MD12G1022300.v1.1
BZIP Family

basic region leucin zipper

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008817
Physical Location & Seq
Forward (+)
2218624 .. 2222646
4023 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1022300.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGGTTTCTTCCGAGGGGCGGTCGTTTTCGAGCTCGACGGGGTCCACGGCGGCGGGGTCATCGTCGTCGCCCGACTCCGGTTCTTATTCGTCGTCGACGAACGGTGCGGTCGAGAGATTTGGTGGAAGCGGCGGCGGTGTGGATCGGATGGTGAAAGAGGAGCTCGAGGCGGCGGAGGCGCTTGCGGATTTGGCGCATTTGGCGATGCGCGAGAGTAGCGGCGCCGAATCCGCTGGGAATTGGGGGCTGAAAAGGAAGCGAGCCGGGAAGCGAGTCAAGAGTGGGTCTCCGCCGAGTCAGTCGGGTTTGTACCCGCCTGACCCGGCTCCCACATGTCCGGATCTTCCTCAGGATCAAGCGGTGACAGGTTTGCGGCAATGTGAAACGGTATGCACAAATGTCGTTACAGAACTGGTGAAGACTGAGCAAGTTCTGAGTGAAAAGGTTGTGAGGGCTGAGCATGATCCAGAATTAAATAAGCCAAGTCCTATATGCACTACAAGTCACCCTTCATTTAGTTGCAGCAAGTCAAGGCGGAATTTAACTGAGGAGGAAAAGGAAGAGCGGAGAATACGCAGAGTATTGGCAAATCGAGAGTCAGCTAGACAAACAATTCGTCGACGTCAAGCTCTATGTGAGGAGTTAACCAGAAAAGCTGCCGATCTGGCATTGGAGAATGAAAATCTGAAAAGGAAAAAGGAGCTAGCGGTAAAGGAGTTCCAGTCTTTGGAGAAAACAAATAAGCACTTGAAAGTTCAGATGACTAAGGTGATAAGGGCAGAGGATGAGAAAACAGCAGGTGAAAATATGTCAGCCTATGTGCAGATGCAGATACCTCCGTCTTCATCTACAAATTCCCCGTTCTTCTTGTTTAACCGTCCCCCATTTGCACCACTTTTCTGGCCTTCTATCATCCAGGCTTCAAACTCTACTCAGGTCCAGCACATACCACAAAATCCAATAGCCATTCCGTCAAACATCTCATTGCCGGCTAATGGTGCAGCTGACTCTTCTCTTGAACAAAACCCCTCAAACATCAATGGAGCGAGAGCTCCTTTATATGTGTTTCCGTGTCCTTGGTTCATCCCTCACTTTGATAATGGGAATGGACTCCAACCCCAGTCTTCCTTGTGTTTGAACAATAAGCAAGAAGAAACTTCTTTTAATAACCAGTTCAGTGCTAATTCATCTTCAAGAACCGTTGCGCAGTTGGACAACCACCAGTCCTCTTTCCCTGTTAGATTAAAAACAGAAGATTCCCCTTCAATTGAAGGCAGACCGTCATGTGATCTTAACGAGACCCCTGCTCAGTTTCCTCTGGATGGAGGTGATCAGCACACAGGACCTCACCCGATGGAAAATGGTTGCAAGGAAATATTCCCTTCTGCGGCATCACTCAACCATTCCGGAGTTGCATCTTGTATCAAGAATGAGAACGGATTTGAATCAGATTACACTGTAACAACTCTGAACTCTTATCACAAGTTCTCTGCCTTGCCAGACAAGAATAGTGAACCAATCATCTACCCTACTAGGAAACTCGCAGATGCAAATGCAGCAGCTGAGGCGAGAAAGAGGAGGAAAAATCTCACGAAGCTAAAGAGCCTTCATGGCCGGCAGTGTCGGACACAGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
bZIP_1

Protein Analysis

546

Amino Acids

59.52

Weight (kDa)

8.33

Isoelectric Point (pI)

60.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bZIP_1 PF00170 185 - 247 7.7e-06 bZIP transcription factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 788
