Rorug03G0151200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0017334
Physical Location & Seq
Reverse (-)
12434524 .. 12436643
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0151200.1

Sequence Viewer

Length: 1239 bp
ATGTTGGCCAAGAAATTGAAGCATGAGGAGAGTTCAATGGAAGGATCATCATGTCAAATGACTACTGGATGGAAATGTCATCAAAGGGAAATAATTGCTGCACATATTTTGGTACAGATGGCTAATGAAGATTCTGGTTTGCTTCGTCACGGGTTAAATTCGCATGGAGTGACGCATAGCATGAAAGGGATGCTGAGAATGAGGTGGGAAACGAGAGGTGGTAGAATAGCAGGTGATAGTCATTCTGAAGTGGATGGGAGAAAGGCCAAGTTTCTGGCAAAGGGCCAGCAAGCTGCCTTGGGAGGTCAGATGAATGATGGAGATAGTGTAGTCTTGGCTTTATCAGCAATGCCATGTCCTGGAAGGAGAAAGCATACAAAGACGAACATCAGTATGGAACTATCTTCTCTTCGACCAAAGTATATGAACTGTAATGTCAGTGAGGACAAGGATGACAATTTGTTTGAAGAGTGTGGAGTTTTTGATGGTGATGAGCTGACTAGTAGATGGGGAAACAATAATCAGAAATCAATTGAAGCTGGTCTCGAGGATCCTCATGATAGATTAGGCATGTCAAGGATAAAGAAGCGGAAAACAGTGGCAATATCCTGCTTTAGTAGAGAAACTGAGGATGTTCAAGAGAGACTTCGTCATGTTGGAAGCCATCCTGCTGCACCACCTCTGGTTTTGATGGAACCAACATTAAACAAAAGGGAGAGGTTTAGGGGTATGAAACGTAAGATGAACATCGCCACTATCAGCCCAAGCTCTGAAGAAGTAAGAGCTCACTTTCGCAAGGAGGAAGCATTGAGGTATTTAGTCTCGGATAGGGGATTTTGCTACACTGCTCTGGATGGTAGAAAGTCTGCAGTCGCTCCTCTAACAAAGATGAAAAAGATTGCCCGATATCATGTTATACTCAAACCTGATAGGCCGCGTCATATTTACACCCTGTGTCTGGTGCGGGATGCAGCTGCTAGATTGCCTGGAGGTGTGGGCACCAGAGCAGATATTTGTACACTCATGCGCGACTCACAGTATCTAGTTGAAGACATCTCCGATAAACAACTTAATCAAGTTGCTAGCAAAGGACTGGACAGGCTGCACTACGAAGTTGATCCATGTGTTCAATATAATGGGAAGGTGTGGGTTTATTTACATCGAAATAGAGGGGAGGACGACTTTAAGGACGATGCTACATCATCTGCCTATGTAAGAAGAAGGTACCGCGGAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

412

Amino Acids

46.61

Weight (kDa)

9.39

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_2nd_NFRKB PF25793 261 - 393 5e-52 NFRKB second winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017334)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20210
malus_domestica MD12G1039000.v1.1
prunus_persica Prupe.7G096800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475751
rosa_laevigata RLG00000023819
rosa_multiflora Rmu_sc0000539.1_g000074
rosa_roxburghii Rroxscaffold_6G00405890
rosa_rugosa Rorug03G0151200
rosa_samantha Rh3AG200900 Rh3BG231300 Rh3CG226000 Rh3DG226500
rosa_wichuraiana Rw3G018280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 221
Acc65I GGTACC 1 cut(s) 1224
AccB1I GGYRCC 2 cut(s) 998, 1224
AccB7I CCANNNNNTGG 1 cut(s) 359
AccII CGCG 3 cut(s) 937, 1029, 1230
AciI CCGC 5 cut(s) 589, 935, 964, 1228, 1230
AclWI GGATC 4 cut(s) 52, 545, 558, 1112
