Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0016146

Physical Location & Seq

Genomic Coordinates Forward (+)

11817129 .. 11822586

Gene Structure 5458 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0144100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2340 bp

ATGGAACCTATGGTTCTGAACCTAACCAGCATGAGAACTTCTTCAGATGGAGCTTGGCAGGGTGATAATCCTCTGAACCATGCCTTCCCATTGTTGATAGTTCAAACCACTCTGGTCCTCTTCATCAGTCGCTTCTTCGCCTTCATTCTCAAACCACTCCGCCAACCCAAGGTCATTGCCGAGATCTTAGGTGGGATTTTGCTAGGACCTTCTGCACTTGGCCGGATCAAAGATTTCTCCCCCATCATATTCCCTTCATGGAGCACTCCCATACTCGAATCAGTAGCAAGCATCGGCCTCCTCTTCTACCTATTCCTCGTAGGCCTCGAGCTCGACTTGCGCTCCATTCGCCGCAGTGGCAAAAGCGCCATCACCATAGCACTCGCAGGCATTTCCCTGCCTTTCCTCATCGCTGTAGGAGTCACATTCCTCATGCGCAAGGCCATCAACGGTGAGAACAAAGTAGGTTACCCTCAGCTCCTCATGTTCATAGGGGTAGCCCTCTCCATCACCGCCTTCCCTGTCCTCGCACGCATCTTAGCAGAGCTCAAACTGCTCACCACACGCATAGGTGAGACGGCAATGGCTGCAGCAGCCTTCAACGACGTGGCTGCATGGATTCTGCTGGCCTTGGCCGTGGCCTTAGCCGGTGGGAACCATAAAGGCCCTCTAGTCTCCATTTGGGTTCTCATTTCCGGCGTGGCTTTTGTTGCTTTTATGTTCATTCTGGTCCGTCCTTTGATGACTTGGGTTGCTAACCGCTGCTCATCTCAAATGGAGGACGAAGCTTATATATGCTTAACCCTAGCCGGGGTCATGTTGTCCGGGTTCATGACTGACCTAATCGGTGTCCATGCCATCTTTGGGGCATTTATTTTTGGATTAACCATTCCGAAAGGGGGTGAATTCGCAGCCAAACTGACTAAAAGGATCGAGGATTTTGTGTCTGGTTTGCTTCTCCCTCTCTACTTTGCCTCGAGTGGTTTGAAGACTGACGTTACTAAAATATGTGGGATCAAGGCATGGGGTCTTTTGGCTTTGGTTATATCAGTTTCTTGCACTGGAAAAATTTTGGGAACGTTTTTGGTTGCAATGATGTGTATGATACCAGTGAGGGAGTCATTAGCTCTAGGTGTCCTGATGAATACTAAAGGATTGGTGGAGCTTATTGTCCTGAATATTGGCAAGGAGAAAAAGATTCTAAACGACGAGATGTTTGCTATTTTTGTGCTGATGGCACTTTTCACCACCTTCATGACGACACCAATAGTGATGGCCATTTACAAACCCTATGTCGTTGCTTCCCAATCTGAGAGTAGTTTAGAGAGACAGAATTCTCCCAGCAAGAACTCGAATAAAAAGAAGGAACTTCGAATCCTTGCTTGTTTTCATGGACGGGGAAGTGTACCCTCCATTATCAACCTCATTGAGTCGACCGGAACCTCCGAGAATTCTCTTCTCAAAGTTTACGTGATGCATCTTGTTGAACTTACTGATCGTTCATCCTCCATTATGATGGTACACAAAGTACGGAAAAACGGCTTGCCCTTCATCAACCGCTTTTGTCTAGGGGTGGCACGCCATATCGCCTCAGCATTTGAAGCTTATAGTCAAGTTGGTCAAGTCACCATCCGTCACACAACAGCCATCTCGGCTTTGTCTACGATGCACGAGGATATTCTCCATGTTGCGGAAGCCAAGAGGGTGTCCATCATTATCTTGCCTTTCAACAAACAGGGGAATAAACAGGGTGAAGAGACGATGGATAATTTAGAGCATCGCTGGAGAGCAGTCAATGAGAGAGTTCTAAAATGTGCTAATTGCTCAGTAGCATTGTTTGTGGATCGTGGACTCGGTGTTGGAGCTGAGAAGGGTGCAAAGACTACCAATCTGAAGAGGGTGTGCATTCTATTCTTCGGTGGAACCGATGACCGCAGGGCACTGCAGTATGTTGCGGGAATTGCAAATCAGAGCGTTGAAGTTACCGTTGTGAGGTGTTTGGAGCACAGCACTAATCCTCTAAGCCCCATCACCAACCCCGACAGGGATCTTGATGAGACTGCAGTGGCAGAGTTTCGAAGGCAGAATGGGTCGGTGGAATTCATAGAGAAGGTGGTAGTCGGCAACATTATAGACACGGCGGCGGGCATAGTTAAGGGCAGGGAATACAACCTGGTGATCGTGGGGAACCAGCACCACCCTTCAACAATTGTTGCAGAATTGGCTCACAATCAGCTGGAGCATACCGAACTGGGACCAATTGGAGACGCATTAGCTTCATCCTCACATGGGATTTCGTGCTCCGTGCTTGTGATACAAACACCACAGATTAGCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000413 GO:0003674 GO:0003755 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016859 GO:0018193 GO:0018208 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

Pro_isomerase

Protein Analysis

779

Amino Acids

84.64

Weight (kDa)

9.04

Isoelectric Point (pI)

35.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	41 - 424	1.4e-62	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	487 - 627	4.1e-34	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	634 - 773	2.3e-09	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016146)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36130
fragaria_vesca	FvH4_6g19480
malus_domestica	MD12G1030900.v1.1 MD14G1031400.v1.1
prunus_persica	Prupe.7G101500_v2.0.a1
pyrus_communis	pycom12g02890 pycom14g02830
rosa_chinensis	RchiOBHm_Chr3g0474811
rosa_laevigata	RLG00000023893
rosa_multiflora	Rmu_sc0004848.1_g000010
rosa_roxburghii	Rroxscaffold_6G00406830
rosa_rugosa	Rorug03G0144100
rosa_samantha	Rh3BG223700 Rh3CG218600
rosa_wichuraiana	Rw3G017650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	437
AccI	GTMKAC	2 cut(s)	1433, 1661
AclI	AACGTT	1 cut(s)	1079
AclWI	GGATC	5 cut(s)	233, 938, 1022, 1851, 2055
AcoI	YGGCCR	3 cut(s)	220, 633, 1275
AcsI	RAATTY	5 cut(s)	905, 1068, 1333, 1450, 2099
AcuI	CTGAAG	2 cut(s)	27, 1913
AfaI	GTAC	3 cut(s)	1407, 1521, 1530
AfiI	CCNNNNNNNGG	9 cut(s)	169, 810, 811, 864, 899, 1690, 1992, 2044, 2289
AgsI	TTSAA	8 cut(s)	104, 601, 988, 1487, 1601, 1729, 1979, 2205
AjiI	CACGTC	1 cut(s)	607
AjnI	CCWGG	1 cut(s)	2172
AloI	GAACNNNNNNTCC	2 cut(s)	1359, 1391
