RchiOBHm_Chr5g0000261

Domain of unknown function (DUF2431)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016278
Physical Location & Seq
Reverse (-)
172820 .. 174487
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28183

Sequence Viewer

Length: 738 bp
ATGGGAAGAGAAGACGAAGAAGAAGAAGAAGAAGAAGAAGAAGAATACCAACAAGAAAGGGAAACTGATGATGATGATGATGAATCTTCAGCTTCAGAGGAAGAGGAAGAATCAGAATCGGAAGAGAAATGGAGAAAGCACTACTCATCCAGGCATAGGATATTGTTGGTCGGAGAAGGAGACTTCTCCTTCTCTGTGTCTTTAGCCACACACTTCGGATCAGCGCGCAACATGGTCGCCACCTCTCTCGATTCCCAGGAGAAGATATGTAGCAAGTACAGTAACGCAATAGCGAATGTGAGGGAGCTTGAAGAAAGGGGATGCTTAGTGCTCTACGGAGTTGACGCCAAGACCATGAGTCAACACTACTTCCTCAGCACTCAGAGGTTCGACAGAATTGTCTACAACTTCCCTCATGTCGGCTTTCTCTTCCGGGAGGATAGCTACTGCCAGATCCAGTTGAACAAGAGATTGGTCAAGGGTTTTCTCAGGAATGCTAAACTTCTTTTGAAAAAAGAGGGAGGGCAGATTCATATCTCTCACAAAGAAGGCCATCCTTACAACAAATGGGATTTAGTGAAGAAAGCACACAAGATTGGAGGACTCGTTCTGACCCAATCTGTGCCCTTCTGCAAGGATGACTATCCTGGATATGACAACAAGAGAGCCCATGGTACCCTTTCCGATGCCTCTTTCCACCTTGGGGATTGCACTACTTACATGTTTAGCCTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

245

Amino Acids

28.3

Weight (kDa)

5.14

Isoelectric Point (pI)

