Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008572

Physical Location & Seq

Genomic Coordinates Reverse (-)

31607087 .. 31612505

Gene Structure 5419 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1217900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGGGAACCTCTTGTCACTTTTCAAGAGCAAGCAGAACAGCCCTGCGGTTTCTAGAAGTATCTCCGGTGGCAACGCCGGCAGAAACCACAGTGTCACTGGTAGAACGCCGTCGTATGATCCCGGGATGTTCGGGAAACCGAACATAAATGATACGAGACCTAGCAAAGCACAGAGTGGTACGGAGAAGCAGCTCTCTACCCAGAAGCAGAAGTACGCTTATATCCCTGATAACTTCACATCCCTTCAACAGGTTACAGATGCCTTGAGAAAAGAAGGTTTAGAGTCATCTAATCTCATCGTCGGAATTGACTTCACCAAGAGCAACGAGTGGACAGGCAAAGTTTCATTCAGAAACCGGAGCCTGCACGCCATTGGCGATGAACCTAATCCATACGAGAAAGCTATCTCCATAATTGGAAAAACTTTATCTCCATTTGATGAAGACAACCTCATTCCTTGTTTCGGCTTTGGTGATGCTACCACTCATGATCAAGAGGTGTTTAGCTTTCACAATGATCATTCGCCCTGCCATGGTTTCGAAGAAGTCTTGGCCTGCTACAAAAAAATAGTTCCAAATTTGCATCTTTCGGGGCCAACATCTTATGGACCTGTAGTTGAAGCTGCAATGGATATTGTGGAGAAAAGTGGAGGGCAATTCCATGTTTTAGTTATCATTGCAGATGGCCAGGTCACTAGAAGTATCGATACTAGCGACAATGAATTGAGTCCACAGGAGGAGAAGACGATCAGATCAATTGCAGATGCAAGTTTCTATCCGCTCTCGATTGTTCTTGTTGGAGTCGGGGATGGTCCTTGGGAAGACATGAGGAAGTTCGATGACAAGCTCCCTTCACGCGAATTTGATAATTTTCAGTTCGTTAACTTCACCGAAATTATGTCTAAACACACAACTTCATCCGAGAAAGAAGCTGCATTTGCTCTGGCTGCCCTCATGGAAATCCCAATCCAGTATAAGGCAGCTGTTGAGTTCGGTATACTTGGACGTACGACAGGGAAAAAGAAGAGAATCGTTCCACGCCCTCCACCAGTTCCTTACACCCATCGTGCACCAACTCGCGAACCAAGTGGTCTTTCAGCACCTGCAGGGGATGAACGAAGCGAGATGCTCTGTCCAGTCTGCCTAACTAATGTAAAGGACATTGCCTTTGGTTGCGGACACATGTCGTGCAGAGACTGTGCACCGAGATTGTCCGACTGTCCAATTTGCCGCCAGCCAATTCGCAGCCGTCTCAGGGTTTTCACTGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009308 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009690 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009850 GO:0009867 GO:0009987 GO:0010033 GO:0010817 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023052 GO:0032446 GO:0032870 GO:0034754 GO:0036211 GO:0042221 GO:0042445 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046483 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0065008 GO:0070647 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0080148 GO:0098542 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:2000070

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Copine zf-C3HC4_3

Protein Analysis

424

Amino Acids

46.51

Weight (kDa)

6.61

Isoelectric Point (pI)

