Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0009152

Physical Location & Seq

Genomic Coordinates Reverse (-)

30610063 .. 30614708

Gene Structure 4646 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1209400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGGCTGTCGCGGATGCTCAAAATCCTCGTGTTGGAGAAACCACTTGTGGTTCTTTGCTGCAGAAACTGCAGGAAATTTGGAATGAAGTTGGCGAGAGTGATGAAGAACGAGACAAAATGCTTCTTCAGTTAGAGCAAGAGTGCCTTGATGTATACAAGAGGAAGGTCGAGCTGGCAGCTAAGTCAAGGGCACAGCTTCTTCAGGCATTGTCTGATTCCAGACTTGAACTTTCCAGTCTTCTATCAGCTCTTGGAGAAAAAAGTTTTACCGGAGTTCCTGAGAAGACTTCAGGGACAATCAAGGAACAGCTTGGGGCTATAGCGCCACTACTGGAACAGCTGTGGGAACAGAAAGAGGAGAGAGTGAAGGAGTTTACTGATGTTCAGTCTCAAATTCAGAAGATTTGTGGAGAAATTGCTGGGAACTCGAATCTCAGCGACAGTCCTGCGGTTGATGAGTCTGATCTATCCCTGAAGAAATTAGATGAATTTCAATCCCAACTTCATGATCTTCAAAAGGAAAAGAGTGAAAGATTGCACAAGGTGCTTGAATTCGTGAGCACAGTGCACGATCTTTGTGCTGTACTTGGACTGGACTTCTTGAGTACTGTTACAGAGGTTCATCCTAGCTTAAATGACTCAACTGGTGTGCGTACCAAAAGCATTAGCAATGATACTCTATCTAGGCTGGCAAGGACTGTCTTAGCACTAAAAGAAGATAAGGAACAGAGGCTGCATAAGCTTCAAGAGTCAGCAACTCAGCTCATTGATCTGTGGAATCTGATGGATACCCCTGACGAGGAAAGGAGATTATTTGAACATGTAACCTGTAACATATCAGCTTCAGTAGATGAAGTGACTGTTCCTGGGTCTCTTGCGCTGGATCTGATTGAACAGACTGAGGTTGAAGTTGACCGGCTCGATCAGCTGAAGTCAAGCAGGATGAAAGAAATTGCGTTCAAGAAGCAAGCAGAGCTAGAGGAGATATTTGCTCGAGCTCATGTAGAAATAGATACAGAAGCTGCCAGAGAAAAGATTATGGAACTCATTGATTCGGGGAATGTCGAACCTACTGAATTACTGGCTGACATGGATGATCAGATAGCGAAAGCGATGGATGAAGCTCTAAGCAGAAAGGATATACTGGACAAGGTTGAGAAATGGATGTCAGCCTGTGAGGAAGAGAGTTGGCTTGAGGACTACAATCGCGATGAAAACAGGTATAATGCGAGTAGAGGTGCGCACCTAAACCTCAAGCGTGCAGAGAAAGCACGAATTCTGGTTAACAAAATTCCAGCTCTTGTAGACACGTTGGTAGCCAAAACACGTGGGTGGGAGGAAGAGCGTGGCATATCATTTACTTATGATGGTGTTCCTCTCCTTGCTATGCTGGATGAGTATGCCATGCTGAGGCAAGAAAGAGAAGAAGAGAAACGGAGGATGAGGGATCAGAAGAAGTACCAAGAGCTACAACATACAGAACAAGAAACCATATTTGGTTCAAAGCCTAGCCCTGCTCGACCAGTTGGCACAAAGAAGGTAGTAGGTCCTCGTGCAAATGGTGGCCCCAATGGAACTCCTAGCAGACGGCTTTCACTTAATGCTCATCAAAATGGAAGCAGGTCTGTAGTGAAAGATGGGAAGAGAGATCTTAGGCCCGTCGCTCCTATGAACTACGTTGAAATGGCAAAAGAGGATGCTGCCTCCCACATTTCTGGCACTGACACGGTTCCTGCTTCACCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000922 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007275 GO:0008150 GO:0008283 GO:0009524 GO:0009574 GO:0009791 GO:0009987 GO:0015630 GO:0016043 GO:0022402 GO:0022622 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032501 GO:0032502 GO:0032506 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043622 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0046983 GO:0048364 GO:0048528 GO:0048731 GO:0048856 GO:0051301 GO:0055028 GO:0061640 GO:0071840 GO:0071944 GO:0072686 GO:0090696 GO:0097435 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MAP65_ASE1

