Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0019576

Physical Location & Seq

Genomic Coordinates Forward (+)

172089 .. 174165

Gene Structure 2077 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1000800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1128 bp

ATGAAAAACCCAGAATCAAGCCTGCCACCAGGATTTAGGTTTCACCCTACAGATGAAGAGCTCATCCTTCACTACCTTGTAAAGAAGGTGGCCTGCACTCCCTTACCTGTTTCCGTCATCGCTGAAGTTGATATCTACAAGTTTGATCCATGGCAATTGCCAGCCAAAGCTGCGTTTGGTGAGAAAGAATGGTACTTCTTCAGTCCAAGAGATCGCAAGTACCCGAATGGTGCGAGGCCAAACAGGGCTGCCGCATCAGGGTATTGGAAGGCAACTGGAACCGATAAGACGATTGTGGCACCATCAGGACGGCGGCAGAACGTTGGTGTGAAGAAGGCTCTTGTGTTCTACAAGGGGAGACCCCCTAAGGGAATCAAGACAAACTGGATCATGCATGAGTATCGCCTTCCAGAAAACCCAAATAACCACACCACCACCAACAACCACCACAGAGCCATGAAGCTCAAAGATTTATCCATGAGGTTGGATGATTGGGTTCTTTGTCGAATCTACAAGAAGTCGAATGTTGTGACTTCAGCAGTGGCACTGCCAAGTGATCAAAACCAAGAAGTTCAAGAAGAAGAAGATTTCCTACACGATATCCTTTCACCAAGCTTGAAATGTCCCTTTACCGGCCTTGATCACAACATCATGAGTACCAGTAATACTAGCAGTCTCAAGCCTCAGAAATCTTGTTCCTTCTCCAACTTACCAGACGCCATGGACTACTCATTACTCACCAGTATTCTGGCGGATGGCCAATACTCTATCCCAACTGGAATTGGATTTCAATCAACTACTCCTAATAAGTTTAGCTATAGCTGTACCGGGAGTACTGGTATCGGTTTAGACCCGCAACCGCTGTTCAATACCGATATTAGCAGCAACAGCAGCAGCAGTCATTTGCTTCAAACTCTACCTCAATACAACGGTTTGAACTTGCCATCATCAGCTCCAAGCACCGAAAACCGGCTCAAGCGCCAGCATTCCATCACAGATGATCGTGGCGTGTTATTTTGTCCATCGAAGAAATACGTAAACTCCAACTGCAGTTTCACTAACACCGCCAATTCATTCTTGAATCAGCAATTACTTCTGAGTAGTCCTAGTTTTCATTTTCAAGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009825 GO:0009835 GO:0009889 GO:0009908 GO:0009987 GO:0010150 GO:0010154 GO:0010468 GO:0010556 GO:0016049 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048366 GO:0048367 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071695 GO:0080090 GO:0090567 GO:0090693 GO:0099402 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAM

Protein Analysis

376

Amino Acids

41.97

Weight (kDa)

8.91

Isoelectric Point (pI)

45.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAM	PF02365	9 - 136	1.8e-50	No apical meristem (NAM) protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0019576)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04070 AT3G04070
malus_domestica	MD12G1000800.v1.1 MD14G1001000.v1.1
prunus_persica	Prupe.7G001200_v2.0.a1
pyrus_communis	pycom12g00080 pycom49g00050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	298
AciI	CCGC	6 cut(s)	252, 313, 752, 854, 860, 1065
AclI	AACGTT	1 cut(s)	321
AclWI	GGATC	2 cut(s)	140, 395
AcoI	YGGCCR	1 cut(s)	757
AcuI	CTGAAG	3 cut(s)	144, 184, 519
AcyI	GRCGYC	1 cut(s)	717
AfaI	GTAC	5 cut(s)	194, 221, 658, 826, 835
AfiI	CCNNNNNNNGG	4 cut(s)	258, 368, 632, 969
AgsI	TTSAA	8 cut(s)	575, 619, 791, 868, 911, 937, 1081, 1121
AjnI	CCWGG	1 cut(s)	28
AjuI	GAANNNNNNNTTGG	2 cut(s)	1037, 1069
AluBI	AGCT	7 cut(s)	61, 170, 463, 615, 816, 822, 953
AluI	AGCT	7 cut(s)	61, 170, 463, 615, 816, 822, 953
Alw21I	GWGCWC	1 cut(s)	63
Alw26I	GTCTC	2 cut(s)	352, 680
AlwI	GGATC	2 cut(s)	140, 395
AoxI	GGCC	4 cut(s)	90, 236, 634, 757
ApeKI	GCWGC	5 cut(s)	170, 248, 882, 891, 894
AspLEI	GCGC	1 cut(s)	981
AsuC2I	CCSGG	1 cut(s)	829
AsuHPI	GGTGA	4 cut(s)	35, 191, 600, 730
AxyI	CCTNAGG	1 cut(s)	366
BalI	TGGCCA	1 cut(s)	759
BanI	GGYRCC	1 cut(s)	298
BanII	GRGCYC	1 cut(s)	63
BarI	GAAGNNNNNNTAC	2 cut(s)	576, 608
Bbv12I	GWGCWC	1 cut(s)	63
BbvI	GCAGC	5 cut(s)	157, 235, 894, 903, 906
BccI	CCATC	5 cut(s)	310, 749, 952, 998, 1030
BceAI	ACGGC	1 cut(s)	326
BcgI	CGANNNNNNTGC	2 cut(s)	383, 417
BciT130I	CCWGG	1 cut(s)	30
BclI	TGATCA	2 cut(s)	556, 640
BcnI	CCSGG	1 cut(s)	829
BcoDI	GTCTC	2 cut(s)	352, 680
BfaI	CTAG	2 cut(s)	669, 1107
BfmI	CTRYAG	3 cut(s)	48, 817, 1048
BfoI	RGCGCY	1 cut(s)	982
BisI	GCNGC	7 cut(s)	171, 249, 252, 314, 883, 892, 895
BlsI	GCNGC	7 cut(s)	172, 250, 253, 315, 884, 893, 896
BmcAI	AGTACT	1 cut(s)	835
Bme1390I	CCNGG	2 cut(s)	30, 829
BmiI	GGNNCC	2 cut(s)	280, 300
BmrFI	CCNGG	2 cut(s)	30, 829
BmsI	GCATC	1 cut(s)	263
BpuEI	CTTGAG	2 cut(s)	662, 959
BpuMI	CCSGG	1 cut(s)	829
BsaAI	YACGTR	1 cut(s)	1036
BsaBI	GATNNNNATC	1 cut(s)	790
BsaHI	GRCGYC	1 cut(s)	717
BsaI	GGTCTC	1 cut(s)	352
BsaJI	CCNNGG	2 cut(s)	149, 720
BsaXI	ACNNNNNCTCC	4 cut(s)	823, 853, 1025, 1055
Bsc4I	CCNNNNNNNGG	4 cut(s)	258, 368, 632, 969
Bse118I	RCCGGY	2 cut(s)	632, 969
Bse1I	ACTGG	6 cut(s)	280, 389, 660, 741, 781, 841
Bse21I	CCTNAGG	1 cut(s)	366
Bse8I	GATNNNNATC	1 cut(s)	790
BseBI	CCWGG	1 cut(s)	30
BseDI	CCNNGG	2 cut(s)	149, 720
BseGI	GGATG	3 cut(s)	63, 493, 760
