MD10G1292100.v1.1

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011355
Physical Location & Seq
Forward (+)
38045551 .. 38046887
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1292100.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGTTGAGCAACTGTGATCATGAGAAGATGGCTCTACTCATCTCTTCAACTACTAACGAATGGCCACAGATGCAGCAGGATATTCAAATCGATGATATAGATCATCAAAGGGGCTTGAACAACAACAATAACGCTATGTCTTCATCGACGTCGTCGTCGGCGAATCGGATAATGGAGAAACCAGGTCAAGAACAACTCCAGCTCCAACAGCAAGCCCTAAAGTGCCCTCGCTGCGATTCGTCAAACACCAAGTTCTGTTACTACAACAACTACAGCTTGTCTCAGCCGAGGCACTTCTGCAAGGCCTGCAAGCGCTACTGGACCCGAGGTGGGACCCTCCGCAACGTTCCAGTCGGTGGAGGATGCCGAAAGAACAAGCGCGTGAAGAGGCCTGGCTCGACATCTTCAACATCATCAGCCATGGATGGTGGAACTCCGTCTTCTTCCTCTTCAGTTCCTAGCACTACTGCTGCTGTTAATAATAATAATTCAAACCCTAATTCACACCCGTCCCACATCGATCAAATCAATCCGCTCTTTTATGGGTTGTCTAGTAACCCTTTTATGGATACTGCTTCAGGGTTTGATCTTCATCAGTTGAATCATCAGGCTCAGCTGGGATTAGGGTTTTCATCATCGTCAGGTACTAATATGATGTCCAGTGATAATTATCGAAACCGGTTTAATCCCGCTTCAAATTCATCGCTTCCTTCCAATTATAGTTCCATGTTTAGTACTTCCCCATCCACCACAGCTCCAACTGTGGCTTCTGTGCTTGCTTCTAAATTCATCAATGGCGGTGCTTTCAAAGATATTCAAACTAGAGGGCCTCATCAGCATAATCAATACTTCCAGTCCTTGCTACCATTTGAAGATCTTCAAATAGCGGCCGGAAGCAACGGTGAGGCTGGTAGTGCCTCGAAAGACGTGAAAGTAGAAGATCATGGTGGCCAAAACAGGTTAGAGTGGAATAATAACAATGGCAGCAGTAATGTGAATGGTAATATGAATTGCCAGAATCAGATCACGGAGCAGATGGGGCTCTCAGATCCTACTCTTTACTGGAACTCATCGACTCTCGGTTCTAATTGGCATGATCCGGCAAATCTTGGGTCCTCAGTCTCTTCACTGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-Dof

Protein Analysis

379

Amino Acids

41.07

Weight (kDa)

7.64

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 71 - 127 5.7e-34 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011355)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28310 AT1G28310
fragaria_vesca FvH4_3g08890 FvH4_3g08890
malus_domestica MD05G1312400.v1.1 MD10G1292100.v1.1
prunus_persica Prupe.4G050300_v2.0.a1
pyrus_communis pycom05g28840 pycom10g24410
rosa_chinensis RchiOBHm_Chr5g0008861
rosa_laevigata RLG00000031610
rosa_multiflora Rmu_sc0004677.1_g000005
rosa_roxburghii Rroxscaffold_1G00067140 Rroxscaffold_1G00067760
rosa_rugosa Rorug04G0439800
rosa_samantha Rh5AG068300 Rh5BG065100 Rh5CG076300 Rh5DG060600
rosa_wichuraiana Rw5G006400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 154
AatII GACGTC 1 cut(s) 152
AccB7I CCANNNNNTGG 1 cut(s) 356
AccBSI CCGCTC 1 cut(s) 535
AccII CGCG 1 cut(s) 381
AciI CCGC 5 cut(s) 340, 533, 690, 798, 887
AclI AACGTT 1 cut(s) 345
AclWI GGATC 2 cut(s) 1043, 1091
AcoI YGGCCR 3 cut(s) 62, 888, 949
AcsI RAATTY 2 cut(s) 697, 785
AcuI CTGAAG 2 cut(s) 435, 561
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 2 cut(s) 646, 736
AfeI AGCGCT 1 cut(s) 314