Protein Analysis

582

Amino Acids

65.38

Weight (kDa)

5.02

Isoelectric Point (pI)

50.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MAP65_ASE1	PF03999	18 - 505	1.2e-82	Microtubule associated protein (MAP65/ASE1 family)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009152)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55230 AT5G55230 AT5G55230
fragaria_vesca	FvH4_3g23560
malus_domestica	MD03G1193200.v1.1 MD11G1209400.v1.1
prunus_persica	Prupe.4G223500_v2.0.a1 Prupe.4G223500_v2.0.a1
pyrus_communis	pycom03g14580 pycom11g18490
rosa_chinensis	RchiOBHm_Chr5g0041551
rosa_laevigata	RLG00000034078
rosa_multiflora	Rmu_sc0008429.1_g000003
rosa_roxburghii	Rroxscaffold_1G00038960
rosa_rugosa	Rorug05G0193400 Rorug05G0193400 Rorug05G0193500
rosa_samantha	Rh5AG279200 Rh5BG283500 Rh5CG316400 Rh5DG293200
rosa_wichuraiana	Rw5G026290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1242
Acc36I	ACCTGC	1 cut(s)	1611
AccI	GTMKAC	2 cut(s)	153, 1305
AccII	CGCG	2 cut(s)	11, 1209
AciI	CCGC	2 cut(s)	11, 449
AclWI	GGATC	2 cut(s)	891, 1455
AcsI	RAATTY	6 cut(s)	75, 393, 488, 551, 1275, 1290
AcuI	CTGAAG	6 cut(s)	110, 185, 273, 494, 828, 950
AcvI	CACGTG	1 cut(s)	1328
AdeI	CACNNNGTG	1 cut(s)	544
AfaI	GTAC	4 cut(s)	585, 607, 655, 1460
AfiI	CCNNNNNNNGG	3 cut(s)	32, 799, 1410
AflIII	ACRYGT	3 cut(s)	820, 1308, 1325
AjnI	CCWGG	1 cut(s)	865
AjuI	GAANNNNNNNTTGG	2 cut(s)	1479, 1511
AleI	CACNNNNGTG	1 cut(s)	1330
Alw21I	GWGCWC	3 cut(s)	563, 570, 1000
Alw26I	GTCTC	3 cut(s)	105, 393, 876
Alw44I	GTGCAC	1 cut(s)	566
AlwI	GGATC	2 cut(s)	891, 1455
AlwNI	CAGNNNCTG	3 cut(s)	67, 733, 1022
Ama87I	CYCGRG	1 cut(s)	993
AoxI	GGCC	2 cut(s)	1564, 1655
ApaLI	GTGCAC	1 cut(s)	566
ApeKI	GCWGC	5 cut(s)	58, 176, 733, 1022, 1700
ApoI	RAATTY	6 cut(s)	75, 393, 488, 551, 1275, 1290
AspLEI	GCGC	3 cut(s)	325, 880, 1243
AspS9I	GGNCC	3 cut(s)	1547, 1565, 1656
AsuHPI	GGTGA	1 cut(s)	1731
AvaI	CYCGRG	1 cut(s)	993
AvaII	GGWCC	1 cut(s)	1547
BaeGI	GKGCMC	2 cut(s)	193, 570
BanII	GRGCYC	1 cut(s)	1000
BauI	CACGAG	2 cut(s)	27, 1551
BbrPI	CACGTG	1 cut(s)	1328
BbsI	GAAGAC	2 cut(s)	230, 290
Bbv12I	GWGCWC	3 cut(s)	563, 570, 1000
BbvCI	CCTCAGC	1 cut(s)	1409
