MD12G1063100.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016410
Physical Location & Seq
Reverse (-)
7382927 .. 7389510
6584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1063100.v1.1.491

Sequence Viewer

Length: 1551 bp
ATGAAGCTGCAATCTTTGAACAATGGCTATGCAATCCGAGCCCTCAGCCCCAAACCCAATTGCTCCGCAGAGCTGAAACTCCCAGCAACCGTCCGATCATGCGCTCCGGAACAGGACCTTCGAGGCCAATCCGTTGCTATTGTTGGGTTGGGAAAATCTGGGAGAGCTGCGGCCAGGCTTGCTCTTGCCAGAGGTGCTTCAGTTTTCGCCTTCGATCAGAACCCGAATCTGCGTCTTTTAGAGAAAGATCCGCTCTTCAAGAAGCAATATAACGGAATAGGTGGATTGAAAACAATCCTGGGTGAATTTGACGAGGAGCTACTTAATAATGCGGACAGGGTTGTGGTGTCCCCTGGAGTTCCCCTGCAAAACTATGGTCTTTCCTCTTTGTTGCAGTCGGGAAAGCAGGTAATGTCTGAGTTGGACTTTGCTGCTGAAATTCTCCCAAAAAGTGTAAAGATTTTAGCAGTGACAGGGACCAATGGAAAATCAACTGTAGTCACTTTTGCTGGACAGATGCTTAGTCATTTTGGAATCGAGACATTTGTTGGGGGTAACCTTGGTAACCCACTCTCAGAGGCTGCTTTCCAATGCTTACAGCCACCTTCTTCAAAACCCAAGTTTAAGGTTGCAGTTGTGGAGGTAAGCAGTTATCAAATGGAGATTCCCAGCAAGTACTTCTGCCCTTCTGTTGCTGTGGTGTTAAACCTTACACCTGATCATCTAGAAAGGCACGGAACAATGAGGGATTATGCACTGACTAAATGCCGGTTATTTTTTCACATGACTGGTGCCAAGCTTGGGCTTCTTTGTTTTGGAAACCAGTACTTAGATGAAGCAATTATTGAAAGCTTGAATGAACTTAATCTTGCGTGGATAGGAGCCATTCCAGGTGTGAAAATTGACATGGAGACAAAAATTGCAAGCTTTGAAGTGCCTGCATTGGGAGTCAATTCACAACTACAATTGGGGGCAATGAAAACGATGGGGGGACACAACTTTCACAATGCTGCGGTGGCTGTTCTGTCTGTTGTTGGACTGAATGTTGGAGTTGATGTTGAAGCGATAGGTCCATGTATTGAAAAATTAAGGGCACCACCTCATCGTATGCAAATTGTATGCAAGGATATCCATGGAGTAACCTGGGTCGATGACAGTAAGGCAACAAATGTGGAAGCAACATACGCAGGACTAATGGGTCTAAAGAAACAAAAGTCTGTGGTTCTACTTGGGGGCCTTGCGAAGGTGTTAGATGGACAAGAGTGTAATGGTTTTGAACGATTGATAGAACCTTTGAAGTACCAGAGATGTGTAATCACGTTTGGGTCTTCCGGAATGCTGATTAAGAAGACGCTGTCTGATAACGGTCTTAGCATTCCCTGCATCAGAGCCGTAAATCTTAAGGAAGCTGTCAACTGGGCTAGGAGTATGGCAGAACATGGGGACAGTATTGTATTAAGTCCAGGTTGTGCAAGCTTTGATGAATTTAGAAATTTTGAGCACAGAGGAATGTTTTTCCAGGACCTAGCATTCACATACGTATCTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2-Hacid_dh_C Mur_ligase_C Mur_ligase_M

Protein Analysis

517

Amino Acids

55.71

Weight (kDa)

8.35

Isoelectric Point (pI)

31.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MurD-like_N PF21799 44 - 138 1.6e-11 Mur ligase MurD-like, N-terminal domain
Mur_ligase_M PF08245 157 - 342 5.1e-20 Mur ligase middle domain
Mur_ligase_C PF02875 368 - 488 3.6e-08 Mur ligase, glutamate ligase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016410)

