Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0011008

Physical Location & Seq

Genomic Coordinates Reverse (-)

20930789 .. 20940013

Gene Structure 9225 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1176500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4164 bp

ATGCCGTTCTTATCGGACACCGCCAGCGCCATCAAGACCCGCTTCGGGTTCCACGACCACTCTTCAGACCCGGTCCGGAGCACTCCCAATCTCCACAAATCCGCGTCCAAAGAGAGTTTCTCGGCGCTACGGAGCATCGGAGACTGGGACGACGATGATGACGACGGAAAAGCCAGTACCGCTACGACTTCCTCAAACCAAAGCTTCGAGTTTCATGAAGACCCTTCCTTTTGGAAGGACCACAACGTGCAGGTTATTATTCGGGTTCGTCCGCTTAGCAGCGCTGAAATATCAGTGCAAGGTTATGGCAAATGCGTTCGACAAGAGAGCTGTCAGACAATTACTTGGACTGGCCATCCAGAGTCACGGTTTACTTTTGACATTGTTGCAGATGAGAATGTTAGCCAGGAGCAATTGTTCAAAGTGGCCGGATTGCCGATGGTGGACAACTGCATGATAGGCTATAATAGCTGCATGTTTGCTTATGGCCAAACTGGAAGTGGGAAGACCCACACAATGCTTGGAGATATTGAGGGAGGCACTCGAAGGCACAGTGCCAATTGCGGGATGACACCTAGAGTGTTTGAATACTTGTTCTCGAGAATTCAAAAGGAAAAAGAGGCTGGCCGAGATGAGAAATTAAAGTTTATATGTAAATGTTCTTTTTTGGAAATATACAATGAACAAATTTTGGACCTTTTGGACCCTTCATCTAACAACTTGCAGATCAGAGAAGACATAAAGAAAGGAGTTTATGTGGAAAATCTCAAAGAGGTTGAAGTGACAAGTGCTCGAGATGTTATCCAACAACTTATTCAAGGTGCAGCTAACAGAAAGGTAGCTGCTACTAATATGAATCATGCTAGCAGTCGGTCTCATAGTGTATTTACATGCATCATTGAGAGTAAAAGGGAATGCCAAGGAGTAGCTCACCATCGTTTTGCTCGGCTTAATCTTGTTGACTTAGCAGGATCTGAAAGGCAAAAGAGCTCTGGAGCTGAAGGTGAGCGTCTAAAGGAAGCAACAAATATTAACAAGTCTCTTTCAACGTTGGGACTTGTGATTATGAATCTGGTGAACATGTCAAATGGGAAGTCACTCCATGTTCCATACAGGGATTCAAAGCTTACATTTTTGCTTCAGGATTCTTTAGGAGGGAATTCAAAGACAACTATAATAGCAAATATTAGCCCTTCTAGCTGCTGTTCGTTGGAGACACTGAGCACTTTGAAGTTTGCACAGCGCGCTAAATTCATTAAGAACAATGCAATTGTGAATGAGGACGCATCTGGAGATGTCATTGCCATGAGGGTGCAAATTCAACAACTCAAGAAAGAAGTATCCCACTTGCGAGGACTGGTCAATGGAGGAACTGGAAATCAGGACAATGATATGTTAGCTGTGAGCTTTCCAGGATCTCCAGGATCTTTTAAGTGGGAAGGACCTAATGGATCATTTAGTCCATTCACTTCTAGAAAGAGGACATCTCAGAAAAAAGACTATGAAGTTGCCCTTGTTGGGGCTTTCAGGAGGGAAAAGGACAAAGATATTGCATTGCAGACGTTGGCTGCGGAAAGCCAGGCAGCTTTGCAGCTGGCCAAACAAAGAGAGGATGAAATACAAGGACTGAAGATGAGGCTACGGTTTCGAGAAGCTGGAATAAAAAGGTTGGAAGCGGTTGCCTGTGGAAAGATCTCAGCTGAGACACACTTGCTAAAAGAAAAGGAGGAGTATTTGAAAGAAATTGAGGTCCTACGAGCCCAAGTTGATCGTAACCAAGAAGTGACCAGGTTCGCAATGGAAAATTTGCGGCTGAAGGAGGAAATCAGAAGGTTGAAGTCATTTTACGAGGAAGGTGAACGAGATATAATGAATGAACAGATCATGGTGTTACAAAACAAGTTATTAGAAGCTCTCGACTGGAAACTCATGCATGAGTCAGAGCCCTCGACAGTTCAGGATAGAAATCAAGATGTGGTGATGGAAGGTCAAAATGATGATAATATGCTGATTTCCAATCAGGAACAAGCATCACCTTGGCAGTCTTCAATTAAGGAGGAGAATGAATTTCTCCGTATGCAGGCCATTCACAACCAGTCAGAAATGGACACACTCCAAAAGAAACTAGACCTCTGCCTTGAAGAGAAGGAGACTTTGGAAAGGAATATCAATGATTTGATGACAAAACTTGAAGAAGAGAGATCGGCAAGAGCAATGAAAGAAAAGACACATCAAGTTGAGCTTCCTTCATCCTCAGCTGATGTGCCGATTATGAACTTCAGTGATCAATTGGAACTGAAGACAATGGTCGATGCCATTGGTGCTGCAAGTCAAAGAGAAGCTGAAGCTCATGAGACTGCAATAATCCTGTCCAAAGAAAATGATGAACTGCGTATGAAGCTTAAGGTTTTGATTGAAGATAACAATAAACTCATTGAATTATATGAAGGGGCGACTTCAGAGACTAGTTACAGAAATATTAACAAATCTGAGTGTGCTCATGATGGCACCAAACGCCACAGTAATGGTGGTGGTTTTGTAGAACTTGCCAAAGAAAAGGAGGCTGAGATGAATAAAGTGGTTGAGAATTTGGAGCATCAACTTGCGGATTTGCATGAAGAAAACGAGAAACTAATGGGTTTGTATGAAAGAGCCATGCAAGAGAGGGATGAACTCAAAAGGGTGCTTGCATCTGGTGGACAGGAAAAAGTTACAGCTAAGGATGTGCTTGCTTCTGATGGAGGTGCATCTCCTATGTCTCTGGAGGGAAAGAATTTTATTGGCGATGATGGTCTTTCTGGTTCTGATGGAGGACCAGTCCCAGTGGAGGAATCTGGTTTATGTGGAGCGAATGTGCAAACTGGGTTCAGCCATATATCTGATGAAGTGAAAGCAGATATTGAAGAAAGTGGTGGCTCAAAATCTGTGCTTGATATGGCCGGTCTATGTACCGTTAACACTAAAGGGGATTCAGGAAATGAGTCTGATGTTGGAATTGCGTCTGACATGGAACTGGACACGTCAGATCTCACCACAGTAAAGCTTTCAGAAGATCTCAATTTGGCCAGAATGAATCTGGAAAGAGCAGATGAGCAACTTCTAGATTCTGCAAAAACAGTTGCCGTATTTGGTTGCATGGAGAAATTAATCTTAGAGGTTGGCAAACTCTCAAGAGAAATTGAAGCAACGGAAGATGAAATTCAAGTCAAGCAGCAGCTTTTTGAATCCTATGAACTCCTCTCTGAAAAACTGAAAGAAAACAGCACTCTAATTGACAAAAAGTTGTCAGCTCTCAAGTACTCCCTATCTTGCTTTTCTTCCTCAGTTGTTTACTTTGAGCAGCGAGAGGCTCGGGCAAGAGCAAGGGTAGCAGCTTCGACATCTTATCTGGACCAAAAGAATGGGGAATTGGTCTTCCTACAAACACAAAAGGATGAAATAGCTGCTGAGCAGACGAAAATGCAACAATCTGAGGTCGAACTAAAGAAAAGTCTTGCATGCCTCAAGTCAAAGCTGGATGATGAAAATAAAAAACAAGAGAATGAACAGGTTCTCTTTGCGATAGATAATGTTGAGAAGACAGACCCCTCACAGAAAAATTGGCATTTGGGAGGTAAAGCTACTGAGTTACTGAAGTCCGCGGAAGAGAAAACTAAACTACAGACAGAGATGAAACTATGTCGAGAAAATATTGGAGTCACGAGAAGGGAACTTGAGGATCTGAACAGGAAGTTTGGGAAGGTAGACAGTGAGCTGGTAGCTGTACAAGTGGAGATGCAGAAAGCTGTGAAGTCAGTGGGAGAGATGGAACTTGCACTCGAGAATGCAATGAAGGAGAAAGAAATGCTGTTGGAAGTAAGTGATAACGGAAATACTGAAATTGAAAGCCTGGTTATTGAGTACCAGCAGCATTTGTTTGAATCGGAATTGAAGGAGGCAGAGTCAAAGATTTTAGAGGAAGACTTGCAGACTGAATTACGAAAGTTGGAAAAGCTGCGCAAAAAAAGGGTTTTAACGGCTGAGAAGACAATGCTGTTGTTGGACACGAGGTTTCATCCATGCTTGTTATCAGAAAAGATGGAGGAAGAGCTTCAGAGTGTTAGGAAATATGTTGTAGAGGCAAAGTCATTGCTGCTAGGTGAGGCCAATTCAACTGTCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0002376 GO:0002478 GO:0002495 GO:0002504 GO:0003674 GO:0003676 GO:0003677 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0005815 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005873 GO:0005875 GO:0006810 GO:0006890 GO:0006928 GO:0007017 GO:0007018 GO:0007049 GO:0008150 GO:0008283 GO:0009987 GO:0015630 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019882 GO:0019884 GO:0019886 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0048002 GO:0048193 GO:0051179 GO:0051234 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1388

Amino Acids

155.56

Weight (kDa)

5.17

Isoelectric Point (pI)