BbvI	GCAGC	5 cut(s)	45, 188, 720, 1009, 1687
BccI	CCATC	4 cut(s)	778, 1108, 1361, 1631
BceAI	ACGGC	1 cut(s)	1604
BcgI	CGANNNNNNTGC	4 cut(s)	428, 462, 560, 594
BciT130I	CCWGG	1 cut(s)	867
BciVI	GTATCC	1 cut(s)	781
BclI	TGATCA	1 cut(s)	1096
BcoDI	GTCTC	3 cut(s)	105, 393, 876
BfaI	CTAG	5 cut(s)	627, 684, 977, 1509, 1581
BfmI	CTRYAG	4 cut(s)	59, 68, 318, 1626
BfoI	RGCGCY	1 cut(s)	326
BfuAI	ACCTGC	1 cut(s)	1611
BfuI	GTATCC	1 cut(s)	781
BglII	AGATCT	1 cut(s)	1648
BisI	GCNGC	5 cut(s)	59, 177, 734, 1023, 1701
BlsI	GCNGC	5 cut(s)	60, 178, 735, 1024, 1702
BmcAI	AGTACT	1 cut(s)	607
Bme1390I	CCNGG	1 cut(s)	867
Bme18I	GGWCC	1 cut(s)	1547
BmeT110I	CYCGRG	1 cut(s)	993
BmgT120I	GGNCC	3 cut(s)	1547, 1565, 1656
BmiI	GGNNCC	2 cut(s)	1567, 1731
BmrFI	CCNGG	1 cut(s)	867
BmsI	GCATC	2 cut(s)	4, 1687
BpiI	GAAGAC	2 cut(s)	230, 290
Bpu10I	CCTNAGC	1 cut(s)	1409
BpuEI	CTTGAG	3 cut(s)	622, 1214, 1238
BsaAI	YACGTR	1 cut(s)	1328
BsaI	GGTCTC	1 cut(s)	876
BsaJI	CCNNGG	1 cut(s)	866
BsaWI	WCCGGW	1 cut(s)	269
Bsc4I	CCNNNNNNNGG	3 cut(s)	32, 799, 1410
Bse118I	RCCGGY	1 cut(s)	915
Bse1I	ACTGG	7 cut(s)	234, 336, 597, 649, 1086, 1149, 1523
Bse3DI	GCAATG	1 cut(s)	676
BseBI	CCWGG	1 cut(s)	867
BseDI	CCNNGG	1 cut(s)	866
BseGI	GGATG	9 cut(s)	19, 622, 948, 1099, 1123, 1170, 1399, 1446, 1702
BseLI	CCNNNNNNNGG	3 cut(s)	32, 799, 1410
BseMI	GCAATG	1 cut(s)	676
BseMII	CTCAG	5 cut(s)	270, 448, 773, 891, 1400
BseNI	ACTGG	7 cut(s)	234, 336, 597, 649, 1086, 1149, 1523
BseRI	GAGGAG	2 cut(s)	371, 995
BseSI	GKGCMC	2 cut(s)	193, 570
BseXI	GCAGC	5 cut(s)	45, 188, 720, 1009, 1687
BseYI	CCCAGC	1 cut(s)	419
BsgI	GTGCAG	1 cut(s)	1281
Bsh1236I	CGCG	2 cut(s)	11, 1209
BshFI	GGCC	2 cut(s)	1566, 1657
BsiHKAI	GWGCWC	3 cut(s)	563, 570, 1000
BsiHKCI	CYCGRG	1 cut(s)	993
BsiSI	CCGG	2 cut(s)	270, 916
BslFI	GGGAC	1 cut(s)	307
BslI	CCNNNNNNNGG	3 cut(s)	32, 799, 1410
BsmAI	GTCTC	3 cut(s)	105, 393, 876
BsmFI	GGGAC	1 cut(s)	307
BsnI	GGCC	2 cut(s)	1566, 1657
Bso31I	GGTCTC	1 cut(s)	876
BsoBI	CYCGRG	1 cut(s)	993
Bsp1286I	GDGCHC	4 cut(s)	193, 563, 570, 1000
Bsp143I	GATC	9 cut(s)	463, 508, 571, 769, 883, 922, 1096, 1447, 1648
Bsp68I	TCGCGA	1 cut(s)	1209
BspACI	CCGC	2 cut(s)	11, 449
