Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006891

Physical Location & Seq

Genomic Coordinates Forward (+)

31697785 .. 31701140

Gene Structure 3356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1219300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGTCTTCAAATCCATCCAATTTTTCTGACTCTTCCGAGATGAGCTATAGCTCATCAGAAGAAGCACAGTGTGGAACCAAGATGGAATTTCAGGTCAAGAGTGGTGCCAATTCATCGAATGATAAGGTTAACTGCCCCAAAAATGTGGATAGAATAGGAAAGTTTAATGCCAGTTCAACTAATAATCCATCAGGTGGACCTCAGAAAGTGGATCGAGTTTCCAAAGGAACAGAACTCAGTACTAAGACTACGGCTGAGACAACCACCAACAATCGTCCATTTCCAAGCCCAATGGCACCTGCAAATTCTGAACTTCTAACCACTTCAACAGGAAGGGCTACAGGAGGTATGAAAAGTAGCAATTCTGTCACCAACCTTACCCAAATGAGCGGCAGTAGCTCCCGCTCTGACAGCTTAGAGAGCTCCTCCAGTACACCCCTCAGGCCTCATACTGGTGGTGATGTGCGGTGGGATGCCATTAACATGGTCTCTAAAGGTTCCCCACTTAATCTTAGCCATTTTCGGCTTCTCAAGCGCCTAGGATATGGAGATATTGGCAGTGTCTATCTTGTTGAACTCAGAGGAACAAACACGTTTTTTGCCATGAAGGTCATGGACAAGGCATCTCTTGCTAGTAGAAACAAGCTGTTGCGAGCACAGACAGAAAGAGAGATACTTGGACTTCTTGACCACCCATTCTTGCCCACTCTCTATTCTTACTTTGAAACTGATAAGTTCTATTGCTTGGTCATGGAGTACTGTAGTGGAGGTAATCTACACTCTCTTCGGCAGAAGCAACCAAACAAGCATTTTACAGAGGAAGCTTCACGGTTTTATGCATCACAGGTTTTGTTAGCACTTGAGTATCTGCACATGCTAGGAATTGTGTACAGGGATTTAAAACCAGAAAATGTTCTAGTAAGAGACGAGGGTCATATCATGCTCTCAGACTTCGATCTCTCACTCCGTTGTTCTGTCAATCCTACACTAGTCAAGTCTTCATCTGCCCATGTAAGCAATGGTGGTGCTACCGGTGGTGGTGGTGGTGGCGGGGGCATTTTAGACGATGACCTTGCTGTGCATGGTTGTATGCAGCCTTCCAATTTCTTTCCACGCATTTTACCTACCAAGAAAAACCGGAAATCCAAATCAGATTTTGGCCTTTCAGCGAATGGATCCCTCCCTGAACTGATGGCAGAGCCTACAAATGTGCGTTCCATGTCATTTGTTGGAACACACGAATATCTAGCCCCAGAGATTATTCGCGGAGAGGGCCATGGTAGTGCAGTGGACTGGTGGACATTTGGTATCTTCATATACGAGCTCTTACATGGGACAACCCCCTTCAAAGGTCAAGGAAATCGTGCCACACTCTTCAACGTTGTAGGCCAGCCTCTGAAATTCCCAGAAACACCACCAGTAAGTGTTGTGGCACGTAATCTCATACGAGGACTCTTGGTGAAAGAACCACATAAACGAATTGCGTACAAAAGGGGTGCCACAGAAATAAAGCAACACCCATTTTTCGAGGGAGTGAACTGGGCTCTGGTGAGATGTGCCACGCCTCCCCATGTTCCTGAACCGGTAGACTTTTCACAATATGCCAGCAAGGAGGCGAACTCTGCAGACAAAAAGATGACAGACGTGGGAAGTGATAAGAAGAACGGTAGTAATGATTCATCTTATGTAAATTTTGAGTACTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044706 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051716 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:0140096 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