AfiI CCNNNNNNNGG 3 cut(s) 330, 356, 565
AgeI ACCGGT 1 cut(s) 678
AjiI CACGTC 1 cut(s) 928
AjnI CCWGG 2 cut(s) 181, 391
AjuI GAANNNNNNNTTGG 2 cut(s) 751, 783
AluBI AGCT 4 cut(s) 202, 276, 616, 755
AluI AGCT 4 cut(s) 202, 276, 616, 755
Alw26I GTCTC 2 cut(s) 285, 1126
AlwI GGATC 2 cut(s) 1043, 1091
Ama87I CYCGRG 1 cut(s) 324
Aor51HI AGCGCT 1 cut(s) 314
AoxI GGCC 6 cut(s) 62, 303, 389, 827, 888, 949
ApeKI GCWGC 4 cut(s) 73, 231, 470, 984
ApoI RAATTY 2 cut(s) 697, 785
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
AsiGI ACCGGT 1 cut(s) 678
Asp700I GAANNNNTTC 1 cut(s) 876
AspLEI GCGC 2 cut(s) 315, 381
AspS9I GGNCC 4 cut(s) 321, 333, 827, 1113
AsuHPI GGTGA 1 cut(s) 914
AvaI CYCGRG 1 cut(s) 324
AvaII GGWCC 3 cut(s) 321, 333, 1113
BaeGI GKGCMC 1 cut(s) 227
BalI TGGCCA 2 cut(s) 64, 951
BanII GRGCYC 1 cut(s) 1044
BbsI GAAGAC 2 cut(s) 132, 432
BbvI GCAGC 4 cut(s) 85, 218, 457, 996
BccI CCATC 4 cut(s) 22, 419, 751, 1030
BciT130I CCWGG 2 cut(s) 183, 393
BciVI GTATCC 1 cut(s) 562
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 2 cut(s) 285, 1126
BfaI CTAG 4 cut(s) 459, 552, 822, 1135
BfmI CTRYAG 1 cut(s) 271
BfoI RGCGCY 1 cut(s) 316
BfuI GTATCC 1 cut(s) 562
BglII AGATCT 1 cut(s) 874
BisI GCNGC 5 cut(s) 74, 232, 471, 888, 985
BlpI GCTNAGC 1 cut(s) 612
BlsI GCNGC 5 cut(s) 75, 233, 472, 889, 986
BmcAI AGTACT 1 cut(s) 736
Bme1390I CCNGG 2 cut(s) 183, 393
Bme18I GGWCC 3 cut(s) 321, 333, 1113
BmeT110I CYCGRG 1 cut(s) 324
BmgBI CACGTC 1 cut(s) 928
BmgT120I GGNCC 4 cut(s) 321, 333, 827, 1113
BmiI GGNNCC 4 cut(s) 323, 334, 335, 1114
BmrFI CCNGG 2 cut(s) 183, 393
BmsI GCATC 2 cut(s) 60, 353
BpiI GAAGAC 2 cut(s) 132, 432
BpmI CTGGAG 1 cut(s) 182
Bpu1102I GCTNAGC 1 cut(s) 612
Bsa29I ATCGAT 2 cut(s) 90, 519
BsaBI GATNNNNATC 3 cut(s) 99, 591, 669
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 3 cut(s) 287, 325, 420
BsaWI WCCGGW 1 cut(s) 678
BsaXI ACNNNNNCTCC 4 cut(s) 186, 216, 739, 769
Bsc4I CCNNNNNNNGG 3 cut(s) 330, 356, 565
Bse118I RCCGGY 1 cut(s) 678
Bse1I ACTGG 5 cut(s) 323, 350, 660, 853, 1067
Bse8I GATNNNNATC 3 cut(s) 99, 591, 669
BseBI CCWGG 2 cut(s) 183, 393
BseCI ATCGAT 2 cut(s) 90, 519
BseDI CCNNGG 3 cut(s) 287, 325, 420
BseGI GGATG 3 cut(s) 368, 430, 743
BseJI GATNNNNATC 3 cut(s) 99, 591, 669
BseLI CCNNNNNNNGG 3 cut(s) 330, 356, 565
BseMII CTCAG 4 cut(s) 296, 626, 1059, 1131
BseNI ACTGG 5 cut(s) 323, 350, 660, 853, 1067
BseSI GKGCMC 1 cut(s) 227
BseX3I CGGCCG 1 cut(s) 888
BseXI GCAGC 4 cut(s) 85, 218, 457, 996
BseYI CCCAGC 1 cut(s) 616
Bsh1236I CGCG 1 cut(s) 381
Bsh1285I CGRYCG 1 cut(s) 891
BshFI GGCC 6 cut(s) 64, 305, 391, 829, 890, 951
BshTI ACCGGT 1 cut(s) 678