44.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	82 - 234	6.6e-21	Microtubule binding
Kinesin	PF00225	88 - 418	2.7e-112	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011008)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44050
fragaria_vesca	FvH4_3g26820 FvH4_3g26820
malus_domestica	MD03G1160900.v1.1 MD11G1176500.v1.1
prunus_persica	Prupe.6G149000_v2.0.a1 Prupe.6G149000_v2.0.a1
pyrus_communis	pycom03g11310 pycom03g11320 pycom11g15070
rosa_chinensis	RchiOBHm_Chr5g0049231
rosa_laevigata	RLG00000034603
rosa_multiflora	Rmu_sc0005358.1_g000003
rosa_roxburghii	Rroxscaffold_1G00032440
rosa_rugosa	Rorug05G0245900
rosa_samantha	Rh5AG323900 Rh5BG334500 Rh5CG359200 Rh5DG346600
rosa_wichuraiana	Rw5G030590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	3998
Acc36I	ACCTGC	1 cut(s)	241
AccB1I	GGYRCC	1 cut(s)	2507
AccI	GTMKAC	1 cut(s)	3744
AccII	CGCG	3 cut(s)	104, 1245, 3641
AccIII	TCCGGA	1 cut(s)	75
AclI	AACGTT	1 cut(s)	1049
AclWI	GGATC	5 cut(s)	979, 1423, 1432, 1459, 3726
AcoI	YGGCCR	7 cut(s)	352, 426, 487, 625, 1596, 2937, 3063
AdeI	CACNNNGTG	1 cut(s)	247
AfaI	GTAC	5 cut(s)	178, 2950, 3299, 3765, 3902
AfeI	AGCGCT	1 cut(s)	283
AfiI	CCNNNNNNNGG	6 cut(s)	45, 564, 1518, 1519, 2080, 2827
AflII	CTTAAG	1 cut(s)	2402
AflIII	ACRYGT	2 cut(s)	1080, 3018
AhlI	ACTAGT	1 cut(s)	2465
AjiI	CACGTC	1 cut(s)	3021
AjnI	CCWGG	6 cut(s)	405, 1411, 1420, 1578, 1787, 3888
AjuI	GAANNNNNNNTTGG	8 cut(s)	101, 133, 798, 830, 2138, 2170, 3392, 3424
AloI	GAACNNNNNNTCC	2 cut(s)	401, 433
Alw21I	GWGCWC	5 cut(s)	83, 793, 992, 1226, 2500
Alw26I	GTCTC	9 cut(s)	135, 879, 1044, 1208, 1697, 2144, 2348, 2456, 2763
AlwI	GGATC	5 cut(s)	979, 1423, 1432, 1459, 3726
AlwNI	CAGNNNCTG	1 cut(s)	974
Ama87I	CYCGRG	4 cut(s)	598, 792, 3351, 3818
Aor13HI	TCCGGA	1 cut(s)	75
Aor51HI	AGCGCT	1 cut(s)	283
AoxI	GGCC	9 cut(s)	352, 426, 487, 625, 1596, 2082, 2937, 3063, 4143
ArsI	GACNNNNNNTTYG	2 cut(s)	1079, 1111
AseI	ATTAAT	1 cut(s)	3146
Asp700I	GAANNNNTTC	2 cut(s)	3549, 4089
AspLEI	GCGC	6 cut(s)	29, 127, 284, 1245, 1247, 3999
AspS9I	GGNCC	8 cut(s)	73, 238, 694, 703, 1442, 1750, 2813, 3391
AsuC2I	CCSGG	1 cut(s)	71
AsuHPI	GGTGA	8 cut(s)	923, 1016, 1087, 1868, 1990, 2025, 3022, 4151
AsuNHI	GCTAGC	1 cut(s)	863
AvaI	CYCGRG	4 cut(s)	598, 792, 3351, 3818
AvaII	GGWCC	8 cut(s)	73, 238, 694, 703, 1442, 1750, 2813, 3391
BalI	TGGCCA	4 cut(s)	354, 489, 1598, 3065
BanI	GGYRCC	1 cut(s)	2507
BanII	GRGCYC	3 cut(s)	992, 1762, 1947
BarI	GAAGNNNNNNTAC	2 cut(s)	1829, 1861
BauI	CACGAG	2 cut(s)	3700, 4045
BbsI	GAAGAC	9 cut(s)	225, 512, 741, 2037, 2306, 3406, 3584, 3966, 4031
Bbv12I	GWGCWC	5 cut(s)	83, 793, 992, 1226, 2500
BbvCI	CCTCAGC	1 cut(s)	2254
