MD12G1039000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017334
Physical Location & Seq
Reverse (-)
4224129 .. 4224791
663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1039000.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGCTTCTTTGTGGTGCAAATCATCTCCATATTTCAATAAAGCTCGTGGATACATAGTCCTTGTTTTGATTGTGGTGAGTCTAGCAACCGCCATCGGATTTTCTTTATGGTTCGTTGGCAACTATGTGGATTCAAAGTCCGGTCCGGGGACAGACCCCCTTGTGCTTCGTTTGGATGCATTTTCTCTATCGAATTTTGAAGTTTCAAATTCGTCACTTTCAGCTGAGTGGGAAGCCAAGTTGACATTTGGGAACCAAAATGGCGGCTTAATTGTTACCCTCAGCCCTTTTGCGAGTTATGTGTATTACAAAGAAAGTGAGGCTCTATCATGTGCTTCAGTGGATGCAACGCTGCGCATTCCTCCTAGGAAGCAGAAAACTGTACAGATCAAGTTTGATTCCACCAGTTGTGAGGGGGAACAACCGTATGTGGAAGATCGAGTGATGAAGCAGTTGTGTGAAGATAAAAAGAAGGGTTACCTTAGTTTTAGTTTGAAGATGCATATAGATGCTTCCTATAGTATGAAGGAGCTACTGGGAATGGGAACCCAAGTGGCCTTAAATCCCAACTGTTCAGGTTTGAAGGTTGAGTTTTGGGGTTCAAAAGGTACCGAGGGAAAGATAATTGAGGGGCGTAAGTGCTCCATTCCGTTGCCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

24.21

Weight (kDa)

8.65

Isoelectric Point (pI)

