Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0008209

Physical Location & Seq

Genomic Coordinates Reverse (-)

39750723 .. 39755068

Gene Structure 4346 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1280100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1845 bp

ATGATGGAGCAGTATGAAGTTCTGGAGCAGATTGGGAAGGGTTCTTTTGGTTCTGCTCTTCTCGTGAGGCATAAGCACGAAAACAAGAAGTATGTCCTTAAGAAGATCCGTCTTGCTCGTCAAACTGATAGAAGCCGGAGATCTGCTCACCAGGAGATGGAGCTTATTTCCACGGTACAGAATCCGTTCATCGTGGAGTACAAAGATTCCTGGGTTGAAAGGGGTTGCTTTGTGTGCATTGTTATAGGGTATTGTGAAGGAGGAGACATGATGGAAGCCATAAAAAGAGCCCGTGGTGTTCATTTCTCAGAAGAGAAACTCTGCCAATGGCTAGTTCAACTGTTGATGGCACTTGATTACTTGCATGCAAACCATATTCTTCACCGTGATGTCAAGTGTTCAAATATATTTTTGACAAAAGATCAAGACATACGTCTGGGTGACTTTGGTCTCGCGAAAATGTTAACTTCGGACGATCTAGCTTCCTCTGTAGTCGGAACTCCCAGTTATATGTGCCCTGAGCTTCTTGCTGACATACCTTATGGTTCTAAGTCAGATATATGGTCTTTAGGATGCTGTATATATGAAATGGCTGCGTTCAGGCCTGCATTCAAAGCCTTTGATATACAATCTCTGATTAACAAAATAAACAAGTCAATAGTGGCTCCACTTCCAACGTTGTACTCTGGTGCATTTCGAGGTCTTGTTAAAAGCATGCTGCGGAAGAACCCAGAACTTAGGCCAAGTGCTGCAGAGGTGCTTGCTCATCCCCATCTTCAGCCTTATGTGCTTAAGATTCATCTAAAAGTGAATATCCCTCGGCGGAATACTTTCCCAGTTGACTGGTCTCGGTCCAGTTATGTGAGGAAAACACGATTCATGGAGCCAGAAACTAGTCCTGTTCGTACCAGCAGAGAGAAAAGACGATCATTCAGCAATGATAGGACCTTAAATCCTAGTATCTCTGGAACTGAACAGGGCTCTCCAAGTTCTAGCGTGCGGACACTGGACTTTCCAGGGTGTTTAAGTCGTAAGTTCACCGAGGTATCTATGGGAAGTACCAAGGAAGATTATGGTAACAGAAAGTTGGTGTCCACAAAGTACAATGTTGTTAATAGTCCACGAGCAGCACCAGCAAAAGTTTCTGCTACTCCCACGAAACACATTACAGAGTCAAGAATTTCTCACGTCAACTCAAAACGTGATTCGCTTCCGGTGTCACATTCTCCAGCCAGAAAATCTTCCCAGTCAACTCGAAGAGCTTCTCTTCCGTTCTTGACAAGTACTGCAGCTCCACAAACCCCATATAGAGCCAATGTTGGTCCCCTTGGGATTATGGAGTCTCCAGACGTTTCTGTCAATGCCCCGCGAATTGACAAGATGGCTGAATTCCCCTTAGCCTCTTCTGAAGAGCCACTCGTTCCTACCCGTGGAATTTCATCAACATCTGCACGATGCCCTTCAACCAAGCTGAATAGCATCAACCATTCTATCACAAAGGACAAGTGCATGATCCAGGTTATAGACAAAACCATTTCCAAGTCCAGTACAATTGATGGCAGCCATGGACCTGCTCGAAATGGCAGTGAATCTCCTGAGCAGAAAACTACCGCTGGTGTTTCGAGCCATTCATCTTCGGAGTCACGACAGCAGCGATTCGACACCTCATCATTCCAGCAACGTGCAGAGGCTTTGGAAGGGTTGCTTGAGTTCAGCGCGCGTCTTCTACAACAAGAAAGGTTCGAAGAGCTTGGAGTGCTCTTGAAGCCATTCGGACCCGAGAAGGTTTCTCCGAGGGAAACAGCCATTTGGTTGACCAAGAGCTTCAAAGAAACCACGGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

