MD11G1024300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0014707
Physical Location & Seq
Reverse (-)
2112148 .. 2114771
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1024300.v1.1.491

Sequence Viewer

Length: 1620 bp
ATGAAAATACCATCTTTTTCGACATTCAAGTTGTCTCCAGACGACTTGGCTTTGCTTTTACAGAAATGCATGAAAGCAAGGGCTCTGAGACAAGGCAAGCAAATTCATGCAGTGCTGTTAACAAGTGGGGTGGACATGAATTTGTTATCTTTGAGTTCAAAGCTTGTGGGGATGTATGCTGGTTGTGGGGATGTTGGGAATGCACACCAAGTGTTTGATAAAATTCCCAAGCCAAACGTCTTCGCGTTGAACTGGCTGGTTTTGGCGTCAGCGTTTAATGGGAAGTTTGAGGATGCAATTGGGTACTTTTATTTGATGCAAGAGTTGGGAATTGTTGGCAACAAGTTCACGTTTCCTGTCGTGCTTAAGGTTTGTGTTGGTTTGATGGATTTGAACAAGGGAAAGGAAGTTCATGCTGTGGTGAATAAACTTGGTTTTGAAAAGGATGTGTCTTTGGCAAATGCCTTGATTGACATGTATTATAAATGTGGAAGTTTGTGTTATGCTCGTCGCGTGTTTGATAGGATGCTCGACAGAGACGTTGTTTCTTGGACGTCGATGATATGCGGGTATTTTAACATAGGAAAGACTGATCAAGCGCTCCTCTTGTTTGAGAGGATGAGGTTGGATGGATTGGAACCGAATGATTTCACATGGAATGCAATGATAGCGGGGTTTGCTCGGTGTGGAGATACAAATCGAGCATATGCGCTTCTGTCTAGGATGACTAAAGAGGGATTGGTTCCTGATTTTGTTACTTGGAATGCGTTGATTTCAGGTTTTACCCAAGGCCAGCATGCTGATGAAGCATTTAAACTGTTCAGGATGATGTTGGTGTCTGGAATTAAAGCGAACCTCGTGACTATCACAGGGTTGCTACCAGCTTGTGGGATCATTGGTTCAATTCAAAGAGGGAGAGAAATTCATGGATTGATCTACAGGATGGGCTTTGACGTAAATGTGTTTGTTGCTAGTGCTCTTATTGATATGTACTCAAAATGTGGCAGTGTGAAAAATGCTCGAAGTGTATTTGACCAGAATCCTGTAAATAACATTGCATCATGGAATGCATTGATTGGATGTTATGGTAAGTACGGTATGGTTGATTCCTCCATAGAGCTTTTTGAAAGAATGCAAGCAGAAGGAGTGCAGGCAAATGAAGTGACTTTGACATCCGTACTTTCTGCGTGCAGCCACAGTGGTTATGTGGAGAAAGGTTTAAAGATTTTCAAGTCCATGAAAATGTGTCATGGAGTTGAGCCAGGTACAGAACATTATGCTTGTGTTGTTGATCTCTTGTGTCGGTCAGGAAAGATGGTAGCAGCTTACGAATTGGTTAAGGGAATGCCTATAGAAGTCACTGAATCTATCATTGGAGCCTTTTTCAACGGTTGCAAAGTCCATGGAAGAAGAGATCTTGCTAAAATGATGGCAGAAGCTATTCTAAAAATGGAGTTAAAGAGACCTGGAGGTTTTGTAACGCTTTCGAATATCCATGCTGCTAACGGAGAATGGGAAGAGGTTGAGAACGTAAGGATGGTGATGAAGCAGAGGAAGATTCTTAAGCAACCTGGATTTAGTTGGCTCGGTAAAAAGATGGCTTTGTCAGACTTGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

540

Amino Acids

59.67

Weight (kDa)

8.87

Isoelectric Point (pI)