AatII GACGTC 1 cut(s) 625
Acc16I TGCGCA 1 cut(s) 1206
Acc36I ACCTGC 1 cut(s) 788
AccB1I GGYRCC 1 cut(s) 221
AccB7I CCANNNNNTGG 1 cut(s) 727
AccBSI CCGCTC 1 cut(s) 565
AccI GTMKAC 2 cut(s) 95, 619
AccII CGCG 1 cut(s) 210
AccIII TCCGGA 2 cut(s) 337, 1406
AclWI GGATC 4 cut(s) 150, 348, 360, 458
AcoI YGGCCR 1 cut(s) 1612
AcsI RAATTY 2 cut(s) 538, 853
AcyI GRCGYC 2 cut(s) 222, 622
AfaI GTAC 1 cut(s) 311
AfiI CCNNNNNNNGG 6 cut(s) 18, 77, 727, 995, 1322, 1387
AflIII ACRYGT 1 cut(s) 332
AgsI TTSAA 8 cut(s) 751, 924, 1019, 1138, 1194, 1266, 1271, 1445
AjnI CCWGG 1 cut(s) 915
Alw21I GWGCWC 3 cut(s) 35, 165, 1054
Alw26I GTCTC 2 cut(s) 291, 1292
AlwI GGATC 4 cut(s) 150, 348, 360, 458
AlwNI CAGNNNCTG 1 cut(s) 1562
Ama87I CYCGRG 1 cut(s) 164
Aor13HI TCCGGA 2 cut(s) 337, 1406
AoxI GGCC 2 cut(s) 902, 1612
ApeKI GCWGC 5 cut(s) 522, 656, 1001, 1556, 1559
ApoI RAATTY 2 cut(s) 538, 853
ArsI GACNNNNNNTTYG 6 cut(s) 581, 597, 601, 613, 629, 633
AspLEI GCGC 5 cut(s) 181, 196, 210, 224, 1207
AspS9I GGNCC 3 cut(s) 42, 937, 1343
AsuC2I CCSGG 2 cut(s) 265, 323
AsuHPI GGTGA 8 cut(s) 163, 373, 427, 497, 781, 812, 1340, 1340
AsuNHI GCTAGC 1 cut(s) 703
AvaI CYCGRG 1 cut(s) 164
AvaII GGWCC 3 cut(s) 42, 937, 1343
AxyI CCTNAGG 1 cut(s) 348
BaeI ACNNNNGTAYC 2 cut(s) 824, 857
BanI GGYRCC 1 cut(s) 221
BanII GRGCYC 3 cut(s) 35, 165, 1054
BbsI GAAGAC 3 cut(s) 425, 834, 1116
Bbv12I GWGCWC 3 cut(s) 35, 165, 1054
BbvCI CCTCAGC 1 cut(s) 1563
BbvI GCAGC 5 cut(s) 534, 643, 1013, 1568, 1571
BccI CCATC 3 cut(s) 142, 1316, 1348
BceAI ACGGC 1 cut(s) 63
BcgI CGANNNNNNTGC 4 cut(s) 382, 416, 1286, 1320
BciT130I CCWGG 1 cut(s) 917
BclI TGATCA 1 cut(s) 1330
BcnI CCSGG 2 cut(s) 265, 323
BcoDI GTCTC 2 cut(s) 291, 1292
BfaI CTAG 3 cut(s) 603, 704, 1533
BfoI RGCGCY 2 cut(s) 182, 225
BfuAI ACCTGC 1 cut(s) 788
BglI GCCNNNNNGGC 2 cut(s) 665, 1611
BlpI GCTNAGC 1 cut(s) 456
Bme1390I CCNGG 3 cut(s) 265, 323, 917
Bme18I GGWCC 3 cut(s) 42, 937, 1343
BmeT110I CYCGRG 1 cut(s) 164
BmgT120I GGNCC 3 cut(s) 42, 937, 1343
BmiI GGNNCC 3 cut(s) 43, 223, 327
BmrFI CCNGG 3 cut(s) 265, 323, 917
BmrI ACTGGG 1 cut(s) 1114
