Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008294

Physical Location & Seq

Genomic Coordinates Reverse (-)

4292700 .. 4299082

Gene Structure 6383 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1039500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1902 bp

ATGGGGGTTCCGCAGGCAATGGAGGCCCTCGAAGCGAGAGCTTCGTCTATAAGAGACGCGCTGCACAAGAGCCAGACCATCACCGACAACATGGTCGCCATTCTCGGCTCCTTCGACCACCGCCTCTCCGCCCTCGAAACCGCCATGCGTCCCACTCAGATTAGGACTCATTCGATTCGGAGGGCGCATGAGAACATTGATAAGACATTGAAGGCTGTAGAGGTTATATTGGGGCAATTCGACCTCACACGAAAGGCAGAGGCTAAAATACTGAGAGGGCCACATGAGGATTTGGAAAGCTATTTGGAAGCAATTGATCAGCTGAGAAGCATCATTCATTTCTTTAGTAGCCATAAAAATGTTAAGAGCAGTGATGGGGTGCTTCACCATGCCAATAACTTGCTTTCGAAAGCCATCTCGAAGCTCGAAGATGAGTTTAGACAGCTCCTCACAAATTACAGCAAGCCTGTGGAGCCTGATCGTCTATTTGATTGTCTCCCTGACTCTCTACGACCAGATCCAGCTGGACAGAAAAATGATGCTGGTGGAAAGGGTTCTGAACACCCAAACAAAAGTTTAAAACCTGTCATTTACACACCACTAACGCTTATTCCCCCAAGGGTTCTGCCATTACTGCATGATTTAGCCCAACAAATGGTTCTAGCTGGCCATCAACAACAGCTATTTAGGACCTACAGGGACACTCGTGCTTCTGTTTTGGAGCAGAGCTTGAGAAAACTAGGCGTGGAGAGACTTACTAAAGATGATGTCCAGAAAATGCAGTGGGAGGTTTTAGAGGCTAAGATTGGGAATTGGATACATTACATGCGAATTGCTGTGAAGCTGCTGTTTTCCGGGGAAAAGAAAATCTGTGATCAAATATTTGAAGGTGCTGAGACACTTAAGGATCCATGTTTTGCTGAAGTTACTGCAAACAGTGTGGCTATGCTCCTCAGTTTTGGGGAGGCTATTGCCAGAAGCAAGAGGTCACCTGAAAAGTTATTTGTTCTTTTAGACATGTATGAGATAATGAGGGACCTTCAGTCAGAGATTGAGTTACTTTTTGGAAGTAAAGCTTGCATGGAAATGCGGGAATCTGCATTAAGTTTGACAAAGCGTCTAGCTCAAACAGCCCAGGAAACTTTTGGTGATTTTGAAGAAGCTGTTGAAAAGGATGCCACGAAAACTGCTGTTCTTGATGGAACCGTCCATCCTTTGACAAGCTACGTGATAAACTATGTAAAGTTTCTCTTCGATTATCAGTCTACACTGAAGCAACTTTTTCAAGAGTTTGATGAAGGTGAGCCAGAGTCTCAGTTATCAAAGGTTACTACAAGGATTATGCAGGCTCTTCAGAACAACCTGGATGGAAAATCTAAGCAGTATAAAGATCCTGCACTCACTCAGTTATTTCTCATGAACAACATTCACTATATAGTGAGATCCGTGCGGAGGTCAGAAGCAAAGGATTTGTTGGGGGATGACTGGGTGCAGATACACCGAAGGATCGTGCAGCAGCATGCAAATCAGTATAAGAGGGTTTCTTGGGCAAAGATTCTGCAGTGTCTTACCGTTCAGGGTGGAAATCCATCAGGTGGTGATAGCAGTTCACTTTCAAGAGCAATGGTGAAAGATCGGTTCAAGACCTTCAACACGCAGTTTGAGGAGCTTCATCAAAGGCAATCTCAGTGGACAGTTCCTGACAGCGAATTGCGAGAGTCTTTAAGGCTAGCTGTTGCTGAAATCCTCTTGCCTGCTTACAGATCATTCATCAAACGTTTCGGGCCTATGGTCGAGAACGGAAAAAACCCGGGGAAGTATATAAAGCTACGTCCGGAGAATATTGAATCAATGCTGAATGAATTTTTTGAGAGTAAGACGTGGGGAGAACAGAAGCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0005815 GO:0005829 GO:0005856 GO:0005886 GO:0008150 GO:0009894 GO:0010506 GO:0015630 GO:0016020 GO:0016241 GO:0019222 GO:0031323 GO:0031329 GO:0034451 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043900 GO:0043903 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:0071944 GO:2000535