BspANI	GGCC	2 cut(s)	1566, 1657
BspCNI	CTCAG	5 cut(s)	271, 447, 772, 892, 1401
BspFNI	CGCG	2 cut(s)	11, 1209
BspHI	TCATGA	1 cut(s)	505
BspLI	GGNNCC	2 cut(s)	1567, 1731
BspMAI	CTGCAG	2 cut(s)	63, 72
BspMI	ACCTGC	1 cut(s)	1611
BspPI	GGATC	2 cut(s)	891, 1455
BspQI	GCTCTTC	1 cut(s)	1335
BspTNI	GGTCTC	1 cut(s)	876
BsrDI	GCAATG	1 cut(s)	676
BsrFI	RCCGGY	1 cut(s)	915
BsrI	ACTGG	7 cut(s)	234, 336, 597, 649, 1086, 1149, 1523
BssAI	RCCGGY	1 cut(s)	915
BssECI	CCNNGG	1 cut(s)	866
BssMI	GATC	9 cut(s)	463, 508, 571, 769, 883, 922, 1096, 1447, 1648
BssNAI	GTATAC	1 cut(s)	154
BssSI	CACGAG	2 cut(s)	27, 1551
Bst1107I	GTATAC	1 cut(s)	154
Bst2BI	CACGAG	2 cut(s)	27, 1551
Bst2UI	CCWGG	1 cut(s)	867
Bst4CI	ACNGT	6 cut(s)	443, 565, 610, 700, 862, 1729
Bst6I	CTCTTC	4 cut(s)	1176, 1335, 1422, 1637
BstAPI	GCANNNNNTGC	2 cut(s)	67, 544
BstBAI	YACGTR	1 cut(s)	1328
BstC8I	GCNNGC	4 cut(s)	174, 690, 969, 1260
BstDEI	CTNAG	9 cut(s)	180, 279, 434, 703, 759, 900, 1127, 1409, 1652
BstF5I	GGATG	9 cut(s)	19, 622, 948, 1099, 1123, 1170, 1399, 1446, 1702
BstFNI	CGCG	2 cut(s)	11, 1209
BstH2I	RGCGCY	1 cut(s)	326
BstHHI	GCGC	3 cut(s)	325, 880, 1243
BstKTI	GATC	9 cut(s)	466, 511, 574, 772, 886, 925, 1099, 1450, 1651
BstMAI	GTCTC	3 cut(s)	105, 393, 876
BstMBI	GATC	9 cut(s)	463, 508, 571, 769, 883, 922, 1096, 1447, 1648
BstMWI	GCNNNNNNNGC	6 cut(s)	67, 544, 739, 925, 973, 1268
BstNI	CCWGG	1 cut(s)	867
BstNSI	RCATGY	1 cut(s)	824
BstSCI	CCNGG	1 cut(s)	865
BstSFI	CTRYAG	4 cut(s)	59, 68, 318, 1626
BstSLI	GKGCMC	2 cut(s)	193, 570
BstUI	CGCG	2 cut(s)	11, 1209
BstV1I	GCAGC	5 cut(s)	45, 188, 720, 1009, 1687
BstV2I	GAAGAC	2 cut(s)	230, 290
BstX2I	RGATCY	2 cut(s)	883, 1648
BstXI	CCANNNNNNTGG	1 cut(s)	1715
BstYI	RGATCY	2 cut(s)	883, 1648
BstZ17I	GTATAC	1 cut(s)	154
BsuI	GTATCC	1 cut(s)	781
BsuRI	GGCC	2 cut(s)	1566, 1657
BtgZI	GCGATG	2 cut(s)	1127, 1224
BtsCI	GGATG	9 cut(s)	19, 622, 948, 1099, 1123, 1170, 1399, 1446, 1702
BtsIMutI	CAGTG	2 cut(s)	570, 1719
BtuMI	TCGCGA	1 cut(s)	1209
BveI	ACCTGC	1 cut(s)	1611
Cac8I	GCNNGC	4 cut(s)	174, 690, 969, 1260
CaiI	CAGNNNCTG	3 cut(s)	67, 733, 1022
CciI	TCATGA	1 cut(s)	505
CfoI	GCGC	3 cut(s)	325, 880, 1243
