Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004924

Physical Location & Seq

Genomic Coordinates Forward (+)

32948951 .. 32954628

Gene Structure 5678 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1233600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGGAGAGCGGGAAGATGGACAGGACCAGATTAGATTTAAGATTTCATCATCACTCTGGATCAACACAGTCTGAGGAGTCTGCTTTAGACTTGGAAAGGAATTACTGCAGTCATCATCCTAATCTACCTTCATTTAGTCCATCGGCCCTCCAGCCTTTTGCATCTAATGGTCAGCACTCTGAGAGCAATGCTGCCTACTTCTCATGGCCTACATTGGGTCGATTAAGTGATGCAGCAGAAGATAGAGCAAACTATTTTGGAAATCTTCAGAAGGAAGATCTTCCTGAAACTTTGGGCAGGTTGCCATCAGGGCAGCAGGCTACAACCTTACTTGAGCTGATGACCATCAGGGCATTCCATAGCAAGATATTGCGGCTTTTTAGTCTTGGCACAGCAATTGGGTTTCGGATTCGAAAGGGTGTCTTAACAGACATTCCGGCCATTCTTGTCTTTGTTGCCCGTAAAGCTCACAGGGAATGGCTCAGCCATGTGCAGTGTCTACCCGCTGCCCTTGAGGGGCCAGGAGGTGTATGGTGTGATGTGGATGTTGTTGAATTTTCCTATTATGGCTCCCCGGTTTCAACTCCTAAAGAACAGCTGTACACAGAGCTCGCAGATGGCTTGAGGGGAAGCGATCCATACATTGGTTCTGGTTCCCAGGTTGCTAGCCAGGATACGTATGGAACTTTGGGTGCTATTGTTAAAAGCCGAACAGGAAATCGACAGGTTGGTTTTCTCACAAATCGGCATGTGGCAGTTGATTTGGACTATCCAAACCAGAAAATGTTTCATCCATTGCCACCAAGCCTTGGACCTGGGGTGTATCTTGGCGCTGTGGAGAGGGCAACATCTTTTATCACAGATGATCTTTGGTACGGTATATTTGCTGGAACAAACCCAGAAACATTTGTGCGAGCTGATGGTGCCTTCATTCCTTTTGCCGAAGATTTCAACTTGAAAAATGTAATTACAACTGTAAGAGGCATAGGTGAGATTGGTGATGTCTACACCATAGACTTGCAGTCTCCAATCAACAGTCTTATCGGAAGGCAAGTGATGAAAGTTGGGAGAAGTTCAGGCTTGACGACTGGGACCATCATGGCGTATGCCCTAGAATACAATGATGAAAAAGGGATATGTTTCTTTACTGATTTTCTTGTTGTTGGAGAGAATCAGCAGAGTTTTGATCTTGAAGGCGATAGTGGAAGCCTCATTCTATTGACTGGTCAGAATGGGGAGAAGCCACGACCGGTTGGGATCATTTGGGGCGGGACCGCTAACCGTGGTCGCTTGAAACTAAAAATTGGCCAACCACCGGAAAATTGGACTAGTGGAGTTGATCTTGGACGCCTTCTTCAACTCCTTGAACTTGACCTGATAACAACCAAGGAAGGGTTTCAAGCTGCTATCCAAGAGCAACGAAATGCTGCTGCGGGGATTGGTTCCACAGTTGGGGAGTCATCCCCAGCTGTTCGGCCCCCTTGTAAAGATAAGCTAGAAGATAACTTTGAACCTTTGGGTTTCAATCTTCAGAAAATTCCAATTGAAGGTGAGTCCTGTCAGGGACTGATTCGACCATTCATGCGCGGTGACTTTCACATAGAAAATTGTGTTGAAACTGCTCCAAATATAGAACACCAATTCATTCCCAGTGCCACCAGCAGATCTCCAGGAAATCAAAACAATCAAGAGGGAAATCCGGTACCAAAAAACCTTTTGCCATTGCAGAGTAGCTTGGATGAAGAGATATCTGTTTCGTTGCAGTTAGGTGAGCCTGAACCGAAGAGAAGAAAAAACTGCTACTCTCTCTTTAGCAGTAAAGGACCCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