BseJI	GATNNNNATC	1 cut(s)	790
BseLI	CCNNNNNNNGG	4 cut(s)	258, 368, 632, 969
BseMII	CTCAG	2 cut(s)	698, 1088
BseNI	ACTGG	6 cut(s)	280, 389, 660, 741, 781, 841
BseXI	GCAGC	5 cut(s)	157, 235, 894, 903, 906
BsgI	GTGCAG	1 cut(s)	79
BshFI	GGCC	4 cut(s)	92, 238, 636, 759
BshNI	GGYRCC	1 cut(s)	298
BsiHKAI	GWGCWC	1 cut(s)	63
BsiSI	CCGG	3 cut(s)	633, 828, 970
BslFI	GGGAC	1 cut(s)	609
BslI	CCNNNNNNNGG	4 cut(s)	258, 368, 632, 969
BsmAI	GTCTC	2 cut(s)	352, 680
BsmFI	GGGAC	1 cut(s)	609
BsmI	GAATGC	1 cut(s)	985
BsnI	GGCC	4 cut(s)	92, 238, 636, 759
Bso31I	GGTCTC	1 cut(s)	352
Bsp1286I	GDGCHC	1 cut(s)	63
Bsp143I	GATC	6 cut(s)	145, 211, 387, 556, 640, 1000
Bsp19I	CCATGG	2 cut(s)	149, 720
BspACI	CCGC	6 cut(s)	252, 313, 752, 854, 860, 1065
BspANI	GGCC	4 cut(s)	92, 238, 636, 759
BspCNI	CTCAG	2 cut(s)	697, 1089
BspHI	TCATGA	1 cut(s)	651
BspLI	GGNNCC	2 cut(s)	280, 300
BspMAI	CTGCAG	1 cut(s)	1052
BspPI	GGATC	2 cut(s)	140, 395
BspQI	GCTCTTC	1 cut(s)	51
BspT107I	GGYRCC	1 cut(s)	298
BspTNI	GGTCTC	1 cut(s)	352
BsrFI	RCCGGY	2 cut(s)	632, 969
BsrI	ACTGG	6 cut(s)	280, 389, 660, 741, 781, 841
BssAI	RCCGGY	2 cut(s)	632, 969
BssECI	CCNNGG	2 cut(s)	149, 720
BssMI	GATC	6 cut(s)	145, 211, 387, 556, 640, 1000
BssNI	GRCGYC	1 cut(s)	717
BssT1I	CCWWGG	2 cut(s)	149, 720
Bst2UI	CCWGG	1 cut(s)	30
Bst4CI	ACNGT	1 cut(s)	932
Bst6I	CTCTTC	1 cut(s)	51
BstACI	GRCGYC	1 cut(s)	717
BstBAI	YACGTR	1 cut(s)	1036
BstC8I	GCNNGC	4 cut(s)	23, 94, 162, 983
BstDEI	CTNAG	3 cut(s)	366, 684, 1097
BstDSI	CCRYGG	2 cut(s)	149, 720
BstF5I	GGATG	3 cut(s)	63, 493, 760
BstH2I	RGCGCY	1 cut(s)	982
BstHHI	GCGC	1 cut(s)	981
BstKTI	GATC	6 cut(s)	148, 214, 390, 559, 643, 1003
BstMAI	GTCTC	2 cut(s)	352, 680
BstMBI	GATC	6 cut(s)	145, 211, 387, 556, 640, 1000
BstMWI	GCNNNNNNNGC	3 cut(s)	170, 888, 891
BstNI	CCWGG	1 cut(s)	30
BstSCI	CCNGG	2 cut(s)	28, 827
BstSFI	CTRYAG	3 cut(s)	48, 817, 1048
BstSNI	TACGTA	1 cut(s)	1036
BstV1I	GCAGC	5 cut(s)	157, 235, 894, 903, 906
BstXI	CCANNNNNNTGG	2 cut(s)	484, 748
Bsu36I	CCTNAGG	1 cut(s)	366
BsuRI	GGCC	4 cut(s)	92, 238, 636, 759
BtgI	CCRYGG	2 cut(s)	149, 720
BtgZI	GCGATG	1 cut(s)	103
BtsCI	GGATG	3 cut(s)	63, 493, 760
BtsI	GCAGTG	2 cut(s)	545, 546
BtsIMutI	CAGTG	2 cut(s)	545, 546
Cac8I	GCNNGC	4 cut(s)	23, 94, 162, 983
CciI	TCATGA	1 cut(s)	651
CfoI	GCGC	1 cut(s)	981
Cfr10I	RCCGGY	2 cut(s)	632, 969
CseI	GACGC	1 cut(s)	725
Csp6I	GTAC	5 cut(s)	193, 220, 657, 825, 834
CviAII	CATG	7 cut(s)	150, 391, 395, 457, 478, 652, 721
CviQI	GTAC	5 cut(s)	193, 220, 657, 825, 834
DdeI	CTNAG	3 cut(s)	366, 684, 1097
