, 1296, 1402, 2025
TatI	WGTACW	1 cut(s)	1654
TauI	GCSGC	3 cut(s)	51, 94, 357
TfiI	GAWTC	5 cut(s)	408, 532, 959, 2236, 2343
Tru1I	TTAA	6 cut(s)	572, 897, 1062, 1145, 1887, 1955
Tru9I	TTAA	6 cut(s)	572, 897, 1062, 1145, 1887, 1955
TscAI	CASTG	3 cut(s)	172, 1986, 2380
TseFI	GTSAC	3 cut(s)	1945, 1981, 2356
Tsp45I	GTSAC	3 cut(s)	1945, 1981, 2356
TspDTI	ATGAA	7 cut(s)	339, 345, 477, 1471, 1508, 2234, 2249
TspGWI	ACGGA	3 cut(s)	69, 437, 1516
TspRI	CASTG	3 cut(s)	172, 1986, 2380
VneI	GTGCAC	1 cut(s)	1159
VpaK11BI	GGWCC	3 cut(s)	767, 776, 1066
VspI	ATTAAT	1 cut(s)	1887
XapI	RAATTY	6 cut(s)	649, 757, 1217, 1278, 1478, 2241
XbaI	TCTAGA	1 cut(s)	2296
XceI	RCATGY	2 cut(s)	1174, 2264
XhoI	CTCGAG	2 cut(s)	593, 1401
XmnI	GAANNNNTTC	2 cut(s)	850, 886
XspI	CTAG	8 cut(s)	195, 780, 1043, 1758, 1791, 1812, 1901, 2297
ZraI	GACGTC	1 cut(s)	1611

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.