MD10G1193800.v1.1

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016219
Physical Location & Seq
Forward (+)
28977472 .. 28979460
1989 bp
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UTR
Exon/CDS
Intron
MD10G1193800.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGCTTCTTTCAGTATGTTTAAGCTCTCATTTTTCTTGAGCCTTCTCTCCAATCTGAACCTGGGGTTGTGGCCACAAACTCAAAACAGTTTGGTAATATTTCCACCATCATGTAACAGCATAGAGTGTCCTATCTTCGACACAGTCGAGGTCGGGAATGGCTACGAAGTCCGTCGATACAATTCGACAGAGTGGATGTCGACTTCTCCTATTCAAGATATCTCTCTTGTCGAAGCCACCAGAACTGGCTTCTTACAGTTGTTTGACTACATTCAAGGGAAGAACAAGTACGAAGAGAAAATAGAGATGACTGCCCCAGTCCTCAGCGAAGTCTCACCAAGTGAAGGACCCCTCTGTGAGTCCTCATTTGTGGTGAGCTTCTATGTGCCAAAGAAGAACCAAGAAAACCCACCACCAGCAGAAGGCCTTCATCTTCAAACATGGAAACCTAAATATGTTGCGGTGAGGCAATTCAGTGGATTCGTATCTGACTACAACATCGGAGAAGAAGCTGCTGCCTTGCAGGCCAGTCTTGCAGGCACTGTTTGGTCTGCTGCCATTGACAAAAGCCATGCAGCCAATCACACTTCAGCTTATACTGTTGCACAGTACAATTCGCCTTTTGAATTTGATCACAGGGTGAATGAGATATGGATGATATTTGATTTAGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

226

Amino Acids

25.32

Weight (kDa)

4.73

Isoelectric Point (pI)