KEGG Pathways

Metabolic & Signaling

ko04910 map04910

Pfam Domains

Protein Families

Exo70

Protein Analysis

634

Amino Acids

72.27

Weight (kDa)

8.33

Isoelectric Point (pI)

48.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exo70_N	PF20669	69 - 151	6.9e-22	Exocyst complex component Exo70 N-terminal
Exo70_C	PF03081	271 - 621	2.8e-130	Exo70 exocyst complex subunit C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G52340
fragaria_vesca	FvH4_6g20230 FvH4_6g20230 FvH4_6g20230 FvH4_6g20230 FvH4_6g20230 FvH4_6g20230
malus_domestica	MD12G1039500.v1.1 MD14G1038200.v1.1
prunus_persica	Prupe.7G096000_v2.0.a1
pyrus_communis	pycom12g03360 pycom14g03300
rosa_chinensis	RchiOBHm_Chr3g0475801
rosa_laevigata	RLG00000023812 RLG00000023816
rosa_multiflora	Rmu_sc0000539.1_g000084
rosa_roxburghii	Rroxscaffold_6G00405870
rosa_rugosa	Rorug03G0151700
rosa_samantha	Rh3AG201300 Rh3BG231600 Rh3CG226400 Rh3DG226900
rosa_wichuraiana	Rw3G018310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	92
AccB7I	CCANNNNNTGG	2 cut(s)	655, 1595
AccI	GTMKAC	1 cut(s)	1265
AccII	CGCG	1 cut(s)	59
AccIII	TCCGGA	1 cut(s)	1834
AciI	CCGC	6 cut(s)	11, 121, 129, 141, 1090, 1450
AclI	AACGTT	1 cut(s)	1777
AclWI	GGATC	6 cut(s)	512, 902, 915, 1385, 1437, 1514
AcoI	YGGCCR	1 cut(s)	667
AcsI	RAATTY	1 cut(s)	1862
AcuI	CTGAAG	4 cut(s)	942, 1025, 1292, 1337
AfiI	CCNNNNNNNGG	3 cut(s)	655, 1452, 1595
AflII	CTTAAG	1 cut(s)	902
AflIII	ACRYGT	1 cut(s)	1017
AgsI	TTSAA	9 cut(s)	211, 887, 1157, 1169, 1286, 1617, 1642, 1651, 1847
AhdI	GACNNNNNGTC	2 cut(s)	1042, 1116
AjiI	CACGTC	1 cut(s)	1881
AjnI	CCWGG	2 cut(s)	1134, 1362
AjuI	GAANNNNNNNTTGG	2 cut(s)	642, 674
AloI	GAACNNNNNNTCC	2 cut(s)	1195, 1227
Alw26I	GTCTC	5 cut(s)	48, 500, 745, 890, 1317
AlwI	GGATC	6 cut(s)	512, 902, 915, 1385, 1437, 1514
AlwNI	CAGNNNCTG	1 cut(s)	1700
Ama87I	CYCGRG	1 cut(s)	1810
Aor13HI	TCCGGA	1 cut(s)	1834
AoxI	GGCC	4 cut(s)	24, 278, 667, 1784
ApeKI	GCWGC	4 cut(s)	61, 844, 1513, 1516
ApoI	RAATTY	1 cut(s)	1862
Asp700I	GAANNNNTTC	1 cut(s)	553
AspLEI	GCGC	2 cut(s)	61, 187