45.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Copine	PF07002	121 - 333	3.1e-71	Copine
zf-C3HC4_3	PF13920	375 - 416	8.5e-10	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79380
fragaria_vesca	FvH4_3g13940 FvH4_3g13940 FvH4_3g13940 FvH4_3g13940
malus_domestica	MD05G1239600.v1.1 MD10G1217900.v1.1
prunus_persica	Prupe.4G126200_v2.0.a1 Prupe.4G126200_v2.0.a1
pyrus_communis	pycom05g21590 pycom10g18370
rosa_chinensis	RchiOBHm_Chr5g0023161
rosa_laevigata	RLG00000032704
rosa_multiflora	Rmu_sc0007300.1_g000015
rosa_roxburghii	Rroxscaffold_1G00056180
rosa_rugosa	Rorug05G0077700 Rorug05G0077700 Rorug05G0077700
rosa_samantha	Rh5AG163800 Rh5BG163100 Rh5CG178100 Rh5DG164500
rosa_wichuraiana	Rw5G014780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1111
Acc36I	ACCTGC	1 cut(s)	1111
AccBSI	CCGCTC	1 cut(s)	781
AccI	GTMKAC	1 cut(s)	997
AccII	CGCG	2 cut(s)	858, 1080
AciI	CCGC	4 cut(s)	47, 779, 1176, 1231
AclWI	GGATC	1 cut(s)	113
AcoI	YGGCCR	1 cut(s)	685
AcsI	RAATTY	2 cut(s)	577, 860
AdeI	CACNNNGTG	1 cut(s)	176
AfaI	GTAC	3 cut(s)	181, 215, 1009
AfiI	CCNNNNNNNGG	5 cut(s)	250, 464, 533, 976, 1255
AflIII	ACRYGT	1 cut(s)	1182
AgsI	TTSAA	3 cut(s)	25, 248, 620
AjnI	CCWGG	1 cut(s)	687
AjuI	GAANNNNNNNTTGG	2 cut(s)	1078, 1110
AluBI	AGCT	7 cut(s)	193, 404, 507, 623, 847, 932, 983
AluI	AGCT	7 cut(s)	193, 404, 507, 623, 847, 932, 983
Alw21I	GWGCWC	2 cut(s)	1072, 1204
Alw26I	GTCTC	3 cut(s)	151, 1188, 1256
Alw44I	GTGCAC	2 cut(s)	1068, 1200
AlwI	GGATC	1 cut(s)	113
AlwNI	CAGNNNCTG	2 cut(s)	1103, 1197
Ama87I	CYCGRG	1 cut(s)	122
AoxI	GGCC	3 cut(s)	552, 593, 685
ApaLI	GTGCAC	2 cut(s)	1068, 1200
ApeKI	GCWGC	6 cut(s)	190, 623, 932, 947, 980, 1245
ApoI	RAATTY	2 cut(s)	577, 860
ArsI	GACNNNNNNTTYG	2 cut(s)	1151, 1183
AspS9I	GGNCC	3 cut(s)	593, 608, 812
AsuC2I	CCSGG	2 cut(s)	123, 124
AsuHPI	GGTGA	3 cut(s)	307, 485, 880
AsuII	TTCGAA	1 cut(s)	540
AvaI	CYCGRG	1 cut(s)	122
AvaII	GGWCC	2 cut(s)	608, 812
BaeGI	GKGCMC	2 cut(s)	1072, 1204
BalI	TGGCCA	1 cut(s)	687
BarI	GAAGNNNNNNTAC	4 cut(s)	197, 229, 691, 723
BbsI	GAAGAC	3 cut(s)	450, 749, 828
Bbv12I	GWGCWC	2 cut(s)	1072, 1204
BbvI	GCAGC	6 cut(s)	202, 610, 919, 934, 992, 1257
BccI	CCATC	3 cut(s)	677, 803, 1071
BceAI	ACGGC	2 cut(s)	94, 1233
BciT130I	CCWGG	1 cut(s)	689
BclI	TGATCA	2 cut(s)	490, 517
BcnI	CCSGG	2 cut(s)	123, 124
BcoDI	GTCTC	3 cut(s)	151, 1188, 1256
BfaI	CTAG	4 cut(s)	54, 162, 696, 711