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 1 cut(s) 157
AcuI CTGAAG 2 cut(s) 267, 792
AdeI CACNNNGTG 1 cut(s) 954
AfaI GTAC 3 cut(s) 114, 1018, 1226
AfiI CCNNNNNNNGG 2 cut(s) 359, 958
AgsI TTSAA 7 cut(s) 19, 36, 467, 536, 638, 1049, 1130
AhlI ACTAGT 1 cut(s) 500
AjnI CCWGG 2 cut(s) 358, 985
AluBI AGCT 6 cut(s) 293, 496, 539, 768, 785, 974
AluI AGCT 6 cut(s) 293, 496, 539, 768, 785, 974
Alw21I GWGCWC 1 cut(s) 787
Alw26I GTCTC 3 cut(s) 548, 637, 826
AlwI GGATC 4 cut(s) 52, 545, 558, 1112
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 4 cut(s) 6, 264, 283, 932
ApeKI GCWGC 6 cut(s) 98, 293, 671, 971, 974, 1102
ApoI RAATTY 1 cut(s) 157
ArsI GACNNNNNNTTYG 2 cut(s) 446, 478
Asp718I GGTACC 1 cut(s) 1224
AspLEI GCGC 1 cut(s) 1029
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 2 cut(s) 245, 500
AsuNHI GCTAGC 1 cut(s) 1082
AvaI CYCGRG 1 cut(s) 545
BaeGI GKGCMC 1 cut(s) 1001
BalI TGGCCA 1 cut(s) 8
BamHI GGATCC 1 cut(s) 550
BanI GGYRCC 2 cut(s) 998, 1224
BanII GRGCYC 1 cut(s) 787
BbsI GAAGAC 1 cut(s) 1056
Bbv12I GWGCWC 1 cut(s) 787
BbvI GCAGC 6 cut(s) 85, 280, 658, 961, 983, 1089
BccI CCATC 9 cut(s) 63, 112, 248, 311, 479, 501, 672, 685, 848
BciT130I CCWGG 2 cut(s) 360, 987
BcoDI GTCTC 3 cut(s) 548, 637, 826
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 4 cut(s) 501, 978, 1043, 1083
BfmI CTRYAG 1 cut(s) 867
BfuAI ACCTGC 1 cut(s) 221
BisI GCNGC 7 cut(s) 99, 294, 672, 935, 972, 975, 1103
BlsI GCNGC 7 cut(s) 100, 295, 673, 936, 973, 976, 1104
Bme1390I CCNGG 2 cut(s) 360, 987
BmeT110I CYCGRG 1 cut(s) 545
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 4 cut(s) 552, 696, 1000, 1226
BmrFI CCNGG 2 cut(s) 360, 987
BmsI GCATC 3 cut(s) 180, 958, 1183
BmtI GCTAGC 1 cut(s) 1086
BpiI GAAGAC 1 cut(s) 1056
BpmI CTGGAG 1 cut(s) 1008
BsaBI GATNNNNATC 1 cut(s) 746
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 2 cut(s) 297, 1228
BsaXI ACNNNNNCTCC 2 cut(s) 312, 342
Bsc4I CCNNNNNNNGG 2 cut(s) 359, 958
Bse1I ACTGG 2 cut(s) 70, 1098
Bse3DI GCAATG 1 cut(s) 354
Bse8I GATNNNNATC 1 cut(s) 746
BseBI CCWGG 2 cut(s) 360, 987
BseDI CCNNGG 2 cut(s) 297, 1228
BseGI GGATG 8 cut(s) 74, 195, 259, 457, 637, 664, 859, 973
BseJI GATNNNNATC 1 cut(s) 746
BseLI CCNNNNNNNGG 2 cut(s) 359, 958
BseMI GCAATG 1 cut(s) 354
BseMII CTCAG 2 cut(s) 185, 618
BseNI ACTGG 2 cut(s) 70, 1098
BseRI GAGGAG 2 cut(s) 41, 867
BseSI GKGCMC 1 cut(s) 1001
BseXI GCAGC 6 cut(s) 85, 280, 658, 961, 983, 1089
BsgI GTGCAG 3 cut(s) 84, 657, 1088
Bsh1236I CGCG 3 cut(s) 937, 1029, 1230