BceAI	ACGGC	2 cut(s)	3107, 4032
BciT130I	CCWGG	6 cut(s)	407, 1413, 1422, 1580, 1789, 3890
BciVI	GTATCC	1 cut(s)	1351
BclI	TGATCA	1 cut(s)	2284
BcnI	CCSGG	1 cut(s)	71
BcoDI	GTCTC	9 cut(s)	135, 879, 1044, 1208, 1697, 2144, 2348, 2456, 2763
BcuI	ACTAGT	1 cut(s)	2465
BfaI	CTAG	8 cut(s)	576, 864, 1197, 1473, 2126, 2466, 3101, 4136
BfmI	CTRYAG	1 cut(s)	3659
BfoI	RGCGCY	3 cut(s)	30, 128, 285
BfrI	CTTAAG	1 cut(s)	2402
BfuAI	ACCTGC	1 cut(s)	241
BfuI	GTATCC	1 cut(s)	1351
BglII	AGATCT	3 cut(s)	1692, 3025, 3052
BlpI	GCTNAGC	2 cut(s)	275, 3447
BmcAI	AGTACT	1 cut(s)	3299
Bme1390I	CCNGG	7 cut(s)	71, 407, 1413, 1422, 1580, 1789, 3890
Bme18I	GGWCC	8 cut(s)	73, 238, 694, 703, 1442, 1750, 2813, 3391
BmeT110I	CYCGRG	4 cut(s)	598, 792, 3351, 3818
BmgBI	CACGTC	1 cut(s)	3021
BmgT120I	GGNCC	8 cut(s)	73, 238, 694, 703, 1442, 1750, 2813, 3391
BmiI	GGNNCC	3 cut(s)	50, 705, 2509
BmrFI	CCNGG	7 cut(s)	71, 407, 1413, 1422, 1580, 1789, 3890
BmrI	ACTGGG	3 cut(s)	154, 2816, 2871
BmsI	GCATC	9 cut(s)	144, 903, 1295, 2039, 2302, 2605, 2699, 2756, 3765
BmtI	GCTAGC	1 cut(s)	867
BmuI	ACTGGG	3 cut(s)	154, 2816, 2871
BoxI	GACNNNNGTC	1 cut(s)	2306
BpiI	GAAGAC	9 cut(s)	225, 512, 741, 2037, 2306, 3406, 3584, 3966, 4031
BplI	GAGNNNNNCTC	4 cut(s)	104, 136, 1471, 1503
BpmI	CTGGAG	4 cut(s)	1014, 1311, 1404, 2783
Bpu10I	CCTNAGC	2 cut(s)	2254, 2718
Bpu1102I	GCTNAGC	2 cut(s)	275, 3447
BpuEI	CTTGAG	5 cut(s)	1313, 3154, 3278, 3488, 3734
BpuMI	CCSGG	1 cut(s)	71
BsaBI	GATNNNNATC	1 cut(s)	1965
BsaI	GGTCTC	1 cut(s)	879
BsaJI	CCNNGG	3 cut(s)	919, 2036, 3639
BsaWI	WCCGGW	1 cut(s)	75
Bsc4I	CCNNNNNNNGG	6 cut(s)	45, 564, 1518, 1519, 2080, 2827
Bse118I	RCCGGY	1 cut(s)	2939
Bse3DI	GCAATG	6 cut(s)	1299, 1553, 1803, 2220, 3834, 4127
Bse8I	GATNNNNATC	1 cut(s)	1965
BseAI	TCCGGA	1 cut(s)	75
BseBI	CCWGG	6 cut(s)	407, 1413, 1422, 1580, 1789, 3890
BseDI	CCNNGG	3 cut(s)	919, 2036, 3639
BseGI	GGATG	9 cut(s)	355, 573, 1618, 2249, 2674, 2728, 3439, 3523, 4054
BseJI	GATNNNNATC	1 cut(s)	1965
BseLI	CCNNNNNNNGG	6 cut(s)	45, 564, 1518, 1519, 2080, 2827
BseMI	GCAATG	6 cut(s)	1299, 1553, 1803, 2220, 3834, 4127
BsePI	GCGCGC	1 cut(s)	1243
BseRI	GAGGAG	3 cut(s)	1742, 2072, 3227
BsgI	GTGCAG	2 cut(s)	269, 843
Bsh1236I	CGCG	3 cut(s)	104, 1245, 3641
BshFI	GGCC	9 cut(s)	354, 428, 489, 627, 1598, 2084, 2939, 3065, 4145
BshNI	GGYRCC	1 cut(s)	2507
BsiHKAI	GWGCWC	5 cut(s)	83, 793, 992, 1226, 2500
BsiHKCI	CYCGRG	4 cut(s)	598, 792, 3351, 3818
BsiSI	CCGG	4 cut(s)	71, 76, 429, 2940
BslFI	GGGAC	3 cut(s)	161, 1068, 2804
BslI	CCNNNNNNNGG	6 cut(s)	45, 564, 1518, 1519, 2080, 2827
BsmAI	GTCTC	9 cut(s)	135, 879, 1044, 1208, 1697, 2144, 2348, 2456, 2763