AspS9I	GGNCC	5 cut(s)	25, 278, 690, 1036, 1784
AsuC2I	CCSGG	3 cut(s)	856, 1811, 1812
AsuHPI	GGTGA	7 cut(s)	73, 377, 981, 1160, 1313, 1610, 1639
AsuII	TTCGAA	1 cut(s)	407
AsuNHI	GCTAGC	1 cut(s)	1729
AvaI	CYCGRG	1 cut(s)	1810
AvaII	GGWCC	2 cut(s)	690, 1036
BalI	TGGCCA	1 cut(s)	669
BamHI	GGATCC	1 cut(s)	907
BauI	CACGAG	1 cut(s)	705
BbvI	GCAGC	4 cut(s)	48, 831, 1525, 1528
BccI	CCATC	8 cut(s)	86, 368, 422, 678, 1193, 1218, 1361, 1597
BciT130I	CCWGG	2 cut(s)	1136, 1364
BciVI	GTATCC	1 cut(s)	810
BclI	TGATCA	2 cut(s)	316, 874
BcnI	CCSGG	3 cut(s)	856, 1811, 1812
BcoDI	GTCTC	5 cut(s)	48, 500, 745, 890, 1317
BfaI	CTAG	4 cut(s)	662, 740, 1121, 1730
BfmI	CTRYAG	3 cut(s)	216, 694, 1559
BfrI	CTTAAG	1 cut(s)	902
BfuI	GTATCC	1 cut(s)	810
BisI	GCNGC	4 cut(s)	62, 845, 1514, 1517
BlsI	GCNGC	4 cut(s)	63, 846, 1515, 1518
Bme1390I	CCNGG	5 cut(s)	856, 1136, 1364, 1811, 1812
Bme18I	GGWCC	2 cut(s)	690, 1036
BmeRI	GACNNNNNGTC	2 cut(s)	1042, 1116
BmeT110I	CYCGRG	1 cut(s)	1810
BmgBI	CACGTC	1 cut(s)	1881
BmgT120I	GGNCC	5 cut(s)	25, 278, 690, 1036, 1784
BmiI	GGNNCC	6 cut(s)	9, 109, 474, 909, 1037, 1204
BmrFI	CCNGG	5 cut(s)	856, 1136, 1364, 1811, 1812
BmrI	ACTGGG	1 cut(s)	1495
BmsI	GCATC	3 cut(s)	339, 529, 1165
BmtI	GCTAGC	1 cut(s)	1733
BmuI	ACTGGG	1 cut(s)	1495
Bpu14I	TTCGAA	1 cut(s)	407
BpuEI	CTTGAG	1 cut(s)	751
BpuMI	CCSGG	3 cut(s)	856, 1811, 1812
BsaAI	YACGTR	1 cut(s)	1228
BsaJI	CCNNGG	5 cut(s)	617, 855, 1134, 1810, 1811
BsaWI	WCCGGW	1 cut(s)	1834
BsaXI	ACNNNNNCTCC	2 cut(s)	110, 140
Bsc4I	CCNNNNNNNGG	3 cut(s)	655, 1452, 1595
Bse1I	ACTGG	1 cut(s)	1490
Bse3DI	GCAATG	2 cut(s)	24, 1629
BseAI	TCCGGA	1 cut(s)	1834
BseBI	CCWGG	2 cut(s)	1136, 1364
BseDI	CCNNGG	5 cut(s)	617, 855, 1134, 1810, 1811
BseGI	GGATG	4 cut(s)	1180, 1210, 1372, 1486
BseLI	CCNNNNNNNGG	3 cut(s)	655, 1452, 1595
BseMI	GCAATG	2 cut(s)	24, 1629
BseMII	CTCAG	8 cut(s)	170, 263, 314, 885, 967, 1328, 1418, 1700
BseNI	ACTGG	1 cut(s)	1490
BseRI	GAGGAG	3 cut(s)	437, 941, 1679
BseXI	GCAGC	4 cut(s)	48, 831, 1525, 1528
BsgI	GTGCAG	4 cut(s)	47, 1380, 1511, 1532
Bsh1236I	CGCG	1 cut(s)	59
BshFI	GGCC	4 cut(s)	26, 280, 669, 1786
