Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0015962

Physical Location & Seq

Genomic Coordinates Reverse (-)

942233 .. 946341

Gene Structure 4109 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1008900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1572 bp

ATGAACATGGAGTCCTTAGCTGTATTCAACAACTCATTCTCACTCAGCTCTCCCTCACATGACCACTTCATCCCAAAAAACCTTCGACCCTTTCGTATGGCGTCTTTGCAATCTCACAGACAAATGCCCAAGCTGGAGGTCCGATGTGCCACCCTGGCCCAATCCACTGACCCCAAGTCCAAGGAGGCCAAGCTCTGGGGTGGTCGCTTTGAGGAGAGTGTGACTGACGCTGTCGAGCGATTTACTGAGTCCATCTCGTTTGACAAAGCCCTCTACAAGCATGATATCATGGGCAGCAAGGCTCACGCTTCCATGCTAGCCAAACAGGGATTGATTACTGTTAATGACCGGGATAGCATCCACAAGGGGCTGAGCGAGATAGAAAGGCGAATTGAGAGTGGTGAGTTTGAATGGAGGACCGACAGAGAAGATGTGCACATGAACATTGAGGCAGCGCTGACTGATTTGATCGGCGAACCGGCTAAGAAGCTCCACACGGCTCGGAGCCGAAACGATCAGGTTTTGACAGATTTCCGATTGTGGATCCGAGACGCGATTGATGCAATTGGTGTGAAGATTAAGTATCTTCAAGTTTCGCTCGTCAAATTGGCAATGAAGAACGAGGGTGTGATTGTTCCTGGTTACACGCATCTGCAAAGGGCTCAACCTGTTCTGCTTCAGCATTTGCTCTTGGCATTTGTTGAGCAGCTTGAACGTGATGCTGGCCGTTTACTGGATTGCAGAGTGAGGTTGAATTTCTGCCCTTTAGGCGCTTGTGCATTGGCCGGCACAGGACTTCCAATTGATCGGTTTATGACCTCTGATGCTTTAGAATTCACTGCTCCTATGAGGAACAGTATTGATGCAGTGTCAGACCGAGATTTTGTCATGGAGTTTCTTTCTGCTAACGCCATCACAGCAATTCATCTCTCCCGGCTTGGTGAAGAATGGGTACTGTGGGCTTCAGAGGAGTTTGGATTTATCACGCCAAGTGACAAGGTTTCAACAGGAAGTAGTATCATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTTGTTCGTGGAAAGTCCGCTAGAGTAGTAGGAGACCTGGTTACCCTTCTCACATTGTGCAAGGGACTTCCCCTTGCTTACAATCGTGATTTACAGGAAGATAAGGAGCCTGTCTTCGACAGCGTGAAGACAATATTAGGGATGCTTGAAGTGTCTGCAGAGTTTGCACAGAACATTACCTTTAACAGGGAGAGAATACAAAAGAGTTTACCTGCTGGTCACCTTGATGCCACAACGCTTGCCGATTATCTCGTGAAAAAGGGAATACCTTTCAGAACCTCTCATGACATAGTTGGAAGGGCAGTTGCCGTGTGCGTCTCAAAAAGTTGCGAACTGCAGGCATTGAGTGTCGATGAGCTCAAAAGTATCGATCCAGTGTTTGAAAAAGATGTATACGAATTTCTCGGAGTAGAGAATGCAGTGAAGAAATTCTCCTCGTATGGTTCCACAGGGTCAGCATGCGTCGCCAGTCAGCTCGATCATTGGGTTACAAAACTTGAAATCAACAAAGGGGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004056 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006591 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016829 GO:0016840 GO:0016842 GO:0019752 GO:0042450 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00220 ko00250 ko01100 ko01110 ko01130 ko01230 map00220 map00250 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

ASL_C2 Lyase_1

Protein Analysis

524

Amino Acids

58.34

Weight (kDa)

6.2

Isoelectric Point (pI)

