MD10G1341000.v1.1

Domain of unknown function (DUF2431)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016278
Physical Location & Seq
Forward (+)
41615475 .. 41616770
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1341000.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGGAAAAAAGGAAGAAGATTACAAAGAACAGAGGGAAGCAGACGCAGAAACAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAATCGGATGAAGAAGAGAAATGGAGAAAGCACTATTCGTCTGGGCAGAGGATATTGTTGGTGGGCGAAGGAGACTTCTCCTTCTCTGTTTCTTTGGCCACTCGCTTTGGATCTGCCCGCAACATGCTTGCCACTTCTCTCGATTCCCAGGAGAAGATCGCTGACAAGTACAGTAACGCCATGGAGAATGTGAGGAAGCTTGAAGAAAAGGGATGCATCGTGATTTACGGAGTGGACGCCCAGCTTATGAGCCAACACTTCTTCCTCGCCACTCAGAGGTTTGACCGCATCGTCTACAACTTCCCTCATGTTGGCTTTCTCTTCCGCGAGGATAGCTTCTGTCAGATCCAGTTGAACAAGAGATTGGTGAAGGGTTTCTTCAAAAATGCCAAATTACTCCTGAGAAAAGAGGGTGGAGGAGAGATTCACATAACCCACAAAGAGGGTCATCCTTACAACAAATGGGATTTGATCAGGAAGGCACACAAGATTGGCCTGCAAATCGATCGATCTGTGCCCTTCTGCAAGGACGACTATCCCGGCTACCACAACAAGAGAGGCCACGGCATCCATTCCGACGCCCCCTTCCACCTTGGCGATTGCACTACTTACATGTTCAAAATCAGGCTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

239

Amino Acids

27.75

Weight (kDa)

