RLG00000030854

Domain of unknown function (DUF2431)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016278
Physical Location & Seq
Reverse (-)
215963 .. 216875
913 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030854

Sequence Viewer

Length: 720 bp
ATGGGAAGAGAAGACGAAGAAGAAGAATACCAACAAGAAAGGGAAACTGATGATGACGACGATGATGATGATGATGAATCTTCAGAGGAAGAATCAGAATCGGAAGAGAAATGGAGAAAGCACTACTCATCCAGGCATAGGATATTGTTGGTCGGAGAAGGAGACTTCTCCTTCTCTCTGTCTTTAGCTACACACTTCGGATCGGCGCGCAACATGGTCGCCACCTCTCTCGATTCCCAGGAGAAGATATGTAGCAAGTACAGTAACGCAATAGCGAATGTGAGGGAGCTTGAAGAAAGGGGATGCTTAGTGCTCTACGGAGTTGACGCCAAGACCATGAGTCAACACTACTTCCTCAGCACTCAGAGGTTCGACAGAATTGTCTACAACTTCCCTCATGTCGGCTTTCTCTTCCGGGAGGATAGCTACTGCCAGATCCAGTTGAACAAGAGATTGGTCAAGGGTTTTCTCAGGAATGCTAAACTTCTTTTGAAAAAAGAGGGAGGGCAGATTCATATCTCTCACAAAGAAGGCCATCCTTACAACAAATGGGATTTAGTGAAGAAAGCACACAAGATTGGAGGACTCGTTCTGACCCAATCTGTGCCCTTCTGCAAGGATGACTATCCTGGATATGACAACAAGAGAGCCCATGGTACCCTTTCCGATGCCTCTTTCCACCTTGGGGATTGCACTACTTACATGTTTAGCCTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

240

Amino Acids

27.58

Weight (kDa)

5.38

Isoelectric Point (pI)