BmsI GCATC 5 cut(s) 195, 816, 1400, 1424, 1537
BmtI GCTAGC 1 cut(s) 707
BmuI ACTGGG 1 cut(s) 1114
BpiI GAAGAC 3 cut(s) 425, 834, 1116
BplI GAGNNNNNCTC 2 cut(s) 271, 303
Bpu10I CCTNAGC 1 cut(s) 1563
Bpu1102I GCTNAGC 1 cut(s) 456
BpuMI CCSGG 2 cut(s) 265, 323
BsaBI GATNNNNATC 1 cut(s) 1444
BsaHI GRCGYC 2 cut(s) 222, 622
BsaI GGTCTC 2 cut(s) 291, 1292
BsaJI CCNNGG 3 cut(s) 12, 45, 1076
BsaWI WCCGGW 3 cut(s) 77, 337, 1406
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bsc4I CCNNNNNNNGG 6 cut(s) 18, 77, 727, 995, 1322, 1387
Bse118I RCCGGY 2 cut(s) 988, 1614
Bse1I ACTGG 5 cut(s) 417, 721, 1120, 1171, 1222
Bse21I CCTNAGG 1 cut(s) 348
Bse3DI GCAATG 2 cut(s) 383, 983
Bse8I GATNNNNATC 1 cut(s) 1444
BseAI TCCGGA 2 cut(s) 337, 1406
BseBI CCWGG 1 cut(s) 917
BseDI CCNNGG 3 cut(s) 12, 45, 1076
BseGI GGATG 5 cut(s) 153, 790, 912, 1083, 1327
BseJI GATNNNNATC 1 cut(s) 1444
BseLI CCNNNNNNNGG 6 cut(s) 18, 77, 727, 995, 1322, 1387
BseMI GCAATG 2 cut(s) 383, 983
BseMII CTCAG 8 cut(s) 362, 414, 425, 447, 537, 947, 1322, 1554
BseNI ACTGG 5 cut(s) 417, 721, 1120, 1171, 1222
BseRI GAGGAG 4 cut(s) 173, 563, 653, 1591
BseXI GCAGC 5 cut(s) 534, 643, 1013, 1568, 1571
BseYI CCCAGC 1 cut(s) 233
BsgI GTGCAG 2 cut(s) 842, 1020
Bsh1236I CGCG 1 cut(s) 210
Bsh1285I CGRYCG 2 cut(s) 23, 111
BshFI GGCC 2 cut(s) 904, 1614
BshNI GGYRCC 1 cut(s) 221
BsiEI CGRYCG 2 cut(s) 23, 111
BsiHKAI GWGCWC 3 cut(s) 35, 165, 1054
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 7 cut(s) 78, 264, 323, 338, 989, 1407, 1615
BslFI GGGAC 1 cut(s) 864
BslI CCNNNNNNNGG 6 cut(s) 18, 77, 727, 995, 1322, 1387
BsmAI GTCTC 2 cut(s) 291, 1292
BsmFI GGGAC 1 cut(s) 864
BsnI GGCC 2 cut(s) 904, 1614
Bso31I GGTCTC 2 cut(s) 291, 1292
BsoBI CYCGRG 1 cut(s) 164
Bsp1286I GDGCHC 3 cut(s) 35, 165, 1054
Bsp13I TCCGGA 2 cut(s) 337, 1406
Bsp143I GATC 7 cut(s) 142, 340, 352, 463, 661, 1288, 1330
Bsp1720I GCTNAGC 1 cut(s) 456
BspANI GGCC 2 cut(s) 904, 1614
BspCNI CTCAG 8 cut(s) 361, 415, 426, 448, 538, 946, 1321, 1555
BspEI TCCGGA 2 cut(s) 337, 1406
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 3 cut(s) 43, 223, 327
BspMI ACCTGC 1 cut(s) 788
BspOI GCTAGC 1 cut(s) 707
BspPI GGATC 4 cut(s) 150, 348, 360, 458