BshVI ATCGAT 2 cut(s) 90, 519
BsiEI CGRYCG 1 cut(s) 891
BsiHKCI CYCGRG 1 cut(s) 324
BsiSI CCGG 3 cut(s) 679, 891, 1100
BslFI GGGAC 2 cut(s) 346, 496
BslI CCNNNNNNNGG 3 cut(s) 330, 356, 565
BsmAI GTCTC 2 cut(s) 285, 1126
BsmFI GGGAC 2 cut(s) 346, 496
BsnI GGCC 6 cut(s) 64, 305, 391, 829, 890, 951
BsoBI CYCGRG 1 cut(s) 324
Bsp1286I GDGCHC 2 cut(s) 227, 1044
Bsp1720I GCTNAGC 1 cut(s) 612
Bsp19I CCATGG 1 cut(s) 420
BspACI CCGC 5 cut(s) 340, 533, 690, 798, 887
BspANI GGCC 6 cut(s) 64, 305, 391, 829, 890, 951
BspCNI CTCAG 4 cut(s) 295, 625, 1058, 1130
BspDI ATCGAT 2 cut(s) 90, 519
BspFNI CGCG 1 cut(s) 381
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 4 cut(s) 323, 334, 335, 1114
BspPI GGATC 2 cut(s) 1043, 1091
BsrBI CCGCTC 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 678
BsrI ACTGG 5 cut(s) 323, 350, 660, 853, 1067
BssAI RCCGGY 1 cut(s) 678
BssECI CCNNGG 3 cut(s) 287, 325, 420
BssNI GRCGYC 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 420
Bst2UI CCWGG 2 cut(s) 183, 393
Bst4CI ACNGT 3 cut(s) 14, 763, 902
Bst6I CTCTTC 4 cut(s) 49, 380, 454, 1129
BstACI GRCGYC 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 306
BstC8I GCNNGC 4 cut(s) 213, 307, 311, 777
BstDEI CTNAG 4 cut(s) 282, 612, 1045, 1117
BstDSI CCRYGG 1 cut(s) 420
BstF5I GGATG 3 cut(s) 368, 430, 743
BstFNI CGCG 1 cut(s) 381
BstH2I RGCGCY 1 cut(s) 316
BstHHI GCGC 2 cut(s) 315, 381
BstMAI GTCTC 2 cut(s) 285, 1126
BstMCI CGRYCG 1 cut(s) 891
BstMWI GCNNNNNNNGC 7 cut(s) 70, 208, 231, 306, 835, 914, 1039
BstNI CCWGG 2 cut(s) 183, 393
BstSCI CCNGG 2 cut(s) 181, 391
BstSFI CTRYAG 1 cut(s) 271
BstSLI GKGCMC 1 cut(s) 227
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 4 cut(s) 85, 218, 457, 996
BstV2I GAAGAC 2 cut(s) 132, 432
BstX2I RGATCY 2 cut(s) 874, 1048
BstYI RGATCY 2 cut(s) 874, 1048
BstZI CGGCCG 1 cut(s) 888
Bsu15I ATCGAT 2 cut(s) 90, 519
BsuI GTATCC 1 cut(s) 562
BsuRI GGCC 6 cut(s) 64, 305, 391, 829, 890, 951
BsuTUI ATCGAT 2 cut(s) 90, 519
BtgI CCRYGG 1 cut(s) 420
BtgZI GCGATG 1 cut(s) 687
BtrI CACGTC 1 cut(s) 928
BtsCI GGATG 3 cut(s) 368, 430, 743
BtsIMutI CAGTG 2 cut(s) 667, 1127
Cac8I GCNNGC 4 cut(s) 213, 307, 311, 777
CciI TCATGA 1 cut(s) 19
CfoI GCGC 2 cut(s) 315, 381
Cfr10I RCCGGY 1 cut(s) 678
Cfr13I GGNCC 4 cut(s) 321, 333, 827, 1113
ClaI ATCGAT 2 cut(s) 90, 519
CsiI ACCWGGT 1 cut(s) 181
Csp6I GTAC 2 cut(s) 645, 735
CspAI ACCGGT 1 cut(s) 678
CviAII CATG 5 cut(s) 20, 421, 727, 944, 1094
CviQI GTAC 2 cut(s) 645, 735
DdeI CTNAG 4 cut(s) 282, 612, 1045, 1117
DrdI GACNNNNNNGTC 1 cut(s) 154
DseDI GACNNNNNNGTC 1 cut(s) 154
EaeI YGGCCR 3 cut(s) 62, 888, 949
EagI CGGCCG 1 cut(s) 888