54.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 41 - 219 2e-53 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17100
fragaria_vesca FvH4_3g16830
malus_domestica MD10G1193800.v1.1
prunus_persica Prupe.4G150500_v2.0.a1
pyrus_communis pycom10g16760
rosa_chinensis RchiOBHm_Chr5g0028041
rosa_laevigata RLG00000033060
rosa_multiflora Rmu_sc0009432.1_g000007
rosa_roxburghii Rroxscaffold_1G00051620
rosa_rugosa Rorug05G0103700
rosa_samantha Rh5AG197300 Rh5BG195500 Rh5CG215700 Rh5DG197100
rosa_wichuraiana Rw5G017990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 200
AciI CCGC 1 cut(s) 461
AcoI YGGCCR 1 cut(s) 71
AcsI RAATTY 1 cut(s) 626
AcuI CTGAAG 1 cut(s) 573
AdeI CACNNNGTG 2 cut(s) 341, 640
AfaI GTAC 2 cut(s) 290, 611
AfiI CCNNNNNNNGG 2 cut(s) 344, 422
AgsI TTSAA 4 cut(s) 215, 275, 437, 626
AjnI CCWGG 1 cut(s) 60
AluBI AGCT 4 cut(s) 25, 378, 512, 593
AluI AGCT 4 cut(s) 25, 378, 512, 593
Alw26I GTCTC 1 cut(s) 337
AlwNI CAGNNNCTG 1 cut(s) 542
AoxI GGCC 3 cut(s) 71, 424, 525
ApeKI GCWGC 4 cut(s) 512, 515, 554, 575
ApoI RAATTY 1 cut(s) 626
Asp700I GAANNNNTTC 1 cut(s) 426
AspS9I GGNCC 1 cut(s) 347
AsuHPI GGTGA 4 cut(s) 327, 385, 475, 652
AvaII GGWCC 1 cut(s) 347
BalI TGGCCA 1 cut(s) 73
BarI GAAGNNNNNNTAC 2 cut(s) 272, 304
BbvCI CCTCAGC 1 cut(s) 323
BbvI GCAGC 4 cut(s) 499, 502, 541, 587
BccI CCATC 1 cut(s) 115
BciT130I CCWGG 1 cut(s) 62
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 1 cut(s) 337
BglI GCCNNNNNGGC 1 cut(s) 524
BisI GCNGC 4 cut(s) 513, 516, 555, 576
BlsI GCNGC 4 cut(s) 514, 517, 556, 577
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 1 cut(s) 347
BmgT120I GGNCC 1 cut(s) 347
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 1 cut(s) 62
BmrI ACTGGG 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 311
Bpu10I CCTNAGC 1 cut(s) 323
BpuEI CTTGAG 1 cut(s) 58
BsaBI GATNNNNATC 1 cut(s) 484
BsaJI CCNNGG 1 cut(s) 61
Bsc4I CCNNNNNNNGG 2 cut(s) 344, 422
Bse1I ACTGG 3 cut(s) 250, 317, 528
Bse8I GATNNNNATC 1 cut(s) 484
BseBI CCWGG 1 cut(s) 62
BseDI CCNNGG 1 cut(s) 61
BseGI GGATG 2 cut(s) 201, 660
BseJI GATNNNNATC 1 cut(s) 484
BseLI CCNNNNNNNGG 2 cut(s) 344, 422
BseMII CTCAG 1 cut(s) 337
BseNI ACTGG 3 cut(s) 250, 317, 528
BseXI GCAGC 4 cut(s) 499, 502, 541, 587
BshFI GGCC 3 cut(s) 73, 426, 527
BslI CCNNNNNNNGG 2 cut(s) 344, 422
BsmAI GTCTC 1 cut(s) 337
BsnI GGCC 3 cut(s) 73, 426, 527
Bsp143I GATC 1 cut(s) 631
BspACI CCGC 1 cut(s) 461
BspANI GGCC 3 cut(s) 73, 426, 527
BspCNI CTCAG 1 cut(s) 336
BspLI GGNNCC 1 cut(s) 349
BsrI ACTGG 3 cut(s) 250, 317, 528
BssECI CCNNGG 1 cut(s) 61
BssMI GATC 1 cut(s) 631
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 6 cut(s) 89, 145, 258, 544, 601, 609
Bst6I CTCTTC 2 cut(s) 288, 666
BstC8I GCNNGC 2 cut(s) 525, 538
BstDEI CTNAG 1 cut(s) 323
BstF5I GGATG 2 cut(s) 201, 660
BstKTI GATC 1 cut(s) 634
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 1 cut(s) 631
BstMWI GCNNNNNNNGC 2 cut(s) 524, 533
BstNI CCWGG 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 60
BstV1I GCAGC 4 cut(s) 499, 502, 541, 587
BsuRI GGCC 3 cut(s) 73, 426, 527
BtsCI GGATG 2 cut(s) 201, 660
BtsIMutI CAGTG 2 cut(s) 481, 540
Cac8I GCNNGC 2 cut(s) 525, 538
CaiI CAGNNNCTG 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 347
Csp6I GTAC 2 cut(s) 289, 610
CviAII CATG 3 cut(s) 111, 441, 572
CviQI GTAC 2 cut(s) 289, 610
DdeI CTNAG 1 cut(s) 323
DpnI GATC 1 cut(s) 633
DpnII GATC 1 cut(s) 631
DraIII CACNNNGTG 2 cut(s) 341, 640
EaeI YGGCCR 1 cut(s) 71
Eam1104I CTCTTC 2 cut(s) 288, 666
EarI CTCTTC 2 cut(s) 288, 666