611

Amino Acids

66.77

Weight (kDa)

5.44

Isoelectric Point (pI)

42.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAL1_N	PF25608	105 - 184	1e-42	NARROW LEAF 1 N-terminal domain
Nal1_C	PF25819	197 - 464	1e-158	Nal1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004924)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35155 AT2G35155 AT5G45030 AT5G45030
fragaria_vesca	FvH4_3g12240 FvH4_3g12240 FvH4_3g12240 FvH4_3g12240 FvH4_3g12240 FvH4_3g12240
malus_domestica	MD05G1252600.v1.1 MD10G1233600.v1.1
prunus_persica	Prupe.4G111500_v2.0.a1 Prupe.4G111500_v2.0.a1 Prupe.4G111500_v2.0.a1 Prupe.4G111500_v2.0.a1
pyrus_communis	pycom10g19590
rosa_chinensis	RchiOBHm_Chr5g0020051
rosa_laevigata	RLG00000032494 RLG00000032496
rosa_multiflora	Rmu_sc0002250.1_g000001 Rmu_sc0009586.1_g000004
rosa_roxburghii	Rroxscaffold_1G00058150
rosa_rugosa	Rorug05G0056400 Rorug05G0056500
rosa_samantha	Rh5AG146600 Rh5BG146100 Rh5CG157600 Rh5DG145500
rosa_wichuraiana	Rw5G012940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	288
Acc65I	GGTACC	1 cut(s)	1702
AccB1I	GGYRCC	2 cut(s)	923, 1702
AccB7I	CCANNNNNTGG	2 cut(s)	644, 809
AccBSI	CCGCTC	1 cut(s)	9
AccI	GTMKAC	2 cut(s)	499, 1005
AccII	CGCG	1 cut(s)	1587
AciI	CCGC	7 cut(s)	9, 373, 504, 1269, 1275, 1433, 1587
AclWI	GGATC	3 cut(s)	67, 629, 1265
AcoI	YGGCCR	2 cut(s)	438, 1306
AcsI	RAATTY	2 cut(s)	554, 1536
AcuI	CTGAAG	2 cut(s)	251, 1514
AcyI	GRCGYC	1 cut(s)	1348
AfaI	GTAC	3 cut(s)	602, 875, 1704
AfiI	CCNNNNNNNGG	8 cut(s)	215, 516, 644, 809, 1315, 1392, 1452, 1547
AgeI	ACCGGT	1 cut(s)	1249
AhdI	GACNNNNNGTC	1 cut(s)	1021
AhlI	ACTAGT	1 cut(s)	1328
AjnI	CCWGG	5 cut(s)	520, 657, 669, 814, 1669
AluBI	AGCT	9 cut(s)	337, 467, 598, 610, 917, 1403, 1469, 1495, 1734
AluI	AGCT	9 cut(s)	337, 467, 598, 610, 917, 1403, 1469, 1495, 1734
Alw21I	GWGCWC	1 cut(s)	612
Alw26I	GTCTC	1 cut(s)	1029
AlwI	GGATC	3 cut(s)	67, 629, 1265