569

Amino Acids

62.09

Weight (kDa)

8.73

Isoelectric Point (pI)

41.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	175 - 325	1.8e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	175 - 327	1.4e-19	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	384 - 509	1.8e-21	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	384 - 494	3.3e-06	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006891)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79250 AT1G79250 AT1G79250 AT1G79250
fragaria_vesca	FvH4_3g13780 FvH4_3g13780 FvH4_3g13780
malus_domestica	MD05G1241000.v1.1 MD10G1219300.v1.1
prunus_persica	Prupe.4G124600_v2.0.a1 Prupe.4G124600_v2.0.a1 Prupe.4G124600_v2.0.a1
pyrus_communis	pycom05g21720 pycom10g18490
rosa_chinensis	RchiOBHm_Chr5g0022971
rosa_laevigata	RLG00000032685
rosa_multiflora	Rmu_sc0002675.1_g000004
rosa_roxburghii	Rroxscaffold_1G00056370
rosa_rugosa	Rorug05G0076000 Rorug05G0076100.1 Rorug05G0076200
rosa_samantha	Rh5AG162300 Rh5BG161500 Rh5CG176200 Rh5DG162900
rosa_wichuraiana	Rw5G014620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	307
Acc36I	ACCTGC	1 cut(s)	307
AccB1I	GGYRCC	3 cut(s)	104, 295, 1496
AccB7I	CCANNNNNTGG	1 cut(s)	194
AccBSI	CCGCTC	2 cut(s)	390, 405
AccI	GTMKAC	1 cut(s)	1587
AccII	CGCG	1 cut(s)	1266
AciI	CCGC	5 cut(s)	390, 403, 466, 1050, 1266
AclI	AACGTT	1 cut(s)	1380
AclWI	GGATC	3 cut(s)	219, 1170, 1183
AcsI	RAATTY	4 cut(s)	86, 304, 1400, 1690
AdeI	CACNNNGTG	1 cut(s)	71
AfaI	GTAC	6 cut(s)	241, 433, 758, 890, 1487, 1700
AfiI	CCNNNNNNNGG	3 cut(s)	194, 452, 1349
AflIII	ACRYGT	1 cut(s)	591
AgeI	ACCGGT	2 cut(s)	1031, 1582
AgsI	TTSAA	7 cut(s)	9, 177, 327, 575, 725, 1348, 1378
AhlI	ACTAGT	1 cut(s)	988
AjiI	CACGTC	1 cut(s)	1645
AluBI	AGCT	8 cut(s)	45, 51, 399, 414, 423, 646, 824, 1324
AluI	AGCT	8 cut(s)	45, 51, 399, 414, 423, 646, 824, 1324
Alw21I	GWGCWC	3 cut(s)	425, 658, 1326
