Rroxscaffold_1G00075840

Domain of unknown function (DUF2431)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016278
Physical Location & Seq
Forward (+)
96426640 .. 96431272
4633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075840.1

Sequence Viewer

Length: 729 bp
ATGGGAAGAGAAGACGAAGAAGAAGAAGAAGAATACCAACAAGAAAGGGAAACTGATGATGATGATGATGATGATGATGAATCTTCAGCTTCAGAGGAAGAATCAGAATCGGAAGAGAAATGGAGAAAGCACTACTCATCCAGGCATAGGATATTGTTGGTCGGAGAAGGAGACTTCTCCTTCTCTCTGTCTTTAGCCACACACTTCGGATCAGCGCGCAACATGGTCGCCACCTCTCTCGATTCCCAGGAGAAGATATGTAGCAAGTACAGTAACGCAATAGTGAATGTGAGGGAGCTTGAAGCAAGGGGATGCATAGTGCTCTACGGAGTTGACGCCAAGACCATGAGTCAACACTACTTCCTCAGCACTCAGAGGTTCGACAGAATTGTCTACAACTTCCCTCATGTCGGCTTTCTCTTCCGGGAGGATAGCTACTGCCAGATCCAGTTGAACAAGAGATTGGTCAAGGGTTTTCTCCGGAATGCTAAACTTCTTTTGAAAAAAGAGGGAGGGCAGATTCATATCTCTCACAAAGAAGGCCATCCTTACAACAAATGGGATTTAGTGAAGAAAGCACACAAGATTGGAGGACTCGTTCTGACCCAATCTGCGCCCTTCTGCAAGGATGACTATCCTGGATATGACAACAAGAGAGCCCATGGTACCCTTTCCGATGCCTCTTTCCACCTTGGGGATTGCACTACTTACATGTTTAGCCTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

242

Amino Acids

27.83

Weight (kDa)