DpnI	GATC	6 cut(s)	147, 213, 389, 558, 642, 1002
DpnII	GATC	6 cut(s)	145, 211, 387, 556, 640, 1000
EaeI	YGGCCR	1 cut(s)	757
Eam1104I	CTCTTC	1 cut(s)	51
EarI	CTCTTC	1 cut(s)	51
EciI	GGCGGA	1 cut(s)	767
Ecl136II	GAGCTC	1 cut(s)	61
Eco105I	TACGTA	1 cut(s)	1036
Eco130I	CCWWGG	2 cut(s)	149, 720
Eco24I	GRGCYC	1 cut(s)	63
Eco31I	GGTCTC	1 cut(s)	352
Eco32I	GATATC	2 cut(s)	133, 601
Eco53kI	GAGCTC	1 cut(s)	61
Eco57I	CTGAAG	3 cut(s)	144, 184, 519
Eco81I	CCTNAGG	1 cut(s)	366
EcoICRI	GAGCTC	1 cut(s)	61
EcoRII	CCWGG	1 cut(s)	28
EcoRV	GATATC	2 cut(s)	133, 601
EcoT14I	CCWWGG	2 cut(s)	149, 720
EcoT22I	ATGCAT	1 cut(s)	396
EcoT38I	GRGCYC	1 cut(s)	63
ErhI	CCWWGG	2 cut(s)	149, 720
FaeI	CATG	7 cut(s)	153, 394, 398, 460, 481, 655, 724
FaiI	YATR	8 cut(s)	151, 392, 396, 458, 479, 653, 722, 819
FaqI	GGGAC	1 cut(s)	609
FatI	CATG	7 cut(s)	149, 390, 394, 456, 477, 651, 720
FauI	CCCGC	1 cut(s)	861
FbaI	TGATCA	2 cut(s)	556, 640
Fnu4HI	GCNGC	7 cut(s)	171, 249, 252, 314, 883, 892, 895
FokI	GGATG	3 cut(s)	50, 500, 767
FriOI	GRGCYC	1 cut(s)	63
Fsp4HI	GCNGC	7 cut(s)	171, 249, 252, 314, 883, 892, 895
FspBI	CTAG	2 cut(s)	669, 1107
GlaI	GCGC	1 cut(s)	980
GluI	GCNGC	7 cut(s)	171, 249, 252, 314, 883, 892, 895
HaeII	RGCGCY	1 cut(s)	982
HaeIII	GGCC	4 cut(s)	92, 238, 636, 759
HapII	CCGG	3 cut(s)	633, 828, 970
HgaI	GACGC	1 cut(s)	725
HhaI	GCGC	1 cut(s)	981
Hin1I	GRCGYC	1 cut(s)	717
Hin1II	CATG	7 cut(s)	153, 394, 398, 460, 481, 655, 724
Hin6I	GCGC	1 cut(s)	979
HinP1I	GCGC	1 cut(s)	979
HindIII	AAGCTT	1 cut(s)	613
HinfI	GANTC	4 cut(s)	14, 372, 507, 1081
HpaII	CCGG	3 cut(s)	633, 828, 970
HphI	GGTGA	4 cut(s)	35, 191, 600, 730
Hpy166II	GTNNAC	1 cut(s)	1039
Hpy188I	TCNGA	2 cut(s)	687, 1098
Hpy188III	TCNNGA	7 cut(s)	306, 376, 410, 575, 652, 1078, 1121
Hpy8I	GTNNAC	1 cut(s)	1039
HpyAV	CCTTC	6 cut(s)	77, 79, 262, 328, 416, 709
HpyCH4III	ACNGT	1 cut(s)	932
HpyCH4IV	ACGT	2 cut(s)	321, 1035
HpyCH4V	TGCA	3 cut(s)	96, 394, 1050
HpyF10VI	GCNNNNNNNGC	3 cut(s)	170, 888, 891
HpyF3I	CTNAG	3 cut(s)	366, 684, 1097
HpySE526I	ACGT	2 cut(s)	321, 1035
Hsp92I	GRCGYC	1 cut(s)	717
Hsp92II	CATG	7 cut(s)	153, 394, 398, 460, 481, 655, 724
HspAI	GCGC	1 cut(s)	979
Ksp22I	TGATCA	2 cut(s)	556, 640
Kzo9I	GATC	6 cut(s)	145, 211, 387, 556, 640, 1000
LguI	GCTCTTC	1 cut(s)	51
LmnI	GCTCC	1 cut(s)	958
Lsp1109I	GCAGC	5 cut(s)	157, 235, 894, 903, 906
LweI	GCATC	1 cut(s)	263
MaeI	CTAG	2 cut(s)	669, 1107
MaeII	ACGT	2 cut(s)	321, 1035
MaeIII	GTNAC	1 cut(s)	529
MalI	GATC	6 cut(s)	147, 213, 389, 558, 642, 1002