Alw26I	GTCTC	3 cut(s)	251, 493, 918
AlwI	GGATC	3 cut(s)	219, 1170, 1183
AlwNI	CAGNNNCTG	1 cut(s)	1396
AoxI	GGCC	4 cut(s)	443, 1159, 1273, 1387
ApeKI	GCWGC	1 cut(s)	1093
ApoI	RAATTY	4 cut(s)	86, 304, 1400, 1690
AsiGI	ACCGGT	2 cut(s)	1031, 1582
Asp700I	GAANNNNTTC	1 cut(s)	912
AspA2I	CCTAGG	1 cut(s)	538
AspLEI	GCGC	1 cut(s)	537
AspS9I	GGNCC	2 cut(s)	197, 1273
AsuHPI	GGTGA	4 cut(s)	361, 470, 1471, 1561
AvaII	GGWCC	1 cut(s)	197
AvrII	CCTAGG	1 cut(s)	538
AxyI	CCTNAGG	1 cut(s)	440
BaeI	ACNNNNGTAYC	2 cut(s)	848, 881
BamHI	GGATCC	1 cut(s)	1175
BanI	GGYRCC	3 cut(s)	104, 295, 1496
BanII	GRGCYC	3 cut(s)	425, 1326, 1546
BarI	GAAGNNNNNNTAC	2 cut(s)	1081, 1113
BbsI	GAAGAC	1 cut(s)	990
Bbv12I	GWGCWC	3 cut(s)	425, 658, 1326
BbvI	GCAGC	1 cut(s)	1105
BccI	CCATC	4 cut(s)	22, 76, 196, 1186
BceAI	ACGGC	1 cut(s)	267
BcgI	CGANNNNNNTGC	2 cut(s)	1055, 1089
BcoDI	GTCTC	3 cut(s)	251, 493, 918
BcuI	ACTAGT	1 cut(s)	988
BfaI	CTAG	7 cut(s)	539, 633, 878, 917, 989, 1247, 1705
BfmI	CTRYAG	4 cut(s)	46, 339, 760, 1623
BfoI	RGCGCY	1 cut(s)	538
BfuAI	ACCTGC	1 cut(s)	307
BisI	GCNGC	2 cut(s)	391, 1094
BlnI	CCTAGG	1 cut(s)	538
BlsI	GCNGC	2 cut(s)	392, 1095
BmcAI	AGTACT	3 cut(s)	241, 758, 1700
Bme18I	GGWCC	1 cut(s)	197
BmgBI	CACGTC	1 cut(s)	1645
BmgT120I	GGNCC	2 cut(s)	197, 1273
BmiI	GGNNCC	6 cut(s)	76, 106, 297, 499, 1177, 1498
BmrI	ACTGGG	1 cut(s)	1549
BmsI	GCATC	3 cut(s)	463, 632, 848
BmuI	ACTGGG	1 cut(s)	1549
BoxI	GACNNNNGTC	1 cut(s)	930
BpiI	GAAGAC	1 cut(s)	990
BplI	GAGNNNNNCTC	4 cut(s)	410, 442, 1604, 1636
BpmI	CTGGAG	1 cut(s)	412
BpuEI	CTTGAG	2 cut(s)	515, 881
BsaAI	YACGTR	1 cut(s)	1436
BsaI	GGTCTC	1 cut(s)	493
BsaJI	CCNNGG	2 cut(s)	538, 1276
BsaWI	WCCGGW	3 cut(s)	1031, 1137, 1582
Bsc4I	CCNNNNNNNGG	3 cut(s)	194, 452, 1349
Bse118I	RCCGGY	2 cut(s)	1031, 1582
Bse1I	ACTGG	6 cut(s)	171, 429, 457, 1298, 1418, 1544
Bse21I	CCTNAGG	1 cut(s)	440
Bse3DI	GCAATG	1 cut(s)	1024
BseDI	CCNNGG	2 cut(s)	538, 1276
BseGI	GGATG	2 cut(s)	14, 478
BseLI	CCNNNNNNNGG	3 cut(s)	194, 452, 1349
BseMI	GCAATG	1 cut(s)	1024
BseMII	CTCAG	6 cut(s)	215, 246, 250, 454, 592, 960
