Rh5BG195500

Heme-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016219
Physical Location & Seq
Reverse (-)
21666645 .. 21669630
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG195500.1

Sequence Viewer

Length: 675 bp
ATGGCTGCCTCTATGTTTAAGCTCTCAGTTTTCTTGAGCCTGCTCTCCAATCTGAATTTGGGTCTCTGGCCAGAAGCTCAGAAGAGCGTGGCAACATTTCCACCATCATGTAACAGCATAGAGTGCCCGAGCTATGACTCCATTGAGGTTGGCAACGGCTATGAAGTTCGTCGTTACAATTCGAGCGAGTGGATATCAACTGCTCCTATTCAGGACATTTCTTTGGTTGCAGCCACCAGAACTGGCTTCTTACAGTTATTTGATTACATTCAAGGGAAGAACAAGTATGGAGAGAAAATAGAGATGACTGCCCCAGTGCTTAGCGAAGTTCAACCAAGCGATGGACCATTCTGTGAGTCTTCATTTGTGGTGAGCTTCTATGTACCAAAGAAGAACCAAGCAAAACCACCACCAGCAGAAGGCCTCCATGTTCAGAAATGGACATCTACTTATGTGGCTGTGAGACAATTCAGTGGGTTTGTTTCAGACTACGATGTTGGAGTAGAAGCTGCTGCCTTGCAGTCCAGTCTTGCAGGCACTGTTTGGTCCGCTGCCATTGAGAAAAGCCATGGAGCTGATCACACTACAATTTATACTGTTGCACAGTACAACTCTCCATTTGAATTTGATCACAGGGTGAATGAGATCTGGATTTCGTTTAATTTAGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SOUL

Protein Analysis

224

Amino Acids

24.8

Weight (kDa)