37.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lyase_1	PF00206	68 - 362	1.8e-69	Lyase
ASL_C2	PF14698	426 - 493	8.4e-26	Argininosuccinate lyase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015962)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10920
fragaria_vesca	FvH4_5g34710
malus_domestica	MD12G1008900.v1.1 MD14G1006400.v1.1
prunus_persica	Prupe.1G532200_v2.0.a1
pyrus_communis	pycom12g00670 pycom14g00490
rosa_chinensis	RchiOBHm_Chr7g0216141
rosa_laevigata	RLG00000002592
rosa_multiflora	Rmu_sc0021916.1_g000001
rosa_roxburghii	Rroxscaffold_3G00243610
rosa_rugosa	Rorug07G0159000
rosa_samantha	Rh7AG296200 Rh7BG288500
rosa_wichuraiana	Rw7G025200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1273
AccB7I	CCANNNNNTGG	1 cut(s)	195
AccI	GTMKAC	1 cut(s)	1446
AccII	CGCG	1 cut(s)	554
AciI	CCGC	1 cut(s)	1071
AclWI	GGATC	4 cut(s)	538, 551, 1037, 1418
AcoI	YGGCCR	2 cut(s)	724, 783
AcsI	RAATTY	4 cut(s)	754, 833, 1451, 1481
AcuI	CTGAAG	2 cut(s)	662, 948
AcyI	GRCGYC	1 cut(s)	101
AdeI	CACNNNGTG	1 cut(s)	1110
AfaI	GTAC	1 cut(s)	954
AfeI	AGCGCT	1 cut(s)	456
AfiI	CCNNNNNNNGG	3 cut(s)	195, 733, 1239
AgsI	TTSAA	9 cut(s)	28, 410, 590, 713, 754, 1005, 1202, 1436, 1553
AhdI	GACNNNNNGTC	1 cut(s)	175
AjnI	CCWGG	3 cut(s)	153, 637, 1089
AloI	GAACNNNNNNTCC	1 cut(s)	28
AluBI	AGCT	8 cut(s)	20, 48, 133, 193, 490, 709, 1411, 1528
AluI	AGCT	8 cut(s)	20, 48, 133, 193, 490, 709, 1411, 1528
Alw21I	GWGCWC	2 cut(s)	438, 1413
Alw26I	GTCTC	3 cut(s)	543, 1080, 1375
Alw44I	GTGCAC	1 cut(s)	434
AlwI	GGATC	4 cut(s)	538, 551, 1037, 1418
Aor51HI	AGCGCT	1 cut(s)	456
AoxI	GGCC	4 cut(s)	156, 186, 724, 783
ApaLI	GTGCAC	1 cut(s)	434
ApeKI	GCWGC	3 cut(s)	294, 452, 706
ApoI	RAATTY	4 cut(s)	754, 833, 1451, 1481
ArsI	GACNNNNNNTTYG	2 cut(s)	866, 898
Asp700I	GAANNNNTTC	2 cut(s)	1056, 1481
AspLEI	GCGC	2 cut(s)	457, 773
AspS9I	GGNCC	3 cut(s)	139, 157, 417
AsuC2I	CCSGG	2 cut(s)	350, 934
AsuHPI	GGTGA	3 cut(s)	413, 953, 1265
AsuNHI	GCTAGC	1 cut(s)	316
AvaII	GGWCC	2 cut(s)	139, 417
BaeGI	GKGCMC	1 cut(s)	438
BamHI	GGATCC	1 cut(s)	543
BanII	GRGCYC	2 cut(s)	664, 1413
BarI	GAAGNNNNNNTAC	2 cut(s)	936, 968