BshFI GGCC 4 cut(s) 8, 266, 285, 934
BshNI GGYRCC 2 cut(s) 998, 1224
BsiHKAI GWGCWC 1 cut(s) 787
BsiHKCI CYCGRG 1 cut(s) 545
BslI CCNNNNNNNGG 2 cut(s) 359, 958
BsmAI GTCTC 3 cut(s) 548, 637, 826
BsnI GGCC 4 cut(s) 8, 266, 285, 934
Bso31I GGTCTC 1 cut(s) 548
BsoBI CYCGRG 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 787, 1001
Bsp1407I TGTACA 1 cut(s) 1016
Bsp143I GATC 3 cut(s) 44, 550, 1117
BspACI CCGC 5 cut(s) 589, 935, 964, 1228, 1230
BspANI GGCC 4 cut(s) 8, 266, 285, 934
BspCNI CTCAG 2 cut(s) 186, 619
BspFNI CGCG 3 cut(s) 937, 1029, 1230
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 4 cut(s) 552, 696, 1000, 1226
BspMAI CTGCAG 1 cut(s) 871
BspMI ACCTGC 1 cut(s) 221
BspOI GCTAGC 1 cut(s) 1086
BspPI GGATC 4 cut(s) 52, 545, 558, 1112
BspT107I GGYRCC 2 cut(s) 998, 1224
BspTNI GGTCTC 1 cut(s) 548
BsrDI GCAATG 1 cut(s) 354
BsrGI TGTACA 1 cut(s) 1016
BsrI ACTGG 2 cut(s) 70, 1098
BssECI CCNNGG 2 cut(s) 297, 1228
BssMI GATC 3 cut(s) 44, 550, 1117
BssT1I CCWWGG 1 cut(s) 297
Bst2UI CCWGG 2 cut(s) 360, 987
Bst4CI ACNGT 3 cut(s) 431, 598, 1038
Bst6I CTCTTC 2 cut(s) 414, 462
BstAUI TGTACA 1 cut(s) 1016
BstC8I GCNNGC 3 cut(s) 287, 291, 1084
BstDEI CTNAG 2 cut(s) 194, 627
BstDSI CCRYGG 1 cut(s) 1228
BstF5I GGATG 8 cut(s) 74, 195, 259, 457, 637, 664, 859, 973
BstFNI CGCG 3 cut(s) 937, 1029, 1230
BstHHI GCGC 1 cut(s) 1029
BstKTI GATC 3 cut(s) 47, 553, 1120
BstMAI GTCTC 3 cut(s) 548, 637, 826
BstMBI GATC 3 cut(s) 44, 550, 1117
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 2 cut(s) 360, 987
BstNSI RCATGY 1 cut(s) 574
BstSCI CCNGG 2 cut(s) 358, 985
BstSFI CTRYAG 1 cut(s) 867
BstSLI GKGCMC 1 cut(s) 1001
BstUI CGCG 3 cut(s) 937, 1029, 1230
BstV1I GCAGC 6 cut(s) 85, 280, 658, 961, 983, 1089
BstV2I GAAGAC 1 cut(s) 1056
BstX2I RGATCY 1 cut(s) 550
BstXI CCANNNNNNTGG 1 cut(s) 274
BstYI RGATCY 1 cut(s) 550
BsuRI GGCC 4 cut(s) 8, 266, 285, 934
BtgI CCRYGG 1 cut(s) 1228
BtgZI GCGATG 1 cut(s) 733
BtsCI GGATG 8 cut(s) 74, 195, 259, 457, 637, 664, 859, 973
BtsI GCAGTG 1 cut(s) 843
BtsIMutI CAGTG 3 cut(s) 445, 603, 843
BveI ACCTGC 1 cut(s) 221
Cac8I GCNNGC 3 cut(s) 287, 291, 1084
CciI TCATGA 1 cut(s) 556
CfoI GCGC 1 cut(s) 1029
Cfr13I GGNCC 1 cut(s) 283
Cfr42I CCGCGG 1 cut(s) 1231
CseI GACGC 2 cut(s) 181, 926
Csp6I GTAC 3 cut(s) 113, 1017, 1225
CviQI GTAC 3 cut(s) 113, 1017, 1225
DdeI CTNAG 2 cut(s) 194, 627
DpnI GATC 3 cut(s) 46, 552, 1119
DpnII GATC 3 cut(s) 44, 550, 1117
DraIII CACNNNGTG 1 cut(s) 954