Alw21I	GWGCWC	5 cut(s)	266, 333, 549, 2007, 2303
Alw26I	GTCTC	6 cut(s)	569, 679, 1321, 1751, 2051, 2259
AlwI	GGATC	5 cut(s)	233, 938, 1022, 1851, 2055
Ama87I	CYCGRG	2 cut(s)	326, 976
AoxI	GGCC	9 cut(s)	220, 295, 322, 441, 627, 633, 639, 664, 1275
ApeKI	GCWGC	6 cut(s)	587, 590, 593, 611, 762, 911
ApoI	RAATTY	5 cut(s)	905, 1068, 1333, 1450, 2099
ArsI	GACNNNNNNTTYG	4 cut(s)	1199, 1231, 1278, 1310
Asp700I	GAANNNNTTC	1 cut(s)	40
AspLEI	GCGC	3 cut(s)	342, 368, 438
AspS9I	GGNCC	5 cut(s)	115, 206, 665, 730, 2255
AsuC2I	CCSGG	2 cut(s)	811, 826
AsuII	TTCGAA	2 cut(s)	1372, 2077
AvaI	CYCGRG	2 cut(s)	326, 976
AvaII	GGWCC	4 cut(s)	115, 206, 730, 2255
BaeGI	GKGCMC	1 cut(s)	1942
BalI	TGGCCA	1 cut(s)	1277
BanII	GRGCYC	2 cut(s)	333, 549
BauI	CACGAG	1 cut(s)	1670
BbsI	GAAGAC	1 cut(s)	995
Bbv12I	GWGCWC	5 cut(s)	266, 333, 549, 2007, 2303
BbvCI	CCTCAGC	2 cut(s)	474, 1591
BbvI	GCAGC	6 cut(s)	574, 598, 602, 605, 749, 923
BceAI	ACGGC	4 cut(s)	594, 620, 1555, 2154
BcgI	CGANNNNNNTGC	4 cut(s)	593, 627, 1199, 1233
BciT130I	CCWGG	1 cut(s)	2174
BcnI	CCSGG	2 cut(s)	811, 826
BcoDI	GTCTC	6 cut(s)	569, 679, 1321, 1751, 2051, 2259
BfaI	CTAG	5 cut(s)	203, 671, 806, 1130, 1568
BfmI	CTRYAG	4 cut(s)	414, 588, 1943, 2061
BfoI	RGCGCY	1 cut(s)	369
BglI	GCCNNNNNGGC	2 cut(s)	357, 1652
BglII	AGATCT	1 cut(s)	183
BisI	GCNGC	8 cut(s)	352, 588, 591, 594, 612, 763, 912, 2142
BlsI	GCNGC	8 cut(s)	353, 589, 592, 595, 613, 764, 913, 2143
Bme1390I	CCNGG	3 cut(s)	811, 826, 2174
Bme18I	GGWCC	4 cut(s)	115, 206, 730, 2255
BmeT110I	CYCGRG	2 cut(s)	326, 976
BmgBI	CACGTC	1 cut(s)	607
BmgT120I	GGNCC	5 cut(s)	115, 206, 665, 730, 2255
BmiI	GGNNCC	6 cut(s)	6, 656, 1441, 1924, 2189, 2256
BmrFI	CCNGG	3 cut(s)	811, 826, 2174
BmrI	ACTGGG	1 cut(s)	2261
BmsI	GCATC	6 cut(s)	300, 543, 1464, 1486, 1656, 1786
BmuI	ACTGGG	1 cut(s)	2261
BpiI	GAAGAC	1 cut(s)	995
BpmI	CTGGAG	2 cut(s)	1804, 2258
Bpu10I	CCTNAGC	3 cut(s)	474, 643, 1591
Bpu14I	TTCGAA	2 cut(s)	1372, 2077
BpuMI	CCSGG	2 cut(s)	811, 826
BsaAI	YACGTR	1 cut(s)	1471
BsaJI	CCNNGG	4 cut(s)	168, 630, 636, 810
BsaWI	WCCGGW	1 cut(s)	1436
BsaXI	ACNNNNNCTCC	2 cut(s)	326, 356