BseNI	ACTGG	6 cut(s)	171, 429, 457, 1298, 1418, 1544
BseRI	GAGGAG	1 cut(s)	415
BseXI	GCAGC	1 cut(s)	1105
BsgI	GTGCAG	2 cut(s)	854, 1305
Bsh1236I	CGCG	1 cut(s)	1266
BshFI	GGCC	4 cut(s)	445, 1161, 1275, 1389
BshNI	GGYRCC	3 cut(s)	104, 295, 1496
BshTI	ACCGGT	2 cut(s)	1031, 1582
BsiHKAI	GWGCWC	3 cut(s)	425, 658, 1326
BsiSI	CCGG	3 cut(s)	1032, 1138, 1583
BslFI	GGGAC	1 cut(s)	1348
BslI	CCNNNNNNNGG	3 cut(s)	194, 452, 1349
BsmAI	GTCTC	3 cut(s)	251, 493, 918
BsmBI	CGTCTC	1 cut(s)	918
BsmFI	GGGAC	1 cut(s)	1348
BsnI	GGCC	4 cut(s)	445, 1161, 1275, 1389
Bso31I	GGTCTC	1 cut(s)	493
Bsp1286I	GDGCHC	4 cut(s)	425, 658, 1326, 1546
Bsp1407I	TGTACA	1 cut(s)	888
Bsp143I	GATC	3 cut(s)	211, 955, 1175
Bsp19I	CCATGG	1 cut(s)	1276
BspACI	CCGC	5 cut(s)	390, 403, 466, 1050, 1266
BspANI	GGCC	4 cut(s)	445, 1161, 1275, 1389
BspCNI	CTCAG	6 cut(s)	214, 247, 249, 453, 591, 959
BspFNI	CGCG	1 cut(s)	1266
BspLI	GGNNCC	6 cut(s)	76, 106, 297, 499, 1177, 1498
BspMAI	CTGCAG	1 cut(s)	1627
BspMI	ACCTGC	1 cut(s)	307
BspPI	GGATC	3 cut(s)	219, 1170, 1183
BspT107I	GGYRCC	3 cut(s)	104, 295, 1496
BspTNI	GGTCTC	1 cut(s)	493
BsrBI	CCGCTC	2 cut(s)	390, 405
BsrDI	GCAATG	1 cut(s)	1024
BsrFI	RCCGGY	2 cut(s)	1031, 1582
BsrGI	TGTACA	1 cut(s)	888
BsrI	ACTGG	6 cut(s)	171, 429, 457, 1298, 1418, 1544
BssAI	RCCGGY	2 cut(s)	1031, 1582
BssECI	CCNNGG	2 cut(s)	538, 1276
BssMI	GATC	3 cut(s)	211, 955, 1175
BssT1I	CCWWGG	2 cut(s)	538, 1276
Bst4CI	ACNGT	4 cut(s)	69, 761, 831, 1667
Bst6I	CTCTTC	3 cut(s)	37, 789, 1379
BstAPI	GCANNNNNTGC	1 cut(s)	629
BstAUI	TGTACA	1 cut(s)	888
BstBAI	YACGTR	1 cut(s)	1436
BstC8I	GCNNGC	3 cut(s)	654, 1391, 1606
BstDEI	CTNAG	9 cut(s)	201, 236, 243, 255, 415, 440, 512, 578, 946
BstDSI	CCRYGG	1 cut(s)	1276
BstF5I	GGATG	2 cut(s)	14, 478
BstFNI	CGCG	1 cut(s)	1266
BstH2I	RGCGCY	1 cut(s)	538
BstHHI	GCGC	1 cut(s)	537
BstKTI	GATC	3 cut(s)	214, 958, 1178
BstMAI	GTCTC	3 cut(s)	251, 493, 918
BstMBI	GATC	3 cut(s)	211, 955, 1175
BstMWI	GCNNNNNNNGC	7 cut(s)	396, 411, 420, 532, 629, 1272, 1622
BstNSI	RCATGY	1 cut(s)	877
BstPAI	GACNNNNGTC	1 cut(s)	930