4.93

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOUL PF04832 40 - 218 5e-52 SOUL heme-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17100
fragaria_vesca FvH4_3g16830
malus_domestica MD10G1193800.v1.1
prunus_persica Prupe.4G150500_v2.0.a1
pyrus_communis pycom10g16760
rosa_chinensis RchiOBHm_Chr5g0028041
rosa_laevigata RLG00000033060
rosa_multiflora Rmu_sc0009432.1_g000007
rosa_roxburghii Rroxscaffold_1G00051620
rosa_rugosa Rorug05G0103700
rosa_samantha Rh5AG197300 Rh5BG195500 Rh5CG215700 Rh5DG197100
rosa_wichuraiana Rw5G017990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 341
AciI CCGC 1 cut(s) 549
AcoI YGGCCR 1 cut(s) 68
AcsI RAATTY 2 cut(s) 55, 623
AdeI CACNNNGTG 1 cut(s) 637
AfaI GTAC 2 cut(s) 384, 608
AfiI CCNNNNNNNGG 2 cut(s) 341, 419
AgsI TTSAA 3 cut(s) 272, 332, 623
AluBI AGCT 6 cut(s) 22, 77, 132, 375, 509, 575
AluI AGCT 6 cut(s) 22, 77, 132, 375, 509, 575
Alw26I GTCTC 2 cut(s) 68, 457
AlwNI CAGNNNCTG 1 cut(s) 539
Ama87I CYCGRG 1 cut(s) 127
AoxI GGCC 2 cut(s) 68, 421
ApeKI GCWGC 5 cut(s) 5, 230, 509, 512, 551
ApoI RAATTY 2 cut(s) 55, 623
AspS9I GGNCC 2 cut(s) 344, 546
AsuHPI GGTGA 2 cut(s) 382, 649
AvaI CYCGRG 1 cut(s) 127
AvaII GGWCC 2 cut(s) 344, 546
BaeGI GKGCMC 1 cut(s) 128
BalI TGGCCA 1 cut(s) 70
BbsI GAAGAC 1 cut(s) 351
BbvI GCAGC 4 cut(s) 242, 496, 499, 538
BccI CCATC 2 cut(s) 112, 335
BceAI ACGGC 1 cut(s) 172
BclI TGATCA 2 cut(s) 577, 628
BcoDI GTCTC 2 cut(s) 68, 457
BglII AGATCT 1 cut(s) 645
BisI GCNGC 5 cut(s) 6, 231, 510, 513, 552
BlpI GCTNAGC 1 cut(s) 320
BlsI GCNGC 5 cut(s) 7, 232, 511, 514, 553
Bme18I GGWCC 2 cut(s) 344, 546
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 344, 546
BmrI ACTGGG 1 cut(s) 308
BmuI ACTGGG 1 cut(s) 308
BpiI GAAGAC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 320
BpuEI CTTGAG 1 cut(s) 55
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 568
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 419
Bse1I ACTGG 3 cut(s) 247, 314, 525
BseDI CCNNGG 1 cut(s) 568
BseLI CCNNNNNNNGG 2 cut(s) 341, 419
BseMII CTCAG 2 cut(s) 39, 92
BseNI ACTGG 3 cut(s) 247, 314, 525
BseSI GKGCMC 1 cut(s) 128
BseXI GCAGC 4 cut(s) 242, 496, 499, 538
BshFI GGCC 2 cut(s) 70, 423
BsiHKCI CYCGRG 1 cut(s) 127
BslI CCNNNNNNNGG 2 cut(s) 341, 419
BsmAI GTCTC 2 cut(s) 68, 457
BsnI GGCC 2 cut(s) 70, 423
Bso31I GGTCTC 1 cut(s) 68
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 128
Bsp143I GATC 3 cut(s) 577, 628, 645
Bsp1720I GCTNAGC 1 cut(s) 320
Bsp19I CCATGG 1 cut(s) 568
BspACI CCGC 1 cut(s) 549
BspANI GGCC 2 cut(s) 70, 423
BspCNI CTCAG 2 cut(s) 38, 91
BspQI GCTCTTC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 68
BsrI ACTGG 3 cut(s) 247, 314, 525
BssECI CCNNGG 1 cut(s) 568
BssMI GATC 3 cut(s) 577, 628, 645
BssT1I CCWWGG 1 cut(s) 568
Bst4CI ACNGT 4 cut(s) 255, 541, 598, 606
Bst6I CTCTTC 2 cut(s) 77, 663
BstAPI GCANNNNNTGC 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 41, 535
BstDEI CTNAG 3 cut(s) 25, 78, 320
BstDSI CCRYGG 1 cut(s) 568
BstKTI GATC 3 cut(s) 580, 631, 648
BstMAI GTCTC 2 cut(s) 68, 457
BstMBI GATC 3 cut(s) 577, 628, 645
BstMWI GCNNNNNNNGC 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 128
BstV1I GCAGC 4 cut(s) 242, 496, 499, 538
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 645
BstYI RGATCY 1 cut(s) 645
BsuRI GGCC 2 cut(s) 70, 423
BtgI CCRYGG 1 cut(s) 568
BtgZI GCGATG 1 cut(s) 354
BtsIMutI CAGTG 3 cut(s) 321, 478, 537
Cac8I GCNNGC 2 cut(s) 41, 535
CaiI CAGNNNCTG 1 cut(s) 539