BauI	CACGAG	1 cut(s)	1304
BbsI	GAAGAC	2 cut(s)	1159, 1187
Bbv12I	GWGCWC	2 cut(s)	438, 1413
BbvI	GCAGC	3 cut(s)	306, 464, 718
BccI	CCATC	2 cut(s)	260, 920
BceAI	ACGGC	3 cut(s)	513, 711, 1346
BciT130I	CCWGG	3 cut(s)	155, 639, 1091
BcnI	CCSGG	2 cut(s)	350, 934
BcoDI	GTCTC	3 cut(s)	543, 1080, 1375
BfaI	CTAG	2 cut(s)	317, 1074
BfmI	CTRYAG	2 cut(s)	1209, 1388
BfoI	RGCGCY	2 cut(s)	458, 774
BfuAI	ACCTGC	1 cut(s)	1273
BglI	GCCNNNNNGGC	2 cut(s)	155, 768
BisI	GCNGC	3 cut(s)	295, 453, 707
BlpI	GCTNAGC	1 cut(s)	371
BlsI	GCNGC	3 cut(s)	296, 454, 708
Bme1390I	CCNGG	5 cut(s)	155, 350, 639, 934, 1091
Bme18I	GGWCC	2 cut(s)	139, 417
BmeRI	GACNNNNNGTC	1 cut(s)	175
BmgT120I	GGNCC	3 cut(s)	139, 157, 417
BmiI	GGNNCC	4 cut(s)	506, 545, 1161, 1498
BmrFI	CCNGG	5 cut(s)	155, 350, 639, 934, 1091
BmsI	GCATC	8 cut(s)	366, 550, 658, 709, 814, 853, 1185, 1270
BmtI	GCTAGC	1 cut(s)	320
BpiI	GAAGAC	2 cut(s)	1159, 1187
BplI	GAGNNNNNCTC	2 cut(s)	239, 271
BpmI	CTGGAG	1 cut(s)	155
Bpu10I	CCTNAGC	1 cut(s)	16
Bpu1102I	GCTNAGC	1 cut(s)	371
BpuMI	CCSGG	2 cut(s)	350, 934
Bsa29I	ATCGAT	1 cut(s)	1422
BsaHI	GRCGYC	1 cut(s)	101
BsaI	GGTCTC	1 cut(s)	1080
BsaJI	CCNNGG	2 cut(s)	153, 180
BsaXI	ACNNNNNCTCC	2 cut(s)	1077, 1107
Bsc4I	CCNNNNNNNGG	3 cut(s)	195, 733, 1239
Bse118I	RCCGGY	2 cut(s)	478, 785
Bse1I	ACTGG	3 cut(s)	738, 1427, 1521
Bse3DI	GCAATG	1 cut(s)	618
BseBI	CCWGG	3 cut(s)	155, 639, 1091
BseCI	ATCGAT	1 cut(s)	1422
BseDI	CCNNGG	2 cut(s)	153, 180
BseGI	GGATG	3 cut(s)	69, 357, 1200
BseLI	CCNNNNNNNGG	3 cut(s)	195, 733, 1239
BseMI	GCAATG	1 cut(s)	618
BseMII	CTCAG	4 cut(s)	58, 237, 362, 1040
BseNI	ACTGG	3 cut(s)	738, 1427, 1521
BseRI	GAGGAG	3 cut(s)	227, 983, 1477
BseSI	GKGCMC	1 cut(s)	438
BseXI	GCAGC	3 cut(s)	306, 464, 718
Bsh1236I	CGCG	1 cut(s)	554
BshFI	GGCC	4 cut(s)	158, 188, 726, 785
BshVI	ATCGAT	1 cut(s)	1422
BsiHKAI	GWGCWC	2 cut(s)	438, 1413
BsiSI	CCGG	4 cut(s)	349, 479, 786, 934
BslFI	GGGAC	1 cut(s)	1131
BslI	CCNNNNNNNGG	3 cut(s)	195, 733, 1239
BsmAI	GTCTC	3 cut(s)	543, 1080, 1375
BsmBI	CGTCTC	2 cut(s)	543, 1375
BsmFI	GGGAC	1 cut(s)	1131
BsmI	GAATGC	1 cut(s)	1474