BsmFI	GGGAC	3 cut(s)	161, 1068, 2804
BsmI	GAATGC	2 cut(s)	920, 3829
BsnI	GGCC	9 cut(s)	354, 428, 489, 627, 1598, 2084, 2939, 3065, 4145
Bso31I	GGTCTC	1 cut(s)	879
BsoBI	CYCGRG	4 cut(s)	598, 792, 3351, 3818
Bsp1286I	GDGCHC	7 cut(s)	83, 793, 992, 1226, 1762, 1947, 2500
Bsp13I	TCCGGA	1 cut(s)	75
Bsp1407I	TGTACA	1 cut(s)	3763
Bsp1720I	GCTNAGC	2 cut(s)	275, 3447
BspANI	GGCC	9 cut(s)	354, 428, 489, 627, 1598, 2084, 2939, 3065, 4145
BspEI	TCCGGA	1 cut(s)	75
BspFNI	CGCG	3 cut(s)	104, 1245, 3641
BspHI	TCATGA	3 cut(s)	214, 2350, 2500
BspLI	GGNNCC	3 cut(s)	50, 705, 2509
BspMI	ACCTGC	1 cut(s)	241
BspOI	GCTAGC	1 cut(s)	867
BspPI	GGATC	5 cut(s)	979, 1423, 1432, 1459, 3726
BspQI	GCTCTTC	1 cut(s)	4080
BspT107I	GGYRCC	1 cut(s)	2507
BspTI	CTTAAG	1 cut(s)	2402
BspTNI	GGTCTC	1 cut(s)	879
BsrDI	GCAATG	6 cut(s)	1299, 1553, 1803, 2220, 3834, 4127
BsrFI	RCCGGY	1 cut(s)	2939
BsrGI	TGTACA	1 cut(s)	3763
BssAI	RCCGGY	1 cut(s)	2939
BssECI	CCNNGG	3 cut(s)	919, 2036, 3639
BssHII	GCGCGC	1 cut(s)	1243
BssSI	CACGAG	2 cut(s)	3700, 4045
BssT1I	CCWWGG	2 cut(s)	919, 2036
Bst2BI	CACGAG	2 cut(s)	3700, 4045
Bst2UI	CCWGG	6 cut(s)	407, 1413, 1422, 1580, 1789, 3890
Bst6I	CTCTTC	5 cut(s)	67, 2136, 2190, 3639, 4080
BstAFI	CTTAAG	1 cut(s)	2402
BstAUI	TGTACA	1 cut(s)	3763
BstC8I	GCNNGC	9 cut(s)	25, 625, 865, 1245, 1596, 2082, 2688, 2730, 3499
BstDSI	CCRYGG	1 cut(s)	3639
BstF5I	GGATG	9 cut(s)	355, 573, 1618, 2249, 2674, 2728, 3439, 3523, 4054
BstFNI	CGCG	3 cut(s)	104, 1245, 3641
BstH2I	RGCGCY	3 cut(s)	30, 128, 285
BstHHI	GCGC	6 cut(s)	29, 127, 284, 1245, 1247, 3999
BstMAI	GTCTC	9 cut(s)	135, 879, 1044, 1208, 1697, 2144, 2348, 2456, 2763
BstMWI	GCNNNNNNNGC	9 cut(s)	179, 459, 468, 1197, 1244, 2321, 2398, 2514, 3368
BstNI	CCWGG	6 cut(s)	407, 1413, 1422, 1580, 1789, 3890
BstNSI	RCATGY	4 cut(s)	478, 894, 1084, 3501
BstPAI	GACNNNNGTC	1 cut(s)	2306
BstSCI	CCNGG	7 cut(s)	69, 405, 1411, 1420, 1578, 1787, 3888
BstSFI	CTRYAG	1 cut(s)	3659
BstUI	CGCG	3 cut(s)	104, 1245, 3641
BstV2I	GAAGAC	9 cut(s)	225, 512, 741, 2037, 2306, 3406, 3584, 3966, 4031
BstX2I	RGATCY	7 cut(s)	971, 1415, 1424, 1692, 3025, 3052, 3718
BstXI	CCANNNNNNTGG	2 cut(s)	2525, 3401
BstYI	RGATCY	7 cut(s)	971, 1415, 1424, 1692, 3025, 3052, 3718
BsuI	GTATCC	1 cut(s)	1351
BsuRI	GGCC	9 cut(s)	354, 428, 489, 627, 1598, 2084, 2939, 3065, 4145
BtgI	CCRYGG	1 cut(s)	3639
BtgZI	GCGATG	1 cut(s)	2799
BtrI	CACGTC	1 cut(s)	3021
BtsCI	GGATG	9 cut(s)	355, 573, 1618, 2249, 2674, 2728, 3439, 3523, 4054
BtsIMutI	CAGTG	7 cut(s)	300, 559, 1217, 2287, 2829, 3754, 3801
BveI	ACCTGC	1 cut(s)	241