6.02

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 46 - 213 1.6e-55 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016278)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00200
malus_domestica MD10G1341000.v1.1
prunus_persica Prupe.4G002200_v2.0.a1
pyrus_communis pycom10g29060
rosa_chinensis RchiOBHm_Chr5g0000261
rosa_laevigata RLG00000030854
rosa_multiflora Rmu_sc0043092.1_g000001 Rmu_ssc0000385.1_g000003
rosa_roxburghii Rroxscaffold_1G00075840
rosa_rugosa Rorug04G0382900
rosa_samantha Rh5AG001900 Rh5BG003300 Rh5CG002200 Rh5DG002200
rosa_wichuraiana Rw5G000240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 371
AccI GTMKAC 1 cut(s) 375
AccII CGCG 1 cut(s) 408
AciI CCGC 3 cut(s) 199, 367, 406
AclWI GGATC 2 cut(s) 199, 421
AcoI YGGCCR 1 cut(s) 177
AcyI GRCGYC 2 cut(s) 318, 660
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 3 cut(s) 357, 392, 523
AflIII ACRYGT 1 cut(s) 693
AgsI TTSAA 4 cut(s) 284, 436, 463, 700
AjnI CCWGG 1 cut(s) 228
AjuI GAANNNNNNNTTGG 2 cut(s) 428, 460
AluBI AGCT 3 cut(s) 280, 325, 417
AluI AGCT 3 cut(s) 280, 325, 417
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 2 cut(s) 199, 421
AoxI GGCC 3 cut(s) 177, 574, 640
ApeKI GCWGC 1 cut(s) 709
Asp700I GAANNNNTTC 1 cut(s) 455
AsuC2I CCSGG 1 cut(s) 621
AsuHPI GGTGA 1 cut(s) 460
BaeGI GKGCMC 1 cut(s) 600
BalI TGGCCA 1 cut(s) 179
BbvI GCAGC 1 cut(s) 696
BceAI ACGGC 1 cut(s) 661
BcgI CGANNNNNNTGC 2 cut(s) 569, 603
BciT130I CCWGG 1 cut(s) 230
BclI TGATCA 1 cut(s) 552
BcnI CCSGG 1 cut(s) 621
BcoDI GTCTC 1 cut(s) 147
BisI GCNGC 1 cut(s) 710
BlsI GCNGC 1 cut(s) 711
Bme1390I CCNGG 2 cut(s) 230, 621
BmrFI CCNGG 2 cut(s) 230, 621
BmsI GCATC 4 cut(s) 284, 306, 378, 657
BpuMI CCSGG 1 cut(s) 621
Bsa29I ATCGAT 2 cut(s) 585, 589
BsaHI GRCGYC 2 cut(s) 318, 660
BsaJI CCNNGG 4 cut(s) 228, 261, 643, 673
Bsc4I CCNNNNNNNGG 3 cut(s) 357, 392, 523
Bse1I ACTGG 1 cut(s) 430
BseBI CCWGG 1 cut(s) 230
BseCI ATCGAT 2 cut(s) 585, 589
BseDI CCNNGG 4 cut(s) 228, 261, 643, 673
BseGI GGATG 4 cut(s) 94, 299, 529, 648
BseLI CCNNNNNNNGG 3 cut(s) 357, 392, 523
BseMII CTCAG 2 cut(s) 368, 473
BseNI ACTGG 1 cut(s) 430
BseRI GAGGAG 1 cut(s) 513
BseSI GKGCMC 1 cut(s) 600
BseXI GCAGC 1 cut(s) 696
BseYI CCCAGC 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 408
Bsh1285I CGRYCG 1 cut(s) 589
BshFI GGCC 3 cut(s) 179, 576, 642
BshVI ATCGAT 2 cut(s) 585, 589
BsiEI CGRYCG 1 cut(s) 589
BsiSI CCGG 1 cut(s) 621
BslI CCNNNNNNNGG 3 cut(s) 357, 392, 523
BsmAI GTCTC 1 cut(s) 147
BsnI GGCC 3 cut(s) 179, 576, 642
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 6 cut(s) 191, 237, 426, 552, 586, 590
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 3 cut(s) 199, 367, 406
BspANI GGCC 3 cut(s) 179, 576, 642
BspCNI CTCAG 2 cut(s) 367, 474
BspDI ATCGAT 2 cut(s) 585, 589
BspFNI CGCG 1 cut(s) 408
BspPI GGATC 2 cut(s) 199, 421
BsrI ACTGG 1 cut(s) 430
BssECI CCNNGG 4 cut(s) 228, 261, 643, 673
BssMI GATC 6 cut(s) 191, 237, 426, 552, 586, 590
BssNI GRCGYC 2 cut(s) 318, 660
BssT1I CCWWGG 2 cut(s) 261, 673
Bst2UI CCWGG 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 254
Bst6I CTCTTC 2 cut(s) 90, 407
BstACI GRCGYC 2 cut(s) 318, 660
BstC8I GCNNGC 3 cut(s) 199, 210, 578
BstDEI CTNAG 2 cut(s) 354, 482
BstDSI CCRYGG 2 cut(s) 261, 643
BstF5I GGATG 4 cut(s) 94, 299, 529, 648
BstFNI CGCG 1 cut(s) 408
BstKTI GATC 6 cut(s) 194, 240, 429, 555, 589, 593
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 6 cut(s) 191, 237, 426, 552, 586, 590
BstMCI CGRYCG 1 cut(s) 589
BstMWI GCNNNNNNNGC 1 cut(s) 414
BstNI CCWGG 1 cut(s) 230
BstNSI RCATGY 2 cut(s) 208, 697
BstSCI CCNGG 2 cut(s) 228, 619
BstSLI GKGCMC 1 cut(s) 600
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 696
BstX2I RGATCY 2 cut(s) 191, 426
BstYI RGATCY 2 cut(s) 191, 426
Bsu15I ATCGAT 2 cut(s) 585, 589
BsuRI GGCC 3 cut(s) 179, 576, 642
BsuTUI ATCGAT 2 cut(s) 585, 589
BtgI CCRYGG 2 cut(s) 261, 643
