Rmu_sc0008470.1_g000002

Chloride channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008470.1
OG0007715
Physical Location & Seq
Forward (+)
3980 .. 4938
959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008470.1_g000002.1.cds

Sequence Viewer

Length: 840 bp
atgggcggtctccactgtaaatggcggtttcccaacacccgctttaaccgtcatttgtgtcgtctaccctcctctcctccatttccaccattctcaacaaacacttcactactattctctccattatcacaacccaaaatcactaccaacgacagaactgctggtgttggatttacggcaacaaacactagaagtagaagtagaggtctcagacctctgttctctctccctcaaaatcaacaaacccagcaagtacaagaagacaacgaaccaaacaatgaagaagaagatgaggacatttcccaaggccgtggcaatgggaactggacaatagtactatcatccagcttggttggtctcctcaccggcctcggtgtcgttctcttcaactactcagtgcatgagataagagaggtgttttgggatgggatacctcaaagaggagcttcatggctgagagaggagcccattactgatatgtggcagcgagtgattctggttccggcttccgggggtttgattgtggccattttgaatgtgcttagaggtgcattggatgatgaacaaggagaagaaaatgaagaagaagagaatgtattggtgaaaatgggaatgcccgtttcggtttttgatagcttgaaggctgcgtcacgtccatttcttaaggctgttgctgcttgtgtcactctgggaactgggaattctttaggtccggaagggcctagtgttgagattgggacctccattgccagaggtgtaagttctgtgtttgatagaagttcgcagagaaagctctctcttgtggctgctggatcagctgctggaattgcttctggttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS Voltage_CLC

Protein Analysis

279

Amino Acids

30.17

Weight (kDa)

5.52

Isoelectric Point (pI)