Eco147I AGGCCT 1 cut(s) 426
Eco32I GATATC 1 cut(s) 220
Eco47I GGWCC 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 573
EcoO109I RGGNCCY 1 cut(s) 347
EcoRII CCWGG 1 cut(s) 60
EcoRV GATATC 1 cut(s) 220
FaeI CATG 3 cut(s) 114, 444, 575
FaiI YATR 9 cut(s) 17, 112, 122, 384, 442, 456, 573, 597, 652
FatI CATG 3 cut(s) 110, 440, 571
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 1 cut(s) 200
Fnu4HI GCNGC 4 cut(s) 513, 516, 555, 576
FokI GGATG 2 cut(s) 208, 667
Fsp4HI GCNGC 4 cut(s) 513, 516, 555, 576
GluI GCNGC 4 cut(s) 513, 516, 555, 576
HaeIII GGCC 3 cut(s) 73, 426, 527
Hin1II CATG 3 cut(s) 114, 444, 575
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HinfI GANTC 2 cut(s) 359, 480
HphI GGTGA 4 cut(s) 327, 385, 475, 652
Hpy166II GTNNAC 1 cut(s) 201
Hpy188I TCNGA 3 cut(s) 57, 490, 503
Hpy188III TCNNGA 3 cut(s) 37, 154, 215
Hpy8I GTNNAC 1 cut(s) 201
Hpy99I CGWCG 1 cut(s) 177
HpyAV CCTTC 4 cut(s) 53, 338, 416, 437
HpyCH4III ACNGT 6 cut(s) 89, 145, 258, 544, 601, 609
HpyCH4V TGCA 4 cut(s) 523, 536, 575, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 533
HpyF3I CTNAG 1 cut(s) 323
Hsp92II CATG 3 cut(s) 114, 444, 575
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 1 cut(s) 631
Lsp1109I GCAGC 4 cut(s) 499, 502, 541, 587
MaeIII GTNAC 1 cut(s) 113
MalI GATC 1 cut(s) 633
MboI GATC 1 cut(s) 631
MboII GAAGA 6 cut(s) 127, 292, 305, 406, 425, 518
MlsI TGGCCA 1 cut(s) 73
MluCI AATT 4 cut(s) 181, 470, 613, 626
MluNI TGGCCA 1 cut(s) 73
MlyI GAGTC 1 cut(s) 368
MnlI CCTC 5 cut(s) 142, 332, 362, 373, 459
Mox20I TGGCCA 1 cut(s) 73
MroXI GAANNNNTTC 1 cut(s) 426
MscI TGGCCA 1 cut(s) 73
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 1 cut(s) 109
Msp20I TGGCCA 1 cut(s) 73
MspR9I CCNGG 1 cut(s) 62
MvaI CCWGG 1 cut(s) 62
MwoI GCNNNNNNNGC 2 cut(s) 524, 533
NdeII GATC 1 cut(s) 631
NlaIII CATG 3 cut(s) 114, 444, 575
NlaIV GGNNCC 1 cut(s) 349
PceI AGGCCT 1 cut(s) 426
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 480
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 4 cut(s) 514, 517, 556, 577
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PpuMI RGGWCCY 1 cut(s) 347
Psp5II RGGWCCY 1 cut(s) 347
Psp6I CCWGG 1 cut(s) 60
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 1 cut(s) 349
PspPI GGNCC 1 cut(s) 347
PspPPI RGGWCCY 1 cut(s) 347
PstNI CAGNNNCTG 1 cut(s) 542
PsyI GACNNNGTC 1 cut(s) 143
RsaI GTAC 2 cut(s) 290, 611
RsaNI GTAC 2 cut(s) 289, 610
RseI CAYNNNNRTG 1 cut(s) 109
SalI GTCGAC 1 cut(s) 199
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 4 cut(s) 513, 516, 555, 576
Sau3AI GATC 1 cut(s) 631
Sau96I GGNCC 1 cut(s) 347
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 1 cut(s) 62
SetI ASST 7 cut(s) 27, 63, 153, 380, 451, 514, 595
SinI GGWCC 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 4 cut(s) 181, 470, 613, 626
SseBI AGGCCT 1 cut(s) 426
SsiI CCGC 1 cut(s) 461
SspI AATATT 1 cut(s) 99
StuI AGGCCT 1 cut(s) 426
StyD4I CCNGG 1 cut(s) 60
TaaI ACNGT 6 cut(s) 89, 145, 258, 544, 601, 609
TaqI TCGA 6 cut(s) 138, 147, 175, 185, 200, 231
TasI AATT 4 cut(s) 181, 470, 613, 626
TatI WGTACW 1 cut(s) 609
TfiI GAWTC 1 cut(s) 480
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 2 cut(s) 481, 547
TseI GCWGC 4 cut(s) 512, 515, 554, 575
TspDTI ATGAA 1 cut(s) 419
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 2 cut(s) 481, 547
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 1 cut(s) 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 58
XmiI GTMKAC 1 cut(s) 200
XmnI GAANNNNTTC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.