BtsCI GGATG 4 cut(s) 94, 299, 529, 648
Cac8I GCNNGC 3 cut(s) 199, 210, 578
ClaI ATCGAT 2 cut(s) 585, 589
CseI GACGC 3 cut(s) 53, 326, 668
Csp6I GTAC 1 cut(s) 250
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 4 cut(s) 205, 262, 389, 694
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 2 cut(s) 354, 482
DpnI GATC 6 cut(s) 193, 239, 428, 554, 588, 592
DpnII GATC 6 cut(s) 191, 237, 426, 552, 586, 590
DrdI GACNNNNNNGTC 1 cut(s) 371
DseDI GACNNNNNNGTC 1 cut(s) 371
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 2 cut(s) 90, 407
EarI CTCTTC 2 cut(s) 90, 407
Eco130I CCWWGG 2 cut(s) 261, 673
EcoRII CCWGG 1 cut(s) 228
EcoT14I CCWWGG 2 cut(s) 261, 673
EcoT22I ATGCAT 1 cut(s) 299
ErhI CCWWGG 2 cut(s) 261, 673
FaeI CATG 4 cut(s) 208, 265, 392, 697
FaiI YATR 6 cut(s) 206, 263, 329, 390, 512, 695
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FatI CATG 4 cut(s) 204, 261, 388, 693
FauI CCCGC 1 cut(s) 206
FbaI TGATCA 1 cut(s) 552
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 1 cut(s) 710
FokI GGATG 4 cut(s) 101, 306, 516, 635
Fsp4HI GCNGC 1 cut(s) 710
GluI GCNGC 1 cut(s) 710
GsaI CCCAGC 1 cut(s) 325
HaeIII GGCC 3 cut(s) 179, 576, 642
HapII CCGG 1 cut(s) 621
HgaI GACGC 3 cut(s) 53, 326, 668
Hin1I GRCGYC 2 cut(s) 318, 660
Hin1II CATG 4 cut(s) 208, 265, 392, 697
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 3 cut(s) 83, 224, 505
HpaII CCGG 1 cut(s) 621
HphI GGTGA 1 cut(s) 460
Hpy166II GTNNAC 2 cut(s) 316, 376
Hpy188I TCNGA 4 cut(s) 88, 357, 426, 658
Hpy188III TCNNGA 4 cut(s) 221, 301, 481, 556
Hpy8I GTNNAC 2 cut(s) 316, 376
Hpy99I CGWCG 1 cut(s) 662
HpyAV CCTTC 6 cut(s) 143, 172, 445, 553, 610, 676
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4V TGCA 4 cut(s) 297, 580, 606, 684
HpyF10VI GCNNNNNNNGC 1 cut(s) 414
HpyF3I CTNAG 2 cut(s) 354, 482
Hsp92I GRCGYC 2 cut(s) 318, 660
Hsp92II CATG 4 cut(s) 208, 265, 392, 697
Ksp22I TGATCA 1 cut(s) 552
Kzo9I GATC 6 cut(s) 191, 237, 426, 552, 586, 590
Lsp1109I GCAGC 1 cut(s) 696
LweI GCATC 4 cut(s) 284, 306, 378, 657
MaeIII GTNAC 1 cut(s) 254
MalI GATC 6 cut(s) 193, 239, 428, 554, 588, 592
MboI GATC 6 cut(s) 191, 237, 426, 552, 586, 590
MflI RGATCY 2 cut(s) 191, 426
MhlI GDGCHC 1 cut(s) 600
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 1 cut(s) 473
MluNI TGGCCA 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 681
Mox20I TGGCCA 1 cut(s) 179
Mph1103I ATGCAT 1 cut(s) 299
MroXI GAANNNNTTC 1 cut(s) 455
MscI TGGCCA 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 179
MspI CCGG 1 cut(s) 621
MspR9I CCNGG 2 cut(s) 230, 621
MvaI CCWGG 1 cut(s) 230
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 1 cut(s) 414
NciI CCSGG 1 cut(s) 621
NcoI CCATGG 1 cut(s) 261
NdeII GATC 6 cut(s) 191, 237, 426, 552, 586, 590
NlaIII CATG 4 cut(s) 208, 265, 392, 697
NsiI ATGCAT 1 cut(s) 299
NspI RCATGY 2 cut(s) 208, 697
PciI ACATGT 1 cut(s) 693
PdmI GAANNNNTTC 1 cut(s) 455
PfeI GAWTC 3 cut(s) 83, 224, 505
PkrI GCNGC 1 cut(s) 711
Ple19I CGATCG 1 cut(s) 589
PscI ACATGT 1 cut(s) 693
Psp6I CCWGG 1 cut(s) 228
PspFI CCCAGC 1 cut(s) 321
PspGI CCWGG 1 cut(s) 228
PsuI RGATCY 2 cut(s) 191, 426
PvuI CGATCG 1 cut(s) 589
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SatI GCNGC 1 cut(s) 710
Sau3AI GATC 6 cut(s) 191, 237, 426, 552, 586, 590
ScrFI CCNGG 2 cut(s) 230, 621
SduI GDGCHC 1 cut(s) 600
SetI ASST 5 cut(s) 282, 327, 362, 419, 675
SfaNI GCATC 4 cut(s) 284, 306, 378, 657
Sse9I AATT 1 cut(s) 473
SsiI CCGC 3 cut(s) 199, 367, 406
StyD4I CCNGG 2 cut(s) 228, 619
StyI CCWWGG 2 cut(s) 261, 673
TaaI ACNGT 1 cut(s) 254
TaqI TCGA 3 cut(s) 222, 585, 589
TasI AATT 1 cut(s) 473
TatI WGTACW 1 cut(s) 249
TfiI GAWTC 3 cut(s) 83, 224, 505
TseI GCWGC 1 cut(s) 709
TspDTI ATGAA 1 cut(s) 105
TspGWI ACGGA 1 cut(s) 324
XceI RCATGY 2 cut(s) 208, 697
XmiI GTMKAC 1 cut(s) 375
XmnI GAANNNNTTC 1 cut(s) 455
Zsp2I ATGCAT 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.