Eam1104I CTCTTC 4 cut(s) 49, 380, 454, 1129
EarI CTCTTC 4 cut(s) 49, 380, 454, 1129
EclXI CGGCCG 1 cut(s) 888
Eco130I CCWWGG 1 cut(s) 420
Eco147I AGGCCT 2 cut(s) 305, 391
Eco24I GRGCYC 1 cut(s) 1044
Eco47I GGWCC 3 cut(s) 321, 333, 1113
Eco47III AGCGCT 1 cut(s) 314
Eco52I CGGCCG 1 cut(s) 888
Eco57I CTGAAG 2 cut(s) 435, 561
Eco88I CYCGRG 1 cut(s) 324
EcoO109I RGGNCCY 3 cut(s) 333, 827, 1113
EcoRII CCWGG 2 cut(s) 181, 391
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 1044
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 5 cut(s) 23, 424, 730, 947, 1097
FaqI GGGAC 2 cut(s) 346, 496
FatI CATG 5 cut(s) 19, 420, 726, 943, 1093
FauI CCCGC 1 cut(s) 697
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 5 cut(s) 74, 232, 471, 888, 985
FokI GGATG 3 cut(s) 375, 437, 730
FriOI GRGCYC 1 cut(s) 1044
Fsp4HI GCNGC 5 cut(s) 74, 232, 471, 888, 985
FspBI CTAG 4 cut(s) 459, 552, 822, 1135
GlaI GCGC 2 cut(s) 314, 380
GluI GCNGC 5 cut(s) 74, 232, 471, 888, 985
GsaI CCCAGC 1 cut(s) 620
GsuI CTGGAG 1 cut(s) 182
HaeII RGCGCY 1 cut(s) 316
HaeIII GGCC 6 cut(s) 64, 305, 391, 829, 890, 951
HapII CCGG 3 cut(s) 679, 891, 1100
HhaI GCGC 2 cut(s) 315, 381
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 5 cut(s) 23, 424, 730, 947, 1097
Hin6I GCGC 2 cut(s) 313, 379
HinP1I GCGC 2 cut(s) 313, 379
HinfI GANTC 5 cut(s) 163, 236, 601, 1018, 1075
HpaII CCGG 3 cut(s) 679, 891, 1100
HphI GGTGA 1 cut(s) 914
Hpy188I TCNGA 3 cut(s) 168, 1023, 1048
Hpy188III TCNNGA 2 cut(s) 20, 188
Hpy99I CGWCG 4 cut(s) 151, 154, 157, 160
HpyAV CCTTC 1 cut(s) 720
HpyCH4III ACNGT 3 cut(s) 14, 763, 902
HpyCH4IV ACGT 3 cut(s) 149, 345, 927
HpyCH4V TGCA 3 cut(s) 73, 300, 309
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 208, 231, 306, 835, 914, 1039
HpyF3I CTNAG 4 cut(s) 282, 612, 1045, 1117
HpySE526I ACGT 3 cut(s) 149, 345, 927
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 5 cut(s) 23, 424, 730, 947, 1097
HspAI GCGC 2 cut(s) 313, 379
KflI GGGWCCC 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 16
LmnI GCTCC 3 cut(s) 207, 760, 1030
Lsp1109I GCAGC 4 cut(s) 85, 218, 457, 996
LweI GCATC 2 cut(s) 60, 353
MabI ACCWGGT 1 cut(s) 181
MaeI CTAG 4 cut(s) 459, 552, 822, 1135
MaeII ACGT 3 cut(s) 149, 345, 927
MaeIII GTNAC 2 cut(s) 257, 554
MbiI CCGCTC 1 cut(s) 535
MflI RGATCY 2 cut(s) 874, 1048
MhlI GDGCHC 2 cut(s) 227, 1044
MlsI TGGCCA 2 cut(s) 64, 951
MluCI AATT 8 cut(s) 487, 499, 667, 697, 715, 785, 1009, 1087
MluNI TGGCCA 2 cut(s) 64, 951
MlyI GAGTC 1 cut(s) 1069
MmeI TCCRAC 2 cut(s) 229, 782
Mox20I TGGCCA 2 cut(s) 64, 951
MroXI GAANNNNTTC 1 cut(s) 876
MscI TGGCCA 2 cut(s) 64, 951
MseI TTAA 2 cut(s) 477, 684