Cac8I	GCNNGC	9 cut(s)	25, 625, 865, 1245, 1596, 2082, 2688, 2730, 3499
CaiI	CAGNNNCTG	1 cut(s)	974
CciI	TCATGA	3 cut(s)	214, 2350, 2500
CfoI	GCGC	6 cut(s)	29, 127, 284, 1245, 1247, 3999
Cfr10I	RCCGGY	1 cut(s)	2939
Cfr13I	GGNCC	8 cut(s)	73, 238, 694, 703, 1442, 1750, 2813, 3391
Cfr42I	CCGCGG	1 cut(s)	3642
CpoI	CGGWCCG	1 cut(s)	73
CseI	GACGC	4 cut(s)	93, 998, 1292, 2988
CsiI	ACCWGGT	1 cut(s)	1787
Csp6I	GTAC	5 cut(s)	177, 2949, 3298, 3764, 3901
CspI	CGGWCCG	1 cut(s)	73
CviQI	GTAC	5 cut(s)	177, 2949, 3298, 3764, 3901
DraIII	CACNNNGTG	1 cut(s)	247
EaeI	YGGCCR	7 cut(s)	352, 426, 487, 625, 1596, 2937, 3063
Eam1104I	CTCTTC	5 cut(s)	67, 2136, 2190, 3639, 4080
EarI	CTCTTC	5 cut(s)	67, 2136, 2190, 3639, 4080
Ecl136II	GAGCTC	1 cut(s)	990
Eco130I	CCWWGG	2 cut(s)	919, 2036
Eco24I	GRGCYC	3 cut(s)	992, 1762, 1947
Eco31I	GGTCTC	1 cut(s)	879
Eco47I	GGWCC	8 cut(s)	73, 238, 694, 703, 1442, 1750, 2813, 3391
Eco47III	AGCGCT	1 cut(s)	283
Eco53kI	GAGCTC	1 cut(s)	990
Eco88I	CYCGRG	4 cut(s)	598, 792, 3351, 3818
EcoICRI	GAGCTC	1 cut(s)	990
EcoO109I	RGGNCCY	2 cut(s)	1442, 1750
EcoRI	GAATTC	2 cut(s)	603, 1159
EcoRII	CCWGG	6 cut(s)	405, 1411, 1420, 1578, 1787, 3888
EcoT14I	CCWWGG	2 cut(s)	919, 2036
EcoT22I	ATGCAT	2 cut(s)	896, 1935
EcoT38I	GRGCYC	3 cut(s)	992, 1762, 1947
ErhI	CCWWGG	2 cut(s)	919, 2036
FalI	AAGNNNNNCTT	6 cut(s)	26, 58, 1497, 1529, 2226, 2258
FaqI	GGGAC	3 cut(s)	161, 1068, 2804
FauI	CCCGC	2 cut(s)	47, 557
FbaI	TGATCA	1 cut(s)	2284
FblI	GTMKAC	1 cut(s)	3744
FokI	GGATG	9 cut(s)	342, 580, 1625, 2236, 2681, 2735, 3446, 3530, 4041
FriOI	GRGCYC	3 cut(s)	992, 1762, 1947
FspBI	CTAG	8 cut(s)	576, 864, 1197, 1473, 2126, 2466, 3101, 4136
FspI	TGCGCA	1 cut(s)	3998
GlaI	GCGC	6 cut(s)	28, 126, 283, 1244, 1246, 3998
GsuI	CTGGAG	4 cut(s)	1014, 1311, 1404, 2783
HaeII	RGCGCY	3 cut(s)	30, 128, 285
HaeIII	GGCC	9 cut(s)	354, 428, 489, 627, 1598, 2084, 2939, 3065, 4145
HapII	CCGG	4 cut(s)	71, 76, 429, 2940
HgaI	GACGC	4 cut(s)	93, 998, 1292, 2988
HhaI	GCGC	6 cut(s)	29, 127, 284, 1245, 1247, 3999
Hin6I	GCGC	6 cut(s)	27, 125, 282, 1243, 1245, 3997
HinP1I	GCGC	6 cut(s)	27, 125, 282, 1243, 1245, 3997
HincII	GTYRAC	2 cut(s)	961, 2956
HindII	GTYRAC	2 cut(s)	961, 2956
HindIII	AAGCTT	4 cut(s)	202, 1124, 2399, 3041
HpaI	GTTAAC	1 cut(s)	2956
HpaII	CCGG	4 cut(s)	71, 76, 429, 2940
HphI	GGTGA	8 cut(s)	923, 1016, 1087, 1868, 1990, 2025, 3022, 4151
Hpy166II	GTNNAC	9 cut(s)	372, 445, 961, 1078, 1859, 2699, 2956, 3331, 3745
Hpy8I	GTNNAC	9 cut(s)	372, 445, 961, 1078, 1859, 2699, 2956, 3331, 3745
Hpy99I	CGWCG	2 cut(s)	155, 167
HpyCH4IV	ACGT	4 cut(s)	246, 1049, 1562, 3020
HpyF10VI	GCNNNNNNNGC	9 cut(s)	179, 459, 468, 1197, 1244, 2321, 2398, 2514, 3368