BsnI	GGCC	4 cut(s)	158, 188, 726, 785
Bso31I	GGTCTC	1 cut(s)	1080
Bsp1286I	GDGCHC	3 cut(s)	438, 664, 1413
Bsp143I	GATC	7 cut(s)	468, 514, 543, 805, 1042, 1423, 1531
Bsp1720I	GCTNAGC	1 cut(s)	371
BspACI	CCGC	1 cut(s)	1071
BspANI	GGCC	4 cut(s)	158, 188, 726, 785
BspCNI	CTCAG	4 cut(s)	57, 238, 363, 1039
BspDI	ATCGAT	1 cut(s)	1422
BspFNI	CGCG	1 cut(s)	554
BspHI	TCATGA	1 cut(s)	1336
BspLI	GGNNCC	4 cut(s)	506, 545, 1161, 1498
BspMAI	CTGCAG	2 cut(s)	1213, 1392
BspMI	ACCTGC	1 cut(s)	1273
BspOI	GCTAGC	1 cut(s)	320
BspPI	GGATC	4 cut(s)	538, 551, 1037, 1418
BspTNI	GGTCTC	1 cut(s)	1080
BsrDI	GCAATG	1 cut(s)	618
BsrFI	RCCGGY	2 cut(s)	478, 785
BsrI	ACTGG	3 cut(s)	738, 1427, 1521
BssAI	RCCGGY	2 cut(s)	478, 785
BssECI	CCNNGG	2 cut(s)	153, 180
BssMI	GATC	7 cut(s)	468, 514, 543, 805, 1042, 1423, 1531
BssNAI	GTATAC	1 cut(s)	1447
BssNI	GRCGYC	1 cut(s)	101
BssSI	CACGAG	1 cut(s)	1304
BssT1I	CCWWGG	1 cut(s)	180
Bst1107I	GTATAC	1 cut(s)	1447
Bst2BI	CACGAG	1 cut(s)	1304
Bst2UI	CCWGG	3 cut(s)	155, 639, 1091
Bst4CI	ACNGT	3 cut(s)	340, 857, 957
BstACI	GRCGYC	1 cut(s)	101
BstAPI	GCANNNNNTGC	1 cut(s)	1217
BstC8I	GCNNGC	6 cut(s)	318, 724, 787, 1293, 1392, 1513
BstDEI	CTNAG	6 cut(s)	16, 44, 246, 371, 483, 1026
BstEII	GGTNACC	2 cut(s)	1093, 1271
BstENI	CCTNNNNNAGG	1 cut(s)	1237
BstF5I	GGATG	3 cut(s)	69, 357, 1200
BstFNI	CGCG	1 cut(s)	554
BstH2I	RGCGCY	2 cut(s)	458, 774
BstHHI	GCGC	2 cut(s)	457, 773
BstKTI	GATC	7 cut(s)	471, 517, 546, 808, 1045, 1426, 1534
BstMAI	GTCTC	3 cut(s)	543, 1080, 1375
BstMBI	GATC	7 cut(s)	468, 514, 543, 805, 1042, 1423, 1531
BstMWI	GCNNNNNNNGC	6 cut(s)	155, 560, 768, 917, 1217, 1517
BstNI	CCWGG	3 cut(s)	155, 639, 1091
BstNSI	RCATGY	1 cut(s)	1515
BstPI	GGTNACC	2 cut(s)	1093, 1271
BstSCI	CCNGG	5 cut(s)	153, 348, 637, 932, 1089
BstSFI	CTRYAG	2 cut(s)	1209, 1388
BstSLI	GKGCMC	1 cut(s)	438
BstUI	CGCG	1 cut(s)	554
BstV1I	GCAGC	3 cut(s)	306, 464, 718
BstV2I	GAAGAC	2 cut(s)	1159, 1187
BstX2I	RGATCY	2 cut(s)	543, 1042
BstYI	RGATCY	2 cut(s)	543, 1042
BstZ17I	GTATAC	1 cut(s)	1447
Bsu15I	ATCGAT	1 cut(s)	1422
BsuRI	GGCC	4 cut(s)	158, 188, 726, 785
BsuTUI	ATCGAT	1 cut(s)	1422