BstSFI	CTRYAG	4 cut(s)	46, 339, 760, 1623
BstUI	CGCG	1 cut(s)	1266
BstV1I	GCAGC	1 cut(s)	1105
BstV2I	GAAGAC	1 cut(s)	990
BstX2I	RGATCY	1 cut(s)	1175
BstXI	CCANNNNNNTGG	2 cut(s)	145, 484
BstYI	RGATCY	1 cut(s)	1175
Bsu36I	CCTNAGG	1 cut(s)	440
BsuRI	GGCC	4 cut(s)	445, 1161, 1275, 1389
BtgI	CCRYGG	1 cut(s)	1276
BtrI	CACGTC	1 cut(s)	1645
BtsCI	GGATG	2 cut(s)	14, 478
BtsI	GCAGTG	2 cut(s)	565, 1293
BtsIMutI	CAGTG	3 cut(s)	74, 565, 1293
BveI	ACCTGC	1 cut(s)	307
Cac8I	GCNNGC	3 cut(s)	654, 1391, 1606
CaiI	CAGNNNCTG	1 cut(s)	1396
CfoI	GCGC	1 cut(s)	537
Cfr10I	RCCGGY	2 cut(s)	1031, 1582
Cfr13I	GGNCC	2 cut(s)	197, 1273
Csp6I	GTAC	6 cut(s)	240, 432, 757, 889, 1486, 1699
CspAI	ACCGGT	2 cut(s)	1031, 1582
CviQI	GTAC	6 cut(s)	240, 432, 757, 889, 1486, 1699
DdeI	CTNAG	9 cut(s)	201, 236, 243, 255, 415, 440, 512, 578, 946
DpnI	GATC	3 cut(s)	213, 957, 1177
DpnII	GATC	3 cut(s)	211, 955, 1175
DraI	TTTAAA	1 cut(s)	900
DraIII	CACNNNGTG	1 cut(s)	71
Eam1104I	CTCTTC	3 cut(s)	37, 789, 1379
EarI	CTCTTC	3 cut(s)	37, 789, 1379
Ecl136II	GAGCTC	2 cut(s)	423, 1324
Eco130I	CCWWGG	2 cut(s)	538, 1276
Eco147I	AGGCCT	1 cut(s)	445
Eco24I	GRGCYC	3 cut(s)	425, 1326, 1546
Eco31I	GGTCTC	1 cut(s)	493
Eco47I	GGWCC	1 cut(s)	197
Eco53kI	GAGCTC	2 cut(s)	423, 1324
Eco81I	CCTNAGG	1 cut(s)	440
EcoICRI	GAGCTC	2 cut(s)	423, 1324
EcoT14I	CCWWGG	2 cut(s)	538, 1276
EcoT22I	ATGCAT	1 cut(s)	841
EcoT38I	GRGCYC	3 cut(s)	425, 1326, 1546
ErhI	CCWWGG	2 cut(s)	538, 1276
Esp3I	CGTCTC	1 cut(s)	918
FaqI	GGGAC	1 cut(s)	1348
FauI	CCCGC	2 cut(s)	410, 1043
FblI	GTMKAC	1 cut(s)	1587
Fnu4HI	GCNGC	2 cut(s)	391, 1094
FokI	GGATG	1 cut(s)	485
FriOI	GRGCYC	3 cut(s)	425, 1326, 1546
Fsp4HI	GCNGC	2 cut(s)	391, 1094
FspBI	CTAG	7 cut(s)	539, 633, 878, 917, 989, 1247, 1705
GlaI	GCGC	1 cut(s)	536
GluI	GCNGC	2 cut(s)	391, 1094
GsuI	CTGGAG	1 cut(s)	412
HaeII	RGCGCY	1 cut(s)	538
HaeIII	GGCC	4 cut(s)	445, 1161, 1275, 1389
HapII	CCGG	3 cut(s)	1032, 1138, 1583
HhaI	GCGC	1 cut(s)	537
Hin6I	GCGC	1 cut(s)	535
HinP1I	GCGC	1 cut(s)	535
HincII	GTYRAC	1 cut(s)	130
HindII	GTYRAC	1 cut(s)	130
HindIII	AAGCTT	1 cut(s)	822