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 2 cut(s) 414, 462
EarI CTCTTC 2 cut(s) 414, 462
Ecl136II GAGCTC 1 cut(s) 785
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 1 cut(s) 787
Eco31I GGTCTC 1 cut(s) 548
Eco32I GATATC 1 cut(s) 908
Eco53kI GAGCTC 1 cut(s) 785
Eco57I CTGAAG 2 cut(s) 267, 792
Eco88I CYCGRG 1 cut(s) 545
EcoICRI GAGCTC 1 cut(s) 785
EcoRII CCWGG 2 cut(s) 358, 985
EcoRV GATATC 1 cut(s) 908
EcoT14I CCWWGG 1 cut(s) 297
EcoT38I GRGCYC 1 cut(s) 787
ErhI CCWWGG 1 cut(s) 297
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FauI CCCGC 1 cut(s) 957
Fnu4HI GCNGC 7 cut(s) 99, 294, 672, 935, 972, 975, 1103
FokI GGATG 8 cut(s) 81, 202, 266, 464, 644, 651, 866, 980
FriOI GRGCYC 1 cut(s) 787
Fsp4HI GCNGC 7 cut(s) 99, 294, 672, 935, 972, 975, 1103
FspBI CTAG 4 cut(s) 501, 978, 1043, 1083
GlaI GCGC 1 cut(s) 1028
GluI GCNGC 7 cut(s) 99, 294, 672, 935, 972, 975, 1103
GsuI CTGGAG 1 cut(s) 1008
HaeIII GGCC 4 cut(s) 8, 266, 285, 934
HgaI GACGC 2 cut(s) 181, 926
HhaI GCGC 1 cut(s) 1029
Hin6I GCGC 1 cut(s) 1027
HinP1I GCGC 1 cut(s) 1027
HinfI GANTC 2 cut(s) 131, 1031
HphI GGTGA 2 cut(s) 245, 500
Hpy166II GTNNAC 1 cut(s) 1019
Hpy188I TCNGA 6 cut(s) 247, 309, 525, 772, 826, 1060
Hpy188III TCNNGA 4 cut(s) 545, 557, 638, 851
Hpy8I GTNNAC 1 cut(s) 1019
HpyAV CCTTC 4 cut(s) 35, 357, 1135, 1215
HpyCH4III ACNGT 3 cut(s) 431, 598, 1038
HpyCH4IV ACGT 1 cut(s) 736
HpyCH4V TGCA 5 cut(s) 101, 674, 869, 971, 1105
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 2 cut(s) 194, 627
HpySE526I ACGT 1 cut(s) 736
HspAI GCGC 1 cut(s) 1027
KpnI GGTACC 1 cut(s) 1228
KspI CCGCGG 1 cut(s) 1231
Kzo9I GATC 3 cut(s) 44, 550, 1117
LmnI GCTCC 1 cut(s) 880
Lsp1109I GCAGC 6 cut(s) 85, 280, 658, 961, 983, 1089
LweI GCATC 3 cut(s) 180, 958, 1183
MaeI CTAG 4 cut(s) 501, 978, 1043, 1083
MaeII ACGT 1 cut(s) 736
MaeIII GTNAC 2 cut(s) 146, 169
MalI GATC 3 cut(s) 46, 552, 1119
MboI GATC 3 cut(s) 44, 550, 1117
MboII GAAGA 7 cut(s) 140, 396, 401, 479, 785, 1061, 1230
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 2 cut(s) 787, 1001
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 5 cut(s) 14, 93, 157, 457, 531
MluNI TGGCCA 1 cut(s) 8
MlyI GAGTC 1 cut(s) 1025
MmeI TCCRAC 1 cut(s) 637
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MseI TTAA 4 cut(s) 155, 704, 1071, 1185
MslI CAYNNNNRTG 1 cut(s) 392
Msp20I TGGCCA 1 cut(s) 8
MspA1I CMGCKG 2 cut(s) 974, 1230
MspR9I CCNGG 2 cut(s) 360, 987