BtsCI	GGATG	3 cut(s)	69, 357, 1200
BtsI	GCAGTG	3 cut(s)	837, 873, 1479
BtsIMutI	CAGTG	5 cut(s)	165, 837, 873, 1434, 1479
BveI	ACCTGC	1 cut(s)	1273
Cac8I	GCNNGC	6 cut(s)	318, 724, 787, 1293, 1392, 1513
CciI	TCATGA	1 cut(s)	1336
CfoI	GCGC	2 cut(s)	457, 773
Cfr10I	RCCGGY	2 cut(s)	478, 785
Cfr13I	GGNCC	3 cut(s)	139, 157, 417
ClaI	ATCGAT	1 cut(s)	1422
CseI	GACGC	5 cut(s)	90, 236, 560, 1357, 1504
CsiI	ACCWGGT	1 cut(s)	1089
Csp6I	GTAC	1 cut(s)	953
CspCI	CAANNNNNGTGG	2 cut(s)	1273, 1308
CviQI	GTAC	1 cut(s)	953
DdeI	CTNAG	6 cut(s)	16, 44, 246, 371, 483, 1026
DpnI	GATC	7 cut(s)	470, 516, 545, 807, 1044, 1425, 1533
DpnII	GATC	7 cut(s)	468, 514, 543, 805, 1042, 1423, 1531
DraIII	CACNNNGTG	1 cut(s)	1110
DriI	GACNNNNNGTC	1 cut(s)	175
EaeI	YGGCCR	2 cut(s)	724, 783
Eam1105I	GACNNNNNGTC	1 cut(s)	175
Ecl136II	GAGCTC	1 cut(s)	1411
Eco130I	CCWWGG	1 cut(s)	180
Eco24I	GRGCYC	2 cut(s)	664, 1413
Eco31I	GGTCTC	1 cut(s)	1080
Eco32I	GATATC	1 cut(s)	286
Eco47I	GGWCC	2 cut(s)	139, 417
Eco47III	AGCGCT	1 cut(s)	456
Eco53kI	GAGCTC	1 cut(s)	1411
Eco57I	CTGAAG	2 cut(s)	662, 948
Eco91I	GGTNACC	2 cut(s)	1093, 1271
EcoICRI	GAGCTC	1 cut(s)	1411
EcoNI	CCTNNNNNAGG	1 cut(s)	1237
EcoO65I	GGTNACC	2 cut(s)	1093, 1271
EcoRI	GAATTC	1 cut(s)	833
EcoRII	CCWGG	3 cut(s)	153, 637, 1089
EcoRV	GATATC	1 cut(s)	286
EcoT14I	CCWWGG	1 cut(s)	180
EcoT38I	GRGCYC	2 cut(s)	664, 1413
ErhI	CCWWGG	1 cut(s)	180
Esp3I	CGTCTC	2 cut(s)	543, 1375
FaqI	GGGAC	1 cut(s)	1131
FblI	GTMKAC	1 cut(s)	1446
Fnu4HI	GCNGC	3 cut(s)	295, 453, 707
FokI	GGATG	3 cut(s)	56, 344, 1207
FriOI	GRGCYC	2 cut(s)	664, 1413
Fsp4HI	GCNGC	3 cut(s)	295, 453, 707
FspBI	CTAG	2 cut(s)	317, 1074
GlaI	GCGC	2 cut(s)	456, 772
GluI	GCNGC	3 cut(s)	295, 453, 707
GsuI	CTGGAG	1 cut(s)	155
HaeII	RGCGCY	2 cut(s)	458, 774
HaeIII	GGCC	4 cut(s)	158, 188, 726, 785
HapII	CCGG	4 cut(s)	349, 479, 786, 934
HgaI	GACGC	5 cut(s)	90, 236, 560, 1357, 1504
HhaI	GCGC	2 cut(s)	457, 773
Hin1I	GRCGYC	1 cut(s)	101
Hin6I	GCGC	2 cut(s)	455, 771
HinP1I	GCGC	2 cut(s)	455, 771
HinfI	GANTC	2 cut(s)	11, 248
HpaII	CCGG	4 cut(s)	349, 479, 786, 934
HphI	GGTGA	3 cut(s)	413, 953, 1265