Species Orthologous Gene IDs
malus_domestica MD12G1063100.v1.1
prunus_persica Prupe.7G070400_v2.0.a1
pyrus_communis pycom12g05560
rosa_chinensis RchiOBHm_Chr3g0480711 RchiOBHm_Chr3g0480721
rosa_multiflora Rmu_sc0001135.1_g000007 Rmu_sc0003902.1_g000026
rosa_roxburghii Rroxscaffold_6G00401140
rosa_rugosa Rorug03G0185000 Rorug03G0185100
rosa_samantha Rh3AG237200 Rh3BG270300 Rh3CG266500 Rh3DG262700
rosa_wichuraiana Rw3G021240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1197
Acc36I ACCTGC 1 cut(s) 397
AccB1I GGYRCC 2 cut(s) 791, 1093
AccBSI CCGCTC 1 cut(s) 253
AccIII TCCGGA 2 cut(s) 106, 1331
AciI CCGC 5 cut(s) 66, 170, 251, 332, 1013
AclWI GGATC 1 cut(s) 242
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 4 cut(s) 305, 438, 1484, 1492
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 3 cut(s) 677, 827, 1301
AfiI CCNNNNNNNGG 3 cut(s) 801, 944, 1243
AflII CTTAAG 1 cut(s) 1400
AjnI CCWGG 7 cut(s) 173, 297, 352, 889, 1142, 1462, 1518
AluBI AGCT 9 cut(s) 7, 73, 167, 319, 799, 852, 927, 1409, 1476
AluI AGCT 9 cut(s) 7, 73, 167, 319, 799, 852, 927, 1409, 1476
Alw21I GWGCWC 1 cut(s) 1503
Alw26I GTCTC 2 cut(s) 533, 905
AlwI GGATC 1 cut(s) 242
AlwNI CAGNNNCTG 1 cut(s) 581
Aor13HI TCCGGA 2 cut(s) 106, 1331
AoxI GGCC 3 cut(s) 124, 171, 1234
ApeKI GCWGC 5 cut(s) 7, 167, 431, 581, 1010
ApoI RAATTY 4 cut(s) 305, 438, 1484, 1492
ArsI GACNNNNNNTTYG 2 cut(s) 361, 393
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 5 cut(s) 115, 477, 1070, 1234, 1522
AsuHPI GGTGA 1 cut(s) 314
AvaII GGWCC 4 cut(s) 115, 477, 1070, 1522
BaeGI GKGCMC 1 cut(s) 1096
BaeI ACNNNNGTAYC 1 cut(s) 1524
BanI GGYRCC 2 cut(s) 791, 1093
BanII GRGCYC 1 cut(s) 43
BbsI GAAGAC 2 cut(s) 1320, 1355
Bbv12I GWGCWC 1 cut(s) 1503
BbvCI CCTCAGC 1 cut(s) 44
BbvI GCAGC 4 cut(s) 154, 418, 568, 997
BccI CCATC 2 cut(s) 979, 1247
BceAI ACGGC 1 cut(s) 1376
BciT130I CCWGG 7 cut(s) 175, 299, 354, 891, 1144, 1464, 1520
BclI TGATCA 1 cut(s) 718
BcoDI GTCTC 2 cut(s) 533, 905
BfaI CTAG 3 cut(s) 725, 1422, 1526
BfmI CTRYAG 1 cut(s) 495
BfrI CTTAAG 1 cut(s) 1400
BfuAI ACCTGC 1 cut(s) 397
BisI GCNGC 6 cut(s) 8, 168, 171, 432, 582, 1011
BlsI GCNGC 6 cut(s) 9, 169, 172, 433, 583, 1012
BmcAI AGTACT 2 cut(s) 677, 827
Bme1390I CCNGG 7 cut(s) 175, 299, 354, 891, 1144, 1464, 1520
Bme18I GGWCC 4 cut(s) 115, 477, 1070, 1522
BmgT120I GGNCC 5 cut(s) 115, 477, 1070, 1234, 1522
BmiI GGNNCC 5 cut(s) 478, 793, 883, 1095, 1235