31.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017334)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20210
malus_domestica MD12G1039000.v1.1
prunus_persica Prupe.7G096800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475751
rosa_laevigata RLG00000023819
rosa_multiflora Rmu_sc0000539.1_g000074
rosa_roxburghii Rroxscaffold_6G00405890
rosa_rugosa Rorug03G0151200
rosa_samantha Rh3AG200900 Rh3BG231300 Rh3CG226000 Rh3DG226500
rosa_wichuraiana Rw3G018280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 356
Acc65I GGTACC 1 cut(s) 608
AccB1I GGYRCC 1 cut(s) 608
AciI CCGC 2 cut(s) 90, 264
AcsI RAATTY 2 cut(s) 193, 208
AcuI CTGAAG 1 cut(s) 321
AfaI GTAC 2 cut(s) 384, 610
AfiI CCNNNNNNNGG 1 cut(s) 147
AgsI TTSAA 7 cut(s) 37, 135, 200, 207, 496, 583, 603
AluBI AGCT 3 cut(s) 44, 224, 532
AluI AGCT 3 cut(s) 44, 224, 532
Alw21I GWGCWC 1 cut(s) 644
AoxI GGCC 1 cut(s) 555
ApeKI GCWGC 1 cut(s) 352
ApoI RAATTY 2 cut(s) 193, 208
Asp718I GGTACC 1 cut(s) 608
AspA2I CCTAGG 1 cut(s) 365
AspLEI GCGC 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 88
AvaII GGWCC 1 cut(s) 143
AvrII CCTAGG 1 cut(s) 365
BanI GGYRCC 1 cut(s) 608
BauI CACGAG 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 644
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 1 cut(s) 339
BccI CCATC 1 cut(s) 101
BciVI GTATCC 1 cut(s) 44
BcnI CCSGG 1 cut(s) 147
BfaI CTAG 2 cut(s) 83, 366
BfmI CTRYAG 1 cut(s) 517
BfuI GTATCC 1 cut(s) 44
BisI GCNGC 2 cut(s) 265, 353
BlnI CCTAGG 1 cut(s) 365
BlsI GCNGC 2 cut(s) 266, 354
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 3 cut(s) 254, 547, 610
BmrFI CCNGG 1 cut(s) 147
BmrI ACTGGG 1 cut(s) 545
BmsI GCATC 4 cut(s) 166, 334, 489, 499
BmuI ACTGGG 1 cut(s) 545
Bpu10I CCTNAGC 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 147
BsaJI CCNNGG 3 cut(s) 146, 365, 612
BsaWI WCCGGW 1 cut(s) 140
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 2 cut(s) 405, 540
BseDI CCNNGG 3 cut(s) 146, 365, 612
BseGI GGATG 2 cut(s) 181, 349
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMII CTCAG 2 cut(s) 216, 295
BseNI ACTGG 2 cut(s) 405, 540
BseXI GCAGC 1 cut(s) 339
BshFI GGCC 1 cut(s) 557
BshNI GGYRCC 1 cut(s) 608
BsiHKAI GWGCWC 1 cut(s) 644
BsiSI CCGG 2 cut(s) 141, 146
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 163
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 644
Bsp1407I TGTACA 1 cut(s) 382
Bsp143I GATC 2 cut(s) 387, 436
BspACI CCGC 2 cut(s) 90, 264
BspANI GGCC 1 cut(s) 557
BspCNI CTCAG 2 cut(s) 217, 294
BspLI GGNNCC 3 cut(s) 254, 547, 610
BspT107I GGYRCC 1 cut(s) 608
BsrGI TGTACA 1 cut(s) 382
BsrI ACTGG 2 cut(s) 405, 540
BssECI CCNNGG 3 cut(s) 146, 365, 612
BssMI GATC 2 cut(s) 387, 436
BssSI CACGAG 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 45
Bst4CI ACNGT 3 cut(s) 382, 426, 572
BstAUI TGTACA 1 cut(s) 382
BstDEI CTNAG 3 cut(s) 225, 281, 482
BstEII GGTNACC 1 cut(s) 476
BstF5I GGATG 2 cut(s) 181, 349
BstHHI GCGC 1 cut(s) 357
BstKTI GATC 2 cut(s) 390, 439
BstMBI GATC 2 cut(s) 387, 436
BstPI GGTNACC 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 1 cut(s) 517
BstV1I GCAGC 1 cut(s) 339
BsuI GTATCC 1 cut(s) 44
BsuRI GGCC 1 cut(s) 557
BtsCI GGATG 2 cut(s) 181, 349
BtsIMutI CAGTG 1 cut(s) 345
CfoI GCGC 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 143
CpoI CGGWCCG 1 cut(s) 143
Csp6I GTAC 2 cut(s) 383, 609
CspI CGGWCCG 1 cut(s) 143
CviAII CATG 1 cut(s) 330
CviJI RGCY 9 cut(s) 5, 44, 224, 236, 267, 285, 323, 532, 557
CviKI_1 RGCY 9 cut(s) 5, 44, 224, 236, 267, 285, 323, 532, 557
CviQI GTAC 2 cut(s) 383, 609
DdeI CTNAG 3 cut(s) 225, 281, 482
DpnI GATC 2 cut(s) 389, 438