52.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 64
AccIII TCCGGA 1 cut(s) 712
AciI CCGC 3 cut(s) 6, 25, 40
AclWI GGATC 1 cut(s) 820
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 1 cut(s) 700
AfaI GTAC 2 cut(s) 255, 336
AfiI CCNNNNNNNGG 2 cut(s) 440, 509
AflII CTTAAG 1 cut(s) 662
AgsI TTSAA 3 cut(s) 388, 535, 640
AjiI CACGTC 1 cut(s) 653
AluBI AGCT 5 cut(s) 348, 446, 636, 793, 818
AluI AGCT 5 cut(s) 348, 446, 636, 793, 818
Alw26I GTCTC 3 cut(s) 14, 212, 362
AlwI GGATC 1 cut(s) 820
AlwNI CAGNNNCTG 1 cut(s) 821
Aor13HI TCCGGA 1 cut(s) 712
AoxI GGCC 4 cut(s) 307, 367, 525, 719
ApeKI GCWGC 5 cut(s) 484, 644, 674, 806, 818
ApoI RAATTY 1 cut(s) 700
Asp700I GAANNNNTTC 1 cut(s) 829
AspS9I GGNCC 3 cut(s) 710, 719, 738
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 2 cut(s) 355, 613
AvaII GGWCC 2 cut(s) 710, 738
BalI TGGCCA 1 cut(s) 527
BanII GRGCYC 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 267
BbvI GCAGC 5 cut(s) 496, 631, 661, 793, 805
BccI CCATC 1 cut(s) 419
BceAI ACGGC 2 cut(s) 192, 294
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciVI GTATCC 1 cut(s) 423
BcnI CCSGG 1 cut(s) 511
BcoDI GTCTC 3 cut(s) 14, 212, 362
BfaI CTAG 2 cut(s) 189, 723
BfrI CTTAAG 1 cut(s) 662
BfuI GTATCC 1 cut(s) 423
BisI GCNGC 5 cut(s) 485, 645, 675, 807, 819
BlsI GCNGC 5 cut(s) 486, 646, 676, 808, 820
BmcAI AGTACT 1 cut(s) 336
Bme1390I CCNGG 1 cut(s) 511
Bme18I GGWCC 2 cut(s) 710, 738
BmgBI CACGTC 1 cut(s) 653
BmgT120I GGNCC 3 cut(s) 710, 719, 738
BmiI GGNNCC 3 cut(s) 465, 501, 739
BmrFI CCNGG 1 cut(s) 511
BmrI ACTGGG 1 cut(s) 705
BmuI ACTGGG 1 cut(s) 705
BpiI GAAGAC 1 cut(s) 267
BpuMI CCSGG 1 cut(s) 511
BsaI GGTCTC 3 cut(s) 14, 212, 362
BsaJI CCNNGG 4 cut(s) 304, 310, 370, 510
BsaWI WCCGGW 1 cut(s) 712
Bsc4I CCNNNNNNNGG 2 cut(s) 440, 509
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 2 cut(s) 329, 700
Bse3DI GCAATG 2 cut(s) 322, 744
BseAI TCCGGA 1 cut(s) 712
BseDI CCNNGG 4 cut(s) 304, 310, 370, 510
BseGI GGATG 3 cut(s) 341, 430, 562
BseLI CCNNNNNNNGG 2 cut(s) 440, 509
BseMI GCAATG 2 cut(s) 322, 744
BseMII CTCAG 3 cut(s) 223, 408, 446
BseNI ACTGG 2 cut(s) 329, 700
BseRI GAGGAG 5 cut(s) 61, 66, 350, 456, 476
BseXI GCAGC 5 cut(s) 496, 631, 661, 793, 805
BseYI CCCAGC 1 cut(s) 246
BshFI GGCC 4 cut(s) 309, 369, 527, 721
BsiSI CCGG 4 cut(s) 366, 503, 510, 713
BslFI GGGAC 1 cut(s) 751
BslI CCNNNNNNNGG 2 cut(s) 440, 509
BsmAI GTCTC 3 cut(s) 14, 212, 362
BsmFI GGGAC 1 cut(s) 751
BsmI GAATGC 1 cut(s) 618
BsnI GGCC 4 cut(s) 309, 369, 527, 721
Bso31I GGTCTC 3 cut(s) 14, 212, 362
Bsp1286I GDGCHC 1 cut(s) 468
Bsp13I TCCGGA 1 cut(s) 712
Bsp143I GATC 1 cut(s) 812
BspACI CCGC 3 cut(s) 6, 25, 40
BspANI GGCC 4 cut(s) 309, 369, 527, 721
BspCNI CTCAG 3 cut(s) 222, 407, 447
BspEI TCCGGA 1 cut(s) 712
BspLI GGNNCC 3 cut(s) 465, 501, 739
BspPI GGATC 1 cut(s) 820
BspTI CTTAAG 1 cut(s) 662
BspTNI GGTCTC 3 cut(s) 14, 212, 362
BsrDI GCAATG 2 cut(s) 322, 744
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 2 cut(s) 329, 700
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 4 cut(s) 304, 310, 370, 510
BssMI GATC 1 cut(s) 812
BssT1I CCWWGG 1 cut(s) 304
Bst4CI ACNGT 2 cut(s) 17, 50
Bst6I CTCTTC 2 cut(s) 389, 582
BstAFI CTTAAG 1 cut(s) 662
BstDEI CTNAG 4 cut(s) 209, 394, 455, 542
BstDSI CCRYGG 1 cut(s) 310
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 3 cut(s) 341, 430, 562
BstKTI GATC 1 cut(s) 815
BstMAI GTCTC 3 cut(s) 14, 212, 362
BstMBI GATC 1 cut(s) 812
BstMWI GCNNNNNNNGC 4 cut(s) 674, 790, 815, 827
BstSCI CCNGG 1 cut(s) 509
BstV1I GCAGC 5 cut(s) 496, 631, 661, 793, 805
BstV2I GAAGAC 1 cut(s) 267
BstXI CCANNNNNNTGG 1 cut(s) 311
BsuI GTATCC 1 cut(s) 423
BsuRI GGCC 4 cut(s) 309, 369, 527, 721
BtgI CCRYGG 1 cut(s) 310
BtrI CACGTC 1 cut(s) 653
BtsCI GGATG 3 cut(s) 341, 430, 562
BtsIMutI CAGTG 2 cut(s) 13, 402
CaiI CAGNNNCTG 1 cut(s) 821
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 3 cut(s) 710, 719, 738
CseI GACGC 1 cut(s) 636
Csp6I GTAC 2 cut(s) 254, 335
CviAII CATG 2 cut(s) 401, 450
CviQI GTAC 2 cut(s) 254, 335
DdeI CTNAG 4 cut(s) 209, 394, 455, 542