Bsc4I	CCNNNNNNNGG	9 cut(s)	169, 810, 811, 864, 899, 1690, 1992, 2044, 2289
Bse118I	RCCGGY	1 cut(s)	647
Bse1I	ACTGG	3 cut(s)	1066, 1109, 2256
Bse3DI	GCAATG	3 cut(s)	174, 588, 1098
BseBI	CCWGG	1 cut(s)	2174
BseDI	CCNNGG	4 cut(s)	168, 630, 636, 810
BseGI	GGATG	3 cut(s)	1502, 1629, 2279
BseLI	CCNNNNNNNGG	9 cut(s)	169, 810, 811, 864, 899, 1690, 1992, 2044, 2289
BseMI	GCAATG	3 cut(s)	174, 588, 1098
BseMII	CTCAG	5 cut(s)	488, 1302, 1605, 1839, 1857
BseNI	ACTGG	3 cut(s)	1066, 1109, 2256
BseRI	GAGGAG	2 cut(s)	290, 470
BseSI	GKGCMC	1 cut(s)	1942
BseXI	GCAGC	6 cut(s)	574, 598, 602, 605, 749, 923
BseYI	CCCAGC	1 cut(s)	1340
BsgI	GTGCAG	1 cut(s)	198
Bsh1285I	CGRYCG	1 cut(s)	1437
BshFI	GGCC	9 cut(s)	222, 297, 324, 443, 629, 635, 641, 666, 1277
BsiEI	CGRYCG	1 cut(s)	1437
BsiHKAI	GWGCWC	5 cut(s)	266, 333, 549, 2007, 2303
BsiHKCI	CYCGRG	2 cut(s)	326, 976
BsiSI	CCGG	6 cut(s)	223, 648, 696, 810, 825, 1437
BslFI	GGGAC	1 cut(s)	2268
BslI	CCNNNNNNNGG	9 cut(s)	169, 810, 811, 864, 899, 1690, 1992, 2044, 2289
BsmAI	GTCTC	6 cut(s)	569, 679, 1321, 1751, 2051, 2259
BsmBI	CGTCTC	3 cut(s)	569, 1751, 2259
BsmFI	GGGAC	1 cut(s)	2268
BsmI	GAATGC	1 cut(s)	1905
BsnI	GGCC	9 cut(s)	222, 297, 324, 443, 629, 635, 641, 666, 1277
BsoBI	CYCGRG	2 cut(s)	326, 976
Bsp119I	TTCGAA	2 cut(s)	1372, 2077
Bsp1286I	GDGCHC	6 cut(s)	266, 333, 549, 1942, 2007, 2303
Bsp143I	GATC	8 cut(s)	183, 225, 930, 1014, 1495, 1843, 2047, 2178
BspANI	GGCC	9 cut(s)	222, 297, 324, 443, 629, 635, 641, 666, 1277
BspCNI	CTCAG	5 cut(s)	487, 1303, 1604, 1838, 1858
BspHI	TCATGA	2 cut(s)	831, 1254
BspLI	GGNNCC	6 cut(s)	6, 656, 1441, 1924, 2189, 2256
BspMAI	CTGCAG	3 cut(s)	592, 1947, 2065
BspPI	GGATC	5 cut(s)	233, 938, 1022, 1851, 2055
BspT104I	TTCGAA	2 cut(s)	1372, 2077
BsrDI	GCAATG	3 cut(s)	174, 588, 1098
BsrFI	RCCGGY	1 cut(s)	647
BsrI	ACTGG	3 cut(s)	1066, 1109, 2256
BssAI	RCCGGY	1 cut(s)	647
BssECI	CCNNGG	4 cut(s)	168, 630, 636, 810
BssMI	GATC	8 cut(s)	183, 225, 930, 1014, 1495, 1843, 2047, 2178
BssSI	CACGAG	1 cut(s)	1670
BssT1I	CCWWGG	2 cut(s)	168, 630
Bst2BI	CACGAG	1 cut(s)	1670
Bst2UI	CCWGG	1 cut(s)	2174
Bst4CI	ACNGT	2 cut(s)	452, 1987