615

Amino Acids

68.55

Weight (kDa)

9.51

Isoelectric Point (pI)

54.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	5 - 258	7.4e-64	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	7 - 254	1.9e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63280 AT3G63280 AT3G63280 AT3G63280
fragaria_vesca	FvH4_2g23090
malus_domestica	MD03G1259600.v1.1 MD11G1280100.v1.1
prunus_persica	Prupe.8G236000_v2.0.a1 Prupe.8G236000_v2.0.a1
pyrus_communis	pycom03g20750 pycom11g24780
rosa_chinensis	RchiOBHm_Chr6g0290241 RchiOBHm_Chr7g0178391
rosa_laevigata	RLG00000012231
rosa_multiflora	Rmu_sc0002902.1_g000010
rosa_rugosa	Rorug06G0211200
rosa_samantha	Rh6AG321500 Rh6BG328600 Rh6CG334600 Rh6CG334700 Rh6DG321600
rosa_wichuraiana	Rw4G037780 Rw6G027770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1355
Acc36I	ACCTGC	1 cut(s)	1579
AccB7I	CCANNNNNTGG	1 cut(s)	157
AccII	CGCG	4 cut(s)	455, 1369, 1718, 1720
AciI	CCGC	5 cut(s)	721, 823, 1000, 1367, 1611
AclI	AACGTT	1 cut(s)	677
AclWI	GGATC	2 cut(s)	100, 1507
AcsI	RAATTY	3 cut(s)	1179, 1388, 1434
AcuI	CTGAAG	2 cut(s)	761, 1428
AfaI	GTAC	8 cut(s)	177, 200, 683, 907, 1060, 1103, 1285, 1549
AfiI	CCNNNNNNNGG	2 cut(s)	157, 1430
AflII	CTTAAG	2 cut(s)	98, 791
AgsI	TTSAA	7 cut(s)	218, 338, 402, 613, 1464, 1765, 1828
AhlI	ACTAGT	1 cut(s)	893
AjiI	CACGTC	1 cut(s)	1189
AjnI	CCWGG	4 cut(s)	150, 209, 1015, 1515
AjuI	GAANNNNNNNTTGG	2 cut(s)	318, 350
AluBI	AGCT	8 cut(s)	163, 482, 523, 1262, 1292, 1471, 1750, 1824
AluI	AGCT	8 cut(s)	163, 482, 523, 1262, 1292, 1471, 1750, 1824
Alw21I	GWGCWC	1 cut(s)	1761
Alw26I	GTCTC	4 cut(s)	258, 455, 852, 1347
AlwI	GGATC	2 cut(s)	100, 1507
Ama87I	CYCGRG	1 cut(s)	1778
AoxI	GGCC	2 cut(s)	602, 740
ApeKI	GCWGC	7 cut(s)	593, 718, 749, 1127, 1289, 1560, 1651
ApoI	RAATTY	3 cut(s)	1179, 1388, 1434
Asp700I	GAANNNNTTC	5 cut(s)	185, 830, 1240, 1261, 1769
AspLEI	GCGC	2 cut(s)	1718, 1720