32.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 179 - 228 1.7e-13 PPR repeat family
PPR_3 PF13812 180 - 224 5.2e-06 Pentatricopeptide repeat domain
PPR PF01535 182 - 212 2.9e-06 PPR repeat
PPR_long PF17177 198 - 304 6.2e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 210 - 243 3.7e-08 PPR repeat
PPR_2 PF13041 214 - 261 5.2e-12 PPR repeat family
PPR PF01535 217 - 247 2.1e-06 PPR repeat
PPR_1 PF12854 246 - 277 1e-07 PPR repeat
PPR_2 PF13041 249 - 296 7.7e-10 PPR repeat family
PPR PF01535 252 - 282 2.7e-06 PPR repeat
PPR_2 PF13041 351 - 398 4.4e-10 PPR repeat family
PPR PF01535 354 - 383 3.1e-07 PPR repeat
PPR_long PF17177 367 - 465 1.5e-07 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 472 - 529 7.8e-13 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014707)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43470
malus_domestica MD11G1024300.v1.1
prunus_persica Prupe.6G020300_v2.0.a1
pyrus_communis pycom11g01920
rosa_chinensis RchiOBHm_Chr5g0077881
rosa_laevigata RLG00000036722
rosa_multiflora Rmu_co8015760.1_g000001 Rmu_sc0031428.1_g000002 Rmu_ssc0000186.1_g000002
rosa_roxburghii Rroxscaffold_1G00004300
rosa_rugosa Rorug05G0456900 Rorug05G0456900
rosa_samantha Rh5AG511300 Rh5BG534000 Rh5CG557500 Rh5DG545500
rosa_wichuraiana Rw5G047450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 483
AatII GACGTC 1 cut(s) 557
AccB7I CCANNNNNTGG 1 cut(s) 887
AccII CGCG 2 cut(s) 245, 513
AciI CCGC 2 cut(s) 567, 671
AclWI GGATC 1 cut(s) 899
AcsI RAATTY 4 cut(s) 102, 139, 222, 921
AcyI GRCGYC 2 cut(s) 266, 554
AdeI CACNNNGTG 1 cut(s) 211
AfaI GTAC 5 cut(s) 305, 992, 1094, 1179, 1267
AfeI AGCGCT 1 cut(s) 600
AfiI CCNNNNNNNGG 1 cut(s) 887
AflII CTTAAG 2 cut(s) 365, 1562
AflIII ACRYGT 1 cut(s) 474
AjnI CCWGG 3 cut(s) 1261, 1465, 1570
AjuI GAANNNNNNNTTGG 2 cut(s) 1356, 1388
AloI GAACNNNNNNTCC 4 cut(s) 393, 425, 883, 915
AluBI AGCT 5 cut(s) 163, 884, 1120, 1325, 1439
AluI AGCT 5 cut(s) 163, 884, 1120, 1325, 1439
Alw21I GWGCWC 1 cut(s) 979
Alw26I GTCTC 4 cut(s) 39, 82, 531, 1456
AlwI GGATC 1 cut(s) 899
Aor51HI AGCGCT 1 cut(s) 600
AoxI GGCC 1 cut(s) 790
ApeKI GCWGC 3 cut(s) 1191, 1322, 1499
ApoI RAATTY 4 cut(s) 102, 139, 222, 921
Asp700I GAANNNNTTC 2 cut(s) 647, 1440
AspLEI GCGC 2 cut(s) 601, 712
AsuHPI GGTGA 2 cut(s) 433, 1552
AsuII TTCGAA 1 cut(s) 1487
BanII GRGCYC 1 cut(s) 85
BauI CACGAG 1 cut(s) 857
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 1 cut(s) 979
BbvI GCAGC 3 cut(s) 1203, 1334, 1486
BccI CCATC 8 cut(s) 19, 379, 623, 937, 1309, 1423, 1531, 1591
BciT130I CCWGG 3 cut(s) 1263, 1467, 1572
BclI TGATCA 1 cut(s) 592
BcoDI GTCTC 4 cut(s) 39, 82, 531, 1456
BfaI CTAG 2 cut(s) 720, 972
BfmI CTRYAG 2 cut(s) 937, 1350
BfoI RGCGCY 1 cut(s) 602
BfrI CTTAAG 2 cut(s) 365, 1562