AlwNI	CAGNNNCTG	1 cut(s)	1567
AoxI	GGCC	6 cut(s)	144, 206, 438, 518, 1306, 1475
ApeKI	GCWGC	7 cut(s)	191, 233, 313, 506, 1403, 1427, 1430
ApoI	RAATTY	2 cut(s)	554, 1536
AsiGI	ACCGGT	1 cut(s)	1249
Asp700I	GAANNNNTTC	2 cut(s)	279, 1395
Asp718I	GGTACC	1 cut(s)	1702
AspLEI	GCGC	2 cut(s)	833, 1587
AspS9I	GGNCC	8 cut(s)	24, 145, 518, 812, 1092, 1272, 1476, 1823
AsuC2I	CCSGG	1 cut(s)	575
AsuHPI	GGTGA	5 cut(s)	1001, 1010, 1562, 1601, 1781
AsuII	TTCGAA	1 cut(s)	412
AsuNHI	GCTAGC	1 cut(s)	665
AvaII	GGWCC	5 cut(s)	24, 812, 1092, 1272, 1823
BalI	TGGCCA	1 cut(s)	1308
BanI	GGYRCC	2 cut(s)	923, 1702
BanII	GRGCYC	1 cut(s)	612
Bbv12I	GWGCWC	1 cut(s)	612
BbvI	GCAGC	7 cut(s)	178, 245, 325, 493, 1390, 1414, 1417
BccI	CCATC	7 cut(s)	10, 148, 313, 353, 611, 914, 1103
BciT130I	CCWGG	5 cut(s)	522, 659, 671, 816, 1671
BciVI	GTATCC	1 cut(s)	667
BcnI	CCSGG	1 cut(s)	575
BcoDI	GTCTC	1 cut(s)	1029
BcuI	ACTAGT	1 cut(s)	1328
BfaI	CTAG	4 cut(s)	666, 1112, 1329, 1496
BfmI	CTRYAG	1 cut(s)	106
BfoI	RGCGCY	1 cut(s)	834
BfuAI	ACCTGC	1 cut(s)	288
BfuI	GTATCC	1 cut(s)	667
BglI	GCCNNNNNGGC	1 cut(s)	310
BglII	AGATCT	2 cut(s)	277, 1664
BisI	GCNGC	8 cut(s)	192, 234, 314, 374, 507, 1404, 1428, 1431
BlpI	GCTNAGC	1 cut(s)	482
BlsI	GCNGC	8 cut(s)	193, 235, 315, 375, 508, 1405, 1429, 1432
Bme1390I	CCNGG	6 cut(s)	522, 575, 659, 671, 816, 1671
Bme18I	GGWCC	5 cut(s)	24, 812, 1092, 1272, 1823
BmeRI	GACNNNNNGTC	1 cut(s)	1021
BmgT120I	GGNCC	8 cut(s)	24, 145, 518, 812, 1092, 1272, 1476, 1823
BmrFI	CCNGG	6 cut(s)	522, 575, 659, 671, 816, 1671
BmrI	ACTGGG	2 cut(s)	1098, 1644
BmsI	GCATC	2 cut(s)	170, 220
BmtI	GCTAGC	1 cut(s)	669
BmuI	ACTGGG	2 cut(s)	1098, 1644
BpmI	CTGGAG	2 cut(s)	134, 1653
Bpu1102I	GCTNAGC	1 cut(s)	482
Bpu14I	TTCGAA	1 cut(s)	412
BpuEI	CTTGAG	3 cut(s)	353, 533, 643
BpuMI	CCSGG	1 cut(s)	575
BsaAI	YACGTR	1 cut(s)	678
BsaBI	GATNNNNATC	1 cut(s)	344
BsaHI	GRCGYC	1 cut(s)	1348
BsaJI	CCNNGG	6 cut(s)	573, 657, 808, 815, 1282, 1386
BsaWI	WCCGGW	3 cut(s)	1249, 1315, 1699
Bsc4I	CCNNNNNNNGG	8 cut(s)	215, 516, 644, 809, 1315, 1392, 1452, 1547