BmrFI CCNGG 7 cut(s) 175, 299, 354, 891, 1144, 1464, 1520
BmrI ACTGGG 1 cut(s) 1426
BmsI GCATC 2 cut(s) 507, 1392
BmuI ACTGGG 1 cut(s) 1426
BpiI GAAGAC 2 cut(s) 1320, 1355
BpmI CTGGAG 1 cut(s) 375
Bpu10I CCTNAGC 1 cut(s) 44
BsaAI YACGTR 1 cut(s) 1540
BsaJI CCNNGG 5 cut(s) 298, 352, 559, 1132, 1143
BsaWI WCCGGW 2 cut(s) 106, 1331
BsaXI ACNNNNNCTCC 2 cut(s) 1041, 1071
Bsc4I CCNNNNNNNGG 3 cut(s) 801, 944, 1243
Bse118I RCCGGY 1 cut(s) 768
Bse1I ACTGG 3 cut(s) 793, 823, 1421
Bse3DI GCAATG 1 cut(s) 981
BseAI TCCGGA 2 cut(s) 106, 1331
BseBI CCWGG 7 cut(s) 175, 299, 354, 891, 1144, 1464, 1520
BseDI CCNNGG 5 cut(s) 298, 352, 559, 1132, 1143
BseLI CCNNNNNNNGG 3 cut(s) 801, 944, 1243
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 3 cut(s) 58, 408, 588
BseNI ACTGG 3 cut(s) 793, 823, 1421
BseRI GAGGAG 1 cut(s) 329
BseSI GKGCMC 1 cut(s) 1096
BseXI GCAGC 4 cut(s) 154, 418, 568, 997
BseYI CCCAGC 2 cut(s) 82, 668
BshFI GGCC 3 cut(s) 126, 173, 1236
BshNI GGYRCC 2 cut(s) 791, 1093
BsiHKAI GWGCWC 1 cut(s) 1503
BsiSI CCGG 3 cut(s) 107, 769, 1332
BslFI GGGAC 4 cut(s) 334, 490, 1005, 1457
BslI CCNNNNNNNGG 3 cut(s) 801, 944, 1243
BsmAI GTCTC 2 cut(s) 533, 905
BsmFI GGGAC 4 cut(s) 334, 490, 1005, 1457
BsmI GAATGC 3 cut(s) 1341, 1374, 1529
BsnI GGCC 3 cut(s) 126, 173, 1236
Bsp1286I GDGCHC 3 cut(s) 43, 1096, 1503
Bsp13I TCCGGA 2 cut(s) 106, 1331
Bsp143I GATC 4 cut(s) 95, 214, 247, 718
Bsp19I CCATGG 1 cut(s) 1132
BspACI CCGC 5 cut(s) 66, 170, 251, 332, 1013
BspANI GGCC 3 cut(s) 126, 173, 1236
BspCNI CTCAG 3 cut(s) 57, 409, 587
BspEI TCCGGA 2 cut(s) 106, 1331
BspLI GGNNCC 5 cut(s) 478, 793, 883, 1095, 1235
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 1 cut(s) 242
BspQI GCTCTTC 1 cut(s) 260
BspT107I GGYRCC 2 cut(s) 791, 1093
BspTI CTTAAG 1 cut(s) 1400
BsrBI CCGCTC 1 cut(s) 253
BsrDI GCAATG 1 cut(s) 981
BsrFI RCCGGY 1 cut(s) 768
BsrI ACTGG 3 cut(s) 793, 823, 1421
BssAI RCCGGY 1 cut(s) 768
BssECI CCNNGG 5 cut(s) 298, 352, 559, 1132, 1143
BssMI GATC 4 cut(s) 95, 214, 247, 718
BssT1I CCWWGG 2 cut(s) 559, 1132
Bst2UI CCWGG 7 cut(s) 175, 299, 354, 891, 1144, 1464, 1520
Bst4CI ACNGT 5 cut(s) 91, 496, 1157, 1367, 1448
Bst6I CTCTTC 1 cut(s) 260
BstAFI CTTAAG 1 cut(s) 1400
BstAPI GCANNNNNTGC 1 cut(s) 1380
BstBAI YACGTR 1 cut(s) 1540
BstC8I GCNNGC 4 cut(s) 180, 925, 939, 1474
BstDEI CTNAG 6 cut(s) 44, 417, 521, 574, 829, 1370
BstDSI CCRYGG 1 cut(s) 1132