DpnII GATC 2 cut(s) 387, 436
Eco130I CCWWGG 1 cut(s) 365
Eco47I GGWCC 1 cut(s) 143
Eco57I CTGAAG 1 cut(s) 321
Eco91I GGTNACC 1 cut(s) 476
EcoO65I GGTNACC 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 365
EcoT22I ATGCAT 2 cut(s) 181, 504
ErhI CCWWGG 1 cut(s) 365
FaeI CATG 1 cut(s) 333
FaqI GGGAC 1 cut(s) 163
FatI CATG 1 cut(s) 329
Fnu4HI GCNGC 2 cut(s) 265, 353
FokI GGATG 2 cut(s) 188, 356
Fsp4HI GCNGC 2 cut(s) 265, 353
FspBI CTAG 2 cut(s) 83, 366
FspI TGCGCA 1 cut(s) 356
GlaI GCGC 1 cut(s) 356
GluI GCNGC 2 cut(s) 265, 353
HaeIII GGCC 1 cut(s) 557
HapII CCGG 2 cut(s) 141, 146
HhaI GCGC 1 cut(s) 357
Hin1II CATG 1 cut(s) 333
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HincII GTYRAC 1 cut(s) 243
HindII GTYRAC 1 cut(s) 243
HinfI GANTC 3 cut(s) 79, 131, 398
HpaII CCGG 2 cut(s) 141, 146
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 1 cut(s) 243
Hpy188I TCNGA 1 cut(s) 98
Hpy8I GTNNAC 1 cut(s) 243
HpyAV CCTTC 3 cut(s) 466, 520, 577
HpyCH4III ACNGT 3 cut(s) 382, 426, 572
HpyCH4V TGCA 4 cut(s) 18, 179, 347, 502
HpyF3I CTNAG 3 cut(s) 225, 281, 482
Hsp92II CATG 1 cut(s) 333
HspAI GCGC 1 cut(s) 355
KpnI GGTACC 1 cut(s) 612
Kzo9I GATC 2 cut(s) 387, 436
LmnI GCTCC 2 cut(s) 529, 647
LpnPI CCDG 5 cut(s) 154, 159, 418, 521, 561
Lsp1109I GCAGC 1 cut(s) 339
LweI GCATC 4 cut(s) 166, 334, 489, 499
MaeI CTAG 2 cut(s) 83, 366
MaeIII GTNAC 3 cut(s) 213, 274, 476
MalI GATC 2 cut(s) 389, 438
MboI GATC 2 cut(s) 387, 436
MboII GAAGA 3 cut(s) 446, 473, 508
MhlI GDGCHC 1 cut(s) 644
MluCI AATT 4 cut(s) 193, 208, 270, 624
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 6 cut(s) 290, 313, 372, 406, 607, 622
Mph1103I ATGCAT 2 cut(s) 181, 504
MseI TTAA 2 cut(s) 269, 560
MslI CAYNNNNRTG 1 cut(s) 507
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 2 cut(s) 141, 146
MspR9I CCNGG 1 cut(s) 147
Mva1269I GAATGC 1 cut(s) 357
NciI CCSGG 1 cut(s) 147
NdeII GATC 2 cut(s) 387, 436
NlaIII CATG 1 cut(s) 333
NlaIV GGNNCC 3 cut(s) 254, 547, 610
NmuCI GTSAC 1 cut(s) 213
NsbI TGCGCA 1 cut(s) 356
NsiI ATGCAT 2 cut(s) 181, 504
PctI GAATGC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 131, 398
PkrI GCNGC 2 cut(s) 266, 354
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PspEI GGTNACC 1 cut(s) 476
PspN4I GGNNCC 3 cut(s) 254, 547, 610
PspPI GGNCC 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 2 cut(s) 384, 610
RsaNI GTAC 2 cut(s) 383, 609
RseI CAYNNNNRTG 1 cut(s) 507
Rsr2I CGGWCCG 1 cut(s) 143
RsrII CGGWCCG 1 cut(s) 143
SaqAI TTAA 2 cut(s) 269, 560
SatI GCNGC 2 cut(s) 265, 353
Sau3AI GATC 2 cut(s) 387, 436
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 644
SetI ASST 7 cut(s) 46, 226, 483, 534, 580, 588, 610
SfaNI GCATC 4 cut(s) 166, 334, 489, 499
SfcI CTRYAG 1 cut(s) 517
SinI GGWCC 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 507
Sse9I AATT 4 cut(s) 193, 208, 270, 624
SsiI CCGC 2 cut(s) 90, 264
SspMI CTAG 2 cut(s) 83, 366
StyD4I CCNGG 1 cut(s) 145
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 3 cut(s) 382, 426, 572
TaqI TCGA 2 cut(s) 191, 439
TasI AATT 4 cut(s) 193, 208, 270, 624
TatI WGTACW 1 cut(s) 382
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 2 cut(s) 131, 398
Tru1I TTAA 2 cut(s) 269, 560
Tru9I TTAA 2 cut(s) 269, 560
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 352
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 2 cut(s) 461, 539
TspGWI ACGGA 1 cut(s) 639
TspRI CASTG 1 cut(s) 345
VpaK11BI GGWCC 1 cut(s) 143
XapI RAATTY 2 cut(s) 193, 208
XmaJI CCTAGG 1 cut(s) 365
XspI CTAG 2 cut(s) 83, 366
Zsp2I ATGCAT 2 cut(s) 181, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.