Bse118I	RCCGGY	1 cut(s)	1249
Bse1I	ACTGG	3 cut(s)	1093, 1228, 1650
Bse3DI	GCAATG	3 cut(s)	193, 794, 1721
Bse8I	GATNNNNATC	1 cut(s)	344
BseBI	CCWGG	5 cut(s)	522, 659, 671, 816, 1671
BseDI	CCNNGG	6 cut(s)	573, 657, 808, 815, 1282, 1386
BseGI	GGATG	5 cut(s)	115, 550, 790, 1460, 1744
BseJI	GATNNNNATC	1 cut(s)	344
BseLI	CCNNNNNNNGG	8 cut(s)	215, 516, 644, 809, 1315, 1392, 1452, 1547
BseMI	GCAATG	3 cut(s)	193, 794, 1721
BseMII	CTCAG	3 cut(s)	63, 171, 496
BseNI	ACTGG	3 cut(s)	1093, 1228, 1650
BseRI	GAGGAG	1 cut(s)	89
BseXI	GCAGC	7 cut(s)	178, 245, 325, 493, 1390, 1414, 1417
BseYI	CCCAGC	1 cut(s)	1465
BsgI	GTGCAG	1 cut(s)	512
Bsh1236I	CGCG	1 cut(s)	1587
Bsh1285I	CGRYCG	1 cut(s)	1250
BshFI	GGCC	6 cut(s)	146, 208, 440, 520, 1308, 1477
BshNI	GGYRCC	2 cut(s)	923, 1702
BshTI	ACCGGT	1 cut(s)	1249
BsiEI	CGRYCG	1 cut(s)	1250
BsiHKAI	GWGCWC	1 cut(s)	612
BsiSI	CCGG	5 cut(s)	437, 575, 1250, 1316, 1700
BslFI	GGGAC	3 cut(s)	1105, 1285, 1578
BslI	CCNNNNNNNGG	8 cut(s)	215, 516, 644, 809, 1315, 1392, 1452, 1547
BsmAI	GTCTC	1 cut(s)	1029
BsmFI	GGGAC	3 cut(s)	1105, 1285, 1578
BsmI	GAATGC	1 cut(s)	353
BsnI	GGCC	6 cut(s)	146, 208, 440, 520, 1308, 1477
Bsp119I	TTCGAA	1 cut(s)	412
Bsp1286I	GDGCHC	1 cut(s)	612
Bsp1407I	TGTACA	1 cut(s)	600
Bsp143I	GATC	8 cut(s)	59, 277, 634, 865, 1186, 1257, 1339, 1664
Bsp1720I	GCTNAGC	1 cut(s)	482
BspACI	CCGC	7 cut(s)	9, 373, 504, 1269, 1275, 1433, 1587
BspANI	GGCC	6 cut(s)	146, 208, 440, 520, 1308, 1477
BspCNI	CTCAG	3 cut(s)	64, 172, 495
BspFNI	CGCG	1 cut(s)	1587
BspMAI	CTGCAG	1 cut(s)	110
BspMI	ACCTGC	1 cut(s)	288
BspOI	GCTAGC	1 cut(s)	669
BspPI	GGATC	3 cut(s)	67, 629, 1265
BspT104I	TTCGAA	1 cut(s)	412
BspT107I	GGYRCC	2 cut(s)	923, 1702
BsrBI	CCGCTC	1 cut(s)	9
BsrDI	GCAATG	3 cut(s)	193, 794, 1721
BsrFI	RCCGGY	1 cut(s)	1249
BsrGI	TGTACA	1 cut(s)	600
BsrI	ACTGG	3 cut(s)	1093, 1228, 1650
BssAI	RCCGGY	1 cut(s)	1249
BssECI	CCNNGG	6 cut(s)	573, 657, 808, 815, 1282, 1386
BssMI	GATC	8 cut(s)	59, 277, 634, 865, 1186, 1257, 1339, 1664
BssNI	GRCGYC	1 cut(s)	1348
BssT1I	CCWWGG	2 cut(s)	808, 1386