Bst6I	CTCTTC	5 cut(s)	125, 308, 1461, 1749, 1889
BstAPI	GCANNNNNTGC	1 cut(s)	587
BstBAI	YACGTR	1 cut(s)	1471
BstBI	TTCGAA	2 cut(s)	1372, 2077
BstC8I	GCNNGC	7 cut(s)	289, 388, 532, 627, 1544, 1579, 2146
BstDEI	CTNAG	9 cut(s)	187, 474, 538, 643, 1311, 1591, 1825, 1866, 2021
BstDSI	CCRYGG	1 cut(s)	636
BstEII	GGTNACC	1 cut(s)	467
BstF5I	GGATG	3 cut(s)	1502, 1629, 2279
BstH2I	RGCGCY	1 cut(s)	369
BstHHI	GCGC	3 cut(s)	342, 368, 438
BstKTI	GATC	8 cut(s)	186, 228, 933, 1017, 1498, 1846, 2050, 2181
BstMAI	GTCTC	6 cut(s)	569, 679, 1321, 1751, 2051, 2259
BstMBI	GATC	8 cut(s)	183, 225, 930, 1014, 1495, 1843, 2047, 2178
BstMCI	CGRYCG	1 cut(s)	1437
BstNI	CCWGG	1 cut(s)	2174
BstPI	GGTNACC	1 cut(s)	467
BstSCI	CCNGG	3 cut(s)	809, 824, 2172
BstSFI	CTRYAG	4 cut(s)	414, 588, 1943, 2061
BstSLI	GKGCMC	1 cut(s)	1942
BstV1I	GCAGC	6 cut(s)	574, 598, 602, 605, 749, 923
BstV2I	GAAGAC	1 cut(s)	995
BstX2I	RGATCY	2 cut(s)	183, 2047
BstXI	CCANNNNNNTGG	1 cut(s)	1516
BstYI	RGATCY	2 cut(s)	183, 2047
BsuRI	GGCC	9 cut(s)	222, 297, 324, 443, 629, 635, 641, 666, 1277
BtgI	CCRYGG	1 cut(s)	636
BtgZI	GCGATG	2 cut(s)	394, 1763
BtrI	CACGTC	1 cut(s)	607
BtsCI	GGATG	3 cut(s)	1502, 1629, 2279
BtsI	GCAGTG	3 cut(s)	361, 1940, 2070
BtsIMutI	CAGTG	5 cut(s)	361, 1059, 1116, 1940, 2070
Cac8I	GCNNGC	7 cut(s)	289, 388, 532, 627, 1544, 1579, 2146
CciI	TCATGA	2 cut(s)	831, 1254
CfoI	GCGC	3 cut(s)	342, 368, 438
Cfr10I	RCCGGY	1 cut(s)	647
Cfr13I	GGNCC	5 cut(s)	115, 206, 665, 730, 2255
CseI	GACGC	1 cut(s)	2276
CsiI	ACCWGGT	1 cut(s)	2172
Csp6I	GTAC	3 cut(s)	1406, 1520, 1529
CviQI	GTAC	3 cut(s)	1406, 1520, 1529
DdeI	CTNAG	9 cut(s)	187, 474, 538, 643, 1311, 1591, 1825, 1866, 2021
DpnI	GATC	8 cut(s)	185, 227, 932, 1016, 1497, 1845, 2049, 2180
DpnII	GATC	8 cut(s)	183, 225, 930, 1014, 1495, 1843, 2047, 2178
EaeI	YGGCCR	3 cut(s)	220, 633, 1275
Eam1104I	CTCTTC	5 cut(s)	125, 308, 1461, 1749, 1889
EarI	CTCTTC	5 cut(s)	125, 308, 1461, 1749, 1889
EciI	GGCGGA	1 cut(s)	149
Ecl136II	GAGCTC	2 cut(s)	331, 547
Eco130I	CCWWGG	2 cut(s)	168, 630
Eco147I	AGGCCT	1 cut(s)	324
Eco24I	GRGCYC	2 cut(s)	333, 549
Eco47I	GGWCC	4 cut(s)	115, 206, 730, 2255