AspS9I	GGNCC	5 cut(s)	852, 945, 1322, 1568, 1775
AsuHPI	GGTGA	4 cut(s)	140, 374, 452, 1030
AsuII	TTCGAA	1 cut(s)	1743
AvaI	CYCGRG	1 cut(s)	1778
AvaII	GGWCC	5 cut(s)	852, 945, 1322, 1568, 1775
BaeGI	GKGCMC	1 cut(s)	518
BaeI	ACNNNNGTAYC	2 cut(s)	1029, 1062
BanII	GRGCYC	2 cut(s)	292, 983
BauI	CACGAG	2 cut(s)	62, 1122
BbsI	GAAGAC	1 cut(s)	1715
Bbv12I	GWGCWC	1 cut(s)	1761
BbvI	GCAGC	7 cut(s)	580, 705, 736, 1139, 1301, 1572, 1663
BccI	CCATC	6 cut(s)	151, 265, 340, 780, 1375, 1550
BciT130I	CCWGG	4 cut(s)	152, 211, 1017, 1517
BcoDI	GTCTC	4 cut(s)	258, 455, 852, 1347
BcuI	ACTAGT	1 cut(s)	893
BfaI	CTAG	5 cut(s)	332, 479, 894, 957, 993
BfmI	CTRYAG	3 cut(s)	489, 750, 1287
BfrI	CTTAAG	2 cut(s)	98, 791
BfuAI	ACCTGC	1 cut(s)	1579
BglII	AGATCT	1 cut(s)	140
BisI	GCNGC	7 cut(s)	594, 719, 750, 1128, 1290, 1561, 1652
BlsI	GCNGC	7 cut(s)	595, 720, 751, 1129, 1291, 1562, 1653
BmcAI	AGTACT	1 cut(s)	1285
Bme1390I	CCNGG	4 cut(s)	152, 211, 1017, 1517
Bme18I	GGWCC	5 cut(s)	852, 945, 1322, 1568, 1775
BmeT110I	CYCGRG	1 cut(s)	1778
BmgBI	CACGTC	1 cut(s)	1189
BmgT120I	GGNCC	5 cut(s)	852, 945, 1322, 1568, 1775
BmiI	GGNNCC	4 cut(s)	666, 885, 1324, 1777
BmrFI	CCNGG	4 cut(s)	152, 211, 1017, 1517
BmrI	ACTGGG	3 cut(s)	498, 830, 1240
BmsI	GCATC	3 cut(s)	563, 1445, 1488
BmuI	ACTGGG	3 cut(s)	498, 830, 1240
BoxI	GACNNNNGTC	2 cut(s)	432, 447
BpiI	GAAGAC	1 cut(s)	1715
BplI	GAGNNNNNCTC	2 cut(s)	130, 162
BpmI	CTGGAG	3 cut(s)	44, 1212, 1329
Bpu10I	CCTNAGC	3 cut(s)	519, 1396, 1596
Bpu14I	TTCGAA	1 cut(s)	1743
BpuEI	CTTGAG	1 cut(s)	1727
BsaI	GGTCTC	2 cut(s)	455, 852
BsaWI	WCCGGW	1 cut(s)	1213
BsaXI	ACNNNNNCTCC	2 cut(s)	1276, 1306
Bsc4I	CCNNNNNNNGG	2 cut(s)	157, 1430
Bse1I	ACTGG	7 cut(s)	504, 836, 848, 855, 1011, 1246, 1545
Bse3DI	GCAATG	1 cut(s)	943
BseBI	CCWGG	4 cut(s)	152, 211, 1017, 1517
BseGI	GGATG	2 cut(s)	578, 766
BseLI	CCNNNNNNNGG	2 cut(s)	157, 1430
BseMI	GCAATG	1 cut(s)	943
BseMII	CTCAG	3 cut(s)	321, 510, 1587
BseNI	ACTGG	7 cut(s)	504, 836, 848, 855, 1011, 1246, 1545
BsePI	GCGCGC	1 cut(s)	1716
BseRI	GAGGAG	1 cut(s)	276