5.39

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 52 - 219 2e-53 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016278)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00200
malus_domestica MD10G1341000.v1.1
prunus_persica Prupe.4G002200_v2.0.a1
pyrus_communis pycom10g29060
rosa_chinensis RchiOBHm_Chr5g0000261
rosa_laevigata RLG00000030854
rosa_multiflora Rmu_sc0043092.1_g000001 Rmu_ssc0000385.1_g000003
rosa_roxburghii Rroxscaffold_1G00075840
rosa_rugosa Rorug04G0382900
rosa_samantha Rh5AG001900 Rh5BG003300 Rh5CG002200 Rh5DG002200
rosa_wichuraiana Rw5G000240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
Acc65I GGTACC 1 cut(s) 665
AccB1I GGYRCC 1 cut(s) 665
AccI GTMKAC 1 cut(s) 393
AccII CGCG 1 cut(s) 217
AccIII TCCGGA 1 cut(s) 480
AclWI GGATC 2 cut(s) 217, 439
AcuI CTGAAG 2 cut(s) 69, 75
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 2 cut(s) 269, 667
AfiI CCNNNNNNNGG 3 cut(s) 147, 410, 694
AflIII ACRYGT 1 cut(s) 711
AgsI TTSAA 3 cut(s) 302, 454, 502
AhdI GACNNNNNGTC 1 cut(s) 348
AjnI CCWGG 3 cut(s) 140, 246, 637
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
AluBI AGCT 3 cut(s) 89, 298, 435
AluI AGCT 3 cut(s) 89, 298, 435
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 2 cut(s) 217, 439
Aor13HI TCCGGA 1 cut(s) 480
AoxI GGCC 1 cut(s) 541
Asp718I GGTACC 1 cut(s) 665
AspLEI GCGC 3 cut(s) 217, 219, 616
AsuC2I CCSGG 1 cut(s) 425
BanI GGYRCC 1 cut(s) 665
BanII GRGCYC 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 324
BbvCI CCTCAGC 1 cut(s) 365
BccI CCATC 1 cut(s) 552
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 3 cut(s) 142, 248, 639
BcnI CCSGG 1 cut(s) 425
BcoDI GTCTC 1 cut(s) 165
Bme1390I CCNGG 4 cut(s) 142, 248, 425, 639
BmeRI GACNNNNNGTC 1 cut(s) 348
BmiI GGNNCC 1 cut(s) 667
BmrFI CCNGG 4 cut(s) 142, 248, 425, 639
BmsI GCATC 2 cut(s) 302, 667
BpiI GAAGAC 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 425
BsaBI GATNNNNATC 2 cut(s) 524, 633
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 3 cut(s) 246, 661, 691
BsaWI WCCGGW 1 cut(s) 480
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 3 cut(s) 147, 410, 694
Bse1I ACTGG 1 cut(s) 448
Bse8I GATNNNNATC 2 cut(s) 524, 633
BseAI TCCGGA 1 cut(s) 480
BseBI CCWGG 3 cut(s) 142, 248, 639
BseDI CCNNGG 3 cut(s) 246, 661, 691
BseGI GGATG 4 cut(s) 137, 317, 544, 634
BseJI GATNNNNATC 2 cut(s) 524, 633
BseLI CCNNNNNNNGG 3 cut(s) 147, 410, 694
BseMII CTCAG 2 cut(s) 379, 386
BseNI ACTGG 1 cut(s) 448
BsePI GCGCGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 217
BshFI GGCC 1 cut(s) 543
BshNI GGYRCC 1 cut(s) 665
BsiHKAI GWGCWC 1 cut(s) 324
BsiSI CCGG 2 cut(s) 424, 481
BslI CCNNNNNNNGG 3 cut(s) 147, 410, 694
BsmAI GTCTC 1 cut(s) 165
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 1 cut(s) 543
Bsp1286I GDGCHC 2 cut(s) 324, 661
Bsp13I TCCGGA 1 cut(s) 480
Bsp143I GATC 2 cut(s) 209, 444
Bsp19I CCATGG 1 cut(s) 661
BspANI GGCC 1 cut(s) 543
BspCNI CTCAG 2 cut(s) 378, 385
BspEI TCCGGA 1 cut(s) 480
BspFNI CGCG 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 667
BspPI GGATC 2 cut(s) 217, 439
BspT107I GGYRCC 1 cut(s) 665
BsrI ACTGG 1 cut(s) 448
BssECI CCNNGG 3 cut(s) 246, 661, 691
BssHII GCGCGC 1 cut(s) 215
BssMI GATC 2 cut(s) 209, 444
BssNI GRCGYC 1 cut(s) 336
BssT1I CCWWGG 2 cut(s) 661, 691
Bst2UI CCWGG 3 cut(s) 142, 248, 639
Bst4CI ACNGT 1 cut(s) 272
Bst6I CTCTTC 2 cut(s) 108, 425
BstACI GRCGYC 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 2 cut(s) 365, 372
BstDSI CCRYGG 1 cut(s) 661
BstF5I GGATG 4 cut(s) 137, 317, 544, 634
BstFNI CGCG 1 cut(s) 217
BstHHI GCGC 3 cut(s) 217, 219, 616
BstKTI GATC 2 cut(s) 212, 447
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 2 cut(s) 209, 444
BstNI CCWGG 3 cut(s) 142, 248, 639
BstNSI RCATGY 1 cut(s) 715
BstSCI CCNGG 4 cut(s) 140, 246, 423, 637
BstUI CGCG 1 cut(s) 217
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 444
BstYI RGATCY 1 cut(s) 444
BsuRI GGCC 1 cut(s) 543