Eco53kI	GAGCTC	2 cut(s)	331, 547
Eco57I	CTGAAG	2 cut(s)	27, 1913
Eco88I	CYCGRG	2 cut(s)	326, 976
Eco91I	GGTNACC	1 cut(s)	467
EcoICRI	GAGCTC	2 cut(s)	331, 547
EcoO109I	RGGNCCY	2 cut(s)	206, 665
EcoO65I	GGTNACC	1 cut(s)	467
EcoRI	GAATTC	4 cut(s)	905, 1333, 1450, 2099
EcoRII	CCWGG	1 cut(s)	2172
EcoT14I	CCWWGG	2 cut(s)	168, 630
EcoT22I	ATGCAT	1 cut(s)	1479
EcoT38I	GRGCYC	2 cut(s)	333, 549
ErhI	CCWWGG	2 cut(s)	168, 630
Esp3I	CGTCTC	3 cut(s)	569, 1751, 2259
FaqI	GGGAC	1 cut(s)	2268
FauI	CCCGC	2 cut(s)	1948, 2137
FblI	GTMKAC	2 cut(s)	1433, 1661
Fnu4HI	GCNGC	8 cut(s)	352, 588, 591, 594, 612, 763, 912, 2142
FokI	GGATG	3 cut(s)	1489, 1616, 2266
FriOI	GRGCYC	2 cut(s)	333, 549
Fsp4HI	GCNGC	8 cut(s)	352, 588, 591, 594, 612, 763, 912, 2142
FspBI	CTAG	5 cut(s)	203, 671, 806, 1130, 1568
FspI	TGCGCA	1 cut(s)	437
GlaI	GCGC	3 cut(s)	341, 367, 437
GluI	GCNGC	8 cut(s)	352, 588, 591, 594, 612, 763, 912, 2142
GsaI	CCCAGC	1 cut(s)	1344
GsuI	CTGGAG	2 cut(s)	1804, 2258
HaeII	RGCGCY	1 cut(s)	369
HaeIII	GGCC	9 cut(s)	222, 297, 324, 443, 629, 635, 641, 666, 1277
HapII	CCGG	6 cut(s)	223, 648, 696, 810, 825, 1437
HgaI	GACGC	1 cut(s)	2276
HhaI	GCGC	3 cut(s)	342, 368, 438
Hin6I	GCGC	3 cut(s)	340, 366, 436
HinP1I	GCGC	3 cut(s)	340, 366, 436
HincII	GTYRAC	1 cut(s)	1434
HindII	GTYRAC	1 cut(s)	1434
HindIII	AAGCTT	2 cut(s)	786, 1602
HinfI	GANTC	8 cut(s)	278, 420, 619, 1118, 1198, 1374, 1430, 1851
HpaII	CCGG	6 cut(s)	223, 648, 696, 810, 825, 1437
Hpy166II	GTNNAC	6 cut(s)	1406, 1434, 1468, 1522, 1662, 1850
Hpy188I	TCNGA	8 cut(s)	18, 46, 75, 894, 1312, 1447, 1893, 1971
Hpy188III	TCNNGA	5 cut(s)	832, 1138, 1174, 1255, 2051
Hpy8I	GTNNAC	6 cut(s)	1406, 1434, 1468, 1522, 1662, 1850
Hpy99I	CGWCG	2 cut(s)	608, 1211
HpyCH4III	ACNGT	2 cut(s)	452, 1987
HpyCH4IV	ACGT	4 cut(s)	606, 996, 1079, 1470
HpyF3I	CTNAG	9 cut(s)	187, 474, 538, 643, 1311, 1591, 1825, 1866, 2021
HpySE526I	ACGT	4 cut(s)	606, 996, 1079, 1470
HspAI	GCGC	3 cut(s)	340, 366, 436
Kzo9I	GATC	8 cut(s)	183, 225, 930, 1014, 1495, 1843, 2047, 2178
LmnI	GCTCC	9 cut(s)	50, 261, 347, 483, 1162, 1862, 2002, 2239, 2306
Lsp1109I	GCAGC	6 cut(s)	574, 598, 602, 605, 749, 923