BsiHKCI	CYCGRG	1 cut(s)	1810
BsiSI	CCGG	3 cut(s)	855, 1811, 1835
BslFI	GGGAC	3 cut(s)	135, 713, 1049
BslI	CCNNNNNNNGG	3 cut(s)	655, 1452, 1595
BsmAI	GTCTC	5 cut(s)	48, 500, 745, 890, 1317
BsmBI	CGTCTC	1 cut(s)	48
BsmFI	GGGAC	3 cut(s)	135, 713, 1049
BsnI	GGCC	4 cut(s)	26, 280, 669, 1786
BsoBI	CYCGRG	1 cut(s)	1810
Bsp119I	TTCGAA	1 cut(s)	407
Bsp13I	TCCGGA	1 cut(s)	1834
BspACI	CCGC	6 cut(s)	11, 121, 129, 141, 1090, 1450
BspANI	GGCC	4 cut(s)	26, 280, 669, 1786
BspCNI	CTCAG	8 cut(s)	169, 264, 315, 886, 966, 1327, 1417, 1699
BspEI	TCCGGA	1 cut(s)	1834
BspFNI	CGCG	1 cut(s)	59
BspHI	TCATGA	1 cut(s)	1416
BspLI	GGNNCC	6 cut(s)	9, 109, 474, 909, 1037, 1204
BspMAI	CTGCAG	1 cut(s)	1563
BspOI	GCTAGC	1 cut(s)	1733
BspPI	GGATC	6 cut(s)	512, 902, 915, 1385, 1437, 1514
BspQI	GCTCTTC	1 cut(s)	1356
BspT104I	TTCGAA	1 cut(s)	407
BspTI	CTTAAG	1 cut(s)	902
BsrDI	GCAATG	2 cut(s)	24, 1629
BsrI	ACTGG	1 cut(s)	1490
BssECI	CCNNGG	5 cut(s)	617, 855, 1134, 1810, 1811
BssSI	CACGAG	1 cut(s)	705
BssT1I	CCWWGG	1 cut(s)	617
Bst2BI	CACGAG	1 cut(s)	705
Bst2UI	CCWGG	2 cut(s)	1136, 1364
Bst4CI	ACNGT	4 cut(s)	938, 1207, 1573, 1696
Bst6I	CTCTTC	2 cut(s)	1256, 1356
BstAFI	CTTAAG	1 cut(s)	902
BstBAI	YACGTR	1 cut(s)	1228
BstBI	TTCGAA	1 cut(s)	407
BstC8I	GCNNGC	8 cut(s)	15, 464, 667, 1078, 1347, 1521, 1731, 1755
BstEII	GGTNACC	1 cut(s)	987
BstF5I	GGATG	4 cut(s)	1180, 1210, 1372, 1486
BstFNI	CGCG	1 cut(s)	59
BstHHI	GCGC	2 cut(s)	61, 187
BstMAI	GTCTC	5 cut(s)	48, 500, 745, 890, 1317
BstMWI	GCNNNNNNNGC	5 cut(s)	23, 32, 472, 634, 1130
BstNI	CCWGG	2 cut(s)	1136, 1364
BstNSI	RCATGY	3 cut(s)	829, 1021, 1523
BstPI	GGTNACC	1 cut(s)	987
BstSCI	CCNGG	5 cut(s)	854, 1134, 1362, 1809, 1810
BstSFI	CTRYAG	3 cut(s)	216, 694, 1559
BstUI	CGCG	1 cut(s)	59
BstV1I	GCAGC	4 cut(s)	48, 831, 1525, 1528
BstX2I	RGATCY	4 cut(s)	517, 907, 1390, 1442
BstYI	RGATCY	4 cut(s)	517, 907, 1390, 1442
BsuI	GTATCC	1 cut(s)	810
BsuRI	GGCC	4 cut(s)	26, 280, 669, 1786
BtrI	CACGTC	1 cut(s)	1881
BtsCI	GGATG	4 cut(s)	1180, 1210, 1372, 1486
BtsI	GCAGTG	3 cut(s)	376, 788, 1568