Bst2UI	CCWGG	5 cut(s)	522, 659, 671, 816, 1671
Bst4CI	ACNGT	6 cut(s)	69, 878, 976, 1037, 1283, 1450
Bst6I	CTCTTC	2 cut(s)	1737, 1778
BstACI	GRCGYC	1 cut(s)	1348
BstAUI	TGTACA	1 cut(s)	600
BstBAI	YACGTR	1 cut(s)	678
BstBI	TTCGAA	1 cut(s)	412
BstC8I	GCNNGC	4 cut(s)	318, 612, 667, 915
BstDEI	CTNAG	3 cut(s)	72, 180, 482
BstDSI	CCRYGG	1 cut(s)	1282
BstF5I	GGATG	5 cut(s)	115, 550, 790, 1460, 1744
BstFNI	CGCG	1 cut(s)	1587
BstH2I	RGCGCY	1 cut(s)	834
BstHHI	GCGC	2 cut(s)	833, 1587
BstKTI	GATC	8 cut(s)	62, 280, 637, 868, 1189, 1260, 1342, 1667
BstMAI	GTCTC	1 cut(s)	1029
BstMBI	GATC	8 cut(s)	59, 277, 634, 865, 1186, 1257, 1339, 1664
BstMCI	CGRYCG	1 cut(s)	1250
BstMWI	GCNNNNNNNGC	3 cut(s)	310, 464, 923
BstNI	CCWGG	5 cut(s)	522, 659, 671, 816, 1671
BstNSI	RCATGY	1 cut(s)	752
BstSCI	CCNGG	6 cut(s)	520, 573, 657, 669, 814, 1669
BstSFI	CTRYAG	1 cut(s)	106
BstSNI	TACGTA	1 cut(s)	678
BstUI	CGCG	1 cut(s)	1587
BstV1I	GCAGC	7 cut(s)	178, 245, 325, 493, 1390, 1414, 1417
BstX2I	RGATCY	2 cut(s)	277, 1664
BstYI	RGATCY	2 cut(s)	277, 1664
BsuI	GTATCC	1 cut(s)	667
BsuRI	GGCC	6 cut(s)	146, 208, 440, 520, 1308, 1477
BtgI	CCRYGG	1 cut(s)	1282
BtsCI	GGATG	5 cut(s)	115, 550, 790, 1460, 1744
BtsI	GCAGTG	1 cut(s)	500
BtsIMutI	CAGTG	2 cut(s)	500, 1657
BveI	ACCTGC	1 cut(s)	288
Cac8I	GCNNGC	4 cut(s)	318, 612, 667, 915
CaiI	CAGNNNCTG	1 cut(s)	1567
CfoI	GCGC	2 cut(s)	833, 1587
Cfr10I	RCCGGY	1 cut(s)	1249
Cfr13I	GGNCC	8 cut(s)	24, 145, 518, 812, 1092, 1272, 1476, 1823
CseI	GACGC	1 cut(s)	1356
Csp6I	GTAC	3 cut(s)	601, 874, 1703
CspAI	ACCGGT	1 cut(s)	1249
CviAII	CATG	5 cut(s)	204, 488, 749, 1099, 1582
CviQI	GTAC	3 cut(s)	601, 874, 1703
DdeI	CTNAG	3 cut(s)	72, 180, 482
DpnI	GATC	8 cut(s)	61, 279, 636, 867, 1188, 1259, 1341, 1666
DpnII	GATC	8 cut(s)	59, 277, 634, 865, 1186, 1257, 1339, 1664
DriI	GACNNNNNGTC	1 cut(s)	1021
EaeI	YGGCCR	2 cut(s)	438, 1306
Eam1104I	CTCTTC	2 cut(s)	1737, 1778
Eam1105I	GACNNNNNGTC	1 cut(s)	1021
EarI	CTCTTC	2 cut(s)	1737, 1778
Ecl136II	GAGCTC	1 cut(s)	610
Eco105I	TACGTA	1 cut(s)	678
Eco130I	CCWWGG	2 cut(s)	808, 1386