64.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 55 - 222 4.1e-54 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016278)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00200
malus_domestica MD10G1341000.v1.1
prunus_persica Prupe.4G002200_v2.0.a1
pyrus_communis pycom10g29060
rosa_chinensis RchiOBHm_Chr5g0000261
rosa_laevigata RLG00000030854
rosa_multiflora Rmu_sc0043092.1_g000001 Rmu_ssc0000385.1_g000003
rosa_roxburghii Rroxscaffold_1G00075840
rosa_rugosa Rorug04G0382900
rosa_samantha Rh5AG001900 Rh5BG003300 Rh5CG002200 Rh5DG002200
rosa_wichuraiana Rw5G000240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
Acc65I GGTACC 1 cut(s) 674
AccB1I GGYRCC 1 cut(s) 674
AccI GTMKAC 1 cut(s) 402
AccII CGCG 1 cut(s) 226
AclWI GGATC 2 cut(s) 226, 448
AcuI CTGAAG 2 cut(s) 72, 78
AcyI GRCGYC 1 cut(s) 345
AfaI GTAC 2 cut(s) 278, 676
AfiI CCNNNNNNNGG 3 cut(s) 156, 419, 703
AflIII ACRYGT 1 cut(s) 720
AgsI TTSAA 3 cut(s) 311, 463, 511
AhdI GACNNNNNGTC 1 cut(s) 357
AjnI CCWGG 3 cut(s) 149, 255, 646
AjuI GAANNNNNNNTTGG 2 cut(s) 455, 487
AloI GAACNNNNNNTCC 2 cut(s) 591, 623
AluBI AGCT 3 cut(s) 92, 307, 444
AluI AGCT 3 cut(s) 92, 307, 444
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 2 cut(s) 226, 448
AoxI GGCC 1 cut(s) 550
Asp718I GGTACC 1 cut(s) 674
AspLEI GCGC 2 cut(s) 226, 228
AsuC2I CCSGG 1 cut(s) 434
BaeGI GKGCMC 1 cut(s) 627
BanI GGYRCC 1 cut(s) 674
BanII GRGCYC 1 cut(s) 670
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 333
BbvCI CCTCAGC 1 cut(s) 374
BccI CCATC 1 cut(s) 561
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 3 cut(s) 151, 257, 648
BcnI CCSGG 1 cut(s) 434
BcoDI GTCTC 1 cut(s) 174
Bme1390I CCNGG 4 cut(s) 151, 257, 434, 648
BmeRI GACNNNNNGTC 1 cut(s) 357
BmiI GGNNCC 1 cut(s) 676
BmrFI CCNGG 4 cut(s) 151, 257, 434, 648
BmsI GCATC 2 cut(s) 311, 676
BpiI GAAGAC 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 434
BsaBI GATNNNNATC 2 cut(s) 533, 642
BsaHI GRCGYC 1 cut(s) 345
BsaJI CCNNGG 3 cut(s) 255, 670, 700
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 419, 703
Bse1I ACTGG 1 cut(s) 457
Bse8I GATNNNNATC 2 cut(s) 533, 642
BseBI CCWGG 3 cut(s) 151, 257, 648
BseDI CCNNGG 3 cut(s) 255, 670, 700
BseGI GGATG 4 cut(s) 146, 326, 553, 643
BseJI GATNNNNATC 2 cut(s) 533, 642
BseLI CCNNNNNNNGG 3 cut(s) 156, 419, 703
BseMII CTCAG 3 cut(s) 388, 395, 502
BseNI ACTGG 1 cut(s) 457
BsePI GCGCGC 1 cut(s) 224
BseSI GKGCMC 1 cut(s) 627
Bsh1236I CGCG 1 cut(s) 226
BshFI GGCC 1 cut(s) 552
BshNI GGYRCC 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 1 cut(s) 433
BslI CCNNNNNNNGG 3 cut(s) 156, 419, 703
BsmAI GTCTC 1 cut(s) 174
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 1 cut(s) 552
Bsp1286I GDGCHC 3 cut(s) 333, 627, 670
Bsp143I GATC 2 cut(s) 218, 453
Bsp19I CCATGG 1 cut(s) 670
BspANI GGCC 1 cut(s) 552
BspCNI CTCAG 3 cut(s) 387, 394, 501
BspFNI CGCG 1 cut(s) 226
BspLI GGNNCC 1 cut(s) 676
BspPI GGATC 2 cut(s) 226, 448
BspT107I GGYRCC 1 cut(s) 674
BsrI ACTGG 1 cut(s) 457
BssECI CCNNGG 3 cut(s) 255, 670, 700
BssHII GCGCGC 1 cut(s) 224
BssMI GATC 2 cut(s) 218, 453
BssNI GRCGYC 1 cut(s) 345
BssT1I CCWWGG 2 cut(s) 670, 700
Bst2UI CCWGG 3 cut(s) 151, 257, 648
Bst4CI ACNGT 1 cut(s) 281
Bst6I CTCTTC 3 cut(s) 96, 117, 434
BstACI GRCGYC 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 5 cut(s) 325, 374, 381, 488, 735
BstDSI CCRYGG 1 cut(s) 670
BstF5I GGATG 4 cut(s) 146, 326, 553, 643
BstFNI CGCG 1 cut(s) 226
BstHHI GCGC 2 cut(s) 226, 228
BstKTI GATC 2 cut(s) 221, 456
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 2 cut(s) 218, 453
BstNI CCWGG 3 cut(s) 151, 257, 648
BstNSI RCATGY 1 cut(s) 724
BstSCI CCNGG 4 cut(s) 149, 255, 432, 646
BstSLI GKGCMC 1 cut(s) 627
BstUI CGCG 1 cut(s) 226
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 453