BseSI	GKGCMC	1 cut(s)	518
BseXI	GCAGC	7 cut(s)	580, 705, 736, 1139, 1301, 1572, 1663
BsgI	GTGCAG	2 cut(s)	1434, 1704
Bsh1236I	CGCG	4 cut(s)	455, 1369, 1718, 1720
BshFI	GGCC	2 cut(s)	604, 742
BsiHKAI	GWGCWC	1 cut(s)	1761
BsiHKCI	CYCGRG	1 cut(s)	1778
BsiSI	CCGG	2 cut(s)	136, 1214
BslFI	GGGAC	1 cut(s)	1308
BslI	CCNNNNNNNGG	2 cut(s)	157, 1430
BsmAI	GTCTC	4 cut(s)	258, 455, 852, 1347
BsmFI	GGGAC	1 cut(s)	1308
BsmI	GAATGC	1 cut(s)	608
BsnI	GGCC	2 cut(s)	604, 742
Bso31I	GGTCTC	2 cut(s)	455, 852
BsoBI	CYCGRG	1 cut(s)	1778
Bsp119I	TTCGAA	1 cut(s)	1743
Bsp1286I	GDGCHC	4 cut(s)	292, 518, 983, 1761
Bsp143I	GATC	6 cut(s)	105, 140, 421, 475, 926, 1512
Bsp19I	CCATGG	1 cut(s)	1564
Bsp68I	TCGCGA	1 cut(s)	455
BspACI	CCGC	5 cut(s)	721, 823, 1000, 1367, 1611
BspANI	GGCC	2 cut(s)	604, 742
BspCNI	CTCAG	3 cut(s)	320, 511, 1588
BspFNI	CGCG	4 cut(s)	455, 1369, 1718, 1720
BspLI	GGNNCC	4 cut(s)	666, 885, 1324, 1777
BspMAI	CTGCAG	2 cut(s)	754, 1291
BspMI	ACCTGC	1 cut(s)	1579
BspPI	GGATC	2 cut(s)	100, 1507
BspQI	GCTCTTC	4 cut(s)	63, 1252, 1404, 1740
BspT104I	TTCGAA	1 cut(s)	1743
BspTI	CTTAAG	2 cut(s)	98, 791
BspTNI	GGTCTC	2 cut(s)	455, 852
BsrDI	GCAATG	1 cut(s)	943
BsrI	ACTGG	7 cut(s)	504, 836, 848, 855, 1011, 1246, 1545
BssHII	GCGCGC	1 cut(s)	1716
BssMI	GATC	6 cut(s)	105, 140, 421, 475, 926, 1512
BssSI	CACGAG	2 cut(s)	62, 1122
BssT1I	CCWWGG	3 cut(s)	1062, 1327, 1564
Bst2BI	CACGAG	2 cut(s)	62, 1122
Bst2UI	CCWGG	4 cut(s)	152, 211, 1017, 1517
Bst4CI	ACNGT	3 cut(s)	175, 342, 386
Bst6I	CTCTTC	7 cut(s)	63, 306, 1252, 1272, 1404, 1408, 1740
BstAFI	CTTAAG	2 cut(s)	98, 791
BstBI	TTCGAA	1 cut(s)	1743
BstC8I	GCNNGC	6 cut(s)	366, 606, 716, 762, 998, 1718
BstDEI	CTNAG	7 cut(s)	307, 519, 549, 737, 1396, 1596, 1842
BstDSI	CCRYGG	5 cut(s)	171, 292, 1429, 1564, 1836
BstF5I	GGATG	2 cut(s)	578, 766
BstFNI	CGCG	4 cut(s)	455, 1369, 1718, 1720
BstHHI	GCGC	2 cut(s)	1718, 1720
BstKTI	GATC	6 cut(s)	108, 143, 424, 478, 929, 1515
BstMAI	GTCTC	4 cut(s)	258, 455, 852, 1347
BstMBI	GATC	6 cut(s)	105, 140, 421, 475, 926, 1512