Msp20I TGGCCA 2 cut(s) 64, 951
MspA1I CMGCKG 1 cut(s) 616
MspI CCGG 3 cut(s) 679, 891, 1100
MspR9I CCNGG 2 cut(s) 183, 393
MvaI CCWGG 2 cut(s) 183, 393
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 7 cut(s) 70, 208, 231, 306, 835, 914, 1039
NcoI CCATGG 1 cut(s) 420
NlaIII CATG 5 cut(s) 23, 424, 730, 947, 1097
NlaIV GGNNCC 4 cut(s) 323, 334, 335, 1114
NmeAIII GCCGAG 1 cut(s) 312
PagI TCATGA 1 cut(s) 19
PceI AGGCCT 2 cut(s) 305, 391
PcsI WCGNNNNNNNCGW 2 cut(s) 152, 158
PdmI GAANNNNTTC 1 cut(s) 876
PfeI GAWTC 4 cut(s) 163, 236, 601, 1018
PflFI GACNNNGTC 1 cut(s) 151
PflMI CCANNNNNTGG 1 cut(s) 356
PinAI ACCGGT 1 cut(s) 678
PkrI GCNGC 5 cut(s) 75, 233, 472, 889, 986
PleI GAGTC 1 cut(s) 1069
PpsI GAGTC 1 cut(s) 1069
PpuMI RGGWCCY 2 cut(s) 333, 1113
Psp1406I AACGTT 1 cut(s) 345
Psp5II RGGWCCY 2 cut(s) 333, 1113
Psp6I CCWGG 2 cut(s) 181, 391
PspFI CCCAGC 1 cut(s) 616
PspGI CCWGG 2 cut(s) 181, 391
PspN4I GGNNCC 4 cut(s) 323, 334, 335, 1114
PspPI GGNCC 4 cut(s) 321, 333, 827, 1113
PspPPI RGGWCCY 2 cut(s) 333, 1113
PsuI RGATCY 2 cut(s) 874, 1048
PsyI GACNNNGTC 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 616
RsaI GTAC 2 cut(s) 646, 736
RsaNI GTAC 2 cut(s) 645, 735
SaqAI TTAA 2 cut(s) 477, 684
SatI GCNGC 5 cut(s) 74, 232, 471, 888, 985
Sau96I GGNCC 4 cut(s) 321, 333, 827, 1113
ScaI AGTACT 1 cut(s) 736
SchI GAGTC 1 cut(s) 1069
ScrFI CCNGG 2 cut(s) 183, 393
SduI GDGCHC 2 cut(s) 227, 1044
SexAI ACCWGGT 1 cut(s) 181
SfaNI GCATC 2 cut(s) 60, 353
SfcI CTRYAG 1 cut(s) 271
SinI GGWCC 3 cut(s) 321, 333, 1113
Sse9I AATT 8 cut(s) 487, 499, 667, 697, 715, 785, 1009, 1087
SseBI AGGCCT 2 cut(s) 305, 391
SsiI CCGC 5 cut(s) 340, 533, 690, 798, 887
SspMI CTAG 4 cut(s) 459, 552, 822, 1135
StuI AGGCCT 2 cut(s) 305, 391
StyD4I CCNGG 2 cut(s) 181, 391
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 3 cut(s) 14, 763, 902
TaiI ACGT 3 cut(s) 152, 348, 930
TaqI TCGA 7 cut(s) 90, 146, 398, 519, 673, 920, 1073
TasI AATT 8 cut(s) 487, 499, 667, 697, 715, 785, 1009, 1087
TatI WGTACW 1 cut(s) 734
TauI GCSGC 1 cut(s) 890
TfiI GAWTC 4 cut(s) 163, 236, 601, 1018
Tru1I TTAA 2 cut(s) 477, 684
Tru9I TTAA 2 cut(s) 477, 684
TscAI CASTG 2 cut(s) 667, 1134
TseI GCWGC 4 cut(s) 73, 231, 470, 984
TspDTI ATGAA 6 cut(s) 132, 581, 621, 690, 778, 1022
TspGWI ACGGA 2 cut(s) 426, 1043
TspRI CASTG 2 cut(s) 667, 1134
Tth111I GACNNNGTC 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 356
VpaK11BI GGWCC 3 cut(s) 321, 333, 1113
XapI RAATTY 2 cut(s) 697, 785
XmnI GAANNNNTTC 1 cut(s) 876
XspI CTAG 4 cut(s) 459, 552, 822, 1135
ZraI GACGTC 1 cut(s) 150
ZrmI AGTACT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.