BtgI CCRYGG 1 cut(s) 661
BtsCI GGATG 4 cut(s) 137, 317, 544, 634
Cac8I GCNNGC 1 cut(s) 217
CfoI GCGC 3 cut(s) 217, 219, 616
CseI GACGC 1 cut(s) 344
Csp6I GTAC 2 cut(s) 268, 666
CviAII CATG 5 cut(s) 223, 346, 407, 662, 712
CviJI RGCY 8 cut(s) 89, 197, 298, 414, 435, 543, 659, 720
CviKI_1 RGCY 8 cut(s) 89, 197, 298, 414, 435, 543, 659, 720
CviQI GTAC 2 cut(s) 268, 666
DdeI CTNAG 2 cut(s) 365, 372
DpnI GATC 2 cut(s) 211, 446
DpnII GATC 2 cut(s) 209, 444
DrdI GACNNNNNNGTC 1 cut(s) 389
DriI GACNNNNNGTC 1 cut(s) 348
DseDI GACNNNNNNGTC 1 cut(s) 389
Eam1104I CTCTTC 2 cut(s) 108, 425
Eam1105I GACNNNNNGTC 1 cut(s) 348
EarI CTCTTC 2 cut(s) 108, 425
Eco130I CCWWGG 2 cut(s) 661, 691
Eco24I GRGCYC 1 cut(s) 661
Eco57I CTGAAG 2 cut(s) 69, 75
EcoRII CCWGG 3 cut(s) 140, 246, 637
EcoT14I CCWWGG 2 cut(s) 661, 691
EcoT22I ATGCAT 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 661
ErhI CCWWGG 2 cut(s) 661, 691
FaeI CATG 5 cut(s) 226, 349, 410, 665, 715
FatI CATG 5 cut(s) 222, 345, 406, 661, 711
FblI GTMKAC 1 cut(s) 393
FokI GGATG 4 cut(s) 124, 324, 531, 641
FriOI GRGCYC 1 cut(s) 661
GlaI GCGC 3 cut(s) 216, 218, 615
HaeIII GGCC 1 cut(s) 543
HapII CCGG 2 cut(s) 424, 481
HgaI GACGC 1 cut(s) 344
HhaI GCGC 3 cut(s) 217, 219, 616
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 5 cut(s) 226, 349, 410, 665, 715
Hin6I GCGC 3 cut(s) 215, 217, 614
HinP1I GCGC 3 cut(s) 215, 217, 614
HincII GTYRAC 2 cut(s) 334, 353
HindII GTYRAC 2 cut(s) 334, 353
HinfI GANTC 7 cut(s) 80, 101, 107, 242, 349, 520, 594
HpaII CCGG 2 cut(s) 424, 481
Hpy166II GTNNAC 3 cut(s) 334, 353, 394
Hpy188I TCNGA 8 cut(s) 94, 106, 112, 164, 209, 375, 603, 676
Hpy188III TCNNGA 2 cut(s) 239, 481
Hpy8I GTNNAC 3 cut(s) 334, 353, 394
HpyAV CCTTC 4 cut(s) 161, 190, 533, 628
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4V TGCA 3 cut(s) 315, 624, 702
HpyF3I CTNAG 2 cut(s) 365, 372
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 5 cut(s) 226, 349, 410, 665, 715
HspAI GCGC 3 cut(s) 215, 217, 614
Kpn2I TCCGGA 1 cut(s) 480
KpnI GGTACC 1 cut(s) 669
Kzo9I GATC 2 cut(s) 209, 444
LmnI GCTCC 1 cut(s) 295
LweI GCATC 2 cut(s) 302, 667
MaeIII GTNAC 1 cut(s) 272
MalI GATC 2 cut(s) 211, 446
MboI GATC 2 cut(s) 209, 444
MflI RGATCY 1 cut(s) 444
MhlI GDGCHC 2 cut(s) 324, 661
MluCI AATT 1 cut(s) 387
MlyI GAGTC 2 cut(s) 358, 588
MmeI TCCRAC 1 cut(s) 142
Mph1103I ATGCAT 1 cut(s) 317
MroI TCCGGA 1 cut(s) 480
MseI TTAA 1 cut(s) 727
MspI CCGG 2 cut(s) 424, 481
MspR9I CCNGG 4 cut(s) 142, 248, 425, 639
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 3 cut(s) 142, 248, 639
MvnI CGCG 1 cut(s) 217
NciI CCSGG 1 cut(s) 425
NcoI CCATGG 1 cut(s) 661
NdeII GATC 2 cut(s) 209, 444
NlaIII CATG 5 cut(s) 226, 349, 410, 665, 715
NlaIV GGNNCC 1 cut(s) 667
NsiI ATGCAT 1 cut(s) 317
NspI RCATGY 1 cut(s) 715
PauI GCGCGC 1 cut(s) 215
PciI ACATGT 1 cut(s) 711
PctI GAATGC 1 cut(s) 490
PfeI GAWTC 5 cut(s) 80, 101, 107, 242, 520
PfoI TCCNGGA 2 cut(s) 423, 637
PleI GAGTC 2 cut(s) 357, 588
PpsI GAGTC 2 cut(s) 357, 588
PscI ACATGT 1 cut(s) 711
Psp6I CCWGG 3 cut(s) 140, 246, 637
PspGI CCWGG 3 cut(s) 140, 246, 637
PspN4I GGNNCC 1 cut(s) 667
PsuI RGATCY 1 cut(s) 444
PteI GCGCGC 1 cut(s) 215
RsaI GTAC 2 cut(s) 269, 667
RsaNI GTAC 2 cut(s) 268, 666
SaqAI TTAA 1 cut(s) 727
Sau3AI GATC 2 cut(s) 209, 444
SchI GAGTC 2 cut(s) 358, 588
ScrFI CCNGG 4 cut(s) 142, 248, 425, 639
SduI GDGCHC 2 cut(s) 324, 661
SetI ASST 6 cut(s) 91, 236, 300, 380, 437, 693
SfaNI GCATC 2 cut(s) 302, 667
Sse9I AATT 1 cut(s) 387
StyD4I CCNGG 4 cut(s) 140, 246, 423, 637
StyI CCWWGG 2 cut(s) 661, 691
TaaI ACNGT 1 cut(s) 272
TaqI TCGA 2 cut(s) 240, 381
TasI AATT 1 cut(s) 387
TatI WGTACW 1 cut(s) 267
TfiI GAWTC 5 cut(s) 80, 101, 107, 242, 520
Tru1I TTAA 1 cut(s) 727
Tru9I TTAA 1 cut(s) 727
TspDTI ATGAA 2 cut(s) 93, 512
TspGWI ACGGA 1 cut(s) 342
XceI RCATGY 1 cut(s) 715
XmiI GTMKAC 1 cut(s) 393
Zsp2I ATGCAT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.