BstMWI	GCNNNNNNNGC	6 cut(s)	234, 614, 787, 1133, 1756, 1765
BstNI	CCWGG	4 cut(s)	152, 211, 1017, 1517
BstNSI	RCATGY	2 cut(s)	368, 718
BstPAI	GACNNNNGTC	2 cut(s)	432, 447
BstSCI	CCNGG	4 cut(s)	150, 209, 1015, 1515
BstSFI	CTRYAG	3 cut(s)	489, 750, 1287
BstSLI	GKGCMC	1 cut(s)	518
BstUI	CGCG	4 cut(s)	455, 1369, 1718, 1720
BstV1I	GCAGC	7 cut(s)	580, 705, 736, 1139, 1301, 1572, 1663
BstV2I	GAAGAC	1 cut(s)	1715
BstX2I	RGATCY	2 cut(s)	105, 140
BstXI	CCANNNNNNTGG	1 cut(s)	843
BstYI	RGATCY	2 cut(s)	105, 140
BsuRI	GGCC	2 cut(s)	604, 742
BtgI	CCRYGG	5 cut(s)	171, 292, 1429, 1564, 1836
BtrI	CACGTC	1 cut(s)	1189
BtsCI	GGATG	2 cut(s)	578, 766
BtsI	GCAGTG	1 cut(s)	1591
BtsIMutI	CAGTG	2 cut(s)	1004, 1591
BtuMI	TCGCGA	1 cut(s)	455
BveI	ACCTGC	1 cut(s)	1579
Cac8I	GCNNGC	6 cut(s)	366, 606, 716, 762, 998, 1718
CfoI	GCGC	2 cut(s)	1718, 1720
Cfr13I	GGNCC	5 cut(s)	852, 945, 1322, 1568, 1775
CseI	GACGC	1 cut(s)	1709
Csp6I	GTAC	8 cut(s)	176, 199, 682, 906, 1059, 1102, 1284, 1548
CviAII	CATG	6 cut(s)	268, 365, 715, 880, 1510, 1565
CviQI	GTAC	8 cut(s)	176, 199, 682, 906, 1059, 1102, 1284, 1548
DdeI	CTNAG	7 cut(s)	307, 519, 549, 737, 1396, 1596, 1842
DpnI	GATC	6 cut(s)	107, 142, 423, 477, 928, 1514
DpnII	GATC	6 cut(s)	105, 140, 421, 475, 926, 1512
DrdI	GACNNNNNNGTC	1 cut(s)	1355
DseDI	GACNNNNNNGTC	1 cut(s)	1355
Eam1104I	CTCTTC	7 cut(s)	63, 306, 1252, 1272, 1404, 1408, 1740
EarI	CTCTTC	7 cut(s)	63, 306, 1252, 1272, 1404, 1408, 1740
EciI	GGCGGA	1 cut(s)	838
Eco130I	CCWWGG	3 cut(s)	1062, 1327, 1564
Eco147I	AGGCCT	1 cut(s)	604
Eco24I	GRGCYC	2 cut(s)	292, 983
Eco31I	GGTCTC	2 cut(s)	455, 852
Eco47I	GGWCC	5 cut(s)	852, 945, 1322, 1568, 1775
Eco57I	CTGAAG	2 cut(s)	761, 1428
Eco88I	CYCGRG	1 cut(s)	1778
EcoO109I	RGGNCCY	1 cut(s)	945
EcoRI	GAATTC	1 cut(s)	1388
EcoRII	CCWGG	4 cut(s)	150, 209, 1015, 1515
EcoT14I	CCWWGG	3 cut(s)	1062, 1327, 1564
EcoT38I	GRGCYC	2 cut(s)	292, 983
ErhI	CCWWGG	3 cut(s)	1062, 1327, 1564
FaeI	CATG	6 cut(s)	271, 368, 718, 883, 1513, 1568
FalI	AAGNNNNNCTT	2 cut(s)	1689, 1721
FaqI	GGGAC	1 cut(s)	1308