Cfr10I	RCCGGY	1 cut(s)	915
Cfr13I	GGNCC	3 cut(s)	1547, 1565, 1656
Csp6I	GTAC	4 cut(s)	584, 606, 654, 1459
CspCI	CAANNNNNGTGG	2 cut(s)	1309, 1344
CviAII	CATG	5 cut(s)	506, 821, 1001, 1090, 1405
CviQI	GTAC	4 cut(s)	584, 606, 654, 1459
DdeI	CTNAG	9 cut(s)	180, 279, 434, 703, 759, 900, 1127, 1409, 1652
DpnI	GATC	9 cut(s)	465, 510, 573, 771, 885, 924, 1098, 1449, 1650
DpnII	GATC	9 cut(s)	463, 508, 571, 769, 883, 922, 1096, 1447, 1648
DraIII	CACNNNGTG	1 cut(s)	544
Eam1104I	CTCTTC	4 cut(s)	1176, 1335, 1422, 1637
EarI	CTCTTC	4 cut(s)	1176, 1335, 1422, 1637
Ecl136II	GAGCTC	1 cut(s)	998
Eco24I	GRGCYC	1 cut(s)	1000
Eco31I	GGTCTC	1 cut(s)	876
Eco47I	GGWCC	1 cut(s)	1547
Eco53kI	GAGCTC	1 cut(s)	998
Eco57I	CTGAAG	6 cut(s)	110, 185, 273, 494, 828, 950
Eco72I	CACGTG	1 cut(s)	1328
Eco88I	CYCGRG	1 cut(s)	993
EcoICRI	GAGCTC	1 cut(s)	998
EcoO109I	RGGNCCY	1 cut(s)	1547
EcoRI	GAATTC	2 cut(s)	551, 1275
EcoRII	CCWGG	1 cut(s)	865
EcoT38I	GRGCYC	1 cut(s)	1000
FaeI	CATG	5 cut(s)	509, 824, 1004, 1093, 1408
FalI	AAGNNNNNCTT	2 cut(s)	129, 161
FaqI	GGGAC	1 cut(s)	307
FatI	CATG	5 cut(s)	505, 820, 1000, 1089, 1404
FbaI	TGATCA	1 cut(s)	1096
FblI	GTMKAC	2 cut(s)	153, 1305
Fnu4HI	GCNGC	5 cut(s)	59, 177, 734, 1023, 1701
FokI	GGATG	9 cut(s)	26, 609, 955, 1106, 1130, 1177, 1406, 1453, 1709
FriOI	GRGCYC	1 cut(s)	1000
Fsp4HI	GCNGC	5 cut(s)	59, 177, 734, 1023, 1701
FspAI	RTGCGCAY	1 cut(s)	1242
FspBI	CTAG	5 cut(s)	627, 684, 977, 1509, 1581
FspI	TGCGCA	1 cut(s)	1242
GlaI	GCGC	3 cut(s)	324, 879, 1242
GluI	GCNGC	5 cut(s)	59, 177, 734, 1023, 1701
GsaI	CCCAGC	1 cut(s)	423
HaeII	RGCGCY	1 cut(s)	326
HaeIII	GGCC	2 cut(s)	1566, 1657
HapII	CCGG	2 cut(s)	270, 916
HhaI	GCGC	3 cut(s)	325, 880, 1243
Hin1II	CATG	5 cut(s)	509, 824, 1004, 1093, 1408
Hin6I	GCGC	3 cut(s)	323, 878, 1241
HinP1I	GCGC	3 cut(s)	323, 878, 1241
HincII	GTYRAC	2 cut(s)	913, 1285
HindII	GTYRAC	2 cut(s)	913, 1285
HindIII	AAGCTT	1 cut(s)	740
HinfI	GANTC	7 cut(s)	215, 430, 458, 638, 749, 778, 1052
HpaI	GTTAAC	1 cut(s)	1285
HpaII	CCGG	2 cut(s)	270, 916
HphI	GGTGA	1 cut(s)	1731
Hpy166II	GTNNAC	6 cut(s)	154, 375, 568, 913, 1285, 1306
Hpy188I	TCNGA	7 cut(s)	214, 399, 463, 783, 888, 1101, 1452