Eco24I	GRGCYC	1 cut(s)	612
Eco32I	GATATC	1 cut(s)	1749
Eco47I	GGWCC	5 cut(s)	24, 812, 1092, 1272, 1823
Eco53kI	GAGCTC	1 cut(s)	610
Eco57I	CTGAAG	2 cut(s)	251, 1514
EcoICRI	GAGCTC	1 cut(s)	610
EcoO109I	RGGNCCY	1 cut(s)	1823
EcoRII	CCWGG	5 cut(s)	520, 657, 669, 814, 1669
EcoRV	GATATC	1 cut(s)	1749
EcoT14I	CCWWGG	2 cut(s)	808, 1386
EcoT38I	GRGCYC	1 cut(s)	612
ErhI	CCWWGG	2 cut(s)	808, 1386
FaeI	CATG	5 cut(s)	207, 491, 752, 1102, 1585
FalI	AAGNNNNNCTT	2 cut(s)	407, 439
FaqI	GGGAC	3 cut(s)	1105, 1285, 1578
FatI	CATG	5 cut(s)	203, 487, 748, 1098, 1581
FauI	CCCGC	4 cut(s)	2, 511, 1262, 1426
FauNDI	CATATG	1 cut(s)	1829
FblI	GTMKAC	2 cut(s)	499, 1005
Fnu4HI	GCNGC	8 cut(s)	192, 234, 314, 374, 507, 1404, 1428, 1431
FokI	GGATG	5 cut(s)	102, 557, 777, 1447, 1751
FriOI	GRGCYC	1 cut(s)	612
Fsp4HI	GCNGC	8 cut(s)	192, 234, 314, 374, 507, 1404, 1428, 1431
FspBI	CTAG	4 cut(s)	666, 1112, 1329, 1496
GlaI	GCGC	2 cut(s)	832, 1586
GluI	GCNGC	8 cut(s)	192, 234, 314, 374, 507, 1404, 1428, 1431
GsaI	CCCAGC	1 cut(s)	1469
GsuI	CTGGAG	2 cut(s)	134, 1653
HaeII	RGCGCY	1 cut(s)	834
HaeIII	GGCC	6 cut(s)	146, 208, 440, 520, 1308, 1477
HapII	CCGG	5 cut(s)	437, 575, 1250, 1316, 1700
HgaI	GACGC	1 cut(s)	1356
HhaI	GCGC	2 cut(s)	833, 1587
Hin1I	GRCGYC	1 cut(s)	1348
Hin1II	CATG	5 cut(s)	207, 491, 752, 1102, 1585
Hin6I	GCGC	2 cut(s)	831, 1585
HinP1I	GCGC	2 cut(s)	831, 1585
HinfI	GANTC	6 cut(s)	77, 409, 1171, 1457, 1553, 1570
HpaII	CCGG	5 cut(s)	437, 575, 1250, 1316, 1700
HphI	GGTGA	5 cut(s)	1001, 1010, 1562, 1601, 1781
Hpy166II	GTNNAC	3 cut(s)	500, 603, 1006
Hpy188I	TCNGA	7 cut(s)	73, 181, 270, 408, 1046, 1230, 1533
Hpy188III	TCNNGA	4 cut(s)	57, 284, 1190, 1688
Hpy8I	GTNNAC	3 cut(s)	500, 603, 1006
HpyAV	CCTTC	8 cut(s)	138, 265, 937, 1041, 1187, 1361, 1385, 1541
HpyCH4III	ACNGT	6 cut(s)	69, 878, 976, 1037, 1283, 1450
HpyCH4IV	ACGT	1 cut(s)	677
HpyCH4V	TGCA	7 cut(s)	108, 161, 233, 493, 1021, 1726, 1762
HpyF10VI	GCNNNNNNNGC	3 cut(s)	310, 464, 923
HpyF3I	CTNAG	3 cut(s)	72, 180, 482
HpySE526I	ACGT	1 cut(s)	677