BglII AGATCT 1 cut(s) 1414
BisI GCNGC 3 cut(s) 1192, 1323, 1500
BlsI GCNGC 3 cut(s) 1193, 1324, 1501
Bme1390I CCNGG 3 cut(s) 1263, 1467, 1572
BmiI GGNNCC 3 cut(s) 639, 744, 1378
BmrFI CCNGG 3 cut(s) 1263, 1467, 1572
BmsI GCATC 4 cut(s) 283, 306, 516, 1067
BpiI GAAGAC 1 cut(s) 232
BpmI CTGGAG 2 cut(s) 21, 1488
Bpu14I TTCGAA 1 cut(s) 1487
BsaBI GATNNNNATC 1 cut(s) 696
BsaHI GRCGYC 2 cut(s) 266, 554
BsaI GGTCTC 1 cut(s) 1456
BsaJI CCNNGG 2 cut(s) 787, 1402
BsaXI ACNNNNNCTCC 2 cut(s) 1461, 1491
Bsc4I CCNNNNNNNGG 1 cut(s) 887
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 2 cut(s) 669, 1053
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 3 cut(s) 1263, 1467, 1572
BseDI CCNNGG 2 cut(s) 787, 1402
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 1 cut(s) 887
BseMI GCAATG 2 cut(s) 669, 1053
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 1 cut(s) 593
BseXI GCAGC 3 cut(s) 1203, 1334, 1486
BsgI GTGCAG 2 cut(s) 1169, 1210
Bsh1236I CGCG 2 cut(s) 245, 513
BshFI GGCC 1 cut(s) 792
BsiHKAI GWGCWC 1 cut(s) 979
BslI CCNNNNNNNGG 1 cut(s) 887
BsmAI GTCTC 4 cut(s) 39, 82, 531, 1456
BsmBI CGTCTC 1 cut(s) 531
BsmI GAATGC 6 cut(s) 205, 664, 769, 1072, 1137, 1350
BsnI GGCC 1 cut(s) 792
Bso31I GGTCTC 1 cut(s) 1456
Bsp119I TTCGAA 1 cut(s) 1487
Bsp1286I GDGCHC 2 cut(s) 85, 979
Bsp143I GATC 5 cut(s) 592, 891, 933, 1291, 1414
Bsp19I CCATGG 1 cut(s) 1402
BspACI CCGC 2 cut(s) 567, 671
BspANI GGCC 1 cut(s) 792
BspCNI CTCAG 1 cut(s) 78
BspFNI CGCG 2 cut(s) 245, 513
BspLI GGNNCC 3 cut(s) 639, 744, 1378
BspPI GGATC 1 cut(s) 899
BspT104I TTCGAA 1 cut(s) 1487
BspTI CTTAAG 2 cut(s) 365, 1562
BspTNI GGTCTC 1 cut(s) 1456
BsrDI GCAATG 2 cut(s) 669, 1053
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 787, 1402
BssMI GATC 5 cut(s) 592, 891, 933, 1291, 1414
BssNI GRCGYC 2 cut(s) 266, 554
BssSI CACGAG 1 cut(s) 857
BssT1I CCWWGG 2 cut(s) 787, 1402
Bst2BI CACGAG 1 cut(s) 857
Bst2UI CCWGG 3 cut(s) 1263, 1467, 1572
Bst4CI ACNGT 4 cut(s) 819, 1097, 1199, 1391
Bst6I CTCTTC 2 cut(s) 1405, 1512
BstACI GRCGYC 2 cut(s) 266, 554
BstAFI CTTAAG 2 cut(s) 365, 1562
BstBI TTCGAA 1 cut(s) 1487
BstC8I GCNNGC 6 cut(s) 98, 794, 798, 1137, 1152, 1189
BstDEI CTNAG 1 cut(s) 86
BstDSI CCRYGG 1 cut(s) 1402
BstFNI CGCG 2 cut(s) 245, 513
BstH2I RGCGCY 1 cut(s) 602
BstHHI GCGC 2 cut(s) 601, 712
BstKTI GATC 5 cut(s) 595, 894, 936, 1294, 1417
BstMAI GTCTC 4 cut(s) 39, 82, 531, 1456
BstMBI GATC 5 cut(s) 592, 891, 933, 1291, 1414
BstMWI GCNNNNNNNGC 3 cut(s) 668, 677, 806
BstNI CCWGG 3 cut(s) 1263, 1467, 1572
BstNSI RCATGY 2 cut(s) 478, 800
BstSCI CCNGG 3 cut(s) 1261, 1465, 1570
BstSFI CTRYAG 2 cut(s) 937, 1350
BstUI CGCG 2 cut(s) 245, 513
BstV1I GCAGC 3 cut(s) 1203, 1334, 1486
BstV2I GAAGAC 1 cut(s) 232