BfmI	CTRYAG	2 cut(s)	612, 1104
BfuAI	ACCTGC	1 cut(s)	1111
BisI	GCNGC	7 cut(s)	191, 624, 933, 948, 981, 1231, 1246
BlsI	GCNGC	7 cut(s)	192, 625, 934, 949, 982, 1232, 1247
Bme1390I	CCNGG	3 cut(s)	123, 124, 689
Bme18I	GGWCC	2 cut(s)	608, 812
BmeT110I	CYCGRG	1 cut(s)	122
BmgT120I	GGNCC	3 cut(s)	593, 608, 812
BmiI	GGNNCC	3 cut(s)	8, 362, 594
BmrFI	CCNGG	3 cut(s)	123, 124, 689
BmsI	GCATC	5 cut(s)	250, 466, 592, 754, 1116
BoxI	GACNNNNGTC	1 cut(s)	1183
BpiI	GAAGAC	3 cut(s)	450, 749, 828
Bpu14I	TTCGAA	1 cut(s)	540
BpuEI	CTTGAG	1 cut(s)	286
BpuMI	CCSGG	2 cut(s)	123, 124
Bsa29I	ATCGAT	1 cut(s)	705
BsaI	GGTCTC	1 cut(s)	151
BsaJI	CCNNGG	3 cut(s)	122, 532, 815
BsaWI	WCCGGW	2 cut(s)	65, 357
BsaXI	ACNNNNNCTCC	2 cut(s)	415, 445
Bsc4I	CCNNNNNNNGG	5 cut(s)	250, 464, 533, 976, 1255
Bse118I	RCCGGY	1 cut(s)	77
Bse1I	ACTGG	5 cut(s)	103, 970, 1049, 1136, 1270
Bse3DI	GCAATG	3 cut(s)	633, 675, 1161
BseBI	CCWGG	1 cut(s)	689
BseCI	ATCGAT	1 cut(s)	705
BseDI	CCNNGG	3 cut(s)	122, 532, 815
BseGI	GGATG	5 cut(s)	132, 239, 814, 917, 1117
BseLI	CCNNNNNNNGG	5 cut(s)	250, 464, 533, 976, 1255
BseMI	GCAATG	3 cut(s)	633, 675, 1161
BseMII	CTCAG	1 cut(s)	1267
BseNI	ACTGG	5 cut(s)	103, 970, 1049, 1136, 1270
BseRI	GAGGAG	1 cut(s)	752
BseSI	GKGCMC	2 cut(s)	1072, 1204
BseXI	GCAGC	6 cut(s)	202, 610, 919, 934, 992, 1257
BsgI	GTGCAG	2 cut(s)	350, 1210
Bsh1236I	CGCG	2 cut(s)	858, 1080
BshFI	GGCC	3 cut(s)	554, 595, 687
BshVI	ATCGAT	1 cut(s)	705
BsiHKAI	GWGCWC	2 cut(s)	1072, 1204
BsiHKCI	CYCGRG	1 cut(s)	122
BsiSI	CCGG	4 cut(s)	66, 78, 123, 358
BsiWI	CGTACG	1 cut(s)	1007
BslI	CCNNNNNNNGG	5 cut(s)	250, 464, 533, 976, 1255
BsmAI	GTCTC	3 cut(s)	151, 1188, 1256
BsmBI	CGTCTC	1 cut(s)	1256
BsnI	GGCC	3 cut(s)	554, 595, 687
Bso31I	GGTCTC	1 cut(s)	151
BsoBI	CYCGRG	1 cut(s)	122
Bsp119I	TTCGAA	1 cut(s)	540
Bsp1286I	GDGCHC	2 cut(s)	1072, 1204
Bsp143I	GATC	5 cut(s)	118, 490, 517, 747, 752
Bsp19I	CCATGG	1 cut(s)	532
Bsp68I	TCGCGA	1 cut(s)	1080
BspACI	CCGC	4 cut(s)	47, 779, 1176, 1231
BspANI	GGCC	3 cut(s)	554, 595, 687
BspCNI	CTCAG	1 cut(s)	1266
BspDI	ATCGAT	1 cut(s)	705
BspFNI	CGCG	2 cut(s)	858, 1080
BspHI	TCATGA	1 cut(s)	487
BspLI	GGNNCC	3 cut(s)	8, 362, 594
BspMAI	CTGCAG	1 cut(s)	1108
BspMI	ACCTGC	1 cut(s)	1111
BspPI	GGATC	1 cut(s)	113
BspT104I	TTCGAA	1 cut(s)	540
BspTNI	GGTCTC	1 cut(s)	151
BsrBI	CCGCTC	1 cut(s)	781
BsrDI	GCAATG	3 cut(s)	633, 675, 1161
BsrFI	RCCGGY	1 cut(s)	77