54.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 49 - 216 2.3e-54 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016278)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00200
malus_domestica MD10G1341000.v1.1
prunus_persica Prupe.4G002200_v2.0.a1
pyrus_communis pycom10g29060
rosa_chinensis RchiOBHm_Chr5g0000261
rosa_laevigata RLG00000030854
rosa_multiflora Rmu_sc0043092.1_g000001 Rmu_ssc0000385.1_g000003
rosa_roxburghii Rroxscaffold_1G00075840
rosa_rugosa Rorug04G0382900
rosa_samantha Rh5AG001900 Rh5BG003300 Rh5CG002200 Rh5DG002200
rosa_wichuraiana Rw5G000240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 380
Acc65I GGTACC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 656
AccI GTMKAC 1 cut(s) 384
AccII CGCG 1 cut(s) 208
AclWI GGATC 2 cut(s) 208, 430
AcuI CTGAAG 1 cut(s) 66
AcyI GRCGYC 1 cut(s) 327
AfaI GTAC 2 cut(s) 260, 658
AfiI CCNNNNNNNGG 3 cut(s) 138, 401, 685
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 3 cut(s) 293, 445, 493
AhdI GACNNNNNGTC 1 cut(s) 339
AjnI CCWGG 3 cut(s) 131, 237, 628
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AloI GAACNNNNNNTCC 2 cut(s) 573, 605
AluBI AGCT 3 cut(s) 188, 289, 426
AluI AGCT 3 cut(s) 188, 289, 426
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 2 cut(s) 208, 430
AoxI GGCC 1 cut(s) 532
Asp718I GGTACC 1 cut(s) 656
AspLEI GCGC 2 cut(s) 208, 210
AsuC2I CCSGG 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 609
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 1 cut(s) 652
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 315
BbvCI CCTCAGC 1 cut(s) 356
BccI CCATC 1 cut(s) 543
BcgI CGANNNNNNTGC 2 cut(s) 199, 233
BciT130I CCWGG 3 cut(s) 133, 239, 630
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 1 cut(s) 156
Bme1390I CCNGG 4 cut(s) 133, 239, 416, 630
BmeRI GACNNNNNGTC 1 cut(s) 339
BmiI GGNNCC 1 cut(s) 658
BmrFI CCNGG 4 cut(s) 133, 239, 416, 630
BmsI GCATC 2 cut(s) 293, 658
BpiI GAAGAC 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 416
BsaBI GATNNNNATC 2 cut(s) 515, 624
BsaHI GRCGYC 1 cut(s) 327
BsaJI CCNNGG 3 cut(s) 237, 652, 682
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 401, 685
Bse1I ACTGG 1 cut(s) 439
Bse8I GATNNNNATC 2 cut(s) 515, 624
BseBI CCWGG 3 cut(s) 133, 239, 630
BseDI CCNNGG 3 cut(s) 237, 652, 682
BseGI GGATG 4 cut(s) 128, 308, 535, 625
BseJI GATNNNNATC 2 cut(s) 515, 624
BseLI CCNNNNNNNGG 3 cut(s) 138, 401, 685
BseMII CTCAG 3 cut(s) 370, 377, 484
BseNI ACTGG 1 cut(s) 439
BsePI GCGCGC 1 cut(s) 206
BseSI GKGCMC 1 cut(s) 609
Bsh1236I CGCG 1 cut(s) 208
BshFI GGCC 1 cut(s) 534
BshNI GGYRCC 1 cut(s) 656
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 1 cut(s) 415
BslI CCNNNNNNNGG 3 cut(s) 138, 401, 685
BsmAI GTCTC 1 cut(s) 156
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 1 cut(s) 534
Bsp1286I GDGCHC 3 cut(s) 315, 609, 652
Bsp143I GATC 2 cut(s) 200, 435
Bsp19I CCATGG 1 cut(s) 652
BspANI GGCC 1 cut(s) 534
BspCNI CTCAG 3 cut(s) 369, 376, 483
BspFNI CGCG 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 658
BspPI GGATC 2 cut(s) 208, 430
BspT107I GGYRCC 1 cut(s) 656
BsrI ACTGG 1 cut(s) 439
BssECI CCNNGG 3 cut(s) 237, 652, 682
BssHII GCGCGC 1 cut(s) 206
BssMI GATC 2 cut(s) 200, 435
BssNI GRCGYC 1 cut(s) 327
BssT1I CCWWGG 2 cut(s) 652, 682
Bst2UI CCWGG 3 cut(s) 133, 239, 630
Bst4CI ACNGT 1 cut(s) 263
Bst6I CTCTTC 2 cut(s) 99, 416
BstACI GRCGYC 1 cut(s) 327
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 4 cut(s) 307, 356, 363, 470
BstDSI CCRYGG 1 cut(s) 652
BstF5I GGATG 4 cut(s) 128, 308, 535, 625
BstFNI CGCG 1 cut(s) 208
BstHHI GCGC 2 cut(s) 208, 210
BstKTI GATC 2 cut(s) 203, 438
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 2 cut(s) 200, 435
BstNI CCWGG 3 cut(s) 133, 239, 630
BstNSI RCATGY 1 cut(s) 706
BstSCI CCNGG 4 cut(s) 131, 237, 414, 628
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 1 cut(s) 208