Hpy166II	GTNNAC	4 cut(s)	436, 731, 1262, 1447
Hpy188III	TCNNGA	4 cut(s)	1040, 1139, 1306, 1337
Hpy8I	GTNNAC	4 cut(s)	436, 731, 1262, 1447
Hpy99I	CGWCG	1 cut(s)	1520
HpyAV	CCTTC	3 cut(s)	92, 1109, 1344
HpyCH4III	ACNGT	3 cut(s)	340, 857, 957
HpyCH4IV	ACGT	1 cut(s)	715
HpyF10VI	GCNNNNNNNGC	6 cut(s)	155, 560, 768, 917, 1217, 1517
HpyF3I	CTNAG	6 cut(s)	16, 44, 246, 371, 483, 1026
HpySE526I	ACGT	1 cut(s)	715
Hsp92I	GRCGYC	1 cut(s)	101
HspAI	GCGC	2 cut(s)	455, 771
KroI	GCCGGC	1 cut(s)	785
KroNI	GCCGGC	1 cut(s)	787
Kzo9I	GATC	7 cut(s)	468, 514, 543, 805, 1042, 1423, 1531
LmnI	GCTCC	4 cut(s)	495, 504, 847, 1159
Lsp1109I	GCAGC	3 cut(s)	306, 464, 718
LweI	GCATC	8 cut(s)	366, 550, 658, 709, 814, 853, 1185, 1270
MabI	ACCWGGT	1 cut(s)	1089
MaeI	CTAG	2 cut(s)	317, 1074
MaeII	ACGT	1 cut(s)	715
MaeIII	GTNAC	6 cut(s)	220, 641, 992, 1093, 1271, 1540
MalI	GATC	7 cut(s)	470, 516, 545, 807, 1044, 1425, 1533
MboI	GATC	7 cut(s)	468, 514, 543, 805, 1042, 1423, 1531
MfeI	CAATTG	2 cut(s)	564, 801
MflI	RGATCY	2 cut(s)	543, 1042
MhlI	GDGCHC	3 cut(s)	438, 664, 1413
MluCI	AATT	9 cut(s)	390, 564, 605, 754, 801, 833, 921, 1451, 1481
MlyI	GAGTC	2 cut(s)	20, 257
MmeI	TCCRAC	1 cut(s)	1327
MroNI	GCCGGC	1 cut(s)	785
MroXI	GAANNNNTTC	2 cut(s)	1056, 1481
MseI	TTAA	3 cut(s)	342, 579, 1236
MslI	CAYNNNNRTG	1 cut(s)	1278
MspI	CCGG	4 cut(s)	349, 479, 786, 934
MspR9I	CCNGG	5 cut(s)	155, 350, 639, 934, 1091
MunI	CAATTG	2 cut(s)	564, 801
Mva1269I	GAATGC	1 cut(s)	1474
MvaI	CCWGG	3 cut(s)	155, 639, 1091
MvnI	CGCG	1 cut(s)	554
MwoI	GCNNNNNNNGC	6 cut(s)	155, 560, 768, 917, 1217, 1517
NaeI	GCCGGC	1 cut(s)	787
NciI	CCSGG	2 cut(s)	350, 934
NdeII	GATC	7 cut(s)	468, 514, 543, 805, 1042, 1423, 1531
NgoMIV	GCCGGC	1 cut(s)	785
NheI	GCTAGC	1 cut(s)	316
NlaIV	GGNNCC	4 cut(s)	506, 545, 1161, 1498
NmuCI	GTSAC	3 cut(s)	220, 992, 1271
NspI	RCATGY	1 cut(s)	1515
PaeI	GCATGC	1 cut(s)	1515
PagI	TCATGA	1 cut(s)	1336
PcsI	WCGNNNNNNNCGW	1 cut(s)	91
PctI	GAATGC	1 cut(s)	1474
PdiI	GCCGGC	1 cut(s)	787
PdmI	GAANNNNTTC	2 cut(s)	1056, 1481
PflFI	GACNNNGTC	1 cut(s)	230
PflMI	CCANNNNNTGG	1 cut(s)	195
PkrI	GCNGC	3 cut(s)	296, 454, 708