BtsIMutI	CAGTG	6 cut(s)	376, 788, 943, 1268, 1568, 1694
Cac8I	GCNNGC	8 cut(s)	15, 464, 667, 1078, 1347, 1521, 1731, 1755
CaiI	CAGNNNCTG	1 cut(s)	1700
CciI	TCATGA	1 cut(s)	1416
CfoI	GCGC	2 cut(s)	61, 187
Cfr13I	GGNCC	5 cut(s)	25, 278, 690, 1036, 1784
Cfr9I	CCCGGG	1 cut(s)	1810
CseI	GACGC	3 cut(s)	65, 137, 1106
CspCI	CAANNNNNGTGG	4 cut(s)	921, 956, 1670, 1705
DraI	TTTAAA	1 cut(s)	579
DrdI	GACNNNNNNGTC	1 cut(s)	92
DriI	GACNNNNNGTC	2 cut(s)	1042, 1116
DseDI	GACNNNNNNGTC	1 cut(s)	92
EaeI	YGGCCR	1 cut(s)	667
Eam1104I	CTCTTC	2 cut(s)	1256, 1356
Eam1105I	GACNNNNNGTC	2 cut(s)	1042, 1116
EarI	CTCTTC	2 cut(s)	1256, 1356
EciI	GGCGGA	1 cut(s)	118
Eco130I	CCWWGG	1 cut(s)	617
Eco47I	GGWCC	2 cut(s)	690, 1036
Eco57I	CTGAAG	4 cut(s)	942, 1025, 1292, 1337
Eco88I	CYCGRG	1 cut(s)	1810
Eco91I	GGTNACC	1 cut(s)	987
EcoO109I	RGGNCCY	3 cut(s)	25, 690, 1036
EcoO65I	GGTNACC	1 cut(s)	987
EcoRII	CCWGG	2 cut(s)	1134, 1362
EcoT14I	CCWWGG	1 cut(s)	617
ErhI	CCWWGG	1 cut(s)	617
Esp3I	CGTCTC	1 cut(s)	48
FalI	AAGNNNNNCTT	4 cut(s)	1060, 1092, 1235, 1267
FaqI	GGGAC	3 cut(s)	135, 713, 1049
FauI	CCCGC	1 cut(s)	1083
FbaI	TGATCA	2 cut(s)	316, 874
FblI	GTMKAC	1 cut(s)	1265
Fnu4HI	GCNGC	4 cut(s)	62, 845, 1514, 1517
FokI	GGATG	4 cut(s)	1187, 1197, 1379, 1493
Fsp4HI	GCNGC	4 cut(s)	62, 845, 1514, 1517
FspBI	CTAG	4 cut(s)	662, 740, 1121, 1730
GlaI	GCGC	2 cut(s)	60, 186
GluI	GCNGC	4 cut(s)	62, 845, 1514, 1517
HaeIII	GGCC	4 cut(s)	26, 280, 669, 1786
HapII	CCGG	3 cut(s)	855, 1811, 1835
HgaI	GACGC	3 cut(s)	65, 137, 1106
HhaI	GCGC	2 cut(s)	61, 187
Hin6I	GCGC	2 cut(s)	59, 185
HinP1I	GCGC	2 cut(s)	59, 185
HindIII	AAGCTT	1 cut(s)	1074
HinfI	GANTC	8 cut(s)	166, 175, 503, 1094, 1310, 1555, 1718, 1847
HpaII	CCGG	3 cut(s)	855, 1811, 1835
HphI	GGTGA	7 cut(s)	73, 377, 981, 1160, 1313, 1610, 1639
Hpy166II	GTNNAC	3 cut(s)	1266, 1610, 1692
Hpy188I	TCNGA	6 cut(s)	159, 180, 559, 1048, 1356, 1459
Hpy8I	GTNNAC	3 cut(s)	1266, 1610, 1692
HpyAV	CCTTC	7 cut(s)	121, 205, 881, 1049, 1292, 1497, 1657
HpyCH4III	ACNGT	4 cut(s)	938, 1207, 1573, 1696
HpyCH4IV	ACGT	4 cut(s)	1227, 1777, 1831, 1880