BspQI GCTCTTC 1 cut(s) 555
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 2 cut(s) 291, 1292
BsrBI CCGCTC 1 cut(s) 565
BsrDI GCAATG 2 cut(s) 383, 983
BsrFI RCCGGY 2 cut(s) 988, 1614
BsrI ACTGG 5 cut(s) 417, 721, 1120, 1171, 1222
BssAI RCCGGY 2 cut(s) 988, 1614
BssECI CCNNGG 3 cut(s) 12, 45, 1076
BssMI GATC 7 cut(s) 142, 340, 352, 463, 661, 1288, 1330
BssNI GRCGYC 2 cut(s) 222, 622
BssT1I CCWWGG 1 cut(s) 1076
Bst2UI CCWGG 1 cut(s) 917
Bst4CI ACNGT 7 cut(s) 104, 388, 878, 1201, 1281, 1459, 1632
Bst6I CTCTTC 2 cut(s) 555, 1015
BstACI GRCGYC 2 cut(s) 222, 622
BstC8I GCNNGC 5 cut(s) 183, 261, 705, 990, 1616
BstDEI CTNAG 9 cut(s) 348, 423, 434, 456, 546, 765, 933, 1308, 1563
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 5 cut(s) 153, 790, 912, 1083, 1327
BstFNI CGCG 1 cut(s) 210
BstH2I RGCGCY 2 cut(s) 182, 225
BstHHI GCGC 5 cut(s) 181, 196, 210, 224, 1207
BstKTI GATC 7 cut(s) 145, 343, 355, 466, 664, 1291, 1333
BstMAI GTCTC 2 cut(s) 291, 1292
BstMBI GATC 7 cut(s) 142, 340, 352, 463, 661, 1288, 1330
BstMCI CGRYCG 2 cut(s) 23, 111
BstNI CCWGG 1 cut(s) 917
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 3 cut(s) 263, 321, 915
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 5 cut(s) 534, 643, 1013, 1568, 1571
BstV2I GAAGAC 3 cut(s) 425, 834, 1116
BstX2I RGATCY 1 cut(s) 340
BstXI CCANNNNNNTGG 1 cut(s) 900
BstYI RGATCY 1 cut(s) 340
Bsu36I CCTNAGG 1 cut(s) 348
BsuRI GGCC 2 cut(s) 904, 1614
BtgI CCRYGG 1 cut(s) 45
BtgZI GCGATG 1 cut(s) 218
BtsCI GGATG 5 cut(s) 153, 790, 912, 1083, 1327
BtsI GCAGTG 1 cut(s) 1625
BtsIMutI CAGTG 4 cut(s) 1183, 1455, 1625, 1637
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 5 cut(s) 183, 261, 705, 990, 1616
CaiI CAGNNNCTG 1 cut(s) 1562
CfoI GCGC 5 cut(s) 181, 196, 210, 224, 1207
Cfr10I RCCGGY 2 cut(s) 988, 1614
Cfr13I GGNCC 3 cut(s) 42, 937, 1343
Csp6I GTAC 1 cut(s) 310
CviAII CATG 4 cut(s) 333, 461, 1284, 1610
CviQI GTAC 1 cut(s) 310
DdeI CTNAG 9 cut(s) 348, 423, 434, 456, 546, 765, 933, 1308, 1563
DinI GGCGCC 1 cut(s) 223
DpnI GATC 7 cut(s) 144, 342, 354, 465, 663, 1290, 1332
DpnII GATC 7 cut(s) 142, 340, 352, 463, 661, 1288, 1330
EaeI YGGCCR 1 cut(s) 1612
Eam1104I CTCTTC 2 cut(s) 555, 1015