Hsp92I	GRCGYC	1 cut(s)	1348
Hsp92II	CATG	5 cut(s)	207, 491, 752, 1102, 1585
HspAI	GCGC	2 cut(s)	831, 1585
KpnI	GGTACC	1 cut(s)	1706
Kzo9I	GATC	8 cut(s)	59, 277, 634, 865, 1186, 1257, 1339, 1664
LmnI	GCTCC	2 cut(s)	575, 1627
Lsp1109I	GCAGC	7 cut(s)	178, 245, 325, 493, 1390, 1414, 1417
LweI	GCATC	2 cut(s)	170, 220
MaeI	CTAG	4 cut(s)	666, 1112, 1329, 1496
MaeII	ACGT	1 cut(s)	677
MaeIII	GTNAC	1 cut(s)	1589
MalI	GATC	8 cut(s)	61, 279, 636, 867, 1188, 1259, 1341, 1666
MbiI	CCGCTC	1 cut(s)	9
MboI	GATC	8 cut(s)	59, 277, 634, 865, 1186, 1257, 1339, 1664
MfeI	CAATTG	2 cut(s)	396, 1542
MflI	RGATCY	2 cut(s)	277, 1664
MhlI	GDGCHC	1 cut(s)	612
MlsI	TGGCCA	1 cut(s)	1308
MluNI	TGGCCA	1 cut(s)	1308
MlyI	GAGTC	3 cut(s)	86, 1466, 1562
MmeI	TCCRAC	1 cut(s)	1144
MnlI	CCTC	9 cut(s)	67, 158, 508, 518, 618, 834, 974, 1220, 1684
Mox20I	TGGCCA	1 cut(s)	1308
MroXI	GAANNNNTTC	2 cut(s)	279, 1395
MscI	TGGCCA	1 cut(s)	1308
MseI	TTAA	4 cut(s)	38, 224, 425, 702
Msp20I	TGGCCA	1 cut(s)	1308
MspA1I	CMGCKG	3 cut(s)	506, 598, 1469
MspI	CCGG	5 cut(s)	437, 575, 1250, 1316, 1700
MspR9I	CCNGG	6 cut(s)	522, 575, 659, 671, 816, 1671
MunI	CAATTG	2 cut(s)	396, 1542
Mva1269I	GAATGC	1 cut(s)	353
MvaI	CCWGG	5 cut(s)	522, 659, 671, 816, 1671
MvnI	CGCG	1 cut(s)	1587
MwoI	GCNNNNNNNGC	3 cut(s)	310, 464, 923
NciI	CCSGG	1 cut(s)	575
NdeI	CATATG	1 cut(s)	1829
NdeII	GATC	8 cut(s)	59, 277, 634, 865, 1186, 1257, 1339, 1664
NheI	GCTAGC	1 cut(s)	665
NlaIII	CATG	5 cut(s)	207, 491, 752, 1102, 1585
NmuCI	GTSAC	1 cut(s)	1589
NspI	RCATGY	1 cut(s)	752
NspV	TTCGAA	1 cut(s)	412
PctI	GAATGC	1 cut(s)	353
PdmI	GAANNNNTTC	2 cut(s)	279, 1395
PfeI	GAWTC	3 cut(s)	409, 1171, 1570
PflMI	CCANNNNNTGG	2 cut(s)	644, 809
PfoI	TCCNGGA	1 cut(s)	1669
PinAI	ACCGGT	1 cut(s)	1249
PkrI	GCNGC	8 cut(s)	193, 235, 315, 375, 508, 1405, 1429, 1432
PleI	GAGTC	3 cut(s)	85, 1465, 1561
PpsI	GAGTC	3 cut(s)	85, 1465, 1561
Ppu21I	YACGTR	1 cut(s)	678
PpuMI	RGGWCCY	1 cut(s)	1823
Psp124BI	GAGCTC	1 cut(s)	612
Psp5II	RGGWCCY	1 cut(s)	1823
Psp6I	CCWGG	5 cut(s)	520, 657, 669, 814, 1669