DpnI GATC 1 cut(s) 814
DpnII GATC 1 cut(s) 812
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 2 cut(s) 389, 582
EarI CTCTTC 2 cut(s) 389, 582
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 468
Eco31I GGTCTC 3 cut(s) 14, 212, 362
Eco47I GGWCC 2 cut(s) 710, 738
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoO109I RGGNCCY 2 cut(s) 719, 738
EcoRI GAATTC 1 cut(s) 700
EcoT14I CCWWGG 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 2 cut(s) 404, 453
FaiI YATR 3 cut(s) 402, 451, 479
FalI AAGNNNNNCTT 2 cut(s) 430, 462
FaqI GGGAC 1 cut(s) 751
FatI CATG 2 cut(s) 400, 449
FauI CCCGC 1 cut(s) 47
FblI GTMKAC 1 cut(s) 64
Fnu4HI GCNGC 5 cut(s) 485, 645, 675, 807, 819
FokI GGATG 3 cut(s) 328, 437, 569
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 5 cut(s) 485, 645, 675, 807, 819
FspBI CTAG 2 cut(s) 189, 723
GluI GCNGC 5 cut(s) 485, 645, 675, 807, 819
GsaI CCCAGC 1 cut(s) 250
HaeIII GGCC 4 cut(s) 309, 369, 527, 721
HapII CCGG 4 cut(s) 366, 503, 510, 713
HgaI GACGC 1 cut(s) 636
Hin1II CATG 2 cut(s) 404, 453
HinfI GANTC 1 cut(s) 493
HpaII CCGG 4 cut(s) 366, 503, 510, 713
HphI GGTGA 2 cut(s) 355, 613
Hpy166II GTNNAC 1 cut(s) 65
Hpy188I TCNGA 1 cut(s) 212
Hpy188III TCNNGA 1 cut(s) 713
Hpy8I GTNNAC 1 cut(s) 65
HpyAV CCTTC 2 cut(s) 634, 710
HpyCH4III ACNGT 2 cut(s) 17, 50
HpyCH4IV ACGT 1 cut(s) 652
HpyCH4V TGCA 2 cut(s) 400, 551
HpyF10VI GCNNNNNNNGC 4 cut(s) 674, 790, 815, 827
HpyF3I CTNAG 4 cut(s) 209, 394, 455, 542
HpySE526I ACGT 1 cut(s) 652
Hsp92II CATG 2 cut(s) 404, 453
Kpn2I TCCGGA 1 cut(s) 712
Kzo9I GATC 1 cut(s) 812
LmnI GCTCC 2 cut(s) 443, 463
Lsp1109I GCAGC 5 cut(s) 496, 631, 661, 793, 805
MaeI CTAG 2 cut(s) 189, 723
MaeII ACGT 1 cut(s) 652
MaeIII GTNAC 2 cut(s) 648, 682
MalI GATC 1 cut(s) 814
MboI GATC 1 cut(s) 812
MboII GAAGA 9 cut(s) 272, 293, 296, 299, 376, 584, 593, 596, 599
MhlI GDGCHC 1 cut(s) 468
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 2 cut(s) 700, 825
MluNI TGGCCA 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 148
Mox20I TGGCCA 1 cut(s) 527
MroI TCCGGA 1 cut(s) 712
MroXI GAANNNNTTC 1 cut(s) 829
MscI TGGCCA 1 cut(s) 527
MseI TTAA 2 cut(s) 45, 663
Msp20I TGGCCA 1 cut(s) 527
MspA1I CMGCKG 1 cut(s) 818
MspCI CTTAAG 1 cut(s) 662
MspI CCGG 4 cut(s) 366, 503, 510, 713
MspR9I CCNGG 1 cut(s) 511
Mva1269I GAATGC 1 cut(s) 618
MwoI GCNNNNNNNGC 4 cut(s) 674, 790, 815, 827
NciI CCSGG 1 cut(s) 511
NdeII GATC 1 cut(s) 812
NlaIII CATG 2 cut(s) 404, 453
NlaIV GGNNCC 3 cut(s) 465, 501, 739
NmuCI GTSAC 2 cut(s) 648, 682
PctI GAATGC 1 cut(s) 618
PdmI GAANNNNTTC 1 cut(s) 829
PfeI GAWTC 1 cut(s) 493
PkrI GCNGC 5 cut(s) 486, 646, 676, 808, 820
PpuMI RGGWCCY 1 cut(s) 738
Psp5II RGGWCCY 1 cut(s) 738
PspFI CCCAGC 1 cut(s) 246
PspN4I GGNNCC 3 cut(s) 465, 501, 739
PspPI GGNCC 3 cut(s) 710, 719, 738
PspPPI RGGWCCY 1 cut(s) 738
PstNI CAGNNNCTG 1 cut(s) 821
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 2 cut(s) 255, 336
RsaNI GTAC 2 cut(s) 254, 335
SaqAI TTAA 2 cut(s) 45, 663
SatI GCNGC 5 cut(s) 485, 645, 675, 807, 819
Sau3AI GATC 1 cut(s) 812
Sau96I GGNCC 3 cut(s) 710, 719, 738
ScaI AGTACT 1 cut(s) 336
ScrFI CCNGG 1 cut(s) 511
SduI GDGCHC 1 cut(s) 468
SinI GGWCC 2 cut(s) 710, 738
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
Sse9I AATT 2 cut(s) 700, 825
SsiI CCGC 3 cut(s) 6, 25, 40
SspMI CTAG 2 cut(s) 189, 723
StyD4I CCNGG 1 cut(s) 509
StyI CCWWGG 1 cut(s) 304
TaaI ACNGT 2 cut(s) 17, 50
TaiI ACGT 1 cut(s) 655
TasI AATT 2 cut(s) 700, 825
TatI WGTACW 2 cut(s) 253, 334
TfiI GAWTC 1 cut(s) 493
Tru1I TTAA 2 cut(s) 45, 663
Tru9I TTAA 2 cut(s) 45, 663
TscAI CASTG 2 cut(s) 20, 402
TseFI GTSAC 2 cut(s) 648, 682
TseI GCWGC 5 cut(s) 484, 644, 674, 806, 818
Tsp45I GTSAC 2 cut(s) 648, 682
TspDTI ATGAA 4 cut(s) 294, 438, 576, 594
TspRI CASTG 2 cut(s) 20, 402
Vha464I CTTAAG 1 cut(s) 662
VpaK11BI GGWCC 2 cut(s) 710, 738
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 1 cut(s) 700
XmiI GTMKAC 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 829
XspI CTAG 2 cut(s) 189, 723
ZrmI AGTACT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.