HpyF10VI	GCNNNNNNNGC	5 cut(s)	23, 32, 472, 634, 1130
HpySE526I	ACGT	4 cut(s)	1227, 1777, 1831, 1880
HspAI	GCGC	2 cut(s)	59, 185
Kpn2I	TCCGGA	1 cut(s)	1834
Ksp22I	TGATCA	2 cut(s)	316, 874
LguI	GCTCTTC	1 cut(s)	1356
LmnI	GCTCC	6 cut(s)	113, 450, 472, 721, 954, 1666
Lsp1109I	GCAGC	4 cut(s)	48, 831, 1525, 1528
LweI	GCATC	3 cut(s)	339, 529, 1165
MaeI	CTAG	4 cut(s)	662, 740, 1121, 1730
MaeII	ACGT	4 cut(s)	1227, 1777, 1831, 1880
MaeIII	GTNAC	4 cut(s)	925, 987, 1056, 1327
MboII	GAAGA	4 cut(s)	440, 1169, 1243, 1343
MfeI	CAATTG	1 cut(s)	312
MflI	RGATCY	4 cut(s)	517, 907, 1390, 1442
MlsI	TGGCCA	1 cut(s)	669
MluCI	AATT	7 cut(s)	236, 312, 454, 811, 831, 1709, 1862
MluNI	TGGCCA	1 cut(s)	669
MlyI	GAGTC	4 cut(s)	160, 497, 1319, 1727
Mox20I	TGGCCA	1 cut(s)	669
MroI	TCCGGA	1 cut(s)	1834
MroXI	GAANNNNTTC	1 cut(s)	553
MscI	TGGCCA	1 cut(s)	669
MseI	TTAA	5 cut(s)	363, 578, 903, 1103, 1724
MslI	CAYNNNNRTG	2 cut(s)	357, 1085
Msp20I	TGGCCA	1 cut(s)	669
MspA1I	CMGCKG	2 cut(s)	322, 524
MspCI	CTTAAG	1 cut(s)	902
MspI	CCGG	3 cut(s)	855, 1811, 1835
MspR9I	CCNGG	5 cut(s)	856, 1136, 1364, 1811, 1812
MunI	CAATTG	1 cut(s)	312
MvaI	CCWGG	2 cut(s)	1136, 1364
MvnI	CGCG	1 cut(s)	59
MwoI	GCNNNNNNNGC	5 cut(s)	23, 32, 472, 634, 1130
NciI	CCSGG	3 cut(s)	856, 1811, 1812
NheI	GCTAGC	1 cut(s)	1729
NlaIV	GGNNCC	6 cut(s)	9, 109, 474, 909, 1037, 1204
NmeAIII	GCCGAG	1 cut(s)	84
NmuCI	GTSAC	1 cut(s)	987
NspI	RCATGY	3 cut(s)	829, 1021, 1523
NspV	TTCGAA	1 cut(s)	407
PaeI	GCATGC	1 cut(s)	1523
PagI	TCATGA	1 cut(s)	1416
PciI	ACATGT	1 cut(s)	1017
PciSI	GCTCTTC	1 cut(s)	1356
PcsI	WCGNNNNNNNCGW	1 cut(s)	111
PdmI	GAANNNNTTC	1 cut(s)	553
PfeI	GAWTC	4 cut(s)	175, 1094, 1555, 1847
PflMI	CCANNNNNTGG	2 cut(s)	655, 1595
PkrI	GCNGC	4 cut(s)	63, 846, 1515, 1518
PleI	GAGTC	4 cut(s)	160, 497, 1318, 1726
PpsI	GAGTC	4 cut(s)	160, 497, 1318, 1726
Ppu21I	YACGTR	1 cut(s)	1228
PpuMI	RGGWCCY	2 cut(s)	690, 1036
PscI	ACATGT	1 cut(s)	1017
Psp1406I	AACGTT	1 cut(s)	1777
Psp5II	RGGWCCY	2 cut(s)	690, 1036
Psp6I	CCWGG	2 cut(s)	1134, 1362
PspEI	GGTNACC	1 cut(s)	987
PspGI	CCWGG	2 cut(s)	1134, 1362