BstYI RGATCY 1 cut(s) 453
BsuRI GGCC 1 cut(s) 552
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 4 cut(s) 146, 326, 553, 643
Cac8I GCNNGC 1 cut(s) 226
CfoI GCGC 2 cut(s) 226, 228
CseI GACGC 1 cut(s) 353
Csp6I GTAC 2 cut(s) 277, 675
CviAII CATG 5 cut(s) 232, 355, 416, 671, 721
CviJI RGCY 8 cut(s) 92, 206, 307, 423, 444, 552, 668, 729
CviKI_1 RGCY 8 cut(s) 92, 206, 307, 423, 444, 552, 668, 729
CviQI GTAC 2 cut(s) 277, 675
DdeI CTNAG 5 cut(s) 325, 374, 381, 488, 735
DpnI GATC 2 cut(s) 220, 455
DpnII GATC 2 cut(s) 218, 453
DrdI GACNNNNNNGTC 1 cut(s) 398
DriI GACNNNNNGTC 1 cut(s) 357
DseDI GACNNNNNNGTC 1 cut(s) 398
Eam1104I CTCTTC 3 cut(s) 96, 117, 434
Eam1105I GACNNNNNGTC 1 cut(s) 357
EarI CTCTTC 3 cut(s) 96, 117, 434
Eco130I CCWWGG 2 cut(s) 670, 700
Eco24I GRGCYC 1 cut(s) 670
Eco57I CTGAAG 2 cut(s) 72, 78
EcoRII CCWGG 3 cut(s) 149, 255, 646
EcoT14I CCWWGG 2 cut(s) 670, 700
EcoT38I GRGCYC 1 cut(s) 670
ErhI CCWWGG 2 cut(s) 670, 700
FaeI CATG 5 cut(s) 235, 358, 419, 674, 724
FaiI YATR 9 cut(s) 156, 233, 268, 356, 417, 534, 654, 672, 722
FatI CATG 5 cut(s) 231, 354, 415, 670, 720
FblI GTMKAC 1 cut(s) 402
FokI GGATG 4 cut(s) 133, 333, 540, 650
FriOI GRGCYC 1 cut(s) 670
GlaI GCGC 2 cut(s) 225, 227
HaeIII GGCC 1 cut(s) 552
HapII CCGG 1 cut(s) 433
HgaI GACGC 1 cut(s) 353
HhaI GCGC 2 cut(s) 226, 228
Hin1I GRCGYC 1 cut(s) 345
Hin1II CATG 5 cut(s) 235, 358, 419, 674, 724
Hin6I GCGC 2 cut(s) 224, 226
HinP1I GCGC 2 cut(s) 224, 226
HincII GTYRAC 2 cut(s) 343, 362
HindII GTYRAC 2 cut(s) 343, 362
HinfI GANTC 7 cut(s) 83, 110, 116, 251, 358, 529, 603
HpaII CCGG 1 cut(s) 433
Hpy166II GTNNAC 3 cut(s) 343, 362, 403
Hpy188I TCNGA 8 cut(s) 97, 115, 121, 173, 218, 384, 612, 685
Hpy188III TCNNGA 2 cut(s) 248, 490
Hpy8I GTNNAC 3 cut(s) 343, 362, 403
HpyAV CCTTC 4 cut(s) 170, 199, 542, 637
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4V TGCA 2 cut(s) 633, 711
HpyF3I CTNAG 5 cut(s) 325, 374, 381, 488, 735
Hsp92I GRCGYC 1 cut(s) 345
Hsp92II CATG 5 cut(s) 235, 358, 419, 674, 724
HspAI GCGC 2 cut(s) 224, 226
KpnI GGTACC 1 cut(s) 678
Kzo9I GATC 2 cut(s) 218, 453
LmnI GCTCC 1 cut(s) 304
LweI GCATC 2 cut(s) 311, 676
MaeIII GTNAC 1 cut(s) 281
MalI GATC 2 cut(s) 220, 455
MboI GATC 2 cut(s) 218, 453
MflI RGATCY 1 cut(s) 453
MhlI GDGCHC 3 cut(s) 333, 627, 670
MluCI AATT 1 cut(s) 396
MlyI GAGTC 2 cut(s) 367, 597
MmeI TCCRAC 1 cut(s) 151
MspI CCGG 1 cut(s) 433
MspR9I CCNGG 4 cut(s) 151, 257, 434, 648
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 3 cut(s) 151, 257, 648
MvnI CGCG 1 cut(s) 226
NciI CCSGG 1 cut(s) 434
NcoI CCATGG 1 cut(s) 670
NdeII GATC 2 cut(s) 218, 453
NlaIII CATG 5 cut(s) 235, 358, 419, 674, 724
NlaIV GGNNCC 1 cut(s) 676
NspI RCATGY 1 cut(s) 724
PauI GCGCGC 1 cut(s) 224
PciI ACATGT 1 cut(s) 720
PctI GAATGC 1 cut(s) 499
PfeI GAWTC 5 cut(s) 83, 110, 116, 251, 529
PfoI TCCNGGA 2 cut(s) 432, 646
PleI GAGTC 2 cut(s) 366, 597
PpsI GAGTC 2 cut(s) 366, 597
PscI ACATGT 1 cut(s) 720
Psp6I CCWGG 3 cut(s) 149, 255, 646
PspGI CCWGG 3 cut(s) 149, 255, 646
PspN4I GGNNCC 1 cut(s) 676
PsuI RGATCY 1 cut(s) 453
PteI GCGCGC 1 cut(s) 224
RsaI GTAC 2 cut(s) 278, 676
RsaNI GTAC 2 cut(s) 277, 675
Sau3AI GATC 2 cut(s) 218, 453
SchI GAGTC 2 cut(s) 367, 597
ScrFI CCNGG 4 cut(s) 151, 257, 434, 648
SduI GDGCHC 3 cut(s) 333, 627, 670
SetI ASST 6 cut(s) 94, 245, 309, 389, 446, 702
SfaNI GCATC 2 cut(s) 311, 676
Sse9I AATT 1 cut(s) 396
StyD4I CCNGG 4 cut(s) 149, 255, 432, 646
StyI CCWWGG 2 cut(s) 670, 700
TaaI ACNGT 1 cut(s) 281
TaqI TCGA 2 cut(s) 249, 390
TasI AATT 1 cut(s) 396
TatI WGTACW 1 cut(s) 276
TfiI GAWTC 5 cut(s) 83, 110, 116, 251, 529
TspDTI ATGAA 2 cut(s) 96, 521
TspGWI ACGGA 1 cut(s) 351
XceI RCATGY 1 cut(s) 724
XmiI GTMKAC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.