PspFI	CCCAGC	1 cut(s)	1465
PspGI	CCWGG	5 cut(s)	520, 657, 669, 814, 1669
PspPI	GGNCC	8 cut(s)	24, 145, 518, 812, 1092, 1272, 1476, 1823
PspPPI	RGGWCCY	1 cut(s)	1823
PstI	CTGCAG	1 cut(s)	110
PstNI	CAGNNNCTG	1 cut(s)	1567
PsuI	RGATCY	2 cut(s)	277, 1664
PvuII	CAGCTG	2 cut(s)	598, 1469
RsaI	GTAC	3 cut(s)	602, 875, 1704
RsaNI	GTAC	3 cut(s)	601, 874, 1703
SacI	GAGCTC	1 cut(s)	612
SaqAI	TTAA	4 cut(s)	38, 224, 425, 702
SatI	GCNGC	8 cut(s)	192, 234, 314, 374, 507, 1404, 1428, 1431
Sau3AI	GATC	8 cut(s)	59, 277, 634, 865, 1186, 1257, 1339, 1664
Sau96I	GGNCC	8 cut(s)	24, 145, 518, 812, 1092, 1272, 1476, 1823
SchI	GAGTC	3 cut(s)	86, 1466, 1562
ScrFI	CCNGG	6 cut(s)	522, 575, 659, 671, 816, 1671
SduI	GDGCHC	1 cut(s)	612
SfaNI	GCATC	2 cut(s)	170, 220
SfcI	CTRYAG	1 cut(s)	106
SfuI	TTCGAA	1 cut(s)	412
SinI	GGWCC	5 cut(s)	24, 812, 1092, 1272, 1823
SmlI	CTYRAG	3 cut(s)	332, 512, 622
SmoI	CTYRAG	3 cut(s)	332, 512, 622
SnaBI	TACGTA	1 cut(s)	678
SpeI	ACTAGT	1 cut(s)	1328
SsiI	CCGC	7 cut(s)	9, 373, 504, 1269, 1275, 1433, 1587
SspMI	CTAG	4 cut(s)	666, 1112, 1329, 1496
SstI	GAGCTC	1 cut(s)	612
StyD4I	CCNGG	6 cut(s)	520, 573, 657, 669, 814, 1669
StyI	CCWWGG	2 cut(s)	808, 1386
TaaI	ACNGT	6 cut(s)	69, 878, 976, 1037, 1283, 1450
TaiI	ACGT	1 cut(s)	680
TaqI	TCGA	4 cut(s)	220, 412, 721, 1573
TatI	WGTACW	1 cut(s)	600
TauI	GCSGC	1 cut(s)	376
TfiI	GAWTC	3 cut(s)	409, 1171, 1570
Tru1I	TTAA	4 cut(s)	38, 224, 425, 702
Tru9I	TTAA	4 cut(s)	38, 224, 425, 702
TscAI	CASTG	2 cut(s)	500, 1657
TseFI	GTSAC	1 cut(s)	1589
TseI	GCWGC	7 cut(s)	191, 233, 313, 506, 1403, 1427, 1430
Tsp45I	GTSAC	1 cut(s)	1589
TspDTI	ATGAA	9 cut(s)	35, 120, 779, 919, 1073, 1140, 1570, 1633, 1755
TspRI	CASTG	2 cut(s)	500, 1657
Van91I	CCANNNNNTGG	2 cut(s)	644, 809
VpaK11BI	GGWCC	5 cut(s)	24, 812, 1092, 1272, 1823
XapI	RAATTY	2 cut(s)	554, 1536
XceI	RCATGY	1 cut(s)	752
XcmI	CCANNNNNNNNNTGG	3 cut(s)	528, 677, 1320
XmiI	GTMKAC	2 cut(s)	499, 1005
XmnI	GAANNNNTTC	2 cut(s)	279, 1395
XspI	CTAG	4 cut(s)	666, 1112, 1329, 1496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.