PspN4I	GGNNCC	6 cut(s)	9, 109, 474, 909, 1037, 1204
PspPI	GGNCC	5 cut(s)	25, 278, 690, 1036, 1784
PspPPI	RGGWCCY	2 cut(s)	690, 1036
PstI	CTGCAG	1 cut(s)	1563
PstNI	CAGNNNCTG	1 cut(s)	1700
PsuI	RGATCY	4 cut(s)	517, 907, 1390, 1442
PvuII	CAGCTG	2 cut(s)	322, 524
RseI	CAYNNNNRTG	2 cut(s)	357, 1085
SapI	GCTCTTC	1 cut(s)	1356
SaqAI	TTAA	5 cut(s)	363, 578, 903, 1103, 1724
SatI	GCNGC	4 cut(s)	62, 845, 1514, 1517
Sau96I	GGNCC	5 cut(s)	25, 278, 690, 1036, 1784
SchI	GAGTC	4 cut(s)	160, 497, 1319, 1727
ScrFI	CCNGG	5 cut(s)	856, 1136, 1364, 1811, 1812
SfaNI	GCATC	3 cut(s)	339, 529, 1165
SfcI	CTRYAG	3 cut(s)	216, 694, 1559
SfuI	TTCGAA	1 cut(s)	407
SinI	GGWCC	2 cut(s)	690, 1036
SmaI	CCCGGG	1 cut(s)	1812
SmiMI	CAYNNNNRTG	2 cut(s)	357, 1085
SmlI	CTYRAG	2 cut(s)	730, 902
SmoI	CTYRAG	2 cut(s)	730, 902
SphI	GCATGC	1 cut(s)	1523
Sse9I	AATT	7 cut(s)	236, 312, 454, 811, 831, 1709, 1862
SsiI	CCGC	6 cut(s)	11, 121, 129, 141, 1090, 1450
SspI	AATATT	2 cut(s)	882, 1843
SspMI	CTAG	4 cut(s)	662, 740, 1121, 1730
StyD4I	CCNGG	5 cut(s)	854, 1134, 1362, 1809, 1810
StyI	CCWWGG	1 cut(s)	617
TaaI	ACNGT	4 cut(s)	938, 1207, 1573, 1696
TaiI	ACGT	4 cut(s)	1230, 1780, 1834, 1883
TasI	AATT	7 cut(s)	236, 312, 454, 811, 831, 1709, 1862
TfiI	GAWTC	4 cut(s)	175, 1094, 1555, 1847
Tru1I	TTAA	5 cut(s)	363, 578, 903, 1103, 1724
Tru9I	TTAA	5 cut(s)	363, 578, 903, 1103, 1724
TscAI	CASTG	6 cut(s)	376, 788, 943, 1275, 1568, 1694
TseFI	GTSAC	1 cut(s)	987
TseI	GCWGC	4 cut(s)	61, 844, 1513, 1516
Tsp45I	GTSAC	1 cut(s)	987
TspDTI	ATGAA	6 cut(s)	326, 1311, 1433, 1661, 1759, 1875
TspGWI	ACGGA	2 cut(s)	1435, 1815
TspMI	CCCGGG	1 cut(s)	1810
TspRI	CASTG	6 cut(s)	376, 788, 943, 1275, 1568, 1694
Van91I	CCANNNNNTGG	2 cut(s)	655, 1595
Vha464I	CTTAAG	1 cut(s)	902
VpaK11BI	GGWCC	2 cut(s)	690, 1036
XapI	RAATTY	1 cut(s)	1862
XceI	RCATGY	3 cut(s)	829, 1021, 1523
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1142
XmaI	CCCGGG	1 cut(s)	1810
XmiI	GTMKAC	1 cut(s)	1265
XmnI	GAANNNNTTC	1 cut(s)	553
XspI	CTAG	4 cut(s)	662, 740, 1121, 1730

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.