FatI	CATG	6 cut(s)	267, 364, 714, 879, 1509, 1564
FauI	CCCGC	1 cut(s)	1374
Fnu4HI	GCNGC	7 cut(s)	594, 719, 750, 1128, 1290, 1561, 1652
FokI	GGATG	2 cut(s)	585, 753
FriOI	GRGCYC	2 cut(s)	292, 983
Fsp4HI	GCNGC	7 cut(s)	594, 719, 750, 1128, 1290, 1561, 1652
FspBI	CTAG	5 cut(s)	332, 479, 894, 957, 993
GlaI	GCGC	2 cut(s)	1717, 1719
GluI	GCNGC	7 cut(s)	594, 719, 750, 1128, 1290, 1561, 1652
GsuI	CTGGAG	3 cut(s)	44, 1212, 1329
HaeIII	GGCC	2 cut(s)	604, 742
HapII	CCGG	2 cut(s)	136, 1214
HgaI	GACGC	1 cut(s)	1709
HhaI	GCGC	2 cut(s)	1718, 1720
Hin1II	CATG	6 cut(s)	271, 368, 718, 883, 1513, 1568
Hin6I	GCGC	2 cut(s)	1716, 1718
HinP1I	GCGC	2 cut(s)	1716, 1718
HincII	GTYRAC	5 cut(s)	465, 841, 1192, 1251, 1815
HindII	GTYRAC	5 cut(s)	465, 841, 1192, 1251, 1815
HpaI	GTTAAC	1 cut(s)	465
HpaII	CCGG	2 cut(s)	136, 1214
HphI	GGTGA	4 cut(s)	140, 374, 452, 1030
Hpy166II	GTNNAC	8 cut(s)	465, 841, 1038, 1095, 1121, 1192, 1251, 1815
Hpy188I	TCNGA	9 cut(s)	310, 472, 497, 556, 636, 1408, 1639, 1775, 1794
Hpy8I	GTNNAC	8 cut(s)	465, 841, 1038, 1095, 1121, 1192, 1251, 1815
HpyAV	CCTTC	5 cut(s)	31, 251, 1470, 1691, 1777
HpyCH4III	ACNGT	3 cut(s)	175, 342, 386
HpyCH4IV	ACGT	6 cut(s)	433, 677, 1188, 1201, 1350, 1681
HpyF10VI	GCNNNNNNNGC	6 cut(s)	234, 614, 787, 1133, 1756, 1765
HpyF3I	CTNAG	7 cut(s)	307, 519, 549, 737, 1396, 1596, 1842
HpySE526I	ACGT	6 cut(s)	433, 677, 1188, 1201, 1350, 1681
Hsp92II	CATG	6 cut(s)	271, 368, 718, 883, 1513, 1568
HspAI	GCGC	2 cut(s)	1716, 1718
KspAI	GTTAAC	1 cut(s)	465
Kzo9I	GATC	6 cut(s)	105, 140, 421, 475, 926, 1512
LguI	GCTCTTC	4 cut(s)	63, 1252, 1404, 1740
LmnI	GCTCC	6 cut(s)	7, 25, 160, 670, 883, 1297
Lsp1109I	GCAGC	7 cut(s)	580, 705, 736, 1139, 1301, 1572, 1663
LweI	GCATC	3 cut(s)	563, 1445, 1488
MaeI	CTAG	5 cut(s)	332, 479, 894, 957, 993
MaeII	ACGT	6 cut(s)	433, 677, 1188, 1201, 1350, 1681
MaeIII	GTNAC	4 cut(s)	440, 1076, 1218, 1641
MalI	GATC	6 cut(s)	107, 142, 423, 477, 928, 1514
MboI	GATC	6 cut(s)	105, 140, 421, 475, 926, 1512
MfeI	CAATTG	1 cut(s)	1551
MflI	RGATCY	2 cut(s)	105, 140