BsrI	ACTGG	5 cut(s)	103, 970, 1049, 1136, 1270
BssAI	RCCGGY	1 cut(s)	77
BssECI	CCNNGG	3 cut(s)	122, 532, 815
BssMI	GATC	5 cut(s)	118, 490, 517, 747, 752
BssNAI	GTATAC	1 cut(s)	998
BssT1I	CCWWGG	2 cut(s)	532, 815
Bst1107I	GTATAC	1 cut(s)	998
Bst2UI	CCWGG	1 cut(s)	689
Bst4CI	ACNGT	3 cut(s)	92, 1199, 1220
Bst6I	CTCTTC	1 cut(s)	1019
BstBI	TTCGAA	1 cut(s)	540
BstC8I	GCNNGC	6 cut(s)	32, 79, 365, 369, 556, 1235
BstDEI	CTNAG	1 cut(s)	1253
BstDSI	CCRYGG	1 cut(s)	532
BstENI	CCTNNNNNAGG	1 cut(s)	248
BstF5I	GGATG	5 cut(s)	132, 239, 814, 917, 1117
BstFNI	CGCG	2 cut(s)	858, 1080
BstKTI	GATC	5 cut(s)	121, 493, 520, 750, 755
BstMAI	GTCTC	3 cut(s)	151, 1188, 1256
BstMBI	GATC	5 cut(s)	118, 490, 517, 747, 752
BstMWI	GCNNNNNNNGC	3 cut(s)	78, 938, 947
BstNI	CCWGG	1 cut(s)	689
BstNSI	RCATGY	1 cut(s)	1186
BstPAI	GACNNNNGTC	1 cut(s)	1183
BstSCI	CCNGG	3 cut(s)	121, 122, 687
BstSFI	CTRYAG	2 cut(s)	612, 1104
BstSLI	GKGCMC	2 cut(s)	1072, 1204
BstUI	CGCG	2 cut(s)	858, 1080
BstV1I	GCAGC	6 cut(s)	202, 610, 919, 934, 992, 1257
BstV2I	GAAGAC	3 cut(s)	450, 749, 828
BstZ17I	GTATAC	1 cut(s)	998
Bsu15I	ATCGAT	1 cut(s)	705
BsuRI	GGCC	3 cut(s)	554, 595, 687
BsuTUI	ATCGAT	1 cut(s)	705
BtgI	CCRYGG	1 cut(s)	532
BtgZI	GCGATG	1 cut(s)	393
BtsCI	GGATG	5 cut(s)	132, 239, 814, 917, 1117
BtsIMutI	CAGTG	3 cut(s)	96, 97, 1263
BtuMI	TCGCGA	1 cut(s)	1080
BveI	ACCTGC	1 cut(s)	1111
Cac8I	GCNNGC	6 cut(s)	32, 79, 365, 369, 556, 1235
CaiI	CAGNNNCTG	2 cut(s)	1103, 1197
CciI	TCATGA	1 cut(s)	487
Cfr10I	RCCGGY	1 cut(s)	77
Cfr13I	GGNCC	3 cut(s)	593, 608, 812
Cfr9I	CCCGGG	1 cut(s)	122
ClaI	ATCGAT	1 cut(s)	705
Csp6I	GTAC	3 cut(s)	180, 214, 1008
CviAII	CATG	6 cut(s)	488, 533, 662, 826, 955, 1183
CviQI	GTAC	3 cut(s)	180, 214, 1008
DdeI	CTNAG	1 cut(s)	1253
DpnI	GATC	5 cut(s)	120, 492, 519, 749, 754
DpnII	GATC	5 cut(s)	118, 490, 517, 747, 752
DraIII	CACNNNGTG	1 cut(s)	176
EaeI	YGGCCR	1 cut(s)	685
Eam1104I	CTCTTC	1 cut(s)	1019
EarI	CTCTTC	1 cut(s)	1019
Eco130I	CCWWGG	2 cut(s)	532, 815
Eco31I	GGTCTC	1 cut(s)	151
Eco47I	GGWCC	2 cut(s)	608, 812
Eco88I	CYCGRG	1 cut(s)	122
EcoNI	CCTNNNNNAGG	1 cut(s)	248
EcoRII	CCWGG	1 cut(s)	687
EcoT14I	CCWWGG	2 cut(s)	532, 815
ErhI	CCWWGG	2 cut(s)	532, 815
Esp3I	CGTCTC	1 cut(s)	1256
FaeI	CATG	6 cut(s)	491, 536, 665, 829, 958, 1186
FatI	CATG	6 cut(s)	487, 532, 661, 825, 954, 1182
FbaI	TGATCA	2 cut(s)	490, 517
FblI	GTMKAC	1 cut(s)	997
Fnu4HI	GCNGC	7 cut(s)	191, 624, 933, 948, 981, 1231, 1246