HpySE526I	ACGT	4 cut(s)	246, 1049, 1562, 3020
HspAI	GCGC	6 cut(s)	27, 125, 282, 1243, 1245, 3997
Kpn2I	TCCGGA	1 cut(s)	75
Ksp22I	TGATCA	1 cut(s)	2284
KspAI	GTTAAC	1 cut(s)	2956
KspI	CCGCGG	1 cut(s)	3642
LguI	GCTCTTC	1 cut(s)	4080
LmnI	GCTCC	6 cut(s)	78, 132, 409, 995, 2593, 2846
LweI	GCATC	9 cut(s)	144, 903, 1295, 2039, 2302, 2605, 2699, 2756, 3765
MabI	ACCWGGT	1 cut(s)	1787
MaeI	CTAG	8 cut(s)	576, 864, 1197, 1473, 2126, 2466, 3101, 4136
MaeII	ACGT	4 cut(s)	246, 1049, 1562, 3020
MfeI	CAATTG	4 cut(s)	413, 559, 1269, 2288
MflI	RGATCY	7 cut(s)	971, 1415, 1424, 1692, 3025, 3052, 3718
MhlI	GDGCHC	7 cut(s)	83, 793, 992, 1226, 1762, 1947, 2500
MlsI	TGGCCA	4 cut(s)	354, 489, 1598, 3065
MluNI	TGGCCA	4 cut(s)	354, 489, 1598, 3065
MlyI	GAGTC	5 cut(s)	371, 1946, 2990, 3705, 3950
MmeI	TCCRAC	7 cut(s)	829, 1191, 1650, 2971, 3831, 3966, 4020
Mox20I	TGGCCA	4 cut(s)	354, 489, 1598, 3065
Mph1103I	ATGCAT	2 cut(s)	896, 1935
MroI	TCCGGA	1 cut(s)	75
MroXI	GAANNNNTTC	2 cut(s)	3549, 4089
MscI	TGGCCA	4 cut(s)	354, 489, 1598, 3065
MslI	CAYNNNNRTG	3 cut(s)	1304, 1310, 2523
Msp20I	TGGCCA	4 cut(s)	354, 489, 1598, 3065
MspA1I	CMGCKG	4 cut(s)	1594, 1700, 2258, 3641
MspCI	CTTAAG	1 cut(s)	2402
MspI	CCGG	4 cut(s)	71, 76, 429, 2940
MspR9I	CCNGG	7 cut(s)	71, 407, 1413, 1422, 1580, 1789, 3890
MunI	CAATTG	4 cut(s)	413, 559, 1269, 2288
Mva1269I	GAATGC	2 cut(s)	920, 3829
MvaI	CCWGG	6 cut(s)	407, 1413, 1422, 1580, 1789, 3890
MvnI	CGCG	3 cut(s)	104, 1245, 3641
MwoI	GCNNNNNNNGC	9 cut(s)	179, 459, 468, 1197, 1244, 2321, 2398, 2514, 3368
NciI	CCSGG	1 cut(s)	71
NheI	GCTAGC	1 cut(s)	863
NlaIV	GGNNCC	3 cut(s)	50, 705, 2509
NmeAIII	GCCGAG	3 cut(s)	101, 653, 925
NmuCI	GTSAC	5 cut(s)	363, 781, 1095, 1783, 3697
NsbI	TGCGCA	1 cut(s)	3998
NsiI	ATGCAT	2 cut(s)	896, 1935
NspI	RCATGY	4 cut(s)	478, 894, 1084, 3501
PaeI	GCATGC	1 cut(s)	3501
PaeR7I	CTCGAG	3 cut(s)	598, 792, 3818
PagI	TCATGA	3 cut(s)	214, 2350, 2500
PauI	GCGCGC	1 cut(s)	1243
PciI	ACATGT	1 cut(s)	1080
PciSI	GCTCTTC	1 cut(s)	4080
PcsI	WCGNNNNNNNCGW	2 cut(s)	51, 159
PctI	GAATGC	2 cut(s)	920, 3829
PdmI	GAANNNNTTC	2 cut(s)	3549, 4089
PflFI	GACNNNGTC	1 cut(s)	71
PfoI	TCCNGGA	2 cut(s)	1411, 1420
PleI	GAGTC	5 cut(s)	370, 1945, 2989, 3704, 3949
PpsI	GAGTC	5 cut(s)	370, 1945, 2989, 3704, 3949
PpuMI	RGGWCCY	2 cut(s)	1442, 1750
PscI	ACATGT	1 cut(s)	1080
PshAI	GACNNNNGTC	1 cut(s)	2306
PshBI	ATTAAT	1 cut(s)	3146
Psp124BI	GAGCTC	1 cut(s)	992
Psp1406I	AACGTT	1 cut(s)	1049
Psp5II	RGGWCCY	2 cut(s)	1442, 1750
Psp6I	CCWGG	6 cut(s)	405, 1411, 1420, 1578, 1787, 3888
PspGI	CCWGG	6 cut(s)	405, 1411, 1420, 1578, 1787, 3888