BstEII GGTNACC 2 cut(s) 554, 563
BstENI CCTNNNNNAGG 1 cut(s) 1241
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 4 cut(s) 98, 217, 250, 721
BstMAI GTCTC 2 cut(s) 533, 905
BstMBI GATC 4 cut(s) 95, 214, 247, 718
BstMWI GCNNNNNNNGC 6 cut(s) 38, 179, 194, 1016, 1184, 1380
BstNI CCWGG 7 cut(s) 175, 299, 354, 891, 1144, 1464, 1520
BstPI GGTNACC 2 cut(s) 554, 563
BstSCI CCNGG 7 cut(s) 173, 297, 352, 889, 1142, 1462, 1518
BstSFI CTRYAG 1 cut(s) 495
BstSLI GKGCMC 1 cut(s) 1096
BstSNI TACGTA 1 cut(s) 1540
BstV1I GCAGC 4 cut(s) 154, 418, 568, 997
BstV2I GAAGAC 2 cut(s) 1320, 1355
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuRI GGCC 3 cut(s) 126, 173, 1236
BtgI CCRYGG 1 cut(s) 1132
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 2 cut(s) 474, 755
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 4 cut(s) 180, 925, 939, 1474
CaiI CAGNNNCTG 1 cut(s) 581
CfoI GCGC 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 768
Cfr13I GGNCC 5 cut(s) 115, 477, 1070, 1234, 1522
CseI GACGC 2 cut(s) 221, 1360
Csp6I GTAC 3 cut(s) 676, 826, 1300
CspCI CAANNNNNGTGG 2 cut(s) 1152, 1187
CviAII CATG 6 cut(s) 99, 784, 907, 1074, 1133, 1439
CviQI GTAC 3 cut(s) 676, 826, 1300
DdeI CTNAG 6 cut(s) 44, 417, 521, 574, 829, 1370
DpnI GATC 4 cut(s) 97, 216, 249, 720
DpnII GATC 4 cut(s) 95, 214, 247, 718
DrdI GACNNNNNNGTC 1 cut(s) 1197
DseDI GACNNNNNNGTC 1 cut(s) 1197
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco105I TACGTA 1 cut(s) 1540
Eco130I CCWWGG 2 cut(s) 559, 1132
Eco24I GRGCYC 1 cut(s) 43
Eco32I GATATC 1 cut(s) 1129
Eco47I GGWCC 4 cut(s) 115, 477, 1070, 1522
Eco57I CTGAAG 1 cut(s) 183
Eco91I GGTNACC 2 cut(s) 554, 563
EcoNI CCTNNNNNAGG 1 cut(s) 1241
EcoO109I RGGNCCY 3 cut(s) 115, 1234, 1522
EcoO65I GGTNACC 2 cut(s) 554, 563
EcoRII CCWGG 7 cut(s) 173, 297, 352, 889, 1142, 1462, 1518
EcoRV GATATC 1 cut(s) 1129
EcoT14I CCWWGG 2 cut(s) 559, 1132
EcoT38I GRGCYC 1 cut(s) 43
ErhI CCWWGG 2 cut(s) 559, 1132
FaeI CATG 6 cut(s) 102, 787, 910, 1077, 1136, 1442
FaqI GGGAC 4 cut(s) 334, 490, 1005, 1457
FatI CATG 6 cut(s) 98, 783, 906, 1073, 1132, 1438
FbaI TGATCA 1 cut(s) 718
Fnu4HI GCNGC 6 cut(s) 8, 168, 171, 432, 582, 1011
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 6 cut(s) 8, 168, 171, 432, 582, 1011
FspBI CTAG 3 cut(s) 725, 1422, 1526
GlaI GCGC 1 cut(s) 103
GluI GCNGC 6 cut(s) 8, 168, 171, 432, 582, 1011
GsaI CCCAGC 2 cut(s) 86, 672