Hpy188III	TCNNGA	8 cut(s)	219, 278, 506, 556, 601, 746, 961, 1208
Hpy8I	GTNNAC	6 cut(s)	154, 375, 568, 913, 1285, 1306
Hpy99I	CGWCG	1 cut(s)	1664
HpyAV	CCTTC	3 cut(s)	157, 361, 1531
HpyCH4III	ACNGT	6 cut(s)	443, 565, 610, 700, 862, 1729
HpyCH4IV	ACGT	3 cut(s)	1310, 1327, 1677
HpyCH4V	TGCA	7 cut(s)	61, 70, 538, 568, 736, 1262, 1556
HpyF10VI	GCNNNNNNNGC	6 cut(s)	67, 544, 739, 925, 973, 1268
HpyF3I	CTNAG	9 cut(s)	180, 279, 434, 703, 759, 900, 1127, 1409, 1652
HpySE526I	ACGT	3 cut(s)	1310, 1327, 1677
Hsp92II	CATG	5 cut(s)	509, 824, 1004, 1093, 1408
HspAI	GCGC	3 cut(s)	323, 878, 1241
Ksp22I	TGATCA	1 cut(s)	1096
KspAI	GTTAAC	1 cut(s)	1285
Kzo9I	GATC	9 cut(s)	463, 508, 571, 769, 883, 922, 1096, 1447, 1648
LguI	GCTCTTC	1 cut(s)	1335
LmnI	GCTCC	1 cut(s)	1669
Lsp1109I	GCAGC	5 cut(s)	45, 188, 720, 1009, 1687
LweI	GCATC	2 cut(s)	4, 1687
MaeI	CTAG	5 cut(s)	627, 684, 977, 1509, 1581
MaeII	ACGT	3 cut(s)	1310, 1327, 1677
MaeIII	GTNAC	4 cut(s)	610, 823, 830, 856
MalI	GATC	9 cut(s)	465, 510, 573, 771, 885, 924, 1098, 1449, 1650
MboI	GATC	9 cut(s)	463, 508, 571, 769, 883, 922, 1096, 1447, 1648
MflI	RGATCY	2 cut(s)	883, 1648
MhlI	GDGCHC	4 cut(s)	193, 563, 570, 1000
MlyI	GAGTC	3 cut(s)	467, 632, 758
MmeI	TCCRAC	1 cut(s)	13
MseI	TTAA	3 cut(s)	632, 1284, 1599
MslI	CAYNNNNRTG	2 cut(s)	1330, 1611
MspA1I	CMGCKG	2 cut(s)	340, 928
MspI	CCGG	2 cut(s)	270, 916
MspR9I	CCNGG	1 cut(s)	867
MvaI	CCWGG	1 cut(s)	867
MvnI	CGCG	2 cut(s)	11, 1209
MwoI	GCNNNNNNNGC	6 cut(s)	67, 544, 739, 925, 973, 1268
NdeII	GATC	9 cut(s)	463, 508, 571, 769, 883, 922, 1096, 1447, 1648
NlaIII	CATG	5 cut(s)	509, 824, 1004, 1093, 1408
NlaIV	GGNNCC	2 cut(s)	1567, 1731
NmuCI	GTSAC	1 cut(s)	856
NruI	TCGCGA	1 cut(s)	1209
NsbI	TGCGCA	1 cut(s)	1242
NspI	RCATGY	1 cut(s)	824
OliI	CACNNNNGTG	1 cut(s)	1330
PaeR7I	CTCGAG	1 cut(s)	993
PagI	TCATGA	1 cut(s)	505
PciI	ACATGT	1 cut(s)	820
PciSI	GCTCTTC	1 cut(s)	1335
PfeI	GAWTC	4 cut(s)	215, 430, 778, 1052
PkrI	GCNGC	5 cut(s)	60, 178, 735, 1024, 1702
PleI	GAGTC	3 cut(s)	466, 632, 757
PmaCI	CACGTG	1 cut(s)	1328
PmlI	CACGTG	1 cut(s)	1328
PpsI	GAGTC	3 cut(s)	466, 632, 757
Ppu21I	YACGTR	1 cut(s)	1328
PpuMI	RGGWCCY	1 cut(s)	1547