Cfr13I GGNCC 2 cut(s) 344, 546
Csp6I GTAC 2 cut(s) 383, 607
CviAII CATG 3 cut(s) 108, 428, 569
CviQI GTAC 2 cut(s) 383, 607
DdeI CTNAG 3 cut(s) 25, 78, 320
DpnI GATC 3 cut(s) 579, 630, 647
DpnII GATC 3 cut(s) 577, 628, 645
DraIII CACNNNGTG 1 cut(s) 637
EaeI YGGCCR 1 cut(s) 68
Eam1104I CTCTTC 2 cut(s) 77, 663
EarI CTCTTC 2 cut(s) 77, 663
Eco130I CCWWGG 1 cut(s) 568
Eco147I AGGCCT 1 cut(s) 423
Eco31I GGTCTC 1 cut(s) 68
Eco32I GATATC 1 cut(s) 195
Eco47I GGWCC 2 cut(s) 344, 546
Eco88I CYCGRG 1 cut(s) 127
EcoRV GATATC 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 568
FaeI CATG 3 cut(s) 111, 431, 572
FatI CATG 3 cut(s) 107, 427, 568
FbaI TGATCA 2 cut(s) 577, 628
Fnu4HI GCNGC 5 cut(s) 6, 231, 510, 513, 552
Fsp4HI GCNGC 5 cut(s) 6, 231, 510, 513, 552
GluI GCNGC 5 cut(s) 6, 231, 510, 513, 552
HaeIII GGCC 2 cut(s) 70, 423
Hin1II CATG 3 cut(s) 111, 431, 572
HinfI GANTC 2 cut(s) 137, 356
HphI GGTGA 2 cut(s) 382, 649
Hpy188I TCNGA 4 cut(s) 54, 81, 435, 487
Hpy188III TCNNGA 3 cut(s) 34, 212, 649
Hpy99I CGWCG 1 cut(s) 174
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 4 cut(s) 255, 541, 598, 606
HpyCH4V TGCA 4 cut(s) 230, 520, 533, 602
HpyF10VI GCNNNNNNNGC 1 cut(s) 123
HpyF3I CTNAG 3 cut(s) 25, 78, 320
Hsp92II CATG 3 cut(s) 111, 431, 572
Ksp22I TGATCA 2 cut(s) 577, 628
Kzo9I GATC 3 cut(s) 577, 628, 645
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 2 cut(s) 208, 572
Lsp1109I GCAGC 4 cut(s) 242, 496, 499, 538
MaeIII GTNAC 2 cut(s) 110, 173
MalI GATC 3 cut(s) 579, 630, 647
MboI GATC 3 cut(s) 577, 628, 645
MboII GAAGA 4 cut(s) 94, 289, 351, 403
MflI RGATCY 1 cut(s) 645
MhlI GDGCHC 1 cut(s) 128
MlsI TGGCCA 1 cut(s) 70
MluCI AATT 6 cut(s) 55, 178, 467, 588, 623, 661
MluNI TGGCCA 1 cut(s) 70
MlyI GAGTC 2 cut(s) 131, 365
MmeI TCCRAC 1 cut(s) 478
MnlI CCTC 3 cut(s) 19, 139, 434
Mox20I TGGCCA 1 cut(s) 70
MscI TGGCCA 1 cut(s) 70
MseI TTAA 2 cut(s) 18, 660
MslI CAYNNNNRTG 1 cut(s) 106
Msp20I TGGCCA 1 cut(s) 70
MspA1I CMGCKG 1 cut(s) 551
MwoI GCNNNNNNNGC 1 cut(s) 123
NcoI CCATGG 1 cut(s) 568
NdeII GATC 3 cut(s) 577, 628, 645
NlaIII CATG 3 cut(s) 111, 431, 572
PceI AGGCCT 1 cut(s) 423
PciSI GCTCTTC 1 cut(s) 77
PflMI CCANNNNNTGG 1 cut(s) 341
PkrI GCNGC 5 cut(s) 7, 232, 511, 514, 553
PleI GAGTC 2 cut(s) 131, 364
PpsI GAGTC 2 cut(s) 131, 364
PspPI GGNCC 2 cut(s) 344, 546
PstNI CAGNNNCTG 1 cut(s) 539
PsuI RGATCY 1 cut(s) 645
RsaI GTAC 2 cut(s) 384, 608
RsaNI GTAC 2 cut(s) 383, 607
RseI CAYNNNNRTG 1 cut(s) 106
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 2 cut(s) 18, 660
SatI GCNGC 5 cut(s) 6, 231, 510, 513, 552
Sau3AI GATC 3 cut(s) 577, 628, 645
Sau96I GGNCC 2 cut(s) 344, 546
SchI GAGTC 2 cut(s) 131, 365
SduI GDGCHC 1 cut(s) 128
SetI ASST 7 cut(s) 24, 79, 134, 150, 377, 511, 577
SinI GGWCC 2 cut(s) 344, 546
SmiMI CAYNNNNRTG 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 6 cut(s) 55, 178, 467, 588, 623, 661
SseBI AGGCCT 1 cut(s) 423
SsiI CCGC 1 cut(s) 549
StuI AGGCCT 1 cut(s) 423
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 4 cut(s) 255, 541, 598, 606
TaqI TCGA 1 cut(s) 182
TasI AATT 6 cut(s) 55, 178, 467, 588, 623, 661
TatI WGTACW 1 cut(s) 606
Tru1I TTAA 2 cut(s) 18, 660
Tru9I TTAA 2 cut(s) 18, 660
TscAI CASTG 3 cut(s) 321, 478, 544
TseI GCWGC 5 cut(s) 5, 230, 509, 512, 551
TspDTI ATGAA 2 cut(s) 177, 351
TspRI CASTG 3 cut(s) 321, 478, 544
Van91I CCANNNNNTGG 1 cut(s) 341
VpaK11BI GGWCC 2 cut(s) 344, 546
XapI RAATTY 2 cut(s) 55, 623
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.