EarI CTCTTC 2 cut(s) 555, 1015
EciI GGCGGA 3 cut(s) 188, 279, 550
Ecl136II GAGCTC 3 cut(s) 33, 163, 1052
Eco130I CCWWGG 1 cut(s) 1076
Eco24I GRGCYC 3 cut(s) 35, 165, 1054
Eco31I GGTCTC 2 cut(s) 291, 1292
Eco47I GGWCC 3 cut(s) 42, 937, 1343
Eco53kI GAGCTC 3 cut(s) 33, 163, 1052
Eco81I CCTNAGG 1 cut(s) 348
Eco88I CYCGRG 1 cut(s) 164
EcoICRI GAGCTC 3 cut(s) 33, 163, 1052
EcoO109I RGGNCCY 1 cut(s) 1343
EcoRII CCWGG 1 cut(s) 915
EcoT14I CCWWGG 1 cut(s) 1076
EcoT38I GRGCYC 3 cut(s) 35, 165, 1054
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 1076
FaeI CATG 4 cut(s) 336, 464, 1287, 1613
FalI AAGNNNNNCTT 2 cut(s) 493, 525
FaqI GGGAC 1 cut(s) 864
FatI CATG 4 cut(s) 332, 460, 1283, 1609
FauI CCCGC 2 cut(s) 46, 321
FbaI TGATCA 1 cut(s) 1330
FblI GTMKAC 2 cut(s) 95, 619
FokI GGATG 5 cut(s) 160, 797, 899, 1070, 1334
FriOI GRGCYC 3 cut(s) 35, 165, 1054
FspBI CTAG 3 cut(s) 603, 704, 1533
FspI TGCGCA 1 cut(s) 1206
GlaI GCGC 5 cut(s) 180, 195, 209, 223, 1206
GsaI CCCAGC 1 cut(s) 237
HaeII RGCGCY 2 cut(s) 182, 225
HaeIII GGCC 2 cut(s) 904, 1614
HapII CCGG 7 cut(s) 78, 264, 323, 338, 989, 1407, 1615
HhaI GCGC 5 cut(s) 181, 196, 210, 224, 1207
Hin1I GRCGYC 2 cut(s) 222, 622
Hin1II CATG 4 cut(s) 336, 464, 1287, 1613
Hin6I GCGC 5 cut(s) 179, 194, 208, 222, 1205
HinP1I GCGC 5 cut(s) 179, 194, 208, 222, 1205
HincII GTYRAC 3 cut(s) 96, 620, 645
HindII GTYRAC 3 cut(s) 96, 620, 645
HinfI GANTC 9 cut(s) 74, 227, 273, 295, 596, 1007, 1110, 1256, 1445
HpaI GTTAAC 1 cut(s) 645
HpaII CCGG 7 cut(s) 78, 264, 323, 338, 989, 1407, 1615
HphI GGTGA 8 cut(s) 163, 373, 427, 497, 781, 812, 1340, 1340
Hpy166II GTNNAC 5 cut(s) 45, 96, 620, 645, 1514
Hpy188I TCNGA 8 cut(s) 13, 147, 435, 687, 759, 1450, 1470, 1626
Hpy8I GTNNAC 5 cut(s) 45, 96, 620, 645, 1514
Hpy99I CGWCG 8 cut(s) 40, 67, 70, 94, 97, 100, 621, 624
HpyAV CCTTC 6 cut(s) 519, 915, 1265, 1272, 1392, 1616
HpyCH4III ACNGT 7 cut(s) 104, 388, 878, 1201, 1281, 1459, 1632
HpyCH4IV ACGT 1 cut(s) 622
HpyF3I CTNAG 9 cut(s) 348, 423, 434, 456, 546, 765, 933, 1308, 1563
HpySE526I ACGT 1 cut(s) 622
Hsp92I GRCGYC 2 cut(s) 222, 622
Hsp92II CATG 4 cut(s) 336, 464, 1287, 1613
HspAI GCGC 5 cut(s) 179, 194, 208, 222, 1205