MhlI	GDGCHC	4 cut(s)	292, 518, 983, 1761
MluCI	AATT	5 cut(s)	1179, 1371, 1388, 1434, 1551
MlyI	GAGTC	3 cut(s)	1181, 1349, 1649
MmeI	TCCRAC	2 cut(s)	475, 698
MroXI	GAANNNNTTC	5 cut(s)	185, 830, 1240, 1261, 1769
MseI	TTAA	8 cut(s)	99, 464, 639, 708, 792, 950, 1025, 1113
MslI	CAYNNNNRTG	1 cut(s)	387
MspA1I	CMGCKG	1 cut(s)	1613
MspCI	CTTAAG	2 cut(s)	98, 791
MspI	CCGG	2 cut(s)	136, 1214
MspR9I	CCNGG	4 cut(s)	152, 211, 1017, 1517
MunI	CAATTG	1 cut(s)	1551
Mva1269I	GAATGC	1 cut(s)	608
MvaI	CCWGG	4 cut(s)	152, 211, 1017, 1517
MvnI	CGCG	4 cut(s)	455, 1369, 1718, 1720
MwoI	GCNNNNNNNGC	6 cut(s)	234, 614, 787, 1133, 1756, 1765
NcoI	CCATGG	1 cut(s)	1564
NdeII	GATC	6 cut(s)	105, 140, 421, 475, 926, 1512
NlaIII	CATG	6 cut(s)	271, 368, 718, 883, 1513, 1568
NlaIV	GGNNCC	4 cut(s)	666, 885, 1324, 1777
NmeAIII	GCCGAG	1 cut(s)	799
NmuCI	GTSAC	3 cut(s)	440, 1218, 1641
NruI	TCGCGA	1 cut(s)	455
NspI	RCATGY	2 cut(s)	368, 718
NspV	TTCGAA	1 cut(s)	1743
PaeI	GCATGC	2 cut(s)	368, 718
PauI	GCGCGC	1 cut(s)	1716
PceI	AGGCCT	1 cut(s)	604
PciSI	GCTCTTC	4 cut(s)	63, 1252, 1404, 1740
PcsI	WCGNNNNNNNCGW	1 cut(s)	1651
PctI	GAATGC	1 cut(s)	608
PdmI	GAANNNNTTC	5 cut(s)	185, 830, 1240, 1261, 1769
PfeI	GAWTC	7 cut(s)	181, 206, 796, 876, 1205, 1589, 1656
PflMI	CCANNNNNTGG	1 cut(s)	157
PkrI	GCNGC	7 cut(s)	595, 720, 751, 1129, 1291, 1562, 1653
PleI	GAGTC	3 cut(s)	1180, 1348, 1648
PpsI	GAGTC	3 cut(s)	1180, 1348, 1648
PpuMI	RGGWCCY	1 cut(s)	945
PshAI	GACNNNNGTC	2 cut(s)	432, 447
Psp1406I	AACGTT	1 cut(s)	677
Psp5II	RGGWCCY	1 cut(s)	945
Psp6I	CCWGG	4 cut(s)	150, 209, 1015, 1515
PspGI	CCWGG	4 cut(s)	150, 209, 1015, 1515
PspN4I	GGNNCC	4 cut(s)	666, 885, 1324, 1777
PspPI	GGNCC	5 cut(s)	852, 945, 1322, 1568, 1775
PspPPI	RGGWCCY	1 cut(s)	945
PstI	CTGCAG	2 cut(s)	754, 1291
PsuI	RGATCY	2 cut(s)	105, 140
PteI	GCGCGC	1 cut(s)	1716
RruI	TCGCGA	1 cut(s)	455
RsaI	GTAC	8 cut(s)	177, 200, 683, 907, 1060, 1103, 1285, 1549
RsaNI	GTAC	8 cut(s)	176, 199, 682, 906, 1059, 1102, 1284, 1548
RseI	CAYNNNNRTG	1 cut(s)	387
SapI	GCTCTTC	4 cut(s)	63, 1252, 1404, 1740
