MD12G1030900.v1.1

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016146
Physical Location & Seq
Reverse (-)
3429752 .. 3432621
2870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1030900.v1.1.491

Sequence Viewer

Length: 495 bp
ATGTGGGCAAGCAAAGAAGGAGGGCCGCCGGAGGTCACTCTAGAAACCACCATTGGCAACTTCACCGTCGAGCTTTACTACAAGCACGCGCCGAGGACTTGCAGGAACTTCATCGAGCTCGCTCGCAGGGGGTATTACGACAATGTCAAATTCCACAGAATCATCAAGGATTTCATAGTGCAAGGTGGTGATCCCACTGGGACAGGAAGGGGTGGAGAATCCATTTATGGGGGAAAGTTTGAGGATGAGATAAAACAAGAGTTGAAGCATACTGGAGCTGGCATCTTATCGATGGCGAATGCTGGTCCAAATACAAATGGAAGCCAGTTCTTTATCACATTGGCACCGTGCCCGTCACTGGACGGAAAGCACACAATATTTGGAAGGATCTGCACAGGAATGGAGTTCATCAAAAGACTTGGCAGTGTCCAAACTGATAATACTGATAGGCCGGTGCATGATGTGAAGATACTACGAACATCTGTCAAAGATTAG

Protein Analysis

165

Amino Acids

18.17

Weight (kDa)

8.43

Isoelectric Point (pI)