BstX2I RGATCY 1 cut(s) 1414
BstYI RGATCY 1 cut(s) 1414
BsuRI GGCC 1 cut(s) 792
BtgI CCRYGG 1 cut(s) 1402
BtsI GCAGTG 2 cut(s) 117, 1012
BtsIMutI CAGTG 4 cut(s) 117, 1012, 1204, 1359
Cac8I GCNNGC 6 cut(s) 98, 794, 798, 1137, 1152, 1189
CfoI GCGC 2 cut(s) 601, 712
CseI GACGC 1 cut(s) 255
Csp6I GTAC 5 cut(s) 304, 991, 1093, 1178, 1266
CspCI CAANNNNNGTGG 4 cut(s) 111, 146, 147, 182
CviQI GTAC 5 cut(s) 304, 991, 1093, 1178, 1266
DdeI CTNAG 1 cut(s) 86
DpnI GATC 5 cut(s) 594, 893, 935, 1293, 1416
DpnII GATC 5 cut(s) 592, 891, 933, 1291, 1414
DraI TTTAAA 2 cut(s) 814, 1221
DraIII CACNNNGTG 1 cut(s) 211
Eam1104I CTCTTC 2 cut(s) 1405, 1512
EarI CTCTTC 2 cut(s) 1405, 1512
Eco130I CCWWGG 2 cut(s) 787, 1402
Eco24I GRGCYC 1 cut(s) 85
Eco31I GGTCTC 1 cut(s) 1456
Eco47III AGCGCT 1 cut(s) 600
EcoRII CCWGG 3 cut(s) 1261, 1465, 1570
EcoT14I CCWWGG 2 cut(s) 787, 1402
EcoT22I ATGCAT 2 cut(s) 71, 1072
EcoT38I GRGCYC 1 cut(s) 85
ErhI CCWWGG 2 cut(s) 787, 1402
Esp3I CGTCTC 1 cut(s) 531
FauI CCCGC 2 cut(s) 560, 664
FauNDI CATATG 1 cut(s) 706
FbaI TGATCA 1 cut(s) 592
Fnu4HI GCNGC 3 cut(s) 1192, 1323, 1500
FriOI GRGCYC 1 cut(s) 85
Fsp4HI GCNGC 3 cut(s) 1192, 1323, 1500
FspBI CTAG 2 cut(s) 720, 972
GlaI GCGC 2 cut(s) 600, 711
GluI GCNGC 3 cut(s) 1192, 1323, 1500
GsuI CTGGAG 2 cut(s) 21, 1488
HaeII RGCGCY 1 cut(s) 602
HaeIII GGCC 1 cut(s) 792
HgaI GACGC 1 cut(s) 255
HhaI GCGC 2 cut(s) 601, 712
Hin1I GRCGYC 2 cut(s) 266, 554
Hin6I GCGC 2 cut(s) 599, 710
HinP1I GCGC 2 cut(s) 599, 710
HincII GTYRAC 1 cut(s) 120
HindII GTYRAC 1 cut(s) 120
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 4 cut(s) 1039, 1106, 1364, 1558
HpaI GTTAAC 1 cut(s) 120
HphI GGTGA 2 cut(s) 433, 1552
Hpy166II GTNNAC 3 cut(s) 120, 133, 348
Hpy188I TCNGA 2 cut(s) 87, 1609
Hpy188III TCNNGA 6 cut(s) 38, 746, 823, 840, 859, 1308
Hpy8I GTNNAC 3 cut(s) 120, 133, 348
Hpy99I CGWCG 2 cut(s) 513, 559
HpyAV CCTTC 1 cut(s) 1136
HpyCH4III ACNGT 4 cut(s) 819, 1097, 1199, 1391
HpyCH4IV ACGT 6 cut(s) 237, 350, 540, 554, 954, 1530
HpyF10VI GCNNNNNNNGC 3 cut(s) 668, 677, 806
HpyF3I CTNAG 1 cut(s) 86
HpySE526I ACGT 6 cut(s) 237, 350, 540, 554, 954, 1530
Hsp92I GRCGYC 2 cut(s) 266, 554
HspAI GCGC 2 cut(s) 599, 710
Ksp22I TGATCA 1 cut(s) 592
KspAI GTTAAC 1 cut(s) 120
Kzo9I GATC 5 cut(s) 592, 891, 933, 1291, 1414
LmnI GCTCC 2 cut(s) 606, 1376
Lsp1109I GCAGC 3 cut(s) 1203, 1334, 1486
LweI GCATC 4 cut(s) 283, 306, 516, 1067
MaeI CTAG 2 cut(s) 720, 972
MaeII ACGT 6 cut(s) 237, 350, 540, 554, 954, 1530
MaeIII GTNAC 5 cut(s) 754, 859, 1162, 1357, 1477
MalI GATC 5 cut(s) 594, 893, 935, 1293, 1416
MboI GATC 5 cut(s) 592, 891, 933, 1291, 1414
MboII GAAGA 5 cut(s) 232, 1419, 1422, 1529, 1567
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 1 cut(s) 1414
MhlI GDGCHC 2 cut(s) 85, 979