HinfI	GANTC	3 cut(s)	29, 1452, 1676
HpaI	GTTAAC	1 cut(s)	130
HpaII	CCGG	3 cut(s)	1032, 1138, 1583
HphI	GGTGA	4 cut(s)	361, 470, 1471, 1561
Hpy166II	GTNNAC	8 cut(s)	130, 197, 434, 889, 1291, 1299, 1537, 1588
Hpy188III	TCNNGA	3 cut(s)	97, 686, 1577
Hpy8I	GTNNAC	8 cut(s)	130, 197, 434, 889, 1291, 1299, 1537, 1588
HpyAV	CCTTC	4 cut(s)	327, 601, 1107, 1354
HpyCH4III	ACNGT	4 cut(s)	69, 761, 831, 1667
HpyCH4IV	ACGT	4 cut(s)	593, 1380, 1435, 1644
HpyCH4V	TGCA	7 cut(s)	302, 839, 871, 1081, 1093, 1286, 1625
HpyF10VI	GCNNNNNNNGC	7 cut(s)	396, 411, 420, 532, 629, 1272, 1622
HpyF3I	CTNAG	9 cut(s)	201, 236, 243, 255, 415, 440, 512, 578, 946
HpySE526I	ACGT	4 cut(s)	593, 1380, 1435, 1644
HspAI	GCGC	1 cut(s)	535
KspAI	GTTAAC	1 cut(s)	130
Kzo9I	GATC	3 cut(s)	211, 955, 1175
LmnI	GCTCC	2 cut(s)	404, 428
Lsp1109I	GCAGC	1 cut(s)	1105
LweI	GCATC	3 cut(s)	463, 632, 848
MaeI	CTAG	7 cut(s)	539, 633, 878, 917, 989, 1247, 1705
MaeII	ACGT	4 cut(s)	593, 1380, 1435, 1644
MaeIII	GTNAC	1 cut(s)	367
MalI	GATC	3 cut(s)	213, 957, 1177
MbiI	CCGCTC	2 cut(s)	390, 405
MboI	GATC	3 cut(s)	211, 955, 1175
MboII	GAAGA	7 cut(s)	24, 71, 776, 990, 1303, 1366, 1672
MflI	RGATCY	1 cut(s)	1175
MhlI	GDGCHC	4 cut(s)	425, 658, 1326, 1546
MlyI	GAGTC	2 cut(s)	23, 1446
MmeI	TCCRAC	1 cut(s)	1210
Mph1103I	ATGCAT	1 cut(s)	841
MroXI	GAANNNNTTC	1 cut(s)	912
MseI	TTAA	5 cut(s)	129, 165, 480, 507, 899
MslI	CAYNNNNRTG	3 cut(s)	453, 482, 1281
MspI	CCGG	3 cut(s)	1032, 1138, 1583
MvnI	CGCG	1 cut(s)	1266
MwoI	GCNNNNNNNGC	7 cut(s)	396, 411, 420, 532, 629, 1272, 1622
NcoI	CCATGG	1 cut(s)	1276
NdeII	GATC	3 cut(s)	211, 955, 1175
NlaIV	GGNNCC	6 cut(s)	76, 106, 297, 499, 1177, 1498
NmuCI	GTSAC	1 cut(s)	367
NsiI	ATGCAT	1 cut(s)	841
NspI	RCATGY	1 cut(s)	877
PaqCI	CACCTGC	1 cut(s)	307
PceI	AGGCCT	1 cut(s)	445
PdmI	GAANNNNTTC	1 cut(s)	912
PfeI	GAWTC	1 cut(s)	1676
PflMI	CCANNNNNTGG	1 cut(s)	194
PinAI	ACCGGT	2 cut(s)	1031, 1582
PkrI	GCNGC	2 cut(s)	392, 1095
PleI	GAGTC	2 cut(s)	23, 1446
PpsI	GAGTC	2 cut(s)	23, 1446
Ppu21I	YACGTR	1 cut(s)	1436
PshAI	GACNNNNGTC	1 cut(s)	930