SaqAI	TTAA	8 cut(s)	99, 464, 639, 708, 792, 950, 1025, 1113
SatI	GCNGC	7 cut(s)	594, 719, 750, 1128, 1290, 1561, 1652
Sau3AI	GATC	6 cut(s)	105, 140, 421, 475, 926, 1512
Sau96I	GGNCC	5 cut(s)	852, 945, 1322, 1568, 1775
ScaI	AGTACT	1 cut(s)	1285
SchI	GAGTC	3 cut(s)	1181, 1349, 1649
ScrFI	CCNGG	4 cut(s)	152, 211, 1017, 1517
SduI	GDGCHC	4 cut(s)	292, 518, 983, 1761
SfaNI	GCATC	3 cut(s)	563, 1445, 1488
SfcI	CTRYAG	3 cut(s)	489, 750, 1287
SfuI	TTCGAA	1 cut(s)	1743
SinI	GGWCC	5 cut(s)	852, 945, 1322, 1568, 1775
SmiMI	CAYNNNNRTG	1 cut(s)	387
SmlI	CTYRAG	3 cut(s)	98, 791, 1706
SmoI	CTYRAG	3 cut(s)	98, 791, 1706
SpeI	ACTAGT	1 cut(s)	893
SphI	GCATGC	2 cut(s)	368, 718
Sse9I	AATT	5 cut(s)	1179, 1371, 1388, 1434, 1551
SseBI	AGGCCT	1 cut(s)	604
SsiI	CCGC	5 cut(s)	721, 823, 1000, 1367, 1611
SspMI	CTAG	5 cut(s)	332, 479, 894, 957, 993
StuI	AGGCCT	1 cut(s)	604
StyD4I	CCNGG	4 cut(s)	150, 209, 1015, 1515
StyI	CCWWGG	3 cut(s)	1062, 1327, 1564
TaaI	ACNGT	3 cut(s)	175, 342, 386
TaiI	ACGT	6 cut(s)	436, 680, 1191, 1204, 1353, 1684
TaqI	TCGA	6 cut(s)	697, 1255, 1576, 1622, 1659, 1743
TaqII	GACCGA	1 cut(s)	840
TasI	AATT	5 cut(s)	1179, 1371, 1388, 1434, 1551
TatI	WGTACW	5 cut(s)	198, 681, 1101, 1283, 1547
TfiI	GAWTC	7 cut(s)	181, 206, 796, 876, 1205, 1589, 1656
Tru1I	TTAA	8 cut(s)	99, 464, 639, 708, 792, 950, 1025, 1113
Tru9I	TTAA	8 cut(s)	99, 464, 639, 708, 792, 950, 1025, 1113
TscAI	CASTG	2 cut(s)	1011, 1591
TseFI	GTSAC	3 cut(s)	440, 1218, 1641
TseI	GCWGC	7 cut(s)	593, 718, 749, 1127, 1289, 1560, 1651
Tsp45I	GTSAC	3 cut(s)	440, 1218, 1641
TspDTI	ATGAA	8 cut(s)	30, 178, 290, 600, 788, 868, 1428, 1620
TspGWI	ACGGA	3 cut(s)	98, 174, 1260
TspRI	CASTG	2 cut(s)	1011, 1591
Van91I	CCANNNNNTGG	1 cut(s)	157
Vha464I	CTTAAG	2 cut(s)	98, 791
VpaK11BI	GGWCC	5 cut(s)	852, 945, 1322, 1568, 1775
XapI	RAATTY	3 cut(s)	1179, 1388, 1434
XceI	RCATGY	2 cut(s)	368, 718
XmnI	GAANNNNTTC	5 cut(s)	185, 830, 1240, 1261, 1769
XspI	CTAG	5 cut(s)	332, 479, 894, 957, 993
ZrmI	AGTACT	1 cut(s)	1285

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.