MluCI AATT 9 cut(s) 102, 139, 222, 297, 330, 843, 903, 921, 1331
MmeI TCCRAC 1 cut(s) 606
Mph1103I ATGCAT 2 cut(s) 71, 1072
MroXI GAANNNNTTC 2 cut(s) 647, 1440
MslI CAYNNNNRTG 2 cut(s) 801, 1241
MspCI CTTAAG 2 cut(s) 365, 1562
MspR9I CCNGG 3 cut(s) 1263, 1467, 1572
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 6 cut(s) 205, 664, 769, 1072, 1137, 1350
MvaI CCWGG 3 cut(s) 1263, 1467, 1572
MvnI CGCG 2 cut(s) 245, 513
MwoI GCNNNNNNNGC 3 cut(s) 668, 677, 806
NcoI CCATGG 1 cut(s) 1402
NdeI CATATG 1 cut(s) 706
NdeII GATC 5 cut(s) 592, 891, 933, 1291, 1414
NlaIV GGNNCC 3 cut(s) 639, 744, 1378
NmuCI GTSAC 3 cut(s) 859, 1162, 1357
NsiI ATGCAT 2 cut(s) 71, 1072
NspI RCATGY 2 cut(s) 478, 800
NspV TTCGAA 1 cut(s) 1487
PaeI GCATGC 1 cut(s) 800
PciI ACATGT 1 cut(s) 474
PcsI WCGNNNNNNNCGW 1 cut(s) 537
PctI GAATGC 6 cut(s) 205, 664, 769, 1072, 1137, 1350
PdmI GAANNNNTTC 2 cut(s) 647, 1440
PfeI GAWTC 4 cut(s) 1039, 1106, 1364, 1558
PflMI CCANNNNNTGG 1 cut(s) 887
PkrI GCNGC 3 cut(s) 1193, 1324, 1501
PscI ACATGT 1 cut(s) 474
PsiI TTATAA 1 cut(s) 483
Psp6I CCWGG 3 cut(s) 1261, 1465, 1570
PspGI CCWGG 3 cut(s) 1261, 1465, 1570
PspN4I GGNNCC 3 cut(s) 639, 744, 1378
PsuI RGATCY 1 cut(s) 1414
RsaI GTAC 5 cut(s) 305, 992, 1094, 1179, 1267
RsaNI GTAC 5 cut(s) 304, 991, 1093, 1178, 1266
RseI CAYNNNNRTG 2 cut(s) 801, 1241
SatI GCNGC 3 cut(s) 1192, 1323, 1500
Sau3AI GATC 5 cut(s) 592, 891, 933, 1291, 1414
ScrFI CCNGG 3 cut(s) 1263, 1467, 1572
SduI GDGCHC 2 cut(s) 85, 979
SfaNI GCATC 4 cut(s) 283, 306, 516, 1067
SfcI CTRYAG 2 cut(s) 937, 1350
SfuI TTCGAA 1 cut(s) 1487
SmiMI CAYNNNNRTG 2 cut(s) 801, 1241
SmlI CTYRAG 2 cut(s) 365, 1562
SmoI CTYRAG 2 cut(s) 365, 1562
SphI GCATGC 1 cut(s) 800
Sse9I AATT 9 cut(s) 102, 139, 222, 297, 330, 843, 903, 921, 1331
SsiI CCGC 2 cut(s) 567, 671
SspMI CTAG 2 cut(s) 720, 972
StyD4I CCNGG 3 cut(s) 1261, 1465, 1570
StyI CCWWGG 2 cut(s) 787, 1402
TaaI ACNGT 4 cut(s) 819, 1097, 1199, 1391
TaiI ACGT 6 cut(s) 240, 353, 543, 557, 957, 1533
TaqI TCGA 6 cut(s) 20, 531, 557, 700, 1021, 1487
TaqII GACCGA 1 cut(s) 1293
TasI AATT 9 cut(s) 102, 139, 222, 297, 330, 843, 903, 921, 1331
TatI WGTACW 1 cut(s) 990
TfiI GAWTC 4 cut(s) 1039, 1106, 1364, 1558
TscAI CASTG 4 cut(s) 117, 1012, 1204, 1366
TseFI GTSAC 3 cut(s) 859, 1162, 1357
TseI GCWGC 3 cut(s) 1191, 1322, 1499
Tsp45I GTSAC 3 cut(s) 859, 1162, 1357
TspGWI ACGGA 2 cut(s) 1165, 1521
TspRI CASTG 4 cut(s) 117, 1012, 1204, 1366
Van91I CCANNNNNTGG 1 cut(s) 887
Vha464I CTTAAG 2 cut(s) 365, 1562
XapI RAATTY 4 cut(s) 102, 139, 222, 921
XceI RCATGY 2 cut(s) 478, 800
XmnI GAANNNNTTC 2 cut(s) 647, 1440
XspI CTAG 2 cut(s) 720, 972
ZraI GACGTC 1 cut(s) 555
Zsp2I ATGCAT 2 cut(s) 71, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.