KasI GGCGCC 1 cut(s) 221
Kpn2I TCCGGA 2 cut(s) 337, 1406
KroI GCCGGC 2 cut(s) 988, 1614
KroNI GCCGGC 2 cut(s) 990, 1616
Ksp22I TGATCA 1 cut(s) 1330
KspAI GTTAAC 1 cut(s) 645
Kzo9I GATC 7 cut(s) 142, 340, 352, 463, 661, 1288, 1330
LguI GCTCTTC 1 cut(s) 555
LmnI GCTCC 5 cut(s) 160, 331, 700, 1043, 1057
Lsp1109I GCAGC 5 cut(s) 534, 643, 1013, 1568, 1571
LweI GCATC 5 cut(s) 195, 816, 1400, 1424, 1537
MaeI CTAG 3 cut(s) 603, 704, 1533
MaeII ACGT 1 cut(s) 622
MaeIII GTNAC 4 cut(s) 361, 403, 503, 1459
MalI GATC 7 cut(s) 144, 342, 354, 465, 663, 1290, 1332
MbiI CCGCTC 1 cut(s) 565
MboI GATC 7 cut(s) 142, 340, 352, 463, 661, 1288, 1330
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 3 cut(s) 35, 165, 1054
MluCI AATT 7 cut(s) 238, 470, 538, 612, 853, 1183, 1266
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 6 cut(s) 68, 282, 304, 605, 1001, 1104
MmeI TCCRAC 3 cut(s) 1138, 1191, 1604
MroI TCCGGA 2 cut(s) 337, 1406
MroNI GCCGGC 2 cut(s) 988, 1614
MseI TTAA 7 cut(s) 473, 542, 644, 873, 1164, 1244, 1293
MspA1I CMGCKG 3 cut(s) 233, 1004, 1562
MspI CCGG 7 cut(s) 78, 264, 323, 338, 989, 1407, 1615
MspR9I CCNGG 3 cut(s) 265, 323, 917
MunI CAATTG 1 cut(s) 1266
MvaI CCWGG 1 cut(s) 917
MvnI CGCG 1 cut(s) 210
NaeI GCCGGC 2 cut(s) 990, 1616
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 2 cut(s) 265, 323
NdeII GATC 7 cut(s) 142, 340, 352, 463, 661, 1288, 1330
NgoMIV GCCGGC 2 cut(s) 988, 1614
NheI GCTAGC 1 cut(s) 703
NlaIII CATG 4 cut(s) 336, 464, 1287, 1613
NlaIV GGNNCC 3 cut(s) 43, 223, 327
NmeAIII GCCGAG 1 cut(s) 318
NmuCI GTSAC 2 cut(s) 361, 503
NsbI TGCGCA 1 cut(s) 1206
NspI RCATGY 1 cut(s) 336
PaeR7I CTCGAG 1 cut(s) 164
PaqCI CACCTGC 1 cut(s) 788
PciI ACATGT 1 cut(s) 332
PciSI GCTCTTC 1 cut(s) 555
PcsI WCGNNNNNNNCGW 2 cut(s) 95, 108
PdiI GCCGGC 2 cut(s) 990, 1616
PfeI GAWTC 3 cut(s) 227, 1256, 1445
PflFI GACNNNGTC 1 cut(s) 40
PflMI CCANNNNNTGG 1 cut(s) 727
PleI GAGTC 6 cut(s) 68, 281, 303, 604, 1001, 1104
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 6 cut(s) 68, 281, 303, 604, 1001, 1104
PpuMI RGGWCCY 1 cut(s) 1343
PscI ACATGT 1 cut(s) 332
Psp124BI GAGCTC 3 cut(s) 35, 165, 1054
Psp5II RGGWCCY 1 cut(s) 1343
Psp6I CCWGG 1 cut(s) 915