PspN4I	GGNNCC	3 cut(s)	50, 705, 2509
PspPI	GGNCC	8 cut(s)	73, 238, 694, 703, 1442, 1750, 2813, 3391
PspPPI	RGGWCCY	2 cut(s)	1442, 1750
PstNI	CAGNNNCTG	1 cut(s)	974
PsuI	RGATCY	7 cut(s)	971, 1415, 1424, 1692, 3025, 3052, 3718
PsyI	GACNNNGTC	1 cut(s)	71
PteI	GCGCGC	1 cut(s)	1243
PvuII	CAGCTG	3 cut(s)	1594, 1700, 2258
RsaI	GTAC	5 cut(s)	178, 2950, 3299, 3765, 3902
RsaNI	GTAC	5 cut(s)	177, 2949, 3298, 3764, 3901
RseI	CAYNNNNRTG	3 cut(s)	1304, 1310, 2523
Rsr2I	CGGWCCG	1 cut(s)	73
RsrII	CGGWCCG	1 cut(s)	73
SacI	GAGCTC	1 cut(s)	992
SacII	CCGCGG	1 cut(s)	3642
SapI	GCTCTTC	1 cut(s)	4080
Sau96I	GGNCC	8 cut(s)	73, 238, 694, 703, 1442, 1750, 2813, 3391
ScaI	AGTACT	1 cut(s)	3299
SchI	GAGTC	5 cut(s)	371, 1946, 2990, 3705, 3950
ScrFI	CCNGG	7 cut(s)	71, 407, 1413, 1422, 1580, 1789, 3890
SduI	GDGCHC	7 cut(s)	83, 793, 992, 1226, 1762, 1947, 2500
SexAI	ACCWGGT	1 cut(s)	1787
SfaNI	GCATC	9 cut(s)	144, 903, 1295, 2039, 2302, 2605, 2699, 2756, 3765
SfcI	CTRYAG	1 cut(s)	3659
Sfr274I	CTCGAG	3 cut(s)	598, 792, 3818
Sfr303I	CCGCGG	1 cut(s)	3642
SgrBI	CCGCGG	1 cut(s)	3642
SinI	GGWCC	8 cut(s)	73, 238, 694, 703, 1442, 1750, 2813, 3391
SlaI	CTCGAG	3 cut(s)	598, 792, 3818
SmiMI	CAYNNNNRTG	3 cut(s)	1304, 1310, 2523
SmlI	CTYRAG	9 cut(s)	598, 792, 1328, 2402, 3169, 3293, 3503, 3713, 3818
SmoI	CTYRAG	9 cut(s)	598, 792, 1328, 2402, 3169, 3293, 3503, 3713, 3818
SpeI	ACTAGT	1 cut(s)	2465
SphI	GCATGC	1 cut(s)	3501
SspI	AATATT	4 cut(s)	1030, 1186, 2479, 3691
SspMI	CTAG	8 cut(s)	576, 864, 1197, 1473, 2126, 2466, 3101, 4136
SstI	GAGCTC	1 cut(s)	992
StyD4I	CCNGG	7 cut(s)	69, 405, 1411, 1420, 1578, 1787, 3888
StyI	CCWWGG	2 cut(s)	919, 2036
TaiI	ACGT	4 cut(s)	249, 1052, 1565, 3023
TaqII	GACCGA	1 cut(s)	861
TatI	WGTACW	2 cut(s)	3297, 3763
TauI	GCSGC	1 cut(s)	1813
TscAI	CASTG	7 cut(s)	300, 559, 1224, 2287, 2829, 3754, 3801
TseFI	GTSAC	5 cut(s)	363, 781, 1095, 1783, 3697
Tsp45I	GTSAC	5 cut(s)	363, 781, 1095, 1783, 3697
TspGWI	ACGGA	5 cut(s)	145, 180, 2063, 3203, 3882
TspRI	CASTG	7 cut(s)	300, 559, 1224, 2287, 2829, 3754, 3801
Tth111I	GACNNNGTC	1 cut(s)	71
Vha464I	CTTAAG	1 cut(s)	2402
VpaK11BI	GGWCC	8 cut(s)	73, 238, 694, 703, 1442, 1750, 2813, 3391
VspI	ATTAAT	1 cut(s)	3146
XbaI	TCTAGA	2 cut(s)	1472, 3100
XceI	RCATGY	4 cut(s)	478, 894, 1084, 3501
XcmI	CCANNNNNNNNNTGG	5 cut(s)	497, 518, 1795, 2525, 3073
XhoI	CTCGAG	3 cut(s)	598, 792, 3818
XmiI	GTMKAC	1 cut(s)	3744
XmnI	GAANNNNTTC	2 cut(s)	3549, 4089
XspI	CTAG	8 cut(s)	576, 864, 1197, 1473, 2126, 2466, 3101, 4136
ZrmI	AGTACT	1 cut(s)	3299
Zsp2I	ATGCAT	2 cut(s)	896, 1935

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.