FokI	GGATG	5 cut(s)	139, 226, 821, 904, 1124
Fsp4HI	GCNGC	7 cut(s)	191, 624, 933, 948, 981, 1231, 1246
FspBI	CTAG	4 cut(s)	54, 162, 696, 711
GluI	GCNGC	7 cut(s)	191, 624, 933, 948, 981, 1231, 1246
HaeIII	GGCC	3 cut(s)	554, 595, 687
HapII	CCGG	4 cut(s)	66, 78, 123, 358
Hin1II	CATG	6 cut(s)	491, 536, 665, 829, 958, 1186
HincII	GTYRAC	1 cut(s)	883
HindII	GTYRAC	1 cut(s)	883
HinfI	GANTC	4 cut(s)	284, 727, 801, 1029
HpaI	GTTAAC	1 cut(s)	883
HpaII	CCGG	4 cut(s)	66, 78, 123, 358
HphI	GGTGA	3 cut(s)	307, 485, 880
Hpy166II	GTNNAC	6 cut(s)	333, 731, 883, 998, 1070, 1202
Hpy188I	TCNGA	5 cut(s)	305, 353, 752, 922, 1216
Hpy188III	TCNNGA	7 cut(s)	25, 54, 133, 488, 494, 784, 1079
Hpy8I	GTNNAC	6 cut(s)	333, 731, 883, 998, 1070, 1202
Hpy99I	CGWCG	2 cut(s)	115, 305
HpyAV	CCTTC	3 cut(s)	254, 269, 861
HpyCH4III	ACNGT	3 cut(s)	92, 1199, 1220
HpyCH4IV	ACGT	1 cut(s)	1006
HpyF10VI	GCNNNNNNNGC	3 cut(s)	78, 938, 947
HpyF3I	CTNAG	1 cut(s)	1253
HpySE526I	ACGT	1 cut(s)	1006
Hsp92II	CATG	6 cut(s)	491, 536, 665, 829, 958, 1186
KroI	GCCGGC	1 cut(s)	77
KroNI	GCCGGC	1 cut(s)	79
Ksp22I	TGATCA	2 cut(s)	490, 517
KspAI	GTTAAC	1 cut(s)	883
Kzo9I	GATC	5 cut(s)	118, 490, 517, 747, 752
LmnI	GCTCC	2 cut(s)	360, 852
Lsp1109I	GCAGC	6 cut(s)	202, 610, 919, 934, 992, 1257
LweI	GCATC	5 cut(s)	250, 466, 592, 754, 1116
MaeI	CTAG	4 cut(s)	54, 162, 696, 711
MaeII	ACGT	1 cut(s)	1006
MaeIII	GTNAC	4 cut(s)	15, 94, 253, 691
MalI	GATC	5 cut(s)	120, 492, 519, 749, 754
MbiI	CCGCTC	1 cut(s)	781
MboI	GATC	5 cut(s)	118, 490, 517, 747, 752
MboII	GAAGA	5 cut(s)	455, 554, 754, 833, 1036
MfeI	CAATTG	1 cut(s)	756
MhlI	GDGCHC	2 cut(s)	1072, 1204
MlsI	TGGCCA	1 cut(s)	687
MluNI	TGGCCA	1 cut(s)	687
MlyI	GAGTC	3 cut(s)	293, 736, 810
MmeI	TCCRAC	3 cut(s)	283, 778, 1239
MnlI	CCTC	8 cut(s)	20, 461, 490, 644, 730, 822, 962, 1053
Mox20I	TGGCCA	1 cut(s)	687
MroNI	GCCGGC	1 cut(s)	77
MscI	TGGCCA	1 cut(s)	687
MseI	TTAA	1 cut(s)	882
Msp20I	TGGCCA	1 cut(s)	687
MspA1I	CMGCKG	1 cut(s)	983
MspI	CCGG	4 cut(s)	66, 78, 123, 358
MspR9I	CCNGG	3 cut(s)	123, 124, 689
MunI	CAATTG	1 cut(s)	756
MvaI	CCWGG	1 cut(s)	689
MvnI	CGCG	2 cut(s)	858, 1080
MwoI	GCNNNNNNNGC	3 cut(s)	78, 938, 947
NaeI	GCCGGC	1 cut(s)	79
NciI	CCSGG	2 cut(s)	123, 124
NcoI	CCATGG	1 cut(s)	532
NdeII	GATC	5 cut(s)	118, 490, 517, 747, 752
NgoMIV	GCCGGC	1 cut(s)	77
NlaIII	CATG	6 cut(s)	491, 536, 665, 829, 958, 1186
NlaIV	GGNNCC	3 cut(s)	8, 362, 594