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 435
BstYI RGATCY 1 cut(s) 435
BsuRI GGCC 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 652
BtsCI GGATG 4 cut(s) 128, 308, 535, 625
Cac8I GCNNGC 1 cut(s) 208
CfoI GCGC 2 cut(s) 208, 210
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 259, 657
CviAII CATG 5 cut(s) 214, 337, 398, 653, 703
CviJI RGCY 7 cut(s) 188, 289, 405, 426, 534, 650, 711
CviKI_1 RGCY 7 cut(s) 188, 289, 405, 426, 534, 650, 711
CviQI GTAC 2 cut(s) 259, 657
DdeI CTNAG 4 cut(s) 307, 356, 363, 470
DpnI GATC 2 cut(s) 202, 437
DpnII GATC 2 cut(s) 200, 435
DrdI GACNNNNNNGTC 1 cut(s) 380
DriI GACNNNNNGTC 1 cut(s) 339
DseDI GACNNNNNNGTC 1 cut(s) 380
Eam1104I CTCTTC 2 cut(s) 99, 416
Eam1105I GACNNNNNGTC 1 cut(s) 339
EarI CTCTTC 2 cut(s) 99, 416
Eco130I CCWWGG 2 cut(s) 652, 682
Eco24I GRGCYC 1 cut(s) 652
Eco57I CTGAAG 1 cut(s) 66
EcoRII CCWGG 3 cut(s) 131, 237, 628
EcoT14I CCWWGG 2 cut(s) 652, 682
EcoT38I GRGCYC 1 cut(s) 652
ErhI CCWWGG 2 cut(s) 652, 682
FaeI CATG 5 cut(s) 217, 340, 401, 656, 706
FaiI YATR 9 cut(s) 138, 215, 250, 338, 399, 516, 636, 654, 704
FatI CATG 5 cut(s) 213, 336, 397, 652, 702
FblI GTMKAC 1 cut(s) 384
FokI GGATG 4 cut(s) 115, 315, 522, 632
FriOI GRGCYC 1 cut(s) 652
GlaI GCGC 2 cut(s) 207, 209
HaeIII GGCC 1 cut(s) 534
HapII CCGG 1 cut(s) 415
HgaI GACGC 1 cut(s) 335
HhaI GCGC 2 cut(s) 208, 210
Hin1I GRCGYC 1 cut(s) 327
Hin1II CATG 5 cut(s) 217, 340, 401, 656, 706
Hin6I GCGC 2 cut(s) 206, 208
HinP1I GCGC 2 cut(s) 206, 208
HincII GTYRAC 2 cut(s) 325, 344
HindII GTYRAC 2 cut(s) 325, 344
HinfI GANTC 7 cut(s) 77, 92, 98, 233, 340, 511, 585
HpaII CCGG 1 cut(s) 415
Hpy166II GTNNAC 3 cut(s) 325, 344, 385
Hpy188I TCNGA 8 cut(s) 85, 97, 103, 155, 200, 366, 594, 667
Hpy188III TCNNGA 2 cut(s) 230, 472
Hpy8I GTNNAC 3 cut(s) 325, 344, 385
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 4 cut(s) 152, 181, 524, 619
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4V TGCA 2 cut(s) 615, 693
HpyF3I CTNAG 4 cut(s) 307, 356, 363, 470
Hsp92I GRCGYC 1 cut(s) 327
Hsp92II CATG 5 cut(s) 217, 340, 401, 656, 706
HspAI GCGC 2 cut(s) 206, 208
KpnI GGTACC 1 cut(s) 660
Kzo9I GATC 2 cut(s) 200, 435
LmnI GCTCC 1 cut(s) 286
LweI GCATC 2 cut(s) 293, 658
MaeIII GTNAC 1 cut(s) 263
MalI GATC 2 cut(s) 202, 437
MboI GATC 2 cut(s) 200, 435
MflI RGATCY 1 cut(s) 435
MhlI GDGCHC 3 cut(s) 315, 609, 652
MluCI AATT 1 cut(s) 378
MlyI GAGTC 2 cut(s) 349, 579
MmeI TCCRAC 1 cut(s) 133
MseI TTAA 1 cut(s) 718
MspI CCGG 1 cut(s) 415
MspR9I CCNGG 4 cut(s) 133, 239, 416, 630
Mva1269I GAATGC 1 cut(s) 481
MvaI CCWGG 3 cut(s) 133, 239, 630
MvnI CGCG 1 cut(s) 208
NciI CCSGG 1 cut(s) 416
NcoI CCATGG 1 cut(s) 652
NdeII GATC 2 cut(s) 200, 435
NlaIII CATG 5 cut(s) 217, 340, 401, 656, 706
NlaIV GGNNCC 1 cut(s) 658
NspI RCATGY 1 cut(s) 706
PauI GCGCGC 1 cut(s) 206
PciI ACATGT 1 cut(s) 702
PctI GAATGC 1 cut(s) 481
PfeI GAWTC 5 cut(s) 77, 92, 98, 233, 511
PfoI TCCNGGA 2 cut(s) 414, 628
PleI GAGTC 2 cut(s) 348, 579
PpsI GAGTC 2 cut(s) 348, 579
PscI ACATGT 1 cut(s) 702
Psp6I CCWGG 3 cut(s) 131, 237, 628
PspGI CCWGG 3 cut(s) 131, 237, 628
PspN4I GGNNCC 1 cut(s) 658
PsuI RGATCY 1 cut(s) 435
PteI GCGCGC 1 cut(s) 206
RsaI GTAC 2 cut(s) 260, 658
RsaNI GTAC 2 cut(s) 259, 657
SaqAI TTAA 1 cut(s) 718
Sau3AI GATC 2 cut(s) 200, 435
SchI GAGTC 2 cut(s) 349, 579
ScrFI CCNGG 4 cut(s) 133, 239, 416, 630
SduI GDGCHC 3 cut(s) 315, 609, 652
SetI ASST 6 cut(s) 190, 227, 291, 371, 428, 684
SfaNI GCATC 2 cut(s) 293, 658
Sse9I AATT 1 cut(s) 378
StyD4I CCNGG 4 cut(s) 131, 237, 414, 628
StyI CCWWGG 2 cut(s) 652, 682
TaaI ACNGT 1 cut(s) 263
TaqI TCGA 2 cut(s) 231, 372
TasI AATT 1 cut(s) 378
TatI WGTACW 1 cut(s) 258
TfiI GAWTC 5 cut(s) 77, 92, 98, 233, 511
Tru1I TTAA 1 cut(s) 718
Tru9I TTAA 1 cut(s) 718
TspDTI ATGAA 2 cut(s) 90, 503
TspGWI ACGGA 1 cut(s) 333
XceI RCATGY 1 cut(s) 706
XmiI GTMKAC 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.