LweI	GCATC	6 cut(s)	300, 543, 1464, 1486, 1656, 1786
MabI	ACCWGGT	1 cut(s)	2172
MaeI	CTAG	5 cut(s)	203, 671, 806, 1130, 1568
MaeII	ACGT	4 cut(s)	606, 996, 1079, 1470
MaeIII	GTNAC	6 cut(s)	421, 467, 997, 1624, 1634, 1981
MalI	GATC	8 cut(s)	185, 227, 932, 1016, 1497, 1845, 2049, 2180
MboI	GATC	8 cut(s)	183, 225, 930, 1014, 1495, 1843, 2047, 2178
MboII	GAAGA	9 cut(s)	33, 112, 127, 295, 1000, 1448, 1766, 1906, 1906
MfeI	CAATTG	2 cut(s)	2208, 2259
MflI	RGATCY	2 cut(s)	183, 2047
MhlI	GDGCHC	6 cut(s)	266, 333, 549, 1942, 2007, 2303
MlsI	TGGCCA	1 cut(s)	1277
MluNI	TGGCCA	1 cut(s)	1277
MlyI	GAGTC	4 cut(s)	429, 1127, 1439, 1845
MmeI	TCCRAC	1 cut(s)	1840
Mox20I	TGGCCA	1 cut(s)	1277
Mph1103I	ATGCAT	1 cut(s)	1479
MroXI	GAANNNNTTC	1 cut(s)	40
MscI	TGGCCA	1 cut(s)	1277
MseI	TTAA	3 cut(s)	800, 884, 2154
MslI	CAYNNNNRTG	2 cut(s)	1253, 1514
Msp20I	TGGCCA	1 cut(s)	1277
MspA1I	CMGCKG	2 cut(s)	762, 2236
MspI	CCGG	6 cut(s)	223, 648, 696, 810, 825, 1437
MspR9I	CCNGG	3 cut(s)	811, 826, 2174
MunI	CAATTG	2 cut(s)	2208, 2259
Mva1269I	GAATGC	1 cut(s)	1905
MvaI	CCWGG	1 cut(s)	2174
NciI	CCSGG	2 cut(s)	811, 826
NdeII	GATC	8 cut(s)	183, 225, 930, 1014, 1495, 1843, 2047, 2178
NlaIV	GGNNCC	6 cut(s)	6, 656, 1441, 1924, 2189, 2256
NmeAIII	GCCGAG	2 cut(s)	205, 1631
NmuCI	GTSAC	3 cut(s)	421, 1624, 1634
NsbI	TGCGCA	1 cut(s)	437
NsiI	ATGCAT	1 cut(s)	1479
NspV	TTCGAA	2 cut(s)	1372, 2077
PaeR7I	CTCGAG	2 cut(s)	326, 976
PagI	TCATGA	2 cut(s)	831, 1254
PceI	AGGCCT	1 cut(s)	324
PcsI	WCGNNNNNNNCGW	2 cut(s)	324, 1923
PctI	GAATGC	1 cut(s)	1905
PdmI	GAANNNNTTC	1 cut(s)	40
PfeI	GAWTC	4 cut(s)	278, 619, 1198, 1374
PkrI	GCNGC	8 cut(s)	353, 589, 592, 595, 613, 764, 913, 2143
PleI	GAGTC	4 cut(s)	428, 1126, 1438, 1845
PpsI	GAGTC	4 cut(s)	428, 1126, 1438, 1845
Ppu21I	YACGTR	1 cut(s)	1471
PpuMI	RGGWCCY	1 cut(s)	206
Psp124BI	GAGCTC	2 cut(s)	333, 549
Psp1406I	AACGTT	1 cut(s)	1079
Psp5II	RGGWCCY	1 cut(s)	206
Psp6I	CCWGG	1 cut(s)	2172
PspEI	GGTNACC	1 cut(s)	467
PspFI	CCCAGC	1 cut(s)	1340
PspGI	CCWGG	1 cut(s)	2172
PspN4I	GGNNCC	6 cut(s)	6, 656, 1441, 1924, 2189, 2256
PspPI	GGNCC	5 cut(s)	115, 206, 665, 730, 2255
PspPPI	RGGWCCY	1 cut(s)	206