PscI	ACATGT	1 cut(s)	820
Psp124BI	GAGCTC	1 cut(s)	1000
Psp5II	RGGWCCY	1 cut(s)	1547
Psp6I	CCWGG	1 cut(s)	865
PspCI	CACGTG	1 cut(s)	1328
PspFI	CCCAGC	1 cut(s)	419
PspGI	CCWGG	1 cut(s)	865
PspN4I	GGNNCC	2 cut(s)	1567, 1731
PspPI	GGNCC	3 cut(s)	1547, 1565, 1656
PspPPI	RGGWCCY	1 cut(s)	1547
PspXI	VCTCGAGB	1 cut(s)	993
PstI	CTGCAG	2 cut(s)	63, 72
PstNI	CAGNNNCTG	3 cut(s)	67, 733, 1022
PsuI	RGATCY	2 cut(s)	883, 1648
PvuII	CAGCTG	2 cut(s)	340, 928
RruI	TCGCGA	1 cut(s)	1209
RsaI	GTAC	4 cut(s)	585, 607, 655, 1460
RsaNI	GTAC	4 cut(s)	584, 606, 654, 1459
RseI	CAYNNNNRTG	2 cut(s)	1330, 1611
SacI	GAGCTC	1 cut(s)	1000
SapI	GCTCTTC	1 cut(s)	1335
SaqAI	TTAA	3 cut(s)	632, 1284, 1599
SatI	GCNGC	5 cut(s)	59, 177, 734, 1023, 1701
Sau3AI	GATC	9 cut(s)	463, 508, 571, 769, 883, 922, 1096, 1447, 1648
Sau96I	GGNCC	3 cut(s)	1547, 1565, 1656
ScaI	AGTACT	1 cut(s)	607
SchI	GAGTC	3 cut(s)	467, 632, 758
ScrFI	CCNGG	1 cut(s)	867
SduI	GDGCHC	4 cut(s)	193, 563, 570, 1000
SfaNI	GCATC	2 cut(s)	4, 1687
SfcI	CTRYAG	4 cut(s)	59, 68, 318, 1626
Sfr274I	CTCGAG	1 cut(s)	993
SinI	GGWCC	1 cut(s)	1547
SlaI	CTCGAG	1 cut(s)	993
SmiMI	CAYNNNNRTG	2 cut(s)	1330, 1611
SmlI	CTYRAG	4 cut(s)	601, 993, 1193, 1253
SmoI	CTYRAG	4 cut(s)	601, 993, 1193, 1253
SsiI	CCGC	2 cut(s)	11, 449
SspMI	CTAG	5 cut(s)	627, 684, 977, 1509, 1581
SstI	GAGCTC	1 cut(s)	1000
StyD4I	CCNGG	1 cut(s)	865
TaaI	ACNGT	6 cut(s)	443, 565, 610, 700, 862, 1729
TaiI	ACGT	3 cut(s)	1313, 1330, 1680
TaqI	TCGA	6 cut(s)	168, 428, 921, 994, 1065, 1519
TatI	WGTACW	2 cut(s)	583, 605
TfiI	GAWTC	4 cut(s)	215, 430, 778, 1052
Tru1I	TTAA	3 cut(s)	632, 1284, 1599
Tru9I	TTAA	3 cut(s)	632, 1284, 1599
TscAI	CASTG	2 cut(s)	570, 1726
TseFI	GTSAC	1 cut(s)	856
TseI	GCWGC	5 cut(s)	58, 176, 733, 1022, 1700
Tsp45I	GTSAC	1 cut(s)	856
TspGWI	ACGGA	1 cut(s)	1450
TspRI	CASTG	2 cut(s)	570, 1726
VneI	GTGCAC	1 cut(s)	566
VpaK11BI	GGWCC	1 cut(s)	1547
XapI	RAATTY	6 cut(s)	75, 393, 488, 551, 1275, 1290
XceI	RCATGY	1 cut(s)	824
XhoI	CTCGAG	1 cut(s)	993
XmiI	GTMKAC	2 cut(s)	153, 1305
XspI	CTAG	5 cut(s)	627, 684, 977, 1509, 1581
ZrmI	AGTACT	1 cut(s)	607

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.