NmuCI	GTSAC	3 cut(s)	15, 94, 691
NruI	TCGCGA	1 cut(s)	1080
NspI	RCATGY	1 cut(s)	1186
NspV	TTCGAA	1 cut(s)	540
PagI	TCATGA	1 cut(s)	487
PaqCI	CACCTGC	1 cut(s)	1111
PciI	ACATGT	1 cut(s)	1182
PcsI	WCGNNNNNNNCGW	2 cut(s)	375, 711
PdiI	GCCGGC	1 cut(s)	79
PfeI	GAWTC	1 cut(s)	1029
Pfl23II	CGTACG	1 cut(s)	1007
PkrI	GCNGC	7 cut(s)	192, 625, 934, 949, 982, 1232, 1247
PleI	GAGTC	3 cut(s)	292, 735, 809
PpsI	GAGTC	3 cut(s)	292, 735, 809
PscI	ACATGT	1 cut(s)	1182
PshAI	GACNNNNGTC	1 cut(s)	1183
Psp6I	CCWGG	1 cut(s)	687
PspGI	CCWGG	1 cut(s)	687
PspLI	CGTACG	1 cut(s)	1007
PspN4I	GGNNCC	3 cut(s)	8, 362, 594
PspPI	GGNCC	3 cut(s)	593, 608, 812
PstI	CTGCAG	1 cut(s)	1108
PstNI	CAGNNNCTG	2 cut(s)	1103, 1197
PvuII	CAGCTG	1 cut(s)	983
RruI	TCGCGA	1 cut(s)	1080
RsaI	GTAC	3 cut(s)	181, 215, 1009
RsaNI	GTAC	3 cut(s)	180, 214, 1008
SaqAI	TTAA	1 cut(s)	882
SatI	GCNGC	7 cut(s)	191, 624, 933, 948, 981, 1231, 1246
Sau3AI	GATC	5 cut(s)	118, 490, 517, 747, 752
Sau96I	GGNCC	3 cut(s)	593, 608, 812
SbfI	CCTGCAGG	1 cut(s)	1108
SchI	GAGTC	3 cut(s)	293, 736, 810
ScrFI	CCNGG	3 cut(s)	123, 124, 689
SdaI	CCTGCAGG	1 cut(s)	1108
SduI	GDGCHC	2 cut(s)	1072, 1204
SfaNI	GCATC	5 cut(s)	250, 466, 592, 754, 1116
SfcI	CTRYAG	2 cut(s)	612, 1104
SfuI	TTCGAA	1 cut(s)	540
SinI	GGWCC	2 cut(s)	608, 812
SmaI	CCCGGG	1 cut(s)	124
SmlI	CTYRAG	1 cut(s)	265
SmoI	CTYRAG	1 cut(s)	265
Sse8387I	CCTGCAGG	1 cut(s)	1108
SsiI	CCGC	4 cut(s)	47, 779, 1176, 1231
SspMI	CTAG	4 cut(s)	54, 162, 696, 711
StyD4I	CCNGG	3 cut(s)	121, 122, 687
StyI	CCWWGG	2 cut(s)	532, 815
TaaI	ACNGT	3 cut(s)	92, 1199, 1220
TaiI	ACGT	1 cut(s)	1009
TaqI	TCGA	4 cut(s)	540, 705, 785, 837
TauI	GCSGC	1 cut(s)	1233
TfiI	GAWTC	1 cut(s)	1029
Tru1I	TTAA	1 cut(s)	882
Tru9I	TTAA	1 cut(s)	882
TscAI	CASTG	3 cut(s)	97, 103, 1270
TseFI	GTSAC	3 cut(s)	15, 94, 691
TseI	GCWGC	6 cut(s)	190, 623, 932, 947, 980, 1245
Tsp45I	GTSAC	3 cut(s)	15, 94, 691
TspDTI	ATGAA	6 cut(s)	336, 396, 456, 735, 906, 1128
TspGWI	ACGGA	1 cut(s)	197
TspMI	CCCGGG	1 cut(s)	122
TspRI	CASTG	3 cut(s)	97, 103, 1270
VneI	GTGCAC	2 cut(s)	1068, 1200
VpaK11BI	GGWCC	2 cut(s)	608, 812
XagI	CCTNNNNNAGG	1 cut(s)	248
XapI	RAATTY	2 cut(s)	577, 860
XbaI	TCTAGA	1 cut(s)	53
XceI	RCATGY	1 cut(s)	1186
XcmI	CCANNNNNNNNNTGG	1 cut(s)	95
XmaI	CCCGGG	1 cut(s)	122
XmiI	GTMKAC	1 cut(s)	997
XspI	CTAG	4 cut(s)	54, 162, 696, 711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.