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 3 cut(s) 126, 173, 1236
HapII CCGG 3 cut(s) 107, 769, 1332
HgaI GACGC 2 cut(s) 221, 1360
HhaI GCGC 1 cut(s) 104
Hin1II CATG 6 cut(s) 102, 787, 910, 1077, 1136, 1442
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 1414
HindII GTYRAC 1 cut(s) 1414
HindIII AAGCTT 4 cut(s) 797, 850, 925, 1474
HinfI GANTC 4 cut(s) 226, 534, 664, 948
HpaII CCGG 3 cut(s) 107, 769, 1332
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 1 cut(s) 1414
Hpy188I TCNGA 7 cut(s) 38, 95, 219, 418, 577, 1360, 1388
Hpy188III TCNNGA 6 cut(s) 107, 259, 399, 538, 725, 1332
Hpy8I GTNNAC 1 cut(s) 1414
HpyAV CCTTC 5 cut(s) 128, 220, 615, 696, 1237
HpyCH4III ACNGT 5 cut(s) 91, 496, 1157, 1367, 1448
HpyCH4IV ACGT 2 cut(s) 1319, 1539
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 179, 194, 1016, 1184, 1380
HpyF3I CTNAG 6 cut(s) 44, 417, 521, 574, 829, 1370
HpySE526I ACGT 2 cut(s) 1319, 1539
Hsp92II CATG 6 cut(s) 102, 787, 910, 1077, 1136, 1442
HspAI GCGC 1 cut(s) 102
Kpn2I TCCGGA 2 cut(s) 106, 1331
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 4 cut(s) 95, 214, 247, 718
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 4 cut(s) 68, 109, 316, 881
Lsp1109I GCAGC 4 cut(s) 154, 418, 568, 997
LweI GCATC 2 cut(s) 507, 1392
MaeI CTAG 3 cut(s) 725, 1422, 1526
MaeII ACGT 2 cut(s) 1319, 1539
MaeIII GTNAC 5 cut(s) 469, 499, 554, 563, 1138
MalI GATC 4 cut(s) 97, 216, 249, 720
MbiI CCGCTC 1 cut(s) 253
MboI GATC 4 cut(s) 95, 214, 247, 718
MboII GAAGA 4 cut(s) 247, 600, 1320, 1360
MfeI CAATTG 2 cut(s) 58, 965
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 3 cut(s) 43, 1096, 1503
MlyI GAGTC 1 cut(s) 957
MmeI TCCRAC 3 cut(s) 402, 1015, 1027
MroI TCCGGA 2 cut(s) 106, 1331
MseI TTAA 8 cut(s) 324, 624, 704, 864, 1088, 1344, 1401, 1457
MspCI CTTAAG 1 cut(s) 1400
MspI CCGG 3 cut(s) 107, 769, 1332
MspR9I CCNGG 7 cut(s) 175, 299, 354, 891, 1144, 1464, 1520
MunI CAATTG 2 cut(s) 58, 965
Mva1269I GAATGC 3 cut(s) 1341, 1374, 1529
MvaI CCWGG 7 cut(s) 175, 299, 354, 891, 1144, 1464, 1520
MwoI GCNNNNNNNGC 6 cut(s) 38, 179, 194, 1016, 1184, 1380
NcoI CCATGG 1 cut(s) 1132
NdeII GATC 4 cut(s) 95, 214, 247, 718
NlaIII CATG 6 cut(s) 102, 787, 910, 1077, 1136, 1442
NlaIV GGNNCC 5 cut(s) 478, 793, 883, 1095, 1235
NmuCI GTSAC 2 cut(s) 469, 499
PciSI GCTCTTC 1 cut(s) 260
PctI GAATGC 3 cut(s) 1341, 1374, 1529
PfeI GAWTC 3 cut(s) 226, 534, 664