MboI	GATC	6 cut(s)	145, 211, 387, 556, 640, 1000
MboII	GAAGA	7 cut(s)	68, 190, 343, 590, 593, 596, 1039
MfeI	CAATTG	1 cut(s)	155
MhlI	GDGCHC	1 cut(s)	63
MlsI	TGGCCA	1 cut(s)	759
MluCI	AATT	4 cut(s)	155, 780, 1069, 1088
MluNI	TGGCCA	1 cut(s)	759
MmeI	TCCRAC	3 cut(s)	465, 729, 1068
MnlI	CCTC	4 cut(s)	228, 474, 693, 930
Mox20I	TGGCCA	1 cut(s)	759
Mph1103I	ATGCAT	1 cut(s)	396
MscI	TGGCCA	1 cut(s)	759
Msp20I	TGGCCA	1 cut(s)	759
MspA1I	CMGCKG	1 cut(s)	862
MspI	CCGG	3 cut(s)	633, 828, 970
MspR9I	CCNGG	2 cut(s)	30, 829
MunI	CAATTG	1 cut(s)	155
Mva1269I	GAATGC	1 cut(s)	985
MvaI	CCWGG	1 cut(s)	30
MwoI	GCNNNNNNNGC	3 cut(s)	170, 888, 891
NciI	CCSGG	1 cut(s)	829
NcoI	CCATGG	2 cut(s)	149, 720
NdeII	GATC	6 cut(s)	145, 211, 387, 556, 640, 1000
NlaIII	CATG	7 cut(s)	153, 394, 398, 460, 481, 655, 724
NlaIV	GGNNCC	2 cut(s)	280, 300
NmuCI	GTSAC	1 cut(s)	529
NsiI	ATGCAT	1 cut(s)	396
PagI	TCATGA	1 cut(s)	651
PciSI	GCTCTTC	1 cut(s)	51
PctI	GAATGC	1 cut(s)	985
PfeI	GAWTC	4 cut(s)	14, 372, 507, 1081
PkrI	GCNGC	7 cut(s)	172, 250, 253, 315, 884, 893, 896
Ppu21I	YACGTR	1 cut(s)	1036
Psp124BI	GAGCTC	1 cut(s)	63
Psp1406I	AACGTT	1 cut(s)	321
Psp6I	CCWGG	1 cut(s)	28
PspGI	CCWGG	1 cut(s)	28
PspN4I	GGNNCC	2 cut(s)	280, 300
PstI	CTGCAG	1 cut(s)	1052
RsaI	GTAC	5 cut(s)	194, 221, 658, 826, 835
RsaNI	GTAC	5 cut(s)	193, 220, 657, 825, 834
SacI	GAGCTC	1 cut(s)	63
SapI	GCTCTTC	1 cut(s)	51
SatI	GCNGC	7 cut(s)	171, 249, 252, 314, 883, 892, 895
Sau3AI	GATC	6 cut(s)	145, 211, 387, 556, 640, 1000
ScaI	AGTACT	1 cut(s)	835
ScrFI	CCNGG	2 cut(s)	30, 829
SduI	GDGCHC	1 cut(s)	63
SfaNI	GCATC	1 cut(s)	263
SfcI	CTRYAG	3 cut(s)	48, 817, 1048
SmlI	CTYRAG	2 cut(s)	677, 974
SmoI	CTYRAG	2 cut(s)	677, 974
SnaBI	TACGTA	1 cut(s)	1036
Sse9I	AATT	4 cut(s)	155, 780, 1069, 1088
SsiI	CCGC	6 cut(s)	252, 313, 752, 854, 860, 1065
SspMI	CTAG	2 cut(s)	669, 1107
SstI	GAGCTC	1 cut(s)	63
StyD4I	CCNGG	2 cut(s)	28, 827
StyI	CCWWGG	2 cut(s)	149, 720
TaaI	ACNGT	1 cut(s)	932
TaiI	ACGT	2 cut(s)	324, 1038
TaqI	TCGA	3 cut(s)	505, 521, 1025
TasI	AATT	4 cut(s)	155, 780, 1069, 1088
TatI	WGTACW	1 cut(s)	833
TauI	GCSGC	2 cut(s)	254, 316
TfiI	GAWTC	4 cut(s)	14, 372, 507, 1081
TscAI	CASTG	2 cut(s)	546, 552
TseFI	GTSAC	1 cut(s)	529
TseI	GCWGC	5 cut(s)	170, 248, 882, 891, 894
Tsp45I	GTSAC	1 cut(s)	529
TspDTI	ATGAA	5 cut(s)	17, 69, 473, 1062, 1103
TspGWI	ACGGA	1 cut(s)	103
TspRI	CASTG	2 cut(s)	546, 552
XspI	CTAG	2 cut(s)	669, 1107
ZrmI	AGTACT	1 cut(s)	835
Zsp2I	ATGCAT	1 cut(s)	396

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.