PspFI CCCAGC 1 cut(s) 233
PspGI CCWGG 1 cut(s) 915
PspN4I GGNNCC 3 cut(s) 43, 223, 327
PspPI GGNCC 3 cut(s) 42, 937, 1343
PspPPI RGGWCCY 1 cut(s) 1343
PspXI VCTCGAGB 1 cut(s) 164
PstNI CAGNNNCTG 1 cut(s) 1562
PsuI RGATCY 1 cut(s) 340
PsyI GACNNNGTC 1 cut(s) 40
PvuII CAGCTG 2 cut(s) 1004, 1562
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
SacI GAGCTC 3 cut(s) 35, 165, 1054
SalI GTCGAC 2 cut(s) 94, 618
SapI GCTCTTC 1 cut(s) 555
SaqAI TTAA 7 cut(s) 473, 542, 644, 873, 1164, 1244, 1293
Sau3AI GATC 7 cut(s) 142, 340, 352, 463, 661, 1288, 1330
Sau96I GGNCC 3 cut(s) 42, 937, 1343
SchI GAGTC 6 cut(s) 68, 282, 304, 605, 1001, 1104
ScrFI CCNGG 3 cut(s) 265, 323, 917
SduI GDGCHC 3 cut(s) 35, 165, 1054
SfaNI GCATC 5 cut(s) 195, 816, 1400, 1424, 1537
SfoI GGCGCC 1 cut(s) 223
Sfr274I CTCGAG 1 cut(s) 164
SgrDI CGTCGACG 2 cut(s) 94, 618
SinI GGWCC 3 cut(s) 42, 937, 1343
SlaI CTCGAG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 7 cut(s) 238, 470, 538, 612, 853, 1183, 1266
SspDI GGCGCC 1 cut(s) 221
SspI AATATT 1 cut(s) 1377
SspMI CTAG 3 cut(s) 603, 704, 1533
SstI GAGCTC 3 cut(s) 35, 165, 1054
StyD4I CCNGG 3 cut(s) 263, 321, 915
StyI CCWWGG 1 cut(s) 1076
TaaI ACNGT 7 cut(s) 104, 388, 878, 1201, 1281, 1459, 1632
TaiI ACGT 1 cut(s) 625
TaqI TCGA 7 cut(s) 29, 35, 95, 111, 165, 592, 619
TasI AATT 7 cut(s) 238, 470, 538, 612, 853, 1183, 1266
TauI GCSGC 7 cut(s) 53, 132, 135, 173, 222, 376, 1391
TfiI GAWTC 3 cut(s) 227, 1256, 1445
Tru1I TTAA 7 cut(s) 473, 542, 644, 873, 1164, 1244, 1293
Tru9I TTAA 7 cut(s) 473, 542, 644, 873, 1164, 1244, 1293
TscAI CASTG 4 cut(s) 1183, 1462, 1625, 1637
TseFI GTSAC 2 cut(s) 361, 503
TseI GCWGC 5 cut(s) 522, 656, 1001, 1556, 1559
Tsp45I GTSAC 2 cut(s) 361, 503
TspDTI ATGAA 6 cut(s) 501, 693, 834, 1072, 1176, 1598
TspGWI ACGGA 4 cut(s) 828, 960, 1059, 1452
TspRI CASTG 4 cut(s) 1183, 1462, 1625, 1637
Tth111I GACNNNGTC 1 cut(s) 40
Van91I CCANNNNNTGG 1 cut(s) 727
VpaK11BI GGWCC 3 cut(s) 42, 937, 1343
XapI RAATTY 2 cut(s) 538, 853
XceI RCATGY 1 cut(s) 336
XhoI CTCGAG 1 cut(s) 164
XmiI GTMKAC 2 cut(s) 95, 619
XspI CTAG 3 cut(s) 603, 704, 1533
ZraI GACGTC 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.