PflFI GACNNNGTC 1 cut(s) 1354
PfoI TCCNGGA 1 cut(s) 1518
PkrI GCNGC 6 cut(s) 9, 169, 172, 433, 583, 1012
PleI GAGTC 1 cut(s) 956
PpsI GAGTC 1 cut(s) 956
Ppu21I YACGTR 1 cut(s) 1540
PpuMI RGGWCCY 2 cut(s) 115, 1522
Psp5II RGGWCCY 2 cut(s) 115, 1522
Psp6I CCWGG 7 cut(s) 173, 297, 352, 889, 1142, 1462, 1518
PspEI GGTNACC 2 cut(s) 554, 563
PspFI CCCAGC 2 cut(s) 82, 668
PspGI CCWGG 7 cut(s) 173, 297, 352, 889, 1142, 1462, 1518
PspN4I GGNNCC 5 cut(s) 478, 793, 883, 1095, 1235
PspPI GGNCC 5 cut(s) 115, 477, 1070, 1234, 1522
PspPPI RGGWCCY 2 cut(s) 115, 1522
PstNI CAGNNNCTG 1 cut(s) 581
PsuI RGATCY 1 cut(s) 247
PsyI GACNNNGTC 1 cut(s) 1354
RsaI GTAC 3 cut(s) 677, 827, 1301
RsaNI GTAC 3 cut(s) 676, 826, 1300
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 8 cut(s) 324, 624, 704, 864, 1088, 1344, 1401, 1457
SatI GCNGC 6 cut(s) 8, 168, 171, 432, 582, 1011
Sau3AI GATC 4 cut(s) 95, 214, 247, 718
Sau96I GGNCC 5 cut(s) 115, 477, 1070, 1234, 1522
ScaI AGTACT 2 cut(s) 677, 827
SchI GAGTC 1 cut(s) 957
ScrFI CCNGG 7 cut(s) 175, 299, 354, 891, 1144, 1464, 1520
SduI GDGCHC 3 cut(s) 43, 1096, 1503
SfaNI GCATC 2 cut(s) 507, 1392
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 4 cut(s) 115, 477, 1070, 1522
SmlI CTYRAG 1 cut(s) 1400
SmoI CTYRAG 1 cut(s) 1400
SnaBI TACGTA 1 cut(s) 1540
SsiI CCGC 5 cut(s) 66, 170, 251, 332, 1013
SspMI CTAG 3 cut(s) 725, 1422, 1526
StyD4I CCNGG 7 cut(s) 173, 297, 352, 889, 1142, 1462, 1518
StyI CCWWGG 2 cut(s) 559, 1132
TaaI ACNGT 5 cut(s) 91, 496, 1157, 1367, 1448
TaiI ACGT 2 cut(s) 1322, 1542
TaqI TCGA 4 cut(s) 121, 213, 537, 1149
TatI WGTACW 2 cut(s) 675, 825
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 3 cut(s) 226, 534, 664
Tru1I TTAA 8 cut(s) 324, 624, 704, 864, 1088, 1344, 1401, 1457
Tru9I TTAA 8 cut(s) 324, 624, 704, 864, 1088, 1344, 1401, 1457
TscAI CASTG 2 cut(s) 474, 762
TseFI GTSAC 2 cut(s) 469, 499
TseI GCWGC 5 cut(s) 7, 167, 431, 581, 1010
Tsp45I GTSAC 2 cut(s) 469, 499
TspDTI ATGAA 5 cut(s) 17, 849, 873, 992, 1497
TspGWI ACGGA 3 cut(s) 121, 288, 750
TspRI CASTG 2 cut(s) 474, 762
Tth111I GACNNNGTC 1 cut(s) 1354
Vha464I CTTAAG 1 cut(s) 1400
VpaK11BI GGWCC 4 cut(s) 115, 477, 1070, 1522
XagI CCTNNNNNAGG 1 cut(s) 1241
XapI RAATTY 4 cut(s) 305, 438, 1484, 1492
XbaI TCTAGA 1 cut(s) 724
XspI CTAG 3 cut(s) 725, 1422, 1526
ZrmI AGTACT 2 cut(s) 677, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.