PleI	GAGTC	2 cut(s)	19, 256
PpsI	GAGTC	2 cut(s)	19, 256
Psp124BI	GAGCTC	1 cut(s)	1413
Psp6I	CCWGG	3 cut(s)	153, 637, 1089
PspEI	GGTNACC	2 cut(s)	1093, 1271
PspGI	CCWGG	3 cut(s)	153, 637, 1089
PspN4I	GGNNCC	4 cut(s)	506, 545, 1161, 1498
PspPI	GGNCC	3 cut(s)	139, 157, 417
PstI	CTGCAG	2 cut(s)	1213, 1392
PsuI	RGATCY	2 cut(s)	543, 1042
PsyI	GACNNNGTC	1 cut(s)	230
RsaI	GTAC	1 cut(s)	954
RsaNI	GTAC	1 cut(s)	953
RseI	CAYNNNNRTG	1 cut(s)	1278
SacI	GAGCTC	1 cut(s)	1413
SaqAI	TTAA	3 cut(s)	342, 579, 1236
SatI	GCNGC	3 cut(s)	295, 453, 707
Sau3AI	GATC	7 cut(s)	468, 514, 543, 805, 1042, 1423, 1531
Sau96I	GGNCC	3 cut(s)	139, 157, 417
SchI	GAGTC	2 cut(s)	20, 257
ScrFI	CCNGG	5 cut(s)	155, 350, 639, 934, 1091
SduI	GDGCHC	3 cut(s)	438, 664, 1413
SexAI	ACCWGGT	1 cut(s)	1089
SfaNI	GCATC	8 cut(s)	366, 550, 658, 709, 814, 853, 1185, 1270
SfcI	CTRYAG	2 cut(s)	1209, 1388
SinI	GGWCC	2 cut(s)	139, 417
SmiMI	CAYNNNNRTG	1 cut(s)	1278
SphI	GCATGC	1 cut(s)	1515
Sse9I	AATT	9 cut(s)	390, 564, 605, 754, 801, 833, 921, 1451, 1481
SsiI	CCGC	1 cut(s)	1071
SspI	AATATT	1 cut(s)	1188
SspMI	CTAG	2 cut(s)	317, 1074
SstI	GAGCTC	1 cut(s)	1413
StyD4I	CCNGG	5 cut(s)	153, 348, 637, 932, 1089
StyI	CCWWGG	1 cut(s)	180
TaaI	ACNGT	3 cut(s)	340, 857, 957
TaiI	ACGT	1 cut(s)	718
TaqI	TCGA	6 cut(s)	85, 234, 1170, 1404, 1422, 1530
TaqII	GACCGA	2 cut(s)	434, 891
TasI	AATT	9 cut(s)	390, 564, 605, 754, 801, 833, 921, 1451, 1481
Tru1I	TTAA	3 cut(s)	342, 579, 1236
Tru9I	TTAA	3 cut(s)	342, 579, 1236
TscAI	CASTG	5 cut(s)	172, 844, 873, 1434, 1479
TseFI	GTSAC	3 cut(s)	220, 992, 1271
TseI	GCWGC	3 cut(s)	294, 452, 706
Tsp45I	GTSAC	3 cut(s)	220, 992, 1271
TspDTI	ATGAA	5 cut(s)	17, 58, 455, 629, 914
TspRI	CASTG	5 cut(s)	172, 844, 873, 1434, 1479
Tth111I	GACNNNGTC	1 cut(s)	230
Van91I	CCANNNNNTGG	1 cut(s)	195
VneI	GTGCAC	1 cut(s)	434
VpaK11BI	GGWCC	2 cut(s)	139, 417
XagI	CCTNNNNNAGG	1 cut(s)	1237
XapI	RAATTY	4 cut(s)	754, 833, 1451, 1481
XceI	RCATGY	1 cut(s)	1515
XmiI	GTMKAC	1 cut(s)	1446
XmnI	GAANNNNTTC	2 cut(s)	1056, 1481
XspI	CTAG	2 cut(s)	317, 1074

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.