PspXI	VCTCGAGB	2 cut(s)	326, 976
PstI	CTGCAG	3 cut(s)	592, 1947, 2065
PsuI	RGATCY	2 cut(s)	183, 2047
PvuII	CAGCTG	1 cut(s)	2236
RsaI	GTAC	3 cut(s)	1407, 1521, 1530
RsaNI	GTAC	3 cut(s)	1406, 1520, 1529
RseI	CAYNNNNRTG	2 cut(s)	1253, 1514
SacI	GAGCTC	2 cut(s)	333, 549
SalI	GTCGAC	1 cut(s)	1432
SaqAI	TTAA	3 cut(s)	800, 884, 2154
SatI	GCNGC	8 cut(s)	352, 588, 591, 594, 612, 763, 912, 2142
Sau3AI	GATC	8 cut(s)	183, 225, 930, 1014, 1495, 1843, 2047, 2178
Sau96I	GGNCC	5 cut(s)	115, 206, 665, 730, 2255
SchI	GAGTC	4 cut(s)	429, 1127, 1439, 1845
ScrFI	CCNGG	3 cut(s)	811, 826, 2174
SduI	GDGCHC	6 cut(s)	266, 333, 549, 1942, 2007, 2303
SexAI	ACCWGGT	1 cut(s)	2172
SfaNI	GCATC	6 cut(s)	300, 543, 1464, 1486, 1656, 1786
SfcI	CTRYAG	4 cut(s)	414, 588, 1943, 2061
Sfr274I	CTCGAG	2 cut(s)	326, 976
SfuI	TTCGAA	2 cut(s)	1372, 2077
SinI	GGWCC	4 cut(s)	115, 206, 730, 2255
SlaI	CTCGAG	2 cut(s)	326, 976
SmiMI	CAYNNNNRTG	2 cut(s)	1253, 1514
SmlI	CTYRAG	2 cut(s)	326, 976
SmoI	CTYRAG	2 cut(s)	326, 976
SseBI	AGGCCT	1 cut(s)	324
SspI	AATATT	1 cut(s)	1180
SspMI	CTAG	5 cut(s)	203, 671, 806, 1130, 1568
SstI	GAGCTC	2 cut(s)	333, 549
StuI	AGGCCT	1 cut(s)	324
StyD4I	CCNGG	3 cut(s)	809, 824, 2172
StyI	CCWWGG	2 cut(s)	168, 630
TaaI	ACNGT	2 cut(s)	452, 1987
TaiI	ACGT	4 cut(s)	609, 999, 1082, 1473
TaqI	TCGA	9 cut(s)	276, 327, 333, 933, 977, 1352, 1372, 1433, 2077
TauI	GCSGC	2 cut(s)	354, 2144
TfiI	GAWTC	4 cut(s)	278, 619, 1198, 1374
Tru1I	TTAA	3 cut(s)	800, 884, 2154
Tru9I	TTAA	3 cut(s)	800, 884, 2154
TscAI	CASTG	5 cut(s)	361, 1066, 1116, 1947, 2070
TseFI	GTSAC	3 cut(s)	421, 1624, 1634
TseI	GCWGC	6 cut(s)	587, 590, 593, 611, 762, 911
Tsp45I	GTSAC	3 cut(s)	421, 1624, 1634
TspGWI	ACGGA	4 cut(s)	722, 1546, 1622, 2293
TspRI	CASTG	5 cut(s)	361, 1066, 1116, 1947, 2070
VpaK11BI	GGWCC	4 cut(s)	115, 206, 730, 2255
XapI	RAATTY	5 cut(s)	905, 1068, 1333, 1450, 2099
XcmI	CCANNNNNNNNNTGG	1 cut(s)	860
XhoI	CTCGAG	2 cut(s)	326, 976
XmiI	GTMKAC	2 cut(s)	1433, 1661
XmnI	GAANNNNTTC	1 cut(s)	40
XspI	CTAG	5 cut(s)	203, 671, 806, 1130, 1568
Zsp2I	ATGCAT	1 cut(s)	1479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.