26.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 12 - 162 3.6e-55 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36130
fragaria_vesca FvH4_6g19480
malus_domestica MD12G1030900.v1.1 MD14G1031400.v1.1
prunus_persica Prupe.7G101500_v2.0.a1
pyrus_communis pycom12g02890 pycom14g02830
rosa_chinensis RchiOBHm_Chr3g0474811
rosa_laevigata RLG00000023893
rosa_multiflora Rmu_sc0004848.1_g000010
rosa_roxburghii Rroxscaffold_6G00406830
rosa_rugosa Rorug03G0144100
rosa_samantha Rh3BG223700 Rh3CG218600
rosa_wichuraiana Rw3G017650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 343
AccII CGCG 1 cut(s) 89
AciI CCGC 1 cut(s) 26
AclWI GGATC 2 cut(s) 185, 395
AcsI RAATTY 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 228, 358
AgsI TTSAA 1 cut(s) 265
AjuI GAANNNNNNNTTGG 2 cut(s) 36, 68
AluBI AGCT 3 cut(s) 73, 118, 278
AluI AGCT 3 cut(s) 73, 118, 278
Alw21I GWGCWC 1 cut(s) 120
AlwI GGATC 2 cut(s) 185, 395
AoxI GGCC 2 cut(s) 23, 449
ApoI RAATTY 1 cut(s) 149
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 2 cut(s) 23, 305
AsuHPI GGTGA 2 cut(s) 55, 200
AvaII GGWCC 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 353
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 120
BccI CCATC 1 cut(s) 286
BfaI CTAG 1 cut(s) 41
BisI GCNGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 27
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 23, 305
BmiI GGNNCC 1 cut(s) 345
BmrI ACTGGG 1 cut(s) 207
BmsI GCATC 1 cut(s) 291
BmuI ACTGGG 1 cut(s) 207
BpmI CTGGAG 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 290
BsaJI CCNNGG 1 cut(s) 92
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 358
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 4 cut(s) 202, 277, 325, 363
BseCI ATCGAT 1 cut(s) 290
BseDI CCNNGG 1 cut(s) 92
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 2 cut(s) 228, 358
BseNI ACTGG 4 cut(s) 202, 277, 325, 363
BseSI GKGCMC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 376
Bsh1236I CGCG 1 cut(s) 89
BshFI GGCC 2 cut(s) 25, 451
BshNI GGYRCC 1 cut(s) 343
BshVI ATCGAT 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 2 cut(s) 29, 452
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 2 cut(s) 228, 358
BsmFI GGGAC 1 cut(s) 214
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 2 cut(s) 25, 451
Bsp1286I GDGCHC 2 cut(s) 120, 353
Bsp143I GATC 2 cut(s) 190, 387
BspACI CCGC 1 cut(s) 26
BspANI GGCC 2 cut(s) 25, 451
BspDI ATCGAT 1 cut(s) 290
BspFNI CGCG 1 cut(s) 89
BspLI GGNNCC 1 cut(s) 345
BspPI GGATC 2 cut(s) 185, 395
BspT107I GGYRCC 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 4 cut(s) 202, 277, 325, 363
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 1 cut(s) 92
BssMI GATC 2 cut(s) 190, 387
Bst4CI ACNGT 2 cut(s) 67, 348
BstC8I GCNNGC 5 cut(s) 10, 87, 120, 124, 280
BstF5I GGATG 1 cut(s) 250
BstFNI CGCG 1 cut(s) 89
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 2 cut(s) 193, 390
BstMBI GATC 2 cut(s) 190, 387
BstSLI GKGCMC 1 cut(s) 353
BstUI CGCG 1 cut(s) 89
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
Bsu15I ATCGAT 1 cut(s) 290
BsuRI GGCC 2 cut(s) 25, 451
BsuTUI ATCGAT 1 cut(s) 290
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 430
BtsIMutI CAGTG 3 cut(s) 195, 356, 430
Cac8I GCNNGC 5 cut(s) 10, 87, 120, 124, 280
CfoI GCGC 1 cut(s) 91
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 2 cut(s) 23, 305
ClaI ATCGAT 1 cut(s) 290
CviAII CATG 1 cut(s) 458
CviJI RGCY 6 cut(s) 25, 73, 118, 278, 324, 451
CviKI_1 RGCY 6 cut(s) 25, 73, 118, 278, 324, 451
DpnI GATC 2 cut(s) 192, 389
DpnII GATC 2 cut(s) 190, 387
Ecl136II GAGCTC 1 cut(s) 118
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 118
EcoT38I GRGCYC 1 cut(s) 120
FaeI CATG 1 cut(s) 461
FaiI YATR 4 cut(s) 176, 228, 270, 459
FaqI GGGAC 1 cut(s) 214
FatI CATG 1 cut(s) 457
Fnu4HI GCNGC 1 cut(s) 26
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 1 cut(s) 26
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 90
GluI GCNGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 2 cut(s) 25, 451
HapII CCGG 2 cut(s) 29, 452
HhaI GCGC 1 cut(s) 91
Hin1II CATG 1 cut(s) 461
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HinfI GANTC 2 cut(s) 159, 218
HpaII CCGG 2 cut(s) 29, 452
HphI GGTGA 2 cut(s) 55, 200
Hpy188III TCNNGA 1 cut(s) 41
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 3 cut(s) 11, 201, 378
HpyCH4III ACNGT 2 cut(s) 67, 348
HpyCH4V TGCA 4 cut(s) 102, 181, 393, 457
Hsp92II CATG 1 cut(s) 461
HspAI GCGC 1 cut(s) 89
Kzo9I GATC 2 cut(s) 190, 387
LmnI GCTCC 1 cut(s) 275
LweI GCATC 1 cut(s) 291
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 2 cut(s) 34, 354
MalI GATC 2 cut(s) 192, 389
MboI GATC 2 cut(s) 190, 387
MboII GAAGA 1 cut(s) 478
MflI RGATCY 1 cut(s) 387
MhlI GDGCHC 2 cut(s) 120, 353
MluCI AATT 1 cut(s) 149
MnlI CCTC 4 cut(s) 14, 25, 87, 235
MslI CAYNNNNRTG 1 cut(s) 398
MspI CCGG 2 cut(s) 29, 452
Mva1269I GAATGC 1 cut(s) 304
MvnI CGCG 1 cut(s) 89
NdeII GATC 2 cut(s) 190, 387
NlaIII CATG 1 cut(s) 461
NlaIV GGNNCC 1 cut(s) 345
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 2 cut(s) 34, 354
PctI GAATGC 1 cut(s) 304
PfeI GAWTC 2 cut(s) 159, 218
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 1 cut(s) 27
Psp124BI GAGCTC 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 345
PspPI GGNCC 2 cut(s) 23, 305
PsuI RGATCY 1 cut(s) 387
PsyI GACNNNGTC 1 cut(s) 143
RseI CAYNNNNRTG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 120
SatI GCNGC 1 cut(s) 26
Sau3AI GATC 2 cut(s) 190, 387
Sau96I GGNCC 2 cut(s) 23, 305
SduI GDGCHC 2 cut(s) 120, 353
SetI ASST 5 cut(s) 36, 75, 120, 187, 280
SfaNI GCATC 1 cut(s) 291
SinI GGWCC 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 398
Sse9I AATT 1 cut(s) 149
SsiI CCGC 1 cut(s) 26
SspI AATATT 1 cut(s) 378
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 120
TaaI ACNGT 2 cut(s) 67, 348
TaqI TCGA 3 cut(s) 69, 114, 290
TasI AATT 1 cut(s) 149
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 2 cut(s) 159, 218
TscAI CASTG 3 cut(s) 202, 363, 430
TseFI GTSAC 2 cut(s) 34, 354
Tsp45I GTSAC 2 cut(s) 34, 354
TspDTI ATGAA 3 cut(s) 100, 163, 397
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 3 cut(s) 202, 363, 430
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 305
XapI RAATTY 1 cut(s) 149
XbaI TCTAGA 1 cut(s) 40
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.