Psp124BI	GAGCTC	2 cut(s)	425, 1326
Psp1406I	AACGTT	1 cut(s)	1380
PspN4I	GGNNCC	6 cut(s)	76, 106, 297, 499, 1177, 1498
PspPI	GGNCC	2 cut(s)	197, 1273
PstI	CTGCAG	1 cut(s)	1627
PstNI	CAGNNNCTG	1 cut(s)	1396
PsuI	RGATCY	1 cut(s)	1175
RsaI	GTAC	6 cut(s)	241, 433, 758, 890, 1487, 1700
RsaNI	GTAC	6 cut(s)	240, 432, 757, 889, 1486, 1699
RseI	CAYNNNNRTG	3 cut(s)	453, 482, 1281
SacI	GAGCTC	2 cut(s)	425, 1326
SaqAI	TTAA	5 cut(s)	129, 165, 480, 507, 899
SatI	GCNGC	2 cut(s)	391, 1094
Sau3AI	GATC	3 cut(s)	211, 955, 1175
Sau96I	GGNCC	2 cut(s)	197, 1273
ScaI	AGTACT	3 cut(s)	241, 758, 1700
SchI	GAGTC	2 cut(s)	23, 1446
SduI	GDGCHC	4 cut(s)	425, 658, 1326, 1546
SfaNI	GCATC	3 cut(s)	463, 632, 848
SfcI	CTRYAG	4 cut(s)	46, 339, 760, 1623
SinI	GGWCC	1 cut(s)	197
SmiMI	CAYNNNNRTG	3 cut(s)	453, 482, 1281
SmlI	CTYRAG	2 cut(s)	530, 860
SmoI	CTYRAG	2 cut(s)	530, 860
SpeI	ACTAGT	1 cut(s)	988
SseBI	AGGCCT	1 cut(s)	445
SsiI	CCGC	5 cut(s)	390, 403, 466, 1050, 1266
SspMI	CTAG	7 cut(s)	539, 633, 878, 917, 989, 1247, 1705
SstI	GAGCTC	2 cut(s)	425, 1326
StuI	AGGCCT	1 cut(s)	445
StyI	CCWWGG	2 cut(s)	538, 1276
TaaI	ACNGT	4 cut(s)	69, 761, 831, 1667
TaiI	ACGT	4 cut(s)	596, 1383, 1438, 1647
TaqI	TCGA	4 cut(s)	116, 214, 954, 1527
TatI	WGTACW	5 cut(s)	239, 431, 756, 888, 1698
TauI	GCSGC	1 cut(s)	393
TfiI	GAWTC	1 cut(s)	1676
Tru1I	TTAA	5 cut(s)	129, 165, 480, 507, 899
Tru9I	TTAA	5 cut(s)	129, 165, 480, 507, 899
TscAI	CASTG	3 cut(s)	74, 565, 1293
TseFI	GTSAC	1 cut(s)	367
TseI	GCWGC	1 cut(s)	1093
Tsp45I	GTSAC	1 cut(s)	367
TspDTI	ATGAA	6 cut(s)	102, 365, 620, 990, 1303, 1668
TspGWI	ACGGA	1 cut(s)	956
TspRI	CASTG	3 cut(s)	74, 565, 1293
Van91I	CCANNNNNTGG	1 cut(s)	194
VpaK11BI	GGWCC	1 cut(s)	197
XapI	RAATTY	4 cut(s)	86, 304, 1400, 1690
XceI	RCATGY	1 cut(s)	877
XcmI	CCANNNNNNNNNTGG	2 cut(s)	610, 1016
XmaJI	CCTAGG	1 cut(s)	538
XmiI	GTMKAC	1 cut(s)	1587
XmnI	GAANNNNTTC	1 cut(s)	912
XspI	CTAG	7 cut(s)	539, 633, 878, 917, 989, 1247, 1705
ZrmI	AGTACT	3 cut(s)	241, 758, 1700
Zsp2I	ATGCAT	1 cut(s)	841

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.