MunI CAATTG 1 cut(s) 531
MvaI CCWGG 2 cut(s) 360, 987
MvnI CGCG 3 cut(s) 937, 1029, 1230
MwoI GCNNNNNNNGC 1 cut(s) 344
NdeII GATC 3 cut(s) 44, 550, 1117
NheI GCTAGC 1 cut(s) 1082
NlaIV GGNNCC 4 cut(s) 552, 696, 1000, 1226
NmuCI GTSAC 2 cut(s) 146, 169
NspI RCATGY 1 cut(s) 574
PaeR7I CTCGAG 1 cut(s) 545
PagI TCATGA 1 cut(s) 556
PaqCI CACCTGC 1 cut(s) 221
PfeI GAWTC 1 cut(s) 131
PflFI GACNNNGTC 1 cut(s) 648
PflMI CCANNNNNTGG 1 cut(s) 359
PfoI TCCNGGA 1 cut(s) 358
PkrI GCNGC 7 cut(s) 100, 295, 673, 936, 973, 976, 1104
PleI GAGTC 1 cut(s) 1025
PpsI GAGTC 1 cut(s) 1025
Psp124BI GAGCTC 1 cut(s) 787
Psp6I CCWGG 2 cut(s) 358, 985
PspGI CCWGG 2 cut(s) 358, 985
PspN4I GGNNCC 4 cut(s) 552, 696, 1000, 1226
PspPI GGNCC 1 cut(s) 283
PstI CTGCAG 1 cut(s) 871
PsuI RGATCY 1 cut(s) 550
PsyI GACNNNGTC 1 cut(s) 648
PvuII CAGCTG 1 cut(s) 974
RsaI GTAC 3 cut(s) 114, 1018, 1226
RsaNI GTAC 3 cut(s) 113, 1017, 1225
RseI CAYNNNNRTG 1 cut(s) 392
SacI GAGCTC 1 cut(s) 787
SacII CCGCGG 1 cut(s) 1231
SaqAI TTAA 4 cut(s) 155, 704, 1071, 1185
SatI GCNGC 7 cut(s) 99, 294, 672, 935, 972, 975, 1103
Sau3AI GATC 3 cut(s) 44, 550, 1117
Sau96I GGNCC 1 cut(s) 283
SchI GAGTC 1 cut(s) 1025
ScrFI CCNGG 2 cut(s) 360, 987
SduI GDGCHC 2 cut(s) 787, 1001
SfaNI GCATC 3 cut(s) 180, 958, 1183
SfcI CTRYAG 1 cut(s) 867
Sfr274I CTCGAG 1 cut(s) 545
Sfr303I CCGCGG 1 cut(s) 1231
SgrBI CCGCGG 1 cut(s) 1231
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
SpeI ACTAGT 1 cut(s) 500
Sse9I AATT 5 cut(s) 14, 93, 157, 457, 531
SsiI CCGC 5 cut(s) 589, 935, 964, 1228, 1230
SspMI CTAG 4 cut(s) 501, 978, 1043, 1083
SstI GAGCTC 1 cut(s) 787
StyD4I CCNGG 2 cut(s) 358, 985
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 3 cut(s) 431, 598, 1038
TaiI ACGT 1 cut(s) 739
TaqI TCGA 3 cut(s) 412, 546, 1162
TasI AATT 5 cut(s) 14, 93, 157, 457, 531
TatI WGTACW 1 cut(s) 1016
TauI GCSGC 1 cut(s) 937
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 4 cut(s) 155, 704, 1071, 1185
Tru9I TTAA 4 cut(s) 155, 704, 1071, 1185
TscAI CASTG 3 cut(s) 445, 603, 850
TseFI GTSAC 2 cut(s) 146, 169
TseI GCWGC 6 cut(s) 98, 293, 671, 971, 974, 1102
Tsp45I GTSAC 2 cut(s) 146, 169
TspDTI ATGAA 7 cut(s) 141, 197, 326, 440, 746, 758, 905
TspRI CASTG 3 cut(s) 445, 603, 850
Tth111I GACNNNGTC 1 cut(s) 648
Van91I CCANNNNNTGG 1 cut(s) 359
XapI RAATTY 1 cut(s) 157
XceI RCATGY 1 cut(s) 574
XhoI CTCGAG 1 cut(s) 545
XspI CTAG 4 cut(s) 501, 978, 1043, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.